SitesBLAST
Comparing WP_034211124.1 NCBI__GCF_000747155.1:WP_034211124.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
30% identity, 36% coverage: 6:292/789 of query aligns to 1:278/282 of 4kugA
- active site: S117 (= S123), H138 (= H146), E150 (= E158), N188 (= N197)
- binding nicotinamide-adenine-dinucleotide: G10 (= G15), T11 (≠ V16), M12 (= M17), R30 (≠ F35), D31 (= D36), A87 (= A93), A88 (≠ I94), E90 (= E96), N115 (= N121), S117 (= S123), H138 (= H146)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
30% identity, 36% coverage: 6:292/789 of query aligns to 1:278/280 of 4kuhA
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
31% identity, 37% coverage: 3:295/789 of query aligns to 24:314/314 of P00348
- GGGLMG 34:39 (≠ GAGVMG 13:18) binding NAD(+)
- D57 (vs. gap) binding NAD(+)
- E122 (= E96) binding NAD(+)
- K127 (= K101) binding NAD(+)
- S149 (= S123) binding NAD(+)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
30% identity, 36% coverage: 6:292/789 of query aligns to 5:284/286 of P9WNP7
- S122 (= S123) mutation to A: Loss of fatty acyl dehydrogenase activity.
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
30% identity, 37% coverage: 3:295/789 of query aligns to 24:314/314 of Q16836
- GGGLMG 34:39 (≠ GAGVMG 13:18) binding NAD(+)
- A40 (= A19) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (vs. gap) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (≠ N54) binding CoA
- K80 (≠ A61) binding CoA
- L86 (≠ A67) to P: in dbSNP:rs4956145
- E122 (= E96) binding NAD(+)
- K127 (= K101) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S123) binding CoA; binding NAD(+)
- Q152 (≠ S126) to H: in dbSNP:rs1051519
- N173 (= N149) binding NAD(+)
- Y226 (≠ S203) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (≠ T236) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K286) binding NAD(+)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
30% identity, 37% coverage: 3:295/789 of query aligns to 1:291/291 of 1f0yA
- active site: S126 (= S123), H147 (= H146), E159 (= E158), N197 (= N197)
- binding acetoacetyl-coenzyme a: S50 (≠ N54), K57 (≠ A61), S126 (= S123), H147 (= H146), F149 (= F148), N150 (= N149), P151 (= P150), P153 (≠ R152), V154 (≠ Y153), N197 (= N197), P232 (= P232), M233 (≠ S234), L238 (≠ T239)
- binding nicotinamide-adenine-dinucleotide: G13 (= G15), L14 (≠ V16), M15 (= M17), D34 (vs. gap), Q35 (vs. gap), A96 (= A93), I97 (= I94), E99 (= E96), K104 (= K101), N124 (= N121), S126 (= S123), H147 (= H146), N150 (= N149), V242 (= V243), T246 (= T247), K282 (= K286)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
30% identity, 37% coverage: 3:295/789 of query aligns to 1:291/293 of 1f17A
- active site: S126 (= S123), H147 (= H146), E159 (= E158), N197 (= N197)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G15), L14 (≠ V16), M15 (= M17), D34 (vs. gap), Q35 (vs. gap), A96 (= A93), I97 (= I94), E99 (= E96), K104 (= K101), N124 (= N121), S126 (= S123), H147 (= H146)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
30% identity, 37% coverage: 3:295/789 of query aligns to 1:291/293 of 1f12A
- active site: S126 (= S123), H147 (= H146), E159 (= E158), N197 (= N197)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ A61), S126 (= S123), H147 (= H146), F149 (= F148), N150 (= N149), M155 (= M154), N197 (= N197), L200 (≠ G200), P232 (= P232), M233 (≠ S234), L238 (≠ T239)
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
30% identity, 37% coverage: 6:293/789 of query aligns to 2:280/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
30% identity, 37% coverage: 6:293/789 of query aligns to 2:280/283 of 4pzdA
- active site: S118 (= S123), H139 (= H146), E151 (= E158), N189 (= N197)
- binding nicotinamide-adenine-dinucleotide: G11 (= G15), T12 (≠ V16), M13 (= M17), D32 (= D36), A88 (= A93), A89 (≠ I94), T90 (≠ A95), E91 (= E96), I99 (≠ L104), N116 (= N121), S118 (= S123), N142 (= N149)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
27% identity, 50% coverage: 2:399/789 of query aligns to 287:659/692 of 6iunB
- active site: S407 (= S123), H428 (= H146), E440 (= E158), N478 (= N197)
- binding nicotinamide-adenine-dinucleotide: G300 (= G15), T301 (≠ V16), M302 (= M17), E321 (≠ D36), T322 (≠ L37), Y365 (vs. gap), A377 (= A93), V378 (≠ I94), E380 (= E96), V384 (≠ W100), V388 (≠ L104), N405 (= N121), S407 (= S123)
Sites not aligning to the query:
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 30% coverage: 87:322/789 of query aligns to 393:629/729 of P21177
- H450 (= H146) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
Sites not aligning to the query:
- 116 G→F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- 322 G→A: 10-fold increase in KM for NADH.
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
29% identity, 36% coverage: 7:292/789 of query aligns to 1:277/281 of 6aa8E
- active site: S116 (= S123), H137 (= H146), E149 (= E158), N187 (= N197)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ L12), G9 (= G15), T10 (≠ V16), M11 (= M17), R29 (≠ F35), D30 (= D36), I31 (≠ L37), A86 (= A93), A87 (≠ I94), E89 (= E96), K94 (= K101), N114 (= N121), S116 (= S123)
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
32% identity, 30% coverage: 87:322/789 of query aligns to 393:629/719 of 6tnmA
Sites not aligning to the query:
9jheA 3-hydroxybutyryl-coa dehydrogenase with NAD (see paper)
29% identity, 36% coverage: 8:293/789 of query aligns to 2:277/289 of 9jheA