SitesBLAST
Comparing WP_034268231.1 NCBI__GCF_000504245.1:WP_034268231.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P77455 Bifunctional protein PaaZ; EC 3.3.2.12; EC 1.2.1.91 from Escherichia coli (strain K12) (see paper)
51% identity, 99% coverage: 7:678/679 of query aligns to 4:676/681 of P77455
- E256 (= E259) mutation to Q: Catalyzes the formation of 3-oxo-5,6-dehydrosuberyl-CoA semialdehyde instead of 3-oxo-5,6-dehydrosuberyl-CoA.
6jqoA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ccoa (see paper)
51% identity, 99% coverage: 7:678/679 of query aligns to 3:675/678 of 6jqoA
- active site: N157 (= N161), E255 (= E259), C294 (= C298), L483 (≠ M491)
- binding crotonyl coenzyme a: V97 (≠ F101), F107 (= F111), S111 (= S115), F158 (= F162), W161 (= W165), R638 (vs. gap)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N154 (= N158), F156 (= F160), N157 (= N161), T183 (≠ S187), T230 (= T234), G231 (= G235), S232 (= S236), T235 (= T239), A256 (= A260), D257 (= D261), C294 (= C298)
6jqnA Structure of paaz, a bifunctional enzyme in complex with NADP+ and ocoa (see paper)
51% identity, 99% coverage: 7:678/679 of query aligns to 3:675/678 of 6jqnA
- active site: N157 (= N161), E255 (= E259), C294 (= C298), L483 (≠ M491)
- binding octanoyl-coenzyme a: F562 (= F569), H565 (= H572), F576 (= F584), G583 (= G591), V595 (= V603), A604 (= A612), N605 (= N613), Y606 (= Y614), F613 (= F621), I614 (≠ L622)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R19 (≠ T23), I153 (= I157), N154 (= N158), A155 (= A159), F156 (= F160), K180 (= K184), A182 (= A186), T183 (≠ S187), T230 (= T234), G231 (= G235), S232 (= S236), T235 (= T239), L239 (= L243), E255 (= E259), A256 (= A260), D257 (= D261), C294 (= C298), F396 (= F404), H471 (= H479)
6jqmA Structure of paaz with NADPH (see paper)
51% identity, 99% coverage: 7:678/679 of query aligns to 3:675/678 of 6jqmA
- active site: N157 (= N161), E255 (= E259), C294 (= C298), L483 (≠ M491)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R19 (≠ T23), I153 (= I157), N154 (= N158), A155 (= A159), F156 (= F160), N157 (= N161), K180 (= K184), A182 (= A186), T183 (≠ S187), G231 (= G235), S232 (= S236), T235 (= T239), A256 (= A260), D257 (= D261), C294 (= C298), E394 (= E402), F396 (= F404)
2vroA Crystal structure of aldehyde dehydrogenase from burkholderia xenovorans lb400 (see paper)
47% identity, 70% coverage: 7:479/679 of query aligns to 6:484/521 of 2vroA
- active site: N160 (= N161), K183 (= K184), E258 (= E259), C297 (= C298), E401 (= E402)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I156 (= I157), K183 (= K184), S217 (≠ A218), S235 (= S236), T238 (= T239), L242 (= L243), F403 (= F404)
Sites not aligning to the query:
2y53A Crystal structure of e257q mutant of the box pathway encoded aldh from burkholderia xenovorans lb400 (see paper)
46% identity, 70% coverage: 7:479/679 of query aligns to 6:483/529 of 2y53A
- active site: N160 (= N161), K183 (= K184), Q258 (≠ E259), C297 (= C298), E401 (= E402)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I156 (= I157), N157 (= N158), F159 (= F160), N160 (= N161), K183 (= K184), A185 (= A186), T186 (≠ S187), S217 (≠ A218), F232 (= F233), G234 (= G235), S235 (= S236), A236 (= A237), T238 (= T239), A259 (= A260), D260 (= D261), C297 (= C298), F403 (= F404)
Sites not aligning to the query:
4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
28% identity, 65% coverage: 8:445/679 of query aligns to 19:442/505 of 4neaA
- active site: N166 (= N161), K189 (= K184), E264 (≠ N264), C298 (= C298), E399 (= E402)
- binding nicotinamide-adenine-dinucleotide: P164 (≠ A159), K189 (= K184), E192 (≠ S187), G222 (vs. gap), G226 (= G219), G242 (= G235), G243 (≠ S236), T246 (= T239), H249 (≠ Q242), I250 (= I249), C298 (= C298), E399 (= E402), F401 (= F404)
Sites not aligning to the query:
3b4wA Crystal structure of mycobacterium tuberculosis aldehyde dehydrogenase complexed with NAD+
29% identity, 65% coverage: 65:506/679 of query aligns to 67:475/483 of 3b4wA