SitesBLAST
Comparing WP_034269044.1 NCBI__GCF_000504245.1:WP_034269044.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3b4wA Crystal structure of mycobacterium tuberculosis aldehyde dehydrogenase complexed with NAD+
52% identity, 100% coverage: 3:481/481 of query aligns to 4:480/483 of 3b4wA
- active site: N154 (= N154), K177 (= K177), E251 (= E251), C285 (= C285), E384 (= E385), E460 (= E461)
- binding nicotinamide-adenine-dinucleotide: I150 (= I150), V151 (≠ T151), W153 (= W153), N154 (= N154), K177 (= K177), I210 (≠ R210), G213 (= G213), T228 (= T228), G229 (= G229), S230 (= S230), V233 (≠ A233), E236 (≠ R236), E251 (= E251), L252 (= L252), C285 (= C285), E384 (= E385), F386 (= F387)
5gtlA NADPH complex structure of aldehyde dehydrogenase from bacillus cereus
40% identity, 100% coverage: 1:480/481 of query aligns to 13:490/491 of 5gtlA
- active site: N165 (= N154), K188 (= K177), E263 (= E251), C297 (= C285), E394 (= E385), E471 (= E461)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I161 (= I150), P163 (= P152), K188 (= K177), A190 (≠ P179), E191 (≠ P180), Q192 (≠ E181), G221 (= G209), G225 (= G213), G241 (= G229), S242 (= S230), T245 (≠ A233), L264 (= L252), C297 (= C285), E394 (= E385), F396 (= F387)
5gtkA NAD+ complex structure of aldehyde dehydrogenase from bacillus cereus
40% identity, 100% coverage: 1:480/481 of query aligns to 13:490/491 of 5gtkA
- active site: N165 (= N154), K188 (= K177), E263 (= E251), C297 (= C285), E394 (= E385), E471 (= E461)
- binding nicotinamide-adenine-dinucleotide: I161 (= I150), I162 (≠ T151), P163 (= P152), W164 (= W153), K188 (= K177), E191 (≠ P180), G221 (= G209), G225 (= G213), A226 (≠ E214), F239 (= F227), G241 (= G229), S242 (= S230), T245 (≠ A233), Y248 (≠ R236), L264 (= L252), C297 (= C285), Q344 (= Q332), R347 (= R335), E394 (= E385), F396 (= F387)
Q56YU0 Aldehyde dehydrogenase family 2 member C4; ALDH1a; Protein REDUCED EPIDERMAL FLUORESCENCE 1; EC 1.2.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 99% coverage: 3:480/481 of query aligns to 18:495/501 of Q56YU0
- G152 (= G134) mutation to E: In ref1-7; reduced activity on sinapaldehyde.
- G416 (≠ A402) mutation to R: In ref1-6; reduced activity on sinapaldehyde.
4pxlA Structure of zm aldh2-3 (rf2c) in complex with NAD (see paper)
39% identity, 99% coverage: 4:480/481 of query aligns to 5:480/486 of 4pxlA
- active site: N154 (= N154), K177 (= K177), E253 (= E251), C287 (= C285), E384 (= E385), D461 (≠ E461)
- binding nicotinamide-adenine-dinucleotide: I150 (= I150), V151 (≠ T151), P152 (= P152), W153 (= W153), K177 (= K177), E180 (≠ P180), G210 (= G209), G214 (= G213), A215 (≠ E214), F228 (= F227), G230 (= G229), S231 (= S230), V234 (≠ A233), E253 (= E251), G255 (= G253), C287 (= C285), Q334 (= Q332), K337 (≠ R335), E384 (= E385), F386 (= F387)
8rwkA Cryoem structure of the central ald4 filament determined by filamentid (see paper)
38% identity, 99% coverage: 3:476/481 of query aligns to 16:489/495 of 8rwkA
4pz2B Structure of zm aldh2-6 (rf2f) in complex with NAD (see paper)
40% identity, 99% coverage: 4:480/481 of query aligns to 10:488/494 of 4pz2B
- active site: N159 (= N154), K182 (= K177), E258 (= E251), C292 (= C285), E392 (= E385), D469 (≠ E461)
- binding nicotinamide-adenine-dinucleotide: I155 (= I150), I156 (≠ T151), P157 (= P152), W158 (= W153), N159 (= N154), M164 (≠ V159), K182 (= K177), A184 (≠ P179), E185 (≠ P180), G215 (= G209), G219 (= G213), F233 (= F227), T234 (= T228), G235 (= G229), S236 (= S230), V239 (≠ A233), E258 (= E251), L259 (= L252), C292 (= C285), E392 (= E385), F394 (= F387)
3ty7B Crystal structure of aldehyde dehydrogenase family protein from staphylococcus aureus
39% identity, 92% coverage: 1:441/481 of query aligns to 2:436/454 of 3ty7B
P17202 Aminoaldehyde dehydrogenase BADH; 4-trimethylammoniobutyraldehyde dehydrogenase BADH; Aminobutyraldehyde dehydrogenase BADH; Betaine aldehyde dehydrogenase; SoBADH; EC 1.2.1.-; EC 1.2.1.47; EC 1.2.1.19; EC 1.2.1.8 from Spinacia oleracea (Spinach) (see 3 papers)
37% identity, 98% coverage: 5:476/481 of query aligns to 8:482/497 of P17202
- I28 (= I25) binding K(+)
- D96 (≠ E93) binding K(+)
- SPW 156:158 (≠ TPW 151:153) binding NAD(+)
- Y160 (≠ V155) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-(trimethylamino)butanal.
- W167 (≠ A162) mutation to A: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- KPSE 182:185 (≠ KPPP 177:180) binding NAD(+)
- L186 (≠ E181) binding K(+)
- SSAT 236:239 (≠ STAA 230:233) binding NAD(+)
- V251 (≠ L245) binding in other chain
- L258 (= L252) binding NAD(+)
- W285 (≠ L279) mutation to A: Decreases binding affinity for betaine aldehyde.
- E390 (= E385) binding NAD(+)
- A441 (≠ T436) mutation to I: Decreases binding affinity for betaine aldehyde; increases binding affinity for 4-aminobutanal.
- C450 (≠ D445) mutation to S: Loss of partial inactivation by betaine aldehyde in the absence of NAD(+).
- W456 (≠ F450) binding NAD(+); mutation to A: Decreases binding affinity for betaine aldehyde.
- K460 (= K454) binding K(+)
6x9lA Crystal structure of aldehyde dehydrogenasE C (aldc) mutant (c291a) from pseudomonas syringae in complexed with NAD+ and octanal (see paper)
43% identity, 98% coverage: 5:476/481 of query aligns to 12:477/485 of 6x9lA