SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_034281422.1 NCBI__GCF_000691165.1:WP_034281422.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
43% identity, 20% coverage: 473:594/619 of query aligns to 17:138/161 of 1glcF

query
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1glcF
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1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
43% identity, 20% coverage: 473:594/619 of query aligns to 6:127/150 of 1o2fA

query
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1o2fA
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P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
43% identity, 20% coverage: 473:594/619 of query aligns to 25:146/169 of P69783

query
sites
P69783
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Sites not aligning to the query:

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
35% identity, 22% coverage: 473:607/619 of query aligns to 503:648/648 of P09323

query
sites
P09323
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Sites not aligning to the query:

Query Sequence

>WP_034281422.1 NCBI__GCF_000691165.1:WP_034281422.1
MDYTQVAKDVLQHIGGKENIAHLEHCSTRLRFTLIDQKKADVPALEKTPGVIAVRMSGQC
QVVIGNDVIEVYQKVTSLIGSSSSSQDVPSNQPKEKRKAGTVLLDFIVGVFQPLVPAIAG
GGILKSFLLLFSLLGWMDAKGQTYQILNMVGDAPLYFLPLLVAVTTANKLKVNPLVALSA
VGALLLPNMTAMLTEGAQLLSFDVKNIAYAYQVFPAILSVLLYAQMEKFFTRFSPKPIRI
FFVPMMSLVITVPVTLLLLGPIGFTAGQGFSSIILAMFNTVGWVAVAILAAVLPFMVASG
MHKAMVPYAVTTMGTLGKEALYLPASLAHNIAESGACFAVALRTKDKVLRSTAISAGISA
FFGITEPALYGVTLQNKRVLGSVMIGSFVGGIFIGLVGIQAFVLVGPGLASMSMFISDEL
PRNLMFAIIGAVISFIVAFIAAFVLGKDRTVEEKTKDQAAFAEQIGAGETFKSPVIGQMI
SLSEVKDDIFSSKVMGDGIAIVPSKGALYAPVDGEVTLLFETNHALGMKTDQGVEVLFHI
GIDTVQLEGQYFYPKVQAGDRVQAGDLLIEFDLEKIMEAGYDPVTLAVITNTDQYDIKVQ
PLQEVNRQDTLMVVTQLGG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory