SitesBLAST
Comparing WP_034526014.1 NCBI__GCF_000740055.1:WP_034526014.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
45% identity, 89% coverage: 1:230/257 of query aligns to 1:231/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (= C6), A7 (≠ I7), K8 (= K8), D38 (= D37), T61 (≠ S59), M62 (= M60), A93 (= A91), D94 (= D92), T95 (= T93), T98 (= T96), L102 (= L100), G118 (= G116), Q120 (= Q118), G124 (≠ A122), T126 (= T124), A127 (≠ G125), V129 (= V127), T223 (= T222)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
43% identity, 89% coverage: 1:229/257 of query aligns to 1:229/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (= C6), I7 (= I7), K8 (= K8), D38 (= D37), M61 (= M60), A92 (= A91), D93 (= D92), T94 (= T93), T97 (= T96), A115 (≠ F115), G116 (= G116), A119 (≠ S119), G122 (≠ A122), T124 (= T124), A125 (≠ G125), V127 (= V127)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
43% identity, 99% coverage: 1:255/257 of query aligns to 1:255/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ I7), K8 (= K8), Y37 (= Y36), D38 (= D37), Y40 (≠ N39), T60 (≠ S59), M61 (= M60), A92 (= A91), D93 (= D92), T94 (= T93), T97 (= T96), M101 (≠ L100), C116 (≠ F115), G117 (= G116), Q119 (= Q118), A120 (≠ S119), G123 (≠ A122), T125 (= T124), A126 (≠ G125), V128 (= V127)
- binding nicotinamide-adenine-dinucleotide: M61 (= M60), G62 (= G61), P63 (= P62), S85 (= S84), D86 (≠ S85), G91 (= G90)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
43% identity, 99% coverage: 1:255/257 of query aligns to 1:255/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ I7), K8 (= K8), Y37 (= Y36), D38 (= D37), Y40 (≠ N39), M61 (= M60), A92 (= A91), D93 (= D92), T94 (= T93), T97 (= T96), C116 (≠ F115), G117 (= G116), Q119 (= Q118), A120 (≠ S119), G123 (≠ A122), T125 (= T124), A126 (≠ G125), V128 (= V127)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
47% identity, 86% coverage: 3:224/257 of query aligns to 4:232/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (= C6), I8 (= I7), K9 (= K8), D39 (= D37), M62 (= M60), A93 (= A91), D94 (= D92), T95 (= T93), T98 (= T96), L102 (= L100), T121 (≠ F115), G122 (= G116), A125 (≠ S119), G128 (≠ A122), T130 (= T124), G131 (= G125), V133 (= V127)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
40% identity, 89% coverage: 2:231/257 of query aligns to 3:235/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (= C6), K9 (= K8), D39 (= D37), M62 (= M60), D94 (= D92), V95 (≠ T93), L102 (= L100), G118 (= G116), Q120 (= Q118), T121 (≠ S119), T126 (= T124), A127 (≠ G125), V129 (= V127)
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
31% identity, 100% coverage: 1:256/257 of query aligns to 1:262/264 of P53570
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
31% identity, 100% coverage: 1:256/257 of query aligns to 1:261/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ C6), V7 (≠ I7), K8 (= K8), D38 (= D37), V63 (vs. gap), V99 (≠ T96), A117 (≠ F115), G118 (= G116), Q120 (= Q118), S121 (= S119), Y126 (≠ T124), A127 (≠ G125), T129 (≠ V127)
- binding flavin-adenine dinucleotide: Q120 (= Q118), Q182 (≠ R178)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
33% identity, 82% coverage: 1:211/257 of query aligns to 1:214/246 of 1efpB
- binding adenosine monophosphate: P6 (≠ C6), V7 (≠ I7), D39 (= D37), I63 (≠ M60), V101 (≠ T96), A119 (≠ F115), G120 (= G116), Q122 (= Q118), A123 (≠ S119), N126 (≠ A122), M128 (≠ T124), N129 (≠ G125), T131 (≠ V127)
- binding flavin-adenine dinucleotide: Y13 (≠ G13), L182 (≠ A179)
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
32% identity, 82% coverage: 1:211/257 of query aligns to 1:214/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ C6), V7 (≠ I7), K8 (= K8), C39 (≠ D37), C63 (≠ M60), V101 (≠ T93), L119 (≠ F115), G120 (= G116), Q122 (= Q118), A123 (≠ S119), D126 (≠ A122), C128 (≠ T124), N129 (≠ G125), Q130 (= Q126), T131 (≠ V127)
- binding flavin-adenine dinucleotide: P37 (≠ T35), L182 (≠ A179)
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
32% identity, 77% coverage: 15:211/257 of query aligns to 20:217/255 of Q9DCW4
- K200 (≠ T194) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ E197) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
32% identity, 82% coverage: 1:211/257 of query aligns to 4:217/255 of P38117
- A9 (≠ C6) binding AMP
- NPFC 39:42 (≠ NTYD 34:37) binding AMP
- C66 (≠ M60) binding AMP
- 123:134 (vs. 116:127, 42% identical) binding AMP
- D128 (= D121) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ E147) to M: in dbSNP:rs1130426
- R164 (= R158) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ L159) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 177:199, 35% identical) Recognition loop
- L195 (≠ P189) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (≠ T194) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (≠ TSF 194:196) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ TSFE 194:197) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (≠ E197) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
33% identity, 77% coverage: 15:211/257 of query aligns to 20:217/255 of Q2TBV3
- K200 (≠ T194) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (≠ E197) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
34% identity, 74% coverage: 1:189/257 of query aligns to 1:203/251 of 5ow0B
- binding adenosine monophosphate: L6 (≠ C6), A7 (≠ I7), K8 (= K8), D39 (= D37), Y62 (≠ M60), I95 (≠ T96), A114 (≠ F115), G115 (= G116), Q117 (= Q118), S118 (= S119), R123 (≠ T124), G124 (= G125), V125 (≠ Q126), V126 (= V127), P127 (≠ G128)
- binding flavin-adenine dinucleotide: L37 (≠ T35), Y38 (= Y36)
Query Sequence
>WP_034526014.1 NCBI__GCF_000740055.1:WP_034526014.1
MKIVVCIKQVPVGDVKIDPKTNNLARSNVEGDINTYDRNAIEAALQLKEQAGGEVILLSM
GPDSYMSSLRDGLAMGTDSAVLMSSRAFGGADTLATAYTLSEGIKKIGGVDLILFGRQSV
DADTGQVGPIVAEDLNIPQITFAEGVELHDDNVLVGTRLLDNSVQEVKVTLPAVLTVRAE
MNKPRYETPLNIQTSFEKPVTVWSEKDLDLDESRIGQAGSPTIVRKVYSPDKAAKQIEML
PQNAAAAVTKLLTELNN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory