SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_034529054.1 NCBI__GCF_000740055.1:WP_034529054.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4daeA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 6-chloroguanosine (see paper)
62% identity, 98% coverage: 1:231/235 of query aligns to 1:230/232 of 4daeA

query
sites
4daeA

M

M

S

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H

L

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E

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A

A

K

E

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K

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G

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Y

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I

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E

E

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T

F

Y

L

L

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E

N

N

P

V

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E

R

C

Y

Y

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N

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R

N

G

A

M

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Y

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G

Y

F

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T

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Y

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S

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Q

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M

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G

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P

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Y

Y

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E
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E

L

L

I

I

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E

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Y

Y

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D

V

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T

N

L

L

F

I

R
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R

V

V

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G

S
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S

S
x
C

G
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G

G

A

M

I

S

R

P

K

D

D

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H

K

V

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R

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D

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I

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T

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D

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M

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R

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A

F

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G

-

G

G

G

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I

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Y

D

F

F

A

A

P

P

I

C

A

A

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D

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F

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E

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A

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G

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S

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F

Y

Y

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N

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M

S

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Q

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I

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E

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K

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Y

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G

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E

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E
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E

A

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A

A

L

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Y

L

T

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A

A

K

K

Y

H

H

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R

R

K

A

A

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A

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I

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T

I

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S
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S

N
x
D

H

H

L
x
V

V

L

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T

G

G

E

E

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T

A

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active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S202 (= S203), D203 (≠ N204), V205 (≠ L206), R216 (= R217)
binding 6-chloro-9-(beta-D-ribofuranosyl)-9H-purin-2-amine: M64 (= M64), S90 (= S90), C91 (≠ S91), G92 (= G92), F159 (= F160), E178 (= E179), M179 (= M180), E180 (= E181), S202 (= S203)

4darA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with tubercidin (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4darA

query
sites
4darA

S

S

E

V

H
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H

L

I

E

G

A

A

K

E

K

K

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G

D

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I

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A

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T

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V

L

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P

G
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G

D

D

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P

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L

R
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R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
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R

N

G

A

M

F

Y

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G

Y

F

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Y

Y

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G

K

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K

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Q

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M

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G

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Y

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N

N

E
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E

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L

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S

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Y

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D

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V

K

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T

N

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R

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G

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S

S
x
C

G
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G

G

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M

I

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K

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D

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K

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R

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S

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M

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R

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V

T

A

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F

G

-

G

G

G

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Y

D

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F

A

A

P

P

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C

A

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E

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E

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M

E
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E

A

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A

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L

Y

Y

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L

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A

K

K

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R

K

A

A

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A

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T

I

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S
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S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

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R

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T

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D

M

M

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K

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H

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S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding '2-(4-amino-pyrrolo[2,3-d]pyrimidin-7-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: S89 (= S90), C90 (≠ S91), G91 (= G92), F158 (= F160), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)

4dabA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with hypoxanthine (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4dabA

query
sites
4dabA

S

S

E

V

H
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H

L

I

E

G

A

A

K

E

K

K

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G

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I

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A

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T

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V

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P

G
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G

D

D

P

P

L

L

R
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R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
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R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

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V

Q

Q

G

G

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T

G

G

M

M

G

G

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P

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S

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I

S

S

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I

Y

Y

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N

N

E
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E

L

L

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I

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Q

E

S

Y

Y

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D

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V

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Q

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R

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G

G

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M

I

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D

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K

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R

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M

G

T

A

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S

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I

M

I

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H

R

N

V

T

A

F

F

G

-

G

G

G

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I

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Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

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E

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A

A

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Y

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D

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F
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F

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Y

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N

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I

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E

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x
V

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E

M
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M

E

E

A

T

A

A

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L

Y

Y

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T

L

L

A

A

K

K

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H

H

G

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R

R

K

A

A

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L

A

S

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I

L

L

T

T

I

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S
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S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

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A

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R

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T

F

F

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S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding hypoxanthine: G91 (= G92), F158 (= F160), V176 (≠ T178), M178 (= M180)

4da8A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 8-bromoguanosine (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4da8A

query
sites
4da8A

S

S

E

V

H
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H

L

I

E

G

A

A

K

E

K

K

G

G

D

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T

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G

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R

A

A

K

K

Y

F

I

I

A

A

E

E

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T

F

Y

L

L

E

E

N

N

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V

V

E

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C

Y

Y

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N

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R

N

G

A

M

F

Y

G

G

Y

F

T

T

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G

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T

Y

Y

K

K

G

G

K

K

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K

I

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Q

G

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G

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M

G

G

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P

S

S

I

I

S

S

I

I

Y

Y

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N

N

E
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E

L

L

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I

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E

S

Y

Y

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D

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V

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L

L

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R

V

V

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G

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S

S

C

G
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G

G

A

M

I

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K

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D

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R

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D

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G

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A

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T

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D

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S

S

Q

I

M

I

N

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R

N

V

T

A

F

F

G

-

G

G

G

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V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

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A

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Y

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D

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G

N

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F

S

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A

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D

R

Q

F
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F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E
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E

M
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M

E
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E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
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S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
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R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding 8-bromoguanosine: S89 (= S90), G91 (= G92), F158 (= F160), V176 (≠ T178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)

4da7A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with aciclovir (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4da7A

query
sites
4da7A

S

S

E

V

H
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H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

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A

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T

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V

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P

G
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G

D

D

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P

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R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

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V

R
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R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

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G

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M

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G

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P

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S

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S

S

I

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Y

Y

T

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N

N

E
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E

L

L

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Q

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Y

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D

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V

K

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L

L

F

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R
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R

V

V

G

G

S
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S

S
x
C

G
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G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

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V

I

I

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L

A

A

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M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

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N

A

A

Y

Y

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D

A

A

Q

K

D

D

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K

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G

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S

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S

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F
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F

Y

Y

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N

D

D

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D

M

S

D

Q

R

I

E

E

K

K

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V

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Y

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V

A

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A

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T
x
V

E

E

M
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M

E
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E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
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S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
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R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding 9-hyroxyethoxymethylguanine: C90 (≠ S91), G91 (= G92), F158 (= F160), V176 (≠ T178), M178 (= M180), E179 (= E181)
binding phosphate ion: G19 (= G20), R86 (= R87), S89 (= S90)

4da6A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with ganciclovir (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4da6A

query
sites
4da6A

S

S

E

V

H
|
H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

A

A

D

D

T

T

V

V

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
|
R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

S

S

V

V

Q

Q

G

G

T

T

G

G

M

M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

D

V

V

K

Q

T

N

L

L

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G
|
G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

L

A

A

Q

M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

K

T

N

A

A

Y

Y

H

D

A

A

Q

K

D

D

L

K

D

G

I

V

P

P

V

V

R

T

V

V

G

G

N

S

V

V

L

F

S

T

E

A

D

D

R

Q

F
|
F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E

E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding 9-(1,3-dihydroxy-propoxymethane)guanine: S89 (= S90), C90 (≠ S91), G91 (= G92), F158 (= F160), V176 (≠ T178), M178 (= M180), E179 (= E181), S201 (= S203)

4d9hA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with adenosine (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4d9hA

query
sites
4d9hA

S

S

E

V

H
|
H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

A

A

D

D

T

T

V

V

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
|
R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

S

S

V

V

Q

Q

G

G

T

T

G

G

M
|
M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

D

V

V

K

Q

T

N

L

L

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G
|
G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

L

A

A

Q

M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

K

T

N

A

A

Y

Y

H

D

A

A

Q

K

D

D

L

K

D

G

I

V

P

P

V

V

R

T

V

V

G

G

N

S

V

V

L

F

S

T

E

A

D

D

R

Q

F
|
F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E

E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding adenosine: M63 (= M64), S89 (= S90), C90 (≠ S91), G91 (= G92), F158 (= F160), V176 (≠ T178), M178 (= M180), E179 (= E181), S201 (= S203)

4d8vA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis at ph 4.2 (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/231 of 4d8vA

query
sites
4d8vA

S

S

E

V

H
|
H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

A

A

D

D

T

T

V

V

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
|
R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

S

S

V

V

Q

Q

G

G

T

T

G

G

M

M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

D

V

V

K

Q

T

N

L

L

F

I

R
|
R

V

V

G

G

S
|
S

S

C

G
|
G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

L

A

A

Q

M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

K

T

N

A

A

Y

Y

H

D

A

A

Q

K

D

D

L

K

D

G

I

V

P

P

V

V

R

T

V

V

G

G

N

S

V

V

L

F

S

T

E

A

D

D

R

Q

F
|
F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E
|
E

M
|
M

E

E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding adenine: G91 (= G92), F158 (= F160), V176 (≠ T178), E177 (= E179), M178 (= M180)

4da0A Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 2'-deoxyguanosine (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/230 of 4da0A

query
sites
4da0A

S

S

E

V

H
|
H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

A

A

D

D

T

T

V

V

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
|
R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

S

S

V

V

Q

Q

G

G

T

T

G

G

M

M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

D

V

V

K

Q

T

N

L

L

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G
|
G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

L

A

A

Q

M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

K

T

N

A

A

Y

Y

H

D

A

A

Q

K

D

D

L

K

D

G

I

V

P

P

V

V

R

T

V

V

G

G

N

S

V

V

L

F

S

T

E

A

D

D

R

Q

F
|
F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E
|
E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding 2'-deoxy-guanosine: S89 (= S90), C90 (≠ S91), G91 (= G92), F158 (= F160), V176 (≠ T178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)

4danA Crystal structure of the hexameric purine nucleoside phosphorylase from bacillus subtilis in complex with 2-fluoroadenosine (see paper)
62% identity, 98% coverage: 2:231/235 of query aligns to 1:229/229 of 4danA

query
sites
4danA

S

S

E

V

H
|
H

L

I

E

G

A

A

K

E

K

K

G

G

D

Q

I

I

A

A

D

D

T

T

V

V

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

F

I

I

A

A

E

E

T

T

F

Y

L

L

E

E

N

N

P

V

V

E

R

C

Y

Y

N

N

T

E

V

V

R
|
R

N

G

A

M

F

Y

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

K

I

I

S

S

V

V

Q

Q

G

G

T

T

G

G

M

M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

D

V

V

K

Q

T

N

L

L

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G
|
G

G

A

M

I

S

R

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

L

A

A

Q

M

G

T

A

S

T

S

T

T

D

D

S

S

S

Q

I

M

I

N

H

R

N

V

T

A

F

F

G

-

G

G

G

S

I

V

Y

D

F

F

A

A

P

P

I

C

A

A

D

D

F

F

G

E

L

L

D

K

T

N

A

A

Y

Y

H

D

A

A

Q

K

D

D

L

K

D

G

I

V

P

P

V

V

R

T

V

V

G

G

N

S

V

V

L

F

S

T

E

A

D

D

R

Q

F
|
F

Y

Y

N

N

D

D

E

D

M

S

D

Q

R

I

E

E

K

K

L

L

V

A

Q

K

Y

Y

G

G

V

V

A

L

A

G

T
x
V

E
|
E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

H

H

G

V

R

R

K

A

A

L

L

A

S

V

I

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
V

V

L

T

T

G

G

E

E

T

T

A

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

H

D

D

M

M

I

I

K

D

V

V

A

A

L

L

E

H

T

S

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), S89 (= S90), S201 (= S203), D202 (≠ N204), V204 (≠ L206), R215 (= R217)
binding 2-(6-amino-2-fluoro-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: H3 (= H4), R42 (= R43), S89 (= S90), C90 (≠ S91), G91 (= G92), F158 (= F160), V176 (≠ T178), E177 (= E179), M178 (= M180), E179 (= E181), S201 (= S203)

3uawA Crystal structure of adenosine phosphorylase from bacillus cereus complexed with adenosine (see paper)
61% identity, 99% coverage: 2:234/235 of query aligns to 1:233/233 of 3uawA

query
sites
3uawA

S

S

E

V

H
|
H

L

I

E

E

A

A

K

K

K

Q

G

G

D

E

I

I

A

A

D

E

T

S

V

I

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

V

V

T

R

C

Y

Y

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

R

I

V

S

S

V

V

Q

Q

G

G

T

T

G

G

M
|
M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

G

V

V

K

K

T

N

L

L

F

I

R
|
R

V

V

G
|
G

S
x
T

S
x
C

G
|
G

G

A

M

I

S

Q

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

I

A

A

Q

M

G

T

A

A

T

C

T

T

D

D

S

S

S

N

I

M

I

N

H

R

N

L

T

T

F

F

G

-

G

P

G

G

I

F

Y

D

F

F

A

A

P

P

I

A

A

A

D

N

F

F

G

D

L

L

D

K

T

K

A

A

Y

Y

H

D

A

A

A

G

Q

T

D

E

L

K

D

G

I

L

P

H

V

V

R

R

V

V

G

G

N

N

V

V

L

L

S

T

E

A

D

D

R

V

F
|
F

Y

Y

N

R

D

E

E

S

M

M

D

D

R

M

-

V

E

K

K

K

L

L

V

G

Q

D

Y

Y

G

G

V

V

A

L

A

A

T

V

E
|
E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

Y

H

G

V

V

R

N

A

A

L

L

A

S

V

V

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

F

T

T

G

G

E

E

T

T

A

S

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

E

M

M

I

I

K

E

V

I

A

A

L

L

E

D

T

A

A

A

V

I

N

Q

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), T89 (≠ S90), S202 (= S203), D203 (≠ N204), I205 (≠ L206), R216 (= R217)
binding adenosine: M63 (= M64), T89 (≠ S90), C90 (≠ S91), G91 (= G92), F158 (= F160), E178 (= E179), M179 (= M180), E180 (= E181), D203 (≠ N204)
binding sulfate ion: G19 (= G20), R86 (= R87), G88 (= G89), T89 (≠ S90)

3uaxA Crystal structure of adenosine phosphorylase from bacillus cereus complexed with inosine (see paper)
59% identity, 99% coverage: 2:233/235 of query aligns to 1:226/226 of 3uaxA

query
sites
3uaxA

S

S

E

V

H
|
H

L

I

E

E

A

A

K

K

K

Q

G

G

D

E

I

I

A

A

D

E

T

S

V

I

L

L

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

V

V

T

R

C

Y

Y

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

K

R

R

I

V

S

S

V

V

Q

Q

G

G

T

T

G

G

M
|
M

G

G

I

V

P

P

S

S

I

I

S

S

I

I

Y

Y

T

V

N

N

E
|
E

L

L

I

I

T

Q

E

S

Y

Y

G

G

V

V

K

K

T

N

L

L

F

I

R
|
R

V

V

G
|
G

S
x
T

S
x
C

G
|
G

G

A

M

I

S

Q

P

K

D

D

V

V

H

K

V

V

R

R

D

D

V

V

I

I

L

I

A

A

Q

M

G

T

A

A

T

C

T

T

D

D

S

S

S

N

I

M

I

N

H

R

N

L

T

T

F

F

G

-

G

P

G

G

I

F

Y

D

F

F

A

A

P

P

I

A

A

A

D

N

F

F

G

D

L

L

D

K

T

K

A

A

Y

Y

H

D

A

A

A

G

Q

T

D

E

L

K

D

G

I

L

P

H

V

V

R

R

V

V

G

G

N

N

V

V

L

L

S

T

E

A

D

D

R

V

F
|
F

Y

Y

N

R

D

E

E

S

M

M

D

D

R

M

-

V

E

K

K

K

L

L

V

G

Q

D

Y

Y

G

G

V

V

A

L

A

A

T

V

E
|
E

M
|
M

E
|
E

A

T

A

A

L

L

Y

Y

L

T

L

L

A

A

K

K

Y

Y

H

G

V

V

R

N

A

A

L

L

A

S

V

V

L

L

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

F

T

S

G

-

E

-

T

-

A

-

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

E

M

M

I

I

K

E

V

I

A

A

L

L

E

D

T

A

A

A

V

I

active site: H3 (= H4), G19 (= G20), R23 (= R24), R42 (= R43), E74 (= E75), R86 (= R87), T89 (≠ S90), S202 (= S203), D203 (≠ N204), I205 (≠ L206), R210 (= R217)
binding inosine: M63 (= M64), T89 (≠ S90), C90 (≠ S91), G91 (= G92), F158 (= F160), E178 (= E179), M179 (= M180), E180 (= E181)
binding sulfate ion: G19 (= G20), R86 (= R87), G88 (= G89), T89 (≠ S90)

9fpeA Purine nucleoside phosphorylase DeoD-type (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 9fpeA

query
sites
9fpeA

S

T

E

P

H

H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G

G

D

D

P

P

L

L

R

R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R

R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M

M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E

E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R

R

V

V

G

G

S

S

S
x
C

G

G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D

F

F

A

A

P

A

I

I

A

A

D

D

F

F

G

D

L

M

L

V

D

R

T

N

A

A

Y

V

H

D

A

A

Q

K

D

A

L

L

D

G

I

I

P

D

V

A

R

R

V

V

G

G

N

N

V

L

L

F

S

S

E

A

D

D

R

L

F
|
F

Y

Y

N

S

D

P

E

D

M

G

D

E

R

M

-

F

E

D

K

V

L

M

V

E

Q

K

Y

Y

G

G

V

I

A

L

A

G

T
x
V

E
|
E

M
|
M

E

E

A

A

A

G

L

I

Y

Y

L

G

L

V

A

A

K

E

Y

F

H

G

V

A

R

K

A

A

L

L

A

T

V

I

L

C

T

T

I

V

S

S

N
x
D

H

H

L
x
I

V

R

T

T

G

H

E

E

E

Q

T

T

A

A

E

A

E

E

R

R

E

Q

K

T

S

T

F

F

D

N

D

D

M

M

I

I

K

K

V

I

A

A

L

L

E

E

T

S

A

V

V

L

N

L

G

G

binding N,2,3-etheno-2-aminopurine: C91 (≠ S91), F159 (= F160), V178 (≠ T178), E179 (= E179), M180 (= M180), D204 (≠ N204), I206 (≠ L206)

5iu6A Crystal structure of e.Coli purine nucleoside phosphorylase with 7- deazahypoxanthine (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 5iu6A

query
sites
5iu6A

S

T

E

P

H
|
H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M

M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E
|
E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G
|
G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D

F

F

A

A

P

A

I

I

A

A

D

D

F

F

G

D

L

M

L

V

D

R

T

N

A

A

Y

V

H

D

A

A

Q

K

D

A

L

L

D

G

I

I

P

D

V

A

R

R

V

V

G

G

N

N

V

L

L

F

S

S

E

A

D

D

R

L

F
|
F

Y

Y

N

S

D

P

E

D

M

G

D

E

R

M

-

F

E

D

K

V

L

M

V

E

Q

K

Y

Y

G

G

V

I

A

L

A

G

T
x
V

E
|
E

M

M

E

E

A

A

A

G

L

I

Y

Y

L

G

L

V

A

A

K

E

Y

F

H

G

V

A

R

K

A

A

L

L

A

T

V

I

L

C

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

R

T

T

G

H

E

E

E

Q

T

T

A

A

E

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

D

M

M

I

I

K

K

V

I

A

A

L

L

E

E

T

S

A

V

V

L

N

L

G

G

active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (≠ N204), I206 (≠ L206), R217 (= R217)
binding 7H-pyrrolo[2,3-d]pyrimidin-4-ol: C91 (≠ S91), G92 (= G92), F159 (= F160), V178 (≠ T178), E179 (= E179)

5i3cA Crystal structure of e.Coli purine nucleoside phosphorylase with acycloguanosine (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 5i3cA

query
sites
5i3cA

S

T

E

P

H
|
H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M

M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E
|
E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R
|
R

V

V

G
|
G

S
|
S

S
x
C

G
|
G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D

F

F

A

A

P

A

I

I

A

A

D

D

F

F

G

D

L

M

L

V

D

R

T

N

A

A

Y

V

H

D

A

A

Q

K

D

A

L

L

D

G

I

I

P

D

V

A

R

R

V

V

G

G

N

N

V

L

L

F

S

S

E

A

D

D

R

L

F
|
F

Y

Y

N

S

D

P

E

D

M

G

D

E

R

M

-

F

E

D

K

V

L

M

V

E

Q

K

Y

Y

G

G

V

I

A

L

A

G

T
x
V

E
|
E

M

M

E

E

A

A

A

G

L

I

Y

Y

L

G

L

V

A

A

K

E

Y

F

H

G

V

A

R

K

A

A

L

L

A

T

V

I

L

C

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

R

T

T

G

H

E

E

E

Q

T

T

A

A

E

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

D

M

M

I

I

K

K

V

I

A

A

L

L

E

E

T

S

A

V

V

L

N

L

G

G

active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (≠ N204), I206 (≠ L206), R217 (= R217)
binding 9-hyroxyethoxymethylguanine: S90 (= S90), C91 (≠ S91), G92 (= G92), F159 (= F160), V178 (≠ T178), E179 (= E179), I206 (≠ L206)
binding sulfate ion: G20 (= G20), R87 (= R87), G89 (= G89), S90 (= S90)

3ut6A Crystal structure of e. Coli pnp complexed with po4 and formycin a (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 3ut6A

query
sites
3ut6A

S

T

E

P

H
|
H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M
|
M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E
|
E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R
|
R

V

V

G
|
G

S
|
S

S
x
C

G
|
G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D

F

F

A

A

P

A

I

I

A

A

D

D

F

F

G

D

L

M

L

V

D

R

T

N

A

A

Y

V

H

D

A

A

Q

K

D

A

L

L

D

G

I

I

P

D

V

A

R

R

V

V

G

G

N

N

V

L

L

F

S

S

E

A

D

D

R

L

F
|
F

Y

Y

N

S

D

P

E

D

M

G

D

E

R

M

-

F

E

D

K

V

L

M

V

E

Q

K

Y

Y

G

G

V

I

A

L

A

G

T
x
V

E
|
E

M
|
M

E
|
E

A

A

A

G

L

I

Y

Y

L

G

L

V

A

A

K

E

Y

F

H

G

V

A

R

K

A

A

L

L

A

T

V

I

L

C

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

R

T

T

G

H

E

E

E

Q

T

T

A

A

E

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

D

M

M

I

I

K

K

V

I

A

A

L

L

E

E

T

S

A

V

V

L

N

L

G

G

active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (≠ N204), I206 (≠ L206), R217 (= R217)
binding (1S)-1-(7-amino-1H-pyrazolo[4,3-d]pyrimidin-3-yl)-1,4-anhydro-D-ribitol: M64 (= M64), S90 (= S90), C91 (≠ S91), G92 (= G92), F159 (= F160), V178 (≠ T178), E179 (= E179), M180 (= M180), E181 (= E181), D204 (≠ N204)
binding phosphate ion: G20 (= G20), R24 (= R24), R87 (= R87), G89 (= G89), S90 (= S90)

1pw7A Crystal structure of e. Coli purine nucleoside phosphorylase complexed with 9-beta-d-arabinofuranosyladenine and sulfate/phosphate (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 1pw7A

query
sites
1pw7A

S

T

E

P

H
|
H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M

M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E
|
E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R
|
R

V

V

G

G

S
|
S

S
x
C

G

G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D

F

F

A

A

P

A

I

I

A

A

D

D

F

F

G

D

L

M

L

V

D

R

T

N

A

A

Y

V

H

D

A

A

Q

K

D

A

L

L

D

G

I

I

P

D

V

A

R

R

V

V

G

G

N

N

V

L

L

F

S

S

E

A

D

D

R

L

F
|
F

Y

Y

N

S

D

P

E

D

M

G

D

E

R

M

-

F

E

D

K

V

L

M

V

E

Q

K

Y

Y

G

G

V

I

A

L

A

G

T

V

E
|
E

M

M

E
|
E

A

A

A

G

L

I

Y

Y

L

G

L

V

A

A

K

E

Y

F

H

G

V

A

R

K

A

A

L

L

A

T

V

I

L

C

T

T

I

V

S
|
S

N
x
D

H

H

L
x
I

V

R

T

T

G

H

E

E

E

Q

T

T

A

A

E

A

E

E

R
|
R

E

Q

K

T

S

T

F

F

D

N

D

D

M

M

I

I

K

K

V

I

A

A

L

L

E

E

T

S

A

V

V

L

N

L

G

G

active site: H4 (= H4), G20 (= G20), R24 (= R24), R43 (= R43), E75 (= E75), R87 (= R87), S90 (= S90), S203 (= S203), D204 (≠ N204), I206 (≠ L206), R217 (= R217)
binding phosphate ion: G20 (= G20), R87 (= R87), S90 (= S90)
binding 2-(6-amino-purin-9-yl)-5-hydroxymethyl-tetrahydro-furan-3,4-diol: S90 (= S90), C91 (≠ S91), F159 (= F160), E179 (= E179), E181 (= E181), S203 (= S203)

1pr0A Escherichia coli purine nucleoside phosphorylase complexed with inosine and phosphate/sulfate (see paper)
51% identity, 100% coverage: 2:235/235 of query aligns to 2:235/237 of 1pr0A

query
sites
1pr0A

S

T

E

P

H
|
H

L

I

E

N

A

A

K

E

K

M

G

G

D

D

I

F

A

A

D

D

T

V

V

V

L

L

L

M

P

P

G
|
G

D

D

P

P

L

L

R
|
R

A

A

K

K

Y

Y

I

I

A

A

E

E

T

T

F

F

L

L

E

E

N

D

P

A

V

R

R

E

Y

V

N

N

T

N

V

V

R
|
R

N

G

A

M

F

L

G

G

Y

F

T

T

G

G

T

T

Y

Y

K

K

G

G

K

R

R

K

I

I

S

S

V

V

Q

M

G

G

T

H

G

G

M

M

G

G

I

I

P

P

S

S

I

C

S

S

I

I

Y

Y

T

T

N

K

E
|
E

L

L

I

I

T

T

E

D

Y

F

G

G

V

V

K

K

T

K

L

I

F

I

R
|
R

V

V

G
|
G

S
|
S

S
x
C

G

G

G

A

M

V

S

L

P

P

D

H

V

V

H

K

V

L

R

R

D

D

V

V

I

V

L

I

A

G

Q

M

G

G

A

A

T

C

T

T

D

D

S

S

S

K

I

V

I

N

H

R

N

I

T

R

F

F

G

K

G

D

G

H

I

-

Y

D