SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035129577.1 NCBI__GCF_000769915.1:WP_035129577.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

H1ZZB0 4-hydroxy-2-methyl-3-oxo-4-farnesyl-3,4-dihydroquinoline-1-oxide ketoreductase; Aurachin B dehydrogenase; EC 1.1.1.394 from Stigmatella aurantiaca (see paper)
28% identity, 59% coverage: 1:172/293 of query aligns to 1:181/334 of H1ZZB0

query
sites
H1ZZB0

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S111 (≠ F94) mutation to A: Does not produce aurachin B.
Y139 (≠ A130) mutation to F: Does not produce aurachin B.

8rdhA Crystal structure of udp-galactose 4-epimerase from pyrococcus horikoshii with bound NAD and gdp-l-fucose
29% identity, 96% coverage: 1:281/293 of query aligns to 1:300/319 of 8rdhA

query
sites
8rdhA

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binding guanosine-5'-diphosphate-beta-l-fucopyranose: R83 (≠ S78), S120 (≠ G110), Y145 (≠ P133), N174 (≠ I158), G183 (= G166), V184 (≠ F167), D187 (≠ I170), F188 (≠ V171), K191 (≠ M174), L202 (≠ I184), Q207 (≠ N189), K209 (≠ W191), V248 (≠ F226), W277 (= W255), D280 (≠ H258)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ V12), D31 (≠ V31), D32 (≠ R32), S34 (≠ Q34), A35 (= A35), G36 (≠ S36), M57 (≠ I52), L76 (≠ T71), A77 (= A72), A78 (≠ F73), P80 (≠ H75), T118 (= T108), Y145 (≠ P133), K149 (vs. gap), L172 (= L156), A173 (≠ P157), N174 (≠ I158), I175 (≠ V159), H182 (= H165)

P95780 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
26% identity, 99% coverage: 3:292/293 of query aligns to 7:322/348 of P95780

query
sites
P95780

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FI 15:16 (≠ FV 11:12) binding NAD(+)
DKLT 37:40 (≠ EVLG 26:29) binding NAD(+)
DI 62:63 (= DI 51:52) binding NAD(+)
YAAES 82:86 (≠ TAF-N 71:74) binding NAD(+)
N88 (≠ D76) binding substrate
T101 (vs. gap) binding NAD(+)
T125 (= T95) binding substrate
N190 (≠ I158) binding substrate
N191 (≠ V159) binding NAD(+)
KFIPRQ 200:205 (≠ GFIPIV 166:171) binding substrate
KLY 216:218 (≠ GYI 182:184) binding substrate
R225 (≠ W191) binding substrate
N260 (≠ F226) binding substrate

D5JWB3 Sanguinarine reductase; EC 1.3.1.107 from Eschscholzia californica (California poppy) (see paper)
41% identity, 24% coverage: 1:69/293 of query aligns to 8:77/273 of D5JWB3

query
sites
D5JWB3

M

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Sites not aligning to the query:

102:114 mutation Missing: Loss of catalytic activity.
153 S→A: Loss of catalytic activity.
157 C→A: Strongly decreased catalytic activity.
158 D→N: Slightly decreased catalytic activity.
161 H→A: Strongly decreased catalytic activity.
162 F→P: Slightly decreased catalytic activity.
166 M→L: No effect on catalytic activity.

1kerB The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-d-glucose bound (see paper)
25% identity, 98% coverage: 2:288/293 of query aligns to 5:314/347 of 1kerB

query
sites
1kerB

R

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P

R

E

M

Q

G

V

E

V

T

F

Y

H

L

P

I

I

G

A

A

E

D

E

G

G

E

V

K

P

N

F
x
N

K

K

E

E

I

V

A

L

R

E

V

L

I

I

S

L

E

E

N

K

T

M

A

G

F

Q

P

P

L

-

K

-

S

-

L

-

T

-

E

-

A

K

E

D

A

A

E

Y

A

D

H

H

F

-

T

-

W

-

F

V

T

T

H

D

F
x
R

A

A

G

G

M
x
H

D

D

-

L

A

R

Y

Y

A

A

-

I

S

D

S

A

E

S

K

K

T

L

Q

R

N

D

I

E

L

L

G

G

W

W

K

T

P

P

E

Q

Q

F

I

T

G

D

L

F

L

S

E

E

D

G

L

L

active site: T124 (= T95), D125 (= D96), E126 (≠ A97), Y160 (≠ A130), K164 (≠ R134)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S85 (vs. gap), N87 (vs. gap), T124 (= T95), D125 (= D96), E126 (≠ A97), Y160 (≠ A130), N189 (≠ I158), K199 (≠ G166), F200 (= F167), R203 (≠ I170), Q204 (≠ V171), K215 (≠ G182), L216 (≠ Y183), Y217 (≠ I184), R224 (≠ W191), N259 (≠ F226), R283 (≠ F259), H286 (≠ M262)
binding nicotinamide-adenine-dinucleotide: G13 (= G10), F14 (= F11), I15 (≠ V12), D36 (≠ E26), K37 (≠ V27), L38 (= L28), T39 (≠ G29), G42 (≠ R32), D61 (= D51), I62 (= I52), Y81 (vs. gap), A82 (vs. gap), A83 (vs. gap), S85 (vs. gap), T100 (= T71), S123 (≠ F94), T124 (= T95), Y160 (≠ A130), K164 (≠ R134), C187 (≠ L156), N190 (≠ V159)

1ketA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with thymidine diphosphate bound (see paper)
25% identity, 98% coverage: 2:288/293 of query aligns to 4:313/346 of 1ketA

query
sites
1ketA

R

N

V

I

F

I

V

V

T

T

G

G

A

G

S

A

G
|
G

F
|
F

V
x
I

G

G

S

S

A

N

V

F

V

V

N

H

-

Y

-

V

-

Y

-

N

-

H

-

P

D

D

L

V

I

H

A

V

A

T

G

V

I

L

E
x
D

V
x
K

L
|
L

G
x
T

L

Y

V
x
A

R
x
G

S

N

Q

K

A

A

S

N

A

L

E

E

K

A

L

I

K

L

D

G

S

D

G

R

A

V

Q

E

I

L

L

V

M

V

G

G

D
|
D

I
|
I

N

A

D

D

P

A

E

E

I

L

I

V

R

D

K

K

G

L

A

A

T

A

S

K

C

A

D

D

A

A

V

I

-

V

-

H

-
x
Y

-

A

-
x
A

-

E

-
x
S

-

H

-

N

-

D

-

N

-

S

-

L

-

N

-

D

-

P

-

S

-

P

-

F

I

I

H

H

T
|
T

A

N

F

F

N

I

H

G

D

T

F

Y

S

T

R

L

Y

L

K

E

E

A

S

A

C

R

E

K

D

Y

D

D

R

I

K

R

V

F

I

H

E

H

I

V

F
x
S

T
|
T

D
|
D

A
x
E

L

V

I

Y

G

G

T

D

Q

L

K

P

P

L

L

R

V

E

I

D

T

L

S

P

G

G

V

H

G

G

I

E

L

G

R

P

S

G

E

E

K

K

A

F

I

T

T

A

E

E

D

T

D

N

-

Y

T

N

P

P

T

S

S

S

S

P

A
x
Y

S

S

V

S

P

T

R
x
K

A

A

S

S

E

D

E

L

-

I

A

V

A

K

L

A

E

W

A

V

R

R

S

S

K

F

G

G

V

V

N

K

A

A

Y

T

I

I

V

S

R

N

L

C

P

S

I
x
N

V

N

H

Y

G

G

K

P

D

Y

D

Q

H

H

-

I

-

E

G

K

F
|
F

I

I

P

P

I
x
R

V
x
Q

I

I

G

T

M

N

D

I

K

L

E

A

N

G

G

I

Q

K

S

P

G
x
K

Y

L

I
x
Y

G

G

D

E

G

G

L

K

N

N

R

V

W
x
R

P

D

G

W

V

I

H

H

R

T

E

N

D

D

A

H

A

S

S

T

L

G

Y

V

R

W

L

A

I

I

V

L

E

T

K

K

Q

G

P

R

E

M

Q

G

V

E

V

T

F

Y

H

L

P

I

I

G

A

A

E

D

E

G

G

E

V

K

P

N

F
x
N

K

K

E

E

I

V

A

L

R

E

V

L

I

I

S

L

E

E

N

K

T

M

A

G

F

Q

P

P

L

-

K

-

S

-

L

-

T

-

E

-

A

K

E

D

A

A

E

Y

A

D

H

H

F

-

T

-

W

-

F

V

T

T

H

D

F
x
R

A

A

G

G

M
x
H

D

D

-

L

A

R

Y

Y

A

A

-

I

S

D

S

A

E

S

K

K

T

L

Q

R

N

D

I

E

L

L

G

G

W

W

K

T

P

P

E

Q

Q

F

I

T

G

D

L

F

L

S

E

E

D

G

L

L

active site: T123 (= T95), D124 (= D96), E125 (≠ A97), Y159 (≠ A130), K163 (≠ R134)
binding nicotinamide-adenine-dinucleotide: G12 (= G10), F13 (= F11), I14 (≠ V12), D35 (≠ E26), K36 (≠ V27), L37 (= L28), T38 (≠ G29), A40 (≠ V31), G41 (≠ R32), D60 (= D51), I61 (= I52), Y80 (vs. gap), A82 (vs. gap), S84 (vs. gap), T99 (= T71), S122 (≠ F94), T123 (= T95), Y159 (≠ A130), K163 (≠ R134)
binding thymidine-5'-diphosphate: E125 (≠ A97), N188 (≠ I158), F199 (= F167), R202 (≠ I170), Q203 (≠ V171), K214 (≠ G182), Y216 (≠ I184), R223 (≠ W191), N258 (≠ F226), R282 (≠ F259), H285 (≠ M262)

1kepA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from streptococcus suis with dtdp-xylose bound (see paper)
25% identity, 98% coverage: 2:288/293 of query aligns to 4:313/346 of 1kepA

query
sites
1kepA

R

N

V

I

F

I

V

V

T

T

G

G

A

G

S

A

G
|
G

F
|
F

V
x
I

G

G

S

S

A

N

V

F

V

V

N

H

-

Y

-

V

-

Y

-

N

-

H

-

P

D

D

L

V

I

H

A

V

A

T

G

V

I

L

E
x
D

V
x
K

L
|
L

G
x
T

L

Y

V

A

R
x
G

S

N

Q

K

A

A

S

N

A

L

E

E

K

A

L

I

K

L

D

G

S

D

G

R

A

V

Q

E

I

L

L

V

M

V

G

G

D
|
D

I
|
I

N

A

D

D

P

A

E

E

I

L

I

V

R

D

K

K

G

L

A

A

T

A

S

K

C

A

D

D

A

A

V

I

-

V

-

H

-
x
Y

-
x
A

-

E

-
x
S

-

H

-

N

-

D

-

N

-

S

-

L

-

N

-

D

-

P

-

S

-

P

-

F

I

I

H

H

T
|
T

A

N

F

F

N

I

H

G

D

T

F

Y

S

T

R

L

Y

L

K

E

E

A

S

A

C

R

E

K

D

Y

D

D

R

I

K

R

V

F

I

H

E

H

I

V

F
x
S

T
|
T

D
|
D

A
x
E

L

V

I

Y

G

G

T

D

Q

L

K

P

P

L

L

R

V

E

I

D

T

L

S

P

G

G

V

H

G

G

I

E

L

G

R

P

S

G

E

E

K

K

A

F

I

T

T

A

E

E

D

T

D

N

-

Y

T

N

P

P

T

S

S

S

S

P

A
x
Y

S

S

V

S

P

T

R
x
K

A

A

S

S

E

D

E

L

-

I

A

V

A

K

L

A

E

W

A

V

R

R

S

S

K

F

G

G

V

V

N

K

A

A

Y

T

I

I

V

S

R

N

L

C

P

S

I
x
N

V
x
N

H

Y

G

G

K

P

D

Y

D

Q

H

H

-

I

-

E

G
x
K

F

F

I

I

P

P

I

R

V

Q

I

I

G

T

M

N

D

I

K

L

E

A

N

G

G

I

Q

K

S

P

G

K

Y

L

I

Y

G

G

D

E

G

G

L

K

N

N

R

V

W
x
R

P

D

G

W

V

I

H

H

R

T

E

N

D

D

A

H

A

S

S

T

L

G

Y

V

R

W

L

A

I

I

V

L

E

T

K

K

Q

G

P

R

E

M

Q

G

V

E

V

T

F

Y

H

L

P

I

I

G

A

A

E

D

E

G

G

E

V

K

P

N

F

N

K

K

E

E

I

V

A

L

R

E

V

L

I

I

S

L

E

E

N

K

T

M

A

G

F

Q

P

P

L

-

K

-

S

-

L

-

T

-

E

-

A

K

E

D

A

A

E

Y

A

D

H

H

F

-

T

-

W

-

F

V

T

T

H

D

F
x
R

A

A

G

G

M

H

D

D

-

L

A

R

Y

Y

A

A

-

I

S

D

S

A

E

S

K

K

T

L

Q

R

N

D

I

E

L

L

G

G

W

W

K

T

P

P

E

Q

Q

F

I

T

G

D

L

F

L

S

E

E

D

G

L

L

active site: T123 (= T95), D124 (= D96), E125 (≠ A97), Y159 (≠ A130), K163 (≠ R134)
binding nicotinamide-adenine-dinucleotide: G12 (= G10), F13 (= F11), I14 (≠ V12), D35 (≠ E26), K36 (≠ V27), L37 (= L28), T38 (≠ G29), G41 (≠ R32), D60 (= D51), I61 (= I52), Y80 (vs. gap), A81 (vs. gap), A82 (vs. gap), S84 (vs. gap), T99 (= T71), S122 (≠ F94), Y159 (≠ A130), K163 (≠ R134), N189 (≠ V159)
binding thymidine-5'-diphospho-beta-d-xylose: S84 (vs. gap), T123 (= T95), E125 (≠ A97), Y159 (≠ A130), N188 (≠ I158), K198 (≠ G166), R223 (≠ W191), R282 (≠ F259)

6nbrA Crystal structure of piper methysticum kavalactone reductase 1 in complex with NADP (see paper)
38% identity, 24% coverage: 2:72/293 of query aligns to 2:79/316 of 6nbrA

query
sites
6nbrA

R

R

V

I

F

C

V

V

T

T

G
|
G

A

A

S

G

G
|
G

F
|
F

V
|
V

G

A

S

S

A

W

V

V

N

K

D

L

L

F

I

L

A

S

A

K

G

G

I

Y

E

L

V

V

L

H

G

G

L

T

V

V

R
|
R

-

D

-

L

-

G

S

E

Q

E

A

K

S

T

A

A

E

H

K

L

L

R

K

K

D

L

S

E

G

G

A

A

-

Y

-

H

-

N

-

L

Q

Q

I

L

L

F

M

K

G

A

D
|
D

I
x
L

N

L

D

D

P

Y

E

E

I

S

I

L

R

L

K

G

G

A

A

I

T

T

S

G

C

C

D

D

A

G

V

V

I

L

H

H

T
x
V

A

A

binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G7), G10 (= G10), F11 (= F11), V12 (= V12), R32 (= R32), D58 (= D51), L59 (≠ I52), V78 (≠ T71)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 80, 82, 120, 122, 159, 163, 186, 187, 189, 201

Q9NRG7 Epimerase family protein SDR39U1; Short-chain dehydrogenase/reductase family 39U member 1; EC 1.1.1.- from Homo sapiens (Human) (see 2 papers)
23% identity, 80% coverage: 1:233/293 of query aligns to 1:232/293 of Q9NRG7

query
sites
Q9NRG7

M

M

R

R

V

V

F

L

V

V

T

G

G

G

A

G

S

T

G

G

F

F

V

I

G

G

S

T

A

A

V

L

V

T

N

Q

D

L

L

L

I

N

A

A

A

R

G

G

I

H

E

E

V

V

L

T

G

L

L

V

V
x
S

R
|
R

S

K

Q

P

A

G

S

P

A

-

E

-

K

-

L

-

K

-

D

-

S

-

G

-

A

-

Q

-

I

-

L

-

M

-

G

G

D

R

I

I

N

T

D

W

P

D

E

E

I

L

I

A

R

A

K

S

G

G

A

L

T

P

S

S

C

C

D

D

A

A

V

A

I

V

H

N

T
x
L

A
|
A

-

G

-

E

-

N

F

I

N

L

H

N

D

P

F

L

S

R

R

R

Y

W

K

N

E

E

S

T

C

F

E

Q

D

K

D
x
E

-

V

-
x
I

-

G

-

S

-
x
R

R

L

K

E

V

T

I

T

E

Q

I

L

F

L

T

A

D

K

A

A

L

I

I

T

G

K

T

A

Q

P

K

Q

P

P

-

P

-

K

-

A

L

W

V

V

I

L

T

V

S

T

G

G

V

V

G

A

I

Y

L

Y

R

Q

-

P

S

S

E

L

K

T

A

A

I

E

T

Y

E

D

D

E

D

D

T

S

P

P

T

G

S

G

S

D

A

F

S

D

V

F

-

F

-

S

P

N

R

L

A

V

A

T

S

K

E

W

E

E

A

A

L

A

E

R

A

L

R

P

S

G

K

D

G

S

V

T

N

R

A

Q

Y

V

I

V

V

V

R

R

L

S

P

G

I

V

V
|
V

H

L

G

G

K

R

D

G

-

G

-

A

-

M

D

G

H

H

G

M

F

L

I

L

P

P

I

F

V

R

I

L

G

G

M

L

D

-

K

-

E

-

N

-

G

-

Q

-

S

G

G
|
G

Y

P

I

I

G

G

D

S

G

G

L

H

N

Q

R

F

W

F

P

P

G

W

V

I

H

H

R

I

E

G

D

D

A

L

A

A

S

G

L

I

Y

L

R

T

L

H

I

A

V

L

E

E

K

A

Q

N

P

H

E

V

Q

H

V

G

V

V

F

L

H

N

P

G

I

V

A

A

E

P

E

S

G

S

V

A

P

T

F

N

K

A

E

E

I

F

A

A

R

Q

V

T

I
x
L

SR 31:32 (≠ VR 31:32) binding NADP(+)
LA 58:59 (≠ TA 71:72) binding NADP(+)
E77 (≠ D87) binding NADP(+)
I79 (vs. gap) to L: in dbSNP:rs11625819
R82 (vs. gap) binding NADP(+)
V160 (= V159) binding NADP(+)
G181 (= G182) to V: in dbSNP:rs11538256
L232 (≠ I233) to F: in dbSNP:rs3211056

Sites not aligning to the query:

270 Q → R: in dbSNP:rs1043831

3bxxA Binding of two substrate analogue molecules to dihydroflavonol 4- reductase alters the functional geometry of the catalytic site (see paper)
27% identity, 50% coverage: 3:149/293 of query aligns to 3:164/324 of 3bxxA

query
sites
3bxxA

V

V

F

C

V

V

T

T

G

G

A

A

S
|
S

G
|
G

F
|
F

V
x
I

G

G

S

S

A

W

V

L

V

V

N

M

D

R

L

L

I

L

A

E

A

R

G

G

I

Y

E

T

V

V

L

R

G

A

L

T

V

V

R
|
R

S

D

Q

P

A

T

S

N

A

V

E

K

K
|
K

L

V

K

K

-

H

-

L

-

D

-

L

-

P

-

K

-

A

D

E

S

T

G

H

A

L

Q

T

I

L

L

W

M

K

G

A

D
|
D

I
x
L

N

A

D

D

P

E

E

G

I

S

I

F

R

D

K

E

G

A

A

I

T

K

S

G

C

C

D

T

A

G

V

V

I

F

H

H

T
x
V

A

A

F
x
T

N
x
P

H

M

D

D

F

F

S

-

R

-

Y

-

K

-

E

E

S

S

C

K

E

D

D

P

D

E

R

N

K

E

V

V

I

I

E

K

I

P

F

T

T

I

D

E

A

G

L

M

I

L

G

G

T

I

Q

M

K

K

P

S

-

C

-

A

-

K

-

T

-

V

-

R

L

L

V

V

I

F

T

T

S

S

G
x
S

V

A

G
|
G

I

T

-

V

-

N

L

I

R

Q

S

E

E

H

K

Q

A

L

I

P

T

V

E

Y

D

D

D

E

T

S

P

C

T

W

S

S

S

D

A

M

S

E

V

F

P

C

R

R

A

A

A

K

S

K

E

M

E

T

A

A

A

W

L

M

E
x
Y

A
x
F

R

V

S

S

K

K

G

T

V

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: S9 (= S9), G10 (= G10), F11 (= F11), I12 (≠ V12), R32 (= R32), K39 (= K39), D59 (= D51), L60 (≠ I52), V79 (≠ T71), T81 (≠ F73), P82 (≠ N74)
binding 3,5,7,3',4'-pentahydroxyflavone: S123 (≠ G110), G125 (= G112), Y158 (≠ E143), F159 (≠ A144)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 185, 187, 188, 199, 200

2nnlD Binding of two substrate analogue molecules to dihydroflavonol-4- reductase alters the functional geometry of the catalytic site
27% identity, 50% coverage: 3:149/293 of query aligns to 3:164/324 of 2nnlD

query
sites
2nnlD

V

V

F

C

V

V

T

T

G

G

A

A

S
|
S

G
|
G

F
|
F

V
x
I

G

G

S

S

A

W

V

L

V

V

N

M

D

R

L

L

I

L

A

E

A

R

G

G

I

Y

E

T

V

V

L

R

G

A

L

T

V

V

R
|
R

S

D

Q

P

A

T

S

N

A

V

E

K

K
|
K

L

V

K

K

-

H

-

L

-

D

-

L

-

P

-

K

-

A

D

E

S

T

G

H

A

L

Q

T

I

L

L

W

M

K

G

A

D
|
D

I
x
L

N

A

D

D

P

E

E

G

I

S

I

F

R

D

K

E

G

A

A

I

T

K

S

G

C

C

D

T

A

G

V

V

I

F

H

H

T
x
V

A
|
A

F
x
T

N

P

H

M

D

D

F
|
F

S

-

R

-

Y

-

K

-

E

E

S

S

C

K

E

D

D

P

D

E

R

N

K

E

V

V

I

I

E

K

I

P

F

T

T

I

D

E

A

G

L

M

I

L

G

G

T

I

Q

M

K

K

P

S

-

C

-

A

-

K

-

T

-

V

-

R

L

L

V

V

I

F

T
|
T

S

S

G

S

V
x
A

G

G

I

T

-

V

-
x
N

L

I

R

Q

S

E

E

H

K

Q

A

L

I

P

T

V

E

Y

D

D

D

E

T

S

P

C

T

W

S

S

S

D

A

M

S

E

V

F

P

C

R

R

A

A

A

K

S

K

E

M

E

T

A

A

A

W

L

M

E
x
Y

A

F

R

V

S

S

K
|
K

G

T

V

L

binding (2s)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydro-4h-chromen-4-one: F85 (= F77), A124 (≠ V111), N128 (vs. gap), Y158 (≠ E143)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S9 (= S9), G10 (= G10), F11 (= F11), I12 (≠ V12), R32 (= R32), K39 (= K39), D59 (= D51), L60 (≠ I52), V79 (≠ T71), A80 (= A72), T81 (≠ F73), T121 (= T108), Y158 (≠ E143), K162 (= K147)

Sites not aligning to the query:

2c29D Structure of dihydroflavonol reductase from vitis vinifera at 1.8 a. (see paper)
27% identity, 50% coverage: 3:149/293 of query aligns to 3:164/324 of 2c29D

query
sites
2c29D

V

V

F

C

V

V

T

T

G

G

A

A

S
|
S

G
|
G

F
|
F

V
x
I

G

G

S

S

A

W

V

L

V

V

N

M

D

R

L

L

I

L

A

E

A

R

G

G

I

Y

E

T

V

V

L

R

G

A

L

T

V

V

R
|
R

S

D

Q

P

A

T

S

N

A

V

E

K

K
|
K

L

V

K

K

-

H

-

L

-

D

-

L

-

P

-

K

-

A

D

E

S

T

G

H

A

L

Q

T

I

L

L

W

M

K

G

A

D
|
D

I
x
L

N

A

D

D

P

E

E

G

I

S

I

F

R

D

K

E

G

A

A

I

T

K

S

G

C

C

D

T

A

G

V

V

I

F

H

H

T
x
V

A
|
A

F
x
T

N

P

H

M

D

D

F

F

S

-

R

-

Y

-

K

-

E

E

S

S

C

K

E

D

D

P

D

E

R

N

K

E

V

V

I

I

E

K

I

P

F

T

T

I

D

E

A

G

L

M

I

L

G

G

T

I

Q

M

K

K

P

S

-

C

-

A

-

K

-

T

-

V

-

R

L

L

V

V

I

F

T
|
T

S

S

G
x
S

V
x
A

G

G

I

T

-

V

-
x
N

L

I

R

Q

S

E

E

H

K

Q

A

L

I

P

T

V

E

Y

D

D

D

E

T

S

P

C

T

W

S

S

S

D

A

M

S

E

V

F

P

C

R

R

A

A

A

K

S

K

E

M

E

T

A

A

A

W

L

M

E
x
Y

A

F

R

V

S

S

K
|
K

G

T

V

L

binding (2r,3r)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-2,3-dihydro-4h-chromen-4-one: S123 (≠ G110), A124 (≠ V111), N128 (vs. gap), Y158 (≠ E143)
binding nadp nicotinamide-adenine-dinucleotide phosphate: S9 (= S9), G10 (= G10), F11 (= F11), I12 (≠ V12), R32 (= R32), K39 (= K39), D59 (= D51), L60 (≠ I52), V79 (≠ T71), A80 (= A72), T81 (≠ F73), T121 (= T108), Y158 (≠ E143), K162 (= K147)

Sites not aligning to the query:

4b4oA Crystal structure of human epimerase family protein sdr39u1 (isoform2) with NADPH
23% identity, 80% coverage: 1:233/293 of query aligns to 1:232/295 of 4b4oA

query
sites
4b4oA

M

M

R

R

V

V

F

L

V

V

T

G

G

G

A

G

S
x
T

G
|
G

F
|
F

V
x
I

G

G

S

T

A

A

V

L

V

T

N

Q

D

L

L

L

I

N

A

A

A

R

G

G

I

H

E

E

V

V

L

T

G

L

L

V

V
x
S

R
|
R

S

K

Q

P

A

G

S

P

A

-

E

-

K

-

L

-

K

-

D

-

S

-

G

-

A

-

Q

-

I

-

L

-

M

-

G

G

D

R

I

I

N

T

D

W

P

D

E

E

I

L

I

A

R

A

K

S

G

G

A

L

T

P

S

S

C

C

D

D

A

A

V

A

I

V

H

N

T
x
L

A
|
A

-
x
G

-
x
E

-

N

F

I

N

L

H

N

D

P

F

L

S

R

R

R

Y

W

K

N

E

E

S

T

C

F

E

Q

D

K

D

E

-

V

-

L

-

G

-
x
S

-
x
R

R

L

K

E

V

T

I

T

E

Q

I

L

F

L

T

A

D

K

A

A

L

I

I

T

G

K

T

A

Q

P

K

Q

P

P

-

P

-

K

-

A

L

W

V

V

I

L

T
x
V

S
x
T

G

G

V

V

G

A

I

Y

L

Y

R

Q

-

P

S

S

E

L

K

T

A

A

I

E

T

Y

E

D

D

E

D

D

T

S

P

P

T

G

S

G

S

D

A

F

S

D

V

F

-

F

-

S

P

N

R

L

A

V

A

T

S

K

E
x
W

E

E

A

A

L

A

E

R

A

L

R

P

S

G

K

D

G

S

V

T

N

R

A

Q

Y

V

I

V

V

V

R

R

L
x
S

P

G

I

V

V
|
V

H

L

G

G

K

R

D

G

-

G

-

A

-

M

D

G

H

H

G

M

F

L

I

L

P

P

I

F

V

R

I

L

G

G

M

L

D

-

K

-

E

-

N

-

G

-

Q

-

S

G

G

G

Y

P

I

I

G

G

D

S

G

G

L

H

N

Q

R

F

W

F

P

P

G

W

V

I

H

H

R

I

E

G

D

D

A

L

A

A

S

G

L

I

Y

L

R

T

L

H

I

A

V

L

E

E

K

A

Q

N

P

H

E

V

Q

H

V

G

V

V

F

L

H

N

P

G

I

V

A

A

E

P

E

S

G

S

V

A

P

T

F

N

K

A

E

E

I

F

A

A

R

Q

V

T

I

F

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: T9 (≠ S9), G10 (= G10), F11 (= F11), I12 (≠ V12), S31 (≠ V31), R32 (= R32), L58 (≠ T71), A59 (= A72), G60 (vs. gap), E61 (vs. gap), S81 (vs. gap), R82 (vs. gap), V106 (≠ T108), T107 (≠ S109), W139 (≠ E138), S157 (≠ L156), V160 (= V159)

1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
21% identity, 99% coverage: 1:291/293 of query aligns to 1:307/322 of 1r66A

query
sites
1r66A

M

M

R

R

V

L

F

L

V

V

T

T

G

G

A

G

S

A

G
|
G

F
|
F

V
x
I

G

G

S

S

A

H

V

F

V

V

N

R

D

Q

L

L

I

L

A

A

A

G

G

A

-

Y

-

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

-

V

I

L

E
x
D

V
x
S

L
|
L

G
x
T

L

Y

V

A

R
x
G

S

N

Q

R

A

A

S

N

A

L

E

A

K

P

L

V

K

D

-

A

D

D

S

P

G

R

A

L

Q

R

I

F

L

V

M

H

G

G

D
|
D

I
|
I

N

R

D

D

P

A

E

G

I

L

R

A

K

R

G

E

A

L

T

R

S

G

C

V

D

D

A

A

V

I

I

V

H

H

T
x
F

A
|
A

F
x
A

N

E

H
x
S

D
x
H

F

V

S

D

R

R

Y

S

K

I

E

A

S

G

C

A

E

-

D

-

R

-

K

-

V

-

I

-

E

S

I

V

F

F

T

T

D

E

A
x
T

L

N

I

V

-

Q

G

G

T

T

Q

Q

K

T

P

L

L

L

V

Q

I

C

T

A

S

V

G

D

V

A

G

G

I

V

L

G

R

R

S

V

E

V

K

H

A
x
V

I
x
S

T
|
T

E
x
D

D
x
E

-

V

-

Y

-

G

-

S

-

I

D

D

T

S

-

G

-

S

-

W

-

T

-

E

-

S

-

P

-

L

-

E

P

P

T

N

S

S

S

P

A
x
Y

S

A

V

A

P

S

R
x
K

A

A

A

G

S

S

E

D

E

L

A

V

A

A

L

R

E

A

A

Y

-

H

R

R

S

T

K

Y

G

G

V

L

N

D

A

V

Y

R

I

I

V

T

R

R

L

C

P

C

I
x
N

V
x
N

H

Y

G

G

K

P

D

Y

D

Q

H

H

-

P

-

E

G
x
K

F
x
L

I

I

P

P

I

L

V

F

I

V

G

T

M

N

D

L

K

L

E

D

N

G

G

G

Q

T

S

L

G
x
P

Y

L

I
x
Y

G

G

D

D

G

G

L

A

N

N

R

V

W
x
R

P

E

G

W

V

V

H

H

R

T

E

D

D

D

A

H

A

C

S

R

L

G

Y

I

R

A

L

L

I

V

V

L

E

A

K

G

Q

G

P

R

E

A

Q

G

V

E

V

I

F

Y

H

H

P

I

I

G

A

G

E

G

E

L

G

E

V

L

P

T

F
x
N

K

R

E

E

I

L

A

T

R

G

V

I

I

L

S

L

E

D

N

S

T

L

A

G

F

A

P

D

L

W

K

S

S

S

L

V

T

R

E

K

-

V

A

A

E

D

A
x
R

E

K

A

G

H
|
H

F

D

T

L

W

R

F

Y

T

S

H

L

F

D

A

G

G

G

M

-

D

-

A

-

Y

-

A

-

S

-

E

-

K

K

T

I

Q

E

N

R

I

E

L

L

G

G

W

Y

K

R

P

P

E

-

Q

Q

I

V

G

S

L

F

L

A

E

D

D

G

L

L

S

A

K

R

N

T

active site: T127 (= T121), D128 (≠ E122), E129 (≠ D123), Y151 (≠ A130), K155 (≠ R134)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ V12), D37 (≠ E26), S38 (≠ V27), L39 (= L28), T40 (≠ G29), G43 (≠ R32), D63 (= D51), I64 (= I52), F83 (≠ T71), A84 (= A72), A85 (≠ F73), S87 (≠ H75), T102 (≠ A97), V125 (≠ A119), S126 (≠ I120), Y151 (≠ A130), K155 (≠ R134), N181 (≠ V159)
binding thymidine-5'-diphosphate: H88 (≠ D76), E129 (≠ D123), N180 (≠ I158), K190 (≠ G166), L191 (≠ F167), P206 (≠ G182), Y208 (≠ I184), R215 (≠ W191), N250 (≠ F226), R274 (≠ A249), H277 (= H252)

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
21% identity, 99% coverage: 1:290/293 of query aligns to 1:306/322 of 1r6dA

query
sites
1r6dA

M

M

R

R

V

L

F

L

V

V

T

T

G

G

A

G

S

A

G
|
G

F
|
F

V
x
I

G

G

S

S

A

H

V

F

V

V

N

R

D

Q

L

L

I

L

A

A

A

G

G

A

-

Y

-

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

-

V

I

L

E
x
D

V
x
S

L
|
L

G
x
T

L

Y

V
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-

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x
Y

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-

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active site: T127 (≠ I107), N128 (≠ T108), Q129 (≠ S109), Y151 (≠ A130), K155 (≠ R134)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (≠ H75), H88 (≠ D76), T127 (≠ I107), N128 (≠ T108), Q129 (≠ S109), Y151 (≠ A130), N180 (≠ I158), K190 (≠ G166), L191 (≠ F167), P206 (≠ G182), Y208 (≠ I184), R215 (≠ W191), N250 (≠ F226), R274 (≠ A249), H277 (= H252), Y281 (≠ F256)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ V12), D37 (≠ E26), S38 (≠ V27), L39 (= L28), T40 (≠ G29), A42 (≠ V31), G43 (≠ R32), D63 (= D51), I64 (= I52), F83 (≠ T71), A84 (= A72), A85 (≠ F73), S87 (≠ H75), T102 (≠ E82), V125 (≠ L105), S126 (≠ V106), Y151 (≠ A130), K155 (≠ R134), N181 (≠ V159)

8fenA Panicum vigratum dihydroflavonol 4-reductase complexed with NADP and dhq (see paper)
31% identity, 40% coverage: 3:118/293 of query aligns to 7:135/326 of 8fenA

query
sites
8fenA

V

V

F

V

V

V

T

T

G

G

A

A

S

A

G
|
G

F
|
F

V
|
V

G

G

S

S

A

W

V

L

V

V

N

R

D

K

L

L

I

L

A

R

A

A

G

G

I

Y

E

A

V

V

L

R

G

A

L

T

V

V

R
|
R

S

D

Q

P

A

A

S

N

A

V

E

G

K
|
K

L

T

K

K

D

P

-

L

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D

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L

S

P

G

G

A

A

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A

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E

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R

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D

I
x
L

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A

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C

C

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H

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x
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T

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P

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M

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Y

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E

binding (2r,3r)-2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-2,3-dihydro-4h-chromen-4-one: T127 (≠ G110), A128 (≠ V111), G129 (= G112), N132 (≠ R115)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), F15 (= F11), V16 (= V12), R36 (= R32), K43 (= K39), D63 (= D51), L64 (≠ I52), V83 (≠ T71), A84 (= A72), T85 (≠ F73), T125 (= T108), S126 (= S109), T127 (≠ G110)

Sites not aligning to the query:

8femA Panicum vigratum dihydroflavonol 4-reductase complexed with NADP (see paper)
31% identity, 40% coverage: 3:118/293 of query aligns to 7:135/327 of 8femA

query
sites
8femA

V

V

F

V

V

V

T

T

G

G

A

A

S

A

G
|
G

F
|
F

V
|
V

G

G

S

S

A

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R

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A

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G

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A

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R
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R

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K
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K

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K

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P

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A

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A

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x
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x
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x
T

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A

G

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G

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T

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R

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S

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x
T

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S

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K

E

binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G10), F15 (= F11), V16 (= V12), R36 (= R32), K43 (= K39), D63 (= D51), L64 (≠ I52), V83 (≠ T71), A84 (= A72), T85 (≠ F73), T125 (= T108), S126 (= S109), T127 (≠ G110)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 162, 166, 189, 190, 192, 204

Query Sequence

>WP_035129577.1 NCBI__GCF_000769915.1:WP_035129577.1
MRVFVTGASGFVGSAVVNDLIAAGIEVLGLVRSQASAEKLKDSGAQILMGDINDPEIIRK
GATSCDAVIHTAFNHDFSRYKESCEDDRKVIEIFTDALIGTQKPLVITSGVGILRSEKAI
TEDDTPTSSASVPRAASEEAALEARSKGVNAYIVRLPIVHGKDDHGFIPIVIGMDKENGQ
SGYIGDGLNRWPGVHREDAASLYRLIVEKQPEQVVFHPIAEEGVPFKEIARVISENTAFP
LKSLTEAEAEAHFTWFTHFAGMDAYASSEKTQNILGWKPEQIGLLEDLSKNYF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory