SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035129644.1 NCBI__GCF_000769915.1:WP_035129644.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6z5uK Cryo-em structure of the a. Baumannii mlabdef complex bound to appnhp (see paper)
34% identity, 90% coverage: 1:240/267 of query aligns to 2:241/253 of 6z5uK

query
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6z5uK

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binding phosphoaminophosphonic acid-adenylate ester: R12 (≠ F11), S37 (= S36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (≠ V42), Q86 (= Q85), E164 (= E163), H197 (= H196)
binding magnesium ion: Q86 (= Q85), E164 (= E163)

7d0aB Acinetobacter mlafedb complex in adp-vanadate trapped vclose conformation (see paper)
34% identity, 90% coverage: 1:240/267 of query aligns to 4:243/263 of 7d0aB

query
sites
7d0aB

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binding adenosine-5'-diphosphate: S39 (= S36), T41 (≠ S38), G42 (= G39), K43 (= K40), E140 (= E137), S142 (= S139), M145 (= M142)

7d08B Acinetobacter mlafedb complex in atp-bound vtrans1 conformation (see paper)
34% identity, 90% coverage: 1:240/267 of query aligns to 4:243/263 of 7d08B

query
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7d08B

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binding adenosine-5'-triphosphate: R14 (≠ F11), R17 (≠ T14), G40 (= G37), T41 (≠ S38), K43 (= K40), T44 (= T41), T45 (≠ V42), E166 (= E163)

Q9AT00 Protein TRIGALACTOSYLDIACYLGLYCEROL 3, chloroplastic; ABC transporter I family member 13; ABC transporter ABCI.13; AtABCI13; Non-intrinsic ABC protein 11; AtNAP11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 90% coverage: 1:240/267 of query aligns to 84:338/345 of Q9AT00

query
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Q9AT00

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F94 (= F11) mutation to A: Reduced ATPase activity and transport properties.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 80% coverage: 1:214/267 of query aligns to 1:217/343 of P30750

query
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P30750

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40:46 (vs. 36:42, 57% identical) binding ATP
E166 (= E163) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
34% identity, 97% coverage: 1:260/267 of query aligns to 2:259/344 of 3tuzC

query
sites
3tuzC

M

M

I

I

E

K

I

L

K

S

D

N

I

I

E

T

K

K

S

V

F
|
F

G

H

D
x
Q

-

G

-

T

-

R

-

T

T

I

K

Q

V

A

L

L

K

N

G

N

I

V

S

S

T

L

T

H

F

V

E

P

T

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

R

A

S

S

G
|
G

S

A

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

I

K

R

T

C

L

V

L

N

G

L

I

L

H

E

T

R

P

P

E

T

K

E

G

G

S

S

I

V

S

L

F

V

D

D

G

G

R

Q

I

E

Y

L

S

T

E

T

L

L

T

S

R

E

D

S

E

E

K

L

R

T

S

K

L

A

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

N

T

V

V

E

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

K

E

M

L

F

-

T

D

N

N

K

T

R

P

G

K

R

D

E

E

I

V

K

K

D

R

R

R

V

V

N

T

E

E

V

L

L

L

E

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

N

H

Y

D

K

S

F

Y

P

P

N

S

E

N

I

L

S

S

G

G

G

G

M

Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

E

A

T

T

S

T

I

R

L

S

I

I

D

L

E

E

L

L

I

L

Q

K

E

D

I

I

T

N

R

R

E

R

Y

L

D

G

M

L

T

T

T

I

V

L

I

L

N

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

L

K

Q

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

N

N

G

G

-

E

I

L

K

I

E

E

W

Q

E

D

G

T

T

V

N

S

E

E

Q

V

I

F

L

S

E

H

T

P

R

K

N

T

K

P

S

L

I

A

V

Q

E

K

F

F

V

I

Y

Q

S

S

S

T

E

L

L

H

F

L

K

D

K

-

V

-

R

-

E

-

S

-

L

-

L

I

E

P

E

E

D

D

D

Y

E

Q

E

E

R

R

K

L

Q

Q

binding adenosine-5'-diphosphate: F12 (= F11), Q14 (≠ D13), G42 (= G37), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
34% identity, 97% coverage: 1:260/267 of query aligns to 2:259/344 of 3tuiC

query
sites
3tuiC

M

M

I

I

E

K

I

L

K

S

D

N

I

I

E

T

K

K

S

V

F
|
F

G

H

D

Q

-

G

-

T

-

R

-

T

T

I

K

Q

V

A

L

L

K

N

G

N

I

V

S

S

T

L

T

H

F

V

E

P

T

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

R

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

I

K

R

T

C

L

V

L

N

G

L

I

L

H

E

T

R

P

P

E

T

K

E

G

G

S

S

I

V

S

L

F

V

D

D

G

G

R

Q

I

E

Y

L

S

T

E

T

L

L

T

S

R

E

D

S

E

E

K

L

R

T

S

K

L

A

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

N

T

V

V

E

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

K

E

M

L

F

-

T

D

N

N

K

T

R

P

G

K

R

D

E

E

I

V

K

K

D

R

R

R

V

V

N

T

E

E

V

L

L

L

E

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

N

H

Y

D

K

S

F

Y

P

P

N

S

E

N

I

L

S

S

G

G

G

G

M

Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

E

A

T

T

S

T

I

R

L

S

I

I

D

L

E

E

L

L

I

L

Q

K

E

D

I

I

T

N

R

R

E

R

Y

L

D

G

M

L

T

T

T

I

V

L

I

L

N

I

T

T

H

H

D

E

M

M

N

D

S

V

V

V

L

K

Q

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

N

N

G

G

-

E

I

L

K

I

E

E

W

Q

E

D

G

T

T

V

N

S

E

E

Q

V

I

F

L

S

E

H

T

P

R

K

N

T

K

P

S

L

I

A

V

Q

E

K

F

F

V

I

Y

Q

S

S

S

T

E

L

L

H

F

L

K

D

K

-

V

-

R

-

E

-

S

-

L

-

L

I

E

P

E

E

D

D

D

Y

E

Q

E

E

R

R

K

L

Q

Q

binding adenosine-5'-diphosphate: F12 (= F11), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
34% identity, 97% coverage: 1:260/267 of query aligns to 2:259/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

E

K

I

L

K

S

D

N

I

I

E

T

K

K

S

V

F
|
F

G

H

D
x
Q

-

G

-

T

-

R

-

T

T
x
I

K

Q

V

A

L

L

K

N

G

N

I

V

S

S

T

L

T

H

F

V

E

P

T

A

G

G

K

Q

T

I

N

Y

L

G

I

V

I

I

G

G

R

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

I

K

R

T

C

L

V

L

N

G

L

I

L

H

E

T

R

P

P

E

T

K

E

G

G

S

S

I

V

S

L

F

V

D

D

G

G

R

Q

I

E

Y

L

S

T

E

T

L

L

T

S

R

E

D

S

E

E

K

L

R

T

S

K

L

A

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

G

H

S

F

A

N

L

L

F

L

D

S

S

S

M

R

N

T

V

V

E

F

D

G

N

N

V

V

A

A

F

L

P

P

L

L

K

E

M

L

F

-

T

D

N

N

K

T

R

P

G

K

R

D

E

E

I

V

K

K

D

R

R

R

V

V

N

T

E

E

V

L

L

L

E

S

R

L

V

V

N

G

L

L

V

G

D

D

A

K

N

H

Y

D

K

S

F

Y

P

P

N

S

E
x
N

I

L

S
|
S

G

G

G

G

M
x
Q

Q

K

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

S

N

N

P

P

K

K

Y

V

L

L

F

L

C

C

D

D

E

Q

P

A

N

T

S

S

G

A

L

L

D

D

P

P

E

A

T

T

S

T

I

R

L

S

I

I

D

L

E

E

L

L

I

L

Q

K

E

D

I

I

T

N

R

R

E

R

Y

L

D

G

M

L

T

T

T

I

V

L

I

L

N

I

T

T

H
|
H

D

E

M

M

N

D

S

V

V

V

L

K

Q

R

I

I

G

C

E

D

K

C

I

V

V

A

F

V

L

I

K

S

N

N

G

G

-

E

I

L

K

I

E

E

W

Q

E

D

G

T

T

V

N

S

E

E

Q

V

I

F

L

S

E

H

T

P

R

K

N

T

K

P

S

L

I

A

V

Q

E

K

F

F

V

I

Y

Q

S

S

S

T

E

L

L

H

F

L

K

D

K

-

V

-

R

-

E

-

S

-

L

-

L

I

E

P

E

E

D

D

D

Y

E

Q

E

E

R

R

K

L

Q

Q

binding phosphothiophosphoric acid-adenylate ester: F12 (= F11), Q14 (≠ D13), I19 (≠ T14), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (≠ T41), T47 (≠ V42), N141 (≠ E137), S143 (= S139), Q146 (≠ M142), H200 (= H196)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
36% identity, 89% coverage: 1:237/267 of query aligns to 2:239/241 of 4u00A

query
sites
4u00A

M

I

I

I

E

R

I

I

K

R

D

N

I

L

E

H

K

K

S

W

F
|
F

G

G

D

P

T

L

K

H

V
|
V

L

L

K

K

G

G

I

I

S

H

T

L

T

E

F

V

E

A

T

P

G

G

K

E

T

K

N

L

L

V

I

I

I

I

G

G

R

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

I

K

R

T

T

L

I

L

N

G

R

I

L

H

E

T

D

P

F

E

Q

K

E

G

G

S

E

I

V

S

V

F

V

D

D

G

G

R

L

I

S

Y

-

S

-

E

-

L

-

T

V

R

K

D

D

E

D

K

R

-

A

-

L

R

R

S

E

L

I

R

R

T

R

E

E

I

V

G

G

M

M

V

V

F

F

Q

Q

G

Q

S

F

A

N

L

L

F

F

D

P

S

H

M

M

N

T

V

V

E

L

D

E

N

N

V

V

A

T

F

L

-

A

P

P

L

M

K

R

M

V

F

R

T

R

N

W

K

P

R

R

G

E

R

K

E

A

I

E

K

K

D

-

R

K

V

A

N

L

E

E

V

L

L

L

E

E

R

R

V

V

N

G

L

I

V

L

D

D

A

Q

N

A

Y

R

K

K

F

Y

P

P

N

A

E

Q

I

L

S

S

G

G

G

G

M

Q

Q

Q

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

E

E

T

M

S

V

I

G

L

E

I

V

D

L

E

D

L

V

I

M

Q

R

E

D

I

L

T

A

R

-

E

Q

Y

G

D

G

M

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

L

R

Q

E

I

V

G

A

E

D

K

R

I

V

V

V

F

F

L

M

K

D

N

G

G

G

I

Q

K

I

E

V

W

E

E

E

G

G

T

R

N

P

E

E

Q

E

I

I

L

F

-

T

-

R

-

P

-

K

E

E

T

E

R

R

N

T

K

R

S

S

I

F

V

L

E

Q

F

R

V

V

binding adenosine-5'-diphosphate: F12 (= F11), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (≠ V42)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
37% identity, 80% coverage: 1:214/267 of query aligns to 1:213/219 of 8w6iD

query
sites
8w6iD

M

M

I

I

E

R

I

F

K

E

D

H

I

V

E

S

K

K

S

A

F
x
Y

-

L

G

G

D

G

T

R

K

Q

V

A

L

L

K

Q

G

G

I

V

S

T

T

F

T

H

F

M

E

Q

T

P

G

G

K

E

T

M

N

A

L

F

I

L

I

T

G

G

R

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

L

K

K

T

L

L

I

L

C

G

G

I

I

H

E

T

R

P

P

E

S

K

A

G

G

S

K

I

I

S

W

F

F

D

S

G

G

R

H

I

D

Y

I

S

T

E

R

L

L

T

K

R

N

D

R

E

E

K

V

R

P

S

F

L

L

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q
|
Q

G

D

S

H

A

H

L

L

F

L

D

M

S

D

M

R

N

T

V

V

E

Y

D

D

N

N

V

V

A

A

F

I

P

P

L

L

K

-

M

I

F

I

T

A

N

G

K

A

R

S

G

G

R

D

E

D

I

I

K

R

D

R

R

R

V

V

N

S

E

A

V

A

L

L

E

D

R

K

V

V

N

G

L

L

V

L

D

D

A
x
K

N

A

Y

K

K

N

F

F

P

P

N

I

E
x
Q

I

L

S
|
S

G
|
G

G
|
G

M
x
E

Q

Q

K

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

N

K

P

P

K

A

Y

V

L

L

F

L

C

A

D

D

E

E

P

P

N

T

S

G

G

N

L

L

D

D

P

D

E

A

T

L

S

S

I

E

L

G

I

I

D

L

E

R

L

L

I

F

Q

E

E

E

I

F

T

N

R

R

E

-

Y

V

D

G

M

V

T

T

T

V

V

L

I

M

N

A

T

T

H
|
H

D

D

M

I

N

N

S

L

V

I

L

S

Q

R

I

R

G

S

E

Y

K

R

I

M

V

L

F

T

L

L

K

S

N

D

G

G

binding phosphothiophosphoric acid-adenylate ester: Y11 (≠ F11), S37 (= S36), G38 (= G37), A39 (≠ S38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (≠ V42), Q86 (= Q85), K130 (≠ A130), Q137 (≠ E137), S139 (= S139), G140 (= G140), G141 (= G141), E142 (≠ M142), H195 (= H196)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
37% identity, 80% coverage: 1:214/267 of query aligns to 1:213/222 of P0A9R7

query
sites
P0A9R7

M

M

I

I

E

R

I

F

K

E

D

H

I

V

E

S

K

K

S

A

F

Y

-

L

G

G

D

G

T

R

K

Q

V

A

L

L

K

Q

G

G

I

V

S

T

T

F

T

H

F

M

E

Q

T

P

G

G

K

E

T

M

N

A

L

F

I

L

I

T

G

G

R

H

S

S

G

G

S

A

G

G

K
|
K

T

S

V

T

M

L

L

L

K

K

T

L

L

I

L
x
C

G

G

I

I

H

E

T

R

P

P

E

S

K

A

G

G

S

K

I

I

S

W

F

F

D

S

G

G

R

H

I

D

Y

I

S

T

E

R

L

L

T

K

R

N

D

R

E

E

K

V

R

P

S

F

L

L

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

G

D

S

H

A

H

L

L

F

L

D

M

S

D

M

R

N

T

V

V

E

Y

D

D

N

N

V

V

A

A

F

I

P

P

L

L

K

-

M

I

F

I

T

A

N

G

K

A

R

S

G

G

R

D

E

D

I

I

K

R

D

R

R

R

V

V

N

S

E

A

V

A

L

L

E

D

R

K

V

V

N

G

L

L

V

L

D

D

A

K

N

A

Y

K

K

N

F

F

P

P

N

I

E

Q

I

L

S

S

G

G

G

G

M

E

Q

Q

K

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

N

K

P

P

K

A

Y

V

L

L

F

L

C

A

D

D

E

E

P

P

N

T

S

G

G

N

L

L

D

D

P

D

E

A

T

L

S

S

I

E

L

G

I

I

D

L

E

R

L

L

I

F

Q

E

E

E

I

F

T

N

R

R

E

-

Y

V

D

G

M

V

T

T

T

V

V

L

I

M

N

A

T

T

H

H

D

D

M

I

N

N

S

L

V

I

L

S

Q

R

I

R

G

S

E

Y

K

R

I

M

V

L

F

T

L

L

K

S

N

D

G

G

K41 (= K40) mutation to R: Does not bind ATP.
C49 (≠ L48) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
36% identity, 80% coverage: 1:214/267 of query aligns to 1:213/218 of 8hd0A

query
sites
8hd0A

M

M

I

I

E

R

I

F

K

E

D

H

I

V

E

S

K

K

S

A

F
x
Y

-

L

G

G

D

G

T
x
R

K

Q

V
x
A

L

L

K

Q

G

G

I

V

S

T

T

F

T

H

F

M

E

Q

T

P

G

G

K

E

T

M

N

A

L

F

I

L

I

T

G

G

R

H

S

S

G

G

S

A

G
|
G

K
|
K

T
x
S

V
x
T

M

L

L

L

K

K

T

L

L

I

L

C

G

G

I

I

H

E

T

R

P

P

E

S

K

A

G

G

S

K

I

I

S

W

F

F

D

S

G

G

R

H

I

D

Y

I

S

T

E

R

L

L

T

K

R

N

D

R

E

E

K

V

R

P

S

F

L

L

R

R

T

R

E

Q

I

I

G

G

M

M

V

I

F

F

Q

Q

G

D

S

H

A

H

L

L

F

L

D

M

S

D

M

R

N

T

V

V

E

Y

D

D

N

N

V

V

A

A

F

I

P

P

L

L

K

-

M

I

F

I

T

A

N

G

K

A

R

S

G

G

R

D

E

D

I

I

K

R

D

R

R

R

V

V

N

S

E

A

V

A

L

L

E

D

R

K

V

V

N

G

L

L

V

L

D

D

A

K

N

A

Y

K

K

N

F

F

P

P

N

I

E

Q

I

L

S
|
S

G

G

G
|
G

M

E

Q

Q

K

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

I

V

V

V

N

N

N

K

P

P

K

A

Y

V

L

L

F

L

C

A

D

D

E

Q

P

P

N

T

S

G

G

N

L

L

D

D

P

D

E

A

T

L

S

S

I

E

L

G

I

I

D

L

E

R

L

L

I

F

Q

E

E

E

I

F

T

N

R

R

E

-

Y

V

D

G

M

V

T

T

T

V

V

L

I

M

N

A

T

T

H

H

D

D

M

I

N

N

S

L

V

I

L

S

Q

R

I

R

G

S

E

Y

K

R

I

M

V

L

F

T

L

L

K

S

N

D

G

G

binding adenosine-5'-triphosphate: Y11 (≠ F11), R15 (≠ T14), A17 (≠ V16), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (≠ V42), S139 (= S139), G141 (= G141)

1g291 Malk (see paper)
31% identity, 90% coverage: 2:242/267 of query aligns to 4:245/372 of 1g291

query
sites
1g291

I

V

E

R

I

L

K

V

D

D

I

V

E

W

K

K

S

V

F

F

G

G

D

E

T

V

K

T

V

A

L

V

K

R

G

E

I

M

S

S

T

L

T

E

F

V

E

K

T

D

G

G

K

E

T

F

N

M

L

I

I

L

I

L

G

G

R

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

V
x
T

M

T

L

L

K

R

T

M

L

I

L

A

G

G

I

L

H

E

T

E

P

P

E

S

K

R

G

G

S

Q

I

I

S

Y

F

-

D

-

G

-

R

-

I

I

Y

G

S

D

E

K

L

L

T

V

R

A

D
|
D

E

P

K
x
E

R
x
K

S

G

L

I

R

F

T

V

-

P

-

P

-
x
K

-
x
D

-

R

E

D

I

I

G

A

M

M

V

V

F

F

Q

Q

G

S

S

Y

A

A

L

L

F

Y

D

P

S

H

M

M

N

T

V

V

E

Y

D

D

N

N

V

I

A

A

F

F

P

P

L

L

K

K

M

L

F

R

T

K

N

V

K

P

R

R

G

-

R

Q

E

E

I

I

K

D

D

Q

R

R

V

V

N

R

E

E

V

V

L

A

E

E

R

L

V

L

N

G

L

L

V

T

D

E

A

L

N

L

Y

N

K

R

F

K

P

P

N

R

E

E

I

L

S

S

G

G

G

G

M

Q

Q

R

K

Q

R

R

V

V

A

A

I

L

A

G

R

R

A

A

I

I

V

V

N

R

N

K

P

P

K

Q

Y

V

L

F

F

L

C

M

D

D

E

E

P

P

N

L

S

S

G

N

L

L

D

D

P

A

E

K

T

L

S

R

I

V

L

R

I

M

D

R

E

A

L

E

I

L

Q

K

E

K

I

L

T

Q

R

R

E

Q

Y

L

D

G

M

V

T

T

T

T

V

I

I

Y

N

V

T

T

H

H

D

D

M

Q

N

V

S

E

V

A

L

M

Q

T

I

M

G

G

E

D

K

R

I

I

V

A

F

V

L

M

K

N

N

R

G

G

I

V

K

L

E

Q

W

Q

E

V

G

G

T

S

N

P

E

D

Q

E

I

V

L

Y

E

D

-

K

T

P

R

A

N

N

K

T

S

F

I

V

V

A

E

G

F

F

V

I

Y

G

S

S

S

P

E

P

L

M

binding magnesium ion: D69 (= D71), E71 (≠ K73), K72 (≠ R74), K79 (vs. gap), D80 (vs. gap)
binding pyrophosphate 2-: S38 (= S36), G39 (= G37), C40 (≠ S38), G41 (= G39), K42 (= K40), T43 (= T41), T44 (≠ V42)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

3c4jA Abc protein artp in complex with atp-gamma-s
34% identity, 89% coverage: 1:238/267 of query aligns to 3:242/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

E

K

K

K

S

S

F
|
F

G

G

D

S

T

L

K

E

V
|
V

L

L

K

K

G

G

I

I

S

N

T

V

T

H

F

I

E

R

T

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

R

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

M

F

L

L

K

R

T

C

L

L

L

N

G

L

I

L

H

E

T

D

P

F

E

D

K

E

G

G

S

E

I

I

S

I

F

I

D

D

G

G

R

-

I

I

Y

N

S

L

E

K

L

A

T

K

R

D

D

T

E

N

K

L

R

N

S

K

L

V

R

R

T

E

E

E

I

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

N

T

V

V

E

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

K

K

M

V

F

R

T

K

N

W

K

P

R

R

G

-

R

E

E

K

I

A

K

E

D

A

R

K

V

A

N

M

E

E

V

L

L

L

E

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

N

A

Y

H

K

A

F

Y

P

P

N

D

E

S

I

L

S

S

G

G

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

E

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

Q

K

E

Q

I

L

T

A

R

N

E

E

Y

-

D

G

M

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

L

R

Q

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

N

G

G

G

I

Y

K

I

E

I

W

E

E

E

G

G

T

K

N

P

E

E

Q

D

I

L

L

F

E

D

T

R

-

P

-

Q

-

H

-

E

R

R

N

T

K

K

S

A

I

F

V

L

E

S

F

K

V

V

Y

F

binding phosphothiophosphoric acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

3c41J Abc protein artp in complex with amp-pnp/mg2+
34% identity, 89% coverage: 1:238/267 of query aligns to 3:242/242 of 3c41J

query
sites
3c41J

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

E

K

K

K

S

S

F
|
F

G

G

D

S

T

L

K

E

V
|
V

L

L

K

K

G

G

I

I

S

N

T

V

T

H

F

I

E

R

T

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

R

P

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

V
x
T

M

F

L

L

K

R

T

C

L

L

L

N

G

L

I

L

H

E

T

D

P

F

E

D

K

E

G

G

S

E

I

I

S

I

F

I

D

D

G

G

R

-

I

I

Y

N

S

L

E

K

L

A

T

K

R

D

D

T

E

N

K

L

R

N

S

K

L

V

R

R

T

E

E

E

I

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

N

T

V

V

E

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

K

K

M

V

F

R

T

K

N

W

K

P

R

R

G

-

R

E

E

K

I

A

K

E

D

A

R

K

V

A

N

M

E

E

V

L

L

L

E

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

N

A

Y

H

K

A

F

Y

P

P

N

D

E

S

I

L

S

S

G

G

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D
|
D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

E

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

Q

K

E

Q

I

L

T

A

R

N

E

E

Y

-

D

G

M

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

L

R

Q

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

N

G

G

G

I

Y

K

I

E

I

W

E

E

E

G

G

T

K

N

P

E

E

Q

D

I

L

L

F

E

D

T

R

-

P

-

Q

-

H

-

E

R

R

N

T

K

K

S

A

I

F

V

L

E

S

F

K

V

V

Y

F

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)
binding magnesium ion: S43 (≠ T41), D163 (= D162)

2olkA Abc protein artp in complex with adp-beta-s
34% identity, 89% coverage: 1:238/267 of query aligns to 3:242/242 of 2olkA

query
sites
2olkA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

E

K

K

K

S

S

F
|
F

G

G

D

S

T

L

K

E

V
|
V

L

L

K

K

G

G

I

I

S

N

T

V

T

H

F

I

E

R

T

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

R

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

M

F

L

L

K

R

T

C

L

L

L

N

G

L

I

L

H

E

T

D

P

F

E

D

K

E

G

G

S

E

I

I

S

I

F

I

D

D

G

G

R

-

I

I

Y

N

S

L

E

K

L

A

T

K

R

D

D

T

E

N

K

L

R

N

S

K

L

V

R

R

T

E

E

E

I

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

N

T

V

V

E

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

K

K

M

V

F

R

T

K

N

W

K

P

R

R

G

-

R

E

E

K

I

A

K

E

D

A

R

K

V

A

N

M

E

E

V

L

L

L

E

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

N

A

Y

H

K

A

F

Y

P

P

N

D

E

S

I

L

S

S

G

G

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

E

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

Q

K

E

Q

I

L

T

A

R

N

E

E

Y

-

D

G

M

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

L

R

Q

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

N

G

G

G

I

Y

K

I

E

I

W

E

E

E

G

G

T

K

N

P

E

E

Q

D

I

L

L

F

E

D

T

R

-

P

-

Q

-

H

-

E

R

R

N

T

K

K

S

A

I

F

V

L

E

S

F

K

V

V

Y

F

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

2oljA Abc protein artp in complex with adp/mg2+
34% identity, 89% coverage: 1:238/267 of query aligns to 3:242/242 of 2oljA

query
sites
2oljA

M

M

I

I

E

D

I

V

K

H

D

Q

I

L

E

K

K

K

S

S

F
|
F

G

G

D

S

T

L

K

E

V
|
V

L

L

K

K

G

G

I

I

S

N

T

V

T

H

F

I

E

R

T

E

G

G

K

E

T

V

N

V

L

V

I

V

I

I

G

G

R

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

V
x
T

M

F

L

L

K

R

T

C

L

L

L

N

G

L

I

L

H

E

T

D

P

F

E

D

K

E

G

G

S

E

I

I

S

I

F

I

D

D

G

G

R

-

I

I

Y

N

S

L

E

K

L

A

T

K

R

D

D

T

E

N

K

L

R

N

S

K

L

V

R

R

T

E

E

E

I

V

G

G

M

M

V

V

F

F

Q

Q

G

R

S

F

A

N

L

L

F

F

D

P

S

H

M

M

N

T

V

V

E

L

D

N

N

N

V

I

A

T

F

L

-

A

P

P

L

M

K

K

M

V

F

R

T

K

N

W

K

P

R

R

G

-

R

E

E

K

I

A

K

E

D

A

R

K

V

A

N

M

E

E

V

L

L

L

E

D

R

K

V

V

N

G

L

L

V

K

D

D

A

K

N

A

Y

H

K

A

F

Y

P

P

N

D

E

S

I

L

S

S

G

G

G

G

M

Q

Q

A

K

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

I

L

V

A

N

M

N

E

P

P

K

K

Y

I

L

M

F

L

C

F

D

D

E

E

P

P

N

T

S

S

G

A

L

L

D

D

P

P

E

E

T

M

S

V

I

G

L

E

I

V

D

L

E

S

L

V

I

M

Q

K

E

Q

I

L

T

A

R

N

E

E

Y

-

D

G

M

M

T

T

T

M

V

V

I

V

N

V

T

T

H

H

D

E

M

M

N

G

S

F

V

A

L

R

Q

E

I

V

G

G

E

D

K

R

I

V

V

L

F

F

L

M

K

D

N

G

G

G

I

Y

K

I

E

I

W

E

E

E

G

G

T

K

N

P

E

E

Q

D

I

L

L

F

E

D

T

R

-

P

-

Q

-

H

-

E

R

R

N

T

K

K

S

A

I

F

V

L

E

S

F

K

V

V

Y

F

binding adenosine-5'-diphosphate: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (≠ T41), T44 (≠ V42)

7chaI Cryo-em structure of p.Aeruginosa mlafebd with amppnp (see paper)
33% identity, 88% coverage: 2:237/267 of query aligns to 4:239/262 of 7chaI

query
sites
7chaI

I

V

E

E

I

L

K

K

D

G

I

L

E

T

K

F

S

K

F
x
R

G

G

D

S

T

R

K

A

V

I

L

F

K

D

G

N

I

I

S

D

T

V

T

R

F

I

E

P

T

R

G

G

K

K

T

V

N

T

L

G

I

I

I

M

G

G

R

P

S
|
S

G
|
G

S
x
C

G
|
G

K
|
K

T
|
T

V
x
T

M

L

L

L

K

R

T

L

L

I

L

A

G

S

I

Q

H

L

T

R

P

P

E

S

K

K

G

G

S

E

I

V

S

W

F

V

D

N

G

G

R

Q

I

N

Y

L

S

P

E

Q

L

L

T

S

R

R

D

G

E

D

K

L

R

F

S

D

L

M

R

R

T

K

E

Q

I

F

G

G

M

V

V

L

F

F

Q

Q

G

S

S

G

A

A

L

L

F

F

D

T

S

D

M

L

N

D

V

V

E

F

D

E

N

N

V

V

A

A

F

F

P

P

L

L

K

R

M

V

F

H

T

T

N

Q

K

L

R

P

G

E

R

E

E

M

I

I

K

R

D

D

R

I

V

V

N

L

E

M

V

K

L

L

E

Q

R

A

V

V

N

G

L

L

V

R

D

G

A

A

N

V

Y

E

K

L

F

M

P

P

N

D

E

E

I

L

S

S

G

G

G

G

M

M

Q

K

K

R

R

R

V

V

A

A

I

L

A

A

R

R

A

A

I

I

V

A

N

L

N

D

P

P

K

Q

Y

I

L

L

F

L

C

Y

D

D

E

E

P

P

N

F

S

V

G

G

L

Q

D

D

P

P

E

I

T

A

S

M

I

G

L

V

I

L

D

V

E

R

L

L

I

I

Q

R

E

L

I

L

T

N

R

D

E

A

Y

L

D

G

M

I

T

T

T

S

V

I

I

V

N

V

T

S

H

H

D

D

M

L

N

A

S

E

V

T

L

A

Q

S

I

I

G

A

E

D

K

Y

I

I

V

Y

F

I

L

V

K

G

N

D

G

G

I

R

K

V

E

L

W

G

E

H

G

G

T

T

N

P

E

D

Q

V

I

L

L

K

E

E

T

T

R

D

N

D

K

P

S

R

I

I

V

R

E

Q

F

F

V

V

binding phosphoaminophosphonic acid-adenylate ester: R13 (≠ F11), S38 (= S36), G39 (= G37), C40 (≠ S38), G41 (= G39), K42 (= K40), T43 (= T41), T44 (≠ V42)

6z4wA Ftse structure from streptococcus pneumoniae in complex with adp (space group p 1) (see paper)
37% identity, 80% coverage: 1:214/267 of query aligns to 3:215/230 of 6z4wA

query
sites
6z4wA

M

I

I

I

E

E

I

M

K

R

D

D

I

V

E

V

K

K

S

K

F
x
Y

G

D

D

N

-

G

T
|
T

K

T

V
x
A

L

L

K

R

G

G

I

V

S

S

T

V

T

S

F

V

E

Q

T

P

G

G

K

E

T

F

N

A

L

Y

I

I

I

V

G

G

R

P

S

S

G
|
G

S

A

G
|
G

K
|
K

T
x
S

V
x
T

M

F

L

I

K

R

T

S

L

L

L

Y

G

R

I

E

H

V

T

K

P

I

E

D

K

K

G

G

S

S

I

L

S

S

F

V

D

A

G

G

R

F

I

N

Y

L

S

V

E

K

L

I

T

K

R

K

D

K

E

D

K

V

R

P

S

L

L

L

R

R

T

R

E

S

I

V

G

G

M

V

V

V

F

F

Q

Q

G

D

S

Y

A

K

L

L

F

L

D

P

S

K

M

K

N

T

V

V

E

Y

D

E

N

N

V

I

A

A

F

Y

P

A

L

M

K

E

M

V

F

I

T

G

N

E

K

N

R

R

G

-

R

R

E

N

I

I

K

K

D

R

R

R

V

V

N

M

E

E

V

V

L

L

E

D

R

L

V

V

N

G

L

L

V

K