SitesBLAST
Comparing WP_035131390.1 NCBI__GCF_000769915.1:WP_035131390.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
33% identity, 83% coverage: 61:448/470 of query aligns to 16:420/425 of O59010
- S65 (≠ T104) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ T310) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ TSS 310:312) binding L-aspartate
- M311 (≠ L345) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T348) binding L-aspartate
- V355 (≠ I389) binding L-aspartate
- D394 (= D422) binding L-aspartate
- M395 (= M423) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R425) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N429) binding L-aspartate
- D405 (≠ N433) mutation to N: Strongly decreased affinity for aspartate.
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
34% identity, 78% coverage: 86:450/470 of query aligns to 42:419/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ K230), G195 (≠ I234), R282 (≠ E321)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ T310), S272 (= S311), S273 (= S312), M307 (≠ L345), T310 (= T348), G353 (≠ R391), A354 (= A392), R394 (= R425), T395 (= T426)
Sites not aligning to the query:
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
34% identity, 78% coverage: 86:450/470 of query aligns to 43:420/425 of 6zgbA
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
34% identity, 78% coverage: 86:450/470 of query aligns to 45:422/427 of 5e9sA
- binding aspartic acid: R274 (≠ T310), S275 (= S311), S276 (= S312), T313 (= T348), G353 (= G388), V354 (≠ I389), A357 (= A392), G358 (≠ S393), D394 (= D422), R397 (= R425), T398 (= T426)
- binding decyl-beta-d-maltopyranoside: L194 (≠ K230), G198 (≠ I234), Y202 (≠ F238)
- binding sodium ion: Y87 (≠ F129), T90 (≠ V132), S91 (≠ T133), S276 (= S312), G305 (= G340), A306 (≠ Y341), T307 (≠ S342), N309 (= N344), N309 (= N344), M310 (≠ L345), D311 (= D346), S348 (= S383), I349 (≠ K384), G350 (= G385), T351 (≠ V386), N401 (= N429), V402 (= V430), D405 (≠ N433)
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
34% identity, 78% coverage: 86:450/470 of query aligns to 45:422/426 of 6xwnB
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
33% identity, 82% coverage: 61:447/470 of query aligns to 16:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L85), F46 (≠ L85), P75 (≠ N114), L91 (≠ I131), F95 (≠ L135), L130 (≠ W171), I133 (= I174), I159 (≠ V200), Y167 (≠ M208), K196 (= K230), G200 (≠ I234), I207 (= I241), F210 (≠ G244), L250 (≠ I284), I262 (= I296), M269 (≠ V303), T334 (≠ S368), V335 (≠ I369), G336 (= G370), T340 (≠ I374), L343 (≠ F377), M399 (≠ S427)
- binding aspartic acid: S277 (= S311), S278 (= S312), T314 (= T348), G354 (= G388), A358 (= A392), G359 (≠ S393), D394 (= D422), R397 (= R425), T398 (= T426)
- binding sodium ion: Y89 (≠ F129), T92 (≠ V132), S93 (≠ T133), G306 (= G340), T308 (≠ S342), N310 (= N344), N310 (= N344), M311 (≠ L345), D312 (= D346), S349 (= S383), I350 (≠ K384), T352 (≠ V386), N401 (= N429), V402 (= V430), D405 (≠ N433)
Sites not aligning to the query:
2nwwA Crystal structure of gltph in complex with tboa (see paper)
33% identity, 82% coverage: 61:444/470 of query aligns to 7:407/407 of 2nwwA
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
32% identity, 82% coverage: 61:445/470 of query aligns to 8:409/409 of 6bavA
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
33% identity, 82% coverage: 61:444/470 of query aligns to 13:413/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G108), V83 (≠ L126), I157 (= I201), Y164 (≠ M208), K193 (= K230), T305 (≠ S342), I306 (≠ F343), I347 (≠ K384)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (= I61), M199 (= M236), S275 (= S312), T311 (= T348), G356 (≠ S393), L384 (= L415), D391 (= D422), R394 (= R425)
Sites not aligning to the query:
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
34% identity, 77% coverage: 86:448/470 of query aligns to 38:409/416 of 6r7rA
- binding d-aspartic acid: R263 (≠ T310), S265 (= S312), M299 (≠ L345), T302 (= T348), T340 (≠ V386), G342 (= G388), V343 (≠ I389), G347 (≠ S393), D383 (= D422), R386 (= R425), T387 (= T426), N390 (= N429)
- binding decyl-beta-d-maltopyranoside: V212 (≠ L259), A216 (≠ L263)
Sites not aligning to the query:
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
32% identity, 82% coverage: 61:444/470 of query aligns to 8:408/408 of 6bauA
- binding cysteine: S270 (= S312), M303 (≠ L345), T306 (= T348), A345 (= A387), G346 (= G388), V347 (≠ I389), G351 (≠ S393), D386 (= D422), C389 (≠ R425), T390 (= T426), N393 (= N429)
6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
31% identity, 82% coverage: 61:444/470 of query aligns to 8:396/396 of 6bmiA