SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035132405.1 NCBI__GCF_000769915.1:WP_035132405.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
55% identity, 95% coverage: 5:226/233 of query aligns to 3:224/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G47), K46 (= K48), S47 (= S49)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
52% identity, 95% coverage: 5:225/233 of query aligns to 3:223/615 of 5lilA

query
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5lilA

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binding adenosine-5'-triphosphate: F13 (= F15), V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R138), Q143 (= Q145), S145 (= S147), G147 (= G149), Q148 (= Q150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
52% identity, 95% coverage: 5:225/233 of query aligns to 3:223/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: V22 (= V24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (= S49), T48 (= T50), Q92 (= Q94), K136 (≠ R138), Q143 (= Q145), S145 (= S147), G147 (= G149), Q148 (= Q150)
binding magnesium ion: S47 (= S49), Q92 (= Q94)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
50% identity, 97% coverage: 3:228/233 of query aligns to 2:227/650 of 5ws4A

query
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5ws4A

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binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (= S49)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
52% identity, 94% coverage: 4:222/233 of query aligns to 3:221/648 of P75831

query
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P75831

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K47 (= K48) mutation to L: Lack of activity.
D169 (= D170) mutation to N: Lack of activity.

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
49% identity, 95% coverage: 5:225/233 of query aligns to 1:226/232 of 1f3oA

query
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1f3oA

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T

Q

F

F

N

N

L

L

L

I

P

P

R

L

T

L

T

T

A

A

L

L

D

E

N

N

V

V

A

E

L

L

P

P

M

L

V

I

Y

F

-

K

-

Y

-

R

A

G

G

A

Y

M

K

S

K

G

P

E

E

E

R

R

N

R

E

K

R

R

A

A

S

L

Q

E

V

C

L

L

T

K

Q

M

V

A

G

E

L

L

E

E

D

E

R

R

M

F

-

A

D

N

H

H

K

K

P

P

N

N

Q

Q

L

L

S

S

G

G

G

G

Q

Q

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

A

N

N

H

N

P

P

S

P

I

I

I

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

T

S

G

V

E

E

K

I

I

M

M

K

Q

L

L

F

L

N

K

E

K

I

L

H

N

-

E

A

E

N

D

G

G

N

K

T

T

V

V

I

V

L

V

V

V

T

T

H

H

E

D

E

I

D

N

I

V

A

A

A

R

Y

F

A

G

H

E

R

R

V

I

I

I

R

Y

L

L

R

K

D

D

G

G

V

E

I

V

E

E

S

R

D

E

binding adenosine-5'-diphosphate: Y11 (≠ F15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
48% identity, 95% coverage: 5:225/233 of query aligns to 1:226/230 of 1l2tA

query
sites
1l2tA

I

M

I

I

D

K

I

L

K

K

S

N

I

V

T

T

R

K

N

T

F
x
Y

P

K

L

M

G

G

N

E

E

E

V

I

V

I

K

Y

V

A

L

L

K

K

G

N

I

V

D

N

L

L

T

N

I

I

N

K

K

E

G

G

E

E

Y

F

V

V

A

S

L

I

M

M

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

M

L

N

N

L

I

L

I

G

G

C

C

L

L

D

D

T

K

P

P

T

T

G

E

G

G

T

E

Y

V

I

Y

L

I

N

D

G

N

K

I

D

K

V

T

S

N

K

D

M

L

S

D

D

D

N

D

E

E

L

L

A

T

E

K

I

I

R

R

N

R

K

D

E

K

I

I

G

G

F

F

V

V

F

F

Q

Q

T

Q

F

F

N

N

L

L

L

I

P

P

R

L

T

L

T

T

A

A

L

L

D

E

N

N

V

V

A

E

L

L

P

P

M

L

V

I

Y

F

-

K

-

Y

-

R

A

G

G

A

Y

M

K

S

K

G

P

E

E

E

R

R

N

R

E

K

R

R

A

A

S

L

Q

E

V

C

L

L

T

K

Q

M

V

A

G

E

L

L

E

E

D

E

R

R

M
x
F

-

A

D

N

H

H

K

K

P

P

N

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

A

N

N

H

N

P

P

S

P

I

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

T

S

G

V

E

E

K

I

I

M

M

K

Q

L

L

F

L

N

K

E

K

I

L

H

N

-

E

A

E

N

D

G

G

N

K

T

T

V

V

I

V

L

V

V

V

T

T

H
|
H

E

D

E

I

D

N

I

V

A

A

A

R

Y

F

A

G

H

E

R

R

V

I

I

I

R

Y

L

L

R

K

D

D

G

G

V

E

I

V

E

E

S

R

D

E

binding adenosine-5'-triphosphate: Y11 (≠ F15), S40 (= S44), G41 (= G45), S42 (= S46), G43 (= G47), K44 (= K48), S45 (= S49), T46 (= T50), F138 (≠ M139), Q145 (= Q145), S147 (= S147), G149 (= G149), Q150 (= Q150), H204 (= H203)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
45% identity, 93% coverage: 7:222/233 of query aligns to 8:216/223 of 2pclA

query
sites
2pclA

D

N

I

I

K

K

S

K

I

V

T

I

R

R

N

G

F

Y

P

-

L

-

G

-

N

-

E

-

V

-

V

-

K

E

V

I

L

L

K

K

G

G

I

I

D

S

L

L

T

S

I

V

N

K

K

K

G

G

E

E

Y

F

V

V

A

S

L

I

M

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

M

L

N

Y

L

I

L

L

G

G

C

L

L

L

D

D

T

A

P

P

T

T

G

E

G

G

T

K

Y

V

I

F

L

L

N

E

G

G

K

K

D

E

V

V

S

D

K

Y

M

T

S

N

D

E

N

K

E

E

L

L

A

S

E

L

I

L

R

R

N

N

K

R

E

K

I

L

G

G

F

F

V

V

F

F

Q

Q

T

F

F

H

N

Y

L

L

L

I

P

P

R

E

T

L

T

T

A

A

L

L

D

E

N

N

V

V

A

I

L

V

P

P

M

M

V

L

Y

K

A

M

G

G

Y

K

K

P

K

K

P

K

E

E

R

A

N

K

E

E

R

R

A

G

S

E

Q

Y

V

L

L

L

T

S

Q

E

V

L

G

G

L

L

E

G

D

D

R

K

M

L

D

S

H

R

K

K

P

P

N

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

A

N

N

H

E

P

P

S

I

I

L

I

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

S

S

K

A

T

N

S

T

V

K

E

R

I

V

M

M

K

D

L

I

F

F

N

L

E

K

I

I

H

N

A

E

N

G

G

G

N

T

T

S

V

I

I

V

L

M

V

V

T
|
T

H

H

E

E

E

R

D

E

I

L

A

A

A

E

Y

L

A

T

H

H

R

R

V

T

I

L

R

E

L

M

R

K

D

D

G

G

V

K

I

V

binding magnesium ion: E165 (= E171), P166 (= P172), T167 (= T173), T196 (= T202)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
43% identity, 97% coverage: 1:225/233 of query aligns to 1:226/233 of P75957

query
sites
P75957

M

M

A

N

Q

K

P

I

I

L

I

L

D

Q

I

C

K

D

S

N

I

L

T

C

R

K

N

R

F

Y

P

Q

L

E

G

G

N

S

E

V

V

Q

V

T

K

D

V

V

L

L

K

H

G

N

I

V

D

S

L

F

T

S

I

V

N

G

K

E

G

G

E

E

Y

M

V

M

A

A

L

I

M

V

G
|
G

P

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

M

L

N

H

L

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

I

L

F

N

N

G

G

K

Q

D

P

V

M

S

S

K

K

M

L

S

S

D

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

K

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

T

F

F

H

N

H

L

L

L

L

P

P

R

D

T

F

T

T

A

A

L

L

D

E

N

N

V

V

A

A

L

M

P

P

M

L

V

L

Y

I

A

G

G

K

Y

K

K

K

K

P

P

A

E

E

R

I

N

N

E

S

R

R

A

A

S

L

Q

E

V

M

L

L

T

K

Q

A

V

V

G

G

L

L

E

D

D

H

R

R

M

A

D

N

H

H

K

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

V

N

N

H

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

K

Q

L

L

F

L

N

G

E

E

I

L

H

N

-

R

A

L

N

Q

G

G

N

T

T

A

V

F

I

L

L

V

V

V

T

T

H

H

E

D

E

L

D

Q

I

L

A

A

A

K

Y

R

A

M

H

S

R

R

V

Q

I

L

R

E

L

M

R

R

D

D

G

G

V

R

I

L

E

T

S

A

D

E

G42 (= G42) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
44% identity, 95% coverage: 5:225/233 of query aligns to 2:223/226 of 7mdyC

query
sites
7mdyC

I

L

I

L

D

Q

I

C

K

D

S

N

I

L

T

C

R

K

N

R

F
x
Y

P

Q

L

E

G

G

N

S

E

V

V

Q

V

T

K

D

V

V

L

L

K

H

G

N

I

V

D

S

L

F

T

S

I

V

N

G

K

E

G

G

E

E

Y

M

V

M

A

A

L

I

M

V

G

G

P

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

N

H

L

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

I

L

F

N

N

G

G

K

Q

D

P

V

M

S

S

K

K

M

L

S

S

D

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

K

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

T

F

F

H

N

H

L

L

L

L

P

P

R

D

T

F

T

T

A

A

L

L

D

E

N

N

V

V

A

A

L

M

P

P

M

L

V

L

Y

I

A

G

G

K

Y

K

K

K

K

P

P

A

E

E

R

I

N

N

E

S

R

R

A

A

S

L

Q

E

V

M

L

L

T

K

Q

A

V

V

G

G

L

L

E

D

D

H

R

R

M

A

D

N

H
|
H

K

R

P

P

N

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

R

R

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

V

N

N

H

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

N
|
N

L

L

D

D

S

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

K

Q

L

L

F

L

N

G

E

E

I

L

H

N

-

R

A

L

N

Q

G

G

N

T

T

A

V

F

I

L

L

V

V

V

T

T

H
|
H

E

D

E

L

D

Q

I

L

A

A

A

K

Y

R

A

M

H

S

R

R

V

Q

I

L

R

E

L

M

R

R

D

D

G

G

V

R

I

L

E

T

S

A

D

E

binding adp orthovanadate: Y12 (≠ F15), G42 (= G45), S43 (= S46), G44 (= G47), K45 (= K48), S46 (= S49), T47 (= T50), Q91 (= Q94), H138 (= H141), E142 (≠ Q145), S144 (= S147), G145 (= G148), G146 (= G149), E168 (= E171), N172 (= N175), H201 (= H203)
binding magnesium ion: S46 (= S49), Q91 (= Q94)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
45% identity, 93% coverage: 5:220/233 of query aligns to 2:218/222 of 7arlD

query
sites
7arlD

I

L

I

L

D

Q

I

C

K

D

S

N

I

L

T

C

R

K

N

R

F

Y

P

Q

L

E

G

G

N

S

E

V

V

Q

V

T

K

D

V

V

L

L

K

H

G

N

I

V

D

S

L

F

T

S

I

V

N

G

K

E

G

G

E

E

Y

M

V

M

A

A

L

I

M

V

G

G

P

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

L

M

L

N

H

L

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

I

L

F

N

N

G

G

K

Q

D

P

V

M

S

S

K

K

M

L

S

S

D

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

K

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q

Q

T

F

F

H

N

H

L

L

L

L

P

P

R

D

T

F

T

T

A

A

L

L

D

E

N

N

V

V

A

A

L

M

P

P

M

L

V

L

Y

I

A

G

G

K

Y

K

K

K

K

P

P

A

E

E

R

I

N

N

E

S

R

R

A

A

S

L

Q

E

V

M

L

L

T

K

Q

A

V

V

G

G

L

L

E

D

D

H

R

R

M

A

D

N

H

H

K

R

P

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

R

R

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

V

N

N

H

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

K

Q

L

L

F

L

N

G

E

E

I

L

H

N

-

R

A

L

N

Q

G

G

N

T

T

A

V

F

I

L

L

V

V

V

T

T

H

H

E

D

E

L

D

Q

I

L

A

A

A

K

Y

R

A

M

H

S

R

R

V

Q

I

L

R

E

L

M

R

R

D

D

G

G

binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (= S49)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
43% identity, 95% coverage: 5:225/233 of query aligns to 4:225/229 of 7v8iD

query
sites
7v8iD

I

L

I

L

D

Q

I

C

K

D

S

N

I

L

T

C

R

K

N

R

F

Y

P

Q

L

E

G

G

N

S

E

V

V

Q

V

T

K

D

V
|
V

L

L

K

H

G

N

I

V

D

S

L

F

T

S

I

V

N

G

K

E

G

G

E

E

Y

M

V

M

A

A

L

I

M

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

N

H

L

L

L

L

G

G

C

G

L

L

D

D

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

I

L

F

N

N

G

G

K

Q

D

P

V

M

S

S

K

K

M

L

S

S

D

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

K

Q

E

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

T

F

F

H

N

H

L

L

L

L

P

P

R

D

T

F

T

T

A

A

L

L

D

E

N

N

V

V

A

A

L

M

P

P

M

L

V

L

Y

I

A

G

G

K

Y

K

K

K

K

P

P

A

E

E

R

I

N

N

E

S

R

R

A

A

S

L

Q

E

V

M

L

L

T

K

Q

A

V

V

G

G

L

L

E

D

D

H

R
|
R

M

A

D

N

H
|
H

K

R

P

P

N

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

R

R

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

V

N

N

H

N

P

P

S

R

I

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

S

A

V

D

E

S

I

I

M

F

K

Q

L

L

F

L

N

G

E

E

I

L

H

N

-

R

A

L

N

Q

G

G

N

T

T

A

V

F

I

L

L

V

V

V

T

T

H
|
H

E

D

E

L

D

Q

I

L

A

A

A

K

Y

R

A

M

H

S

R

R

V

Q

I

L

R

E

L

M

R

R

D

D

G

G

V

R

I

L

E

T

S

A

D

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (= S49), T49 (= T50), Q93 (= Q94), R137 (= R138), H140 (= H141), E144 (≠ Q145), S146 (= S147), G148 (= G149), E149 (≠ Q150), H203 (= H203)
binding magnesium ion: S48 (= S49), Q93 (= Q94)

8g4cB Bceabs atpgs high res tm (see paper)
42% identity, 93% coverage: 5:220/233 of query aligns to 3:219/248 of 8g4cB

query
sites
8g4cB

I

I

I

L

D

E

I

A

K

N

S

K

I

I

T

R

R

K

N

S

F
x
Y

P

-

L

-

G

G

N

N

E

K

V

L

V

N

K

K

-

Q

-

E

V

V

L

L

K

K

G

G

I

I

D

D

L

I

T

H

I

I

N

E

K

K

G

G

E

E

Y

F

V

V

A

S

L

I

M

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

L

L

M

L

N

N

L

V

L

L

G

S

C

S

L

I

D

D

T

Q

P

V

T

S

G

H

G

G

T

T

Y

I

I

H

L

I

N

N

G

G

K

N

D

D

V

M

S

T

K

A

M

M

S

K

D

E

N

K

E

Q

L

L

A

A

E

E

I

F

R

R

N

K

K

Q

E

H

I

L

G

G

F

F

V

I

F

F

Q

Q

T

D

F

Y

N

N

L

L

L

L

P

D

R

T

T

L

T

T

A

V

L

K

D

E

N

N

V

I

A

L

L

L

P

P

M

L

V

S

Y

I

A

T

G

K

Y

L

K

S

K

K

P

K

E

E

R

A

N

N

E

R

R

K

A

F

S

E

Q

E

V

V

L

A

T

K

Q

E

V

L

G

G

L

I

E

Y

D

E

R

L

M

R

D

D

H

K

K

Y

P

P

N

N

Q

E

L

I

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

T

A

S

V

A

A

G

R

R

A

A

L

F

V

I

N

H

H

D

P

P

S

S

I

I

I

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

S

S

A

V

S

E

D

I

L

M

L

K

N

L

K

F

L

N

S

E

Q

I

L

H

N

A

Q

N

K

G

R

N

N

-

A

T

T

V

I

I

I

L

M

V

V

T

T

H

H

E

D

E

P

D

V

I

A

A

A

A

S

Y

Y

A

C

H

G

R

R

V

V

I

I

R

F

L

I

R

K

D

D

G

G

binding phosphothiophosphoric acid-adenylate ester: Y13 (≠ F15), S42 (= S44), S44 (= S46), T47 (≠ S49), D168 (= D170)

7tchB Bceab e169q variant atp-bound conformation (see paper)
42% identity, 93% coverage: 5:220/233 of query aligns to 2:218/245 of 7tchB

query
sites
7tchB

I

I

I

L

D

E

I

A

K

N

S

K

I

I

T

R

R

K

N

S

F
x
Y

P

-

L

-

G

G

N

N

E

K

V

L

V

N

K

K

-
x
Q

-

E

V
|
V

L

L

K

K

G

G

I

I

D

D

L

I

T

H

I

I

N

E

K

K

G

G

E

E

Y

F

V

V

A

S

L

I

M

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

L

L

M

L

N

N

L

V

L

L

G

S

C

S

L

I

D

D

T

Q

P

V

T

S

G

H

G

G

T

T

Y

I

I

H

L

I

N

N

G

G

K

N

D

D

V

M

S

T

K

A

M

M

S

K

D

E

N

K

E

Q

L

L

A

A

E

E

I

F

R

R

N

K

K

Q

E

H

I

L

G

G

F

F

V

I

F

F

Q

Q

T

D

F

Y

N

N

L

L

L

L

P

D

R

T

T

L

T

T

A

V

L

K

D

E

N

N

V

I

A

L

L

L

P

P

M

L

V

S

Y

I

A

T

G

K

Y

L

K

S

K

K

P

K

E

E

R

A

N

N

E

R

R

K

A

F

S

E

Q

E

V

V

L

A

T

K

Q

E

V

L

G

G

L

I

E

Y

D

E

R

L

M

R

D

D

H

K

K

Y

P

P

N

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

Q

Q

R

K

Q

Q

R

R

V

T

A

S

V

A

A

G

R

R

A

A

L

F

V

I

N

H

H

D

P

P

S

S

I

I

I

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

S

S

A

V

S

E

D

I

L

M

L

K

N

L

K

F

L

N

S

E

Q

I

L

H

N

A

Q

N

K

G

R

N

N

-

A

T

T

V

I

I

I

L

M

V

V

T

T

H

H

E

D

E

P

D

V

I

A

A

A

A

S

Y

Y

A

C

H

G

R

R

V

V

I

I

R

F

L

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: Y12 (≠ F15), Q19 (vs. gap), V21 (= V24), S41 (= S44), G42 (= G45), S43 (= S46), K45 (= K48), T46 (≠ S49), E142 (≠ Q145), G145 (= G148), G146 (= G149)

8igqA Cryo-em structure of mycobacterium tuberculosis adp bound ftsex/ripc complex in peptidisc (see paper)
44% identity, 86% coverage: 25:225/233 of query aligns to 20:220/227 of 8igqA

query
sites
8igqA

L

L

K

D

G

D

I

I

D

N

L

V

T

K

I

I

N

D

K

K

G

G

E

E

Y

F

V

V

A

F

L

L

M

I

G

G

P

P

S

S

G
|
G

S
|
S

G

G

K
|
K

S
|
S

T

T

L

F

M

M

N

R

L

L

L

L

G

L

C

A

L

A

D

E

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

R

L

V

N

S

G

K

K

F

D

H

V

V

S

N

K

K

M

L

S

R

D

G

N

R

E

H

L

V

A

P

E

K

I

L

R

R

N

-

K

Q

E

V

I

I

G

G

F

C

V

V

F

F

Q

Q

T

D

F

F

N

R

L

L

L

L

P

Q

R

Q

T

K

T

T

A

V

L

Y

D

D

N

N

V

V

A

A

L

F

P

A

M

L

V

E

Y

V

A

I

G

G

Y

K

K

R

K

T

P

D

E

A

R

I

N

N

E

R

R

V

A

V

S

P

Q

E

V

V

L

L

T

E

Q

T

V

V

G

G

L

L

E

S

D

G

R

K

M

A

D

N

H

R

K

L

P

P

N

D

Q

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

F

V

V

N

N

H

R

P

P

S

L

I

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

P

K

E

T

T

S

S

V

R

E

D

I

I

M

M

K

D

L

L

F

L

N

E

E

R

I

I

H

N

A

R

N

T

G

G

N

T

T

T

V

V

I

L

L

M

V

A

T

T

H

H

E

D

E

H

D

H

I

I

A

V

-

D

A

S

Y

M

A

R

H

Q

R

R

V

V

I

V

R

E

L

L

R

S

D

L

G

G

V

R

I

L

E

V

S

R

D

D

binding adenosine-5'-diphosphate: G40 (= G45), S41 (= S46), K43 (= K48), S44 (= S49)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

8iddA Cryo-em structure of mycobacterium tuberculosis atp bound ftsex/ripc complex in peptidisc (see paper)
44% identity, 86% coverage: 25:225/233 of query aligns to 20:220/225 of 8iddA

query
sites
8iddA

L

L

K

D

G

D

I

I

D

N

L

V

T

K

I

I

N

D

K

K

G

G

E

E

Y

F

V

V

A

F

L

L

M

I

G

G

P

P

S
|
S

G

G

S

S

G
|
G

K
|
K

S
|
S

T

T

L

F

M

M

N

R

L

L

L

L

G

L

C

A

L

A

D

E

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

R

L

V

N

S

G

K

K

F

D

H

V

V

S

N

K

K

M

L

S

R

D

G

N

R

E

H

L

V

A

P

E

K

I

L

R

R

N

-

K

Q

E

V

I

I

G

G

F

C

V

V

F

F

Q

Q

T

D

F

F

N

R

L

L

L

L

P

Q

R

Q

T

K

T

T

A

V

L

Y

D

D

N

N

V

V

A

A

L

F

P

A

M

L

V

E

Y

V

A

I

G

G

Y

K

K

R

K

T

P

D

E

A

R

I

N

N

E

R

R

V

A

V

S

P

Q

E

V

V

L

L

T

E

Q

T

V

V

G

G

L

L

E

S

D

G

R

K

M

A

D

N

H

R

K

L

P

P

N

D

Q

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

F

V

V

N

N

H

R

P

P

S

L

I

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

P

K

E

T

T

S

S

V

R

E

D

I

I

M

M

K

D

L

L

F

L

N

E

E

R

I

I

H

N

A

R

N

T

G

G

N

T

T

T

V

V

I

L

L

M

V

A

T

T

H

H

E

D

E

H

D

H

I

I

A

V

-

D

A

S

Y

M

A

R

H

Q

R

R

V

V

I

V

R

E

L

L

R

S

D

L

G

G

V

R

I

L

E

V

S

R

D

D

binding adenosine-5'-triphosphate: S39 (= S44), G42 (= G47), K43 (= K48), S44 (= S49)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12

A5U7B7 Cell division ATP-binding protein FtsE from Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) (see 2 papers)
44% identity, 86% coverage: 25:225/233 of query aligns to 19:219/229 of A5U7B7

query
sites
A5U7B7

L

L

K

D

G

D

I

I

D

N

L

V

T

K

I

I

N

D

K

K

G

G

E

E

Y

F

V

V

A

F

L

L

M

I

G

G

P

P

S

S

G

G

S

S

G

G

K
|
K

S

S

T

T

L

F

M

M

N

R

L

L

L

L

G

L

C

A

L

A

D

E

T

T

P

P

T

T

G

S

G

G

T

D

Y

V

I

R

L

V

N

S

G

K

K

F

D

H

V

V

S

N

K

K

M

L

S

R

D

G

N

R

E

H

L

V

A

P

E

K

I

L

R

R

N

-

K

Q

E

V

I

I

G

G

F
x
C

V

V

F

F

Q

Q

T

D

F

F

N

R

L

L

L

L

P

Q

R

Q

T

K

T

T

A

V

L

Y

D

D

N

N

V

V

A

A

L

F

P

A

M

L

V

E

Y

V

A

I

G

G

Y

K

K

R

K

T

P

D

E

A

R

I

N

N

E

R

R

V

A

V

S

P

Q

E

V

V

L

L

T

E

Q

T

V

V

G

G

L

L

E

S

D

G

R

K

M

A

D

N

H

R

K

L

P

P

N

D

Q

E

L

L

S

S

G

G

G

G

Q

E

R

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

F

V

V

N

N

H

R

P

P

S

L

I

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

P

K

E

T

T

S

S

V

R

E

D

I

I

M

M

K

D

L

L

F

L

N

E

E

R

I

I

H

N

A

R

N

T

G

G

N

T

T

T

V

V

I

L

L

M

V

A

T

T

H

H

E

D

E

H

D

H

I

I

A

V

-

D

A

S

Y

M

A

R

H

Q

R

R

V

V

I

V

R

E

L

L

R

S

D

L

G

G

V

R

I

L

E

V

S

R

D

D

K42 (= K48) mutation to R: Does not bind ATP. Does not affect dimerization.
C84 (≠ F91) mutation to A: Does not affect ATPase activity.

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
41% identity, 94% coverage: 5:222/233 of query aligns to 1:214/240 of 4ymuJ

query
sites
4ymuJ

I

M

I

I

D

F

I

V

K

N

S

D

I

V

T

Y

R

K

N

N

F
|
F

P

-

L

-

G

G

N

S

E

-

V

-

V

L

K

E

V
|
V

L

L

K

K

G

G

I

V

D

T

L

L

T

K

I

V

N

N

K

K

G

G

E

E

Y

V

V

V

A

V

L

I

M

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

N

R

L

C

L

I

G

N

C

L

L

L

D

E

T

E

P

P

T

T

G

K

G

G

T

E

Y

V

I

F

L

I

N

D

G

G

K

V

D

K

V

I

S

N

K

N

M

G

S

K

D

V

N

N

E

-

L

I

A

N

E

K

I

V

R

R

N

Q

K

K

E

-

I

V

G

G

F

M

V

V

F

F

Q

Q

T

H

F

F

N

N

L

L

L

F

P

P

R

H

T

L

T

T

A

A

L

I

D

E

N

N

V

I

A

T

L

L

-

A

P

P

M

V

V

K

Y

V

A

K

G

K

Y

M

K

N

K

K

P

K

E

E

R

A

N

E

E

E

R

L

A

A

S

V

Q

D

V

L

L

L

T

A

Q

K

V

V

G

G

L

L

E

L

D

D

R

K

M

K

D

D

H

Q

K

Y

P

P

N

I

Q

K

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

L

A

A

V

I

A

A

R

R

A

A

L

L

V

A

N

M

H

Q

P

P

S

E

I

V

I

M

L

L

A

F

D

D

E
|
E

P

P

T

T

G

S

N

A

L

L

D

D

S

P

K

E

T

M

S

V

V

K

E

E

I

V

M

L

K

N

L

V

F

M

N

K

E

Q

I

L

H

A

A

N

N

E

G

G

N

M

T

T

V

M

I

V

L

V

V

V

T

T

H
|
H

E

E

E

M

D

G

I

F

A

A

-

R

A

E

Y

V

A

G

H

D

R

R

V

V

I

I

R

F

L

M

R

D

D

D

G

G

V

V

I

I

binding adenosine-5'-triphosphate: F11 (= F15), V16 (= V24), S36 (= S44), G37 (= G45), S38 (= S46), G39 (= G47), K40 (= K48), S41 (= S49), T42 (= T50), E162 (= E171), H194 (= H203)
binding magnesium ion: S41 (= S49), E162 (= E171)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 87% coverage: 5:207/233 of query aligns to 1:203/343 of P30750

query
sites
P30750

I

M

I

I

D

K

I

L

K

S

S

N

I

I

T

T

R

K

N

V

F

F

P

H

L

Q

G

G

N

T

E

R

V

T

V

I

K

Q

V

A

L

L

K

N

G

N

I

V

D

S

L

L

T

H

I

V

N

P

K

A

G

G

E

Q

Y

I

V

Y

A

G

L

V

M

I

G

G

P

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

I

N

R

L

C

L

V

G

N

C

L

L

L

D

E

T

R

P

P

T

T

G

E

G

G

T

S

Y

V

I

L

L

V

N

D

G

G

K

Q

D

E

V

L

S

T

K

T

M

L

S

S

D

E

N

S

E

E

L

L

A

T

E

K

I

A

R

R

N

-

K

R

E

Q

I

I

G

G

F

M

V

I

F

F

Q

Q

T

H

F

F

N

N

L

L

L

L

P

S

R

S

T

R

T

T

A

V

L

F

D

G

N

N

V

V

A

A

L

L

P

P

M

L

V

E

Y

L

A

D

G

N

Y

T

K

P

K

K

P

D

E

E

R

V

N

K

E

R

R

R

A

V

S

T

Q

E

V

L

L

L

T

S

Q

L

V

V

G

G

L

L

E

G

D

D

R

K

M

H

D

D

H

S

K

Y

P

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

R

K

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

V

A

N

S

H

N

P

P

S

K

I

V

I

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

S

P

K

A

T

T

S

T

V

R

E

S

I

I

M

L

K

E

L

L

F

L

N

K

E

D

I

I

H

N

A

R

N

R

-

L

G

G

N

L

T

T

V

I

I

L

L

L

V

I

T

T

H

H

E

E

E

M

D

D

I

V

40:46 (vs. 44:50, 86% identical) binding ATP
E166 (= E171) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
41% identity, 85% coverage: 23:220/233 of query aligns to 16:213/219 of 8w6iD

query
sites
8w6iD

K

Q

V

A

L

L

K

Q

G

G

I

V

D

T

L

F

T

H

I

M

N

Q

K

P

G

G

E

E

Y

M

V

A

A

F

L

L

M

T

G

G

P

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

N

K

L

L

L

I

G

C

C

G

L

I

D

E

T

R

P

P

T

S

G

A

G

G

T

K

Y

I

I

W

L

F

N

S

G

G

K

H

D

D

V

I

S

T

K

R

M

L

S

K

D

N

N

R

E

E

L

V

A

P

E

F

I

L

R

R

N

-

K

R

E

Q

I

I

G

G

F

M

V

I

F

F

Q
|
Q

T

D

F

H

N

H

L

L

L

L

P

M

R

D

T

R

T

T

A

V

L

Y

D

D

N

N

V

V

A

A

L

I

P

P

M

L

V

I

Y

I

A

A

G

G

Y

A

K

S

K

G

P

D

E

D

R

I

N

R

E

R

R

R

A

V

S

S

Q

A

V

A

L

L

T

D

Q

K

V

V

G

G

L

L

E

L

D

D

R
x
K

M

A

D

K

H

N

K

F

P

P

N

I

Q
|
Q

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

R

Q

Q

Q

R

R

V

V

A

G

V

I

A

A

R

R

A

A

L

V

V

V

N

N

H

K

P

P

S

A

I

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

D

K

A

T

L

S

S

V

E

E

G

I

I

M

L

K

R

L

L

F

F

N

E

E

E

I

F

H

N

A

R

N

V

G

G

N

V

T

T

V

V

I

L

L

M

V

A

T

T

H
|
H

E

D

E

I

D

N

-

L

I

I

A

S

A

R

Y

R

A

S

H

Y

R

R

V

M

I

L

R

T

L

L

R

S

D

D

G

G

binding phosphothiophosphoric acid-adenylate ester: S37 (= S44), G38 (= G45), A39 (≠ S46), G40 (= G47), K41 (= K48), S42 (= S49), T43 (= T50), Q86 (= Q94), K130 (≠ R138), Q137 (= Q145), S139 (= S147), G140 (= G148), G141 (= G149), E142 (≠ Q150), H195 (= H203)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 11

Query Sequence

>WP_035132405.1 NCBI__GCF_000769915.1:WP_035132405.1
MAQPIIDIKSITRNFPLGNEVVKVLKGIDLTINKGEYVALMGPSGSGKSTLMNLLGCLDT
PTGGTYILNGKDVSKMSDNELAEIRNKEIGFVFQTFNLLPRTTALDNVALPMVYAGYKKP
ERNERASQVLTQVGLEDRMDHKPNQLSGGQRQRVAVARALVNHPSIILADEPTGNLDSKT
SVEIMKLFNEIHANGNTVILVTHEEDIAAYAHRVIRLRDGVIESDNINPNPHR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory