SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035133104.1 NCBI__GCF_000769915.1:WP_035133104.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6dbbA Crystal structure of a putative aldehyde dehydrogenase family protein burkholderia cenocepacia j2315 in complex with partially reduced nadh
56% identity, 93% coverage: 35:515/515 of query aligns to 19:504/504 of 6dbbA

query
sites
6dbbA

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active site: N152 (= N168), E259 (= E268), C293 (= C302), E471 (= E482)
binding nicotinamide-adenine-dinucleotide: I148 (= I164), S149 (= S165), A150 (= A166), F151 (= F167), N152 (= N168), K175 (= K191), S177 (= S193), R218 (= R227), T236 (= T245), G237 (= G246), S238 (= S247), M241 (≠ V250), E259 (= E268), L260 (= L269), G261 (= G270), C293 (= C302), E391 (= E402), F393 (= F404)
binding beta-6-hydroxy-1,4,5,6-tetrhydronicotinamide adenine dinucleotide: I148 (= I164), S149 (= S165), A150 (= A166), F151 (= F167), N152 (= N168), K175 (= K191), S177 (= S193), R218 (= R227), T236 (= T245), G237 (= G246), S238 (= S247), M241 (≠ V250), E259 (= E268), L260 (= L269), G261 (= G270), C293 (= C302), E391 (= E402), F393 (= F404)

2jg7A Crystal structure of seabream antiquitin and elucidation of its substrate specificity (see paper)
56% identity, 97% coverage: 14:513/515 of query aligns to 13:509/509 of 2jg7A

query
sites
2jg7A

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active site: N166 (= N168), K189 (= K191), E267 (= E268), C301 (= C302), E398 (= E402), E478 (= E482)
binding nicotinamide-adenine-dinucleotide: I162 (= I164), T163 (≠ S165), A164 (= A166), F165 (= F167), N166 (= N168), K189 (= K191), P192 (≠ E194), A226 (≠ R227), G229 (= G230), T230 (≠ D231), F243 (= F244), T244 (= T245), G245 (= G246), S246 (= S247), V249 (= V250), E267 (= E268), L268 (= L269), C301 (= C302), E398 (= E402), F400 (= F404)

P49419 Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 from Homo sapiens (Human) (see 5 papers)
55% identity, 97% coverage: 14:513/515 of query aligns to 42:538/539 of P49419

query
sites
P49419

L

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P
x
S

L
|
L

C
x
I

G
x
S

I
x
V

A
|
A

C
x
V

Q
x
T

N
x
K

I
|
I

K
x
A

T
x
K

V
|
V

L
|
L

E
|
E

R
x
D

N
|
N

N
x
K

I
x
L

P
|
P

E
x
G

G
x
A

V
x
I

S
x
C

C
x
S

L
|
L

I
x
T

-
x
C

N
x
G

G
|
G

R
x
A

D
|
D

N
x
I

G
|
G

D
x
T

R
x
A

M
|
M

N
x
A

T
x
K

D
|
D

K
x
E

R
|
R

L
x
V

P
x
N

L
|
L

V
x
L

S
|
S

F
|
F

T
|
T

G
|
G

S
|
S

T
|
T

R
x
Q

V
|
V

G
|
G

R
x
K

H
x
Q

V
|
V

S
x
G

K
x
L

T
x
M

V
|
V

A
x
Q

E
|
E

R
|
R

F
|
F

G
|
G

N
x
R

T
x
S

I
x
L

L
|
L

E
|
E

L
|
L

G
|
G

N
|
N

A
|
A

I
|
I

V
x
A

S
x
F

E
|
E

H
x
D

A
|
A

D
|
D

I
x
L

N
x
S

M
x
L

V
|
V

L
x
V

V
x
P

G
x
S

A
|
A

V
x
L

F
|
F

G
x
A

A
|
A

V
|
V

G
|
G

T
|
T

A
|
A

G
|
G

Q
|
Q

R
|
R

C
|
C

T
|
T

T
x
A

R
|
R

L
|
L

I
x
F

V
x
I

H
|
H

E
|
E

S
|
S

V
x
I

Y
x
H

D
|
D

K
x
E

T
x
V

I
x
V

E
x
N

V
x
R

L
|
L

K
|
K

S
x
K

A
|
A

Y
|
Y

G
x
A

Q
|
Q

L
x
I

K
x
R

I
x
V

G
|
G

N
|
N

P
|
P

L
x
W

D
|
D

S
x
P

N
|
N

N
x
V

H
x
L

V
x
Y

G
|
G

P
|
P

L
|
L

I
x
H

D
x
T

K
|
K

D
x
Q

S
x
A

V
|
V

Q
x
S

N
x
M

Y
x
F

L
|
L

D
x
G

A
|
A

I
x
V

E
|
E

K
x
E

A
|
A

K
|
K

Q
x
K

E
|
E

G
|
G

N
x
T

V
|
V

I
x
V

V
x
Y

E
x
G

G
|
G

G
x
K

V
|
V

M
|
M

Q
x
D

G

-

E

-

G

-

Y

-

E
x
R

S
x
P

G
|
G

C
x
N

Y
|
Y

V
|
V

K
x
E

P
|
P

C
x
T

I
|
I

I
x
V

E
x
T

A
x
G

K
x
L

N
x
G

S
x
H

F
x
D

E
x
A

I
x
S

V
x
I

Q
x
A

H
|
H

-
x
T

E
|
E

T
|
T

F
|
F

A
|
A

P
|
P

I
|
I

L
|
L

Y
|
Y

V
|
V

M
x
F

K
|
K

Y
x
F

N
x
K

D
x
N

L
x
E

E
|
E

A
x
V

I
x
F

A
|
A

M
x
W

Q
x
N

N
|
N

D
x
E

V
|
V

P
x
K

Q
|
Q

G
|
G

L
|
L

S
|
S

I
|
I

F
|
F

T
|
T

S
x
K

N
x
D

M
x
L

R
x
G

E
x
R

M
x
I

E
x
F

L
x
R

F
x
W

L
|
L

S
x
G

H
x
P

A
x
K

G
|
G

S
|
S

D
|
D

C
|
C

G
|
G

I
|
I

A
x
V

N
|
N

V
|
V

N
|
N

I
|
I

G
x
P

T
|
T

S
|
S

G
|
G

A
|
A

E
|
E

I
|
I

G
|
G

A
|
A

F
|
F

G
|
G

E
|
E

K
|
K

E
x
H

T
|
T

G
|
G

R
|
R

E
|
E

S
|
S

G
|
G

S
|
S

D
|
D

A
|
A

W
|
W

K
|
K

A
x
Q

Y
|
Y

M
|
M

R
|
R

Q
x
S

T
|
T

N
x
C

T
|
T

I
|
I

N
|
N

Y
|
Y

G
x
S

S
x
K

E
x
D

L
|
L

P
|
P

L
|
L

A
|
A

Q
|
Q

G
|
G

I
|
I

R
x
K

F
|
F

TAF 192:194 (≠ SAF 165:167) binding NAD(+)
A199 (= A172) to V: in EPEO4; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912709
K218 (= K191) binding NAD(+)
GT 258:259 (≠ GD 230:231) binding NAD(+)
GS 274:275 (= GS 246:247) binding NAD(+)
EL 296:297 (= EL 268:269) binding NAD(+)
C330 (= C302) active site, Nucleophile
E427 (= E402) binding NAD(+); to Q: in EPEO4; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912707
K439 (≠ N414) to Q: in dbSNP:rs12514417

Sites not aligning to the query:

110:539 natural variant: Missing (in EPEO4; loss of alpha-AASA dehydrogenase activity)

4zulA Structure aldh7a1 complexed with alpha-aminoadipate (see paper)
55% identity, 97% coverage: 14:513/515 of query aligns to 12:508/509 of 4zulA

query
sites
4zulA

L

L

S

K

K

E

L

L

G

G

I

L

Q

R

E

E

I

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

S

S

N

W

S

G

F

-

A

G

N

R

G

G

E

E

I

V

I

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

T

E

L

P

I

I

A

A

K

R

V

V

K

R

A

Q

S

A

T

S

Q

V

E

A

D

D

Y

Y

E

E

A

E

A

T

V

V

A

K

K

K

A

A

Q

R

E

E

A

A

F

W

K

K

S

I

W

W

R

A

L

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

E

Y

K

K

I

E

Q

P

V

L

L

G

G

Q

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

L

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

M

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

P

P

V

V

G

G

I

L

V

V

G

G

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

Y

S

G

W

W

N

N

A

N

M

A

L

I

A

A

W

M

V

I

C

C

G

G

D

N

V

V

C

C

I

L

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

I

V

A

A

C

V

Q

T

N

K

I

I

K

A

T

K

V

V

L

L

E

E

R

D

N

N

N

K

I

L

P

P

E

G

G

A

V

I

S

C

C

S

L

L

I

T

-

C

N

G

G

G

R

A

D

D

N

I

G

G

D

T

R

A

M

M

N

A

T

K

D

D

K

E

R

R

L

V

P

N

L

L

V

L

S

S

F

F

T

T

G

G

S

S

T

T

R

Q

V

V

G

G

R

K

H

Q

V

V

S

G

K

L

T

M

V

V

A

Q

E

E

R

R

F

F

G

G

N

R

T

S

I

L

L

L

E
|
E

L

L

G

G

N

N

A

A

I

I

V

A

S

F

E

E

H

D

A

A

D

D

I

L

N

S

M

L

V

V

L

V

V

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

T

T

T

A

R

R

L

L

I

F

V

I

H

H

E

E

S

S

V

I

Y

H

D

D

K

E

T

V

I

V

E

N

V

R

L

L

K

K

S

K

A

A

Y

Y

G

A

Q

Q

L

I

K

R

I

V

G

G

N

N

P

P

L

W

D

D

S

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

D

Q

S

A

V

V

Q

S

N

M

Y

F

L

L

D

G

A

A

I

V

E

E

K

E

A

A

K

K

Q

K

E

E

G

G

N

T

V

V

I

V

V

Y

E

G

G

G

G

K

V

V

M

M

Q

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

I

V

E

T

A

G

K

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

F

K

K

Y

F

N

K

D

N

L

E

E

E

A

V

I

F

A

A

M

W

Q

N

N

N

D

E

V

V

P

K

Q

Q

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

D

M

L

R

G

E

R

M

I

E

F

L

R

F

W

L

L

S

G

H

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G
|
G

A
|
A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

G

S

S

K

E

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

R

K

F

F

active site: N165 (= N168), K188 (= K191), E266 (= E268), C300 (= C302), E397 (= E402), E477 (= E482)
binding 2-aminohexanedioic acid: E119 (= E122), F166 (= F169), R299 (= R301), C300 (= C302), T301 (= T303), G459 (= G464), A460 (= A465), F466 (= F471)

4x0tA Structure aldh7a1 inactivated by 4-diethylaminobenzaldehyde and complexed with NAD+ (see paper)
55% identity, 97% coverage: 14:513/515 of query aligns to 12:508/509 of 4x0tA

query
sites
4x0tA

L

L

S

K

K

E

L

L

G

G

I

L

Q

R

E

E

I

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

S

S

N

W

S

G

F

-

A

G

N

R

G

G

E

E

I

V

I

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

T

E

L

P

I

I

A

A

K

R

V

V

K

R

A

Q

S

A

T

S

Q

V

E

A

D

D

Y

Y

E

E

A

E

A

T

V

V

A

K

K

K

A

A

Q

R

E

E

A

A

F

W

K

K

S

I

W

W

R

A

L

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

E

Y

K

K

I

E

Q

P

V

L

L

G

G

Q

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

L

V

G

G

E

E

V

V

Q

Q

E

E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

M

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

P

P

V

V

G

G

I

L

V

V

G

G

I

I

S
x
T

A
|
A

F
|
F

N
|
N

F
|
F

P

P

V

V

A

A

V
|
V

W

Y

S

G

W
|
W

N

N

A

N

M

A

L

I

A

A

W

M

V

I

C

C

G

G

D

N

V

V

C

C

I

L

W

W

K
|
K

P
x
G

S
x
A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

I

V

A

A

C

V

Q

T

N

K

I

I

K

A

T

K

V

V

L

L

E

E

R

D

N

N

N

K

I

L

P

P

E

G

G

A

V

I

S

C

C

S

L

L

I

T

-

C

N

G

G

G

R
x
A

D

D

N

I

G
|
G

D
x
T

R

A

M

M

N

A

T

K

D

D

K

E

R

R

L

V

P

N

L

L

V

L

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

V
|
V

G

G

R

K

H

Q

V

V

S

G

K

L

T

M

V

V

A

Q

E

E

R

R

F

F

G

G

N

R

T

S

I

L

L

L

E
|
E

L
|
L

G

G

N

N

A

A

I

I

V

A

S

F

E

E

H

D

A

A

D

D

I

L

N

S

M

L

V

V

L

V

V

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

T

A

R

R

L

L

I

F

V

I

H

H

E

E

S

S

V

I

Y

H

D

D

K

E

T

V

I

V

E

N

V

R

L

L

K

K

S

K

A

A

Y

Y

G

A

Q

Q

L

I

K

R

I

V

G

G

N

N

P

P

L

W

D

D

S

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

D

Q

S

A

V

V

Q

S

N

M

Y

F

L

L

D

G

A

A

I

V

E

E

K

E

A

A

K

K

Q

K

E

E

G

G

N

T

V

V

I

V

V

Y

E

G

G

G

G

K

V

V

M

M

Q

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

I

V

E

T

A

G

K

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

F

K

K

Y

F

N

K

D

N

L

E

E

E

A

V

I

F

A

A

M

W

Q

N

N

N

D

E

V

V

P

K

Q

Q

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

D

M

L

R

G

E

R

M

I

E

F

L

R

F

W

L

L

S

G

H

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

G

S

S

K

E

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

R

K

F

F

active site: N165 (= N168), K188 (= K191), E266 (= E268), C300 (= C302), E397 (= E402), E477 (= E482)
binding 4-(diethylamino)benzaldehyde: F166 (= F169), V170 (= V173), W173 (= W176), C300 (= C302), F466 (= F471)
binding nicotinamide-adenine-dinucleotide: T162 (≠ S165), A163 (= A166), F164 (= F167), N165 (= N168), K188 (= K191), G189 (≠ P192), A190 (≠ S193), A225 (≠ R227), G228 (= G230), T229 (≠ D231), F242 (= F244), T243 (= T245), G244 (= G246), S245 (= S247), V248 (= V250), E266 (= E268), L267 (= L269), C300 (= C302), E397 (= E402), F399 (= F404)

6o4dB Structure of aldh7a1 mutant w175a complexed with l-pipecolic acid (see paper)
55% identity, 97% coverage: 14:513/515 of query aligns to 13:509/510 of 6o4dB

query
sites
6o4dB

L

L

S

K

K

E

L

L

G

G

I

L

Q

R

E

E

I

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

S

S

N

W

S

G

F

-

A

G

N

R

G

G

E

E

I

V

I

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

T

E

L

P

I

I

A

A

K

R

V

V

K

R

A

Q

S

A

T

S

Q

V

E

A

D

D

Y

Y

E

E

A

E

A

T

V

V

A

K

K

K

A

A

Q

R

E

E

A

A

F

W

K

K

S

I

W

W

R

A

L

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

E

Y

K

K

I

E

Q

P

V

L

L

G

G

Q

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

L

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

M

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

P

P

V

V

G

G

I

L

V

V

G

G

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

Y

S

G

W

A

N

N

A

N

M

A

L

I

A

A

W

M

V

I

C

C

G

G

D

N

V

V

C

C

I

L

W

W

K

K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

I

V

A

A

C

V

Q

T

N

K

I

I

K

A

T

K

V

V

L

L

E

E

R

D

N

N

N

K

I

L

P

P

E

G

G

A

V

I

S

C

C

S

L

L

I

T

-

C

N

G

G

G

R

A

D

D

N

I

G

G

D

T

R

A

M

M

N

A

T

K

D

D

K

E

R

R

L

V

P

N

L

L

V

L

S

S

F

F

T

T

G

G

S

S

T

T

R

Q

V

V

G

G

R

K

H

Q

V

V

S

G

K

L

T

M

V

V

A

Q

E

E

R

R

F

F

G

G

N

R

T

S

I

L

L

L

E
|
E

L

L

G

G

N

N

A

A

I

I

V

A

S

F

E

E

H

D

A

A

D

D

I

L

N

S

M

L

V

V

L

V

V

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T
|
T

T

T

T

A

R

R

L

L

I

F

V

I

H

H

E

E

S

S

V

I

Y

H

D

D

K

E

T

V

I

V

E

N

V

R

L

L

K

K

S

K

A

A

Y

Y

G

A

Q

Q

L

I

K

R

I

V

G

G

N

N

P

P

L

W

D

D

S

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

D

Q

S

A

V

V

Q

S

N

M

Y

F

L

L

D

G

A

A

I

V

E

E

K

E

A

A

K

K

Q

K

E

E

G

G

N

T

V

V

I

V

V

Y

E

G

G

G

G

K

V

V

M

M

Q

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

I

V

E

T

A

G

K

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E

E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

F

K

K

Y

F

N

K

D

N

L

E

E

E

A

V

I

F

A

A

M

W

Q

N

N

N

D

E

V

V

P

K

Q

Q

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

D

M

L

R

G

E

R

M

I

E

F

L

R

F

W

L

L

S

G

H

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G
|
G

A
|
A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

G

S

S

K

E

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

R

K

F

F

active site: N166 (= N168), E267 (= E268), C301 (= C302), E478 (= E482)
binding pyridine-2-carboxylic acid: E120 (= E122), F167 (= F169), T302 (= T303), G460 (= G464), A461 (= A465), F467 (= F471)

2j6lA Structure of aminoadipate-semialdehyde dehydrogenase (see paper)
55% identity, 95% coverage: 14:501/515 of query aligns to 12:496/497 of 2j6lA

query
sites
2j6lA

L

L

S

K

K

E

L

L

G

G

I

L

Q

R

E

E

I

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

S

S

N

W

S

G

F

-

A

G

N

R

G

G

E

E

I

V

I

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

T

E

L

P

I

I

A

A

K

R

V

V

K

R

A

Q

S

A

T

S

Q

V

E

A

D

D

Y

Y

E

E

A

E

A

T

V

V

A

K

K

K

A

A

Q

R

E

E

A

A

F

W

K

K

S

I

W

W

R

A

L

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

E

Y

K

K

I

E

Q

P

V

L

L

G

G

Q

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

L

V

G

G

E

E

V

V

Q

Q

E

E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

M

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

P

P

V

V

G

G

I

L

V

V

G

G

I

I

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F

F

P

P

V

V

A

A

V

V

W

Y

S

G

W

W

N

N

A

N

M

A

L

I

A

A

W

M

V

I

C

C

G

G

D

N

V

V

C

C

I

L

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

I

V

A

A

C

V

Q

T

N

K

I

I

K

A

T

K

V

V

L

L

E

E

R

D

N

N

N

K

I

L

P

P

E

G

G

A

V

I

S

C

C

S

L

L

I

T

-

C

N

G

G

G

R
x
A

D

D

N

I

G
|
G

D
x
T

R

A

M

M

N

A

T

K

D

D

K

E

R

R

L

V

P

N

L

L

V

L

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

V
|
V

G

G

R

K

H

Q

V

V

S

G

K

L

T

M

V

V

A

Q

E

E

R

R

F

F

G

G

N

R

T

S

I

L

L

L

E
|
E

L
|
L

G

G

N

N

A

A

I

I

V

A

S

F

E

E

H

D

A

A

D

D

I

L

N

S

M

L

V

V

L

V

V

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

T

A

R

R

L

L

I

F

V

I

H

H

E

E

S

S

V

I

Y

H

D

D

K

E

T

V

I

V

E

N

V

R

L

L

K

K

S

K

A

A

Y

Y

G

A

Q

Q

L

I

K

R

I

V

G

G

N

N

P

P

L

W

D

D

S

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

D

Q

S

A

V

V

Q

S

N

M

Y

F

L

L

D

G

A

A

I

V

E

E

K

E

A

A

K

K

Q

K

E

E

G

G

N

T

V

V

I

V

V

Y

E

G

G

G

G

K

V

V

M

M

Q

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

I

V

E

T

A

G

K

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

F

K

K

Y

F

N

Q

D

N

L

E

E

E

A

V

I

F

A

A

M

W

Q

N

N

N

D

E

V

V

P

K

Q

Q

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

D

M

L

R

G

E

R

M

I

E

F

L

R

F

W

L

L

S

G

H

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F

F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

active site: N165 (= N168), K188 (= K191), E266 (= E268), C300 (= C302), E397 (= E402), E477 (= E482)
binding 1,4-dihydronicotinamide adenine dinucleotide: I161 (= I164), T162 (≠ S165), A163 (= A166), F164 (= F167), N165 (= N168), K188 (= K191), A225 (≠ R227), G228 (= G230), T229 (≠ D231), F242 (= F244), T243 (= T245), G244 (= G246), S245 (= S247), V248 (= V250), E266 (= E268), L267 (= L269), C300 (= C302), E397 (= E402), F399 (= F404)

4pxnA Structure of zm aldh7 in complex with NAD (see paper)
51% identity, 97% coverage: 11:508/515 of query aligns to 4:498/498 of 4pxnA

query
sites
4pxnA

Q

H

E

Q

A

F

L

L

S

A

K

E

L

L

G

G

I

L

Q

A

E

Q

I

R

N

N

E

P

G

G

T

A

S

F

T

A

G

C

S

G

N

A

S

W

F

G

A

G

N

S

G

G

E

P

I

T

I

V

E

T

S

S

Y

T

S

S

P

P

V

T

D

N

G

N

T

Q

L

V

I

I

A

A

K

E

V

V

K

V

A

E

S

A

T

S

Q

V

E

H

D

D

Y

Y

E

E

A

E

A

G

V

M

A

R

K

A

A

C

Q

F

E

D

A

A

F

A

K

K

S

T

W

W

R

M

L

A

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

A

Y

K

K

L

E

H

P

H

L

L

G

G

Q

R

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

P

E

E

G

G

L

I

G

G

E

E

V

V

Q

Q

E

E

M

I

I

I

D

D

I

M

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

Y

N

G

G

L

S

T

I

M

I

H

P

S

S

E

E

R

R

P

P

M

N

H

H

R

M

M

M

Y

M

E

E

Q

V

Y

W

H

N

P

P

V

L

G

G

I

V

V

V

G

G

I

V

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F

F

P

P

V

C

A

A

V

V

W

L

S

G

W

W

N

N

A

A

M

C

L

I

A

A

W

L

V

V

C

C

G

G

D

N

V

C

C

V

I

V

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

P

L

L

C

I

G

T

I

I

A

A

C

M

Q

T

N

K

I

I

I

V

K

A

T

S

V

V

L

L

E

E

R

K

N

N

I

L

P

P

E

G

G

A

V

I

-

F

S

T

C

S

L

F

I

C

N

G

G

G

R
x
T

D

E

N

I

G
|
G

D
x
Q

R

A

M

I

N

A

T

L

D

D

K

I

R

R

L

I

P

P

L

L

V

V

S

S

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

R

V
x
A

G

G

R

L

H

M

V
|
V

S

Q

K

Q

T

Q

V

V

A

S

E

A

R

R

F

F

G

G

N

K

T

C

I

L

L

L

E
|
E

L
|
L

G
x
S

G

G

N

N

A

A

I

I

V

V

S

M

E

D

H

D

A

A

D

D

I

I

N

Q

M

L

V

A

L

V

V

R

G

S

A

V

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

T

C

R

R

L

L

I

I

V

L

H

H

E

E

S

N

V

I

Y

Y

D

Q

K

T

T

F

I

L

E

D

V

Q

L

L

K

V

S

E

A

V

Y

Y

G

K

Q

Q

L

V

K

R

I

I

G

G

N

D

P

P

L

L

D

E

S

K

N

G

N

T

H

L

V

L

G

G

P

P

L

L

I

H

D

T

K

P

D

A

S

S

V

K

Q

E

N

N

Y

F

L

L

D

K

A

G

I

I

E

Q

K

T

A

I

K

K

Q

S

E

Q

G

G

N

K

V

I

I

L

V

F

E

G

G

G

G

S

V

A

M

I

Q

E

G

S

E

E

G

G

Y

N

E

-

S

-

G

-

C

-

Y

F

V

V

K

Q

P

P

C

T

I

I

I

V

E

E

A

I

K

T

N

P

S

S

F

A

E

P

I

V

V

V

Q

K

H

E

E
|
E

T

L

F
|
F

A

G

P

P

I

V

L

L

Y

Y

V

V

M

M

K

K

Y

F

N

Q

D

T

L

L

E

K

E

E

A

A

I

I

A

E

M

I

Q

N

N

N

D

S

V

V

P

P

Q

Q

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

R

M

P

R

D

E

I

M

I

E

F

L

K

F

W

L

L

S

G

H

P

A

H

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

N

G

G

A

A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

A

T

T

G

G

R

R

E
|
E

S

A

G

G

S

S

D

D

A

S

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

A

T

T

N

C

T

T

I

I

N

N

Y

Y

G

G

S

S

E

E

L

L

P

P

L

L

A

A

active site: N161 (= N168), K184 (= K191), E262 (= E268), C296 (= C302), E392 (= E402), E472 (= E482)
binding nicotinamide-adenine-dinucleotide: I157 (= I164), T158 (≠ S165), A159 (= A166), F160 (= F167), N161 (= N168), K184 (= K191), T221 (≠ R227), G224 (= G230), Q225 (≠ D231), F238 (= F244), T239 (= T245), G240 (= G246), S241 (= S247), A244 (≠ V250), V248 (= V254), E262 (= E268), L263 (= L269), S264 (≠ G270), C296 (= C302), E392 (= E402), F394 (= F404), F461 (= F471)

4x0uD Structure aldh7a1 inactivated by 4-diethylaminobenzaldehyde (see paper)
53% identity, 95% coverage: 14:501/515 of query aligns to 12:486/487 of 4x0uD

query
sites
4x0uD

L

L

S

K

K

E

L

L

G

G

I

L

Q

R

E

E

I

E

N

N

E

E

G

G

T

V

S

Y

T

N

G

G

S

S

N

W

S

G

F

-

A

G

N

R

G

G

E

E

I

V

I

I

E

T

S

T

Y

Y

S

C

P

P

V

A

D

N

G

N

T

E

L

P

I

I

A

A

K

R

V

V

K

R

A

Q

S

A

T

S

Q

V

E

A

D

D

Y

Y

E

E

A

E

A

T

V

V

A

K

K

K

A

A

Q

R

E

E

A

A

F

W

K

K

S

I

W

W

R

A

L

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

M

I

G

G

E

D

E

A

L

L

R

R

K

E

Y

K

K

I

E

Q

P

V

L

L

G

G

Q

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

L

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D
|
D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

M

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

Y

W

H

N

P

P

V

V

G

G

I

L

V

V

G

G

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A
|
A

V
|
V

W

Y

S

G

W

W

N

N

A

N

M

A

L

I

A

A

W

M

V

I

C

C

G

G

D

N

V

V

C

C

I

L

W

W

K
|
K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

C

I

G

S

I

V

A

A

C

V

Q

T

N

K

I

I

K

A

T

K

V

V

L

L

E

E

R

D

N

N

N

K

I

L

P

P

E

G

G

A

V

I

S

C

C

S

L

L

I

T

-

C

N

G

G

G

R

A

D

D

N

I

G

G

D

T

R

A

M

M

N

A

T

K

D

D

K

E

R

R

L

V

P

N

L

L

V

L

S

S

F

F

T

T

G

G

S

S

T

T

R

Q

V

V

G

G

R

K

H

Q

V

V

S

G

K

L

T

M

V

V

A

Q

E

E

R

R

F

F

G

G

N

R

T

S

I

L

L

L

E
|
E

L

L

G

G

N

N

A

A

I

I

V

A

S

F

E

E

H

D

A

A

D

D

I

L

N

S

M

L

V

V

L

V

V

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

T

A

R

R

L

L

I

F

V

I

H

H

E

E

S

S

V

I

Y

H

D

D

K

E

T

V

I

V

E

N

V

R

L

L

K

K

S

K

A

A

Y

Y

G

A

Q

Q

L

I

K

R

I

V

G

G

N

N

P

P

L

W

D

D

S

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

K

K

D

Q

S

A

V

V

Q

S

N

M

Y

F

L

L

D

G

A

A

I

V

E

E

K

E

A

A

K

K

Q

K

E

E

G

G

N

T

V

V

I

V

V

Y

E

G

G

G

G

K

V

V

M

M

Q

D

G

-

E

-

G

-

Y

-

E

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

I

V

E

T

A

G

K

L

N

G

S

H

F

D

E

A

I

S

V

I

Q

A

H

H

-

T

E
|
E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

F

K

K

Y

F

N

K

D

N

L

E

E

E

A

V

I

F

A

A

M

W

Q

N

N

N

D

E

V

V

P

-

Q

-

G

G

L

L

S

S

I

I

F

F

T

T

S

K

N

D

M

L

R

G

E

R

M

I

E

F

L

R

F

W

L

L

S

G

H

P

A

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

-

G

-

A

-

E

-

I

-

G

-

A

-

F
|
F

G

G

E

E

K

K

E
x
H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

active site: N165 (= N168), K188 (= K191), E266 (= E268), C300 (= C302), E397 (= E402), E467 (= E482)
binding 4-(diethylamino)benzaldehyde: F166 (= F169), A169 (= A172), V170 (= V173), C300 (= C302), F456 (= F471), H461 (≠ E476)
binding magnesium ion: E119 (= E122), D122 (= D125)

6rttA Piperideine-6-carboxylate dehydrogenase from streptomyces clavuligerus complexed with picolinic acid (see paper)
54% identity, 93% coverage: 35:513/515 of query aligns to 28:507/508 of 6rttA

query
sites
6rttA

N

K

G

G

E

D

I

A

I

L

E

T

S

A

Y

R

S

T

P

P

V

L

D

T

G

G

T

A

L

D

I

L

A

F

K

G

V

L

K

R

A

A

S

Q

T

T

Q

P

E

E

D

D

Y

V

E

D

A

R

A

A

V

V

A

E

K

A

A

A

Q

H

E

T

A

A

F

F

K

L

S

T

W

W

R

R

L

T

V

T

P

P

A

A

P

P

K

V

R

R

G

G

E

A

I

L

V

V

R

K

Q

R

M

F

G

G

E

E

E

L

L

L

R

T

K

E

Y

H

K

K

E

Q

P

D

L

L

G

A

Q

D

L

L

V

V

S

T

Y

I

E

E

M

A

G

G

K

K

S

I

L

R

Q

S

E

E

G

A

L

L

G

G

E

E

V

V

Q

Q

E

E

M

M

I

I

D

D

I

I

C

C

D

D

F

F

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

Y

Y

G

G

L

R

T

T

M

M

H

P

S

T

E

E

R

R

P

P

M

G

H

H

R

R

M

L

Y

M

E

E

Q

T

Y

W

H

H

P

P

V

L

G

G

I

V

V

V

G

G

I

V

I

I

S

S

A
|
A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V
|
V

W

W

S

A

W
|
W

N

N

A

A

M

A

L

V

A

A

W

L

V

V

C

C

G

G

D

D

V

T

C

V

I

V

W

W

K

K

P

P

S

S

E

E

K

L

T

T

P

P

L

L

C

T

G

A

I

L

A

A

C

C

Q

A

N

A

I

L

K

D

T

L

V

A

L

I

E

A

R

D

N

A

N

G

I

A

P

P

E

K

G

G

V

L

S

N

C

Q

L

V

I

V

N

V

G

G

R

A

-

A

D

D

N

V

G

G

D

E

R

R

M

L

N

V

T

D

D

S

K

P

R

R

L

V

P

P

L

L

V

V

S

S

F

A

T

T

G
|
G

S
|
S

T

T

R

R

V

M

G

G

R

R

H

A

V

V

S

G

K

P

T

R

V

V

A

A

E

A

R

R

F

F

G

G

N

R

T

T

I

I

L

L

E
|
E

L

L

G

G

N

N

A

A

I

A

I

V

V

V

S

T

E

P

H

S

A

A

D

D

I

L

N

D

M

L

V

T

L

V

V

N

G

A

A

A

V

V

F

F

G

A

A

A

V

A

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

T

T

T

L

R

R

L

L

I

I

V

V

H

H

E

E

S

D

V

I

Y

A

D

D

K

T

T

V

I

V

E

E

V

R

L

L

K

T

S

A

A

A

Y

F

G

E

Q

R

L

L

K

P

I

I

G

G

N

D

P

P

L

F

D

Q

S

D

N

T

H

L

V

V

G

G

P

P

L

L

I

V

D

N

K

E

D

A

S

A

V

F

Q

G

N

R

Y

M

L

R

D

E

A

A

I

V

E

E

K

R

A

A

K

T

Q

A

E

E

G

G

N

T

V

L

I

C

V

A

E

G

G

G

G

E

V

R

M

Q

Q

F

G

P

E

D

G

A

Y

A

E

P

S

G

G

A

C

Y

Y

Y

V

V

K

R

P

P

C

A

I

L

I

V

E

R

A

M

K

P

N

A

S

Q

F

T

E

A

I

V

V

V

Q

R

H

E

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

L

K

T

Y

Y

N

R

D

D

L

L

E

D

E

E

A

A

I

I

A

R

M

L

Q

N

N

N

D

E

V

V

P
|
P

Q

Q

G

G

L

L

S

S

S

A

S

G

I

I

F

F

T

T

S

A

N

D

M

Q

R

S

E

E

M

A

E

E

L

R

F

F

L

L

S

A

H

P

A

D

G

G

S

A

D

D

C

C

G

G

I

I

A

A

N

N

V

V

N

N

I

I

G

G

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F

F

G

G

E

E

K
|
K

E
|
E

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

R

A

A

Y

Y

M

M

R

R

Q

A

T

T

N

N

T

T

I

V

N

N

Y

Y

G

S

S

G

E

R

L

V

P

T

L

L

A

A

Q

Q

G

G

I

V

R

D

F

F

active site: N161 (= N168), E262 (= E268), C296 (= C302), E476 (= E482)
binding pyridine-2-carboxylic acid: A159 (= A166), F162 (= F169), V166 (= V173), W169 (= W176), G240 (= G246), S241 (= S247), R295 (= R301), C296 (= C302), T297 (= T303), E396 (= E402), F398 (= F404), P421 (= P427), K469 (= K475), E470 (= E476)

6rtsA Piperideine-6-carboxylate dehydrogenase from streptomyces clavuligerus complexed with NAD+ (see paper)
54% identity, 93% coverage: 35:513/515 of query aligns to 29:508/509 of 6rtsA

query
sites
6rtsA

N

K

G

G

E

D

I

A

I

L

E

T

S

A

Y

R

S

T

P

P

V

L

D

T

G

G

T

A

L

D

I

L

A

F

K

G

V

L

K

R

A

A

S

Q

T

T

Q

P

E

E

D

D

Y

V

E

D

A

R

A

A

V

V

A

E

K

A

A

A

Q

H

E

T

A

A

F

F

K

L

S

T

W

W

R

R

L

T

V

T

P

P

A

A

P

P

K

V

R

R

G

G

E

A

I

L

V

V

R

K

Q

R

M

F

G

G

E

E

E

L

L

L

R

T

K

E

Y

H

K

K

E

Q

P

D

L

L

G

A

Q

D

L

L

V

V

S

T

Y

I

E

E

M

A

G

G

K

K

S

I

L

R

Q

S

E

E

G

A

L

L

G

G

E

E

V

V

Q

Q

E

E

M

M

I

I

D

D

I

I

C

C

D

D

F

F

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

Y

Y

G

G

L

R

T

T

M

M

H

P

S

T

E

E

R

R

P

P

M

G

H

H

R

R

M

L

Y

M

E

E

Q

T

Y

W

H

H

P

P

V

L

G

G

I

V

V

V

G

G

I

V

I
|
I

S
|
S

A
|
A

F
|
F

N
|
N

F

F

P

P

V

V

A

A

V

V

W

W

S

A

W

W

N

N

A

A

M

A

L

V

A

A

W

L

V

V

C

C

G

G

D

D

V

T

C

V

I

V

W

W

K
|
K

P

P

S
|
S

E
|
E

K

L

T

T

P

P

L

L

C

T

G

A

I

L

A

A

C

C

Q

A

N

A

I

L

K

D

T

L

V

A

L

I

E

A

R

D

N

A

N

G

I

A

P

P

E

K

G

G

V

L

S

N

C

Q

L

V

I

V

N

V

G

G

R

A

-
x
A

D

D

N

V

G
|
G

D

E

R

R

M

L

N

V

T

D

D

S

K

P

R

R

L

V

P

P

L

L

V

V

S

S

F

A

T
|
T

G
|
G

S
|
S

T

T

R

R

V
x
M

G

G

R

R

H

A

V

V

S

G

K

P

T

R

V

V

A

A

E

A

R

R

F

F

G

G

N

R

T

T

I

I

L

L

E
|
E

L
|
L

G

G

N

N

A

A

I

A

I

V

V

V

S

T

E

P

H

S

A

A

D

D

I

L

N

D

M

L

V

T

L

V

V

N

G

A

A

A

V

V

F

F

G

A

A

A

V

A

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

T

L

R

R

L

L

I

I

V

V

H

H

E

E

S

D

V

I

Y

A

D

D

K

T

T

V

I

V

E

E

V

R

L

L

K

T

S

A

A

A

Y

F

G

E

Q

R

L

L

K

P

I

I

G

G

N

D

P

P

L

F

D

Q

S

D

N

T

H

L

V

V

G

G

P

P

L

L

I

V

D

N

K

E

D

A

S

A

V

F

Q

G

N

R

Y

M

L

R

D

E

A

A

I

V

E

E

K

R

A

A

K

T

Q

A

E

E

G

G

N

T

V

L

I

C

V

A

E

G

G

G

G

E

V

R

M

Q

Q

F

G

P

E

D

G

A

Y

A

E

P

S

G

G

A

C

Y

Y

Y

V

V

K

R

P

P

C

A

I

L

I

V

E

R

A

M

K

P

N

A

S

Q

F

T

E

A

I

V

V

V

Q

R

H

E

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

L

K

T

Y

Y

N

R

D

D

L

L

E

D

E

E

A

A

I

I

A

R

M

L

Q

N

N

N

D

E

V

V

P

P

Q

Q

G

G

L

L

S

S

S

A

S

G

I

I

F

F

T

T

S

A

N

D

M

Q

R

S

E

E

M

A

E

E

L

R

F

F

L

L

S

A

H

P

A

D

G

G

S

A

D

D

C

C

G

G

I

I

A

A

N

N

V

V

N

N

I

I

G

G

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F

F

G

G

E

E

K

K

E

E

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

R

A

A

Y

Y

M

M

R

R

Q

A

T

T

N

N

T

T

I

V

N

N

Y

Y

G

S

S

G

E

R

L

V

P

T

L

L

A

A

Q

Q

G

G

I

V

R

D

F

F

active site: N162 (= N168), E263 (= E268), C297 (= C302), E477 (= E482)
binding nicotinamide-adenine-dinucleotide: I158 (= I164), S159 (= S165), A160 (= A166), F161 (= F167), N162 (= N168), K185 (= K191), S187 (= S193), E188 (= E194), A222 (vs. gap), G225 (= G230), T240 (= T245), G241 (= G246), S242 (= S247), M245 (≠ V250), E263 (= E268), L264 (= L269), C297 (= C302), E397 (= E402), F399 (= F404)

6rtuA Piperideine-6-carboxylate dehydrogenase from streptomyces clavuligerus complexed with alpha-aminoadipic acid (see paper)
53% identity, 93% coverage: 35:513/515 of query aligns to 28:504/505 of 6rtuA

query
sites
6rtuA

N

K

G

G

E

D

I

A

I

L

E

T

S

A

Y

R

S

T

P

P

V

L

D

T

G

G

T

A

L

D

I

L

A

F

K

G

V

L

K

R

A

A

S

Q

T

T

Q

P

E

E

D

D

Y

V

E

D

A

R

A

A

V

V

A

E

K

A

A

A

Q

H

E

T

A

A

F

F

K

L

S

T

W

W

R

R

L

T

V

T

P

P

A

A

P

P

K

V

R

R

G

G

E

A

I

L

V

V

R

K

Q

R

M

F

G

G

E

E

E

L

L

L

R

T

K

E

Y

H

K

K

E

Q

P

D

L

L

G

A

Q

D

L

L

V

V

S

T

Y

I

E

E

M

A

G

G

K

K

S

I

L

R

Q

S

E

E

G

A

L

L

G

G

E

E

V

V

Q

Q

E
|
E

M

M

I

I

D

D

I

I

C

C

D

D

F

F

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

Y

Y

G

G

L

R

T

T

M

M

H

P

S

T

E

E

R

R

P

P

M

G

H

H

R

R

M

L

Y

M

E

E

Q

T

Y

W

H

H

P

P

V

L

G

G

I

V

V

V

G

G

I

V

I

I

S

S

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

W

S

A

W

W

N

N

A

A

M

A

L

V

A

A

W

L

V

V

C

C

G

G

D

D

V

T

C

V

I

V

W

W

K

K

P

P

S

S

E

E

K

L

T

T

P

P

L

L

C

T

G

A

I

L

A

A

C

C

Q

A

N

A

I

L

K

D

T

L

V

A

L

I

E

A

R

D

N

A

N

G

I

A

P

P

E

K

G

G

V

L

S

N

C

Q

L

V

I

V

N

V

G

G

R

A

-

A

D

D

N

V

G

G

D

E

R

R

M

L

N

V

T

D

D

S

K

P

R

R

L

V

P

P

L

L

V

V

S

S

F

A

T

T

G

G

S

S

T

-

R

-

V

-

G

G

R

R

H

A

V

V

S

G

K

P

T

R

V

V

A

A

E

A

R

R

F

F

G

G

N

R

T

T

I

I

L

L

E
|
E

L

L

G

G

N

N

A

A

I

A

I

V

V

V

S

T

E

P

H

S

A

A

D

D

I

L

N

D

M

L

V

T

L

V

V

N

G

A

A

A

V

V

F

F

G

A

A

A

V

A

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

T

T

T

L

R

R

L

L

I

I

V

V

H

H

E

E

S

D

V

I

Y

A

D

D

K

T

T

V

I

V

E

E

V

R

L

L

K

T

S

A

A

A

Y

F

G

E

Q

R

L

L

K

P

I

I

G

G

N

D

P

P

L

F

D

Q

S

D

N

T

H

L

V

V

G

G

P

P

L

L

I

V

D

N

K

E

D

A

S

A

V

F

Q

G

N

R

Y

M

L

R

D

E

A

A

I

V

E

E

K

R

A

A

K

T

Q

A

E

E

G

G

N

T

V

L

I

C

V

A

E

G

G

G

G

E

V

R

M

Q

Q

F

G

P

E

D

G

A

Y

A

E

P

S

G

G

A

C

Y

Y

Y

V

V

K

R

P

P

C

A

I

L

I

V

E

R

A

M

K

P

N

A

S

Q

F

T

E

A

I

V

V

V

Q

R

H

E

E

E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

V

V

M

L

K

T

Y

Y

N

R

D

D

L

L

E

D

E

E

A

A

I

I

A

R

M

L

Q

N

N

N

D

E

V

V

P

P

Q

Q

G

G

L

L

S

S

S

A

S

G

I

I

F

F

T

T

S

A

N

D

M

Q

R

S

E

E

M

A

E

E

L

R

F

F

L

L

S

A

H

P

A

D

G

G

S

A

D

D

C

C

G

G

I

I

A

A

N

N

V

V

N

N

I

I

G

G

T

T

S
|
S

G
|
G

A
|
A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

E

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

K

R

A

A

Y

Y

M

M

R

R

Q

A

T

T

N

N

T

T

I

V

N

N

Y

Y

G

S

S

G

E

R

L

V

P

T

L

L

A

A

Q

Q

G

G

I

V

R

D

F

F

active site: N161 (= N168), E259 (= E268), C293 (= C302), E473 (= E482)
binding 2-aminohexanedioic acid: E115 (= E122), F162 (= F169), R292 (= R301), C293 (= C302), T294 (= T303), S454 (= S463), G455 (= G464), A456 (= A465), F462 (= F471)

6fkuA Structure and function of aldehyde dehydrogenase from thermus thermophilus: an enzyme with an evolutionarily-distinct c-terminal arm (recombinant protein with shortened c-terminal, in complex with NADP) (see paper)
31% identity, 94% coverage: 25:509/515 of query aligns to 15:510/511 of 6fkuA

query
sites
6fkuA

G

G

T

H

S

L

T

V

G

G

S

G

N

E

S

E

F

V

A

L

N

E

G

G

E

P

I

L

E

E

S

R

Y

R

S

N

P

P

V

S

D

D

G

R

-

E

T

D

L

V

I

V

A

A

K

R

V

F

K

P

A

E

S

A

T

D

Q

K

E

D

D

L

Y

V

E

R

A

K

A

A

V

A

A

L

K

K

A

A

Q

R

E

E

A

A

F

F

K

A

S

E

W

W

R

S

L

R

V

T

P

P

A

A

P

P

K

I

R

R

G

G

E

Q

I

V

V

L

R

F

Q

N

M

L

G

V

E

K

E

I

L

L

R

E

K

R

Y

E

K

K

E

P

P

T

L

L

G

T

Q

R

L

L

V

M

S

V

Y

R

E

E

M

V

G

G

K

K

S

T

L

P

Q

K

E

E

G

A

L

A

G

G

E

D

V

V

Q

Q

E

E

M

A

I

I

D

D

I

T

C

A

D

L

F

F

A

F

V

A

G

S

L

E

S

G

R

R

Q

R

L

L

Y

Y

G

G

L

Q

T

T

M

V

H

P

S

S

E

E

R

M

P

R

M

D

H

K

R

E

M

L

Y

F

E

T

Q

F

Y

R

H

R

P

P

V

L

G

G

I

V

V

V

G

G

I

I

I
|
I

S
x
T

A

A

F

G

N
|
N

F

F

P

P

V

I

A

A

V

V

W

P

S

S

W

W

N

K

A

L

M

I

L

P

A

A

W

V

V

L

C

T

G

G

D

N

V

T

C

V

I

V

W

W

K
|
K

P

P

S
|
S

E
|
E

K

D

T

A

P

P

L

T

C

L

G

S

I

F

A

-

C

-

Q

-

N

-

I

V

I

F

K

A

T

K

V

L

L

F

E

E

R

E

N

A

N

G

I

L

P

P

E

P

G

G

V

V

S

L

C

N

L

V

I

V

N

F

G

G

R
x
G

D
x
G

N
x
K

G

G

D

S

-

T

-
x
G

-
x
Q

-

W

-

M

-

V

R

E

M

L

N

M

T

D

D

E

K

G

R

L

L

F

P

Q

L

K

V

F

S

A

F
|
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

V
|
V

G

G

R

R

H

W

V

I

S

G

K

E

T

V

V

A

A

G

E

R

R

N

F

L

G

I

N

R

T

P

I

T

L

L

E
|
E

L
|
L

G

G

N

K

N

N

A

P

I

L

I

V

V

V

S

M

E

R

H

D

A

A

D

D

I

L

N

D

M

L

V

A

L

V

V

E

G

G

A

A

V

W

F

W

G

S

A

A

V

F

G

A

T

T

A

G

G

G

Q

Q

R

R

C
|
C

T

T

T

S

T

A

R

G

R

N

L

I

I

L

V

V

H

D

E

A

S

P

V

I

Y

Y

D

E

K

E

T

F

I

K

E

R

V

R

L

F

K

L

S

E

A

R

Y

V

G

E

Q

A

L

T

K

L

I

V

G

G

N

N

P

P

-

L

L

L

D

H

S

P

N

E

N

V

H

T

V

Y

G

G

P

P

L

F

I

I

D

N

K

E

D
x
R

S
x
F

V

F

Q

A

N

R

Y

W

L

Q

D

E

A

H

I

Y

E

R

K

V

A

G

K

E

Q

A

E

E

G

G

G

A

N

R

V

L

I

L

V

F

-

G

E

R

G

G

G

R

V

I

M

T

Q

R

G

E

E

N

G

P

Y

Y

-

P

-

R

-

F

-

L

-

G

-

D

-

P

E

E

S

A

G

G

C

L

Y

Y

V

G

K

W

P

P

C

T

I

V

I

W

E

E

A

V

K

R

N

P

S

G

F

T

E

R

I

L

V

F

Q

T

H

E

E
|
E

T

V

F
|
F

A

G

P

P

I

T

L

I

Y

N

V

L

M

V

K

K

Y

V

N

D

D

G

L

I

E

E

A

A

I

I

A

A

M

V

Q

A

N

N

D

S

V

T

P

P

Q

Y

G

G

L

L

S

S

S

A

I

I

F

Y

T

T

S

N

N

H

M

R

R

H

E

W

M

A

E

Y

L

L

F

F

L

-

S

-

H

K

A

V

G

G

S

I

D

R

C

A

G

G

I

M

A

T

N

S

V

I

N

N

I

N

G

A

T

T

S

V

G

G

A

A

E

E

I

A

G

H

G

L

A

P

F

F

G

G

E

V

K

K

E

A

T

S

G

G

-

N

G

G

R

R

E
|
E

S

S

G

G

S

I

D

W

A

V

W

L

K

E

A

E

Y

Y

M

T

R

Y

R

W

Q

H

T

A

N

V

T

N

I

E

N

E

Y

Y

G

S

S

G

E

R

L

L

P

Q

L

L

A

A

Q

Q

active site: N159 (= N168), E261 (= E268), C295 (= C302), E483 (= E482)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I155 (= I164), T156 (≠ S165), N159 (= N168), K182 (= K191), S184 (= S193), E185 (= E194), G214 (≠ R227), G215 (≠ D228), K216 (≠ N229), G220 (vs. gap), Q221 (vs. gap), F237 (= F244), T238 (= T245), G239 (= G246), S240 (= S247), V243 (= V250), E261 (= E268), L262 (= L269), C295 (= C302), R342 (≠ D348), F343 (≠ S349), E404 (= E402), F406 (= F404)

4o6rA Crystal structure of a putative aldehyde dehydrogenase from burkholderia cenocepacia
29% identity, 91% coverage: 39:506/515 of query aligns to 21:484/489 of 4o6rA

query
sites
4o6rA

I

I

E

D

S

V

Y

L

S

N

P

P

V

H

D

D

G

G

T

S

L

V

I

I

A

T

K

K

V

I

K

A

A

A

S

A

T

T

Q

A

E

A

D

D

Y

V

E

D

A

L

A

A

V

V

A

D

K

A

A

A

Q

T

E

R

A

A

F

F

K

P

S

A

W

W

R

S

L

A

V

M

P

P

A

A

P

A

K

E

R

R

G

G

E

R

I

L

V

L

R

L

Q

R

M

L

G

A

E

D

E

A

L

I

R

E

K

A

Y

N

K

T

E

E

P

A

L

L

G

A

Q

Q

L

L

V

E

S

S

Y

L

E

D

M

T

G

G

K

H

S

P

L

I

Q

R

E

D

G

S

L

R

G

A

-

L

E

D

V

V

Q

P

E

R

M

T

I

A

D

A

I

C

C

F

D

R

F

Y

A

F

V

G

G

G

L

M

S

A

R

D

Q

K

L

L

Y

Q

G

G

L

S

T

V

M

I

H

P

S

V

E

D

R

T

P

G

M

F

H

L

R

N

M

-

Y

Y

E

V

Q

Q

Y

R

H

A

P

P

V

I

G

G

I

V

V

V

G

G

I

Q

I
|
I

S
x
V

A

P

F

W

N
|
N

F

F

P

P

V

L

A

M

V

F

W

T

S

S

W

W

N

K

A

M

M

G

L

P

A

A

W

L

V

A

C

A

G

G

D

N

V

T

C

V

I

V

W

L

K
|
K

P

P

S

S

E

E

K

I

T

T

P

P

L

L

C

S

G

T

I

L

A

R

C

I

Q

V

N

E

I

L

I

M

K

A

T

E

V

V

L

-

E

-

R

-

N

-

N

G

I

F

P

P

E

A

G

G

V

V

S

V

C

N

L

I

I

V

N

P

G

G

R

Y

D
x
G

N

H

-

T

-

A

G
|
G

D
x
Q

R

R

M

L

N

A

T

E

D

H

K

P

R

G

L

V

P

G

L

K

V

I

S

A

F
|
F

T

T

G
|
G

S
|
S

T

T

R

A

V
x
T

G

G

R

R

H
x
R

V

I

S

V

K

E

T

A

V

S

A

Q

E

G

R

N

F

L

G

K

N

R

T

V

I

Q

L

L

E
|
E

L

L

G

G

N

K

N

G

A

A

I

N

I

I

V

V

S

F

E

D

H

D

A

A

D

N

I

L

N

D

M

A

V

A

L

I

V

N

G

G

A

A

V

A

F

W

G

A

A

I

V

F

G

H

T

N

A

Q

G

G

Q

Q

R

A

C
|
C

T

I

T

A

T

G

R

S

R

R

L

L

I

V

V

L

H

H

E

E

S

R

V

I

Y

A

D

D

K

A

T

F

I

L

E

E

V

R

L

F

K

V

S

A

A

L

Y

A

G

S

Q

S

L

I

K

R

I

I

G

G

N

N

P

P

L

L

D

D

S

P

N

N

N

T

H

E

V

M

G

G

P

P

L

L

I

T

D

S

K

K

D

Q

S

H

V

L

Q

D

N

R

Y

V

L

L

D

S

A

Y

I

V

E

D

K

V

A

A

K

R

Q

E

E

Q

G

G

N

R

V

V

I

L

V

T

E

G

G

G

G

S

V

A

M

P

Q

Q

G

D

E

P

G

A

Y

L

E

A

S

N

G

G

C

Y

Y

Y

V

V

K

R

P

P

C

T

I

I

I

V

E

E

A

A

K

K

N

H

S

A

F

T

E

D

-

R

I

V

V

A

Q

Q

H

E

E
|
E

T

V

F

F

A

G

P

P

I

F

L

V

Y

T

V

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M

L

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F

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G

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S

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D

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D

E

E

A

A

I

L

A

A

M

I

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A

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N

D

A

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T

P

E

Q

Y

G

G

L

L

S

G

S

S

S

G

I

L

F

W

T

T

S

N

N

D

M

L

R

S

E

R

M

A

E

H

L

R

F

M

L

A

S

A

H

R

A

I

G

-

S

-

D

D

C

A

G

G

I

M

A

C

N

W

V

I

N

N

I

C

G

-

T

Y

S

K

G

R

A

V

E

N

I

P

G

G

G

S

A

P

F

F

G

G

E

V

K

G

E

K

T

S

G

G

G

Y

G

G

R

R

E
|
E

S

M

G

G

S

F

D

E

A

A

W

M

K

H

A

D

Y

Y

M

T

R

E

R

A

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R

T

S

N

V

T

W

I

V

N

N

Y

V

G

D

S

G

E

N

L

V

P

P

active site: N150 (= N168), K173 (= K191), E248 (= E268), C282 (= C302), E383 (= E402), E460 (= E482)
binding adenosine monophosphate: I146 (= I164), V147 (≠ S165), K173 (= K191), G206 (≠ D228), G210 (= G230), Q211 (≠ D231), F224 (= F244), G226 (= G246), S227 (= S247), T230 (≠ V250), R233 (≠ H253)

6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
30% identity, 91% coverage: 34:501/515 of query aligns to 14:477/477 of 6j76A

query
sites
6j76A

A

S

N

N

G

G

E

Q

I

T

I

E

E

P

S

V

Y

V

S

S

P

P

V

I

D

N

G

E

T

E

L

V

I

L

A

A

K

Y

V

I

K

Q

A

D

S

A

T

D

Q

A

E

S

D

D

Y

A

E

E

A

R

A

V

V

L

A

A

K

V

A

A

Q

T

E

T

A

A

F

Q

K

K

S

E

W

W

R

A

L

K

V

Q

P

P

A

A

P

R

K

Q

R

R

G

A

E

E

I

V

V

L

R

R

Q

K

M

F

G

A

E

Q

E

L

L

I

R

R

K

D

Y

N

K

K

E

Q

P

Y

L

L

G

A

Q

E

L

L

V

L

S

V

Y

K

E

E

M

Q

G

G

K

K

S

L

L

L

Q

K

E

V

G

A

L

L

G

G

E

E

V

V

Q

E

E

A

M

T

I

S

D

T

I

F

C

I

D

E

F

Y

A

A

V

C

G

D

L

W

S

A

R

R

Q

Q

L

M

Y

D

G

G

L

D

T

I

M

V

H

K

S

S

E

D

R

N

P

A

M

N

H

E

R

Q

M

I

Y

M

E

I

Q

H

Y

K

H

I

P

P

V

R

G

G

I

V

V

V

G

V

I

A

I
|
I

S
x
T

A
|
A

F
x
W

N
|
N

F

F

P

P

V

L

A

A

V

L

W

A

S

G

W

R

N

K

A

I

M

G

L

P

A

A

W

L

V

V

C

A

G

G

D

N

V

S

C

I

I

V

W

V

K
|
K

P

P

S
x
T

E
x
S

K

E

T

T

P

P

L

L

C

A

G

T

I

L

A

E

C

-

Q

-

N

-

I

-

I

L

K

G

T

Y

V

L

L

A

E

E

R

Q

N

A

N

G

I

I

P

P

E

A

G

G

V

V

S

L

C

N

L

I

I

V

N

T

G

G

-

G

-
x
G

R

R

D

T

N

L

G
|
G

D

N

R

E

M

L

N

V

T

G

D

H

K

R

R

M

L

T

P

N

L

M

V

V

S

S

F

M

T
|
T

G
|
G

S
|
S

T

T

R

P

V
x
A

G

G

R

Q

H
x
S

V
x
I

S

I

K

R

T

A

V

S

A

A

E

N

R

N

F

M

G

A

N

H

T

V

I

Q

L

L

E
|
E

L
|
L

G

G

N

K

N

A

A

P

I

F

I

I

V

V

S

M

E

E

H

D

A

A

D

D

I

L

N

E

M

Q

V

A

L

A

V

A

G

A

A

A

V

L

F

H

G

S

A

R

V

F

G

D

T

N

A

C

G

G

Q

Q

R

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C
|
C

T

T

T

C

T

N

R

E

R

R

L

M

I

Y

V

V

H

H

E

G

S

A

V

V

Y

Y

D

D

K

E

T

F

I

M

E

R

V

I

L

F

K

M

S

G

A

K

Y

V

G

E

Q

A

L

I

K

K

I

V

G

G

N

D

P

P

L

M

D

D

S

P

N

A

N

S

H

D

V

M

G

G

P

P

L

K

I

V

D

N

K

A

D

N

S

E

V

L

Q

A

N

H

Y

M

L

E

D

E

A

L

I

V

E

A

K

E

A

A

K

V

Q

D

E

E

G

G

G

A

N

T

V

V

I

L

V

F

E

G

G

G

G

K

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K

M

L

Q

E

G

G

E

P

G

E

Y

F

E

E

S

K

G

G

C

F

Y

W

V

F

K

E

P

P

C

T

I

V

I

L

-

T

E

N

A

V

K

T

N

Q

S

D

F

M

E

T

I

I

V

V

Q

H

H

E

E
|
E

T

S

F
|
F

A

G

P

P

I

I

L

L

Y

P

V

V

M

I

K

K

Y

F

N

D

D

S

L

F

E

D

E

E

A

V

I

I

A

E

M

Y

Q

A

N

N

D

D

V

S

P

D

Q

Y

G

G

L

L

S

A

S

M

I

I

F

C

T

T

S

Q

N

N

M

M

R

H

E

Y

M

I

E

N

L

R

F

L

L

L

S

T

H

E

A

L

G

E

S

S

D

-

C

-

G

G

I

E

A

I

N

Y

V

V

N

N

I

R

G

G

T

-

S

H

G

G

A

E

E

Q

I

H

G

Q

G

G

A

F

F
x
H

G

N

G

G

E

Y

K

K

E

L

T

S

G

G

G

T

G

G

R

G

E
|
E

S

D

G

G

S

K

D

Y

A

G

W

F

K

E

A

Q

Y

Y

M

L

R

E

R

K

Q

K

T

T

N

F

T

Y

I

I

N

N

Y

Y

active site: N148 (= N168), E246 (= E268), C280 (= C302), E458 (= E482)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I144 (= I164), T145 (≠ S165), A146 (= A166), W147 (≠ F167), N148 (= N168), K171 (= K191), T173 (≠ S193), S174 (≠ E194), G204 (vs. gap), G208 (= G230), T223 (= T245), G224 (= G246), S225 (= S247), A228 (≠ V250), S231 (≠ H253), I232 (≠ V254), E246 (= E268), L247 (= L269), C280 (= C302), E381 (= E402), F383 (= F404), H447 (≠ F471)

P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
30% identity, 91% coverage: 32:501/515 of query aligns to 9:481/487 of P42412

query
sites
P42412

S

N

F

Y

A

I

N

N

G

G

E

E

I

W

I

V

E

E

S

S

Y

K

S

T

-

D

-

Q

-

Y

-

E

-

D

-

V

-

N

P

P

V

A

D

T

G

K

T

E

L

V

I

L

A
x
C

K

Q

V

V

K

P

A

I

S

S

T

T

Q

K

E

E

D

D

Y

I

E

D

A

Y

A

A

V

A

A

Q

K

T

A

A

Q

A

E

E

A

A

F

F

K

K

S

T

W

W

R

S

L

K

V

V

P

A

A

V

P

P

K

R

R

R

G

A

E

R

I

I

V

L

R

F

Q

N

M

F

G

Q

E

Q

E

L

L

L

R

S

K

Q

Y

H

K

K

E

E

P

E

L

L

G

A

Q

H

L

L

V

I

S

T

Y

I

E

E

M

N

G

G

K

K

S

N

L

T

Q

K

E

E

G

A

L

L

G

G

E

E

V

V

Q

G

E
x
R

M

G

I

I

D

E

I

N

C

V

D

E

F

F

A

A

V

A

G

G

L

A

S

P

R

S

Q

L

L

M

Y

M

G

G

L

D

T

S

M

L

H

A

S

S

E

I

R

A

P

T

M

D

H

V

R

E

M

A

Y

A

E

N

Q

Y

Y

R

H

Y

P

P

V

I

G

G

I

V

V

V

G

G

I

G

I

I

S
x
A

A

P

F
|
F

N

N

F

F

P

P

V

M

A

M

V

V

W

P

S
x
C

W

W

N

M

A

F

M

P

L

M

A

A

W

I

V

A

C

L

G

G

D

N

V

T

C

F

I

I

W

L

K
|
K

P

P

S

S

E
|
E

K
x
R

T

T

P

P

L

L

C

L

G

-

I

-

A

-

C

T

Q

E

N

K

I

L

I

V

K

E

T

-

V

L

L

F

E

E

R

K

N

A

N

G

I

L

P

P

E

K

G

G

V

V

S

F

C

N

L

V

I

V

N

Y

G

G

-

A

R

H

D

D

N

V

G

V

D

N

R

G

M

I

N

L

T

E

D

H

K

P

R

E

L

I

P

K

L

A

V

I

S

S

F

F

T

V

G

G

S
|
S

T

K

R

P

V

V

G

G

R

E

H

Y

V

V

S

Y

K

K

T

K

V

G

A

S

E

E

R

N

F

L

G

K

N

R

T

V

I

Q

L

S

E

L

L
x
T

G

G

G

A

N

K

N

N

A

H

I

T

I

I

V

V

S

L

E

N

H

D

A

A

D

N

I

L

N

E

M

D

V

T

L

V

V

T

G

N

A

I

V

V

F

G

G

A

A

A

V

F

G

G

T

S

A

A

G

G

Q

E

R
|
R

C

C

T

M

T

A

T
x
C

R

A

R

V

L

V

I

T

V

V

H

E

E

E

S

G

V

I

Y

A

D

D

K

E

T

F

I

M

E

A

V

K

L

L

K

Q

S

E

A

K

Y

V

G

A

Q

D

L

I

K

K

I

I

G

G

N

N

P

G

L

L

D

D

S

D

N

G

N

V

H

F

V

L

G

G

P

P

L

V

I

I

D

R

K

E

D

D

S

N

V

K

Q

K

N

R

Y

T

L

L

D

S

A

Y

I

I

E

E

K

K

A

G

K

L

Q

E

E

E

G

G

G

A

N

R

V

L

I

V

V
x
C

E

D

G

G

G

-

V

-

M

-

Q

R

G

E

E

N

G

V

Y

S

E

D

S

D

G

G

C

Y

Y

F

V

V

K

G

P

P

C

T

I

I

I

F

E

D

-

N

A

V

K

T

N

T

S

E

F

M

E

T

I

I

V

W

Q

K

H

D

E
|
E

T

I

F

F

A

A

P

P

I

V

L

L

Y

S

V

V

M

I

K

R

Y

V

N

K

D

N

L

L

E

K

E

E

A

A

I

I

A

E

M

I

Q

A

N

N

D

K

V

S

P

E

Q

F

G

A

L

N

S

G

S

A

S
x
C

I

L

F

F

T

T

S

S

N

N

M

S

R

N

E

A

M

I

E

R

L

Y

F

F

L

R

S

E

H

N

A

I

G

-

S

-

D

D

C

A

G

G

I

M

A

L

N

G

V

I

N

N

I

L

G

G

T

V

S

P

G

A

A

P

E

M

I

A

G

F

A

P

F

F

G

S

G

G

E

W

K

K

E

S

T

S

-

F

G

G

G

T

G

L

R

H

E

A

S

N

G

G

S

K

D

D

A

S

W

V

K

D

A

F

Y

Y

M

T

R

R

R

K

Q

K

T

V

N

V

T

T

I

A

N

R

Y

Y

C36 (≠ A51) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
R107 (≠ E122) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
A150 (≠ S165) binding NAD(+)
F152 (= F167) binding NAD(+)
C160 (≠ S175) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
K176 (= K191) binding NAD(+)
E179 (= E194) binding NAD(+)
R180 (≠ K195) binding NAD(+)
S229 (= S247) binding NAD(+)
T251 (≠ L269) binding NAD(+)
R283 (= R301) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
C287 (≠ T305) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
C351 (≠ V369) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
E382 (= E402) binding NAD(+)
C413 (≠ S433) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.

1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
30% identity, 91% coverage: 32:501/515 of query aligns to 7:479/484 of 1t90A

query
sites
1t90A

S

N

F

Y

A

I

N

N

G

G

E

E

I

W

I

V

E

E

S

S

Y

K

S

T

-

D

-

Q

-

Y

-

E

-

D

-

V

-

N

P

P

V

A

D

T

G

K

T

E

L

V

I

L

A

C

K

Q

V

V

K

P

A

I

S

S

T

T

Q

K

E

E

D

D

Y

I

E

D

A

Y

A

A

V

A

A

Q

K

T

A

A

Q

A

E

E

A

A

F

F

K

K

S

T

W

W

R

S

L

K

V

V

P

A

A

V

P

P

K

R

R

R

G

A

E

R

I

I

V

L

R

F

Q

N

M

F

G

Q

E

Q

E

L

L

L

R

S

K

Q

Y

H

K

K

E

E

P

E

L

L

G

A

Q

H

L

L

V

I

S

T

Y

I

E

E

M

N

G

G

K

K

S

N

L

T

Q

K

E

E

G

A

L

L

G

G

E

E

V

V

Q

G

E

R

M

G

I

I

D

E

I

N

C

V

D

E

F

F

A

A

V

A

G

G

L

A

S

P

R

S

Q

L

L

M

Y

M

G

G

L

D

T

S

M

L

H

A

S

S

E

I

R

A

P

T

M

D

H

V

R

E

M

A

Y

A

E

N

Q

Y

Y

R

H

Y

P

P

V

I

G

G

I

V

V

V

G

G

I

G

I
|
I

S
x
A

A
x
P

F
|
F

N
|
N

F

F

P

P

V

M

A

M

V

V

W

P

S

C

W
|
W

N

M

A

F

M

P

L

M

A

A

W

I

V

A

C

L

G

G

D

N

V

T

C

F

I

I

W

L

K
|
K

P

P

S

S

E
|
E

K
x
R

T

T

P

P

L

L

C

L

G

-

I

-

A

-

C

T

Q

E

N

K

I

L

I

V

K

E

T

-

V

L

L

F

E

E

R

K

N

A

N

G

I

L

P

P

E

K

G

G

V

V

S

F

C

N

L

V

I

V

N

Y

G

G

-

A

R
x
H

D

D

N

V

G

V

D

N

R

G

M

I

N

L

T

E

D

H

K

P

R

E

L

I

P

K

L

A

V

I

S

S

F

F

T
x
V

G
|
G

S
|
S

T

K

R

P

V
|
V

G

G

R

E

H

Y

V

V

S

Y

K

K

T

K

V

G

A

S

E

E

R

N

F

L

G

K

N

R

T

V

I

Q

L

S

E
x
L

L
x
T

G

G

G

A

N

K

N

N

A

H

I

T

I

I

V

V

S

L

E

N

H

D

A

A

D

N

I

L

N

E

M

D

V

T

L

V

V

T

G

N

A

I

V

V

F

G

G

A

A

A

V

F

G

G

T

S

A

A

G

G

Q

E

R

R

C
|
C

T

M

T

A

T

C

R

A

R

V

L

V

I

T

V

V

H

E

E

E

S

G

V

I

Y

A

D

D

K

E

T

F

I

M

E

A

V

K

L

L

K

Q

S

E

A

K

Y

V

G

A

Q

D

L

I

K

K

I

I

G

G

N

N

P

G

L

L

D

D

S

D

N

G

N

V

H

F

V

L

G

G

P

P

L

V

I

I

D

R

K

E

D

D

S

N

V

K

Q

K

N

R

Y

T

L

L

D

S

A

Y

I

I

E

E

K

K

A

G

K

L

Q

E

E

E

G

G

G

A

N

R

V

L

I

V

V

C

E

D

G

G

G

-

V

-

M

-

Q

R

G

E

E

N

G

V

Y

S

E

D

S

D

G

G

C

Y

Y

F

V

V

K

G

P

P

C

T

I

I

I

F

E

D

-

N

A

V

K

T

N

T

S

E

F

M

E

T

I

I

V

W

Q

K

H

D

E
|
E

T

I

F
|
F

A

A

P

P

I

V

L

L

Y

S

V

V

M

I

K

R

Y

V

N

K

D

N

L

L

E

K

E

E

A

A

I

I

A

E

M

I

Q

A

N

N

D

K

V

S

P

E

Q

F

G

A

L

N

S

G

S

A

S

C

I

L

F

F

T

T

S

S

N

N

M

S

R

N

E

A

M

I

E

R

L

Y

F

F

L

R

S

E

H

N

A

I

G

-

S

-

D

D

C

A

G

G

I

M

A

L

N

G

V

I

N

N

I

L

G

G

T

V

S

P

G

A

A

P

E

M

I

A

G

F

A

P

F

F

G

S

G

G

E

W

K

K

E

S

T

S

-

F

G

G

G

T

G

L

R

H

E
x
A

S

N

G

G

S

K

D

D

A

S

W

V

K

D

A

F

Y

Y

M

T

R

R

R

K

Q

K

T

V

N

V

T

T

I

A

N

R

Y

Y

active site: N151 (= N168), K174 (= K191), L248 (≠ E268), C282 (= C302), E380 (= E402), A460 (≠ E482)
binding nicotinamide-adenine-dinucleotide: I147 (= I164), A148 (≠ S165), P149 (≠ A166), F150 (= F167), N151 (= N168), W159 (= W176), K174 (= K191), E177 (= E194), R178 (≠ K195), H207 (≠ R227), V225 (≠ T245), G226 (= G246), S227 (= S247), V230 (= V250), L248 (≠ E268), T249 (≠ L269), C282 (= C302), E380 (= E402), F382 (= F404)

3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
28% identity, 89% coverage: 35:492/515 of query aligns to 23:472/481 of 3jz4A

query
sites
3jz4A

N

N

G

G

E

E

I

A

I

I

E

D

S

V

Y

T

S

N

P

P

V

A

D

N

G

G

T

D

L

K

I

L

A

G

K

S

V

V

K

P

A

K

S

M

T

G

Q

A

E

D

D

E

Y

T

E

R

A

A

V

I

A

D

K

A

A

A

Q

N

E

R

A

A

F

L

K

P

S

A

W

W

R

R

L

A

V

L

P

T

A

A

P

K

K

E

R

R

G

A

E

T

I

I

V

L

R

R

Q

N

M

W

G

F

E

N

E

L

L

M

R

M

K

E

Y

H

K

Q

E

D

P

D

L

L

G

A

Q

R

L

L

V

M

S

T

Y

L

E

E

M

Q

G

G

K

K

S

P

L

L

Q

A

E

E

G

A

L

K

G

G

E

E

V

I

Q

S

E

Y

M

A

I

A

D

S

I

F

C

I

D

E

F

W

A

F

V

A

G

E

L

E

S

G

R

K

Q

R

L

I

Y

Y

G

G

L

D

T

T

M

I

H

P

S

G

E

H

R

Q

P

A

M

D

H

K

R

R

M

L

Y

I

E

V

Q

I

Y

K

H

Q

P

P

V

I

G

G

I

V

V

T

G

A

I

A

I

I

S

T

A
x
P

F
x
W

N
|
N

F

F

P

P

V

A

A

A

V

M

W

I

S

T

W

R

N

K

A

A

M

G

L

P

A

A

W

L

V

A

C

A

G

G

D

C

V

T

C

M

I

V

W

L

K
|
K

P

P

S
x
A

E
x
S

K

Q

T

T

P

P

L

F

C

S

G

A

I

L

A

A

C

-

Q

-

N

-

I

-

I

L

K

A

T

E

V

L

L

A

E

I

R

R

N

A

N

G

I

V

P

P

E

A

G

G

V

V

S

F

C

N

L

V

I

V

N

T

G

G

R

S

D
x
A

N

G

-

A

-

V

G
|
G

D

N

R

E

M

L

N

T

T

S

D

N

K

P

R

L

L

V

P

R

L

K

V

L

S

S

F

F

T

T

G
|
G

S
|
S

T

T

R

E

V
x
I

G

G

R

R

H

Q

V

L

S

M

K

E

T

Q

V

C

A

A

E

K

R

D

F

I

G

K

N

K

T

V

I

S

L

L

E
|
E

L

L

G

G

N

N

N

A

A

P

I

F

I

I

V

V

S

F

E

D

H

D

A

A

D

D

I

L

N

D

M

K

V

A

L

V

V

E

G

G

A

A

V

L

F

A

G

S

A

K

V

F

G

R

T

N

A

A

G

G

Q

Q

R

T

C
|
C

T

V

T

C

T

A

R

N

R

R

L

L

I

Y

V

V

H

Q

E

D

S

G

V

V

Y

Y

D

D

K

R

T

F

I

A

E

E

V

K

L

L

K

Q

S

Q

A

A

Y

M

G

S

Q

K

L

L

K

H

I

I

G

G

N

D

P

G

L

L

D

D

S

N

N

G

N

V

H

T

V

I

G

G

P

P

L

L

I

I

D

D

K

E

D

K

S

A

V

V

Q

A

N
x
K

Y

V

L

E

D

E

A

H

I

I

E

A

K

D

A

A

K

L

Q

E

E

K

G

G

G

A

N

R

V

V

I

V

V

C

E

G

G

G

G

K

V

A

M

H

Q

E

G

-

E

-

G

-

Y

-

E

R

S

G

G

G

C

N

Y

F

V

F

K

Q

P

P

C

T

I

I

-

L

I

V

E

D

A

V

K

P

N

A

S

N

F

A

E

K

I

V

V

S

Q

K

H

E

E
|
E

T

T

F
|
F

A

G

P

P

I

L

L

A

Y

P

V

L

M

F

K

R

Y

F

N

K

D

D

L

E

E

A

E

D

A

V

I

I

A

A

M

Q

Q

A

N

N

D

D

V

T

P

E

Q

F

G

G

L

L

S

A

S

Y

I

F

F

Y

T

A

S

R

N

D

M

L

R

S

E

-

M

-

E

R

L

V

F

F

L

R

S

V

H

G

A

E

G

A

S

L

D

E

C

Y

G

G

I

I

A

V

N

G

V

I

N

N

I

T

G

G

T

I

S

I

G

S

A

N

E

E

I

V

G

-

G

A

A

P

F

F

G

G

E

I

K

K

E

A

T

S

G

G

G

L

G

G

R

R

E
|
E

S

G

G

S

S

K

D

Y

A

G

W

I

K

E

A

D

Y

Y

M

L

active site: N156 (= N168), K179 (= K191), E254 (= E268), C288 (= C302), E385 (= E402), E462 (= E482)
binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (≠ A166), W155 (≠ F167), K179 (= K191), A181 (≠ S193), S182 (≠ E194), A212 (≠ D228), G216 (= G230), G232 (= G246), S233 (= S247), I236 (≠ V250), C288 (= C302), K338 (≠ N352), E385 (= E402), F387 (= F404)

P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
28% identity, 89% coverage: 35:492/515 of query aligns to 24:473/482 of P25526

query
sites
P25526

N

N

G

G

E

E

I

A

I

I

E

D

S

V

Y

T

S

N

P

P

V

A

D

N

G

G

T

D

L

K

I

L

A

G

K

S

V

V

K

P

A

K

S

M

T

G

Q

A

E

D

D

E

Y

T

E

R

A

A

V

I

A

D

K

A

A

A

Q

N

E

R

A

A

F

L

K

P

S

A

W

W

R

R

L

A

V

L

P

T

A

A

P

K

K

E

R

R

G

A

E

T

I

I

V

L

R

R

Q

N

M

W

G

F

E

N

E

L

L

M

R

M

K

E

Y

H

K

Q

E

D

P

D

L

L

G

A

Q

R

L

L

V

M

S

T

Y

L

E

E

M

Q

G

G

K

K

S

P

L

L

Q

A

E

E

G

A

L

K

G

G

E

E

V

I

Q

S

E

Y

M

A

I

A

D

S

I

F

C

I

D

E

F

W

A

F

V

A

G

E

L

E

S

G

R

K

Q

R

L

I

Y

Y

G

G

L

D

T

T

M

I

H

P

S

G

E

H

R

Q

P

A

M

D

H

K

R

R

M

L

Y

I

E

V

Q

I

Y

K

H

Q

P

P

V

I

G

G

I

V

V

T

G

A

I

A

I

I

S

T

A

P

F
x
W

N
|
N

F

F

P

P

V

A

A

A

V

M

W

I

S

T

W

R

N

K

A

A

M

G

L

P

A

A

W

L

V

A

C

A

G

G

D

C

V

T

C

M

I

V

W

L

K
|
K

P
|
P

S
x
A

E
x
S

K

Q

T

T

P

P

L

F

C

S

G

A

I

L

A

A

C

-

Q

-

N

-

I

-

I

L

K

A

T

E

V

L

L

A

E

I

R

R

N

A

N

G

I

V

P

P

E

A

G

G

V

V

S

F

C

N

L

V

I

V

N

T

G

G

R

S

D

A

N

G

-

A

-

V

G

G

D

N

R

E

M

L

N

T

T

S

D

N

K

P

R

L

L

V

P

R

L

K

V

L

S

S

F

F

T

T

G
|
G

S
|
S

T

T

R

E

V

I

G

G

R

R

H

Q

V

L

S

M

K

E

T

Q

V

C

A

A

E

K

R

D

F

I

G

K

N

K

T

V

I

S

L

L

E

E

L
|
L

G

G

N

N

N

A

A

P

I

F

I

I

V

V

S

F

E

D

H

D

A

A

D

D

I

L

N

D

M

K

V

A

L

V

V

E

G

G

A

A

V

L

F

A

G

S

A

K

V

F

G

R

T

N

A

A

G

G

Q

Q

R

T

C

C

T

V

T

C

T

A

R

N

R

R

L

L

I

Y

V

V

H

Q

E

D

S

G

V

V

Y

Y

D

D

K

R

T

F

I

A

E

E

V

K

L

L

K

Q

S

Q

A

A

Y

V

G

S

Q

K

L

L

K

H

I

I

G

G

N

D

P

G

L

L

D

D

S

N

N

G

N

V

H

T

V

I

G

G

P

P

L

L

I

I

D

D

K

E

D

K

S

A

V

V

Q

A

N

K

Y

V

L

E

D

E

A

H

I

I

E

A

K

D

A

A

K

L

Q

E

E

K

G

G

G

A

N

R

V

V

I

V

V

C

E

G

G

G

G

K

V

A

M

H

Q

E

G

-

E

-

G

-

Y

-

E

R

S

G

G

G

C

N

Y

F

V

F

K

Q

P

P

C

T

I

I

-

L

I

V

E

D

A

V

K

P

N

A

S

N

F

A

E

K

I

V

V

S

Q

K

H

E

E
|
E

T

T

F

F

A

G

P

P

I

L

L

A

Y

P

V

L

M

F

K

R

Y

F

N

K

D

D

L

E

E

A

E

D

A

V

I

I

A

A

M

Q

Q

A

N

N

D

D

V

T

P

E

Q

F

G

G

L

L

S

A

S

Y

I

F

F

Y

T

A

S

R

N

D

M

L

R

S

E

-

M

-

E

R

L

V

F

F

L

R

S

V

H

G

A

E

G

A

S

L

D

E

C

Y

G

G

I

I

A

V

N

G

V

I

N

N

I

T

G

G

T

I

S

I

G

S

A

N

E

E

I

V

G

-

G

A

A

P

F

F

G

G

E

I

K

K

E

A

T

S

G

G

G

L

G

G

R

R

E

E

S

G

G

S

S

K

D

Y

A

G

W

I

K

E

A

D

Y

Y

M

L

WN 156:157 (≠ FN 167:168) binding NADP(+)
KPAS 180:183 (≠ KPSE 191:194) binding NADP(+)
GS 233:234 (= GS 246:247) binding NADP(+)
L256 (= L269) binding NADP(+)
E386 (= E402) binding NADP(+)

4neaA 1.90 angstrom resolution crystal structure of betaine aldehyde dehydrogenase (betb) from staphylococcus aureus in complex with NAD+ and bme-free cys289 (see paper)
29% identity, 92% coverage: 33:505/515 of query aligns to 21:499/505 of 4neaA

query
sites
4neaA

F

Y

A

I

N

D

G

G

E

E

I

W

I

V

E

E

S

S

-

A

-

N

-

K

-

N

-

T

-

R

-

D

-

I

Y

I

S

N

P

P

V

Y

D

N

G

Q

T

E

L

V

I

I

A

F

K

T

V

V

K

S

A

E

S

G

T

T

Q

K

E

E

D

D

Y

A

E

E

A

R

A

A

V

I

A

L

K

A

A

A

Q

R

E

R

A

A

F

F

K

E

S

S

-

G

-

E

W

W

R

S

L

Q

V

E

P

T

A

A

P

E

K

T

R

R

G

G

E

K

I

K

V

V

R

R

Q

A

M

I

G

A

E

D

E

K

L

I

R

K

K

E

Y

H

K

R

E

E

P

A

L

L

G

A

Q

R

L

L

V

E

S

T

Y

L

E

D

M

T

G

G

K

K

S

T

L

L

Q

E

E

E

G

S

L

Y

G

A

E

D

V

M

Q

D

E

D

M

I

I

H

D

N

I

V

C

F

D

M

F

Y

A

F

V

A

G

G

L

L

S

A

R

D

Q

K

L

D

Y

G

G

G

L

E

T

M

M

I

H

D

S

S

E

P

R

I

P

P

M

D

H

T

R

E

M

S

Y

K

E

I

Q

V

Y

K

H

E

P

P

V

V

G

G

I

V

V

V

G

T

I

Q

I

I

S

T

A
x
P

F

W

N
|
N

F

Y

P

P

V

L

A

L

V

Q

W

A

S

S

W

W

N

K

A

I

M

A

L

P

A

A

W

L

V

A

C

T

G

G

D

C

V

S

C

L

I

V

W

M

K
|
K

P

P

S

S

E
|
E

K

I

T

T

P

P

L

L

C

T

G

T

I

I

A

R

C

V

Q

F

N

E

I

L

I

M

K

E

T

E

V

V

L

-

E

-

R

-

N

-

N

G

I

F

P

P

E

K

G

G

V

T

S

I

C

N

L

L

I

I

-

L

-

G

N

A

G
|
G

R

S

D

E

N

V

G
|
G

D

D

R

V

M

M

N

S

T

G

D

H

K

K

R

E

L

V

P

D

L

L

V

V

S

S

F

F

T

T

G
|
G

S
x
G

T

I

R

E

V
x
T

G

G

R

K

H
|
H

V
x
I

S

M

K

K

T

N

V

A

A

A

E

N

R

N

F

V

G

T

N

N

T

I

I

A

L

L

E
|
E

L

L

G

G

N

K

N

N

A

P

I

N

I

I

V

I

S

F

E

D

H

D

A

A

D

D

I

F

N

E

M

L

V

A

L

V

V

D

G

Q

A

A

V

L

F

N

G

G

A

G

V

Y

G

F

T

H

A

A

G

G

Q

Q

R

V

C
|
C

T

S

T

A

T

G

R

S

R

R

L

I

I

L

V

V

H

Q

E

N

S

S

V

I

Y

K

D

D

K

K

T

F

I

E

E

Q

V

A

L

L

K

I

S

D

A

R

Y

V

G

K

Q

K

L

I

K

K

I

L

G

G

N

N

P

G

L

F

D

D

S

A

N

D

N

T

H

E

V

M

G

G

P

P

L

V

I

I

D

S

-

T

-

E

-

H

K

R

D

N

S

K

V

I

Q

E

N

S

Y

Y

L

M

D

D

A

V

I

-

E

-

K

-

A

A

K

K

Q

A

E

E

G

G

G

A

N

T

V

I

I

A

V

V

E

G

G

G

G

K

V

R

M

P

Q

D

G

R

E

D

G

D

Y

L

E

K

S

D

G

G

C

L

Y

F

V

F

K

E

P

P

C

T

I

V

I

I

-

T

E

N

A

C

K

D

N

T

S

S

F

M

E

R

I

I

V

V

Q

Q

H

E

E
|
E

T

V

F
|
F

A

G

P

P

I

V

L

V

Y

T

V

V

M

E

K

G

Y

F

N

E

D

T

L

E

E

Q

E

E

A

A

I

I

A

Q

M

L

Q

A

N

N

D

D

V

S

P

I

Q

Y

G

G

L

L

S

A

S

G

S

A

I

V

F

F

T

S

S

K

N

D

M

I

R

G

E

K

M

A

E

Q

L

-

F

-

L

-

S

-

H

R

A

V

G

A

S

N

D

K

C

L

G

K

I

L

A

G

N

T

V

V

N

W

I

I

G

N

T

D

S

F

G

H

A

P

E

Y

I

F

G

A

G

Q

A

A

-

P

F

W

G

G

E

Y

K

K

E

Q

T

S

G

G

G

I

G

G

R

R

E
|
E

S

L

G

G

S

K

D

E

A

G

W

L

K

E

A

E

Y

Y

M

L

R

V

R

S

Q

K

T

H

N

I

T

L

I

T

N

N

Y

T

G

N

S

P

E

Q

L

L

active site: N166 (= N168), K189 (= K191), E264 (= E268), C298 (= C302), E399 (= E402), E476 (= E482)
binding nicotinamide-adenine-dinucleotide: P164 (≠ A166), K189 (= K191), E192 (= E194), G222 (= G226), G226 (= G230), G242 (= G246), G243 (≠ S247), T246 (≠ V250), H249 (= H253), I250 (≠ V254), C298 (= C302), E399 (= E402), F401 (= F404)

Query Sequence

>WP_035133104.1 NCBI__GCF_000769915.1:WP_035133104.1
MATLTDQFGIQEALSKLGIQEINEGTSTGSNSFANGEIIESYSPVDGTLIAKVKASTQED
YEAAVAKAQEAFKSWRLVPAPKRGEIVRQMGEELRKYKEPLGQLVSYEMGKSLQEGLGEV
QEMIDICDFAVGLSRQLYGLTMHSERPMHRMYEQYHPVGIVGIISAFNFPVAVWSWNAML
AWVCGDVCIWKPSEKTPLCGIACQNIIKTVLERNNIPEGVSCLINGRDNGDRMNTDKRLP
LVSFTGSTRVGRHVSKTVAERFGNTILELGGNNAIIVSEHADINMVLVGAVFGAVGTAGQ
RCTTTRRLIVHESVYDKTIEVLKSAYGQLKIGNPLDSNNHVGPLIDKDSVQNYLDAIEKA
KQEGGNVIVEGGVMQGEGYESGCYVKPCIIEAKNSFEIVQHETFAPILYVMKYNDLEEAI
AMQNDVPQGLSSSIFTSNMREMELFLSHAGSDCGIANVNIGTSGAEIGGAFGGEKETGGG
RESGSDAWKAYMRRQTNTINYGSELPLAQGIRFDL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory