SitesBLAST
Comparing WP_035133198.1 NCBI__GCF_000769915.1:WP_035133198.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2q6hA Crystal structure analysis of leut complexed with l-leucine, sodium, and clomipramine (see paper)
41% identity, 80% coverage: 4:485/602 of query aligns to 2:485/512 of 2q6hA
- binding 3-(3-chloro-5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R28 (= R30), Q32 (= Q34), R189 (≠ K180), F190 (≠ V181), I193 (= I184), F316 (= F311), F346 (= F343), L396 (≠ V394), D397 (= D396)
- binding leucine: A20 (= A22), G22 (= G24), G24 (= G26), Y106 (= Y108), F249 (= F247), T250 (= T248), S252 (= S250), F255 (≠ M253)
- binding sodium ion: G18 (= G20), A20 (= A22), V21 (= V23), V21 (= V23), G22 (= G24), N25 (= N27), T250 (= T248), N282 (= N280), A347 (= A344), T350 (= T347), S351 (= S348)
2qeiA Crystal structure analysis of leut complexed with l-alanine, sodium, and clomipramine (see paper)
41% identity, 80% coverage: 4:485/602 of query aligns to 1:484/511 of 2qeiA
- binding alanine: A19 (= A22), G21 (= G24), G23 (= G26), Y105 (= Y108), F248 (= F247), T249 (= T248), S251 (= S250)
- binding 3-(3-chloro-5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R27 (= R30), V30 (= V33), Q31 (= Q34), Y104 (= Y107), R180 (≠ K173), R188 (≠ K180), F189 (≠ V181), F315 (= F311), F345 (= F343), D396 (= D396), D399 (= D399)
- binding sodium ion: G17 (= G20), A19 (= A22), V20 (= V23), V20 (= V23), G21 (= G24), N24 (= N27), T249 (= T248), N281 (= N280), A346 (= A344), T349 (= T347), S350 (= S348)
2q72A Crystal structure analysis of leut complexed with l-leucine, sodium, and imipramine (see paper)
41% identity, 80% coverage: 4:485/602 of query aligns to 1:484/511 of 2q72A
- binding 3-(5h-dibenzo[b,f]azepin-5-yl)-n,n-dimethylpropan-1-amine: R27 (= R30), V30 (= V33), Q31 (= Q34), R188 (≠ K180), F189 (≠ V181), I192 (= I184), A314 (≠ G310), F315 (= F311), F345 (= F343), D396 (= D396)
- binding leucine: A19 (= A22), G21 (= G24), L22 (= L25), G23 (= G26), Y105 (= Y108), F248 (= F247), T249 (= T248), S251 (= S250), F254 (≠ M253)
- binding sodium ion: G17 (= G20), A19 (= A22), V20 (= V23), V20 (= V23), G21 (= G24), N24 (= N27), T249 (= T248), N281 (= N280), A346 (= A344), T349 (= T347), S350 (= S348)
3gwuA Leucine transporter leut in complex with sertraline (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/509 of 3gwuA
- binding leucine: A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253)
- binding (1S,4S)-4-(3,4-dichlorophenyl)-N-methyl-1,2,3,4-tetrahydronaphthalen-1-amine: L25 (= L29), R26 (= R30), Y104 (= Y108), F247 (= F247), A313 (≠ G310), D395 (= D396), D398 (= D399)
3f4jA Crystal structure of leut bound to glycine and sodium (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/509 of 3f4jA
- binding glycine: A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250)
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), G22 (= G26), N23 (= N27), T248 (= T248), N280 (= N280), A345 (= A344), G346 (= G345), T348 (= T347), S349 (= S348)
3f3dA Crystal structure of leut bound to l-methionine and sodium (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/509 of 3f3dA
- binding methionine: N17 (= N21), A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), S349 (= S348), I353 (≠ M352)
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T248 (= T248), N280 (= N280), A345 (= A344), T348 (= T347), S349 (= S348)
3f3cA Crystal structure of leut bound to 4-fluoro-l-phenylalanine and sodium (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/509 of 3f3cA
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T248 (= T248), N280 (= N280), A345 (= A344), T348 (= T347), S349 (= S348)
- binding 4-fluoro-l-phenylalanine: N17 (= N21), A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253), S349 (= S348), I353 (≠ M352)
2qb4A Crystal structure analysis of leut complexed with l-leucine, sodium and desipramine (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/510 of 2qb4A
- binding 3-(10,11-dihydro-5h-dibenzo[b,f]azepin-5-yl)-n-methylpropan-1-amine: R26 (= R30), V29 (= V33), Q30 (= Q34), I107 (= I111), R187 (≠ K180), F188 (≠ V181), I191 (= I184), A313 (≠ G310), F314 (= F311), F344 (= F343), D395 (= D396)
- binding leucine: A18 (= A22), G20 (= G24), L21 (= L25), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253)
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T248 (= T248), N280 (= N280), A345 (= A344), T348 (= T347), S349 (= S348)
3gwwA Leucine transporter leut in complex with s-fluoxetine (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:480/501 of 3gwwA
- binding leucine: A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F244 (= F247), T245 (= T248), S247 (= S250), F250 (≠ M253), I350 (≠ M352)
- binding (3S)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L25), G22 (= G26), R26 (= R30), Y104 (= Y108), A310 (≠ G310), F311 (= F311), D392 (= D396), D395 (= D399)
3mpnA F177r1 mutant of leut (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:483/505 of 3mpnA
- binding leucine: N17 (= N21), A18 (= A22), G20 (= G24), L21 (= L25), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253), S349 (= S348)
- binding S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate: C171 (≠ L165), A377 (≠ F375)
3uslA Crystal structure of leut bound to l-selenomethionine in space group c2 from lipid bicelles (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:482/502 of 3uslA
- binding selenomethionine: A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F246 (= F247), T247 (= T248), S249 (= S250), S348 (= S348), I352 (≠ M352)
- binding sodium ion: G16 (= G20), N17 (= N21), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T247 (= T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
- binding phosphocholine: N83 (≠ G87), R84 (= R88), F85 (≠ I89)
3usgA Crystal structure of leut bound to l-leucine in space group c2 from lipid bicelles (see paper)
41% identity, 80% coverage: 6:485/602 of query aligns to 2:482/502 of 3usgA
- binding leucine: A18 (= A22), G20 (= G24), G22 (= G26), F246 (= F247), T247 (= T248), S249 (= S250), F252 (≠ M253)
- binding sodium ion: G16 (= G20), N17 (= N21), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T247 (= T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
- binding phosphocholine: N83 (≠ G87), R84 (= R88)
3f3aA Crystal structure of leut bound to l-tryptophan and sodium (see paper)
40% identity, 80% coverage: 6:485/602 of query aligns to 2:482/504 of 3f3aA
- binding sodium ion: G16 (= G20), N17 (= N21), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T247 (= T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
- binding tryptophan: R7 (= R11), A18 (= A22), G20 (= G24), L21 (= L25), G22 (= G26), R26 (= R30), Y104 (= Y108), G242 (= G243), F246 (= F247), F246 (= F247), T247 (= T248), S249 (= S250), F252 (≠ M253), D265 (≠ K266), Q266 (≠ D267), D267 (= D268), F299 (≠ L300), N303 (vs. gap), A306 (≠ W304), I307 (≠ V305), A310 (≠ N308), N314 (≠ G312), S348 (= S348), I352 (≠ M352), D397 (= D399), G401 (= G403), T402 (= T404), G432 (= G434)
3uspA Crystal structure of leut in heptyl-beta-d-selenoglucoside (see paper)
41% identity, 76% coverage: 6:460/602 of query aligns to 2:459/503 of 3uspA
- binding heptyl 1-seleno-beta-D-glucopyranoside: K117 (≠ H121), L122 (≠ T126), E153 (vs. gap), I155 (vs. gap), K157 (vs. gap), P158 (vs. gap), F161 (vs. gap), Y163 (≠ V157), H385 (≠ T386), H385 (≠ T386), L386 (≠ V387), F389 (≠ Y390), F389 (≠ Y390), L394 (≠ F395), D395 (= D396), D398 (= D399), P439 (= P440), Y442 (≠ F443)
- binding leucine: A18 (= A22), G20 (= G24), G22 (= G26), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253)
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), G20 (= G24), N23 (= N27), T248 (= T248), N280 (= N280), A345 (= A344), T348 (= T347), S349 (= S348)
3gwvA Leucine transporter leut in complex with r-fluoxetine (see paper)
41% identity, 76% coverage: 6:460/602 of query aligns to 2:459/498 of 3gwvA
- binding leucine: A18 (= A22), G20 (= G24), G22 (= G26), Y104 (= Y108), F247 (= F247), T248 (= T248), S250 (= S250), F253 (≠ M253)
- binding (3R)-N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine: L21 (= L25), R26 (= R30), A313 (≠ G310), F314 (= F311), L394 (≠ F395), D395 (= D396), D398 (= D399)
6xwmA Mechanism of substrate release in neurotransmitter:sodium symporters: the structure of leut in an inward-facing occluded conformation (see paper)
41% identity, 79% coverage: 13:485/602 of query aligns to 1:477/498 of 6xwmA
- binding sodium ion: G8 (= G20), N9 (= N21), A10 (= A22), V11 (= V23), V11 (= V23), G12 (= G24), N15 (= N27), T242 (= T248), N274 (= N280), A339 (= A344), T342 (= T347), S343 (= S348)
- binding phenylalanine: N9 (= N21), A10 (= A22), G12 (= G24), G14 (= G26), Y96 (= Y108), F241 (= F247), T242 (= T248), S244 (= S250), F247 (≠ M253), S343 (= S348), I347 (≠ M352)
4mmeA Crystal structure of leubat (delta6 mutant) in complex with mazindol (see paper)
39% identity, 80% coverage: 6:485/602 of query aligns to 2:482/504 of 4mmeA
- binding (5R)-5-(4-chlorophenyl)-2,5-dihydro-3H-imidazo[2,1-a]isoindol-5-ol: Y17 (≠ N21), A18 (= A22), D20 (≠ G24), V100 (= V104), A101 (= A105), Y104 (= Y108), F246 (= F247), G249 (≠ S250), F252 (≠ M253), S348 (= S348), G352 (≠ M352)
- binding sodium ion: G16 (= G20), Y17 (≠ N21), A18 (= A22), V19 (= V23), V19 (= V23), D20 (≠ G24), D20 (≠ G24), N23 (= N27), S247 (≠ T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
4mm6A Crystal structure of leubat (delta13 mutant) in complex with (s)- duloxetine (see paper)
39% identity, 80% coverage: 6:485/602 of query aligns to 2:482/504 of 4mm6A
- binding (3S)-N-methyl-3-(naphthalen-1-yloxy)-3-(thiophen-2-yl)propan-1-amine: Y17 (≠ N21), A18 (= A22), D20 (≠ G24), P97 (≠ N101), V100 (= V104), A101 (= A105), Y104 (= Y108), F246 (= F247), S247 (≠ T248), F252 (≠ M253), S349 (= S349), G352 (≠ M352), T401 (≠ G403)
- binding sodium ion: G16 (= G20), A18 (= A22), V19 (= V23), V19 (= V23), N23 (= N27), S247 (≠ T248), N279 (= N280), A344 (= A344), G345 (= G345), T347 (= T347), S348 (= S348)
4mm9A Crystal structure of leubat (delta13 mutant) in complex with fluvoxamine (see paper)
40% identity, 76% coverage: 6:460/602 of query aligns to 2:458/499 of 4mm9A
- binding Fluvoxamine: Y17 (≠ N21), A18 (= A22), D20 (≠ G24), V100 (= V104), A101 (= A105), Y104 (= Y108), F246 (= F247), S247 (≠ T248), F252 (≠ M253), G352 (≠ M352)
- binding sodium ion: G16 (= G20), Y17 (≠ N21), A18 (= A22), V19 (= V23), V19 (= V23), D20 (≠ G24), N23 (= N27), S247 (≠ T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
4mm7A Crystal structure of leubat (delta13 mutant) in complex with desvenlafaxine (see paper)
40% identity, 76% coverage: 6:460/602 of query aligns to 2:458/499 of 4mm7A
- binding 4-[(1R)-2-(dimethylamino)-1-(1-hydroxycyclohexyl)ethyl]phenol: Y17 (≠ N21), V100 (= V104), Y104 (= Y108), F246 (= F247), F252 (≠ M253), S348 (= S348)
- binding sodium ion: G16 (= G20), Y17 (≠ N21), A18 (= A22), V19 (= V23), V19 (= V23), D20 (≠ G24), N23 (= N27), S247 (≠ T248), N279 (= N280), A344 (= A344), T347 (= T347), S348 (= S348)
Query Sequence
>WP_035133198.1 NCBI__GCF_000769915.1:WP_035133198.1
MAAKAESWGSRVGLILAMAGNAVGLGNFLRFPVQAVQNGGGAFIIPYLVCFVVMGIPLLF
IEWSTGRFGGRYGNHSTPYILDTMAKGRIWKYIGVFGIFTNIAVASYYCYIESWTMSYVY
HTIKGTFVGMDQAQVAGFFDTYTTIAKSTTGIPYEAVVFYIACLLLNTYILSKGLGGIEK
VAKIGMPLLILFGAVLAFKGLTLGTDGASDMFPNANAWDGLNFLWTPQFDSLWSAKVWLA
AAGQIFFTLSVGMGTVHCYAAYIKSKDDIALNAVSAGFMNEFVEVVLGSSIVIPIAAGYL
GLDWVLQNAGFGMAFQTMPYLFQQWGHVLAVFAGVFWFGLLFFAGITSSLAMGTPWIGFM
RDEFGWKNNKGAWSFGLIALIMGLPTVIFYKEGVFDEYDYWAGTVSLVVFAFLETVLFAW
IFGMKRGWEEINLGADIKVPVFFKYVILVVTPCLLGWVLLASTPDIANKVMNNDTHNKEW
FADAYYAEDYGSAEFGVVDSVAPNFVKIAFENEKQVFDKTQNTVNTIKYTDYKTYAFKGE
QHTVVSLGDKIHPKDKIAEGKFTNNVMYKTIGRLLLITLFIFISVVVYRAYLKRVKEGRA
TA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory