SitesBLAST

YI 11:12 (≠ FI 11:12) binding NAD(+)
DNLCNS 31:36 (≠ DDLSNS 31:36) binding NAD(+)
DI 58:59 (≠ DL 58:59) binding NAD(+)
FAGLK 80:84 (≠ FAASK 81:85) binding NAD(+)
N99 (= N100) binding NAD(+)
S124 (= S125) binding NAD(+); mutation to A: No major structural changes. Catalytic efficiency is very low and affinity binding is 21% of the wild-type enzyme.; mutation to T: No major structural changes. Catalytic efficiency is about 30% of that of the wild-type enzyme, and affinity binding is similar to that of the native enzyme.
Y149 (= Y150) active site, Proton acceptor; binding NAD(+); mutation to F: No major structural changes. Catalytic efficiency is very low and affinity binding is 12% of the wild-type enzyme.
K153 (= K154) binding NAD(+); mutation to A: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.; mutation to M: Decreases the catalytic activity. Not reduced by sugars in the presence or absence of UMP. It contains very little NADH.
F178 (= F178) binding NAD(+)
Y299 (≠ A302) mutation to C: Loss of epimerase activity with UDP-Gal by almost 5-fold, but it results in a gain of epimerase activity with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) by 230-fold with minimal changes in its three-dimensional structure.

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
47% identity, 99% coverage: 1:334/338 of query aligns to 1:331/338 of 1udaA

query
sites
1udaA

M

M

K

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

D

D

V

V

V

I

I

L

D
|
D

D
x
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

L

R

D

S

V

V

L

L

N

P

G

V

I

I

E

E

S

R

I

L

T

G

G

G

K

K

K

H

P

P

E

T

F

F

E

V

K

E

I

G

D
|
D

L
x
I

R

R

E

N

K

E

Q

A

A

L

V

M

K

T

D

E

F

I

F

L

V

H

K

D

Y

H

P

A

D

-

V

I

T

D

G

T

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

I

V

G

N

S

G

L

T

V

L

Y

R

L

L

L

I

Q

S

E

A

L

M

Q

R

K

A

Q

N

E

V

F

K

N

N

F

F

I

I

F

F

S
|
S

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

E

P

K

K

M

I

P

P

I

Y

T

V

E

E

G

S

A

F

P

P

I

T

Q

G

G

T

A

P

L

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

I

M

G

V

E

E

E

Q

I

I

I

L

K

T

D

D

V

L

A

Q

K

K

V

A

S

Q

G

P

I

D

N

W

S

S

V

I

-

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

D

I
x
M

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

D

Q

S

L

L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

L

V

A

A

L

M

Q

E

R

K

L

L

I

A

D

N

K

K

K

P

N

G

A

-

E

-

K

-

V

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

K

V

G

G

N

N

S

S

V

V

L

L

E

D

V

V

I

V

N

N

A

A

F

F

E

S

R

K

V

A

S

C

G

G

Q

K

K

P

L

V

N

N

Y

Y

K

H

I

F

V

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

V

L

A

P

E

A

A
x
Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

N

R

V

E

L

L

G

N

W

W

K

R

A

V

E

T

L

R

S

T

L

L

D

D

D

E

A

M

L

A

A

Q

S

D

A

T

W

W

K

H

W

W

E

Q

Q

S

K

R

active site: S124 (= S125), A125 (≠ C126), T126 (= T127), Y149 (= Y150), K153 (= K154), M189 (≠ I189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (≠ L59), F80 (= F81), A81 (= A82), G82 (≠ A83), K84 (= K85), S122 (= S123), Y149 (= Y150), K153 (= K154), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (= T127), N179 (= N179), N199 (= N199), L200 (= L200), A216 (≠ S216), I217 (≠ V217), F218 (= F218), R231 (= R231), Y233 (= Y233), R292 (= R295), D295 (= D298), Y299 (≠ A302)

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
47% identity, 99% coverage: 1:334/338 of query aligns to 1:331/338 of 1naiA

query
sites
1naiA

M

M

K

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

D

D

V

V

V

I

I

L

D
|
D

D
x
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

L

R

D

S

V

V

L

L

N

P

G

V

I

I

E

E

S

R

I

L

T

G

G

G

K

K

K

H

P

P

E

T

F

F

E

V

K

E

I

G

D
|
D

L
x
I

R

R

E

N

K

E

Q

A

A

L

V

M

K

T

D

E

F

I

F

L

V

H

K

D

Y

H

P

A

D

-

V

I

T

D

G

T

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

I

V

G

N

S

G

L

T

V

L

Y

R

L

L

L

I

Q

S

E

A

L

M

Q

R

K

A

Q

N

E

V

F

K

N

N

F

F

I

I

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

E

P

K

K

M

I

P

P

I

Y

T

V

E

E

G

S

A

F

P

P

I

T

Q

G

G

T

A

P

L

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

I

M

G

V

E

E

E

Q

I

I

I

L

K

T

D

D

V

L

A

Q

K

K

V

A

S

Q

G

P

I

D

N

W

S

S

V

I

-

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

D

I
x
M

G

G

E
|
E

L

D

P
|
P

L

Q

G

G

V

I

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

D

Q

S

L
|
L

S
x
A

V

I

F

F

G

G

N

N

D

D

Y

Y

P

P

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

L

V

A

A

L

M

Q

E

R

K

L

L

I

A

D

N

K

K

K

P

N

G

A

-

E

-

K

-

V

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

K

V

G

G

N

N

S

S

V

V

L

L

E

D

V

V

I

V

N

N

A

A

F

F

E

S

R

K

V

A

S

C

G

G

Q

K

K

P

L

V

N

N

Y

Y

K

H

I

F

V

A

D

P

R

R

R
|
R

A

E

G

G

D

D

V

L

A

P

E

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

N

R

V

E

L

L

G

N

W

W

K

R

A

V

E

T

L

R

S

T

L

L

D

D

D

E

A

M

L

A

A

Q

S

D

A

T

W

W

K

H

W

W

E

Q

Q

S

K

R

active site: S124 (= S125), A125 (≠ C126), T126 (= T127), Y149 (= Y150), K153 (= K154), M189 (≠ I189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (≠ L59), F80 (= F81), A81 (= A82), G82 (≠ A83), K84 (= K85), Y149 (= Y150), K153 (= K154), Y177 (= Y177), P180 (= P180)
binding 1,3-propandiol: N35 (= N35), K84 (= K85), E191 (= E191), P193 (= P193)
binding uridine-5'-diphosphate: N179 (= N179), N199 (= N199), L200 (= L200), L215 (= L215), A216 (≠ S216), R231 (= R231), Y233 (= Y233), R292 (= R295)

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
47% identity, 99% coverage: 1:334/338 of query aligns to 1:331/338 of 1lrjA

query
sites
1lrjA

M

M

K

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

D

D

V

V

V

I

I

L

D
|
D

D
x
N

L
|
L

S
x
C

N
|
N

S
|
S

S

K

L

R

D

S

V

V

L

L

N

P

G

V

I

I

E

E

S

R

I

L

T

G

G

G

K

K

K

H

P

P

E

T

F

F

E

V

K

E

I

G

D
|
D

L
x
I

R

R

E

N

K

E

Q

A

A

L

V

M

K

T

D

E

F

I

F

L

V

H

K

D

Y

H

P

A

D

-

V

I

T

D

G

T

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V
|
V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

I

V

G

N

S

G

L

T

V

L

Y

R

L

L

L

I

Q

S

E

A

L

M

Q

R

K

A

Q

N

E

V

F

K

N

N

F

F

I

I

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

N

E

P

K

K

M

I

P

P

I

Y

T

V

E

E

G

S

A

F

P

P

I

T

Q

G

G

T

A

P

L

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

I

M

G

V

E

E

E

Q

I

I

I

L

K

T

D

D

V

L

A

Q

K

K

V

A

S

Q

G

P

I

D

N

W

S

S

V

I

-

A

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

D

I
x
M

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L

N

N

N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

D

Q

S

L
|
L

S
x
A

V

I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

L

V

A

A

L

M

Q

E

R

K

L

L

I

A

D

N

K

K

K

P

N

G

A

-

E

-

K

-

V

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

K

V

G

G

N

N

S

S

V
|
V

L

L

E

D

V

V

I

V

N

N

A

A

F

F

E

S

R

K

V

A

S

C

G

G

Q

K

K

P

L

V

N

N

Y

Y

K

H

I

F

V

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

V

L

A

P

E

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

N

R

V

E

L

L

G

N

W

W

K

R

A

V

E

T

L

R

S

T

L

L

D

D

D

E

A

M

L

A

A

Q

S

D

A

T

W

W

K

H

W

W

E

Q

Q

S

K

R

active site: S124 (= S125), A125 (≠ C126), T126 (= T127), Y149 (= Y150), K153 (= K154), M189 (≠ I189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), L33 (= L33), C34 (≠ S34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (≠ L59), F80 (= F81), A81 (= A82), G82 (≠ A83), K84 (= K85), Y149 (= Y150), K153 (= K154), Y177 (= Y177), P180 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: V86 (= V87), S124 (= S125), F178 (= F178), N179 (= N179), L200 (= L200), L215 (= L215), A216 (≠ S216), F218 (= F218), R231 (= R231), Y233 (= Y233), V269 (= V272), R292 (= R295), D295 (= D298)

1kvrA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
47% identity, 99% coverage: 1:334/338 of query aligns to 1:331/338 of 1kvrA

query
sites
1kvrA

M

M

K

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

D

D

V

V

V

I

I

L

D
|
D

D
x
N

L

L

S
x
C

N
|
N

S
|
S

S

K

L

R

D

S

V

V

L

L

N

P

G

V

I

I

E

E

S

R

I

L

T

G

G

G

K

K

K

H

P

P

E

T

F

F

E

V

K

E

I

G

D
|
D

L
x
I

R

R

E

N

K

E

Q

A

A

L

V

M

K

T

D

E

F

I

F

L

V

H

K

D

Y

H

P

A

D

-

V

I

T

D

G

T

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

I

V

G

N

S

G

L

T

V

L

Y

R

L

L

L

I

Q

S

E

A

L

M

Q

R

K

A

Q

N

E

V

F

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

Q

E

P

K

K

M

I

P

P

I

Y

T

V

E

E

G

S

A

F

P

P

I

T

Q

G

G

T

A

P

L

Q

S

S

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

L

I

M

G

V

E

E

E

Q

I

I

I

L

K

T

D

D

V

L

A

Q

K

K

V

A

S

Q

G

P

I

D

N

W

S

S

V

I

-

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

D

I
x
M

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L
x
N

N
|
N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

D

Q

S

L

L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

L

V

A

A

L

M

Q

E

R

K

L

L

I

A

D

N

K

K

K

P

N

G

A

-

E

-

K

-

V

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

K

V

G

G

N

N

S

S

V
|
V

L

L

E

D

V

V

I

V

N

N

A

A

F

F

E

S

R

K

V

A

S

C

G

G

Q

K

K

P

L

V

N

N

Y

Y

K

H

I

F

V

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

V

L

A

P

E

A

A

Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

N

R

V

E

L

L

G

N

W

W

K

R

A

V

E

T

L

R

S

T

L

L

D

D

D

E

A

M

L

A

A

Q

S

D

A

T

W

W

K

H

W

W

E

Q

Q

S

K

R

active site: A124 (≠ S125), A125 (≠ C126), T126 (= T127), Y149 (= Y150), K153 (= K154), M189 (≠ I189)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), C34 (≠ S34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (≠ L59), F80 (= F81), A81 (= A82), G82 (≠ A83), K84 (= K85), S122 (= S123), S123 (= S124), Y149 (= Y150), K153 (= K154), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate: N179 (= N179), N198 (≠ L198), N199 (= N199), L200 (= L200), A216 (≠ S216), I217 (≠ V217), F218 (= F218), R231 (= R231), Y233 (= Y233), V269 (= V272), R292 (= R295), D295 (= D298)

1a9yA Udp-galactose 4-epimerase mutant s124a/y149f complexed with udp- glucose (see paper)
46% identity, 99% coverage: 1:334/338 of query aligns to 1:331/338 of 1a9yA

query
sites
1a9yA

M

M

K

R

I

V

L

L

V

V

T

T

G
|
G

G

G

L

S

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

C

V

V

E

Q

L

L

Q

L

N

Q

E

N

G

G

F

H

D

D

V

V

V

I

I

L

D
|
D

D
x
N

L

L

S
x
C

N
|
N

S
|
S

S

K

L

R

D

S

V

V

L

L

N

P

G

V

I

I

E

E

S

R

I

L

T

G

G

G

K

K

K

H

P

P

E

T

F

F

E

V

K

E

I

G

D
|
D

L
x
I

R

R

E

N

K

E

Q

A

A

L

V

M

K

T

D

E

F

I

F

L

V

H

K

D

Y

H

P

A

D

-

V

I

T

D

G

T

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

E

Y

Y

E

D

N

N

I

V

G

N

S

G

L

T

V

L

Y

R

L

L

L

I

Q

S

E

A

L

M

Q

R

K

A

Q

N

E

V

F

K

N

N

F

F

I

I

F

F

S
|
S

S
x
A

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

Q

E

P

K

K

M

I

P

P

I

Y

T

V

E

E

G

S

A

F

P

P

I

T

Q

G

G

T

A

P

L

Q

S

S

P

P

Y
x
F

G

G

N

K

T

S

K
|
K

Q

L

I

M

G

V

E

E

E

Q

I

I

I

L

K

T

D

D

V

L

A

Q

K

K

V

A

S

Q

G

P

I

D

N

W

S

S

V

I

-

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

D

I

M

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

D

Q

S

L

L

S
x
A

V
x
I

F
|
F

G

G

N

N

D

D

Y

Y

P

P

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

V

L

V

A

A

L

M

Q

E

R

K

L

L

I

A

D

N

K

K

K

P

N

G

A

-

E

-

K

-

V

V

E

H

V

I

F

Y

N

N

L

L

G

G

T

A

G

G

K

V

G

G

N

N

S

S

V

V

L

L

E

D

V

V

I

V

N

N

A

A

F

F

E

S

R

K

V

A

S

C

G

G

Q

K

K

P

L

V

N

N

Y

Y

K

H

I

F

V

A

D

P

R

R

R
|
R

A

E

G

G

D
|
D

V

L

A

P

E

A

A
x
Y

Y

W

A

A

D

D

T

A

A

S

K

K

A

A

N

D

N

R

V

E

L

L

G

N

W

W

K

R

A

V

E

T

L

R

S

T

L

L

D

D

D

E

A

M

L

A

A

Q

S

D

A

T

W

W

K

H

W

W

E

Q

Q

S

K

R

active site: A124 (≠ S125), A125 (≠ C126), T126 (= T127), F149 (≠ Y150), K153 (= K154)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (= I12), D31 (= D31), N32 (≠ D32), C34 (≠ S34), N35 (= N35), S36 (= S36), D58 (= D58), I59 (≠ L59), F80 (= F81), A81 (= A82), G82 (≠ A83), K84 (= K85), S122 (= S123), S123 (= S124), F149 (≠ Y150), K153 (= K154), Y177 (= Y177), P180 (= P180)
binding uridine-5'-diphosphate-glucose: A125 (≠ C126), T126 (= T127), N179 (= N179), N199 (= N199), L200 (= L200), A216 (≠ S216), I217 (≠ V217), F218 (= F218), R231 (= R231), Y233 (= Y233), R292 (= R295), D295 (= D298), Y299 (≠ A302)

3enkA 1.9a crystal structure of udp-glucose 4-epimerase from burkholderia pseudomallei
51% identity, 98% coverage: 3:333/338 of query aligns to 7:333/340 of 3enkA

query
sites
3enkA

I

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

A

V

V

E

E

L

L

Q

L

N

A

E

H

G

G

F

Y

D

D

V

V

V

I

I

A

D
|
D

D
x
N

L

L

S
x
V

N
|
N

S
|
S

S

K

L

R

D

E

V

A

L

I

N

A

G

R

I

I

E

E

S

K

I

I

T

T

G

G

K

K

K

T

P

P

E

A

F

F

E

H

K

E

I

T

D
|
D

L
x
V

R

S

E

D

K

E

Q

R

A

A

V

L

K

A

D

R

F

I

F

F

V

D

K

A

Y

H

P

P

D

-

V

I

T

T

G

A

I

A

I

I

H

H

F
|
F

A
|
A

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

A

N

K

P

P

L

I

L

E

Y

Y

E

R

N
|
N

N

N

I

L

G

D

S

S

L

L

V

L

Y

S

L

L

Q

R

E

V

L

M

Q

R

K

E

K

R

Q

A

E

V

F

K

N

R

F

I

I

V

F

F

S
|
S

C

A

T
|
T

V

V

Y

Y

G

G

Q

V

A

P

E

E

K

R

M

S

P

P

I

I

T

D

E

E

G

T

A

F

P

P

I

L

Q

S

G

-

A

A

L

T

S

N

P

P

Y
|
Y

G

G

N

Q

T

T

K
|
K

Q

L

I

M

G

A

E

E

E

Q

I

I

I

L

K

R

D

D

V

V

-

E

A

A

K

A

V

D

S

P

G

S

I

W

N

R

S

V

V

A

L

T

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

E

S

S

V

G

N

L

I

I

G

G

E

E

L

D

P

P

L

A

G

G

V

I

P

P

L
x
N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

A

Q

Q

T

V

A

A

I

V

G

G

I

K

R

L

E

E

Q

K

L

L

S
x
R

V
|
V

F
|
F

G

G

N

S

D

D

Y

Y

P

P

T

T

T

P

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

R

A

G

H

H

V

I

L

A

A

A

L

L

Q

D

R

A

L

L

I

-

D

E

K

R

N

D

A

A

E

S

K

L

V

-

E

-

V

T

F

V

N

N

L

L

G

G

T

T

G

G

K

R

G

G

N

Y

S

S

V
|
V

L

L

E

E

V

V

I

V

N

R

A

A

F

F

E

E

R

K

V

A

S

S

G

G

Q

R

K

A

L

V

N

P

Y

Y

K

E

I

L

V

V

D

A

R

R

R
|
R

A

P

G

G

D
|
D

V

V

A

A

E

E

A

C

Y

Y

A

A

D

N

T

P

A

A

K

A

A

A

N

A

N

E

V

T

L

I

G

G

W

W

K

K

A

A

E

E

L

R

S

D

L

L

D

E

D

R

A

M

L

C

A

A

S

D

A

H

W

W

K

R

W

W

E

Q

Q

E

active site: S127 (= S124), S128 (= S125), T130 (= T127), Y152 (= Y150), K156 (= K154)
binding nicotinamide-adenine-dinucleotide: G11 (= G7), G14 (= G10), Y15 (≠ F11), I16 (= I12), D35 (= D31), N36 (≠ D32), V38 (≠ S34), N39 (= N35), S40 (= S36), D62 (= D58), V63 (≠ L59), F84 (= F81), A85 (= A82), A86 (= A83), K88 (= K85), N103 (= N100), S126 (= S123), S128 (= S125), Y152 (= Y150), K156 (= K154), Y180 (= Y177), P183 (= P180)
binding uridine-5'-diphosphate-glucose: T130 (= T127), N182 (= N179), N201 (≠ L198), N202 (= N199), L203 (= L200), R219 (≠ S216), V220 (= V217), F221 (= F218), R234 (= R231), Y236 (= Y233), V272 (= V272), R295 (= R295), D298 (= D298)

1ek6A Structure of human udp-galactose 4-epimerase complexed with nadh and udp-glucose (see paper)
48% identity, 99% coverage: 2:334/338 of query aligns to 4:339/346 of 1ek6A

query
sites
1ek6A

K

K

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

D

L

V

P

V

V

I

I

D
|
D

D
x
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

L

P

D

E

V

S

L

L

N

R

G

R

I

V

E

Q

S

E

I

L

T

T

G

G

K

R

K

S

P

V

E

E

F

F

E

E

K

E

I

M

D
|
D

L
x
I

R

L

E

D

K

Q

Q

G

A

A

V

L

K

Q

D

R

F

L

F

F

V

K

K

K

Y

Y

P

-

D

S

V

F

T

M

G

A

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

D

Y

Y

E

R

N

V

N

N

I

L

G

T

S

G

L

T

V

I

Y

Q

L

L

Q

E

E

I

L

M

Q

K

K

A

K

H

Q

G

E

V

F

K

N

N

F

L

I

V

F

F

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

N

A

P

E

Q

K

Y

M

L

P

P

I

L

T

D

E

E

G

A

A

H

P

P

I

T

Q

G

G

G

A

C

L

T

S

N

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

F

I

F

G

I

E

E

I

M

I

I

K

R

D

D

V

L

A

C

K

Q

V

A

S

D

G

K

I

T

-

W

N

N

S

A

V

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

V

G

N

C

I

I

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

S

Q

Q

T

V

A

A

I

I

G

G

I

R

R

R

E

E

Q

A

L

L

S
x
N

V
|
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

-

D

-

K

-

K

K

N

E

A

Q

E

C

K

G

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C

E

R

V

I

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Y

N

N

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L

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G

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T

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G

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|
V

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L

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Q

A

A

F

M

E

E

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K

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A

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S

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G

Q

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K

L

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N

P

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Y

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A

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R

A

E

G

G

D
|
D

V

V

A

A

E

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

N

E

V

E

L

L

G

G

W

W

K

T

A

A

E

A

L

L

S

G

L

L

D

D

D

R

A

M

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C

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W

K

R

W

W

E

Q

Q

K

K

Q

active site: S132 (= S125), A133 (≠ C126), T134 (= T127), Y157 (= Y150), K161 (= K154)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ D32), H36 (≠ S34), N37 (= N35), D66 (= D58), I67 (≠ L59), F88 (= F81), A89 (= A82), G90 (≠ A83), K92 (= K85), S130 (= S123), S131 (= S124), S132 (= S125), Y157 (= Y150), K161 (= K154), Y185 (= Y177), P188 (= P180)
binding uridine-5'-diphosphate-glucose: S132 (= S125), Y157 (= Y150), F186 (= F178), N187 (= N179), N207 (= N199), L208 (= L200), N224 (≠ S216), V225 (= V217), F226 (= F218), R239 (= R231), Y241 (= Y233), V277 (= V272), R300 (= R295), D303 (= D298)

Q14376 UDP-glucose 4-epimerase; Galactowaldenase; UDP-N-acetylgalactosamine 4-epimerase; UDP-GalNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-galactose 4-epimerase; EC 5.1.3.2; EC 5.1.3.7 from Homo sapiens (Human) (see 18 papers)
48% identity, 99% coverage: 2:334/338 of query aligns to 4:339/348 of Q14376

query
sites
Q14376

K

K

I

V

L

L

V

V

T

T

G

G

L

A

G
|
G

F
x
Y

I
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I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

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E

E

A

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G

F

Y

D

L

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V

V

I

I

D
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D

D
x
N

L
x
F

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x
H

N
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N

-

A

-

F

-

R

-

G

S

S

S

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L

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D

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N

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x
R

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F

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x
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K

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K

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L

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x
D

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Y

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N

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G

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L

Q

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E

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L

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K

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N

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x
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S

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x
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T

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Y

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x
T

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D

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N

N

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x
A

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L

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Y

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F

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N

P

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A

A

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A

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C

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E

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x
N

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L

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N

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x
K

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-

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-

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E

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D

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A

A
x
C

Y

Y

A

A

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x
L

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K

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Q

GYI 12:14 (≠ GFI 10:12) binding NAD(+)
DNFHN 33:37 (≠ DDLSN 31:35) binding NAD(+)
N34 (≠ D32) to S: in GALAC3; peripheral; nearly normal activity towards UDP-galactose; dbSNP:rs121908046
R51 (≠ G43) to W: in THC13; likely pathogenic; decreased UDP-galactose epimerization activity; decreased UDP-N-acetylglucosamine epimerase activity; reduced NAD+ binding; decreased thermal stability
DI 66:67 (≠ DL 58:59) binding NAD(+)
S81 (≠ D74) mutation to R: 5-fold decreased catalytic efficiency for UDP-galactose epimerization.
F88 (= F81) binding NAD(+)
G90 (≠ A83) to E: in GALAC3; 800-fold decrease in UDP-galactose epimerization activity; dbSNP:rs28940882
K92 (= K85) binding NAD(+)
V94 (= V87) to M: in GALAC3; generalized; 30-fold decrease in UDP-galactose epimerization activity; 2-fold decrease in affinity for UDP-galactose; 24% of normal activity with respect to UDP-N-acetylgalactosamine; dbSNP:rs121908047
D103 (≠ L96) to G: in GALAC3; uncertain significance; 7-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs28940883
V128 (≠ I121) to M: in THC13; likely pathogenic
S132 (= S125) mutation to A: Loss of activity.
SAT 132:134 (≠ SCT 125:127) binding substrate
T150 (≠ I143) to M: in THC13; likely pathogenic; 3-fold decreased catalytic efficiency for UDP-galactose epimerization
Y157 (= Y150) active site, Proton acceptor; mutation to F: Loss of activity.
K161 (= K154) binding NAD(+)
A180 (≠ S172) to V: in dbSNP:rs3204468
L183 (= L175) to P: in GALAC3; peripheral; 3-fold decrease in UDP-galactose epimerization activity; dbSNP:rs121908045
Y185 (= Y177) binding NAD(+)
YFN 185:187 (= YFN 177:179) binding substrate
NNL 206:208 (≠ LNL 198:200) binding substrate
L223 (= L215) to P: in THC13; likely pathogenic
NVF 224:226 (≠ SVF 216:218) binding substrate
G237 (≠ C229) to D: in THC13; uncertain significance
R239 (= R231) binding substrate
K257 (≠ R249) to R: in GALAC3; 7-fold decrease in UDP-galactose epimerization activity; does not affect affinity for UDP-galactose; dbSNP:rs28940884
REGD 300:303 (≠ RAGD 295:298) binding substrate
C307 (≠ A302) mutation to Y: No effect on activity towards UDP-galactose. Loss of activity towards UDP-N-acetylgalactosamine.
L313 (≠ K308) to M: in GALAC3; 6-fold decrease in UDP-galactose epimerization activity; very mild decrease in activity towards UDP-N-acetylgalactosamine; dbSNP:rs3180383
G319 (= G314) to E: in GALAC3; nearly normal activity towards UDP-galactose; mild impairment under conditions of substrate limitation; dbSNP:rs28940885
R335 (≠ K330) to H: in GALAC3; 2-fold decrease in UDP-galactose epimerization activity; dbSNP:rs368637540

1hzjA Human udp-galactose 4-epimerase: accommodation of udp-n- acetylglucosamine within the active site (see paper)
48% identity, 99% coverage: 2:334/338 of query aligns to 3:338/345 of 1hzjA

query
sites
1hzjA

K

K

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
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G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

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N

E

E

A

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G

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Y

D

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V

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I

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D

D
x
N

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x
H

N
|
N

-

A

-

F

-

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-

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S

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S

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Q

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A

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K

K

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Y

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F

A
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A

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x
G

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K

A

A

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V

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G

E

E

S

S

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V

E

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N

K

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P

L

L

L

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Y

Y

E

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N

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N

N

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G

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L

L

Q

E

E

I

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K

K

A

K

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K

N

N

F

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S
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S

S

S

S
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S

C
x
A

T
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T

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V

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Y

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G

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A

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E

E

G

A

A

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G

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Y

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K

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D

V

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A

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K

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W

N

N

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V

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L

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Y
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Y

F

F

N
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N

P
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P

V

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G

G

A

A

H

H

P

A

S

S

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G

N

C

I

I

G

G

E

E

L

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G

G

V

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P

L

N

N
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N

L
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L

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Y

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Q

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A

A

I

I

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G

I

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R

R

E

E

Q

A

L

L

S
x
N

V
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V

F
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F

G

G

N

N

D

D

Y

Y

P

D

T

T

T

E

D

D

G

G

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G

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R
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R

D

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Y
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Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

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L

A

A

A

L

L

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R

K

L

L

I

-

D

-

K

-

K

K

N

E

A

Q

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C

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R

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I

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Y

N

N

L

L

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G

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V

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V

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A

A

F

M

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E

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K

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A

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S

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G

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K

K

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Y

K

K

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V

V

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R

R

R
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R

A

E

G

G

D
|
D

V

V

A

A

E

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

N

E

V

E

L

L

G

G

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W

K

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A

A

E

A

L

L

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G

L

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D

D

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A

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W

K

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W

W

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Q

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Q

active site: S131 (= S125), A132 (≠ C126), T133 (= T127), Y156 (= Y150), K160 (= K154)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (= N35), D65 (= D58), I66 (≠ L59), F87 (= F81), A88 (= A82), G89 (≠ A83), K91 (= K85), S129 (= S123), S131 (= S125), Y156 (= Y150), K160 (= K154), Y184 (= Y177), P187 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ S216), V224 (= V217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V272), R299 (= R295), D302 (= D298)

1i3lA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
48% identity, 99% coverage: 2:334/338 of query aligns to 3:338/347 of 1i3lA

query
sites
1i3lA

K

K

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

D

L

V

P

V

V

I

I

D
|
D

D
x
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

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S

S

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L

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D

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V

S

L

L

N

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T

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G

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Q

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A

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F

A
|
A

A
x
G

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K

A

A

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G

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E

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S

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V

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K

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P

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L

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Y

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N

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L

L

Q

E

E

I

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K

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K

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N

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F

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S

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A

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Y

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E

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A

A

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Y

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D

V

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K

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N

N

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Y

F

F

N
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N

P
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P

V

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G

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A

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A

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C

I

I

G

G

E

E

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G

G

V

I

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L

N

N
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N

L
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L

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Q

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A

A

I

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G

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x
N

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F

G

G

N

N

D

D

Y

Y

P

D

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

-

D

-

K

-

K

K

N

E

A

Q

E

C

K

G

V

C

E

R

V

I

F

Y

N

N

L

L

G

G

T

T

G

G

K

T

G

G

N

Y

S

S

V
|
V

L

L

E

Q

V

M

I

V

N

Q

A

A

F

M

E

E

R

K

V

A

S

S

G

G

Q

K

K

K

L

I

N

P

Y

Y

K

K

I

V

V

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

V

V

A

A

E

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

N

E

V

E

L

L

G

G

W

W

K

T

A

A

E

A

L

L

S

G

L

L

D

D

D

R

A

M

L

C

A

E

S

D

A

L

W

W

K

R

W

W

E

Q

Q

K

K

Q

active site: S131 (= S125), A132 (≠ C126), T133 (= T127), Y156 (= Y150), K160 (= K154)
binding galactose-uridine-5'-diphosphate: N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ S216), V224 (= V217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V272), R299 (= R295), D302 (= D298)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (= N35), D65 (= D58), I66 (≠ L59), F87 (= F81), A88 (= A82), G89 (≠ A83), K91 (= K85), S129 (= S123), S130 (= S124), S131 (= S125), Y156 (= Y150), K160 (= K154), Y184 (= Y177), P187 (= P180)

1i3kA Molecular basis for severe epimerase-deficiency galactosemia: x-ray structure of the human v94m-substituted udp-galactose 4-epimerase (see paper)
48% identity, 99% coverage: 2:334/338 of query aligns to 3:338/347 of 1i3kA

query
sites
1i3kA

K

K

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

E

L

L

Q

L

N

E

E

A

G

G

F

Y

D

L

V

P

V

V

I

I

D
|
D

D
x
N

L

F

S
x
H

N
|
N

-

A

-

F

-

R

-

G

S

S

S

L

L

P

D

E

V

S

L

L

N

R

G

R

I

V

E

Q

S

E

I

L

T

T

G

G

K

R

K

S

P

V

E

E

F

F

E

E

K

E

I

M

D
|
D

L
x
I

R

L

E

D

K

Q

Q

G

A

A

V

L

K

Q

D

R

F

L

F

F

V

K

K

K

Y

Y

P

-

D

S

V

F

T

M

G

A

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

M

G

G

E

E

S

S

V

V

E

Q

N

K

P

P

L

L

L

D

Y

Y

E

R

N

V

N

N

I

L

G

T

S

G

L

T

V

I

Y

Q

L

L

Q

E

E

I

L

M

Q

K

K

A

K

H

Q

G

E

V

F

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

N

A

P

E

Q

K

Y

M

L

P

P

I

L

T

D

E

E

G

A

A

H

P

P

I

T

Q

G

G

G

A

C

L

T

S

N

P

P

Y
|
Y

G

G

N

K

T

S

K
|
K

Q

F

I

F

G

I

E

E

I

M

I

I

K

R

D

D

V

L

A

C

K

Q

V

A

S

D

G

K

I

T

-

W

N

N

S

V

V

V

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

T

G

G

A

A

H

H

P

A

S

S

V

G

N

C

I

I

G

G

E

E

L

D

P

P

L

Q

G

G

V

I

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

S

Q

Q

T

V

A

A

I

I

G

G

I

R

R

R

E

E

Q

A

L

L

S
x
N

V
|
V

F
|
F

G

G

N

N

D

D

Y

Y

P

D

T

T

T

E

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

K

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

-

D

-

K

-

K

K

N

E

A

Q

E

C

K

G

V

C

E

R

V

I

F

Y

N

N

L

L

G

G

T

T

G

G

K

T

G

G

N

Y

S

S

V
|
V

L

L

E

Q

V

M

I

V

N

Q

A

A

F

M

E

E

R

K

V

A

S

S

G

G

Q

K

K

K

L

I

N

P

Y

Y

K

K

I

V

V

V

D

A

R

R

R
|
R

A

E

G

G

D
|
D

V

V

A

A

E

A

A

C

Y

Y

A

A

D

N

T

P

A

S

K

L

A

A

N

Q

N

E

V

E

L

L

G

G

W

W

K

T

A

A

E

A

L

L

S

G

L

L

D

D

D

R

A

M

L

C

A

E

S

D

A

L

W

W

K

R

W

W

E

Q

Q

K

K

Q

active site: S131 (= S125), A132 (≠ C126), T133 (= T127), Y156 (= Y150), K160 (= K154)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), H35 (≠ S34), N36 (= N35), D65 (= D58), I66 (≠ L59), F87 (= F81), A88 (= A82), G89 (≠ A83), K91 (= K85), S129 (= S123), S131 (= S125), Y156 (= Y150), K160 (= K154), Y184 (= Y177), P187 (= P180)
binding uridine-5'-diphosphate-glucose: F185 (= F178), N186 (= N179), N206 (= N199), L207 (= L200), N223 (≠ S216), V224 (= V217), F225 (= F218), R238 (= R231), Y240 (= Y233), V276 (= V272), R299 (= R295), D302 (= D298)

8wovB Crystal structure of arabidopsis thaliana udp-glucose 4-epimerase 2 (atuge2) complexed with udp, g233a mutant (see paper)
49% identity, 97% coverage: 3:331/338 of query aligns to 5:333/341 of 8wovB

query
sites
8wovB

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

E

E

G

G

G

F

Y

D

S

V

A

V

V

I

V

D
|
D

D
x
N

L

Y

S
x
D

N
|
N

S
|
S

S

S

L

A

D

A

V

S

L

L

N

Q

G

R

I

V

E

K

S

K

I

L

T

A

G

G

K

E

-

N

-

G

-

N

K

R

P

L

E

S

F

F

E

H

K

Q

I

V

D
|
D

L
|
L

R

R

E

D

K

R

Q

P

A

A

V

L

K

E

D

K

F

I

F

F

V

S

K

E

Y

-

P

T

D

K

V

F

T

D

G

A

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

E

N

K

P

P

L

L

Y

Y

E

N

N
|
N

N

N

I

I

G

V

S

G

L

T

V

V

Y

T

L

L

Q

E

E

V

L

M

Q

A

K

Q

K

Y

Q

G

E

C

F

K

N

N

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C

A

T

T

V

V

Y

Y

G

G

Q

W

A

P

E

K

K

E

M

V

P

P

I

C

T

T

E

E

G

E

A

S

P

P

I

I

Q

S

G

-

A

A

L

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

I

F

G

I

E

E

I

I

I

C

K

R

D

D

V

V

A

H

K

R

V

S

-

D

S

S

G

E

I

W

N

K

S

I

V

I

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

Y

I

I

G

G

E

E

L

D

P

P

L

L

G

G

V

V

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

A

A

I

V

G

G

I

R

R

R

E

P

Q

H

L
|
L

S
x
T

V

V

F
|
F

G

G

N

T

D

D

Y

Y

P

K

T

T

T

K

D

D

G

G

T

T

C

A

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

M

D

D

L

L

A

A

K

D

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

D

D

D

K

L

K

K

N

I

A

S

E

-

K

-

V

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

K

N

G

G

N

T

S

S

V

V

L

L

E

E

V

M

I

V

N

A

A

A

F

F

E

E

R

K

V

A

S

S

G

G

Q

K

K

K

L

I

N

P

Y

L

K

V

I

M

V

A

D

G

R

R

R
|
R

A

P

G

G

D
|
D

V

A

A

E

E

V

A

V

Y

Y

A

A

D

S

T

T

A

E

K

K

A

A

N

E

N

R

V

E

L

L

G

N

W

W

K

K

A

A

E

K

L

N

S

G

L

I

D

E

A

M

L

C

A

R

S

D

A

L

W

W

K

N

W

W

binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ D32), D36 (≠ S34), N37 (= N35), S38 (= S36), D63 (= D58), L64 (= L59), F85 (= F81), A86 (= A82), G87 (≠ A83), K89 (= K85), N104 (= N100), S127 (= S123), S129 (= S125), Y153 (= Y150), K157 (= K154), Y181 (= Y177), P184 (= P180)
binding uridine-5'-diphosphate: N183 (= N179), N203 (= N199), L204 (= L200), L219 (= L215), T220 (≠ S216), F222 (= F218), R235 (= R231), Y237 (= Y233), R297 (= R295), D300 (= D298)

7kn1A Crystal structure of udp-glucose-4-epimerase (gale) from stenotrophomonas maltophila with bound NAD and formylated udp- arabinopyranose
46% identity, 99% coverage: 1:335/338 of query aligns to 3:333/336 of 7kn1A

query
sites
7kn1A

M

M

K

N

I

V

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

A

V

C

V

V

E

E

L

L

Q

Q

N

Q

E

Q

G

G

F

H

D

G

V

V

V

I

I

V

D
|
D

D
x
S

L

L

S
x
C

N
|
N

S

S

S

D

L

A

D

S

V

V

L

V

N

E

G

R

I

I

E

G

S

R

I

I

T

T

G

G

K

T

K

A

P

P

E

V

F

F

E

V

K

Q

I

A

D
|
D

L
x
I

R

R

E

D

K

R

Q

P

A

R

V

M

K

A

D

A

F

L

F

M

V

Q

K

E

Y

H

P

A

D

-

V

I

T

D

G

A

I

V

I

L

H

H

F
|
F

A
|
A

S

L

K
|
K

A

S

V
|
V

G

G

E

E

S

S

V

Q

E

K

N

I

P

P

L

L

L

Q

Y

Y

Y

F

E

D

N

S

N

N

I

I

G

S

S

G

L

S

V

I

Y

A

L

L

Q

G

E

A

L

M

Q

Q

K

D

K

A

Q

G

E

V

F

Q

N

L

F

L

I

V

F

F

S
|
S

C

A

T

T

V

V

Y

Y

G

G

Q

N

A

Q

E

D

K

H

M

C

P

P

I

V

T

A

E

E

G

T

A

A

P

S

I

T

Q

-

G

C

A

A

L

M

S

T

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

I

V

G

V

E

E

E

Q

I

L

K

A

D

D

V

L

A

A

K

A

V

T

-

G

S

Q

G

D

I

L

N

H

S

I

V

A

L

T

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

A

S

S

V

A

N

L

I

I

G

G

E

E

L

L

P

P

L

H

G

G

V

T

P

P

L
x
S

N
|
N

L
|
L

V

M

P

P

F

Y

I

I

T

A

Q

Q

T

V

A

A

I

A

G

G

I

L

R

L

E

P

Q

E

L

V

S
x
Q

V
|
V

F
|
F

G

G

N

D

D

D

Y

Y

P

P

T

T

T

H

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

V

Q

D

D

L

V

A

A

K

S

A

A

H

H

V

V

L

L

A

A

L

L

Q

Q

R

F

L

L

I

R

D

D

K

Q

K

R

N

-

A

-

E

-

K

R

V

S

E

I

V

T

F

L

N

N

L

L

G

G

T

T

G

G

K

Q

G

G

N

H

S

S

V
|
V

L

L

E

E

V

L

I

I

N

Q

A

A

F

F

E

E

R

L

V

T

S

T

G

G

Q

V

K

R

L

V

N

P

Y

F

K

R

I

I

V

V

D

P

R

R

R
|
R

A

D

G

G

D
|
D

V

I

A

A

E

V

A

S

Y

F

A

A

D

D

T

A

A

S

K

L

A

A

N

L

N

R

V

E

L

L

G

G

W

W

K

K

A

A

E

R

L

H

S

D

L

L

D

T

D

D

A

M

L

C

A

R

S

D

A

T

W

W

K

K

W

W

E

Q

Q

R

K

A

V

M

active site: S126 (= S125), Y150 (= Y150), K154 (= K154)
binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), S34 (≠ D32), C36 (≠ S34), N37 (= N35), D60 (= D58), I61 (≠ L59), F82 (= F81), A83 (= A82), A84 (= A83), K86 (= K85), S124 (= S123), S125 (= S124), S126 (= S125), Y150 (= Y150), K154 (= K154), Y178 (= Y177), P181 (= P180)
binding UDP-4-deoxy-4-formamido-beta-L-arabinopyranose: V88 (= V87), S126 (= S125), Y150 (= Y150), N180 (= N179), S199 (≠ L198), N200 (= N199), L201 (= L200), Q217 (≠ S216), V218 (= V217), F219 (= F218), R232 (= R231), Y234 (= Y233), V270 (= V272), R293 (= R295), D296 (= D298)

7xpqA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glcnac (see paper)
48% identity, 98% coverage: 3:334/338 of query aligns to 4:337/343 of 7xpqA

query
sites
7xpqA

I

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

Q

E

Q

G

G

F

F

D

R

V

V

I

V

D
|
D

D
x
N

L

L

S

D

N
|
N

S

A

S

S

L

E

D

A

V

A

L

L

N

A

G

R

I

V

E

A

S

E

I

L

T

A

G

G

K

H

K

D

P

G

E

A

-

N

-

L

-

V

F

F

E

H

K

K

I

V

D
|
D

L
|
L

R

R

E

D

K

R

Q

H

A

A

V

L

K

V

D

D

F

I

F

F

V

S

K

S

Y

H

P

-

D

R

V

F

T

E

G

A

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

H

N

K

P

P

L

L

Y

Y

E

D

N
|
N

N

N

I

L

G

V

S

G

L

T

V

I

Y

T

L

L

Q

E

E

V

L

M

Q

A

K

A

K

N

Q

G

E

C

F

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C

A

T

T

V

V

Y

Y

G

G

Q

W

A

P

E

K

K

E

M

V

P

P

I

C

T

T

E

E

G

E

A

F

P

P

I

L

Q

C

G

-

A

A

L

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

I

V

G

I

E

E

E

D

I

I

I

C

K

R

D

D

V

V

A

H

K

R

V

S

S

D

-

P

G

D

I

W

N

K

S

I

V

I

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

Y

I

I

G

G

E

E

L

D

P

P

L

C

G

G

V

V

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

A

A

I

V

G

G

I

R

R

L

E

P

Q

H

L

L

S
x
T

V

V

F
x
Y

G

G

N

T

D

D

Y

Y

P

S

T

T

T

K

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

D

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

Y

D

E

K

D

K

S

N

D

A

K

E

I

K

G

V

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

K

K

G

G

N

T

S

S

V
|
V

L

L

E

E

V

M

I

V

N

A

A

A

F

F

E

E

R

K

V

A

S

S

G

G

Q

K

K

K

L

I

N

P

Y

L

K

V

I

F

V

A

D

G

R

R

R
|
R

A

P

G

G

D
|
D

V

A

A

E

E

I

A

V

Y

Y

A

A

D

A

T

T

A

A

K

K

A

A

N

E

N

K

V

E

L

L

G

K

W

W

K

K

A

A

E

K

L

Y

S

G

L

I

D

E

A

M

L

C

A

R

S

D

A

L

W

W

K

N

W

W

E

A

Q

S

K

K

binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), N36 (= N35), D62 (= D58), L63 (= L59), F84 (= F81), A85 (= A82), G86 (≠ A83), K88 (= K85), N103 (= N100), S126 (= S123), S128 (= S125), Y152 (= Y150), K156 (= K154), Y180 (= Y177), P183 (= P180)
binding uridine-diphosphate-n-acetylglucosamine: N182 (= N179), N202 (= N199), L203 (= L200), T219 (≠ S216), Y221 (≠ F218), R234 (= R231), Y236 (= Y233), V275 (= V272), R298 (= R295), D301 (= D298)

7xpoA Crystal structure of udp-glc/glcnac 4-epimerase with NAD/udp-glc (see paper)
48% identity, 98% coverage: 3:334/338 of query aligns to 4:337/344 of 7xpoA

query
sites
7xpoA

I

I

L

L

V

V

T

T

G
|
G

G

G

L

A

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

H

T

T

V

V

V

L

E

Q

L

L

Q

L

N

Q

E

Q

G

G

F

F

D

R

V

V

I

V

D
|
D

D
x
N

L

L

S

D

N
|
N

S

A

S

S

L

E

D

A

V

A

L

L

N

A

G

R

I

V

E

A

S

E

I

L

T

A

G

G

K

H

K

D

P

G

E

A

-

N

-

L

-

V

F

F

E

H

K

K

I

V

D
|
D

L
|
L

R

R

E

D

K

R

Q

H

A

A

V

L

K

V

D

D

F

I

F

F

V

S

K

S

Y

H

P

-

D

R

V

F

T

E

G

A

I

V

I

I

H

H

F
|
F

A
|
A

A
x
G

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

V

E

H

N

K

P

P

L

L

Y

Y

E

D

N
|
N

N

N

I

L

G

V

S

G

L

T

V

I

Y

T

L

L

Q

E

E

V

L

M

Q

A

K

A

K

N

Q

G

E

C

F

K

N

K

F

L

I

V

F

F

S
|
S

S

S

S
|
S

C
x
A

T

T

V

V

Y

Y

G

G

Q

W

A

P

E

K

K

E

M

V

P

P

I

C

T

T

E

E

G

E

A

F

P

P

I

L

Q

C

G

-

A

A

L

T

S

N

P

P

Y
|
Y

G

G

N

R

T

T

K
|
K

Q

L

I

V

G

I

E

E

E

D

I

I

I

C

K

R

D

D

V

V

A

H

K

R

V

S

S

D

-

P

G

D

I

W

N

K

S

I

V

I

L

L

R

R

Y
|
Y

F
|
F

N
|
N

P
|
P

V

V

G

G

A

A

H

H

P

P

S

S

V

G

N

Y

I

I

G

G

E

E

L

D

P

P

L

C

G

G

V

V

P

P

L

N

N
|
N

L
|
L

V

M

P

P

F

Y

I

V

T

Q

Q

Q

T

V

A

A

I

V

G

G

I

R

R

L

E

P

Q

H

L

L

S
x
T

V
|
V

F
x
Y

G

G

N

T

D

D

Y

Y

P

S

T

T

T

K

D

D

G

G

T

T

C

G

V

V

R
|
R

D

D

Y
|
Y

I

I

H

H

V

V

D

D

L

L

A

A

K

D

A

G

H

H

V

I

L

A

A

A

L

L

Q

R

R

K

L

L

I

Y

D

E

K

D

K

S

N

D

A

K

E

I

K

G

V

C

E

E

V

V

F

Y

N

N

L

L

G

G

T

T

G

G

K

K

G

G

N

T

S

S

V
|
V

L

L

E

E

V

M

I

V

N

A

A

A

F

F

E

E

R

K

V

A

S

S

G

G

Q

K

K

K

L

I

N

P

Y

L

K

V

I

F

V

A

D

G

R

R

R
|
R

A

P

G

G

D
|
D

V

A

A

E

E

I

A

V

Y

Y

A

A

D

A

T

T

A

A

K

K

A

A

N

E

N

K

V

E

L

L

G

K

W

W

K

K

A

A

E

K

L

Y

S

G

L

I

D

E

A

M

L

C

A

R

S

D

A

L

W

W

K

N

W

W

E

A

Q

S

K

K

binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), N36 (= N35), D62 (= D58), L63 (= L59), F84 (= F81), A85 (= A82), G86 (≠ A83), K88 (= K85), N103 (= N100), S126 (= S123), S128 (= S125), Y152 (= Y150), K156 (= K154), Y180 (= Y177), P183 (= P180)
binding uridine-5'-diphosphate-glucose: S128 (= S125), A129 (≠ C126), F181 (= F178), N182 (= N179), N202 (= N199), L203 (= L200), T219 (≠ S216), V220 (= V217), Y221 (≠ F218), R234 (= R231), Y236 (= Y233), V275 (= V272), R298 (= R295), D301 (= D298)

4lisB Crystal structure of udp-galactose-4-epimerase from aspergillus nidulans (see paper)
47% identity, 98% coverage: 3:333/338 of query aligns to 4:349/363 of 4lisB

query
sites
4lisB

I

V

L

L

V

V

T

T

G

G

L

T

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

F

T

T

V

T

V

L

E

A

L

L

Q

L

N

E

E

A

G

G

F

Y

D

K

V

V

I

A

D
|
D

D
x
N

L

L

S

Y

N
|
N

S
|
S

S

S

L

A

D

E

V

A

L

L

N

N

G

R

I

I

E

E

S

L

I

I

T

S

G

G

K

K

P

A

E

E

F

F

E

A

K

Q

I

L

D
|
D

L
x
V

R

T

E

D

K

E

Q

A

A

A

V

F

K

D

D

K

F

V

F

F

V

E

K

A

Y

H

P

P

D

D

V

I

T

D

G

S

I

V

I

I

H

H

F
|
F

A

A

A
|
A

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

G

E

E

N

K

P

P

L

L

L

D

Y

Y

E

H

N

V

N

N

I

V

G

Y

S

G

L

T

V

I

Y

C

L

L

Q

R

E

S

L

M

Q

V

K

R

K

H

Q

N

E

V

F

T

N

N

F

I

I

V

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

A

E

T

K

R

-

F

-

P

-

D

-

M

M

I

P

P

I

I

T

P

E

E

G

H

A

C

P

P

I

L

Q

-

G

G

A

P

L

T

S

N

P

P

Y
|
Y

G

G

N

N

T

T

K
|
K

Q

F

I

A

G

I

E

E

E

L

I

A

I

I

K

T

D

D

V

V

-

I

-

N

-

A

-

Q

-

R

-

N

A

A

K

K

V

K

S

A

G

G

-

N

-

E

-

T

-

E

-

A

-

K

I

W

N

N

S

G

V

A

L

L

R

R

Y
|
Y

F

F

N
|
N

P
|
P

V

A

G

G

A

A

H

H

P

P

S

S

V

G

N

I

I

M

G

G

E

E

L

D

P

P

L

Q

G

G

V

V

P

P

L

Y

N
|
N

L
|
L

V

L

P

P

F

L

I

L

T

A

Q

Q

T

V

A

A

I

T

G

G

I

K

R

R

E

E

Q

K

L

L

S
x
L

V
|
V

F
|
F

G

G

N

D

D

D

Y

Y

P

A

T

S

T

H

D

D

G

G

T

T

C

A

V

I

R
|
R

D

D

Y

Y

I

I

H

H

V

I

V

L

D

D

L

L

A

A

K

D

A

G

H

H

V

L

L

K

A

A

L

L

Q

N

R

Y

L

L

I

-

D

-

K

R

K

A

N

N

A

N

E

P

K

G

V

V

E

R

V

A

F

W

N

N

L

L

G

G

T

T

G

G

K

R

G

G

N

S

S

T

V
|
V

L

Y

E

E

V

M

I

I

N

R

A

A

F

F

E

S

R

K

V

A

S

V

G

G

Q

R

K

D

L

L

N

P

Y

Y

K

E

I

V

V

A

D

P

R

R

R
|
R

A

A

G

G

D
|
D

V

V

A

L

E

N

A

L

Y

T

A

S

D

N

T

P

A

T

K

R

A

A

N

N

N

T

V

E

L

L

G

G

W

W

K

K

A

A

E

Q

L

R

S

T

L

L

D

E

D

Q

A

A

L

C

A

E

S

D

A

L

W

W

K

L

W

W

E

T

Q

K

active site: S126 (= S125), A127 (≠ C126), T128 (= T127), Y154 (= Y150), K158 (= K154)
binding nicotinamide-adenine-dinucleotide: G11 (= G10), Y12 (≠ F11), I13 (= I12), D32 (= D31), N33 (≠ D32), N36 (= N35), S37 (= S36), D59 (= D58), V60 (≠ L59), F82 (= F81), A84 (= A83), K86 (= K85), S126 (= S125), Y154 (= Y150), K158 (= K154), Y195 (= Y177), P198 (= P180)
binding uridine-5'-diphosphate: N197 (= N179), N217 (= N199), L218 (= L200), L234 (≠ S216), V235 (= V217), F236 (= F218), R249 (= R231), V288 (= V272), R311 (= R295), D314 (= D298)

4lisA Crystal structure of udp-galactose-4-epimerase from aspergillus nidulans (see paper)
47% identity, 98% coverage: 3:333/338 of query aligns to 5:350/364 of 4lisA

query
sites
4lisA

I

V

L

L

V

V

T

T

G

G

L

T

G
|
G

F
x
Y

I
|
I

G

G

S

S

H

F

T

T

V

T

V

L

E

A

L

L

Q

L

N

E

E

A

G

G

F

Y

D

K

V

V

I

A

D
|
D

D
x
N

L

L

S

Y

N
|
N

S
|
S

S

S

L

A

D

E

V

A

L

L

N

N

G

R

I

I

E

E

S

L

I

I

T

S

G

G

K

K

P

A

E

E

F

F

E

A

K

Q

I

L

D
|
D

L
x
V

R

T

E

D

K

E

Q

A

A

A

V

F

K

D

D

K

F

V

F

F

V

E

K

A

Y

H

P

P

D

D

V

I

T

D

G

S

I

V

I

I

H

H

F
|
F

A

A

A
|
A

S

L

K
|
K

A

A

V

V

G

G

E

E

S

S

V

G

E

E

N

K

P

P

L

L

L

D

Y

Y

E

H

N

V

N

N

I

V

G

Y

S

G

L

T

V

I

Y

C

L

L

Q

R

E

S

L

M

Q

V

K

R

K

H

Q

N

E

V

F

T

N

N

F

I

I

V

F

F

S

S

S
|
S

C
x
A

T
|
T

V

V

Y

Y

G

G

Q

D

A

A

E

T

K

R

-

F

-

P

-

D

-

M

M

I

P

P

I

I

T

P

E

E

G

H

A

C

P

P

I

L

Q

-

G

G

A

P

L

T

S

N

P

P

Y
|
Y

G

G

N

N

T

T

K
|
K

Q

F

I

A

G

I

E

E

E

L

I

A

I

I

K

T

D

D

V

V

-

I

-

N

-

A

-

Q

-

R

-

N

A

A

K

K

V

K

S

A

G

G

-

N

-

E

-

T

-

E

-

A

-

K

I

W

N

N

S

G

V

A

L

L

R

R

Y
|
Y

F

F

N

N

P
|
P

V

A

G

G

A

A

H

H

P

P

S

S

V

G

N

I

I

M

G

G

E

E

L

D

P

P

L

Q

G

G

V

V

P

P

L

Y

N
|
N

L

L

V

L

P

P

F

L

I

L

T

A

Q

Q

T

V

A

A

I

T

G

G

I

K

R

R

E

E

Q

K

L

L

S
x
L

V
|
V

F
|
F

G

G

N

D

D

D

Y

Y

P

A

T

S

T

H

D

D

G

G

T

T

C

A

V

I

R
|
R

D

D

Y

Y

I

I

H

H

V

I

V

L

D

D

L

L

A

A

K

D

A

G

H

H

V

L

L

K

A

A

L

L

Q

N

R

Y

L

L

I

-

D

-

K

R

K

A

N

N

A

N

E

P

K

G

V

V

E

R

V

A

F

W

N

N

L

L

G

G

T

T

G

G

K

R

G

G

N

S

S

T

V

V

L

Y

E

E

V

M

I

I

N

R

A

A

F

F

E

S

R

K

V

A

S

V

G

G

Q

R

K

D

L

L

N

P

Y

Y

K

E

I

V

V

A

D

P

R

R

R
|
R

A

A

G

G

D
|
D

V

V

A

L

E

N

A

L

Y

T

A

S

D

N

T

P

A

T

K

R

A

A

N

N

N

T

V

E

L

L

G

G

W

W

K

K

A

A

E

Q

L

R

S

T

L

L

D

E

D

Q

A

A

L

C

A

E

S

D

A

L

W

W

K

L

W

W

E

T

Q

K

active site: S127 (= S125), A128 (≠ C126), T129 (= T127), Y155 (= Y150), K159 (= K154)
binding nicotinamide-adenine-dinucleotide: G12 (= G10), Y13 (≠ F11), I14 (= I12), D33 (= D31), N34 (≠ D32), N37 (= N35), S38 (= S36), D60 (= D58), V61 (≠ L59), F83 (= F81), A85 (= A83), K87 (= K85), S127 (= S125), Y155 (= Y150), K159 (= K154), Y196 (= Y177), P199 (= P180)
binding uridine-5'-diphosphate-glucose: K87 (= K85), S127 (= S125), Y155 (= Y150), N218 (= N199), L235 (≠ S216), V236 (= V217), F237 (= F218), R250 (= R231), R312 (= R295), D315 (= D298)

Query Sequence

>WP_035135055.1 NCBI__GCF_000769915.1:WP_035135055.1
MKILVTGGLGFIGSHTVVELQNEGFDVVVIDDLSNSSLDVLNGIESITGKKPEFEKIDLR
EKQAVKDFFVKYPDVTGIIHFAASKAVGESVENPLLYYENNIGSLVYLLQELQKKQEFNF
IFSSSCTVYGQAEKMPITEGAPIQGALSPYGNTKQIGEEIIKDVAKVSGINSVLLRYFNP
VGAHPSVNIGELPLGVPLNLVPFITQTAIGIREQLSVFGNDYPTTDGTCVRDYIHVVDLA
KAHVLALQRLIDKKNAEKVEVFNLGTGKGNSVLEVINAFERVSGQKLNYKIVDRRAGDVA
EAYADTAKANNVLGWKAELSLDDALASAWKWEQKVRGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory