SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035135395.1 NCBI__GCF_000769915.1:WP_035135395.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P09148 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Escherichia coli (strain K12) (see 4 papers)
52% identity, 99% coverage: 4:340/340 of query aligns to 5:339/348 of P09148

query
sites
P09148

N

N

L

P

N

V

E

D

Y

H

P

P

H

H

R

R

Y

Y

N

N

A

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

S
x
A

K
|
K

R
|
R

P

P

W

W

Q

Q

G

G

Q

A

V

Q

E

E

K

T

H

P

S

A

A

K

E

Q

V

V

R

L

P

P

Q

A

Y

H

D

D

P

P

G

D

C
|
C

Y

F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

S
x
V

G

T

G

G

E

D

Q

K

N

N

P

P

D

D

Y

Y

K

T

D

G

V

T

Y

Y

V

V

F

F

I

T

N
|
N

D
|
D

F

F

S

A

A

A

L

L

L

M

K

S

D

D

S

T

P

P

D

D

F

A

D

P

V

E

V

S

E

H

D

D

D

P

I

L

F

M

K

R

A

C

E

Q

S

S

E

A

K

R

G

G

I

T

C

S

R

R

V

V

I

I

C

C

F

F

S

S

P

P

D

D

H
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H

S

S

L

K

T

T

I

L

P

P

Q

E

M

L

K

S

V

V

E

A

D

A

I

L

R

T

K

E

V

I

V

V

D

K

L

T

W

W

I

Q

E

E

E

Q

Y

T

H

A

T

E

L

L

G

G

S

K

D

T

P

-

E

-

I

Y

N

P

Y

W

V

V

Q

Q

I

V

F

F

E

E

N
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N

K

K

G

G

E

A

I

A

M

M

G
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G

C
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C

S
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S

N

N

P

P

H
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H

P

P

H
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H

G

G

Q
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Q

I

I

W

W

A

A

S

N

Q

S

K

F

L

L

P

P

V

N

E

E

P

A

S

E

K

R

K
x
E

Q

D

H

R

Y

L

Q

Q

L

K

E

E

Y

Y

F

F

Q

A

K

E

H

Q

G

K

D

S

T

P

L

M

L

L

S

V

D

D

Y

Y

L

V

Q

Q

K

R

E

E

L

L

Q

A

K

D

D

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

N

E

H

H

F

W

A

L

A

A

I

V

V

V

P

P

F

Y

W

W

A

A

T

A

W

W

P

P

Y

F

E

E

A

T

M

L

I

L

P

P

K

K

R

A

A

H

M

V

A

L

R

R

I

I

T

T

D

D

M

L

T

T

D

D

D

A

E

Q

K

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T

S

A

D

L

L

A

A

D

L

T

A

Y

L

K

K

R

K

L

L

T

T

M

S

I

R

Y

Y

D

D

K

N

V

L

F

F

D

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
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H

Q

G

A

A

P

P

T

F

D

N

G

G

K

E

E

E

H

N

N

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

A
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
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Y

E
|
E

M

M

L

L

A

A

T

E

P

T

Q
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Q

R

R

D

D

I

L

T

T

P

A

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

V

N

S

RAKR 28:31 (≠ RSKR 27:30) binding UDP-alpha-D-glucose
C52 (= C51) binding Zn(2+); mutation to S: Decreases enzyme activity 3000-fold.
C55 (= C54) binding Zn(2+); mutation to S: Decreases enzyme activity 600-fold.
V61 (≠ S60) binding in other chain
ND 77:78 (= ND 76:77) binding in other chain
H115 (= H114) binding Zn(2+); mutation to N: Decreases enzyme activity by 98%.
N153 (= N154) binding in other chain
GCS 159:161 (= GCS 160:162) binding in other chain
C160 (= C161) mutation C->S,A: Slight inhibition of enzymatic activity.
S161 (= S162) mutation to A: 7000-fold reduction in specific activity.
H164 (= H165) binding Zn(2+); mutation to N: Decreases enzyme activity 10000-fold.
H166 (= H167) active site, Tele-UMP-histidine intermediate; mutation to G: Abolishes enzymatic activity.
Q168 (= Q169) binding in other chain
E182 (≠ K183) binding Fe cation; mutation to A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding.
H281 (= H282) binding Fe cation
H296 (= H297) binding Fe cation
H298 (≠ A299) binding Fe cation
KF 311:312 (= KF 312:313) binding UDP-alpha-D-glucose
YE 316:317 (= YE 317:318) binding UDP-alpha-D-glucose
Q323 (= Q324) binding in other chain

1guqA Structure of nucleotidyltransferase complexed with udp-glucose (see paper)
51% identity, 99% coverage: 4:340/340 of query aligns to 4:338/347 of 1guqA

query
sites
1guqA

N

N

L

P

N

V

E

D

Y

H

P

P

H

H

R

R

Y

Y

N

N

A

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

S

A

K

K

R
|
R

P

P

W
|
W

Q

Q

G

G

Q

A

V

Q

E

E

K

T

H

P

S

A

A

K

E

Q

V

V

R

L

P

P

Q

A

Y

H

D

D

P

P

G

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

S
x
V

G

T

G

G

E

D

Q

K

N

N

P

P

D

D

Y

Y

K

T

D

G

V

T

Y

Y

V

V

F

F

I

T

N
|
N

D
|
D

F

F

S

A

A

A

L

L

L

M

K

S

D

D

S

T

P

P

D

D

F

A

D

P

V

E

V

S

E

H

D

D

D

P

I

L

F

M

K

R

A

C

E

Q

S

S

E

A

K

R

G

G

I

T

C

S

R

R

V

V

I

I

C

C

F

F

S

S

P

P

D

D

H
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H

S

S

L

K

T

T

I

L

P

P

Q

E

M

L

K

S

V

V

E

A

D

A

I

L

R

T

K

E

V

I

V

V

D

K

L

T

W

W

I

Q

E

E

E

Q

Y

T

H

A

T

E

L

L

G

G

S

K

D

T

P

-

E

-

I

Y

N

P

Y

W

V

V

Q

Q

I

V

F
|
F

E
|
E

N
|
N

K
|
K

G

G

E

A

I

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W

W

A

A

S

N

Q

S

K

F

L

L

P

P

V

N

E

E

P

A

S

E

K

R

K
x
E

Q

D

H

R

Y

L

Q

Q

L

K

E

E

Y

Y

F

F

Q

A

K

E

H

Q

G

K

D

S

T

P

L

M

L

L

S

V

D

D

Y

Y

L

V

Q

Q

K

R

E

E

L

L

Q

A

K

D

D

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

N

E

H

H

F

W

A

L

A

A

I

V

V

V

P

P

F

Y

W

W

A

A

T

A

W

W

P

P

Y

F

E

E

A

T

M

L

I

L

P

P

K

K

R

A

A

H

M

V

A

L

R

R

I

I

T

T

D

D

M

L

T

T

D

D

D

A

E

Q

K

R

T

S

A

D

L

L

A

A

D

L

T

A

Y

L

K

K

R

K

L

L

T

T

M

S

I

R

Y

Y

D

D

K

N

V

L

F

F

D

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
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H

Q

G

A

A

P

P

T

F

D

N

G

G

K

E

E

E

H

N

N

Q

E

H

W

W

H

Q

W

L

H
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H

M

A

A
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
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Y

E
|
E

M

M

L

L

A

A

T

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

V

N

S

active site: C51 (= C51), C54 (= C54), H114 (= H114), N152 (= N154), S160 (= S162), H163 (= H165), G165 (≠ H167), Q167 (= Q169)
binding fe (iii) ion: E181 (≠ K183), H280 (= H282), H295 (= H297), H297 (≠ A299)
binding potassium ion: E151 (= E153), N152 (= N154), K153 (= K155), G165 (≠ H167)
binding uridine-5'-diphosphate-glucose: R27 (= R27), R30 (= R30), W32 (= W32), F52 (≠ Y52), V60 (≠ S60), N76 (= N76), D77 (= D77), F150 (= F152), N152 (= N154), G158 (= G160), C159 (= C161), S160 (= S162), Q167 (= Q169), K310 (= K312), F311 (= F313), Y315 (= Y317), E316 (= E318)
binding zinc ion: C51 (= C51), C54 (= C54), H114 (= H114), H163 (= H165)

1gupA Structure of nucleotidyltransferase complexed with udp-galactose (see paper)
51% identity, 99% coverage: 4:340/340 of query aligns to 4:338/347 of 1gupA

query
sites
1gupA

N

N

L

P

N

V

E

D

Y

H

P

P

H

H

R

R

Y

Y

N

N

A

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

S

A

K

K

R
|
R

P

P

W

W

Q

Q

G

G

Q

A

V

Q

E

E

K

T

H

P

S

A

A

K

E

Q

V

V

R

L

P

P

Q

A

Y

H

D

D

P

P

G

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R
|
R

S
x
V

G

T

G

G

E

D

Q

K

N

N

P

P

D

D

Y

Y

K

T

D

G

V

T

Y

Y

V

V

F

F

I

T

N
|
N

D
|
D

F
|
F

S

A

A

A

L

L

L

M

K

S

D

D

S

T

P

P

D

D

F

A

D

P

V

E

V

S

E

H

D

D

D

P

I

L

F

M

K

R

A

C

E

Q

S

S

E

A

K

R

G

G

I

T

C

S

R

R

V

V

I

I

C

C

F

F

S

S

P

P

D

D

H
|
H

S

S

L

K

T

T

I

L

P

P

Q

E

M

L

K

S

V

V

E

A

D

A

I

L

R

T

K

E

V

I

V

V

D

K

L

T

W

W

I

Q

E

E

E

Q

Y

T

H

A

T

E

L

L

G

G

S

K

D

T

P

-

E

-

I

Y

N

P

Y

W

V

V

Q

Q

I

V

F
|
F

E

E

N
|
N

K
|
K

G

G

E

A

I

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W
|
W

A

A

S

N

Q

S

K

F

L

L

P

P

V

N

E

E

P

A

S

E

K

R

K
x
E

Q

D

H

R

Y

L

Q

Q

L

K

E

E

Y

Y

F

F

Q

A

K

E

H

Q

G

K

D

S

T

P

L

M

L

L

S

V

D

D

Y

Y

L

V

Q

Q

K

R

E

E

L

L

Q

A

K

D

D

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

N

E

H

H

F

W

A

L

A

A

I

V

V

V

P

P

F

Y

W

W

A

A

T

A

W

W

P

P

Y

F

E

E

A

T

M

L

I

L

P

P

K

K

R

A

A

H

M

V

A

L

R

R

I

I

T

T

D

D

M

L

T

T

D

D

D

A

E

Q

K

R

T

S

A

D

L

L

A

A

D

L

T

A

Y

L

K

K

R

K

L

L

T

T

M

S

I

R

Y

Y

D

D

K

N

V

L

F

F

D

Q

V

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

F

D

N

G

G

K

E

E

E

H

N

N

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

A
x
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K
|
K

F
|
F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

T

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

V

N

S

active site: C51 (= C51), C54 (= C54), H114 (= H114), N152 (= N154), S160 (= S162), H163 (= H165), G165 (≠ H167), Q167 (= Q169)
binding fe (iii) ion: E181 (≠ K183), H280 (= H282), H295 (= H297), H297 (≠ A299)
binding galactose-uridine-5'-diphosphate: R27 (= R27), R30 (= R30), F52 (≠ Y52), R59 (= R59), V60 (≠ S60), N76 (= N76), D77 (= D77), F78 (= F78), F150 (= F152), N152 (= N154), G158 (= G160), C159 (= C161), S160 (= S162), Q167 (= Q169), W169 (= W171), K310 (= K312), F311 (= F313), V313 (= V315), G314 (= G316), E316 (= E318)
binding potassium ion: N152 (= N154), K153 (= K155), G165 (≠ H167)
binding zinc ion: C51 (= C51), C54 (= C54), H114 (= H114), H163 (= H165)

1hxpA Nucleotide transferase (see paper)
51% identity, 99% coverage: 4:340/340 of query aligns to 4:331/340 of 1hxpA

query
sites
1hxpA

N

N

L

P

N

V

E

D

Y

H

P

P

H

H

R

R

Y

Y

N

N

A

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R

R

S

A

K

K

R

R

P

P

W

W

Q

E

G

G

Q

A

V

V

E

-

K

-

H

-

S

-

A

-

E

-

V

-

R

L

P

P

Q

A

Y

H

D

D

P

P

G

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

S
x
V

G

T

G

G

E

D

Q

K

N

N

P

P

D

D

Y

Y

K

T

D

G

V

T

Y

Y

V

V

F

F

I

T

N
|
N

D
|
D

F

F

S

A

A

A

L

L

L

M

K

S

D

D

S

T

P

P

D

D

F

A

D

P

V

E

V

S

E

H

D

D

D

P

I

L

F

M

K

R

A

C

E

Q

S

S

E

A

K

R

G

G

I

T

C

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R

R

V

V

I

I

C

C

F

F

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D

D

H
|
H

S

S

L

K

T

T

I

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P

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V

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A

D

A

I

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T

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I

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V

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W

I

Q

E

E

E

Q

Y

T

H

A

T

E

L

L

G

G

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D

T

P

-

E

-

I

Y

N

P

Y

W

V

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

E

A

I

A

M

M

G

G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
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H

G

G

Q
|
Q

I

I

W

W

A

A

S

N

Q

S

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F

L

L

P

P

V

N

E

E

P

A

S

E

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x
E

Q

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Q

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Y

F

F

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A

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D

D

Y

Y

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E

L

L

Q

A

K

D

D

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

N

E

H

H

F

W

A

L

A

A

I

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V

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P

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A

A

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A

W

W

P

P

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F

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E

A

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I

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P

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A

A

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A

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T

D

D

D

A

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A

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A

A

D

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T

A

Y

L

K

K

R

K

L

L

T

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M

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Y

Y

D

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N

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L

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F

D

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x
C

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|
S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
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H

Q

G

A

A

P

P

T

F

D

N

G

G

K

E

E

E

H

N

N

Q

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H

W

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H

M

A

A
x
H

F

F

Y

Y

P

P

L
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L

L

L

R

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S

A
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A

T

T

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R

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K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

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L

A

A

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T

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Q

R

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D

D

I

L

T

T

P

A

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

V

N

S

active site: C44 (= C51), C47 (= C54), H107 (= H114), N145 (= N154), S153 (= S162), H156 (= H165), H158 (= H167), Q160 (= Q169)
binding beta-mercaptoethanol: N145 (= N154), C152 (= C161), Q160 (= Q169), C264 (≠ V273), S265 (= S274), L295 (= L304), A299 (= A308)
binding fe (iii) ion: E174 (≠ K183), H273 (= H282), H288 (= H297), H290 (≠ A299)
binding uridine-5'-monophosphate: F45 (≠ Y52), V53 (≠ S60), N69 (= N76), D70 (= D77)
binding zinc ion: C44 (= C51), C47 (= C54), H107 (= H114), H156 (= H165)

1hxpB Nucleotide transferase (see paper)
49% identity, 99% coverage: 4:340/340 of query aligns to 4:322/329 of 1hxpB

query
sites
1hxpB

N

N

L
x
P

N

V

E

D

Y

H

P

P

H
|
H

R

R

Y

Y

N

N

A

P

L

L

T

T

G

G

E

Q

W

W

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I

L

L

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V

S

S

P

P

H

H

R

R

S

-

K

-

R

-

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-

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-

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-

G

-

Q

-

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-

E

-

K

-

H

-

S

-

A

-

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-

V

-

R

L

P

P

Q

A

Y

H

D

D

P

P

G

D

C
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C

Y

F

L

L

C
|
C

P

A

G

G

N

N

S

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R
|
R

S
x
V

G

T

G

G

E

D

Q

K

N

N

P

P

D

D

Y

Y

K

T

D

G

V

T

Y

Y

V

V

F
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F

I

T

N
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N

D
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D

F

F

S

A

A

A

L

L

L

M

K

S

D

D

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A

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A

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G

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-

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N

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C

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S

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N

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A

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Y

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x
H

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active site: C35 (= C51), C38 (= C54), H98 (= H114), N136 (= N154), S144 (= S162), H147 (= H165), H149 (= H167), Q151 (= Q169)
binding beta-mercaptoethanol: P5 (≠ L5), H10 (= H10), N136 (= N154), C143 (= C161), Q151 (= Q169), Y208 (≠ F226)
binding fe (iii) ion: E165 (≠ K183), H264 (= H282), H279 (= H297), H281 (≠ A299)
binding uridine-5'-diphosphate: R43 (= R59), V44 (≠ S60), F58 (= F74), N60 (= N76), D61 (= D77), S144 (= S162), N145 (= N163)
binding zinc ion: C35 (= C51), C38 (= C54), H98 (= H114), H147 (= H165)

Sites not aligning to the query:

binding beta-mercaptoethanol: 3

P07902 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Homo sapiens (Human) (see 22 papers)
49% identity, 98% coverage: 6:339/340 of query aligns to 27:361/379 of P07902

query
sites
P07902

N

N

E

D

Y

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H

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x
I

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Y
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Y

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L

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S

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T

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M

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V

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Y

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V

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I

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F

E

E

N

N

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G

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M

M

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G

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P

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H

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L

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R

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I

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x
K

Y

Y

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N

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L

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x
E

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F

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Y

Y

S

S

A

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G

G

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H

Q

G

A

A

P

P

T

T

D

G

-

S

-

E

-

A

G

G

K

A

E

N

H

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N
|
N

E

H

W

W

H

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W

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H
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H

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A

A
x
H

F

Y

Y

Y

P

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L

L

L
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L

R

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S

A
|
A

T

T

V

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K
x
R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

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P

A

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Q

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D

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I

L

T
|
T

P

P

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

L

I32 (≠ R11) to N: in GALAC1; mild; dbSNP:rs111033644
Y34 (= Y13) to N: in GALAC1; affects protein stability; dbSNP:rs111033836
V44 (= V23) to M: in GALAC1; reduced enzyme activity; dbSNP:rs111033647
L62 (≠ A41) to M: in dbSNP:rs1800461
L74 (= L53) to P: in GALAC1; reduced enzyme activity; dbSNP:rs111033663
H132 (≠ S111) to Q: in GALAC1; affects protein stability; dbSNP:rs367543256
S135 (≠ H114) to L: in GALAC1; about 5% of normal galactose uridylyltransferase activity; dbSNP:rs111033690
T138 (= T117) to M: in GALAC1; mild; dbSNP:rs111033686
M142 (= M121) to K: in GALAC1; 4% of normal activity; dbSNP:rs111033695
R148 (= R127) to W: in GALAC1; unstable protein; dbSNP:rs111033693
V151 (= V130) to A: in GALAC1; approximately 3% of normal activity; dbSNP:rs111033701
V168 (= V149) to L: in GALAC1; loss of activity; dbSNP:rs367543258
I170 (= I151) to T: in GALAC1; loss of activity; dbSNP:rs111033839
F171 (= F152) to S: in GALAC1; reduced enzyme activity; dbSNP:rs111033715
G175 (= G156) to D: in GALAC1; strongly reduces galactose uridylyltransferase activity; dbSNP:rs111033718
P185 (= P166) to H: in GALAC1; loss of activity; dbSNP:rs111033722
H186 (= H167) active site, Tele-UMP-histidine intermediate
Q188 (= Q169) to R: in GALAC1; most common mutation; 10% of normal galactose uridylyltransferase activity; impairs protein folding; dbSNP:rs75391579
L195 (= L176) to P: in GALAC1; no enzymatic activity; dbSNP:rs111033728
R201 (≠ K182) to C: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033739
E202 (≠ K183) binding Zn(2+)
E220 (≠ D201) to K: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033747
R223 (≠ Q204) to S: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033750
L227 (≠ Q208) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033846
R231 (= R212) to H: in GALAC1; 15% of normal activity; dbSNP:rs111033754
R259 (= R240) to Q: in GALAC1; loss of activity; dbSNP:rs886042070; to W: in GALAC1; mild; dbSNP:rs786204763
I278 (≠ T259) to N: in GALAC1; 18-fold decrease in activity; dbSNP:rs111033778
K285 (≠ I266) to N: in GALAC1; severe; impairs protein folding; nearly abolishes enzyme activity; dbSNP:rs111033773
L289 (≠ V270) to F: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033774
E291 (≠ D272) to V: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033841
H301 (= H282) binding Zn(2+)
N314 (= N292) to D: in GALAC1; allele Duarte; exists as allelic variants Duarte-1 and Duarte-2; Duarte-1 has normal or increased activity; Duarte-2 has activity reduced to about 35-45% of normal; dbSNP:rs2070074
H319 (= H297) binding Zn(2+)
H321 (≠ A299) binding Zn(2+)
L327 (= L305) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033832
A330 (= A308) to V: in GALAC1; mild; dbSNP:rs111033804
R333 (≠ K311) to W: in GALAC1; no enzymatic activity; dbSNP:rs111033800
T350 (= T328) to A: in GALAC1; mild; dbSNP:rs111033817

6gqdA Structure of human galactose-1-phosphate uridylyltransferase (galt), with crystallization epitope mutations a21y:a22t:t23p:r25l
49% identity, 98% coverage: 6:339/340 of query aligns to 7:337/344 of 6gqdA

query
sites
6gqdA

N

N

E

D

Y

H

P

Q

H

H

R

I

R

R

Y

Y

N

N

A

P

L

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T

Q

G

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E

E

W

W

V

V

L

L

V

V

S

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A

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M

K

K

R

R

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P

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W

Q

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G

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L

K

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H

-

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-

A

-

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-

V

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Y

H

D

D

P

P

G

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C
x
N

Y
x
P

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L

C
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C

P

P

G

G

N

A

S

I

R

R

S
x
A

G

N

G

G

E

E

Q

V

N

N

P

P

D

Q

Y

Y

K

D

D

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Y

F

V

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F

F

I

D

N
|
N

D
|
D

F

F

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A

A

L

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P

D

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A

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H

P

P

D

W

H
x
S

S

D

L

V

T

T

I

L

P

P

Q

L

M

M

K

S

V

V

E

P

D

E

I

I

R

R

K

A

V

V

D

D

L

A

W

W

I

A

E

S

E

V

Y

T

H

E

T

E

L

L

G

G

S

A

D

-

P

-

E

Q

I

Y

N

P

Y

W

V

V

Q

Q

I

I

F

F

E

E

N
|
N

K

K

G

G

E

A

I

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

S

S

Q

S

K

F

L

L

P

P

V

D

E

I

P

A

S

Q

K

R

K
x
E

Q

E

H

R

Y

S

Q

Q

L

Q

E

A

Y

Y

F

K

Q

S

K

Q

H

H

G

G

D

E

T

P

L

L

S

M

D

E

Y

Y

L

S

Q

R

K

Q

E

E

L

L

Q

L

K

R

D

K

E

E

R

R

I

L

I

V

F

L

E

T

N

S

N

E

H

H

F

W

A

L

A

V

I

L

V

V

P

P

F

F

W

W

A

A

T

T

W

W

P

P

Y

Y

E

Q

A

T

M

L

I

L

P

P

K

R

R

R

A

H

M

V

A

R

R

R

I

L

T

P

D

E

M

L

T

T

D

P

D

A

E

E

K

R

T

D

A

D

L

L

A

A

D

S

T

I

Y

M

K

K

R

K

L

L

T

L

M

T

I

K

Y

Y

D

D

K

N

V

L

F

F

D

E

V

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

T

D

G

G

S

K

E

E

A

H

G

N

A

E

N

W

W

-

D

H

H

W

W

-

Q

-

L

H
|
H

M

A

A
x
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

T

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

L

active site: N48 (≠ C51), C51 (= C54), S111 (≠ H114), N149 (= N154), S157 (= S162), H160 (= H165), H162 (= H167), Q164 (= Q169)
binding 5,6-dihydrouridine-5'-monophosphate: P49 (≠ Y52), A57 (≠ S60), N73 (= N76), D74 (= D77), H162 (= H167), Q164 (= Q169)
binding zinc ion: E178 (≠ K183), H277 (= H282), H295 (= H297), H297 (≠ A299)

5in3A Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase (see paper)
47% identity, 97% coverage: 10:339/340 of query aligns to 4:319/324 of 5in3A

query
sites
5in3A

H

H

R

I

R

R

Y

Y

N

N

A

P

L

L

T

Q

G

D

E

E

W

W

V

V

L

L

V

V

S

S

P

A

H

H

R

R

S

T

K

V

R

-

P

-

W

-

Q

-

G

-

Q

-

V

-

E

-

K

-

H

-

S

-

A

-

E

-

V

-

R

-

P

P

Q

R

Y

H

D

D

P

P

G

L

C
x
N

Y
x
P

L

L

C
|
C

P

P

G

G

N

A

S

I

R

R

S
x
A

G

N

G

G

E

E

Q

V

N

N

P

P

D

Q

Y

Y

K

D

D

S

V

T

Y

F

V

L

F
|
F

I

D

N
|
N

D
|
D

F

F

S

P

A

A

L

L

L

Q

K

P

D

D

S

A

P

P

D

S

F

P

D

G

V

P

V

S

E

D

D

H

D

P

I

L

F

F

K

Q

A

A

E

K

S

S

E

A

K

R

G

G

I

V

C

C

R

K

V
|
V

I

M

C

C

F

F

S

H

P

P

D

W

H
x
S

S

D

L

V

T

T

I

L

P

P

Q

L

M

M

K

S

V

V

E

P

D

E

I

I

R

R

K

A

V

V

D

D

L

A

W

W

I

A

E

S

E

V

Y

T

H

E

T

E

L

L

G

G

S

A

D

-

P

-

E

Q

I

Y

N

P

Y

W

V

V

Q

Q

I

I

F
|
F

E

E

N
|
N

K

K

G

G

E

A

I

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

A

A

S

S

Q

S

K

F

L

L

P

P

V

D

E

I

P

A

S

Q

K

R

K
x
E

Q

E

H

R

Y

S

Q

Q

L

Q

E

A

Y

Y

F

K

Q

S

K

Q

H

H

G

G

D

E

T

P

L

L

S

M

D

E

Y

Y

L

S

Q

R

K

Q

E

E

L

L

Q

L

K

R

D

K

E

E

R

R

I

L

I

V

F

L

E

T

N

S

N

E

H

H

F

W

A

L

A

V

I

L

V

V

P

P

F

F

W

W

A

A

T

T

W

W

P

P

Y

Y

E

Q

A

T

M

L

I

L

P

P

K

R

R

R

A

H

M

V

A

R

R

R

I

L

T

P

D

E

M

L

T

T

D

P

D

A

E

E

K

R

T

D

A

D

L

L

A

A

D

S

T

I

Y

M

K

K

R

K

L

L

T

L

M

T

I

K

Y

Y

D

D

K

N

V

L

F

F

D

E

V

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

I

W

H
|
H

Q

G

A

A

P

P

T

T

D

G

-

S

-

E

-

A

G

G

K

A

E

N

H

W

N

N

E

H

W

W

H

Q

W

L

H
|
H

M

A

A
x
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

T

T

V

V

K

R

K

K

F

F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

T

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

S

Q

A

A

Q

E

I

R

L

L

K

R

N

A

L

L

active site: N30 (≠ C51), C33 (= C54), S93 (≠ H114), N131 (= N154), S139 (= S162), H142 (= H165), H144 (= H167), Q146 (= Q169)
binding 1-O-phosphono-alpha-D-glucopyranose: F129 (= F152), N131 (= N154), Q146 (= Q169), V295 (= V315), G296 (= G316), E298 (= E318)
binding 5,6-dihydrouridine-5'-monophosphate: P31 (≠ Y52), A39 (≠ S60), F53 (= F74), N55 (= N76), D56 (= D77), V86 (= V107), H144 (= H167), Q146 (= Q169)
binding zinc ion: E160 (≠ K183), H259 (= H282), H277 (= H297), H279 (≠ A299)

6k5zB Structure of uridylyltransferase (see paper)
29% identity, 96% coverage: 12:337/340 of query aligns to 4:306/314 of 6k5zB

query
sites
6k5zB

R

R

Y

K

N

D

A

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

V

V

S

S

P

P

H

H

R

R

S

L

K

K

R

R

P

P

W

W

Q

Q

G

P

Q

-

V

-

E

-

K

-

H

-

S

-

A

-

E

-

V

-

R

-

P

-

Q

-

Y

-

D

E

P

G

G

A

C
|
C

Y

P

L

F

C
|
C

P

P

G

G

N

A

S

P

R

E

S

T

G

G

G

R

E

G

Q

W

N

-

P

-

D

-

Y

-

K

-

D

D

V

V

Y

L

V

I

F

L

I

P

N

N

D

R

F

Y

S

P

A

V

L

V

L

T

K

E

D

N

S

P

P

P

D

E

F

-

D

-

V

P

V

T

E

A

D

E

D

D

I

L

F

Y

K

E

A

V

E

I

S

P

E

A

K

R

G

G

I

S

C

S

R

L

V

V

I

V

C

V

F

E

S

T

P

P

D

Q

H
|
H

S

D

L

V

T

D

-

D

I

L

P

S

Q

D

M

L

K

P

V

L

E

G

D

Q

I

I

R

K

K

K

V

I

V

L

D

T

L

A

W

V

I

A

E

E

E

A

Y

Q

H

R

T

K

L

A

G

E

S

K

D

E

P

G

E

N

I

A

N

A

Y

Y

V

F

Q

L

I

F

F

F

E

R

N
|
N

K

K

G

G

E

K

I

E

M

I

G

G

C
x
V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
|
H

G

S

Q
|
Q

I

I

W

Y

A

I

S

L

Q

P

K

V

L

V

P

P

V

P

E

R

P

V

S

R

K

A

K
x
E

Q

L

H

Q

Y

A

Q

S

L

Y

E

E

Y

W

F

Y

Q

V

K

K

H

H

G

G

D

S

T
x
C

L

L

L

H

S
x
C

D

R

Y

I

L

V

Q

E

K

K

E

E

L

-

Q

-

K

-

D

E

E

K

R

R

I

L

I

V

F

F

E

Q

N

N

N

R

H

N

F

W

A

K

A

A

I

F

V

V

P

P

F

F

W

Y

A

A

T

K

W

W

P

P

Y

H

E

E

A

V

M

H

I

I

I

Y

P

P

K

K

R

R

A
x
H

M

R

A

S

R

L

I

L

T

T

D

E

M

L

T

T

D

D

D

E

E

E

K

V

T

A

A

D

L

L

A

A

D

E

T

A

Y

L

K

K

R

I

L

T

T

L

M

C

I

A

Y

L

D

K

K

Q

V

V

F

A

D

G

V

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

I

L

H
|
H

Q

Q

A

A

P

P

T

L

D

P

G

-

K

R

E

P

H

T

N

Q

E

Y

W

Y

H
|
H

W

L

H
|
H

M

F

A
x
E

F

I

Y

Y

P

-

P

G

L

M

L

Y

R

R

S

P

A

D

T

G

V

K

K

L

K

K

F

H

M

A

V

A

G

G

Y

A

E

E

M

L

L

G

A

A

T

S

P

L

-

F

Q

T

R

L

D

D

I

T

T

T

P

P

E

E

S

E

A

T

A

A

Q

A

I

R

L

I

K

K

active site: C30 (= C51), C33 (= C54), H86 (= H114), N127 (= N154), S135 (= S162), H138 (= H165), H140 (= H167), Q142 (= Q169)
binding fe (iii) ion: E156 (≠ K183), H252 (= H282), H266 (= H297), E268 (≠ A299)
binding phosphate ion: V134 (≠ C161), S135 (= S162), L136 (≠ N163), H140 (= H167)
binding zinc ion: C30 (= C51), C33 (= C54), H86 (= H114), H138 (= H165), C170 (≠ T197), C173 (≠ S200), H211 (≠ A241), H264 (= H295)

6k9zA Structure of uridylyltransferase mutant (see paper)
27% identity, 96% coverage: 12:337/340 of query aligns to 4:299/309 of 6k9zA

query
sites
6k9zA

R

R

Y

K

N

D

A

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

V

V

S

S

P

P

H

-

R

-

S

-

K

-

R

-

P

-

W

-

Q

-

G

-

Q

-

V

-

E

-

K

-

H

-

S

-

A

-

E

-

V

-

R

-

P

-

Q

Q

Y

P

D

E

P

G

G

A

C
|
C

Y
x
P

L

F

C
|
C

P

P

G

G

N

A

S

P

R

E

S

T

G

G

G

R

E

G

Q

W

N

-

P

-

D

-

Y

-

K

-

D

D

V

V

Y

L

V

I

F

L

I

P

N
|
N

D
x
R

F
x
Y

S

P

A

V

L

V

L

T

K

E

D

N

S

P

P

P

D

E

F

-

D

-

V

P

V

T

E

A

D

E

D

D

I

L

F

Y

K

E

A

V

E

I

S

P

E

A

K

R

G

G

I

S

C

S

R

L

V

V

I

V

C

V

F

E

S

T

P

P

D

Q

H
|
H

S

D

L

V

T

D

-

D

I

L

P

S

Q

D

M

L

K

P

V

L

E

G

D

Q

I

I

R

K

K

K

V

I

V

L

D

T

L

A

W

V

I

A

E

E

E

A

Y

Q

H

R

T

K

L

A

G

E

S

K

D

E

P

G

E

N

I

A

N

A

Y

Y

V

F

Q

L

I

F

F

F

E

R

N
|
N

K

K

G

G

E

K

I

E

M

I

G

G

C

V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
x
F

G

S

Q
|
Q

I

I

W
x
Y

A

I

S

L

Q

P

K

V

L

V

P

P

V

P

E

R

P

V

S

R

K

A

K
x
E

Q

L

H

Q

Y

A

Q

S

L

Y

E

E

Y

W

F

Y

Q

V

K

K

H

H

G

G

D

S

T
x
C

L

L

L

H

S
x
C

D

R

Y

I

L

V

Q

E

K

K

E

E

L

-

Q

-

K

-

D

E

E

K

R

R

I

L

I

V

F

F

E

Q

N

N

N

R

H

N

F

W

A

K

A

A

I

F

V

V

P

P

F

F

W

Y

A

A

T

K

W

W

P

P

Y

H

E

E

A

V

M

H

I

I

I

Y

P

P

K

K

R

R

A
x
H

M

R

A

S

R

L

I

L

T

T

D

E

M

L

T

T

D

D

D

E

E

E

K

V

T

A

A

D

L

L

A

A

D

E

T

A

Y

L

K

K

R

I

L

T

T

L

M

C

I

A

Y

L

D

K

K

Q

V

V

F

A

D

G

V

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

I

L

H
|
H

Q

Q

A

A

P

P

T

L

D

P

G

-

K

R

E

P

H

T

N

Q

E

Y

W

Y

H
|
H

W

L

H
|
H

M

F

A
x
E

F

I

Y

Y

P

-

P

G

L

M

L

Y

R

R

S

P

A

D

T

G

V

K

K

L

K

K

F

H

M

A

V

A

G

G

Y

A

E

E

M

L

L

G

A

A

T

S

P

L

-

F

Q

T

R

L

D

D

I

T

T

T

P

P

E

E

S

E

A

T

A

A

Q

A

I

R

L

I

K

K

active site: C23 (= C51), C26 (= C54), H79 (= H114), N120 (= N154), S128 (= S162), H131 (= H165), F133 (≠ H167), Q135 (= Q169)
binding fe (iii) ion: E149 (≠ K183), H245 (= H282), H259 (= H297), E261 (≠ A299)
binding uridine-5'-diphosphate: P24 (≠ Y52), N43 (= N76), R44 (≠ D77), Y45 (≠ F78), L129 (≠ N163), Q135 (= Q169), Y137 (≠ W171)
binding zinc ion: C23 (= C51), C26 (= C54), H79 (= H114), H131 (= H165), C163 (≠ T197), C166 (≠ S200), H204 (≠ A241), H257 (= H295)

Q9FK51 ADP-glucose phosphorylase; ADP-glucose:phosphate adenylyltransferase; EC 2.7.7.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
25% identity, 99% coverage: 6:340/340 of query aligns to 20:349/351 of Q9FK51

query
sites
Q9FK51

N

N

E

Q

Y

S

P

P

H

E

R

L

R

R

Y

K

N

D

A

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

A

R
|
R

S
x
A

K
|
K

R
|
R

P

P

W

T

Q

D

G

F

Q

-

V

-

E

-

K

K

H

S

S

K

A

S

E

P

V

Q

R

N

P

P

Q

N

Y

P

D

K

P

P

G

S

-

S

C
|
C

Y

P

L

F

C
|
C

P

I

G

G

N

R

S

E

R

Q

S
x
E

G
x
C

G
x
A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

E

D

Q

H

N

D

P

P

D

N

Y

W

K

K

D

-

V

L

Y

R

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F

I

I

E

N
|
N

D

L

F

Y

S

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A

A

L

L

L

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R

D

N

S

L

P

-

D

-

F

-

D

-

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-

E

-

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-

D

E

I

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F

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K

S

A

T

E

Q

S

P

E

E

K

T

G

G

I

T

C

S

R

R

-

T

-

I

-

V

-

G

-

F

-

G

-

F

-

H

-

D

V

V

I

V

C

I

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H
|
H

S

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I

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D

M

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D

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I

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N

T

Q

L

I

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A

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H

P

D

E

S

I

I

N

N

Y

Y

V

I

Q

Q

I

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F

F

E

K

N
|
N

K

Q

G

G

E

A

I

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M

A

G
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G

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x
A

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S

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x
M

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|
H

P

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H
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H

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Q

I

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M

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A

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V

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P

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T

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Y

Y

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K

E

H

T

G

G

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x
C

L

C

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x
C

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E

Y

A

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S

K

K

E

H

L

F

Q

-

K

-

D

-

E

-

R

-

I

V

I

I

F

D

E

E

N

S

H

H

F

F

A

V

A

S

I

V

V

A

P

P

F

F

W

A

A

A

T

T

W

Y

P

P

Y

F

E

E

A

I

M

W

I

I

P

P

K

K

R

D

A
x
H

M

S

A

S

R

H

I

F

T

H

D

H

M

L

T

D

D

D

D

V

E

K

K

A

T

V

A

D

L

L

A

G

D

G

T

L

Y

L

K

K

R

L

L

M

T

L

M

Q

I

K

Y

I

D

A

K

K

V

Q

F

L

D

N

V

-

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

Q

T

A

S

P

P

T

L

D

K

G

V

K

T

E

E

H

S

N

Q

E

L

-

P

-

Y

W

T

H
|
H

W

W

H

F

M

L

A

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

T
x
G

V

V

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

T

N

P

P

Q

-

R

-

D

-

I

V

T

F

P

P

E

E

S

D

A

V

A

A

Q

K

I

V

L

M

K

R

N

E

L

V

N

S

RAKR 41:44 (≠ RSKR 27:30) binding ADP-alpha-D-glucose
C63 (= C51) binding Zn(2+)
C66 (= C54) binding Zn(2+)
ECA 72:74 (≠ SGG 60:62) binding ADP-alpha-D-glucose
N94 (= N76) binding ADP-alpha-D-glucose
H133 (= H114) binding Zn(2+)
N173 (= N154) binding ADP-alpha-D-glucose
GASM 179:182 (≠ GCSN 160:163) binding ADP-alpha-D-glucose
H184 (= H165) binding Zn(2+)
H186 (= H167) active site, Tele-AMP-histidine intermediate
Q188 (= Q169) binding ADP-alpha-D-glucose
C216 (≠ T197) binding Zn(2+)
C219 (≠ S200) binding Zn(2+)
H255 (≠ A241) binding Zn(2+)
H310 (= H295) binding Zn(2+)
G321 (≠ T309) binding ADP-alpha-D-glucose
FE 325:326 (≠ FM 313:314) binding ADP-alpha-D-glucose

2h39B Crystal structure of an adp-glucose phosphorylase from arabidopsis thaliana with bound adp-glucose
24% identity, 98% coverage: 7:340/340 of query aligns to 1:311/313 of 2h39B

query
sites
2h39B

E

Q

Y

S

P

P

H

E

R

L

R

R

Y

K

N

D

A

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

A

R
|
R

S

A

K

K

R
|
R

P

P

W

T

Q

D

G

-

Q

-

V

-

E

-

K

-

H

-

S

-

A

-

E

-

V

-

R

-

P

-

Q

-

Y

F

D

P

P

S

G

S

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

S

E

R

Q

S

E

G
x
C

G

A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

E

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H

N

D

P

P

D

N

Y

W

K

K

D

-

V

L

Y

R

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V

F

I

I

E

N
|
N

D
x
L

F
x
Y

S

P

A

A

L

L

L

S

K

R

D

N

S

-

P

-

D

-

F

-

D

-

V

-

V

L

E

E

D

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D

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I

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A

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E

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K

F

G

G

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|
H

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M

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G

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D

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Y

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H

N

T

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L

I

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E

S

I

I

N

N

Y

Y

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F
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F

E

K

N
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N

K

Q

G

G

E

A

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M

A

G
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G

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x
A

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S

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x
M

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H

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H
x
G

G

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Q

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M

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M

A

A

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D

Y

Y

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F

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E

H

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G

G

D

K

T
x
C

L

C

L

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S
x
C

D

E

Y

A

L

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Q

S

K

K

E

H

L

F

Q

-

K

-

D

-

E

-

R

-

I

V

I

I

F

D

E

E

N

S

H

H

F

F

A

V

A

S

I

V

V

A

P

P

F

F

W

A

A

A

T

T

W

Y

P

P

Y

F

E

E

A

I

M

W

I

I

P

P

K

K

R

D

A
x
H

M

S

A

S

R

H

I

F

T

H

D

H

M

L

T

D

D

D

D

V

E

K

K

A

T

V

A

D

L

L

A

G

D

G

T

L

Y

L

K

K

R

L

L

M

T

L

M

Q

I

K

Y

I

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A

K

K

V

Q

F

L

D

N

V

-

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

Q

T

A

S

P

P

T

L

D

K

G

V

K

T

E

E

H

S

N

Q

E

L

-

P

-

Y

W

T

H
|
H

W

W

H

F

M

L

A

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

T

G

V

V

K
x
G

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

T

N

P

P

Q

-

R

-

D

-

I

V

T

F

P

P

E

E

S

D

A

V

A

A

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K

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L

M

K

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N

E

L

V

N

S

active site: C32 (= C51), C35 (= C54), H95 (= H114), N135 (= N154), S143 (= S162), H146 (= H165), G148 (≠ H167), Q150 (= Q169)
binding adenosine-5'-diphosphate-glucose: R21 (= R27), R24 (= R30), F34 (≠ L53), C42 (≠ G61), N63 (= N76), L64 (≠ D77), Y65 (≠ F78), F133 (= F152), N135 (= N154), G141 (= G160), A142 (≠ C161), S143 (= S162), M144 (≠ N163), Q150 (= Q169), G285 (≠ K311), F287 (= F313), E288 (≠ M314)
binding zinc ion: C32 (= C51), H95 (= H114), H146 (= H165), C178 (≠ T197), C181 (≠ S200), H217 (≠ A241), H272 (= H295)

1z84A X-ray structure of galt-like protein from arabidopsis thaliana at5g18200 (see paper)
24% identity, 98% coverage: 9:340/340 of query aligns to 2:309/311 of 1z84A

query
sites
1z84A

P

P

H

E

R

L

R

R

Y

K

N

D

A

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

-

R

-

S

-

K

-

R

R

P

P

W

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Q

-

G

-

Q

-

V

-

E

-

K

-

H

-

S

-

A

T

E

D

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F

R

K

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S

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K

Y

S

D

P

P

S

G

S

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

S

E

R

Q

S

E

G

C

G

A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

E

D

Q

H

N

D

P

P

D

N

Y

W

K

K

D

-

V

L

Y

R

V

V

F

I

I

E

N
|
N

D
x
L

F
x
Y

S

P

A

A

L

L

L

-

K

-

D

-

S

S

P

R

D

N

F

L

D

E

V

T

V

Q

E

S

D

R

D

T

I

I

F

V

K

G

A

F

E

-

S

-

E

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K

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G

G

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F

C

H

R

D

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V

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H
|
H

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S

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I

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D

M

I

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D

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P

E

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G

I

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G

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L

D

I

L

A

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Y

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K

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Y

I

H

N

T

Q

L

I

G

A

S

Q

D

H

P

D

E

S

I

I

N

N

Y

Y

V

I

Q

Q

I

V

F

F

E

K

N
|
N

K

Q

G

G

E

A

I

S

M

A

G

G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
|
H

G

S

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|
Q

I

M

W

M

A

A

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L

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P

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P

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S

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Y

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L

K

E

D

Y

Y

F

F

Q

E

K

E

H

T

G

G

D

K

T
x
C

L

C

L

L

S
x
C

D

E

Y

A

L

K

Q

S

K

K

E

H

L

F

Q

-

K

-

D

-

E

-

R

-

I

V

I

I

F

D

E

E

N

S

H

H

F

F

A

V

A

S

I

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V

A

P

P

F

F

W

A

A

A

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T

W

Y

P

P

Y

F

E

E

A

I

M

W

I

I

P

P

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K

R

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A
x
H

M

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A

S

R

H

I

F

T

H

D

H

M

L

T

D

D

D

D

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E

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A

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A

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L

A

G

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L

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L

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L

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M

T

L

M

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I

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Y

I

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A

K

K

V

Q

F

L

D

N

V

-

S

D

F

P

P

P

Y

Y

S

N

A

Y

G

M

I

I

H

H

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T

A

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P

T

L

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K

G

V

K

T

E

E

H

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N

Q

E

L

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P

-

Y

W

T

H
|
H

W

W

H

F

M

L

A

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

T

G

V

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K

G

F

F

M

E

V

I

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G

Y

T

E

G

M

C

L

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A

I

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P

P

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R

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D

-

I

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T

F

P

P

E

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N

E

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active site: C31 (= C51), C34 (= C54), H93 (= H114), N133 (= N154), S141 (= S162), H144 (= H165), H146 (= H167), Q148 (= Q169)
binding adenosine monophosphate: F33 (≠ L53), N62 (= N76), L63 (≠ D77), Y64 (≠ F78), N133 (= N154), A140 (≠ C161), S141 (= S162), M142 (≠ N163), H146 (= H167), Q148 (= Q169)
binding zinc ion: C31 (= C51), C34 (= C54), H93 (= H114), H144 (= H165), C176 (≠ T197), C179 (≠ S200), H215 (≠ A241), H270 (= H295)

Query Sequence

>WP_035135395.1 NCBI__GCF_000769915.1:WP_035135395.1
MDKNLNEYPHRRYNALTGEWVLVSPHRSKRPWQGQVEKHSAEVRPQYDPGCYLCPGNSRS
GGEQNPDYKDVYVFINDFSALLKDSPDFDVVEDDIFKAESEKGICRVICFSPDHSLTIPQ
MKVEDIRKVVDLWIEEYHTLGSDPEINYVQIFENKGEIMGCSNPHPHGQIWASQKLPVEP
SKKQHYQLEYFQKHGDTLLSDYLQKELQKDERIIFENNHFAAIVPFWATWPYEAMIIPKR
AMARITDMTDDEKTALADTYKRLTMIYDKVFDVSFPYSAGIHQAPTDGKEHNEWHWHMAF
YPPLLRSATVKKFMVGYEMLATPQRDITPESAAQILKNLN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory