SitesBLAST
Comparing WP_035218082.1 NCBI__GCF_000429905.1:WP_035218082.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
42% identity, 97% coverage: 7:257/258 of query aligns to 1:251/252 of 1vl8B
- active site: G17 (= G23), S143 (= S148), I154 (≠ A161), Y157 (= Y163), K161 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G19), R16 (≠ S22), G17 (= G23), L18 (= L24), S37 (≠ A43), R38 (= R44), C63 (= C68), D64 (= D69), V65 (= V70), A91 (≠ N96), A92 (= A97), G93 (= G98), I94 (≠ V99), V114 (≠ I119), I141 (= I146), S143 (= S148), Y157 (= Y163), K161 (= K167), P187 (= P193), G188 (= G194), Y190 (≠ F196), T192 (≠ S198), M194 (= M200), T195 (= T201)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
40% identity, 95% coverage: 10:254/258 of query aligns to 3:244/247 of 4jroC
- active site: G16 (= G23), S142 (= S148), Q152 (= Q160), Y155 (= Y163), K159 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G19), S14 (= S21), R15 (≠ S22), G16 (= G23), I17 (≠ L24), N35 (≠ A42), Y36 (≠ A43), N37 (≠ R44), G38 (≠ R45), S39 (≠ T46), N63 (≠ D69), V64 (= V70), N90 (= N96), A91 (= A97), I93 (≠ V99), I113 (= I119), S142 (= S148), Y155 (= Y163), K159 (= K167), P185 (= P193), I188 (≠ F196), T190 (≠ S198)
3uf0A Crystal structure of a putative NAD(p) dependent gluconate 5- dehydrogenase from beutenbergia cavernae(efi target efi-502044) with bound NADP (low occupancy)
41% identity, 97% coverage: 8:257/258 of query aligns to 3:248/249 of 3uf0A
- active site: G18 (= G23), S141 (= S148), V151 (≠ I158), Y154 (= Y163), K158 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G19), S17 (= S22), G18 (= G23), I19 (≠ L24), R39 (= R45), D63 (= D69), L64 (≠ V70), N89 (= N96), G91 (= G98), I92 (≠ V99), I139 (= I146), A140 (= A147), S141 (= S148), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), V187 (≠ F196), T189 (≠ S198), N191 (≠ M200), T192 (= T201)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
38% identity, 95% coverage: 10:254/258 of query aligns to 4:243/247 of P73574
- A14 (= A20) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ I158) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K167) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ W195) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ R209) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
4za2D Crystal structure of pectobacterium carotovorum 2-keto-3-deoxy-d- gluconate dehydrogenase complexed with NAD+ (see paper)
39% identity, 97% coverage: 8:257/258 of query aligns to 6:246/247 of 4za2D
- binding nicotinamide-adenine-dinucleotide: G17 (= G19), D19 (≠ S21), L22 (= L24), I42 (≠ T46), D65 (= D69), M66 (≠ V70), N92 (= N96), A93 (= A97), G94 (= G98), L115 (≠ I119), I143 (= I146), S145 (= S148), Y158 (= Y163), K162 (= K167), G189 (= G194), M191 (≠ F196), T193 (≠ S198), N195 (≠ M200)
5u9pB Crystal structure of a gluconate 5-dehydrogenase from burkholderia cenocepacia j2315 in complex with NADP and tartrate
33% identity, 100% coverage: 1:257/258 of query aligns to 5:259/261 of 5u9pB
- active site: G27 (= G23), S152 (= S148), Y165 (= Y163), K169 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G23 (= G19), R26 (≠ S22), G27 (= G23), I28 (≠ L24), R48 (= R44), D73 (= D69), V74 (= V70), N100 (= N96), A101 (= A97), I150 (= I146), Y165 (= Y163), K169 (= K167), P195 (= P193), F198 (= F196), T200 (≠ S198), L202 (≠ M200), N203 (≠ T201)
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
38% identity, 93% coverage: 16:254/258 of query aligns to 5:241/244 of 1edoA
- active site: G12 (= G23), S138 (= S148), Y151 (= Y163), K155 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G19), S10 (= S21), R11 (≠ S22), I13 (≠ L24), N31 (vs. gap), Y32 (≠ A42), A33 (= A43), R34 (= R44), S35 (≠ R45), D59 (= D69), V60 (= V70), N86 (= N96), A87 (= A97), S138 (= S148), Y151 (= Y163), K155 (= K167), P181 (= P193), G182 (= G194), I184 (≠ F196), S186 (= S198), M188 (= M200)
7vyqA Short chain dehydrogenase (scr) cryoem structure with NADP and ethyl 4-chloroacetoacetate (see paper)
34% identity, 99% coverage: 2:257/258 of query aligns to 25:279/280 of 7vyqA
- binding ethyl 4-chloranyl-3-oxidanylidene-butanoate: T123 (≠ A100), S173 (= S148), Q183 (= Q160), Y219 (≠ W195), F227 (= F205), W236 (≠ M214)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G42 (= G19), S44 (= S21), G45 (≠ S22), G46 (= G23), I47 (≠ L24), N67 (≠ R45), H69 (≠ D47), I93 (≠ V70), N119 (= N96), A120 (= A97), G121 (= G98), V144 (≠ I119), S173 (= S148), Y188 (= Y163), K192 (= K167), P217 (= P193), G218 (= G194), I220 (≠ F196), T222 (≠ S198), I224 (≠ M200), T225 (= T201)
7dlmA Short chain dehydrogenase (scr) crystal structure with NADPH (see paper)
34% identity, 99% coverage: 2:257/258 of query aligns to 25:279/280 of 7dlmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G42 (= G19), S44 (= S21), G45 (≠ S22), G46 (= G23), I47 (≠ L24), Y66 (≠ R44), N67 (≠ R45), S68 (≠ T46), H69 (≠ D47), C91 (= C68), N92 (≠ D69), I93 (≠ V70), N119 (= N96), A120 (= A97), G121 (= G98), V122 (= V99), T171 (≠ I146), S173 (= S148), Y188 (= Y163), K192 (= K167), P217 (= P193), G218 (= G194), I220 (≠ F196), T222 (≠ S198), I224 (≠ M200)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
37% identity, 94% coverage: 13:254/258 of query aligns to 5:243/246 of 3osuA
4dmmB 3-oxoacyl-[acyl-carrier-protein] reductase from synechococcus elongatus pcc 7942 in complex with NADP
39% identity, 95% coverage: 10:254/258 of query aligns to 3:236/240 of 4dmmB
- active site: G16 (= G23), S142 (= S148), Q152 (= Q160), Y155 (= Y163), K159 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G19), S14 (= S21), R15 (≠ S22), G16 (= G23), I17 (≠ L24), A37 (= A43), S38 (≠ R44), S39 (≠ R45), A62 (≠ C68), D63 (= D69), V64 (= V70), N90 (= N96), A91 (= A97), L113 (≠ I119), I140 (= I146), S142 (= S148), Y155 (= Y163), K159 (= K167), P185 (= P193), G186 (= G194), I188 (≠ F196), T190 (≠ S198), M192 (= M200)
Q9BY49 Peroxisomal trans-2-enoyl-CoA reductase; TERP; 2,4-dienoyl-CoA reductase-related protein; DCR-RP; HPDHase; Short chain dehydrogenase/reductase family 29C member 1; pVI-ARL; EC 1.3.1.38 from Homo sapiens (Human) (see paper)
33% identity, 95% coverage: 10:254/258 of query aligns to 16:264/303 of Q9BY49
Sites not aligning to the query:
- 303 mutation Missing: Abolishes localization to peroxisomes.
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
38% identity, 96% coverage: 10:257/258 of query aligns to 8:252/258 of 4wecA
- active site: G21 (= G23), S143 (= S148), Q154 (= Q160), Y157 (= Y163), K161 (= K167)
- binding nicotinamide-adenine-dinucleotide: G17 (= G19), A19 (≠ S21), S20 (= S22), G21 (= G23), I22 (≠ L24), D41 (≠ A43), I42 (≠ R44), V61 (= V66), D62 (= D69), V63 (= V70), N89 (= N96), T141 (≠ I146), Y157 (= Y163), K161 (= K167), P187 (= P193), P189 (≠ W195), V190 (≠ F196)
4hp8B Crystal structure of a putative 2-deoxy-d-gluconate 3-dehydrogenase from agrobacterium tumefaciens (target efi-506435) with bound NADP
39% identity, 99% coverage: 2:257/258 of query aligns to 1:245/246 of 4hp8B
- active site: G19 (= G23), S138 (= S148), V148 (≠ I158), Y151 (= Y163), K155 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G19), N17 (≠ S21), T18 (≠ S22), G19 (= G23), L20 (= L24), R40 (= R44), R41 (= R45), D63 (= D69), F64 (≠ V70), N85 (= N96), G87 (= G98), I88 (≠ V99), I136 (= I146), Y151 (= Y163), K155 (= K167), P181 (= P193), G182 (= G194), I184 (≠ F196), T186 (≠ S198), N188 (≠ M200), T189 (= T201)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
35% identity, 97% coverage: 7:257/258 of query aligns to 2:254/255 of 5itvA
- active site: G18 (= G23), S141 (= S148), Y154 (= Y163), K158 (= K167)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G19), S17 (= S22), G18 (= G23), I19 (≠ L24), D38 (≠ R45), I39 (≠ T46), T61 (≠ C68), I63 (≠ V70), N89 (= N96), G91 (= G98), T139 (≠ I146), S141 (= S148), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), I186 (≠ W195), I187 (≠ F196)
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
37% identity, 94% coverage: 13:254/258 of query aligns to 2:236/239 of 3sj7A
- active site: G12 (= G23), S138 (= S148), Q148 (= Q160), Y151 (= Y163), K155 (= K167)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G19), S10 (= S21), R11 (≠ S22), I13 (≠ L24), N31 (≠ L41), Y32 (≠ A42), A33 (= A43), G34 (≠ R44), S35 (≠ R45), A58 (≠ C68), N59 (≠ D69), V60 (= V70), N86 (= N96), A87 (= A97), T109 (≠ I119), S138 (= S148), Y151 (= Y163), K155 (= K167), P181 (= P193), G182 (= G194)
3lqfA Crystal structure of the short-chain dehydrogenase galactitol- dehydrogenase (gatdh) of rhodobacter sphaeroides in complex with NAD and erythritol (see paper)
39% identity, 97% coverage: 7:256/258 of query aligns to 6:252/254 of 3lqfA
- active site: G22 (= G23), S144 (= S148), Y159 (= Y163), K163 (= K167)
- binding meso-erythritol: N151 (≠ S157), Y159 (= Y163), Y191 (≠ W195), T197 (= T201), M200 (= M204)
- binding nicotinamide-adenine-dinucleotide: G18 (= G19), S21 (= S22), G22 (= G23), I23 (≠ L24), D42 (≠ A43), R43 (= R44), D66 (= D69), V67 (= V70), S92 (≠ N96), L142 (≠ I146), S144 (= S148), K163 (= K167), P189 (= P193), V192 (≠ F196), T194 (≠ S198), M196 (= M200), T197 (= T201)
2wsbA Crystal structure of the short-chain dehydrogenase galactitol- dehydrogenase (gatdh) of rhodobacter sphaeroides in complex with NAD (see paper)
39% identity, 97% coverage: 7:256/258 of query aligns to 6:252/254 of 2wsbA
- active site: G22 (= G23), S144 (= S148), Y159 (= Y163), K163 (= K167)
- binding nicotinamide-adenine-dinucleotide: G18 (= G19), S21 (= S22), G22 (= G23), I23 (≠ L24), D42 (≠ A43), R43 (= R44), D66 (= D69), V67 (= V70), S92 (≠ N96), A93 (= A97), L142 (≠ I146), S144 (= S148), Y159 (= Y163), K163 (= K167), P189 (= P193), V192 (≠ F196), T194 (≠ S198), M196 (= M200), T197 (= T201)
- binding n-propanol: S144 (= S148), M145 (= M149), N151 (≠ S157), N151 (≠ S157), Y159 (= Y163), Y159 (= Y163), Y191 (≠ W195)
2wdzA Crystal structure of the short chain dehydrogenase galactitol- dehydrogenase (gatdh) of rhodobacter sphaeroides in complex with NAD+ and 1,2-pentandiol (see paper)
39% identity, 97% coverage: 7:256/258 of query aligns to 6:252/254 of 2wdzA
- active site: G22 (= G23), S144 (= S148), Y159 (= Y163), K163 (= K167)
- binding (2S)-pentane-1,2-diol: A45 (≠ T46), D49 (= D51), R62 (≠ E64), S146 (≠ L150), Y159 (= Y163)
- binding nicotinamide-adenine-dinucleotide: G18 (= G19), S21 (= S22), G22 (= G23), I23 (≠ L24), D42 (≠ A43), R43 (= R44), A65 (≠ C68), D66 (= D69), V67 (= V70), S92 (≠ N96), A93 (= A97), L142 (≠ I146), S144 (= S148), Y159 (= Y163), K163 (= K167), P189 (= P193), V192 (≠ F196), T194 (≠ S198), M196 (= M200), T197 (= T201)
C0KTJ6 Galactitol 2-dehydrogenase (L-tagatose-forming); Galactitol dehydrogenase; GDH; GatDH; Galactitol:NAD(+) 5-oxidoreductase; EC 1.1.1.406 from Cereibacter sphaeroides (Rhodobacter sphaeroides) (see paper)
39% identity, 97% coverage: 7:256/258 of query aligns to 6:252/254 of C0KTJ6
- SGI 21:23 (≠ SGL 22:24) binding NAD(+)
- D42 (≠ A43) binding NAD(+)
- DV 66:67 (= DV 69:70) binding NAD(+)
- Y159 (= Y163) binding NAD(+)
- K163 (= K167) binding NAD(+)
- VAT 192:194 (≠ FPS 196:198) binding NAD(+)
Sites not aligning to the query:
Query Sequence
>WP_035218082.1 NCBI__GCF_000429905.1:WP_035218082.1
MKSPAELFDLTGKTAVITGASSGLGVAFAQGLAAAGANVVLAARRTDRLKDLAANLEKTG
VGAEPVTCDVTLENDVDNMVKATTDRFGRLDILVNNAGVAIPHPAETEPYESFKMVMDIN
VNAQFLCSQRCGRIMLEARSGSIINIASMLGLVGLGSIPQASYNASKAAMINMTRELAAQ
WSKRGVRVNAIAPGWFPSEMTTDMFGDERSEAFMEKRSLLRRGGRTEELIGALLLLASEA
GSYITGQTIVVDGGWTAI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory