SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035218963.1 NCBI__GCF_000429905.1:WP_035218963.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3i5tA Crystal structure of aminotransferase prk07036 from rhodobacter sphaeroides kd131
45% identity, 96% coverage: 8:437/447 of query aligns to 7:434/444 of 3i5tA

query
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3i5tA

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active site: A14 (vs. gap), Y144 (= Y144), E216 (= E217), D249 (= D250), V252 (= V253), K279 (= K280), V411 (≠ I414)
binding pyridoxal-5'-phosphate: G111 (= G111), S112 (= S112), Y144 (= Y144), H145 (= H145), E216 (= E217), D249 (= D250), V251 (= V252), K279 (= K280), Y315 (≠ F317), T316 (= T318)

5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
38% identity, 99% coverage: 2:444/447 of query aligns to 8:460/460 of 5kr6B

query
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binding pyridoxal-5'-phosphate: S121 (= S110), G122 (= G111), S123 (= S112), Y155 (= Y144), H156 (= H145), G157 (= G146), E228 (= E217), D261 (= D250), V263 (= V252), K290 (= K280)

6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
38% identity, 98% coverage: 7:444/447 of query aligns to 10:449/450 of 6gwiB

query
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6gwiB

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L

E

E

C

L

V

V

V

A

S

D

D

K

N

T

E

T

-

G

-

Q

-

R

-

F

D

D

E

K

N

S

I

L

A

G

V

A

A

G

Q

N

R

L

V

C

D

R

E

D

F

L

C

C

Q

F

E

A

K

N

G

G

L

L

I

V

V

M

R

R

P

S

Y

V

E

G

N

D

L

T

C

M

I

I

L

I

S

S

P

P

P

P

L

L

I

V

I

I

E

R

K

R

K

E

H

E

A

I

D

D

Q

E

I

L

V

V

D

E

I

L

L

A

R

R

D

R

S

A

I

L

A

D

S

E

T

T

M

A

D

R

E

Q

M

L

active site: F18 (≠ W15), Y149 (= Y144), D255 (= D250), K284 (= K280)
binding pyridoxal-5'-phosphate: S115 (= S110), G116 (= G111), S117 (= S112), Y149 (= Y144), H150 (= H145), G151 (= G146), E222 (= E217), D255 (= D250), V257 (= V252), I258 (≠ V253), K284 (= K280)

5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
40% identity, 98% coverage: 7:442/447 of query aligns to 9:454/455 of 5kr5A

query
sites
5kr5A

D

D

K

R

K

A

H

H

I

F

I

M

H

H

P

P

W

S

S

T

N

H

L

A

G

Y

S

D

D

H

A

A

E

S

S

G

M

A

-

L

-

P

-

G

-

R

I

I

I

I

E

T

S

G

G

G

K

K

G

G

V

I

H

R

V

I

F

E

D

D

S

H

E

E

G

G

N

R

K

E

F

Y

L

I

D

D

S

A

I

F

S

A

G

G

M

L

W

Y

C

C

V

V

N

N

L

I

G

G

Y

Y

G

G

N

R

E
|
E

E

E

M

V

A

A

Q
x
D

A

A

I

I

A

Y

D

E

Q

Q

C

A

V

K

K

Q

L

L

A

A

Y

Y

Y

Y

T

H

P

T

F

Y

G

V

A

G

M

H

T

S

S

N

P

D

P

P

S

V

I

I

Q

E

L

L

A

S

T

S

E

R

L

I

-

I

S

E

A

D

L

W

T

A

P

P

G

A

D

G

L

M

N

K

C

K

F

V

Q

F

F

Y

T

G

N

M

S
|
S

G
|
G

S
|
S

T

D

A

A

V

N

E

E

S

T

A

Q

I

I

R

K

F

L

V

V

H

W

Y

Y

Y

Y

F

N

N

N

C

V

L

L

G

G

Q

R

P

P

E

N

K

K

K

K

H

K

I

I

I

I

Y

A

R

R

E

E

N

R

A

S

Y
|
Y

H
|
H

G
|
G

S

S

T

G

Y

I

L

V

A

T

A

G

S

S

L

L

N

T

G

G

K

L

R

P

C

S

D

F

R

H

S

Q

Y

H

F

F

D

D

Y

L

V

P

T

I

D

D

I

R

V

V

H

K

A

H

T

T

S

V

D

C

P

P

N

H

P

W

F

Y

K

N

R

A

P

P

Q

P

G

G

M

M

S

S

I

E

E

A

D

Q

F

F

C

V

D

A

L

Y

R

C

V

V

N

E

E

E

L

L

R

E

D

K

K

L

I

I

L

A

E

R

I

E

G

G

P

A

D

D

K

T

V

I

A

A

C

A

F

F

I

I

A

A

E
|
E

P

P

I

V

M

M

G

G

S

T

G

G

G

G

V

I

I

V

V

P

P

P

P

P

P

Q

G

G

Y

Y

H

W

K

E

K

A

T

I

L

Q

D

A

V

V

C

L

R

R

E

K

Y

H

D

D

V

I

L

L

Y

L

I

I

S

A

D
|
D

E

E

V
|
V

V

V

T

C

A

G

F

F

G

G

R

R

L

L

G

G

H

S

Y

K

F

T

A

G

S

S

E

E

E

H

V

-

F

Y

G

G

I

I

V

K

P

P

D

D

I

L

I

I

T

T

V

V

A

A

K
|
K

G

G

I

L

S

T

S

S

G

A

Y

Y

Q

A

P

P

L

L

G

S

A

A

A

V

I

I

F

V

S

S

Q

E

K

K

L

V

V

W

D

D

R

V

I

I

S

E

G

K

D

G

S

S

A

-

S

R

E

E

N

H

S

G

Y

V

Y

M

T

G

N

H

G

G

F
x
W

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

V

T

C

C

A

A

A

A

A

A

A

L

L

K

A

Y

N

L

L

E

D

I

I

M

L

K

E

R

R

D

E

K

N

L

L

T

T

D

G

H

N

V

A

L

A

E

D

V

V

G

G

P

A

Y

Y

F

L

M

Q

E

Q

R

R

L

L

-

H

K

E

T

A

L

F

K

G

E

A

L

H

S

P

V

L

V

V

G

G

D

E

V

V

R

R

G

G

H

V

C

G

L

M

M

L

A

A

C

A

V

L

E

E

C

F

V

M

V

A

S

D

D

K

N

G

E

A

-

R

-

T

-

P

-

F

D

D

E

P

N

A

I

L

A

K

V

V

A

S

Q

Q

R

K

V

V

D

A

E

A

F

A

C

A

Q

L

E

E

K
x
D

G

G

L

L

I

I

V

V

R

R

-

A

-

L

P

P

Y

H

E

G

N

D

L

I

C

L

I

G

L

F

S

A

P

P

P

P

L

L

I

V

I

T

E

T

K

R

K

A

H

E

A

V

D

D

Q

E

I

I

V

V

D

A

I

I

L

A

R

K

D
x
E

S

A

I

F

A

D

S

E

T

V

M

A

D

D

binding calcium ion: E66 (= E60), D70 (≠ Q64), D412 (≠ K402), E447 (≠ D435)
binding pyridoxal-5'-phosphate: S117 (= S110), G118 (= G111), S119 (= S112), Y151 (= Y144), H152 (= H145), G153 (= G146), E224 (= E217), D257 (= D250), V259 (= V252), K286 (= K280), W322 (≠ F317), T323 (= T318)

7qx3A Structure of the transaminase tr2e2 with eos (see paper)
38% identity, 94% coverage: 24:445/447 of query aligns to 1:422/422 of 7qx3A

query
sites
7qx3A

S

S

M

R

I

I

I

I

E

T

S

R

G

A

K

E

G

G

V

C

H

Y

V

L

F

W

D

D

S

S

E

D

G

G

N

N

K

Q

F

I

L

L

D

D

S

G

I

M

S

A

G

G

M

L

W

W

C

C

V

V

N

N

L

I

G

G

Y

Y

G

G

N

R

E

K

E

E

M

L

A

A

Q

E

A

V

I

A

A

Y

D

R

Q

Q

C

M

V

Q

K

E

L

L

A

P

Y

Y

Y

Y

T

N

P

N

F

F

G

F

A

Q

M

C

T

S

S

H

P

P

P

P

S

A

I

I

Q

E

L

L

A

S

T

R

E

L

L

L

S

S

A

E

L

V

T

T

P

P

G

K

D

H

L

M

N

N

C

H

F

V

Q

F

F

F

T

T

N

G

S

S

G

G

S

S

T

D

A

S

V

N

E

D

S

T

A

I

I

L

R

R

F

M

V

V

H

R

Y

Y

Y

Y

F

W

N

K

C

L

L

L

G

G

Q

K

P

P

E

Y

K

K

K

K

H

V

I

V

I

I

Y

S

R

R

E

E

N

N

A

A

Y
|
Y

H

H

G

G

S

S

T

T

Y

V

L

A

A

G

A

A

S

S

L

L

N

S

G

G

K

M

R

K

C

A

D

M

R

H

S

S

Y

H

F

G

D

D

Y

L

V

P

T

I

D

P

I

G

V

I

H

E

A

H

T

I

S

E

D

Q

P

P

N

Y

P

H

F

F

K

G

R

R

P

A

Q

P

G

D

M

M

S

D

I

P

E

A

D

E

F

F

C

G

D

R

L

Q

R

A

V

A

N

Q

E

A

L

L

R

E

D

R

K

K

I

I

L

D

E

E

I

I

G

G

P

E

D

C

K

N

V

V

A

A

C

A

F

F

I

I

A

A

E

E

P

P

I

I

M

Q

G

G

S
x
A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

P

D

G

S

Y

Y

H

W

K

P

K

E

T

I

L

K

D

R

V

I

C

C

R

A

E

E

Y

R

D

D

V

I

L

L

Y

L

I

I

S

V

D

D

E

E

V

V

V

I

T

T

A

G

F

F

G

G

R

R

L

L

G

G

H

T

Y

W

F

F

A

G

S

S

E

-

E

Q

V

Y

F

Y

G

D

I

L

V

Q

P

P

D

D

I

L

I

M

T

P

V

I

A

A

K
|
K

G

G

I

L

S

S

S

S

G

G

Y

Y

Q

M

P

P

L

I

G

G

A

G

A

V

I

M

F

V

S

S

Q

-

K

-

L

-

V

-

D

D

R

R

I

V

S

A

G

K

D

V

S

V

A

I

S

E

E

E

N

G

S

G

Y

E

Y

F

T

F

N

H

G

G

F

Y

T

T

Y

Y

S

S

G

G

H

H

P

P

V

V

T

A

C

A

A

A

A

V

A

A

L

A

K

E

Y

N

L

I

E

R

I

I

M

M

K

R

R

D

D

E

K

G

L

I

T

I

D

E

H

R

V

A

-

G

L

A

E

E

V

I

G

A

P

P

Y

Y

F

L

M

Q

E

A

R

R

L

W

K

R

T

E

L

L

K

G

E

E

L

H

S

P

V

L

V

V

G

G

D

E

V

A

R

R

G

G

H

V

C

G

L

M

M

V

A

A

C

A

V

L

E

E

C

L

V

V

V

K

S

S

D

K

N

Q

E

P

D

L

E

E

N

R

I

F

A

E

V

E

A

P

Q

G

R

K

V

V

D

G

E

S

F

L

C

C

Q

R

E

D

-

L

-

S

-

V

-

K

K

N

G

G

L

L

I

V

V

M

R

R

P

A

Y

V

E

G

N

G

L

T

C

M

I

I

L

I

S

S

P

P

P

P

L

L

I

V

I

L

E

S

K

R

K

E

H

Q

A

V

D

D

Q

E

I

L

V

I

D

D

I

K

L

A

R

R

D

R

S

T

I

L

A

D

S

E

T

T

M

H

D

K

E

A

M

I

G

G

binding 2-azanylethyl hydrogen sulfate: Y121 (= Y144), A199 (≠ S222), K256 (= K280)

6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
37% identity, 99% coverage: 2:442/447 of query aligns to 7:448/448 of 6io1B

query
sites
6io1B

N

E

L

L

A

V

G

A

K

K

D

D

K

I

K

A

H

H

I

H

I

L

H

H

P

P

W
x
L

S

T

N

N

L

L

G

Y

S

Q

D

L

A

R

E

R

S

E

-

G

-

P

M

L

I

V

I

L

E

V

S

R

G

G

K

E

G

G

V

V

H

W

V

V

F

W

D

D

S

A

E

E

G

G

N

K

K

R

F

Y

L

L

D

D

S

G

I

F

S

A

G

G

M

L

W

W

C

N

V

V

N

N

L

I

G

G

Y

H

G

G

N

R

E

R

E

E

M

L

A

A

Q

E

A

A

I

A

A

R

D

E

Q

Q

C

M

V

E

K

R

L

V

A

A

Y

F

Y

V

T

P

P

T

F

F

G

F

A

G

M

L

T

A

S

S

P

P

P

P

S

T

I

I

Q

E

L

L

A

A

T

A

E

R

L

L

S

A

A

E

L

L

T

F

P

P

G

G

D

P

L

L

N

D

C

H

F

F

Q

Q

F

F

T

T

N

S

S
x
G

G
|
G

S
x
A

T

E

A

S

V
x
N

E

E

S

T

A

A

I

I

R

K

F

I

V

A

H

R

Y

Y

Y

Y

F

W

N

W

C

L

L

K

G

G

Q

Q

P

P

E

E

K

R

K

V

H

K

I

I

I

L

Y

S

R

R

E

R

N

M

A

A

Y
|
Y

H
|
H

G

G

S

I

T

A

Y

M

L

G

A

A

A

L

S

S

L

A

N

T

G

G

K

V

R

P

C

A

D

Y

R

W

S

E

Y

G

F

F

D

G

-

P

Y

R

V

P

T

P

D

G

I

F

V

I

H

H

A

L

T

T

S

A

D

-

P

P

N

Y

P

K

F

Y

K

R

R

F

P

G

Q

E

G

G

M

L

S

T

I

D

E

E

D

E

F

F

C

V

D

A

L

R

R

L

V

V

N

Q

E

E

L

L

R

E

D

E

K

T

I

I

L

E

E

R

I

E

G

G

P

S

D

E

K

T

V

I

A

A

C

A

F

F

I

I

A

G

E

E

P

P

I

V

M

Q

G

G

S

A

G

G

G

G

V

V

I

V

V

V

P

P

P

P

P

D

G

G

Y

Y

H

W

K

P

K

A

T

I

L

A

D

A

V

V

C

L

R

R

E

K

Y

Y

D

G

V

I

L

L

Y

L

I

I

S

L

D
|
D

E

E

V
|
V

V
x
I

T

T

A

G

F

F

G

G

R

R

L

T

G

G

H

T

Y

L

F

F

A

G

S

M

E

Q

E

Q

V

-

F

Y

G

G

I

V

V

Q

P

P

D

D

I

I

I

V

T

T

V

F

A

A

K
|
K

G

G

I

I

S

T

S

S

G

G

Y

Y

Q

V

P

P

L

L

G

G

A

G

A

V

I

G

F

V

S

S

Q

-

K

-

L

-

V

-

D

D

R

E

I

I

S

A

G

E

D

T

S

L

A

A

S

S

E

A

N

D

S

R

Y

V

Y

F

T

M

N

H

G

G

F

F

T

T

Y

Y

S

S

G

G

H

H

P

P

V

V

T

A

C

C

A

A

A

V

A

A

L

L

K

R

Y

N

L

L

E

D

I

I

M

L

K

L

R

A

D

E

K

R

L

L

T

W

D

E

H

N

V

A

L

A

E

A

V

S

G

G

P

A

Y

Y

F

L

M

L

E

Q

R

E

L

L

K

K

T

R

L

L

K

E

E

E

L

R

S

P

V

Y

V

V

G

G

D

E

V

V

R

R

G

G

H

K

C

G

L

L

M

M

A

L

C

L

V

V

E

E

C

V

V

V

V

R

S

D

D

K

N

A

E

S

D

K

E

E

N

K

-

F

-

P

-

P

-

E

I

F

A

K

V

L

A

G

Q

P

R

K

V

L

D

E

E

A

F

A

C

T

Q

R

E

R

K

R

G

G

L

I

I

I

V

V

R

R

P

C

Y

T

E

P

N

D

L

G

C

I

I

V

L

M

S

A

P

P

P

P

L

L

I

T

I

I

E

S

K

R

K

A

H

E

A

C

D

D

Q

V

I

L

V

I

D

E

I

G

L

V

R

A

D

A

S

A

I

L

A

S

S

D

T

V

M

L

D

D

active site: L20 (≠ W15), Y151 (= Y144), D257 (= D250), K286 (= K280)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ S110), G118 (= G111), A119 (≠ S112), N122 (≠ V115), Y151 (= Y144), H152 (= H145), D257 (= D250), V259 (= V252), I260 (≠ V253), K286 (= K280)

5kquC Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
38% identity, 98% coverage: 6:444/447 of query aligns to 10:458/459 of 5kquC

query
sites
5kquC

K

Q

D

D

K

R

K

A

H

H

I

F

I

M

H

H

P

P

W

S

S

T

N

H

L

A

G

H

S

D

D

H

A

A

E

S

S

G

M

A

-

L

-

P

-

G

-

R

I

I

I

I

E

T

S

G

G

A

K

S

G

G

V

I

H

R

V

I

F

R

D

D

S

H

E

E

G

G

N

R

K

E

F

L

L

I

D

D

S

A

I

F

S

A

G

G

M

L

W

Y

C

C

V

V

N

N

L

I

G

G

Y

Y

G

G

N

R

E

T

E

E

M

V

A

A

Q

D

A

A

I

I

A

Y

D

K

Q

Q

C

A

V

K

K

E

L

L

A

A

Y

Y

Y

Y

T

H

P

T

F

Y

G

V

A

G

M

H

T

S

S

T

P

E

P

A

S

I

I

I

Q

E

L

L

A

S

T

S

E

R

L

I

-

I

S

R

A

D

L

W

T

A

P

P

G

A

D

G

L

M

N

K

C

K

F

V

Q

Y

F

Y

T

G

N

L

S
|
S

G
|
G

S
|
S

T

D

A

A

V

N

E

E

S

T

A

Q

I

I

R

K

F

L

V

V

H

R

Y

Y

Y

Y

F

N

N

N

C

V

L

L

G

G

Q

R

P

P

E

Q

K

K

K

K

H

K

I

I

I

I

Y

S

R

R

E

Q

N

R

A

G

Y
|
Y

H
|
H

G

G

S

S

T

G

Y

I

L

M

A

T

A

G

S

S

L

L

N

T

G

G

K

L

R

P

C

S

D

F

R

H

S

Q

Y

H

F

F

D

D

Y

L

V

P

T

V

D

E

I

G

V

I

H

K

A

H

T

T

S

V

D

C

P

P

N

H

P

W

F

Y

K

K

R

A

P

P

Q

A

G

G

M

M

S

D

I

E

E

A

D

A

F

F

C

V

D

R

L

Y

R

C

V

A

N

D

E

E

L

L

R

E

D

K

K

L

I

I

L

L

E

A

I

E

G

G

P

P

D

D

K

T

V

V

A

A

C

A

F

F

I

I

A

G

E

E

P

P

I

V

M

M

G

G

S

T

G

G

G

G

V

I

I

I

V

V

P

P

P

P

P

K

G

G

Y

Y

H

W

K

E

K

A

T

I

L

Q

D

A

V

V

C

L

R

N

E

K

Y

Y

D

D

V

V

L

L

Y

L

I

I

S

A

D
|
D

E

E

V
|
V

V

V

T

C

A

A

F

F

G

G

R

R

L

L

G

G

H

S

Y

K

F

M

A

G

S

S

E

Q

E

R

V

-

F

Y

G

G

I

M

V

R

P

P

D

D

I

L

I

I

T

T

V

T

A

A

K
|
K

G

G

I

L

S

T

S

S

G

A

Y

Y

Q

A

P

P

L

L

G

S

A

A

A

V

I

I

F

V

S

G

Q

E

K

K

L

V

V

W

D

D

R

V

I

I

S

E

G

K

D

A

S

S

A

Q

S

K

E

E

N

G

S

A

Y

-

Y

M

T

G

N

H

G

G

F
x
W

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

I

T

C

C

A

A

A

A

A

A

A

L

L

K

A

Y

N

L

L

E

D

I

I

M

L

K

E

R

R

D

E

K

N

L

L

T

T

D

A

H

N

V

A

L

A

E

D

V

V

G

G

P

A

Y

Y

F

L

M

N

E

Q

R

R

L

L

K

R

-

E

T

T

L

F

K

E

E

G

L

H

S

P

V

L

V

V

G

G

D

E

V

V

R

R

G

G

H

D

C

G

L

M

M

L

A

A

C

A

V

L

E

E

C

-

V

F

V

M

S

A

D

D

N

R

E

E

-

A

-

R

-

T

-

P

-

F

D

D

E

P

N

A

I

L

A

K

V

V

A

G

Q

P

R

K

V

V

D

S

E

A

F

A

C

C

Q

L

E

E

K

D

G

G

L

M

I

I

V

A

R

R

-

A

-

M

P

P

Y

H

E

G

N

D

L

I

C

L

I

G

L

F

S

A

P

P

P

P

L

L

I

V

I

L

E

T

K

R

K

A

H

E

A

A

D

D

Q

E

I

I

V

V

D

G

I

I

L

A

R

K

D

A

S

A

I

V

A

D

S

E

T

V

M

A

D

G

E

E

M

V

binding pyridoxal-5'-phosphate: S119 (= S110), G120 (= G111), S121 (= S112), Y153 (= Y144), H154 (= H145), D259 (= D250), V261 (= V252), K288 (= K280), W324 (≠ F317), T325 (= T318)

7qx0B Transaminase structure of plurienzyme (tr2e2) in complex with plp (see paper)
38% identity, 98% coverage: 7:445/447 of query aligns to 9:443/443 of 7qx0B

query
sites
7qx0B

D

D

K

A

K

A

H

H

I

H

I

L

H

H

P

P

W

F

S

T

N

D

L

Y

G

G

-

E

-

L

S

N

D

T

A

K

E

G

S

S

M

R

I

I

I

I

E

T

S

R

G

A

K

E

G

G

V

C

H

Y

V

L

F

W

D

D

S

S

E

D

G

G

N

N

K

Q

F

I

L

L

D

D

S

G

I

M

S

A

G

G

M

L

W

W

C

C

V

V

N

N

L

I

G

G

Y

Y

G

G

N

R

E

K

E

E

M

L

A

A

Q

E

A

V

I

A

A

Y

D

R

Q

Q

C

M

V

Q

K

E

L

L

A

P

Y

Y

Y

Y

T

N

P

N

F

F

G

F

A

Q

M

C

T

S

S

H

P

P

P

P

S

A

I

I

Q

E

L

L

A

S

T

R

E

L

L

L

S

S

A

E

L

V

T

T

P

P

G

K

D

H

L

M

N

N

C

H

F

V

Q

F

F

F

T

T

N

G

S
|
S

G
|
G

S
|
S

T

D

A

S

V

N

E

D

S

T

A

I

I

L

R

R

F

M

V

V

H

R

Y

Y

Y

Y

F

W

N

K

C

L

L

L

G

G

Q

K

P

P

E

Y

K

K

K

K

H

V

I

V

I

I

Y

S

R

R

E

E

N

N

A

A

Y
|
Y

H
|
H

G

G

S

S

T

T

Y

V

L

A

A

G

A

A

S

S

L

L

N

S

G

G

K

M

R

K

C

A

D

M

R

H

S

S

Y

H

F

G

D

D

Y

L

V

P

T

I

D

P

I

G

V

I

H

E

A

H

T

I

S

E

D

Q

P

P

N

Y

P

H

F

F

K

G

R

R

P

A

Q

P

G

D

M

M

S

D

I

P

E

A

D

E

F

F

C

G

D

R

L

Q

R

A

V

A

N

Q

E

A

L

L

R

E

D

R

K

K

I

I

L

D

E

E

I

I

G

G

P

E

D

C

K

N

V

V

A

A

C

A

F

F

I

I

A

A

E
|
E

P

P

I

I

M

Q

G

G

S

A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

P

D

G

S

Y

Y

H

W

K

P

K

E

T

I

L

K

D

R

V

I

C

C

R

A

E

E

Y

R

D

D

V

I

L

L

Y

L

I

I

S

V

D
|
D

E

E

V
|
V

V
x
I

T

T

A

G

F

F

G

G

R

R

L

L

G

G

H

T

Y

W

F

F

A

G

S

S

E

-

E

Q

V

Y

F

Y

G

D

I

L

V

Q

P

P

D

D

I

L

I

M

T

P

V

I

A

A

K
|
K

G

G

I

L

S

S

S

S

G

G

Y

Y

Q

M

P

P

L

I

G

G

A

G

A

V

I

M

F

V

S

S

Q

-

K

-

L

-

V

-

D

D

R

R

I

V

S

A

G

K

D

V

S

V

A

I

S

E

E

E

N

-

S

-

Y

-

Y

-

T

-

N

-

G

G

F

G

T

E

Y

Y

S

S

G

G

H

H

P

P

V

V

T

A

C

A

A

A

A

V

A

A

L

A

K

E

Y

N

L

I

E

R

I

I

M

M

K

R

R

D

D

E

K

G

L

I

T

I

D

E

H

R

V

A

-

G

L

A

E

E

V

I

G

A

P

P

Y

Y

F

L

M

Q

E

A

R

R

L

W

K

R

T

E

L

L

K

G

E

E

L

H

S

P

V

L

V

V

G

G

D

E

V

A

R

R

G

G

H

V

C

G

L

M

M

V

A

A

C

A

V

L

E

E

C

L

V

V

V

K

S

S

D

K

N

Q

E

P

D

L

E

E

N

R

I

F

A

E

V

E

A

P

Q

G

R

K

V

V

D

G

E

S

F

L

C

C

Q

R

E

D

-

L

-

S

-

V

-

K

K

N

G

G

L

L

I

V

V

M

R

R

P

A

Y

V

E

G

N

G

L

T

C

M

I

I

L

I

S

S

P

P

P

P

L

L

I

V

I

L

E

S

K

R

K

E

H

Q

A

V

D

D

Q

E

I

L

V

I

D

D

I

K

L

A

R

R

D

R

S

T

I

L

A

D

S

E

T

T

M

H

D

K

E

A

M

I

G

G

binding pyridoxal-5'-phosphate: S114 (= S110), G115 (= G111), S116 (= S112), Y148 (= Y144), H149 (= H145), E221 (= E217), D254 (= D250), V256 (= V252), I257 (≠ V253), K283 (= K280)

7ypmA Crystal structure of transaminase cc1012 complexed with plp and l- alanine (see paper)
39% identity, 93% coverage: 24:437/447 of query aligns to 30:444/454 of 7ypmA

query
sites
7ypmA

S

S

M

R

I

I

I

I

E

T

S

R

G

A

K

E

G

G

V

V

H

Y

V

I

F

H

D

D

S

G

E

E

G

G

N

H

K

Q

F

I

L

L

D

D

S

G

I

M

S

A

G

G

M

L

W
|
W

C

C

V

V

N

N

L

V

G

G

Y

Y

G

G

N

R

E

E

E

E

M

L

A

A

Q

K

A

A

I

A

A

Y

D

D

Q

Q

C

M

V

L

K

E

L

L

A

P

Y

Y

Y

Y

T

N

P

T

F

F

G

F

A

K

M

T

T

A

S

T

P

P

P

P

S

P

I

I

Q

E

L

L

A

A

T

A

E

K

L

I

S

A

A

Q

L

K

T

M

P

G

G

G

D

H

L

L

N

S

C

H

F

V

Q

F

F

Y

T

N

N

S

S
|
S

G
|
G

S
|
S

T

E

A

A

V

N

E

D

S

T

A

V

I

F

R

R

F

L

V

V

H

R

Y

H

Y

F

F

W

N

K

C

L

L

K

G

G

Q

E

P

P

E

S

K

R

K

T

H

V

I

F

I

I

Y

S

R

R

E

W

N

N

A

A

Y
|
Y

H

H

G
|
G

S

S

T

T

Y

V

L

A

A

G

A

V

S

S

L

L

N

G

G

G

K

M

R

K

C

H

D

M

R

H

S

K

Y

Q

F

G

D

D

Y

L

V

P

T

I

D

A

I

G

V

V

H

E

A

H

T

V

S

M

D

Q

P

P

N

Y

P

Q

F

F

K

G

R

D

P

G

Q

F

G

G

M

E

S

D

I

P

E

A

D

A

F

F

C

R

D

D

L

R

R

A

V

V

N

Q

E

A

L

I

R

E

D

D

K

K

I

I

L

L

E

E

I

V

G

G

P

P

D

E

K

N

V

V

A

A

C

A

F

F

I

I

A

G

E
|
E

P

P

I

V

M

Q

G

G

S

A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

P

D

G

G

Y

Y

H

W

K

P

K

A

T

V

L

E

D

A

V

L

C

C

R

R

E

K

Y

Y

D

G

V

I

L

L

Y

L

I

V

S

C

D
|
D

E

E

V
|
V

V
x
I

T

C

A

G

F

F

G

G

R

R

L

L

G

G

H

Q

Y

W

F

F

A

G

S

H

E

Q

E

H

V

-

F

Y

G

G

I

I

V

K

P

P

D

D

I

L

I

I

T

A

V

M

A

A

K
|
K

G

G

I

L

S

S

S

S

G

G

Y

Y

Q

L

P

P

L

I

G

S

A

A

A

V

I

-

F

-

S

-

Q

-

K

G

L

V

V

A

D

D

R

H

I

I

S

V

G

A

D

E

S

L

A

R

S

E

E

K

N

G

S

G

Y

D

Y

F

T

I

N

H

G

G

F

F

T

T

Y

Y

S

S

G

G

H

H

P

P

V

T

T

A

C

A

A

A

A

V

A

A

L

L

K

K

Y

N

L

I

E

E

I

I

M

M

K

E

R

R

D

E

K

G

L

L

T

V

D

E

H

R

V

T

L

R

-

D

E

E

V

T

G

G

P

P

Y

Y

F

L

M

A

E

Q

R

A

L

L

K

A

T

S

L

L

K

N

E

D

L

H

S

P

V

L

V

V

G

G

D

E

V

V

R

R

G

S

H

L

C

G

L

L

M

I

A

G

C

A

V

V

E

E

C

I

V

V

V

R

S

E

D

K

N

G

E

T

-

N

-

H

-

R

-

F

-

L

D

D

E

K

N

E

I

G

A

E

V

A

A

G

Q

P

R

I

V

V

D

R

E

D

F

L

C

C

Q

I

E

K

K

N

G

G

L

L

I

M

V

V

R

R

P

A

Y

I

E

R

N

D

L

S

C

I

I

V

L

C

S

C

P

P

P

P

L

L

I

I

I

I

E

T

K

K

K

A

H

Q

A

I

D

D

Q

E

I

L

V

V

D

G

I

I

L

I

R

R

D

K

S

S

I

L

binding alanine: W57 (= W51), Y150 (= Y144)
binding pyridoxal-5'-phosphate: S116 (= S110), G117 (= G111), S118 (= S112), Y150 (= Y144), G152 (= G146), E223 (= E217), D256 (= D250), V258 (= V252), I259 (≠ V253), K285 (= K280)

7ypnD Crystal structure of transaminase cc1012 mutant m9 complexed with plp (see paper)
39% identity, 93% coverage: 24:437/447 of query aligns to 30:444/455 of 7ypnD

query
sites
7ypnD

S

S

M

R

I

I

I

I

E

T

S

R

G

A

K

E

G

G

V

V

H

Y

V

I

F

H

D

D

S

G

E

E

G

G

N

H

K

Q

F

I

L

L

D

D

S

G

I

W

S

A

G

G

M

L

W

F

C

C

V

V

N

N

L

V

G

G

Y

Y

G

G

N

R

E

E

E

E

M

L

A

A

Q

K

A

A

I

A

A

Y

D

D

Q

Q

C

M

V

L

K

E

L

L

A

P

Y

Y

Y

Y

T

N

P

T

F

F

G

F

A

K

M

T

T

A

S

T

P

P

P

P

S

P

I

I

Q

E

L

L

A

A

T

A

E

K

L

I

S

A

A

Q

L

K

T

M

P

G

G

G

D

H

L

L

N

S

C

H

F

V

Q

F

F

Y

T

N

N

S

S
|
S

G
|
G

S
|
S

T

E

A

A

V

N

E

D

S

T

A

V

I

F

R

R

F

L

V

V

H

R

Y

H

Y

F

F

W

N

K

C

L

L

K

G

G

Q

E

P

P

E

S

K

R

K

T

H

V

I

F

I

I

Y

S

R

R

E

W

N

N

A

A

Y
|
Y

H
|
H

G
|
G

S

S

T

T

Y

V

L

A

A

G

A

V

S

S

L

L

N

G

G

G

K

M

R

K

C

H

D

M

R

H

S

K

Y

Q

F

G

D

D

Y

L

V

P

T

I

D

A

I

G

V

V

H

E

A

H

T

V

S

M

D

Q

P

P

N

Y

P

Q

F

F

K

G

R

D

P

G

Q

F

G

G

M

E

S

D

I

P

E

A

D

A

F

F

C

R

D

D

L

R

R

A

V

V

N

Q

E

A

L

I

R

E

D

D

K

K

I

I

L

L

E

E

I

V

G

G

P

P

D

E

K

N

V

V

A

A

C

A

F

F

I

I

A

G

E
|
E

P

P

I

V

M

Q

G

G

S

A

G

G

G

G

V

V

I

I

V

I

P

P

P

P

P

D

G

G

Y

Y

H

W

K

P

K

A

T

V

L

E

D

A

V

L

C

C

R

R

E

K

Y

Y

D

G

V

I

L

L

Y

L

I

V

S

C

D
|
D

E

E

V
|
V

V

A

T

C

A

G

F

F

G

G

R

R

L

L

G

G

H

Q

Y

W

F

F

A

G

S

H

E

Q

E

H

V

-

F

Y

G

G

I

I

V

K

P

P

D

D

I

L

I

I

T

A

V

M

A

A

K
|
K

G

G

I

L

S

S

S

S

G

G

Y

Y

Q

L

P

P

L

I

G

S

A

A

A

V

I

-

F

-

S

-

Q

-

K

G

L

V

V

A

D

D

R

H

I

I

S

V

G

A

D

E

S

L

A

R

S

E

E

K

N

G

S

G

Y

D

Y

F

T

I

N

H

G

G

F
|
F

T
|
T

Y

Y

S

S

G

G

H

H

P

P

V

T

T

A

C

A

A

A

A

V

A

A

L

L

K

K

Y

N

L

I

E

E

I

I

M

M

K

E

R

R

D

E

K

G

L

L

T

V

D

E

H

R

V

T

L

R

-

D

E

E

V

T

G

G

P

P

Y

Y

F

L

M

A

E

Q

R

A

L

L

K

A

T

S

L

L

K

N

E

D

L

H

S

P

V

L

V

V

G

G

D

E

V

V

R

R

G

S

H

L

C

G

L

L

M

I

A

G

C

A

V

V

E

E

C

I

V

V

V

R

S

E

D

K

N

G

E

T

-

N

-

H

-

R

-

F

-

L

D

D

E

K

N

E

I

G

A

E

V

A

A

G

Q

P

R

I

V

V

D

R

E

D

F

L

C

C

Q

I

E

K

K

N

G

G

L

L

I

M

V

V

R

R

P

A

Y

I

E

R

N

D

L

S

C

I

I

V

L

C

S

C

P

P

P

P

L

L

I

I

I

I

E

T

K

K

K

A

H

Q

A

I

D

D

Q

E

I

L

V

V

D

G

I

I

L

I

R

R

D

K

S

S

I

L

binding pyridoxal-5'-phosphate: S116 (= S110), G117 (= G111), S118 (= S112), Y150 (= Y144), H151 (= H145), G152 (= G146), E223 (= E217), D256 (= D250), V258 (= V252), K285 (= K280), F318 (= F317), T319 (= T318)

5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
38% identity, 98% coverage: 7:444/447 of query aligns to 12:457/458 of 5kr3A

query
sites
5kr3A

D

D

K

R

K

A

H

H

I

F

I

M

H

H

P

P

W

S

S

T

N

H

L

A

G

H

S

D

D

H

A

A

E

S

S

G

M

A

-

L

-

P

-

G

-

R

I

I

I

I

E

T

S

G

G

G

K

K

G

G

V

I

H

R

V

I

F

E

D

D

S

H

E

E

G

G

N

R

K

E

F

Y

L

I

D

D

S

A

I

F

S

A

G

G

M

L

W

Y

C

C

V

V

N

N

L

I

G

G

Y

Y

G

G

N

R

E

T

E

E

M

V

A

A

Q

D

A

A

I

I

A

Y

D

E

Q

Q

C

A

V

K

K

Q

L

L

A

A

Y

Y

Y

Y

T

H

P

T

F

Y

G

V

A

G

M

H

T

S

S

T

P

D

P

A

S

I

I

I

Q

E

L

L

A

S

T

S

E

R

L

I

-

I

S

R

A

D

L

W

T

A

P

P

G

A

D

G

L

M

N

K

C

K

F

V

Q

Y

F

Y

T

G

N

M

S
|
S

G
|
G

S
|
S

T

D

A

A

V

N

E

E

S

T

A

Q

I

I

R

K

F

L

V

V

H

W

Y

Y

Y

Y

F

N

N

N

C

V

L

L

G

G

Q

R

P

P

E

N

K

K

K

K

H

K

I

I

I

I

Y

S

R

R

E

E

N

R

A

G

Y
|
Y

H
|
H

G

G

S

S

T

G

Y

I

L

V

A

T

A

G

S

S

L

L

N

T

G

G

K

L

R

P

C

S

D

F

R

H

S

Q

Y

H

F

F

D

D

Y

L

V

P

T

I

D

D

I

R

V

V

H

K

A

H

T

T

S

V

D

C

P

P

N

H

P

W

F

Y

K

R

R

A

P

P

Q

-

G

-

M

M

S

S

I

E

E

A

D

Q

F

F