SitesBLAST
Comparing WP_035235693.1 NCBI__GCF_000745975.1:WP_035235693.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1ji0A Crystal structure analysis of the abc transporter from thermotoga maritima
41% identity, 97% coverage: 1:223/231 of query aligns to 6:229/240 of 1ji0A
1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 98% coverage: 1:226/231 of query aligns to 4:243/254 of 1g6hA
1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
31% identity, 98% coverage: 1:226/231 of query aligns to 4:243/253 of 1g9xB
6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
34% identity, 93% coverage: 1:215/231 of query aligns to 2:218/240 of 6mjpA
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
34% identity, 92% coverage: 4:215/231 of query aligns to 5:215/374 of 2awnB
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
34% identity, 92% coverage: 4:215/231 of query aligns to 5:215/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y11), S37 (≠ N36), G38 (= G37), C39 (≠ V38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), Q81 (= Q83), R128 (= R128), A132 (≠ D132), S134 (= S134), G136 (= G136), Q137 (= Q137), E158 (= E158), H191 (≠ Q191)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q83)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
34% identity, 92% coverage: 4:215/231 of query aligns to 5:215/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), G38 (= G37), C39 (≠ V38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R128), S134 (= S134), Q137 (= Q137)
- binding beryllium trifluoride ion: S37 (≠ N36), G38 (= G37), K41 (= K40), Q81 (= Q83), S134 (= S134), G136 (= G136), H191 (≠ Q191)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q83)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
34% identity, 92% coverage: 4:215/231 of query aligns to 5:215/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ T16), G38 (= G37), C39 (≠ V38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R128), A132 (≠ D132), S134 (= S134), Q137 (= Q137)
- binding tetrafluoroaluminate ion: S37 (≠ N36), G38 (= G37), K41 (= K40), Q81 (= Q83), S134 (= S134), G135 (= G135), G136 (= G136), E158 (= E158), H191 (≠ Q191)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q83)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
34% identity, 92% coverage: 4:215/231 of query aligns to 5:215/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ T16), G38 (= G37), C39 (≠ V38), G40 (= G39), K41 (= K40), S42 (≠ T41), T43 (= T42), R128 (= R128), A132 (≠ D132), S134 (= S134), Q137 (= Q137)
- binding magnesium ion: S42 (≠ T41), Q81 (= Q83)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
34% identity, 92% coverage: 4:215/231 of query aligns to 6:216/371 of P68187
- A85 (≠ Q86) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ G107) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V115) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L118) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ P120) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (vs. gap) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G136) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D157) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
Sites not aligning to the query:
- 228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
34% identity, 93% coverage: 2:215/231 of query aligns to 1:213/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ Y11), S35 (≠ N36), G36 (= G37), C37 (≠ V38), G38 (= G39), K39 (= K40), S40 (≠ T41), T41 (= T42), R126 (= R128), A130 (≠ D132), S132 (= S134), G134 (= G136), Q135 (= Q137)
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
29% identity, 95% coverage: 1:220/231 of query aligns to 2:223/241 of 4u00A
6b89A E. Coli lptb in complex with adp and novobiocin (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/234 of 6b89A
- binding adenosine-5'-diphosphate: Y12 (= Y11), R15 (≠ S14), V17 (≠ T16), N37 (= N36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42)
- binding magnesium ion: T42 (= T41), Q84 (= Q83)
- binding novobiocin: L71 (≠ K68), H72 (≠ R69), P83 (= P82), A86 (≠ R85), S87 (≠ Q86), F89 (= F88), R90 (≠ P89), R91 (≠ L90), L92 (= L91), V101 (≠ G100), Q135 (≠ G131), R149 (= R145)
4p31A Crystal structure of a selenomethionine derivative of e. Coli lptb in complex with adp-magensium (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/234 of 4p31A
6b8bA E. Coli lptb in complex with adp and a novobiocin derivative (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/233 of 6b8bA
- binding adenosine-5'-diphosphate: Y12 (= Y11), R15 (≠ S14), V17 (≠ T16), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42)
- binding (3s,5s,7s)-N-{7-[(3-O-carbamoyl-6-deoxy-5-methyl-4-O-methyl-beta-D-gulopyranosyl)oxy]-4-hydroxy-8-methyl-2-oxo-2H-1-benzopyran-3-yl}tricyclo[3.3.1.1~3,7~]decane-1-carboxamide: F89 (= F88), R90 (≠ P89), R91 (≠ L90)
6s8nB Cryo-em structure of lptb2fgc in complex with lipopolysaccharide (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/238 of 6s8nB
6s8gA Cryo-em structure of lptb2fgc in complex with amp-pnp (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/238 of 6s8gA
- binding phosphoaminophosphonic acid-adenylate ester: Y12 (= Y11), R15 (≠ S14), N37 (= N36), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42), Q84 (= Q83), S136 (≠ D132), S138 (= S134), E141 (≠ Q137)
6mhzA Vanadate trapped cryo-em structure of e.Coli lptb2fg transporter (see paper)
32% identity, 96% coverage: 2:223/231 of query aligns to 3:226/235 of 6mhzA
- binding adp orthovanadate: Y12 (= Y11), N37 (= N36), G38 (= G37), G40 (= G39), K41 (= K40), T42 (= T41), T43 (= T42), Q84 (= Q83), S136 (≠ D132), S138 (= S134), G139 (= G135), G140 (= G136), E162 (= E158), G166 (= G162), H194 (≠ Q191)
6mbnA Lptb e163q in complex with atp (see paper)
31% identity, 96% coverage: 2:223/231 of query aligns to 4:227/241 of 6mbnA
3c4jA Abc protein artp in complex with atp-gamma-s
30% identity, 96% coverage: 1:222/231 of query aligns to 3:227/242 of 3c4jA
Query Sequence
>WP_035235693.1 NCBI__GCF_000745975.1:WP_035235693.1
MLTIENLNQYYGESHTLWDLSLTIEEKCCTCLMGRNGVGKTTLLNCIMGLVPVKSGAISF
QGNNLLKKRAEDRAGSGIGYVPQGRQIFPLLTVEENLTIGLTTVGKGKKKIPDMVFELFP
VLQEMLKRRGGDLSGGQQQQLAIGRALVLDPSLLILDEPCEGIQPNIVQEIGEIIKRLIQ
EVGMAVLLVEQKLHFVKQTADHFFIMDRGRLMAGGNISQLTDDLISKYLTV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory