SitesBLAST

Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
49% identity, 89% coverage: 1:140/157 of query aligns to 4:144/157 of Q0QLF3

query
sites
Q0QLF3

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C42 (= C39) binding [2Fe-2S] cluster
C47 (= C44) binding [2Fe-2S] cluster
C50 (= C47) binding [2Fe-2S] cluster
C62 (= C59) binding [2Fe-2S] cluster
C101 (= C97) binding [2Fe-2S] cluster
C104 (= C100) binding [2Fe-2S] cluster
C136 (= C132) binding [2Fe-2S] cluster
C138 (= C134) binding [2Fe-2S] cluster

8uemC The cryoem structure of the high affinity carbon monoxide dehydrogenase from mycobacterium smegmatis (see paper)
47% identity, 95% coverage: 1:149/157 of query aligns to 1:150/153 of 8uemC

query
sites
8uemC

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binding fe2/s2 (inorganic) cluster: W37 (≠ E37), G38 (= G38), C39 (= C39), S42 (≠ G42), N43 (≠ E43), C44 (= C44), G45 (= G45), C47 (= C47), K57 (≠ L57), C59 (= C59), C98 (= C97), G99 (= G98), C101 (= C100), C133 (= C132), C135 (= C134)
binding pterin cytosine dinucleotide: Q97 (= Q96), C135 (= C134)

1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
50% identity, 87% coverage: 18:153/157 of query aligns to 19:155/155 of 1ffvA

query
sites
1ffvA

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binding fe2/s2 (inorganic) cluster: I38 (≠ E37), C40 (= C39), S43 (≠ G42), C45 (= C44), G46 (= G45), C48 (= C47), C60 (= C59), C99 (= C97), G100 (= G98), C102 (= C100), C134 (= C132), C136 (= C134)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q96), C136 (= C134)

1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
50% identity, 87% coverage: 18:153/157 of query aligns to 20:156/156 of 1ffuD

query
sites
1ffuD

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binding fe2/s2 (inorganic) cluster: C41 (= C39), S44 (≠ G42), H45 (≠ E43), C46 (= C44), G47 (= G45), C49 (= C47), C61 (= C59), C100 (= C97), G101 (= G98), C103 (= C100), C135 (= C132), C137 (= C134)

8gy3B Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
44% identity, 97% coverage: 1:153/157 of query aligns to 1:153/154 of 8gy3B

query
sites
8gy3B

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binding fe2/s2 (inorganic) cluster: G38 (= G38), C39 (= C39), G40 (= G40), G42 (= G42), C44 (= C44), G45 (= G45), C47 (= C47), C59 (= C59), C97 (= C97), C100 (= C100), Q101 (≠ T101), C132 (= C132), C134 (= C134)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q96 (= Q96), C134 (= C134)

1t3qA Crystal structure of quinoline 2-oxidoreductase from pseudomonas putida 86 (see paper)
45% identity, 91% coverage: 1:143/157 of query aligns to 4:147/162 of 1t3qA

query
sites
1t3qA

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binding fe2/s2 (inorganic) cluster: I40 (≠ E37), C42 (= C39), E43 (≠ G40), G45 (= G42), C47 (= C44), G48 (= G45), C50 (= C47), R60 (≠ L57), C62 (= C59), C101 (= C97), G102 (= G98), C104 (= C100), C136 (= C132), C138 (= C134)
binding pterin cytosine dinucleotide: Q100 (= Q96), C138 (= C134)

5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
47% identity, 94% coverage: 1:148/157 of query aligns to 3:152/157 of 5y6qA

query
sites
5y6qA

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binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), D42 (≠ G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C61 (= C59), C101 (= C97), G102 (= G98), C104 (= C100), C136 (= C132), C138 (= C134)
binding pterin cytosine dinucleotide: Q100 (= Q96), C138 (= C134)

1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
48% identity, 98% coverage: 3:156/157 of query aligns to 5:161/161 of 1sb3C

query
sites
1sb3C

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Q

V

V

Q

A

G

G

C

K

D

K

V

V

V

E

T

T

I

V

E

E

G

S

L

L

D

A

P

T

A

Q

G

G

H

T

-

L

H

S

P

K

V

L

Q

Q

E

A

A

A

F

F

T

H

G

E

S

K

G

L

A

G

V

T

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

M

I

I

M

M

S

A

L

S

S

E

T

A

Y

L

L

L

A

R

A

K

H

N

P

P

D

S

P

P

A

S

P

R

E

D

K

E

I

I

R

K

E

A

A

A

V

L

S

A

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

V

K

K

I

I

V

I

D

K

A

S

-

V

-

E

G

T

L

A

A

A

R

R

A

L

L

C

K

E

E

E

A

G

H

A

R

R

binding fe2/s2 (inorganic) cluster: Q39 (≠ E37), C41 (= C39), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C61 (= C59), C100 (= C97), G101 (= G98), C103 (= C100), C135 (= C132), C137 (= C134)
binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q96), C137 (= C134)

P19921 Carbon monoxide dehydrogenase small chain; CO dehydrogenase subunit S; CO-DH S; EC 1.2.5.3 from Afipia carboxidovorans (strain ATCC 49405 / DSM 1227 / KCTC 32145 / OM5) (Oligotropha carboxidovorans) (see 2 papers)
43% identity, 96% coverage: 3:152/157 of query aligns to 6:157/166 of P19921

query
sites
P19921

L

I

E

E

F

L

T

T

L

I

N

N

S

G

A

H

K

P

V

V

T

E

V

A

E

L

T

V

A

E

P

P

D

R

R

T

R

L

L

L

I

I

D

H

L

F

L

I

R

R

E

E

D

Q

L

Q

G

N

L

L

T

T

G

G

T

A

K

H

E

I

G

G

C
|
C

G

D

T

T

G

S

E

H

C
|
C

G

G

A

A

C
|
C

T

T

I

V

R

D

V

L

Q

D

G

G

E

M

T

S

K

V

L

K

A

S

C
|
C

L

T

M

M

L

F

A

A

A

V

Q

Q

V

A

Q

N

G

G

C

A

D

S

V

I

V

T

T

T

I

I

E

E

G

G

L

M

D

A

-

A

P

P

A

D

G

G

H

T

-

L

H

S

P

A

V

L

Q

Q

E

E

A

G

F

F

T

R

G

M

S

M

G

H

A

G

V

L

Q

Q

C
|
C

G

G

F

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

M

M

S

R

L

S

S

H

T

R

Y

L

L

L

A

Q

A

E

H

N

P

P

D

S

P

P

A

T

P

E

E

A

K

E

I

I

R

R

E

F

A

G

V

I

S

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

K

N

I

I

V

V

D

K

A

A

G

I

L

Q

A

Y

A

A

R

A

A

K

L

I

K

N

C42 (= C39) binding [2Fe-2S] cluster
C47 (= C44) binding [2Fe-2S] cluster
C50 (= C47) binding [2Fe-2S] cluster
C62 (= C59) binding [2Fe-2S] cluster
C102 (= C97) binding [2Fe-2S] cluster
C105 (= C100) binding [2Fe-2S] cluster
C137 (= C132) binding [2Fe-2S] cluster
C139 (= C134) binding [2Fe-2S] cluster

1n5wD Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
42% identity, 96% coverage: 2:152/157 of query aligns to 3:155/158 of 1n5wD

query
sites
1n5wD

K

H

L

I

E

E

F

L

T

T

L

I

N

N

S

G

A

H

K

P

V

V

T

E

V

A

E

L

T

V

A

E

P

P

D

R

R

T

R

L

L

L

I

I

D

H

L

F

L

I

R

R

E

E

D

Q

L

Q

G

N

L

L

T

T

G

G

T

A

K

H

E

I

G

G

C
|
C

G

D

T

T

G
x
S

E
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

R

D

V

L

Q

D

G

G

E

M

T

S

K

V

L

K

A

S

C
|
C

L

T

M

M

L

F

A

A

A

V

Q

Q

V

A

Q

N

G

G

C

A

D

S

V

I

V

T

T

T

I

I

E

E

G

G

L

M

D

A

-

A

P

P

A

D

G

G

H

T

-

L

H

S

P

A

V

L

Q

Q

E

E

A

G

F

F

T

R

G

M

S

M

G

H

A

G

V

L

Q
|
Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

M

M

S

R

L

S

S

H

T

R

Y

L

L

L

A

Q

A

E

H

N

P

P

D

S

P

P

A

T

P

E

E

A

K

E

I

I

R

R

E

F

A

G

V

I

S

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

K

N

I

I

V

V

D

K

A

A

G

I

L

Q

A

Y

A

A

R

A

A

K

L

I

K

N

binding flavin-adenine dinucleotide: S43 (≠ G42), H44 (≠ E43)
binding fe2/s2 (inorganic) cluster: C40 (= C39), S43 (≠ G42), C45 (= C44), G46 (= G45), C48 (= C47), C60 (= C59), C100 (= C97), G101 (= G98), C103 (= C100), C135 (= C132), C137 (= C134)
binding pterin cytosine dinucleotide: Q99 (= Q96), C137 (= C134)

1n5wA Crystal structure of the cu,mo-co dehydrogenase (codh); oxidized form (see paper)
43% identity, 96% coverage: 3:152/157 of query aligns to 4:155/161 of 1n5wA

query
sites
1n5wA

L

I

E

E

F

L

T

T

L

I

N

N

S

G

A

H

K

P

V

V

T

E

V

A

E

L

T

V

A

E

P

P

D

R

R

T

R

L

L

L

I

I

D

H

L

F

L

I

R

R

E

E

D

Q

L

Q

G

N

L

L

T

T

G

G

T

A

K

H

E
x
I

G
|
G

C
|
C

G

D

T

T

G
x
S

E
x
H

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

R

D

V

L

Q

D

G

G

E

M

T

S

K

V

L

K

A

S

C
|
C

L

T

M

M

L

F

A

A

A

V

Q

Q

V

A

Q

N

G

G

C

A

D

S

V

I

V

T

T

T

I

I

E

E

G

G

L

M

D

A

-

A

P

P

A

D

G

G

H

T

-

L

H

S

P

A

V

L

Q

Q

E

E

A

G

F

F

T

R

G

M

S

M

G

H

A

G

V

L

Q

Q

C
|
C

G
|
G

F

Y

C
|
C

T

T

P

P

G

G

M

M

I

I

M

M

S

R

L

S

S

H

T

R

Y

L

L

L

A

Q

A

E

H

N

P

P

D

S

P

P

A

T

P

E

E

A

K

E

I

I

R

R

E

F

A

G

V

I

S

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

Q

K

N

I

I

V

V

D

K

A

A

G

I

L

Q

A

Y

A

A

R

A

A

K

L

I

K

N

binding flavin-adenine dinucleotide: S43 (≠ G42), H44 (≠ E43)
binding fe2/s2 (inorganic) cluster: I38 (≠ E37), G39 (= G38), C40 (= C39), S43 (≠ G42), C45 (= C44), G46 (= G45), C48 (= C47), C60 (= C59), C100 (= C97), G101 (= G98), C103 (= C100), C135 (= C132), C137 (= C134)

3zyvB Crystal structure of the mouse liver aldehyde oxidase 3 (maox3) (see paper)
45% identity, 90% coverage: 2:143/157 of query aligns to 3:157/1262 of 3zyvB

query
sites
3zyvB

K

E

L

L

E

I

F

F

T

F

L

V

N

N

S

G

A

K

K

K

V

V

T

T

V

E

E

R

T

N

A

A

-

D

P

P

D

E

R

V

R

N

L

L

I

L

D

F

L

Y

L

L

R

R

E

K

D

V

L

I

G

R

L

L

T

T

G

G

T

T

K

K

E
x
Y

G

G

C
|
C

G
|
G

T

G

G
|
G

E

D

C
|
C

G

G

A

A

C
|
C

T

T

I

V

-

M

-

I

-

S

-

R

-

Y

-

D

-

P

-

I

-

S

-

K

R

R

V

I

Q

S

G

H

E

F

T

S

K

A

L
x
T

A

A

C
|
C

L

L

M

V

L

P

A

I

A

C

Q

S

V

L

Q

H

G

G

C

A

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

I

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

G

G

H

A

G

V

T

Q

Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

M

I

V

M

M

S

S

L

I

S

Y

T

T

Y

L

L

L

A

R

A

N

H

H

P

P

D

E

P

P

A

S

P

T

E

E

K

Q

I

I

R

M

E

E

A

T

V

L

S

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

V

D

E

A

S

binding fe2/s2 (inorganic) cluster: Y39 (≠ E37), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), C49 (= C47), T69 (≠ L57), C71 (= C59), C111 (= C97), G112 (= G98), C114 (= C100), C146 (= C132), C148 (= C134)

Sites not aligning to the query:

active site: 716, 751, 829, 833, 861, 1207, 1208
binding flavin-adenine dinucleotide: 227, 229, 230, 231, 232, 233, 234, 267, 303, 304, 312, 313, 316, 317, 319, 325, 326, 366, 392, 393

G3X982 Aldehyde oxidase 3; Aldehyde oxidase homolog 1; Azaheterocycle hydroxylase 3; EC 1.2.3.1; EC 1.17.3.- from Mus musculus (Mouse) (see paper)
44% identity, 91% coverage: 2:144/157 of query aligns to 9:164/1335 of G3X982

query
sites
G3X982

K

E

L

L

E

I

F

F

T

F

L

V

N

N

S

G

A

K

K

K

V

V

T

T

V

E

E

R

T

N

A

A

-

D

P

P

D

E

R

V

R

N

L

L

I

L

D

F

L

Y

L

L

R

R

E

K

D

V

L

I

G

R

L

L

T

T

G

G

T

T

K

K

E

Y

G

G

C
|
C

G

G

T

G

G

G

E

D

C
|
C

G

G

A

A

C
|
C

T

T

I

V

-

M

-

I

-

S

-

R

-

Y

-

D

-

P

-

I

-

S

-

K

R

R

V

I

Q

S

G

H

E

F

T

S

K

A

L

T

A

A

C
|
C

L

L

M

V

L

P

A

I

A

C

Q

S

V

L

Q

H

G

G

C

A

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

I

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

G

G

H

A

G

V

T

Q
|
Q

C
|
C

G

G

F

F

C
|
C

T

T

P

P

G

G

M

M

I

V

M

M

S

S

L

I

S

Y

T

T

Y

L

L

L

A

R

A

N

H

H

P

P

D

E

P

P

A

S

P

T

E

E

K

Q

I

I

R

M

E

E

A

T

V

L

S

G

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

V

D

E

A

S

G

A

C47 (= C39) binding [2Fe-2S] cluster
C52 (= C44) binding [2Fe-2S] cluster
C55 (= C47) binding [2Fe-2S] cluster
C77 (= C59) binding [2Fe-2S] cluster
Q116 (= Q96) binding Mo-molybdopterin
C117 (= C97) binding [2Fe-2S] cluster
C120 (= C100) binding [2Fe-2S] cluster
C152 (= C132) binding [2Fe-2S] cluster
C154 (= C134) binding [2Fe-2S] cluster

Sites not aligning to the query:

264:271 binding FAD
354 binding FAD
358 binding FAD
367 binding FAD
411 binding FAD
802 binding Mo-molybdopterin
807 A→V: No effect on kinetic constants with smaller substrates like benzaldehyde or phthalazine. Decreases substrate affinity and slightly increases catalytic efficiency for bulkier substrates like phenanthridine.
885 Y→M: Slightly decreases substrate affinity but no effect on activity with smaller substrates like benzaldehyde or phthalazine. Increases catalytic efficiency with bulkier substrates like phenanthridine or more charged substrates like N1-methylnicotinamide.
889 K→H: No effect on substrate affinity but decreases catalytic efficiency for smaller substrates like benzaldehyde or phthalazine. Increases substrate affinity and activity for bulkier substrates like phenanthridine.
1043 binding Mo-molybdopterin
1199 binding Mo-molybdopterin
1266 E→Q: Loss of activity with different N-heterocyclic compounds as substrates. 60% reduction of activity with benzaldehyde.

Q8GUQ8 Xanthine dehydrogenase 1; AtXDH1; EC 1.17.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
50% identity, 78% coverage: 22:143/157 of query aligns to 37:170/1361 of Q8GUQ8

query
sites
Q8GUQ8

L

L

I

L

D

E

L

Y

L

L

R

R

E

-

D

D

L

L

G

G

L

L

T

T

G

G

T

T

K

K

E

L

G

G

C

C

G

G

T

E

G

G

E

G

C

C

G

G

A

A

C

C

T

T

I

V

R

M

V

V

-

S

-

Y

-

D

-

R

Q

K

G

S

E

K

T

T

K

S

L

V

-

H

-

Y

-

A

-

V

-

N

A

A

C

C

L

L

M

A

L

P

A

L

A

Y

Q

S

V

V

Q

E

G

G

C

M

D

H

V

V

V

I

T

S

I

I

E

E

G

G

L

L

D

G

-

H

-

R

P

K

A

L

G

G

H

L

H

H

P

P

V

V

Q

Q

E

E

A

S

F

L

T

A

G

S

S

S

G

H

A

G

V

S

Q

Q

C

C

G

G

F

F

C

C

T

T

P

P

G

G

M

F

I

I

M

M

S

S

L

M

S

Y

T

S

Y

L

L

L

-

R

A

S

H

K

P

N

D

S

P

P

A

S

P

E

E

E

K

E

I

I

R

E

E

E

A

C

V

L

S

A

G

G

N

N

L

L

C

C

R

R

C

C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

V

D

D

A

A

Sites not aligning to the query:

364 W→A: Decreases activity 8-fold.
421 Y→A: Decreases activity 4-fold.
831 E→A: Loss of activity.
909 R→A: Decreases activity 12-fold.
1297 E→A: Decreases activity 40-fold.

2ckjA Human milk xanthine oxidoreductase
43% identity, 94% coverage: 2:149/157 of query aligns to 3:163/1264 of 2ckjA

query
sites
2ckjA

K

K

L

L

E

V

F

F

T

F

L

V

N

N

S

G

A

R

K

K

V

V

T

V

V

E

E

K

T

N

A

A

-

D

P

P

D

E

R

T

L

L

I

L

D

A

L

Y

L

L

R

R

E

R

D

K

L

L

G

G

L

L

T

S

G

G

T

T

K

K

E

L

G
|
G

C
|
C

G
|
G

T

E

G
|
G

E

G

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

R

M

V

L

Q

S

G

K

E

Y

T

D

K

R

L

L

-

Q

-

N

-

K

-

I

-

V

-

H

-

F

-

S

-

A

-

N

A

A

C
|
C

L

L

M

A

L

P

A

I

A

C

Q

S

V

L

Q

H

G

H

C

V

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

L

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

S

G

H

A

G

V

S

Q

Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

I

I

V

M

M

S

S

L

M

S

Y

T

T

Y

L

L

L

A

R

A

N

H

Q

P

P

D

E

P

P

A

T

P

M

E

E

K

E

I

I

R

E

E

N

A

A

V

F

S

Q

G

G

N

N

L

L

C
|
C

R
|
R

C
|
C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

L

D

Q

A

G

G

F

L

R

A

T

A

F

A

A

R

R

binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), C111 (= C97), G112 (= G98), C114 (= C100), C146 (= C132), R147 (= R133), C148 (= C134)

Sites not aligning to the query:

active site: 711, 746, 824, 828, 856, 1204, 1205
binding flavin-adenine dinucleotide: 228, 230, 231, 232, 233, 234, 308, 309, 317, 318, 321, 322, 324, 325, 331, 375

2e1qA Crystal structure of human xanthine oxidoreductase mutant, glu803val (see paper)
43% identity, 94% coverage: 2:149/157 of query aligns to 3:163/1307 of 2e1qA

query
sites
2e1qA

K

K

L

L

E

V

F

F

T

F

L

V

N

N

S

G

A

R

K

K

V

V

T

V

V

E

E

K

T

N

A

A

-

D

P

P

D

E

R

T

L

L

I

L

D

A

L

Y

L

L

R

R

E

R

D

K

L

L

G

G

L

L

T

S

G

G

T

T

K

K

E

L

G
|
G

C
|
C

G
|
G

T
x
E

G
|
G

E

G

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

R

M

V

L

Q

S

G

K

E

Y

T

D

K

R

L

L

-

Q

-

N

-

K

-

I

-

V

-

H

-

F

-

S

-

A

-

N

A

A

C
|
C

L

L

M

A

L

P

A

I

A

C

Q

S

V

L

Q

H

G

H

C

V

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

L

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

S

G

H

A

G

V

S

Q
|
Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

I

I

V

M

M

S

S

L

M

S

Y

T

T

Y

L

L

L

A

R

A

N

H

Q

P

P

D

E

P

P

A

T

P

M

E

E

K

E

I

I

R

E

E

N

A

A

V

F

S

Q

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

L

D

Q

A

G

G

F

L

R

A

T

A

F

A

A

R

R

binding flavin-adenine dinucleotide: E43 (≠ T41), G44 (= G42)
binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), Q110 (= Q96), C111 (= C97), G112 (= G98), C114 (= C100), C146 (= C132), C148 (= C134)

Sites not aligning to the query:

active site: 742, 777, 855, 859, 887, 1235, 1236
binding bicarbonate ion: 814, 815, 852, 886, 889, 890, 893
binding calcium ion: 715, 716, 718, 741, 747, 780, 781, 784, 811, 812
binding flavin-adenine dinucleotide: 230, 231, 232, 233, 234, 235, 236, 237, 238, 311, 312, 316, 320, 321, 324, 325, 327, 328, 333, 334, 377, 378
binding fe2/s2 (inorganic) cluster: 719
binding hydroxy(dioxo)molybdenum: 773, 774, 887, 1053, 1054, 1236

P47989 Xanthine dehydrogenase/oxidase; EC 1.17.1.4; EC 1.17.3.2 from Homo sapiens (Human) (see 4 papers)
43% identity, 94% coverage: 2:149/157 of query aligns to 5:165/1333 of P47989

query
sites
P47989

K

K

L

L

E

V

F

F

T

F

L

V

N

N

S

G

A

R

K

K

V

V

T

V

V

E

E

K

T

N

A

A

-

D

P

P

D

E

R

T

L

L

I

L

D

A

L

Y

L

L

R

R

E

R

D

K

L

L

G

G

L

L

T

S

G

G

T

T

K

K

E

L

G

G

C

C

G

G

T

E

G

G

E

G

C

C

G

G

A

A

C

C

T

T

I

V

R

M

V

L

Q

S

G

K

E

Y

T

D

K

R

L

L

-

Q

-

N

-

K

-

I

-

V

-

H

-

F

-

S

-

A

-

N

A

A

C

C

L

L

M

A

L

P

A

I

A

C

Q

S

V

L

Q

H

G

H

C

V

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

L

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

S

G

H

A

G

V

S

Q

Q

C

C

G

G

F

F

C

C

T

T

P

P

G

G

M

I

I

V

M

M

S

S

L

M

S

Y

T

T

Y

L

L

L

A

R

A

N

H

Q

P

P

D
x
E

P

P

A

T

P

M

E

E

K

E

I

I

R

E

E

N

A

A

V

F

S

Q

G

G

N

N

L

L

C

C

R

R

C

C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

L

D

Q

A

G

G

F

L

R

A

T

A

F

A

A

R

R

E133 (≠ D117) to K: in dbSNP:rs45447191

Sites not aligning to the query:

172 G → R: in dbSNP:rs45523133
235 T → M: in dbSNP:rs45469499
257:264 binding FAD
337 binding FAD
347:351 binding FAD
360 binding FAD
395 K → M: in dbSNP:rs34929837
422 binding FAD
509 modified: Disulfide link with 1318, In oxidase form
536 modified: Disulfide link with 993, In oxidase form
555 P → S: in dbSNP:rs45577338
584 D → A: in dbSNP:rs45491693
607 R → Q: in dbSNP:rs45442092
617 K → N: in dbSNP:rs45442398
623 T → I: in dbSNP:rs45448694
646 I → V: in dbSNP:rs17323225
703 I → V: in dbSNP:rs17011368
763 L → F: in a breast cancer sample; somatic mutation
791 R → G: in a breast cancer sample; somatic mutation; dbSNP:rs775646772
910 T → M: in dbSNP:rs669884
993 modified: Disulfide link with 536, In oxidase form
1091 V → L: in dbSNP:rs45619033
1109 N → T: in dbSNP:rs45547640
1150 P → R: in dbSNP:rs1042036
1176 R → C: in dbSNP:rs45624433
1296 R → W: in dbSNP:rs45564939
1318 modified: Disulfide link with 509, In oxidase form

3am9A Complex of bovine xanthine dehydrogenase and trihydroxy fyx-051 (see paper)
41% identity, 95% coverage: 2:150/157 of query aligns to 3:164/1293 of 3am9A

query
sites
3am9A

K

E

L

L

E

V

F

F

T

F

L

V

N

N

S

G

A

K

K

K

V

V

T

V

V

E

E

K

T

N

A

A

-

D

P

P

D

E

R

T

L

L

I

L

D

A

L

Y

L

L

R

R

E

R

D

K

L

L

G

G

L

L

T

R

G

G

T

T

K

K

E

L

G
|
G

C
|
C

G
|
G

T
x
E

G
|
G

E

G

C
|
C

G
|
G

A

A

C
|
C

T

T

I

V

R

M

V

L

Q

S

G

K

E

Y

T

D

K

R

L

L

-

Q

-

D

-

K

-

I

-

H

-

F

-

S

-

A

-

N

A

A

C
|
C

L

L

M

A

L

P

A

I

A

C

Q

T

V

L

Q

H

G

H

C

V

D

A

V

V

V

T

T

T

I

V

E

E

G

G

L

I

D

G

P

S

A

T

G

K

H

T

-

R

-

L

H

H

P

P

V

V

Q

Q

E

E

A

R

F

I

T

A

G

K

S

S

G

H

A

G

V

S

Q

Q

C
|
C

G
|
G

F

F

C
|
C

T

T

P

P

G

G

M

I

I

V

M

M

S

S

L

M

S

Y

T

T

Y

L

L

L

A

R

A

N

H

Q

P

P

D

E

P

P

A

T

P

V

E

E

K

E

I

I

R

E

E

D

A

A

V

F

S

Q

G

G

N

N

L

L

C
|
C

R

R

C
|
C

T

T

G

G

Y

Y

Q

R

K

P

I

I

V

L

D

Q

A

G

G

F

L

R

A

T

A

F

A

A

R

K

A

S

binding flavin-adenine dinucleotide: E43 (≠ T41), G44 (= G42)
binding fe2/s2 (inorganic) cluster: G40 (= G38), C41 (= C39), G42 (= G40), G44 (= G42), C46 (= C44), G47 (= G45), C49 (= C47), C71 (= C59), C111 (= C97), G112 (= G98), C114 (= C100), C146 (= C132), C148 (= C134)

Sites not aligning to the query:

active site: 733, 768, 846, 850, 878, 1226, 1227
binding bicarbonate ion: 805, 806, 875, 876, 877, 884
binding calcium ion: 707, 709, 802, 803, 833, 836, 837, 840, 873, 874
binding flavin-adenine dinucleotide: 228, 229, 231, 232, 233, 234, 235, 236, 309, 310, 314, 318, 319, 322, 323, 325, 326, 332, 376, 394
binding fe2/s2 (inorganic) cluster: 710
binding 4-[5-(2,6-dioxo-1,2,3,6-tetrahydropyridin-4-yl)-1H-1,2,4-triazol-3-yl]-6-oxo-1,6-dihydropyridine-2-carbonitrile: 614, 768, 839, 842, 846, 880, 975, 980, 1045, 1227

Query Sequence

>WP_035235863.1 NCBI__GCF_000745975.1:WP_035235863.1
MKLEFTLNSAKVTVETAPDRRLIDLLREDLGLTGTKEGCGTGECGACTIRVQGETKLACL
MLAAQVQGCDVVTIEGLDPAGHHPVQEAFTGSGAVQCGFCTPGMIMSLSTYLAAHPDPAP
EKIREAVSGNLCRCTGYQKIVDAGLAAARALKEAHRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory