SitesBLAST

P11349 Respiratory nitrate reductase 1 beta chain; Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta; EC 1.7.5.1 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 34% coverage: 85:132/143 of query aligns to 183:232/512 of P11349

query
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P11349

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C184 (= C86) binding
C187 (= C89) binding
C192 (= C92) binding
C196 (= C96) binding
C217 (= C117) binding
C223 (= C123) binding
C227 (= C127) binding

Sites not aligning to the query:

16 binding
19 binding
22 binding
26 binding
244 binding
247 binding
259 binding
263 binding

7b04A of Nitrite oxidoreductase (Nxr) from the anammox bacterium Kuenenia stuttgartiensis.
25% identity, 72% coverage: 28:130/143 of query aligns to 113:220/409 of 7b04A

query
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7b04A

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binding fe3-s4 cluster: C186 (= C96), I191 (≠ L101), C207 (= C117), R208 (≠ S118), G209 (≠ L119), Y210 (≠ C120), R211 (≠ E121), K212 (≠ R122), C213 (= C123)
binding protoporphyrin ix containing fe: R188 (≠ T98), R208 (≠ S118), Y210 (≠ C120)
binding iron/sulfur cluster: Q171 (≠ E83), I173 (= I85), C174 (= C86), N175 (≠ T87), H176 (= H88), C177 (= C89), P180 (vs. gap), C182 (= C92), C217 (= C127)

Sites not aligning to the query:

binding fe3-s4 cluster: 231
binding iron/sulfur cluster: 34, 35, 36, 37, 38, 40, 44, 48, 59, 60, 62, 222, 233, 234, 236, 237, 255, 256, 257, 261, 262, 265, 266

6cfwN Cryoem structure of a respiratory membrane-bound hydrogenase (see paper)
29% identity, 63% coverage: 50:139/143 of query aligns to 6:98/121 of 6cfwN

query
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binding iron/sulfur cluster: C45 (= C86), G47 (≠ H88), C48 (= C89), M50 (≠ A91), C51 (= C92), C55 (= C96), C76 (= C117), M78 (≠ L119), C79 (= C120), C82 (= C123), C86 (= C127)

Query Sequence

>WP_035236918.1 NCBI__GCF_000745975.1:WP_035236918.1
MYSKIVILDFPPQSAQRPIVCELVKKYDLMFNILKARISSRSEGHLVLEISSAGKSAFNK
GITYLKEQGVRVSTPEHKIYKDEEICTHCGACTAVCPTGALYIKRPEMEVIFDREKCSLC
ERCLLTCPTRAMGLFSEDLKETV

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory