SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035237565.1 NCBI__GCF_000745975.1:WP_035237565.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
45% identity, 87% coverage: 15:263/285 of query aligns to 11:276/304 of Q96XT4

query
sites
Q96XT4

W

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C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
IGCS 44:47 (≠ IGQA 48:51) binding thiamine diphosphate
C46 (≠ Q50) binding [4Fe-4S] cluster
D90 (= D92) binding Mg(2+)
GD 91:92 (= GD 93:94) binding thiamine diphosphate
N118 (= N120) binding Mg(2+)
V120 (≠ I122) binding Mg(2+)
GL 122:123 (= GL 124:125) binding thiamine diphosphate
C197 (= C199) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
45% identity, 87% coverage: 15:263/285 of query aligns to 8:273/301 of 5b46B

query
sites
5b46B

W
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binding magnesium ion: D87 (= D92), N115 (= N120), V117 (≠ I122)
binding iron/sulfur cluster: W8 (= W15), C9 (= C16), C12 (= C19), C43 (≠ Q50), C194 (= C199), T196 (≠ S201), Y197 (≠ F202)
binding thiamine diphosphate: I41 (= I48), G42 (= G49), C43 (≠ Q50), S44 (≠ A51), H62 (= H67), G86 (= G91), G88 (= G93), D89 (= D94), N115 (= N120), V117 (≠ I122), Y118 (= Y123), G119 (= G124), L120 (= L125), T121 (= T126)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
43% identity, 91% coverage: 13:272/285 of query aligns to 9:278/305 of Q96Y68

query
sites
Q96Y68

N

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C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
C46 (≠ Q50) binding [4Fe-4S] cluster
K49 (= K53) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D92) binding Mg(2+)
GD 91:92 (= GD 93:94) binding thiamine diphosphate
N118 (= N120) binding Mg(2+)
V120 (≠ I122) binding Mg(2+)
GL 122:123 (= GL 124:125) binding thiamine diphosphate
K125 (= K127) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ G175) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C199) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
43% identity, 91% coverage: 13:272/285 of query aligns to 9:278/305 of P72579

query
sites
P72579

N

N

K

D

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W

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K49 (= K53) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L125) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
43% identity, 91% coverage: 13:272/285 of query aligns to 5:260/286 of 5b48B

query
sites
5b48B

N

N

K

D

W

W

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M

G

F

V

H

H

G

T

L

L

H
|
H

G

G

R

R

A

A

L

I

P

A

L

F

A

A

T

T

G

G

T

I

K

K

I

L

A

S

N

N

N

P

D

D

L

L

T

V

V

V

V

I

V

V

N

N

C

G

G

G

D
|
D

G
|
G

D
|
D

C

L

Y

L

G

G

E

I

G

G

G

A

N

G

H

H

F

F

L

V

A

A

I

G

R

R

N

N

I

V

D

D

M

M

T

V

L

V

L

I

V

L

H

H

N

D

N
|
N

Q

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

A

A

S

S

P

P

T

T

S

-

L

-

G

-

M

-

V

-

T

-

K

-

L

L

Q

K

N

R

S

G

G

E

T

N

P

I

S

N

S

D

Q

A

F

V

S

N

A

P

L

I

V

A

I

L

A

A

L

I

A

S

G

G

A

Y

G

T

F

F

V

V

A

A

R

R

G

G

L

Y

S

A

A

Y

E

D

S

V

G

K

H

H

L

L

T

K

E

E

L

L

I

I

V

K

K

S

A

A

M

I

N

K

Y

H

K

K

G

G

F

L

A

A

L

L

V

I

D

D

I

V

L

L

Q

Q

P

P

C
|
C

V

P

S
x
T

F

Y

N

N

K

D

I

I

N

N

T

T

L

K

S

E

W

W

Y

-

K

-

E

-

R

R

A

I

Y

Y

K

K

L

L

A

-

D

D

T

T

-

L

-

P

D

D

H

W

D

D

P

P

-

V

-

K

-

P

-

E

-

V

-

N

R

E

D

K

L

I

A

K

K

R

A

A

F

I

N

D

L

K

A

S

Q

L

E

E

W

W

E

-

K

G

S

D

L

I

P

P

L

I

G

G

V

I

L

F

Y

Y

E

Q

A

N

A

E

G

L

T

V

P

P

F

S

H

Y

E

E

R

E

V

-

P

-

N

R

L

I

K

K

A

A

N

N

A

S

L

P

A

A

S

Y

H

L

N

D

Y

Y

binding magnesium ion: D84 (= D92), N112 (= N120), V114 (≠ I122)
binding iron/sulfur cluster: C8 (= C16), C11 (= C19), C42 (≠ Q50), C183 (= C199), T185 (≠ S201)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I48), G41 (= G49), C42 (≠ Q50), S43 (≠ A51), H59 (= H67), G85 (= G93), D86 (= D94), N112 (= N120), V114 (≠ I122), Y115 (= Y123), G116 (= G124), L117 (= L125)

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
37% identity, 95% coverage: 2:273/285 of query aligns to 11:281/291 of 6n2oD

query
sites
6n2oD

I

M

T

K

S

P

K

K

E

D

F

Y

N

K

C

S

N

E

Y

V

E

P

N

T

K

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N

H

F

F

Q

G

I

I

L

L

A

N

A

G

M

V

K

Y

N

R

A

A

F

M

A

A

Q

E

Q

L

Q

G

I

I

P

D

P

S

E

T

K

K

L

F

T

A

L

A

I

I

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

G

S

K
x
R

T

M

P

P

H

Y

F

F

L

V

K

D

C

S

N

Y

M

K

F

M

H

H

G

T

L

L

H
|
H

G

G

R

R

A

A

L

G

P

A

L

V

A

A

T

T

G

G

T

T

K

Q

I

V

A

A

N

R

N

P

D

D

L

L

T

C

V

V

N

A

C

G

G

G

D
|
D

G
|
G

D
|
D

C

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

I

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

N

D

N
|
N

Q

G

I
|
I

Y

Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

S

S

P

P

T

T

S

S

S

R

L

P

G

E

M

M

V

T

T

A

K

Y

L

T

Q

T

N

P

S

Y

G

G

T

G

P

P

S

E

S

N

Q

P

F

M

S

N

A

P

L

L

V

L

I

Y

A

M

L

L

A

T

A

Y

G

G

A

A

G

T

F

Y

V

V

A

A

R

Q

G

A

L

F

S

A

A

G

E

K

S

P

G

K

H

D

L

C

T

A

E

E

L

L

I

I

V

K

K

G

A

A

M

M

N

E

Y

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

I

I

L

F

Q

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

I

I

N

D

T

T

L

V

S

D

W

F

Y

Y

K

R

E

D

R

L

A

V

Y

E

K

P

L

I

A

P

D

E

T

-

D

D

H

H

D

D

P

T

R

S

D

D

L

L

A

G

K

A

A

A

F

M

N

E

L

L

A

A

Q

R

E

R

W

P

E

G

K

G

S

K

L

A

P

P

L

T

G

G

V

L

L

L

Y

Y

E

K

A

T

A

S

G

A

T

P

P

T

F

L

H

D

E

Q

R

N

V

L

P

A

N

K

L

I

K

R

A

E

N

R

A

L

L

G

A

G

S

H

H

V

N

G

Y

Y

D

D

binding magnesium ion: D101 (= D92), N129 (= N120), I131 (= I122)
binding succinyl-coenzyme a: I57 (= I48), R62 (≠ K53), L134 (= L125), K136 (= K127)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (≠ Q50), C208 (= C199), T210 (≠ S201), F211 (= F202)
binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (≠ Q50), S60 (≠ A51), H76 (= H67), G102 (= G93), D103 (= D94), N129 (= N120), I131 (= I122), G133 (= G124), L134 (= L125), T135 (= T126)

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
37% identity, 95% coverage: 2:273/285 of query aligns to 11:281/291 of 6n2oB

query
sites
6n2oB

I

M

T

K

S

P

K

K

E

D

F

Y

N

K

C

S

N

E

Y

V

E

P

N

T

K

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N

H

F

F

Q

G

I

I

L

L

A

N

A

G

M

V

K

Y

N

R

A

A

F

M

A

A

Q

E

Q

L

Q

G

I

I

P

D

P

S

E

T

K

K

L

F

T

A

L

A

I

I

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

G

S

K
x
R

T

M

P

P

H

Y

F

F

L

V

K

D

C

S

N

Y

M

K

F

M

H

H

G

T

L

L

H
|
H

G

G

R

R

A

A

L

G

P

A

L

V

A

A

T

T

G

G

T

T

K

Q

I

V

A

A

N

R

N

P

D

D

L

L

T

C

V

V

N

A

C

G

G

G

D
|
D

G
|
G

D
|
D

C

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

I

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

N

D

N
|
N

Q

G

I
|
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K
|
K

G

G

Q

Q

A

Y

S

S

P

P

T

T

S

S

S

R

L

P

G

E

M

M

V

T

T

A

K
x
Y

L

T

Q

T

N

P

S

Y

G

G

T

G

P

P

S

E

S

N

Q

P

F

M

S

N

A

P

L

L

V

L

I

Y

A

M

L

L

A

T

A

Y

G

G

A

A

G

T

F

Y

V

V

A

A

R

Q

G

A

L

F

S

A

A

G

E

K

S

P

G

K

H

D

L

C

T

A

E

E

L

L

I

I

V

K

K

G

A

A

M

M

N

E

Y

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

I

I

L

F

Q

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

I

I

N

D

T

T

L

V

S

D

W

F

Y

Y

K

R

E

D

R

L

A

V

Y

E

K

P

L

I

A

P

D

E

T

-

D

D

H

H

D

D

P

T

R

S

D

D

L

L

A

G

K

A

A

A

F

M

N

E

L

L

A

A

Q

R

E

R

W

P

E

G

K

G

S

K

L

A

P

P

L

T

G

G

V

L

L

L

Y

Y

E

K

A

T

A

S

G

A

T

P

P

T

F

L

H

D

E

Q

R

N

V

L

P

A

N

K

L

I

K

R

A

E

N

R

A

L

L

G

A

G

S

H

H

V

N

G

Y

Y

D

D

binding 2-oxoglutaric acid: R62 (≠ K53), L134 (= L125)
binding coenzyme a: K136 (= K127), Y150 (≠ K141)
binding magnesium ion: D101 (= D92), N129 (= N120), I131 (= I122)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (≠ Q50), C208 (= C199), T210 (≠ S201), F211 (= F202)
binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (≠ Q50), S60 (≠ A51), H76 (= H67), G102 (= G93), D103 (= D94), N129 (= N120), I131 (= I122), Y132 (= Y123), G133 (= G124), L134 (= L125), T135 (= T126)

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
37% identity, 95% coverage: 2:273/285 of query aligns to 11:281/291 of 6n2nB

query
sites
6n2nB

I

M

T

K

S

P

K

K

E

D

F

Y

N

K

C

S

N

E

Y

V

E

P

N

T

K

T

W
|
W

C
|
C

P

P

G

G

C
|
C

G

G

N
x
H

F

F

Q

G

I

I

L

L

A

N

A

G

M

V

K

Y

N

R

A

A

F

M

A

A

Q

E

Q

L

Q

G

I

I

P

D

P

S

E

T

K

K

L

F

T

A

L

A

I

I

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

G

S

K

R

T

M

P

P

H

Y

F

F

L

V

K

D

C

S

N

Y

M

K

F

M

H

H

G

T

L

L

H
|
H

G

G

R

R

A

A

L

G

P

A

L

V

A

A

T

T

G

G

T

T

K

Q

I

V

A

A

N

R

N

P

D

D

L

L

T

C

V

V

N

A

C

G

G
|
G

D
|
D

G
|
G

D
|
D

C

G

Y

F

G

S

E

I

G

G

N

G

H

H

F

M

L

P

A

H

A

M

I

A

R

R

R

K

N

N

I

V

D

N

M

M

T

T

L

Y

L

V

V

L

H

M

N

D

N
|
N

Q

G

I
|
I

Y
|
Y

G
|
G

L
|
L

T
|
T

K

K

G

G

Q

Q

A

Y

S

S

P

P

T

T

S

S

S

R

L

P

G

E

M

M

V

T

T

A

K

Y

L

T

Q

T

N

P

S

Y

G

G

T

G

P

P

S

E

S

N

Q

P

F

M

S

N

A

P

L

L

V

L

I

Y

A

M

L

L

A

T

A

Y

G

G

A

A

G

T

F

Y

V

V

A

A

R

Q

G

A

L

F

S

A

A

G

E

K

S

P

G

K

H

D

L

C

T

A

E

E

L

L

I

I

V

K

K

G

A

A

M

M

N

E

Y

H

K

E

G

G

F

F

A

A

L

Y

V

V

D

N

I

I

L

F

Q

S

P

Q

C
|
C

V

P

S
x
T

F
|
F

N

N

K

K

I

I

N

D

T

T

L

V

S

D

W

F

Y

Y

K

R

E

D

R

L

A

V

Y

E

K

P

L

I

A

P

D

E

T

-

D

D

H

H

D

D

P

T

R

S

D

D

L

L

A

G

K

A

A

A

F

M

N

E

L

L

A

A

Q

R

E

R

W

P

E

G

K

G

S

K

L

A

P

P

L

T

G

G

V

L

L

L

Y

Y

E

K

A

T

A

S

G

A

T

P

P

T

F

L

H

D

E

Q

R

N

V

L

P

A

N

K

L

I

K

R

A

E

N

R

A

L

L

G

A

G

S

H

H

V

N

G

Y

Y

D

D

binding magnesium ion: D101 (= D92), N129 (= N120), I131 (= I122)
binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), H30 (≠ N21), C59 (≠ Q50), C208 (= C199), T210 (≠ S201), F211 (= F202)
binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (≠ Q50), S60 (≠ A51), H76 (= H67), G100 (= G91), D101 (= D92), G102 (= G93), D103 (= D94), N129 (= N120), I131 (= I122), Y132 (= Y123), G133 (= G124), L134 (= L125), T135 (= T126)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
41% identity, 87% coverage: 15:263/285 of query aligns to 4:247/275 of 5b47B

query
sites
5b47B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

N

D

F

F

Q

G

I

I

L

L

A

R

A

A

M

E

K

E

N

M

A

A

F

I

A

R

Q

E

Q

L

Q

G

I

I

P

N

P

P

E

K

K

S

L

V

T

V

L

I

I

V

S

S

G

G

I
|
I

G
|
G

Q
x
C

A
x
S

G

G

K

K

T

I

P

P

H

H

F

F

L

M

K

N

C

L

N

P

M

I

-

S

-

G

F

V

H

H

G

T

L

L

H
|
H

G

G

R

R

A

S

L

I

P

A

L

F

A

A

T

T

G

G

T

I

K

K

I

L

A

S

N

N

N

P

D

S

L

L

T

E

V

V

V

I

V

V

N

N

C

V

G

G

D
|
D

G
|
G

D
|
D

C

G

Y

L

G

G

E

I

G

G

G

M

N

G

H

H

F

F

L

V

A

H

A

L

I

G

R

R

N

N

I

I

D

D

M

I

T

A

L

V

L

L

V

V

H

H

N

N

N
|
N

Q

G

I
x
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

A

A

S

S

P

P

T

T

S

L

S

H

L

R

G

G

M

-

V

-

T

E

K

N

L

I

Q

M

N

D

S

A

G

V

T

N

P

P

S

-

Q

-

F

-

S

-

A

-

L

L

V

A

I

V

A

A

L

L

A

A

G

G

A

Y

G

T

F

F

V

V

A

A

R

R

G

G

L

Y

S

A

A

Y

E

D

S

V

G

M

H

H

L

L

T

K

E

E

L

L

I

I

V

K

K

K

A

A

M

I

N

L

Y

H

K

K

G

G

F

S

A

A

L

L

V

V

D

D

I

I

L

L

Q

Q

P

P

C
|
C

V

P

S

T

F

Y

N

R

-

V

-

Y

K

K

I

L

N

D

T

N

L

V

S

P

W

G

Y

W

K

D

E

P

R

V

A

V

Y

R

K

K

L

E

A

E

D

E

T

A

D

Q

H

-

D

-

P

-

R

K

D

K

L

F

A

E

K

Q

A

A

F

I

N

M

L

K

A

S

Q

Y

E

E

W

W

E

G

K

E

S

K

L

I

P

P

L

I

G

G

V

I

L

F

Y

Y

E

Q

A

N

A

E

G

L

T

V

P

P

-

T

F

F

H

E

E

D

R

R

-

L

-

T

-

S

-

N

V

I

P

P

N

N

L

Y

K

R

binding magnesium ion: D83 (= D92), N111 (= N120)
binding iron/sulfur cluster: C5 (= C16), C8 (= C19), C39 (≠ Q50), C179 (= C199)
binding thiamine diphosphate: I37 (= I48), G38 (= G49), C39 (≠ Q50), S40 (≠ A51), H58 (= H67), G84 (= G93), D85 (= D94), N111 (= N120), V113 (≠ I122), Y114 (= Y123), L116 (= L125)

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
27% identity, 47% coverage: 91:225/285 of query aligns to 966:1099/1171 of Q2RMD6

query
sites
Q2RMD6

G

G

D
|
D

G
|
G

D
x
W

C

A

Y

Y

-

D

-

I

G

G

E

Y

G

G

-

L

N

D

H

H

F

V

L

L

A

A

A

S

I

-

R

-

N

G

I

A

D

N

M

V

T

N

L

V

L

L

V

V

H

L

N

D

N
x
T

Q
x
E

I
x
V

Y
|
Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

S

T

S

Q

L

T

G

G

M

A

V

V

T

A

K

R

L

F

Q

A

N

A

S

G

G

G

T

K

P

F

S

T

S

K

Q

K

F

K

S

D

A

L

L

G

V

L

I

M

A

A

L

M

A

S

A

Y

G

G

A

Y

G

V

F

Y

V

V

A

A

R

S

-

V

G

A

L

M

S

G

A

A

E

S

S

H

G

S

H

Q

L

L

T

M

E

K

L

A

I

L

V

I

K

E

A

A

M

E

N

K

Y

Y

K

D

G

G

F

P

A

S

L

L

V

I

D

I

I

A

L

Y

Q

A

P

P

C
|
C

V

I

S

N

F

-

N

H

K

G

I

I

N

N

T

-

L

M

S

T

W

Y

Y

S

K

Q

E

R

R

E

A

A

Y

K

K

K

L

A

A

V

D

E

T

A

D

G

H

Y

D

W

P

P

D967 (= D92) binding Mg(2+)
DGW 967:969 (≠ DGD 92:94) binding thiamine diphosphate
T995 (≠ N120) binding Mg(2+)
TEVYSN 995:1000 (≠ NQIYGL 120:125) binding thiamine diphosphate
V997 (≠ I122) binding Mg(2+)
C1075 (= C199) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
27% identity, 47% coverage: 91:225/285 of query aligns to 965:1098/1164 of 6cioA

query
sites
6cioA

G
|
G

D
|
D

G
|
G

D

W

C

A

Y

Y

-

D

-

I

G

G

E

Y

G

G

-

L

N

D

H

H

F

V

L

L

A

A

A

S

I

-

R

-

N

G

I

A

D

N

M

V

T

N

L

V

L

L

V

V

H

L

N

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

S

T

S

Q

L

T

G

G

M

A

V

V

T

A

K

R

L

F

Q

A

N

A

S

G

G

G

T

K

P

F

S

T

S

K

Q

K

F

K

S

D

A

L

L

G

V

L

I

M

A

A

L

M

A

S

A

Y

G

G

A

Y

G

V

F

Y

V

V

A

A

R

S

-

V

G

A

L

M

S

G

A

A

E

S

S

H

G

S

H

Q

L

L

T

M

E

K

L

A

I

L

V

I

K

E

A

A

M

E

N

K

Y

Y

K

D

G

G

F

P

A

S

L

L

V

I

D

I

I

A

L

Y

Q

A

P

P

C
|
C

V
x
I

S

N

F

-

N

H

K

G

I

I

N

N

T

-

L

M

S

T

W

Y

Y

S

K

Q

E

R

R

E

A

A

Y

K

K

K

L

A

A

V

D

E

T

A

D

G

H

Y

D

W

P

P

active site: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: C1074 (= C199), I1075 (≠ V200)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
27% identity, 47% coverage: 91:225/285 of query aligns to 965:1098/1169 of 6ciqA

query
sites
6ciqA

G
|
G

D
|
D

G
|
G

D

W

C

A

Y

Y

-

D

-

I

G

G

E

Y

G

G

-

L

N

D

H

H

F

V

L

L

A

A

A

S

I

-

R

-

N

G

I

A

D

N

M

V

T

N

L

V

L

L

V

V

H

L

N

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

S

T

S

Q

L

T

G

G

M

A

V

V

T

A

K

R

L

F

Q

A

N

A

S

G

G

G

T

K

P

F

S

T

S

K

Q

K

F

K

S

D

A

L

L

G

V

L

I

M

A

A

L

M

A

S

A

Y

G

G

A

Y

G

V

F

Y

V

V

A

A

R

S

-

V

G

A

L

M

S

G

A

A

E

S

S

H

G

S

H

Q

L

L

T

M

E

K

L

A

I

L

V

I

K

E

A

A

M

E

N

K

Y

Y

K

D

G

G

F

P

A

S

L

L

V

I

D

I

I

A

L

Y

Q

A

P

P

C
|
C

V
x
I

S

N

F

-

N

H

K

G

I

I

N

N

T

-

L

M

S

T

W

Y

Y

S

K

Q

E

R

R

E

A

A

Y

K

K

K

L

A

A

V

D

E

T

A

D

G

H

Y

D

W

P

P

active site: N999 (≠ L125)
binding coenzyme a: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199), I1075 (≠ V200)
binding thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
27% identity, 47% coverage: 91:225/285 of query aligns to 965:1098/1165 of 6cipA

query
sites
6cipA

G
|
G

D
|
D

G
|
G

D

W

C

A

Y

Y

-

D

-

I

G

G

E

Y

G

G

-

L

N

D

H

H

F

V

L

L

A

A

A

S

I

-

R

-

N

G

I

A

D

N

M

V

T

N

L

V

L

L

V

V

H

L

N

D

N
x
T

Q

E

I
x
V

Y

Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

S

T

S

Q

L

T

G

G

M

A

V

V

T

A

K

R

L

F

Q

A

N

A

S

G

G

G

T

K

P

F

S

T

S

K

Q

K

F

K

S

D

A

L

L

G

V

L

I

M

A

A

L

M

A

S

A

Y

G

G

A

Y

G

V

F

Y

V

V

A

A

R

S

-

V

G

A

L

M

S

G

A

A

E

S

S

H

G

S

H

Q

L

L

T

M

E

K

L

A

I

L

V

I

K

E

A

A

M

E

N

K

Y

Y

K

D

G

G

F

P

A

S

L

L

V

I

D

I

I

A

L

Y

Q

A

P

P

C
|
C

V

I

S

N

F

-

N

H

K

G

I

I

N

N

T

-

L

M

S

T

W

Y

Y

S

K

Q

E

R

R

E

A

A

Y

K

K

K

L

A

A

V

D

E

T

A

D

G

H

Y

D

W

P

P

active site: N999 (≠ L125)
binding 2-acetyl-thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), S998 (≠ G124), N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
27% identity, 47% coverage: 91:225/285 of query aligns to 965:1098/1169 of 6cinB

query
sites
6cinB

G
|
G

D
|
D

G
|
G

D

W

C

A

Y

Y

-

D

-

I

G

G

E

Y

G

G

-

L

N

D

H

H

F

V

L

L

A

A

A

S

I

-

R

-

N

G

I

A

D

N

M

V

T

N

L

V

L

L

V

V

H

L

N

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

A

S

S

S

P

K

T

A

S

T

S

Q

L

T

G

G

M

A

V

V

T

A

K

R

L

F

Q

A

N

A

S

G

G

G

T

K

P

F

S

T

S

K

Q

K

F

K

S

D

A

L

L

G

V

L

I

M

A

A

L

M

A

S

A

Y

G

G

A

Y

G

V

F

Y

V

V

A

A

R

S

-

V

G

A

L

M

S

G

A

A

E

S

S

H

G

S

H

Q

L

L

T

M

E

K

L

A

I

L

V

I

K

E

A

A

M

E

N

K

Y

Y

K

D

G

G

F

P

A

S

L

L

V

I

D

I

I

A

L

Y

Q

A

P

P

C
|
C

V
x
I

S

N

F

-

N

H

K

G

I

I

N

N

T

-

L

M

S

T

W

Y

Y

S

K

Q

E

R

R

E

A

A

Y

K

K

K

L

A

A

V

D

E

T

A

D

G

H

Y

D

W

P

P

active site: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199), I1075 (≠ V200)
binding thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

Query Sequence

>WP_035237565.1 NCBI__GCF_000745975.1:WP_035237565.1
MITSKEFNCNYENKWCPGCGNFQILAAMKNAFAQQQIPPEKLTLISGIGQAGKTPHFLKC
NMFHGLHGRALPLATGTKIANNDLTVVVNCGDGDCYGEGGNHFLAAIRRNIDMTLLVHNN
QIYGLTKGQASPTSSLGMVTKLQNSGTPSSQFSALVIALAAGAGFVARGLSAESGHLTEL
IVKAMNYKGFALVDILQPCVSFNKINTLSWYKERAYKLADTDHDPRDLAKAFNLAQEWEK
SLPLGVLYEAAGTPFHERVPNLKANALASHNYDSKLLTDVLLQNL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory