SitesBLAST
Comparing WP_035237801.1 NCBI__GCF_000745975.1:WP_035237801.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
34% identity, 100% coverage: 1:259/259 of query aligns to 1:259/259 of 5zaiC
- active site: A65 (≠ I66), F70 (≠ L71), S82 (≠ T76), R86 (= R80), G110 (≠ N111), E113 (≠ G114), P132 (≠ T133), E133 (≠ A134), I138 (≠ L139), P140 (≠ N140), G141 (≠ C141), A226 (= A226), F236 (≠ C236)
- binding coenzyme a: K24 (≠ A25), L25 (≠ M26), A63 (= A64), G64 (= G65), A65 (≠ I66), D66 (= D67), I67 (≠ V68), P132 (≠ T133), R166 (≠ L166), F248 (= F248), K251 (= K251)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
34% identity, 98% coverage: 1:254/259 of query aligns to 1:252/255 of 3q0jC
- active site: A65 (≠ I66), M70 (≠ L71), T80 (= T75), F84 (≠ Y79), G108 (≠ N111), E111 (≠ G114), P130 (≠ T133), E131 (≠ A134), V136 (vs. gap), P138 (≠ N140), G139 (≠ C141), L224 (≠ A226), F234 (≠ C236)
- binding acetoacetyl-coenzyme a: Q23 (≠ G24), A24 (= A25), L25 (≠ M26), A27 (≠ T28), A63 (= A64), G64 (= G65), A65 (≠ I66), D66 (= D67), I67 (≠ V68), K68 (≠ N69), M70 (≠ L71), F84 (≠ Y79), G107 (≠ A110), G108 (≠ N111), E111 (≠ G114), P130 (≠ T133), E131 (≠ A134), P138 (≠ N140), G139 (≠ C141), M140 (≠ V142)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
34% identity, 98% coverage: 1:254/259 of query aligns to 1:252/255 of 3q0gC
- active site: A65 (≠ I66), M70 (≠ L71), T80 (= T75), F84 (≠ Y79), G108 (≠ N111), E111 (≠ G114), P130 (≠ T133), E131 (≠ A134), V136 (vs. gap), P138 (≠ N140), G139 (≠ C141), L224 (≠ A226), F234 (≠ C236)
- binding coenzyme a: L25 (≠ M26), A63 (= A64), I67 (≠ V68), K68 (≠ N69), Y104 (≠ A107), P130 (≠ T133), E131 (≠ A134), L134 (≠ V137)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
35% identity, 97% coverage: 6:257/259 of query aligns to 5:255/257 of 6slbAAA
- active site: Q64 (≠ I66), F69 (≠ L71), L80 (≠ Y79), N84 (≠ I83), A108 (≠ N111), S111 (≠ G114), A130 (≠ T133), F131 (≠ A134), L136 (= L139), P138 (vs. gap), D139 (vs. gap), A224 (= A226), G234 (≠ C236)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R60), A62 (= A64), Q64 (≠ I66), D65 (= D67), L66 (≠ V68), Y76 (vs. gap), A108 (≠ N111), F131 (≠ A134), D139 (vs. gap)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
35% identity, 97% coverage: 3:254/259 of query aligns to 2:247/250 of 3q0gD
- active site: A64 (≠ I66), M69 (≠ L71), T75 (= T75), F79 (≠ Y79), G103 (≠ N111), E106 (≠ G114), P125 (≠ T133), E126 (≠ A134), V131 (vs. gap), P133 (≠ N140), G134 (≠ C141), L219 (≠ A226), F229 (≠ C236)
- binding Butyryl Coenzyme A: F225 (= F232), F241 (= F248)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
37% identity, 97% coverage: 6:257/259 of query aligns to 2:243/245 of 6slaAAA
- active site: Q61 (≠ I66), L68 (≠ Y79), N72 (≠ I83), A96 (≠ N111), S99 (≠ G114), A118 (vs. gap), F119 (vs. gap), L124 (= L132), P126 (≠ A134), N127 (= N136), A212 (= A226), G222 (≠ C236)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ M26), A59 (= A64), Q61 (≠ I66), D62 (= D67), L63 (≠ V68), L68 (≠ Y79), Y71 (≠ W82), A94 (= A109), G95 (≠ A110), A96 (≠ N111), F119 (vs. gap), I122 (≠ M130), L124 (= L132), N127 (= N136), F234 (= F248), K237 (= K251)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
34% identity, 99% coverage: 3:259/259 of query aligns to 2:256/256 of 3h81A
- active site: A64 (≠ I66), M69 (≠ L71), T79 (= T75), F83 (≠ Y79), G107 (≠ N111), E110 (≠ G114), P129 (≠ T133), E130 (≠ A134), V135 (vs. gap), P137 (≠ N140), G138 (≠ C141), L223 (≠ A226), F233 (≠ C236)
- binding calcium ion: F233 (≠ C236), Q238 (≠ A241)
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
34% identity, 97% coverage: 4:254/259 of query aligns to 76:328/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
31% identity, 95% coverage: 12:258/259 of query aligns to 13:253/254 of 2dubA
- active site: A67 (≠ I66), M72 (≠ L71), S82 (≠ R80), G105 (≠ N111), E108 (≠ G114), P127 (≠ T133), E128 (≠ A134), T133 (vs. gap), P135 (≠ N140), G136 (≠ C141), K221 (≠ A226), F231 (≠ C236)
- binding octanoyl-coenzyme a: K25 (≠ G24), A26 (= A25), L27 (≠ M26), A29 (≠ T28), A65 (= A64), A67 (≠ I66), D68 (= D67), I69 (≠ V68), K70 (≠ N69), G105 (≠ N111), E108 (≠ G114), P127 (≠ T133), E128 (≠ A134), G136 (≠ C141), A137 (≠ V142)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
31% identity, 95% coverage: 12:258/259 of query aligns to 14:257/258 of 1mj3A
- active site: A68 (≠ I66), M73 (≠ L71), S83 (≠ E81), L85 (≠ I83), G109 (≠ N111), E112 (≠ G114), P131 (≠ T133), E132 (≠ A134), T137 (vs. gap), P139 (≠ N140), G140 (≠ C141), K225 (≠ A226), F235 (≠ C236)
- binding hexanoyl-coenzyme a: K26 (≠ G24), A27 (= A25), L28 (≠ M26), A30 (≠ T28), A66 (= A64), G67 (= G65), A68 (≠ I66), D69 (= D67), I70 (≠ V68), G109 (≠ N111), P131 (≠ T133), E132 (≠ A134), L135 (≠ V137), G140 (≠ C141)
Q62651 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial; EC 5.3.3.- from Rattus norvegicus (Rat) (see paper)
29% identity, 97% coverage: 3:252/259 of query aligns to 54:317/327 of Q62651
- D176 (≠ G114) mutation D->A,D: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- E196 (≠ A134) mutation E->D,Q: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
- D204 (vs. gap) mutation D->A,N: Strongly decreases dienoyl-CoA and trienoyl-CoA isomerase activity.
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
31% identity, 95% coverage: 12:258/259 of query aligns to 14:259/260 of 1dubA
- active site: A68 (≠ I66), M73 (≠ L71), S83 (≠ E87), L87 (= L91), G111 (≠ N111), E114 (≠ G114), P133 (≠ T133), E134 (≠ A134), T139 (vs. gap), P141 (≠ N140), G142 (≠ C141), K227 (≠ A226), F237 (≠ C236)
- binding acetoacetyl-coenzyme a: K26 (≠ G24), A27 (= A25), L28 (≠ M26), A30 (≠ T28), A66 (= A64), A68 (≠ I66), D69 (= D67), I70 (≠ V68), Y107 (≠ A107), G110 (≠ A110), G111 (≠ N111), E114 (≠ G114), P133 (≠ T133), E134 (≠ A134), L137 (≠ V137), G142 (≠ C141), F233 (= F232), F249 (= F248)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
31% identity, 95% coverage: 12:258/259 of query aligns to 12:257/258 of 1ey3A
- active site: A66 (≠ I66), M71 (≠ L71), S81 (≠ E87), L85 (= L91), G109 (≠ N111), E112 (≠ G114), P131 (≠ T133), E132 (≠ A134), T137 (vs. gap), P139 (≠ N140), G140 (≠ C141), K225 (≠ A226), F235 (≠ C236)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ G24), L26 (≠ M26), A28 (≠ T28), A64 (= A64), G65 (= G65), A66 (≠ I66), D67 (= D67), I68 (≠ V68), L85 (= L91), W88 (vs. gap), G109 (≠ N111), P131 (≠ T133), L135 (≠ V137), G140 (≠ C141)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 93% coverage: 19:259/259 of query aligns to 19:261/261 of 5jbxB
- active site: A67 (≠ I66), R72 (≠ L71), L84 (≠ I83), R88 (≠ E87), G112 (≠ N111), E115 (≠ G114), T134 (= T133), E135 (≠ A134), I140 (≠ L139), P142 (≠ N140), G143 (≠ C141), A228 (= A226), L238 (≠ C236)
- binding coenzyme a: S24 (≠ G24), R25 (≠ A25), R26 (≠ M26), A28 (≠ T28), A65 (= A64), D68 (= D67), L69 (≠ V68), K70 (≠ N69), L110 (≠ A109), G111 (≠ A110), T134 (= T133), E135 (≠ A134), L138 (≠ V137), R168 (≠ L166)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 95% coverage: 12:258/259 of query aligns to 44:289/290 of P14604
- E144 (≠ G114) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ A134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
31% identity, 94% coverage: 14:257/259 of query aligns to 20:266/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
34% identity, 93% coverage: 13:254/259 of query aligns to 18:266/275 of 4i52A
- active site: G77 (≠ I66), R82 (≠ K72), Y87 (≠ W82), R95 (≠ L90), L99 (vs. gap), G123 (≠ N111), V126 (≠ G114), G146 (≠ A134), S151 (≠ L139), D153 (≠ C141), G154 (≠ V142), A240 (≠ Y227), Y248 (≠ L235)
- binding 1-hydroxy-2-naphthoyl-CoA: H29 (≠ G24), K30 (≠ A25), R31 (≠ M26), A33 (≠ T28), S75 (≠ A64), G76 (= G65), G77 (≠ I66), D78 (= D67), Q79 (≠ N69), L96 (= L91), V98 (vs. gap), Y119 (≠ A107), I121 (≠ A109), G123 (≠ N111), T145 (= T133), V149 (= V137), S151 (≠ L139), F152 (≠ N140)
4i4zA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with salicylyl-coa (see paper)
34% identity, 93% coverage: 13:254/259 of query aligns to 18:266/275 of 4i4zA
- active site: G77 (≠ I66), R82 (≠ K72), Y87 (≠ W82), R95 (≠ L90), L99 (vs. gap), G123 (≠ N111), V126 (≠ G114), G146 (≠ A134), S151 (≠ L139), D153 (≠ C141), G154 (≠ V142), A240 (≠ Y227), Y248 (≠ L235)
- binding Salicylyl CoA: H29 (≠ G24), K30 (≠ A25), R31 (≠ M26), S75 (≠ A64), G76 (= G65), G77 (≠ I66), D78 (= D67), Q79 (≠ N69), Y87 (≠ W82), V98 (vs. gap), G123 (≠ N111), T145 (= T133), V149 (= V137), S151 (≠ L139), F260 (= F248), K263 (= K251)
- binding bicarbonate ion: G122 (≠ A110), Q144 (≠ L132), T145 (= T133), G146 (≠ A134), W174 (≠ L161)
Q5HH38 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Staphylococcus aureus (strain COL) (see paper)
34% identity, 97% coverage: 3:254/259 of query aligns to 12:264/273 of Q5HH38
- R34 (≠ M26) binding in other chain
- SGGD-Q 73:77 (≠ AGIDVN 64:69) binding in other chain
- S149 (≠ L139) binding in other chain
4emlA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with bicarbonate (see paper)
34% identity, 93% coverage: 13:254/259 of query aligns to 18:252/261 of 4emlA
- active site: G77 (≠ I66), R81 (≠ E70), L85 (≠ K74), G109 (≠ N111), V112 (≠ G114), G132 (≠ A134), S137 (≠ L139), D139 (≠ C141), G140 (≠ V142), A226 (≠ Y227), Y234 (≠ L235)
- binding bicarbonate ion: G108 (≠ A110), Q130 (≠ L132), G132 (≠ A134), W160 (≠ L161)
- binding chloride ion: D184 (≠ E185), R185 (≠ D186), E187 (≠ D188), E188 (= E189)
Query Sequence
>WP_035237801.1 NCBI__GCF_000745975.1:WP_035237801.1
MAYETIIAQTLDGHVGSVTLNRAGAMNTFSSQMAEELYDALKEFDAASDVRVILIKGAGR
AFCAGIDVNELKGKTTNQYREWIETMEAPLLLISKLKTPVIAQVHGAAAANGMGLVAASD
LAIAADNVKMGLTAINVGLNCVGPVIPVARCVGQKKALELLLYGDLIKADQALELGLINR
VVPREDLDEAALAWARDLAKKSPVAVKIAKSAFYASRDMPYEAQFAYMNEAFARLCDTCD
AKEGVAAFFEKRPPIWQEK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory