SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035238797.1 NCBI__GCF_000745975.1:WP_035238797.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
44% identity, 96% coverage: 3:304/313 of query aligns to 4:307/310 of P9WP55

query
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P9WP55

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K44 (= K42) modified: N6-(pyridoxal phosphate)lysine
N74 (= N72) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GSGGT 176:180) binding pyridoxal 5'-phosphate
S266 (= S264) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
44% identity, 96% coverage: 3:303/313 of query aligns to 4:306/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K42), T71 (= T69), S72 (= S70), N74 (= N72), T75 (= T73), Q144 (= Q142), V177 (≠ L175), G178 (= G176), T179 (≠ S177), G180 (= G178), T182 (= T180), G222 (= G220), I223 (= I221), S266 (= S264), P293 (= P290), D294 (= D291)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 94% coverage: 10:303/313 of query aligns to 7:302/302 of 2efyA

query
sites
2efyA

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active site: K40 (= K42), S70 (= S70), E200 (= E201), S204 (≠ A205), S263 (= S264)
binding 5-oxohexanoic acid: T69 (= T69), G71 (= G71), T73 (= T73), Q141 (= Q142), G175 (= G176), G219 (= G220), M220 (≠ I221), P222 (≠ D223)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S173), G175 (= G176), T176 (≠ S177), G177 (= G178), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290), D290 (= D291)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 94% coverage: 10:303/313 of query aligns to 7:302/302 of 2ecqA

query
sites
2ecqA

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S

S

T

T

A

P

L

L

F

Y

A

A

active site: K40 (= K42), S70 (= S70), E200 (= E201), S204 (≠ A205), S263 (= S264)
binding (3s)-3-hydroxybutanoic acid: K40 (= K42), G71 (= G71), T73 (= T73), Q141 (= Q142), G219 (= G220)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S173), G173 (= G174), G175 (= G176), T176 (≠ S177), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 94% coverage: 10:303/313 of query aligns to 7:302/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

A

K

T

T

P

P

M

V

F

V

R

R

I

L

G

A

L

K

G

V

N

E

D

P

D

D

M

M

-

A

N

E

I

V

Y

W

A

V

K

K

A

L

E

E

Y

G

L

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

V

P

A

A

L

W

F

Y

I

M

I

I

E

K

Q

D

A

A

E

E

K

E

K

R

G

G

R

I

L

L

K

R

-

P

-

G

K

S

G

G

M

Q

S

V

I

I

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

V

L

A

A

M

M

V

I

G

A

L

A

V

S

K

R

G

G

Y

Y

D

R

V

L

R

I

I

L

I

T

M

M

P

P

E

A

N

Q

M

M

S

S

D

E

E

E

R

R

K

K

K

R

M

V

I

L

R

K

A

A

L

F

N

G

A

A

E

E

L

L

I

V

L

L

T

T

P

D

P

P

E

E

K

R

N

R

V

M

A

L

G

A

A

A

V

R

E

E

K

E

L

A

K

L

E

R

I

-

M

L

A

K

E

E

D

E

D

L

N

G

I

A

F

F

V

M

P

P

D

D

Q
|
Q

F

F

E

K

N

N

H

P

D

A

N

N

S

V

M

R

S

A

H

H

Y

Y

L

E

S

T

T

T

G

G

P

P

E

E

I

L

W

Y

K

E

N

A

M

L

N

E

G

G

H

R

V

I

D

D

I

A

F

F

V

V

S
x
Y

G

G

L

S

G
|
G

S
x
T

G

G

T
|
T

L

I

M

T

G

G

T

V

G

G

K

R

Y

Y

L

L

K

K

E

E

K

R

N

I

P

P

E

H

I

V

M

K

I

V

V

I

A

A

V

V

E
|
E

P

P

K

A

N

R

A
x
S

S

N

A

V

L

L

L

S

G

G

H

G

E

K

P

M

G

G

L

Q

H

H

K

G

I

F

E

Q

G
|
G

I

M

G

G

D

P

G

G

F

F

V

I

P

P

S

E

I

N

V

L

D

D

T

L

S

S

L

L

I

L

D

D

N

G

V

V

I

I

E

Q

V

V

D

W

D

E

S

D

A

A

V

F

E

P

M

L

T

A

R

R

W

R

L

L

A

A

S

R

K

E

Q

E

G

G

F

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

V

V

C

W

A

A

L

L

E

Q

M

V

R

A

H

R

L

E

F

L

G

G

K

P

D

G

K

K

N

R

I

V

A

A

T

C

I

I

F

S

P
|
P

D
|
D

G

G

A

G

E

W

R

K

Y

Y

F

L

S

S

T

T

A

P

L

L

F

Y

A

A

active site: K40 (= K42), S70 (= S70), E200 (= E201), S204 (≠ A205), S263 (= S264)
binding 4-methyl valeric acid: K40 (= K42), T69 (= T69), G71 (= G71), T73 (= T73), Q141 (= Q142), G175 (= G176), T176 (≠ S177), G219 (= G220)
binding pyridoxal-5'-phosphate: K40 (= K42), N72 (= N72), Y172 (≠ S173), G175 (= G176), T176 (≠ S177), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290), D290 (= D291)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
46% identity, 94% coverage: 10:304/313 of query aligns to 17:313/329 of 8b9wA

query
sites
8b9wA

I

I

G

G

A

N

T

T

P

P

M

A

F

V

R

Y

I

L

G

R

L

N

G

M

G

N

N

K

D

T

D

A

M

A

N

T

I

I

Y

V

A

L

K

K

A

L

E

E

Y

S

L

E

N

N

P

P

G

M

G

A

S

S

I

V

K
|
K

D

D

R

R

V

L

A

A

L

Y

F

A

I

I

I

Y

E

D

Q

K

A

A

E

E

K

K

K

E

G

G

R

K

L

I

K

K

K

R

G

G

M

K

S

S

-

V

I

I

V

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

V

L

A

A

M

L

V

L

G

G

L

A

V

V

K

R

G

G

Y

Y

D

K

V

V

R

I

I

I

I

T

M

M

P

P

E

D

N

S

M

M

S

S

D

M

E

E

R

R

K

R

K

S

M

L

I

L

R

R

A

I

L

F

N

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

P

A

E

S

K

L

N

G

V

M

A

K

G

G

A

A

V

V

E

A

K

M

L

A

K

N

E

R

I

I

M

V

A

S

E

N

D

N

D

P

N

D

I

A

F

V

V

L

P

A

D

D

Q

Q

F

F

E

C

N

T

H

K

D

Y

N

N

S

A

M

Q

S

I

H

H

Y

E

L

E

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

R

N

Q

M

T

N

K

G

G

H

H

V

V

D

D

I

C

F

F

V

V

S

A

G

G

L

V

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

L

M

T

G

G

T

V

G

A

K

R

Y

F

L

L

K

K

E

S

K

V

N

G

P

C

E

N

I

A

M

N

I

I

V

V

A

A

V

V

E

E

P

P

K

Q

N

E

A

S

S

P

A

V

L

L

L

S

G

G

H

G

E

R

P

P

G

G

L

P

H

H

K

R

I

I

E

Q

G
|
G

I

I

G

G

D

A

G

G

F

F

V

V

P

P

S

E

I

V

V

L

D

D

T

R

S

E

L

L

I

V

D

D

N

E

V

I

I

I

E

Q

V

V

D

S

D

D

S

K

A

A

V

I

E

E

M

T

T

A

R

Q

W

K

L

L

A

S

S

R

K

M

Q

D

G

G

F

I

L

F

V

C

G

G

I

F

S
|
S

S

A

G

G

A

A

N

N

V

V

C

F

A

A

L

L

-

K

-

I

-

A

E

E

M

R

R

P

H

E

L

M

F

A

G

G

K

-

D

-

K

K

N

T

I

I

A

V

T

T

I

V

F

I

P
|
P

D

S

G

F

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

A

L

L

F

Y

A

A

D

S

binding pyridoxal-5'-phosphate: K49 (= K42), N80 (= N72), G184 (= G176), T185 (≠ S177), G186 (= G178), T188 (= T180), G228 (= G220), S272 (= S264), P299 (= P290)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
45% identity, 94% coverage: 10:302/313 of query aligns to 12:306/310 of 5xoqA

query
sites
5xoqA

I

I

G

G

A

R

T

T

P

P

M

L

F

V

R

Q

I

I

G

N

-

R

L

L

G

T

G

A

N

G

D

L

D

S

M

S

N

R

I

V

Y

L

A

A

K

K

A

I

E

E

Y

G

L

R

N

N

P

P

G

A

G

Y

S

S

I

V

K

K

D

C

R

R

V

I

A

G

L

A

F

A

I

M

I

I

E

W

Q

D

A

A

E

E

K

Q

K

S

G

G

R

K

L

L

K

K

K

P

G

G

M

M

S

H

I

V

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

V

L

A

A

M

F

V

V

G

C

L

A

V

A

K

R

G

G

Y

Y

D

K

V

L

R

T

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

I

E

E

R

R

K

R

K

M

M

M

I

L

R

K

A

S

L

F

N

G

A

A

E

D

L

L

I

V

L

L

T

T

P

P

P

G

E

A

K

D

N

G

V
x
M

A

K

G

G

A

A

V

I

E

S

K

K

L

A

K

E

E

E

I

L

M

A

A

A

E

Q

D

-

D

P

N

G

I

W

F

F

V

I

P

P

D

Q

Q
|
Q

F

F

E

K

N

N

H

P

D

A

N

N

S

P

M

A

S

I

H

H

Y

V

L

K

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

N

N

D

M

T

N

E

G

G

H

Q

V

V

D

D

I

V

F

F

V

V

S

A

G

G

L

V

G

G

S

T

G

G

T

T

L

I

M

T

G

G

T

V

G

A

K

R

Y

F

L

L

K

K

-

H

E

E

K

K

N

K

P

H

E

P

I

V

M

H

I

V

V

V

A

A

V

V

E

E

P

P

K

A

N

A

A

S

S

P

A

V

L

L

L

A

G

G

H

G

E

P

P

A

G

G

L
x
R

H
|
H

K

K

I

I

E
x
Q

G
|
G

I

I

G

G

D
x
A

G

G

F

F

V

V

P

P

S

D

I

T

V

F

D

D

T

R

S

S

L

V

I

V

D

D

N

E

V

I

I

L

E

S

V

V

D

T

D

D

S

E

A

A

V

I

E

E

M

T

T

A

R

R

W

K

L

L

A

A

S

M

K

E

Q

E

G

G

F

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

N

A

V

M

C

A

A

G

A

A

L

L

E

K

M

V

-

A

R

R

H

P

L

E

F

F

G

A

K

-

D

G

K

K

N

T

I

I

A

V

T

T

I

V

F

L

P

P

D

D

G

A

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

A

L

L

F

F

binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), M123 (≠ V120), Q144 (= Q142), R218 (≠ L215), H219 (= H216), Q222 (≠ E219), G223 (= G220), A226 (≠ D223)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
47% identity, 92% coverage: 27:313/313 of query aligns to 31:318/322 of P47998

query
sites
P47998

N

R

I

V

Y

A

A

A

K

K

A

L

E

E

Y

M

L

M

N

E

P

P

G

C

G

S

S

S

I

V

K
|
K

D

D

R

R

V

I

A

G

L

F

F

S

I

M

I

I

E

S

Q

D

A

A

E

E

K

K

G

G

R

L

L

I

K

K

K

P

G

G

M

E

S

S

I

V

-

L

V

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

V

L

A

A

M

F

V

T

G

A

L

A

V

A

K

K

G

G

Y

Y

D

K

V

L

R

I

I

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

D

T

E

E

R

R

K

R

K

I

M

I

I

L

R

L

A

A

L

F

N

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

P

P

E

A

K

K

N

G

V

M

A

K

G

G

A

A

V

I

E

A

K

K

L

A

K

E

E

E

I

I

M

L

A

A

E

K

D

T

D

P

N

N

I

G

F

Y

V

M

P

L

D

Q

Q
|
Q

F

F

E

E

N

N

H

P

D

A

N

N

S

P

M

K

S

I

H
|
H

Y

Y

L

E

S

T

T

T

G
|
G

P

P

E

E

I

I

W

W

K

K

N

G

M

T

N

G

G

G

H

K

V

I

D

D

I

G

F

F

V

V

S

S

G

G

L

I

G
|
G

S
x
T

G
|
G

T
|
T

L

I

M

T

G

G

T

A

G

G

K

K

Y

Y

L

L

K

K

E

E

K

Q

N

N

P

A

E

N

I

V

M

K

I

L

V

Y

A

G

V

V

E

E

P

P

K

V

N

E

A

S

S

A

A

I

L

L

L

S

G

G

H

G

E
x
K

P

P

G

G

L

P

H
|
H

K
|
K

I

I

E

Q

G

G

I

I

G

G

D

A

G

G

F

F

V

I

P

P

S

S

I

V

V

L

D

N

T

V

S

D

L

L

I

I

D

D

N

E

V

V

I

V

E

Q

V

V

D

S

D

D

S

E

A

S

V

I

E

D

M

M

T

A

R

R

W

Q

L

L

A

A

S

L

K

K

Q

E

G

G

F

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

C

A

A

A

L

I

E

K

M

L

-

A

R

Q

H

R

L

P

F

E

G

N

K

A

D

G

K

K

N

L

I

F

A

V

T

A

I

I

F

F

P

P

D

S

G

F

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

V

L

L

F

F

A

-

D

D

R

A

H

T

R

R

K

K

L

E

A

A

V

E

A

A

I

M

K46 (= K42) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T69) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S70) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N72) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T73) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q142) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H152) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G157) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GSGGT 176:180) binding pyridoxal 5'-phosphate
T182 (≠ S177) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T180) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ E212) mutation to A: Impaired interaction with SAT1.
H221 (= H216) mutation to A: Impaired interaction with SAT1.
K222 (= K217) mutation to A: Impaired interaction with SAT1.
S269 (= S264) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
47% identity, 92% coverage: 27:313/313 of query aligns to 29:316/320 of 2isqA

query
sites
2isqA

N

R

I

V

Y

A

A

A

K

K

A

L

E

E

Y

M

L

M

N

E

P

P

G

C

G

S

S

S

I

V

K
|
K

D

D

R

R

V

I

A

G

L

F

F

S

I

M

I

I

E

S

Q

D

A

A

E

E

K

K

G

G

R

L

L

I

K

K

K

P

G

G

M

E

S

S

I

V

-

L

V

I

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

V

L

A

A

M

F

V

T

G

A

L

A

V

A

K

K

G

G

Y

Y

D

K

V

L

R

I

I

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

D

T

E

E

R

R

K

R

K

I

M

I

I

L

R

L

A

A

L

F

N

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

P

P

E

A

K

K

N
x
G

V
x
M

A
x
K

G

G

A

A

V

I

E

A

K

K

L

A

K

E

E

E

I

I

M

L

A

A

E

K

D

T

D

P

N

N

I

G

F

Y

V

M

P

L

D

Q

Q

Q

F

F

E

E

N

N

H

P

D

A

N

N

S

P

M

K

S

I

H

H

Y

Y

L

E

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

K

N

G

M

T

N

G

G

G

H

K

V

I

D

D

I

G

F

F

V

V

S

S

G
|
G

L

I

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

T

A

G

G

K

K

Y

Y

L

L

K

K

E

E

K

Q

N

N

P

A

E

N

I

V

M

K

I

L

V

Y

A

G

V

V

E

E

P

P

K

V

N

E

A

S

S

A

A

I

L

L

L

S

G

G

H

G

E

K

P

P

G
|
G

L
x
P

H
|
H

K

K

I

I

E
x
Q

G
|
G

I

I

G

G

D

A

G

G

F

F

V

I

P

P

S

S

I

V

V

L

D

N

T

V

S

D

L

L

I

I

D

D

N

E

V

V

I

V

E

Q

V

V

D

S

D

D

S

E

A

S

V

I

E

D

M

M

T

A

R

R

W

Q

L

L

A

A

S

L

K

K

Q

E

G

G

F

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

C

A

A

A

L

I

E

K

M

L

-

A

R

Q

H

R

L

P

F

E

G

N

K

A

D

G

K

K

N

L

I

F

A

V

T

A

I

I

F

F

P
|
P

D

S

G

F

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

V

L

L

F

F

A

-

D

D

R

A

H

T

R

R

K

K

L

E

A

A

V

E

A

A

I

M

active site: K44 (= K42), S267 (= S264)
binding pyridoxal-5'-phosphate: K44 (= K42), N75 (= N72), G177 (= G174), G179 (= G176), T180 (≠ S177), G181 (= G178), T183 (= T180), G223 (= G220), S267 (= S264), P294 (= P290)
binding : T72 (= T69), S73 (= S70), G74 (= G71), T76 (= T73), G122 (≠ N119), M123 (≠ V120), K124 (≠ A121), G217 (= G214), P218 (≠ L215), H219 (= H216), Q222 (≠ E219), G223 (= G220)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
43% identity, 94% coverage: 3:297/313 of query aligns to 4:300/300 of 3zeiA

query
sites
3zeiA

S

A

N

E

E

D

I

I

L

T

N

Q

Q

L

I

I

G

G

A

R

T

T

P

P

M

L

F

V

R

R

I

L

G

R

L

R

G

V

G

T

N

D

D

G

D

A

M

V

-

A

N

D

I

I

Y

V

A

A

K

K

A

L

E

E

Y

F

L

F

N

N

P

P

G

A

G

N

S

S

I

V

K
|
K

D

D

R

R

V

I

A

G

L

V

F

A

I

M

I

L

E

Q

Q

A

A

A

E

E

K

Q

K

A

G

G

R

L

L

I

K

K

K

P

G

D

M

T

S

I

I

I

V

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

V

L

A

A

M

M

V

V

G

C

L

A

V

A

K

R

G

G

Y

Y

D

R

V

C

R

V

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

L

E

E

R

R

K

R

K

M

M

L

I

L

R

R

A

A

L

Y

N

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

P

G

E

A

K

D

N

G

V

M

A

S

G

G

A

A

V
x
I

E

A

K

K

L

A

K

E

E

E

I

L

M

A

A

K

E

T

D

D

D

Q

N

R

I

Y

F

F

V

V

P

P

D

Q

Q
|
Q

F
|
F

E

E

N

N

H

P

D

A

N

N

S

P

M

A

S

I

H

H

Y

R

L

V

S

T

T

T

G

A

P

E

E

E

I

V

W

W

K

R

N

D

M

T

N

D

G

G

H

K

V

V

D

D

I

I

F

V

V

V

S

A

G

G

L
x
V

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

T

V

G

A

K

Q

Y

V

L

I

K

K

E

E

K

R

N

K

P

P

E

S

I

A

M

R

I

F

V

V

A

A

V

V

E

E

P

P

K

A

N

A

A

S

S

P

A

V

L

L

L

S

G

G

H

G

E

Q

P
x
K

G

G

L

P

H

H

K

P

I

I

E

Q

G
|
G

I

I

G

G

D
x
A

G

G

F
|
F

V

V

P

P

S

P

I

V

V

L

D

D

T

Q

S

D

L

L

I

V

D

D

N

E

V

I

I

I

E

T

V

V

D

G

D

N

D

E

S

D

A

A

V

L

E

N

M

V

T

A

R

R

W

R

L

L

A

A

S

R

K

E

Q

E

G

G

F

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

T

C

V

A

A

L

L

E

Q

M

V

-

A

R

R

H

R

L

P

F

E

G

N

K

A

D

G

K

K

N

L

I

I

A

V

T

V

I

V

F

L

P
|
P

D
|
D

G

F

A

G

E

E

R

R

Y

Y

F

L

active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T69), S72 (= S70), I126 (≠ V124), Q144 (= Q142), F145 (= F143), K215 (≠ P213), G222 (= G220), A225 (≠ D223), F227 (= F225)
binding pyridoxal-5'-phosphate: K44 (= K42), N74 (= N72), V177 (≠ L175), G178 (= G176), T179 (≠ S177), G180 (= G178), T182 (= T180), G222 (= G220), S266 (= S264), P293 (= P290), D294 (= D291)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
43% identity, 94% coverage: 3:297/313 of query aligns to 4:300/300 of 2q3cA

query
sites
2q3cA

S

A

N

E

E

D

I

I

L

T

N

Q

Q

L

I

I

G

G

A

R

T

T

P

P

M

L

F

V

R

R

I

L

G

R

L

R

G

V

G

T

N

D

D

G

D

A

M

V

-

A

N

D

I

I

Y

V

A

A

K

K

A

L

E

E

Y

F

L

F

N

N

P

P

G

A

G

N

S

S

I

V

K
|
K

D

D

R

R

V

I

A

G

L

V

F

A

I

M

I

L

E

Q

Q

A

A

A

E

E

K

Q

K

A

G

G

R

L

L

I

K

K

K

P

G

D

M

T

S

I

I

I

V

L

E

E

A

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

V

L

A

A

M

M

V

V

G

C

L

A

V

A

K

R

G

G

Y

Y

D

R

V

C

R

V

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

L

E

E

R

R

K

R

K

M

M

L

I

L

R

R

A

A

L

Y

N

G

A

A

E

E

L

L

I

I

L

L

T

T

P

P

P

G

E

A

K

D

N

G

V
x
M

A

S

G

G

A

A

V

I

E

A

K

K

L

A

K

E

E

E

I

L

M

A

A

K

E

T

D

D

D

Q

N

R

I

Y

F

F

V

V

P

P

D

Q

Q
|
Q

F

F

E

E

N

N

H

P

D

A

N

N

S

P

M

A

S

I

H

H

Y

R

L

V

S

T

T

T

G

A

P

E

E

E

I

V

W

W

K

R

N

D

M

T

N

D

G

G

H

K

V

V

D

D

I

I

F

V

V

V

S

A

G

G

L

V

G

G

S

T

G

G

T

T

L

I

M

T

G

G

T

V

G

A

K

Q

Y

V

L

I

K

K

E

E

K

R

N

K

P

P

E

S

I

A

M

R

I

F

V

V

A

A

V

V

E

E

P

P

K

A

N

A

A

S

S

P

A

V

L

L

L

S

G

G

H

G

E

Q

P
x
K

G

G

L

P

H

H

K

P

I

I

E

Q

G
|
G

I

I

G

G

D
x
A

G

G

F

F

V

V

P

P

S

P

I

V

V

L

D

D

T

Q

S

D

L

L

I

V

D

D

N

E

V

I

I

I

E

T

V

V

D

G

D

N

D

E

S

D

A

A

V

L

E

N

M

V

T

A

R

R

W

R

L

L

A

A

S

R

K

E

Q

E

G

G

F

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

T

C

V

A

A

L

L

E

Q

M

V

-

A

R

R

H

R

L

P

F

E

G

N

K

A

D

G

K

K

N

L

I

I

A

V

T

V

I

V

F

L

P
|
P

D

D

G

F

A

G

E

E

R

R

Y

Y

F

L

active site: K44 (= K42), S266 (= S264), P293 (= P290)
binding : T71 (= T69), S72 (= S70), G73 (= G71), T75 (= T73), M122 (≠ V120), Q144 (= Q142), K215 (≠ P213), G222 (= G220), A225 (≠ D223)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
46% identity, 94% coverage: 10:304/313 of query aligns to 12:314/323 of P0A1E3

query
sites
P0A1E3

I

I

G

G

A

H

T

T

P

P

M

L

F

V

R

R

I

L

G

N

L

R

G

I

G

G

N

N

D

G

D

-

M

-

N

R

I

I

Y

L

A

A

K

K

A

V

E

E

Y

S

L

R

N

N

P

P

G

S

G

F

S

S

I

V

K

K

D

C

R

R

V

I

A

G

L

A

F

N

I

M

I

I

E

W

Q

D

A

A

E

E

K

K

K

R

G

G

R

V

L

L

K

K

K

P

G

G

M

V

S

E

I

L

V

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

V

L

A

A

M

Y

V

V

G

A

L

A

V

A

K

R

G

G

Y

Y

D

K

V

L

R

T

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

I

E

E

R

R

K

R

K

K

M

L

I

L

R

K

A

A

L

L

N

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

P

G

E

A

K

K

N

G

V

M

A

K

G

G

A

A

V

I

E

Q

K

K

L

A

K

E

E

E

I

I

M

V

A

A

E

S

D

D

D

P

N

Q

I

K

F

Y

-

L

V

L

P

L

D

Q

Q

Q

F

F

E

S

N

N

H

P

D

A

N

N

S

P

M

E

S

I

H

H

Y

E

L

K

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

E

N

D

M

T

N

D

G

G

H

Q

V

V

D

D

I

V

F

F

V

I

S

S

G

G

L

V

G

G

S

T

G

G

T

T

L

L

M

T

G

G

T

V

G

T

K

R

Y

Y

L

I

K

K

-

G

-

T

E

K

K

G

N

K

P

T

E

D

I

L

M

I

I

T

V

V

A

A

V

V

E

E

P

P

K

T

N

D

-

S

-

P

-

V

-

I

A

A

S

Q

A

A

L

L

L

A

G

G

H

E

E

E

-

I

-

K

P

P

G

G

L

P

H

H

K

K

I

I

E

Q

G

G

I

I

G

G

D

A

G

G

F

F

V

I

P

P

S

G

I

N

V

L

D

D

T

L

S

K

L

L

I

I

D

D

N

K

V

V

I

V

E

G

V

I

D

T

D

N

D

E

S

E

A

A

V

I

E

S

M

T

T

A

R

R

W

R

L

L

A

M

S

E

K

E

Q

E

G

G

F

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

C

A

A

A

L

L

E

K

M

L

R

Q

H

E

L

D

F

E

G

S

-

F

K

T

D

N

K

K

N

N

I

I

A

V

T

V

I

I

F

L

P

P

D

S

G

S

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

A

L

L

F

F

A

A

D

D

N72 (= N72) binding pyridoxal 5'-phosphate
S273 (= S264) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
46% identity, 94% coverage: 10:304/313 of query aligns to 13:315/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

G

G

A

H

T

T

P

P

M

L

F

V

R

R

I

L

G

N

L

R

G

I

G

G

N

N

D

G

D

-

M

-

N

R

I

I

Y

L

A

A

K

K

A

V

E

E

Y

S

L

R

N

N

P

P

G

S

G

F

S

S

I

V

K
|
K

D

C

R

R

V

I

A

G

L

A

F

N

I

M

I

I

E

W

Q

D

A

A

E

E

K

K

K

R

G

G

R

V

L

L

K

K

K

P

G

G

M

V

S

E

I

L

V

V

E

E

A

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

V

L

A

A

M

Y

V

V

G

A

L

A

V

A

K

R

G

G

Y

Y

D

K

V

L

R

T

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

I

E

E

R

R

K

R

K

K

M

L

I

L

R

K

A

A

L

L

N

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E

P

G

E

A

K

K

N

G

V

M

A

K

G

G

A

A

V

I

E

Q

K

K

L

A

K

E

E

E

I

I

M

V

A

A

E

S

D

D

D

P

N

Q

I

K

F

Y

-

L

V

L

P

L

D

Q

Q
|
Q

F
|
F

E

S

N

N

H

P

D

A

N

N

S

P

M

E

S

I

H

H

Y

E

L

K

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

E

N

D

M

T

N

D

G

G

H

Q

V

V

D

D

I

V

F

F

V

I

S

S

G

G

L
x
V

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

L

M

T

G

G

T

V

G

T

K

R

Y

Y

L

I

K

K

-

G

-

T

E

K

K

G

N

K

P

T

E

D

I

L

M

I

I

T

V

V

A

A

V

V

E

E

P

P

K

T

N

D

-

S

-

P

-

V

-

I

A

A

S

Q

A

A

L

L

L

A

G

G

H

E

E

E

-

I

-

K

P

P

G

G

L

P

H

H

K

K

I

I

E
x
Q

G
|
G

I
|
I

G

G

D
x
A

G

G

F

F

V

I

P

P

S

G

I

N

V

L

D

D

T

L

S

K

L

L

I

I

D

D

N

K

V

V

I

V

E

G

V

I

D

T

D

N

D

E

S

E

A

A

V

I

E

S

M

T

T

A

R

R

W

R

L

L

A

M

S

E

K

E

Q

E

G

G

F

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

C

A

A

A

L

L

E

K

M

L

R

Q

H

E

L

D

F

E

G

S

-

F

K

T

D

N

K

K

N

N

I

I

A

V

T

V

I

I

F

L

P
|
P

D

S

G

S

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

A

L

L

F

F

A

A

D

D

binding pyridoxal-5'-phosphate: K43 (= K42), N73 (= N72), V177 (≠ L175), G178 (= G176), T179 (≠ S177), G180 (= G178), T182 (= T180), G230 (= G220), S274 (= S264), P301 (= P290)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K42), T70 (= T69), G72 (= G71), N73 (= N72), T74 (= T73), Q144 (= Q142), F145 (= F143), Q229 (≠ E219), G230 (= G220), I231 (= I221), A233 (≠ D223)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
47% identity, 92% coverage: 27:313/313 of query aligns to 29:316/320 of 1z7yA

query
sites
1z7yA

N

R

I

V

Y

A

A

A

K

K

A

L

E

E

Y

M

L

M

N

E

P

P

G

C

G

S

S

S

I

V

K
x
A

D

D

R

R

V

I

A

G

L

F

F

S

I

M

I

I

E

S

Q

D

A

A

E

E

K

K

G

G

R

L

L

I

K

K

K

P

G

G

M

E

S

S

I

V

-

L

V

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

V

L

A

A

M

F

V

T

G

A

L

A

V

A

K

K

G

G

Y

Y

D

K

V

L

R

I

I

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

D

T

E

E

R

R

K

R

K

I

M

I

I

L

R

L

A

A

L

F

N

G

A

V

E

E

L

L

I

V

L

L

T

T

P

D

P

P

E

A

K

K

N

G

V

M

A

K

G

G

A

A

V

I

E

A

K

K

L

A

K

E

E

E

I

I

M

L

A

A

E

K

D

T

D

P

N

N

I

G

F

Y

V

M

P

L

D

Q

Q
|
Q

F

F

E

E

N

N

H

P

D

A

N

N

S

P

M

K

S

I

H

H

Y

Y

L

E

S

T

T

T

G

G

P

P

E

E

I

I

W

W

K

K

N

G

M

T

N

G

G

G

H

K

V

I

D

D

I

G

F

F

V

V

S

S

G

G

L
x
I

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

T

A

G

G

K

K

Y

Y

L

L

K

K

E

E

K

Q

N

N

P

A

E

N

I

V

M

K

I

L

V

Y

A

G

V

V

E

E

P

P

K

V

N

E

A

S

S

A

A

I

L

L

L

S

G

G

H

G

E

K

P

P

G

G

L

P

H

H

K

K

I

I

E

Q

G
|
G

I

I

G

G

D

A

G

G

F

F

V

I

P

P

S

S

I

V

V

L

D

N

T

V

S

D

L

L

I

I

D

D

N

E

V

V

I

V

E

Q

V

V

D

S

D

D

S

E

A

S

V

I

E

D

M

M

T

A

R

R

W

Q

L

L

A

A

S

L

K

K

Q

E

G

G

F

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

C

A

A

A

L

I

E

K

M

L

-

A

R

Q

H

R

L

P

F

E

G

N

K

A

D

G

K

K

N

L

I

F

A

V

T

A

I

I

F

F

P
|
P

D
x
S

G

F

A

G

E

E

R

R

Y

Y

F

L

S

S

T

T

A

V

L

L

F

F

A

-

D

D

R

A

H

T

R

R

K

K

L

E

A

A

V

E

A

A

I

M

active site: A44 (≠ K42), S267 (= S264)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G71), N75 (= N72), T76 (= T73), Q145 (= Q142), I178 (≠ L175), G179 (= G176), T180 (≠ S177), G181 (= G178), T183 (= T180), G223 (= G220), S267 (= S264), P294 (= P290), S295 (≠ D291)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 94% coverage: 10:304/313 of query aligns to 12:314/323 of P0ABK5

query
sites
P0ABK5

I

I

G

G

A

H

T

T

P

P

M

L

F

V

R

R

I

L

G

N

L

R

G

I

G

G

N

N

D

G

D

-

M

-

N

R

I

I

Y

L

A

A

K

K

A

V

E

E

Y

S

L

R

N

N

P

P

G

S

G

F

S

S

I

V

K
|
K

D

C

R

R

V

I

A

G

L

A

F

N

I

M

I

I

E

W

Q

D

A

A

E

E

K

K

K

R

G

G

R

V

L

L

K

K

K

P

G

G

M

V

S

E

I

L

V

V

E

E

A

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

V

L

A

A

M

Y

V

V

G

A

L

A

V

A

K

R

G

G

Y

Y

D

K

V

L

R

T

I

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

D

I

E

E

R

R

K

R

K

K

M

L

I

L

R

K

A

A

L

L

N

G

A

A

E

N

L

L

I

V

L

L

T

T

P

E