SitesBLAST
Comparing WP_035239838.1 NCBI__GCF_000745975.1:WP_035239838.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P25526 Succinate-semialdehyde dehydrogenase [NADP(+)] GabD; SSDH; Glutarate-semialdehyde dehydrogenase; EC 1.2.1.79; EC 1.2.1.- from Escherichia coli (strain K12) (see paper)
65% identity, 99% coverage: 2:481/484 of query aligns to 1:480/482 of P25526
3jz4A Crystal structure of e. Coli NADP dependent enzyme (see paper)
65% identity, 99% coverage: 3:481/484 of query aligns to 1:479/481 of 3jz4A
- active site: N156 (= N158), K179 (= K181), E254 (= E256), C288 (= C290), E385 (= E387), E462 (= E464)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: P154 (= P156), W155 (= W157), K179 (= K181), A181 (= A183), S182 (≠ T184), A212 (≠ S214), G216 (= G218), G232 (= G234), S233 (= S235), I236 (≠ V238), C288 (= C290), K338 (≠ T340), E385 (= E387), F387 (= F389)
8of1A Structure of aldh5f1 from moss physcomitrium patens in complex with NAD+ in the contracted conformation
56% identity, 100% coverage: 1:484/484 of query aligns to 17:500/505 of 8of1A
- binding nicotinamide-adenine-dinucleotide: I170 (= I154), A171 (≠ T155), P172 (= P156), W173 (= W157), K197 (= K181), A230 (≠ S214), F248 (= F232), G250 (= G234), S251 (= S235), V254 (= V238), M257 (≠ K241), L273 (= L257), C306 (= C290), K356 (≠ T340), E403 (= E387), F405 (= F389)
8c54A Cryo-em structure of nadh bound sla dehydrogenase rlgabd from rhizobium leguminosarum bv. Trifolii srd1565 (see paper)
59% identity, 99% coverage: 4:484/484 of query aligns to 2:482/482 of 8c54A
- binding 1,4-dihydronicotinamide adenine dinucleotide: I152 (= I154), T153 (= T155), P154 (= P156), K179 (= K181), A212 (≠ S214), K213 (≠ S215), F230 (= F232), T231 (= T233), G232 (= G234), S233 (= S235), V236 (= V238), W239 (≠ K241), G256 (= G258)
P51649 Succinate-semialdehyde dehydrogenase, mitochondrial; Aldehyde dehydrogenase family 5 member A1; NAD(+)-dependent succinic semialdehyde dehydrogenase; EC 1.2.1.24 from Homo sapiens (Human) (see 5 papers)
55% identity, 99% coverage: 6:484/484 of query aligns to 55:535/535 of P51649
- C93 (= C46) to F: in SSADHD; 3% of activity; dbSNP:rs765561257
- G176 (= G129) to R: in SSADHD; <1% of activity; dbSNP:rs72552281
- H180 (≠ P133) to Y: 83% of activity; dbSNP:rs2760118
- P182 (≠ T135) to L: 48% of activity; dbSNP:rs3765310
- R213 (= R166) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- C223 (= C176) to Y: in SSADHD; 5% of activity; dbSNP:rs72552282
- KPAE 228:231 (≠ KPAT 181:184) binding NAD(+)
- T233 (= T186) to M: in SSADHD; 4% of activity; dbSNP:rs1326526453
- A237 (= A190) to S: 65% of activity; dbSNP:rs62621664
- N255 (= N208) to S: in SSADHD; 17% of activity; dbSNP:rs145087265
- G268 (= G218) to E: in SSADHD; <1% of activity; dbSNP:rs375628463
- GSTTTG 284:289 (≠ GSTQVG 234:239) binding NAD(+)
- R334 (= R284) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- N335 (= N285) to K: in SSADHD; 1% of activity; dbSNP:rs72552283
- C340 (= C290) modified: Disulfide link with 342, In inhibited form
- C342 (= C292) modified: Disulfide link with 340, In inhibited form; mutation to A: Loss of regulation by redox state.
- N372 (= N321) natural variant: N -> S
- P382 (= P331) to L: in SSADHD; 2% of activity
- V406 (= V355) to I: in dbSNP:rs143741652
- G409 (= G358) to D: in SSADHD; <1% of activity; dbSNP:rs118203984
- S498 (= S447) binding substrate; mutation to A: Reduces catalytic activity to less than 15% of wild-type.
- G533 (≠ A482) to R: in SSADHD; <1% of activity; dbSNP:rs72552284
Sites not aligning to the query:
- 36 G → R: no effect on succinate-semialdehyde dehydrogenase activity; dbSNP:rs4646832
2w8rA The crystal structure of human ssadh in complex with NAD+ (see paper)
55% identity, 99% coverage: 6:484/484 of query aligns to 5:485/485 of 2w8rA
2w8qA The crystal structure of human ssadh in complex with ssa. (see paper)
55% identity, 99% coverage: 6:484/484 of query aligns to 5:485/485 of 2w8qA
5x5uA Crystal structure of alpha-ketoglutarate-semialdehyde dehydrogenase (kgsadh) complexed with NAD (see paper)
44% identity, 97% coverage: 13:481/484 of query aligns to 6:474/476 of 5x5uA
- active site: N151 (= N158), K174 (= K181), E249 (= E256), C283 (= C290), E380 (= E387), E457 (= E464)
- binding glycerol: D15 (≠ A22), A16 (= A23), A17 (≠ D24), G19 (= G26)
- binding nicotinamide-adenine-dinucleotide: P149 (= P156), P207 (≠ S214), A208 (≠ S215), S211 (≠ G218), G227 (= G234), S228 (= S235), V231 (= V238), R329 (≠ A336), R330 (≠ A337), E380 (= E387), F382 (= F389)
5x5tA Crystal structure of alpha-ketoglutarate semialdehyde dehydrogenase (kgsadh) from azospirillum brasilense (see paper)
44% identity, 97% coverage: 13:481/484 of query aligns to 6:474/476 of 5x5tA
6j76A Structure of 3,6-anhydro-l-galactose dehydrogenase in complex with nap (see paper)
38% identity, 96% coverage: 15:477/484 of query aligns to 5:471/477 of 6j76A