SitesBLAST
Comparing WP_035240196.1 NCBI__GCF_000745975.1:WP_035240196.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6m47A X-ray structure of a drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in tramadol bound form (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 6m47A
6m3zA X-ray structure of a drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in milnacipran bound form (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/536 of 6m3zA
- binding cholesterol: L212 (= L188), I286 (≠ C262)
- binding (1R,2S)-2-(aminomethyl)-N,N-diethyl-1-phenyl-cyclopropane-1-carboxamide: F19 (≠ S20), V96 (≠ I97), Y99 (= Y100), Y100 (= Y101), F254 (= F230), F260 (= F236), S356 (= S332), G360 (≠ V336)
- binding alpha-D-glucopyranose: Y277 (≠ A253), F448 (= F421)
6m0zA X-ray structure of drosophila dopamine transporter with net-like mutations (d121g/s426m/f471l) in l-norepinephrine bound form (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/536 of 6m0zA
4xpfA X-ray structure of drosophila dopamine transporter with subsiteb mutations (d121g/s426m) bound to rti-55 (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:503/532 of 4xpfA
- binding methyl (1R,2S,3S,5S)-3-(4-iodophenyl)-8-methyl-8-azabicyclo[3.2.1]octane-2-carboxylate: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), G97 (≠ S98), Y100 (= Y101), F258 (= F236)
- binding chloride ion: Y45 (= Y46), S253 (= S231), S289 (= S267)
- binding cholesterol: Y206 (≠ F184), L210 (= L188), I284 (≠ C262)
- binding decyl-beta-d-maltopyranoside: R109 (≠ S110), F112 (≠ V113), Y152 (≠ T131), D171 (≠ P148)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S253 (= S231), N285 (= N263), L350 (≠ A328), G351 (= G329), D353 (≠ S331), S354 (= S332)
4xnxA X-ray structure of drosophila dopamine transporter in complex with reboxetine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/536 of 4xnxA
- binding (2R)-2-[(R)-(2-ethoxyphenoxy)(phenyl)methyl]morpholine: F19 (≠ S20), A20 (= A21), D22 (≠ G23), A93 (= A94), V96 (≠ I97), D97 (≠ S98), Y100 (= Y101), S255 (= S231), G360 (≠ V336)
- binding cholesterol: Y208 (≠ F184), L212 (= L188), I286 (≠ C262), Y453 (≠ L426), W454 (= W427)
- binding alpha-D-glucopyranose: R109 (≠ S110), Y154 (≠ T131)
4xp9C X-ray structure of drosophila dopamine transporter bound to psychostimulant d-amphetamine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/537 of 4xp9C
- binding (2S)-1-phenylpropan-2-amine: F19 (≠ S20), D22 (≠ G23), S255 (= S231), F260 (= F236)
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding sodium ion: G18 (= G19), A20 (= A21), V21 (≠ I22), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), G353 (= G329), D355 (≠ S331), S356 (= S332)
- binding cholesterol hemisuccinate: G451 (= G424), W454 (= W427)
4xp1A X-ray structure of drosophila dopamine transporter bound to neurotransmitter dopamine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/535 of 4xp1A
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding cholesterol: L13 (≠ I14), Y208 (≠ F184), L212 (= L188), I286 (≠ C262)
- binding alpha-D-glucopyranose: R109 (≠ S110), Y154 (≠ T131)
- binding l-dopamine: V96 (≠ I97), D97 (≠ S98), Y100 (= Y101), F260 (= F236), G360 (≠ V336)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), G353 (= G329), D355 (≠ S331), S356 (= S332)
- binding cholesterol hemisuccinate: F285 (= F261), F292 (≠ I268), G451 (= G424)
4xpaA X-ray structure of drosophila dopamine transporter bound to 3, 4dichlorophenethylamine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 4xpaA
- binding 2-(3,4-dichlorophenyl)ethanamine: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), F254 (= F230), F260 (= F236), S356 (= S332)
- binding beta-D-glucopyranose: R109 (≠ S110), A113 (≠ L114)
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding cholesterol: A50 (≠ L51), Y208 (≠ F184), I286 (≠ C262), F292 (≠ I268), Y453 (≠ L426), W454 (= W427)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), G353 (= G329), D355 (≠ S331), S356 (= S332)
4xp6A X-ray structure of drosophila dopamine transporter bound to psychostimulant methamphetamine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 4xp6A
- binding (2S)-N-methyl-1-phenylpropan-2-amine: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), F260 (= F236), S356 (= S332)
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding cholesterol: L13 (≠ I14), L205 (= L182), Y208 (≠ F184), L212 (= L188), I286 (≠ C262)
- binding alpha-D-glucopyranose: R109 (≠ S110), A113 (≠ L114)
- binding sodium ion: G18 (= G19), A20 (= A21), V21 (≠ I22), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), G353 (= G329), D355 (≠ S331), S356 (= S332)
- binding cholesterol hemisuccinate: I54 (= I55), Y453 (≠ L426), W454 (= W427)
4xp5A X-ray structure of drosophila dopamine transporter bound to cocaine analogue-rti55 (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 4xp5A
- binding methyl (1R,2S,3S,5S)-3-(4-iodophenyl)-8-methyl-8-azabicyclo[3.2.1]octane-2-carboxylate: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), D97 (≠ S98), Y100 (= Y101), F254 (= F230), S255 (= S231), F260 (= F236), G360 (≠ V336)
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding cholesterol: L13 (≠ I14), L14 (= L15), L49 (≠ M50), A50 (≠ L51), Y208 (≠ F184), I286 (≠ C262), Y453 (≠ L426), W454 (= W427)
- binding alpha-D-glucopyranose: R109 (≠ S110), F112 (≠ V113), A113 (≠ L114), Y154 (≠ T131)
- binding sodium ion: G18 (= G19), A20 (= A21), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), D355 (≠ S331), S356 (= S332)
4xp4A X-ray structure of drosophila dopamine transporter in complex with cocaine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 4xp4A
- binding chloride ion: S255 (= S231), S291 (= S267)
- binding cocaine: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), D97 (≠ S98), Y100 (= Y101), F254 (= F230), S255 (= S231), F260 (= F236), S356 (= S332), G360 (≠ V336)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S255 (= S231), N287 (= N263), L352 (≠ A328), G353 (= G329), D355 (≠ S331), S356 (= S332)
- binding cholesterol hemisuccinate: L49 (≠ M50), I54 (= I55), Y453 (≠ L426), W454 (= W427)
6m38A X-ray structure of a drosophila dopamine transporter with subsiteb mutations (d121g/s426m) in s-duloxetine bound form (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:505/534 of 6m38A
- binding (3S)-N-methyl-3-(naphthalen-1-yloxy)-3-(thiophen-2-yl)propan-1-amine: F19 (≠ S20), A20 (= A21), D22 (≠ G23), A93 (= A94), V96 (≠ I97), Y100 (= Y101), F254 (= F230), S255 (= S231), F260 (= F236), G360 (≠ V336)
- binding cholesterol: Y208 (≠ F184), L212 (= L188), I286 (≠ C262)
- binding decyl-beta-d-maltopyranoside: R109 (≠ S110), F112 (≠ V113), A113 (≠ L114), D173 (≠ P148), L176 (≠ G151)
9euoA Sodium-dependent dopamine transporter (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:503/532 of 9euoA
- binding N-[3-(6-chloranyl-1,3,4,9-tetrahydropyrido[3,4-b]indol-2-yl)propyl]butanamide: R28 (= R29), A93 (= A94), V96 (≠ I97), Y99 (= Y100), F252 (= F230), F258 (= F236)
- binding chloride ion: Y45 (= Y46), S253 (= S231), S289 (= S267)
- binding cholesterol: L13 (≠ I14), I17 (= I18), L210 (= L188), I284 (≠ C262)
- binding sodium ion: A20 (= A21), N25 (= N26), S253 (= S231), N285 (= N263)
- binding cholesterol hemisuccinate: L49 (≠ M50), A50 (≠ L51), I54 (= I55), F283 (= F261), S286 (≠ C264), A287 (≠ G265), Y451 (≠ L426), W452 (= W427)
4xnuA X-ray structure of drosophila dopamine transporter in complex with nisoxetine (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:503/532 of 4xnuA
4m48A X-ray structure of dopamine transporter elucidates antidepressant mechanism (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:503/532 of 4m48A
- binding Nortriptyline: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), Y100 (= Y101), F258 (= F236), S354 (= S332), G358 (≠ V336)
- binding cholesterol: Y206 (≠ F184), L210 (= L188), I284 (≠ C262), I291 (≠ L269)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), V21 (≠ I22), D22 (≠ G23), D22 (≠ G23), N25 (= N26), S253 (= S231), N285 (= N263), L350 (≠ A328), D353 (≠ S331), S354 (= S332)
7wlwA X-ray structure of thermostabilized drosophila dopamine transporter with gaba transporter1-like substitutions in the binding site, in complex with skf89976a (see paper)
30% identity, 97% coverage: 3:477/490 of query aligns to 2:505/536 of 7wlwA
- binding (3S)-1-(4,4-diphenylbut-3-enyl)piperidine-3-carboxylic acid: Y19 (≠ S20), G22 (= G23), L23 (= L24), A24 (≠ G25), L96 (≠ I97), Y99 (= Y100), Y100 (= Y101), T250 (≠ G226), F253 (= F229), F254 (= F230), F254 (= F230), G257 (≠ S233), P258 (≠ I234), L260 (≠ F236), P321 (≠ V297), D410 (= D383), A414 (≠ N387)
- binding cholesterol: I17 (= I18), Y208 (≠ F184), L212 (= L188)
- binding decyl-beta-d-maltopyranoside: R109 (≠ S110), F112 (≠ V113), Y154 (≠ T131), L176 (≠ G151)
- binding cholesterol hemisuccinate: L49 (≠ M50), G451 (= G424), Y453 (≠ L426)
7wgtA X-ray structure of thermostabilized drosophila dopamine transporter with gaba transporter1-like substitutions in the binding site, in complex with no711. (see paper)
30% identity, 97% coverage: 3:477/490 of query aligns to 2:505/536 of 7wgtA
- binding 1-(2-{[(diphenylmethylidene)amino]oxy}ethyl)-1,2,5,6-tetrahydropyridine-3-carboxylic acid: Y19 (≠ S20), G22 (= G23), A24 (≠ G25), L96 (≠ I97), F253 (= F229), F254 (= F230), G257 (≠ S233), P258 (≠ I234), A414 (≠ N387), I418 (≠ V391)
- binding cholesterol: Y208 (≠ F184), L212 (= L188)
- binding cholesterol hemisuccinate: Y277 (≠ A253), S288 (≠ C264), A289 (≠ G265), G451 (= G424), W454 (= W427)
4xpgA X-ray structure of drosophila dopamine transporter with subsiteb mutations (d121g/s426m) bound to beta-cft or win35428 (see paper)
30% identity, 97% coverage: 2:477/490 of query aligns to 1:503/533 of 4xpgA
- binding methyl (1R,2S,3S,5S)-3-(4-fluorophenyl)-8-methyl-8-azabicyclo[3.2.1]octane-2-carboxylate: F19 (≠ S20), D22 (≠ G23), V96 (≠ I97), F252 (= F230), F258 (= F236), G358 (≠ V336)
- binding chloride ion: Y45 (= Y46), S253 (= S231), S289 (= S267)
- binding cholesterol: Y206 (≠ F184), L210 (= L188), I284 (≠ C262)
- binding sodium ion: G18 (= G19), F19 (≠ S20), A20 (= A21), V21 (≠ I22), D22 (≠ G23), N25 (= N26), S253 (= S231), N285 (= N263), L350 (≠ A328), G351 (= G329), D353 (≠ S331), S354 (= S332)
8wtwA Cryo-em structure of noradrenaline transporter in complex with a x- mrla analogue (see paper)
30% identity, 98% coverage: 4:481/490 of query aligns to 2:502/551 of 8wtwA
- binding O-methyl-L-tyrosine: R27 (= R29), F251 (= F230), F257 (= F236)
- binding chloride ion: S252 (= S231), S288 (= S267)
- binding sodium ion: G17 (= G19), F18 (≠ S20), A19 (= A21), V20 (≠ I22), V20 (≠ I22), D21 (≠ G23), N24 (= N26), S252 (= S231), N284 (= N263), L349 (≠ A328), D352 (≠ S331), S353 (= S332)
- binding : W26 (= W28), R27 (= R29), D244 (≠ A223), D407 (= D383), K475 (≠ I451), P476 (≠ K452), T478 (≠ P454)
8zp1A Sodium-dependent noradrenaline transporter (see paper)
30% identity, 98% coverage: 2:481/490 of query aligns to 1:506/555 of 8zp1A
- binding (2~{S})-2-[(~{S})-(2-ethoxyphenoxy)-phenyl-methyl]morpholine: F19 (≠ S20), A20 (= A21), D22 (≠ G23), A92 (= A94), V95 (≠ I97), G96 (≠ S98), Y98 (= Y100), Y99 (= Y101), F261 (= F236), S357 (= S332), G361 (≠ V336), A415 (≠ N387)
Query Sequence
>WP_035240196.1 NCBI__GCF_000745975.1:WP_035240196.1
MHKREQWGTRAGFILAAIGSAIGLGNIWRFPYVAYENGGGAFFIPYLFAMLTAGIPFLIL
EFGVGHKFKTSVPNIFRSLSGRWEWLGWWQLLVAFIISIYYVAVVGWSISYFVLAFTRGW
GADPANFFFKTYLQLSDTPFAFNGIRWPIFGAILCAWFICWGVLFSGVKKGIESASKIFM
PLLFIMVLIITARAVTLDGAKEGLNWMFKPDFSALLNFKVWIAAYGQLFFSLSIGFAIML
TYASYLPEDSDMANNGFITAFCNCGFSILCGIMVFSVLGNMAFQQGVGVDKVVSSGVGLA
FVTIPTAINSLPGPVFFGTLFFAALLFAGLSSMVSVCEVSVSALIDRFGISRKIAASLYC
GIGILCGIVFASHSGLLVLDIVDRFINNFGVLAGGLVEIIFLAWICGLDGFKGIINLTSD
FKVGTLWAFCLKIITPAVLGYMSISNLIGDIKTPYGGYPSLALFVFGWCMVGGIILLSII
INLTVQGGEK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory