SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035240982.1 NCBI__GCF_000745975.1:WP_035240982.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
32% identity, 92% coverage: 22:370/379 of query aligns to 7:376/386 of P0A9J8

query
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P0A9J8

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R11 (= R26) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R43) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K54) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E67) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (= Q102) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
34% identity, 70% coverage: 107:371/379 of query aligns to 4:275/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T274), F171 (= F276)
binding phenylalanine: N204 (≠ H300), E205 (≠ T301), L209 (= L305), D222 (≠ N318), L223 (≠ M319), T224 (≠ V320), K225 (= K321), S228 (= S324), Y238 (= Y334), F240 (= F336)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
30% identity, 70% coverage: 103:369/379 of query aligns to 5:276/282 of 6vh5D

query
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6vh5D

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binding phenylalanine: N207 (≠ H300), V208 (≠ T301), L212 (= L305), N225 (= N318), M226 (= M319), T227 (≠ V320), K228 (= K321), S231 (= S324), T241 (≠ Y334), F243 (= F336)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
34% identity, 60% coverage: 141:369/379 of query aligns to 38:274/306 of 3mwbA

query
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3mwbA

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active site: T176 (= T274), F178 (= F276)
binding phenylalanine: D205 (≠ N299), H206 (= H300), L210 (= L305), N223 (= N318), L224 (≠ M319), S225 (≠ V320), R226 (≠ K321), S229 (= S324), F241 (= F336)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
32% identity, 70% coverage: 106:369/379 of query aligns to 2:271/303 of 3mwbB

query
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3mwbB

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P

V

I

E

E

N

N

S

S

L

V

E

E

G

G

A

G

V

V

N

T

Y

A

T

T

L

L

D

D

L

A

F

I

A

A

E

T

F

G

-

Q

D

E

I

L

H

R

I

I

T

I

A

R

E

E

H

A

Y

L

E

V

P

P

V

I

S

T

H

F

D

V

L

L

L

V

S

A

I

R

T

P

G

G

-

V

E

E

L

L

K

S

D

D

V

I

K

K

T

R

V

I

Y

S

S

T

H

H

P

G

Q

H

A

A

I

W

A

A

Q

Q

C

C

K

R

N

L

W

W

L

V

K

D

K

E

H

H

L

L

P

P

H

N

A

A

T

D

V

Y

M

V

E

P

T

G

T

S

S

S

T

T

S

A

N

A

A

S

A

A

L

M

M

G

A

L

S

L

K

E

D

D

E

D

-

A

-

P

-

Y

Q

E

I

A

A

A

A

I

I

C

A

A

S

P

G

L

K

I

A
|
A

A

A

H

E

F
x
Q

Y

P

T

G

L
|
L

L

N

P

V

V

L

A

A

S

E

K

D

I

I

Q

G

D

D

R

N

A

P

G

D

N

A

I

V

T
|
T

R

R

F
|
F

L

I

V

L

I

V

G

S

-

R

-

P

G

G

N

A

H

L

P

P

E

E

R

R

T

T

G

G

N

A

D

D

K

K

T

T

S

T

I

V

M

V

-

V

-

P

F

L

A

P

T

E

N
x
D

H
|
H

T

-

P

P

G

G

A

A

L
|
L

F

M

R

E

A

I

L

L

S

D

P

Q

V

F

N

A

R

S

A

R

G

G

L

V

N
|
N

M
x
L

V
x
S

K
x
R

L
x
I

E

E

S

S

R

R

P

P

T

T

R

L

R

G

S

-

N

-

W

-

S

H

Y
|
Y

H

F

F
|
F

F

S

M

I

D

D

I

A

E

D

G

G

H

H

I

A

Q

T

D

D

E

S

I

R

V

V

F

A

Q

D

T

A

I

L

E

A

Q

G

I

L

R

H

S

R

Q

I

A

S

L

P

Y

A

L

T

K

R

V

F

L

L

G

G

S

S

Y

Y

active site: T176 (= T274), F178 (= F276)
binding magnesium ion: A155 (= A253), Q158 (≠ F256), L161 (= L259)
binding phenylalanine: D205 (≠ N299), H206 (= H300), L210 (= L305), N223 (= N318), L224 (≠ M319), S225 (≠ V320), R226 (≠ K321), I227 (≠ L322), Y236 (= Y334), F238 (= F336)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
32% identity, 59% coverage: 146:370/379 of query aligns to 44:270/278 of 7am0B

query
sites
7am0B

R

R

K

A

E

E

L

L

H

-

F

-

G

A

I

M

V

L

P

A

V

C

E

E

N

N

S

S

L

L

E

A

G

G

A

R

V

V

N

P

Y

D

T

I

L

H

D

A

L

L

F

L

A

P

E

E

F

A

D

G

I

L

H

F

I

I

T

V

A

G

E

E

H

H

Y

F

E

Q

P

R

V

V

S

E

H

H

D

C

L

L

S

G

I

I

T

P

G

G

E

S

-

T

L

L

K

A

D

D

V

A

K

R

T

R

V

I

Y

H

S

T

H

H

P

P

Q

V

A

A

I

M

A

A

Q

Q

C

V

K

R

N

G

W

I

L

I

K

T

K

E

H

-

L

L

P

G

H

L

A

D

T

P

V

V

M

V

E

E

T

F

T

-

S

D

T

T

S

A

N

G

A

A

L

E

M

M

A

V

-

R

-

E

-

W

S

G

K

R

D

K

E

E

Q

D

I

V

A

A

A

-

I

V

A

A

S

S

G

A

K

L

A

A

H

E

F

L

Y

N

T

G

L

L

L

E

P

I

V

L

A

R

S

R

K

N

I

V

Q

E

D

D

R

A

A

T

G

H

N

N

I

T

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

-

R

-

P

G

A

N

T

H

L

P

P

E

P

R

P

T

G

G

P

N

G

D

F

K

M

T

T

S

T

I

L

M

L

F

F

A

R

T

V

N
|
N

H
x
N

T
x
Q

P
|
P

G

G

A

A

L
|
L

F

Y

R

K

A

A

L

L

S

G

P

G

V

L

N

A

R

T

A

A

G

G

L

V

N
|
N

M
|
M

V
x
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

T

L

R

E

R

G

S

S

N

F

W

S

S

A

Y
x
T

H

Q

F
|
F

F

L

M

M

D

D

I

V

E

E

G

G

H

H

I

P

Q

E

D

A

E

P

I

P

V

L

F

A

Q

R

T

A

I

L

E

D

Q

E

I

L

R

S

S

F

Q

F

A

S

L

E

Y

Q

L

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: N199 (= N299), N200 (≠ H300), Q201 (≠ T301), P202 (= P302), L205 (= L305), N218 (= N318), M219 (= M319), T220 (≠ V320), R221 (≠ K321), T234 (≠ Y334), F236 (= F336)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
26% identity, 70% coverage: 105:370/379 of query aligns to 4:284/326 of 3luyA

query
sites
3luyA

R

R

T

K

I

L

G

F

F

Y

L

L

G

G

P

P

L

Q

A

G

S

T

H

F

S

T

H

H

I

Q

A

A

L

V

H

N

Y

A

F

A

R

Q

H

E

C

L

G

A

E

R

F

F

V

-

E

E

Q

P

S

Q

G

G

F

F

-

D

-

L

-

M

-

P

F

M

D

D

I

D

F

V

N

P

N

Q

I

I

E

L

R

D

K

A

E

A

L

Q

H

H

-

G

-

D

-

G

F

W

G

G

I

I

V

V

P

A

V

W

E

E

N

N

S

N

L

V

E

E

G

G

A

Y

V

V

N

V

Y

P

T

N

L

L

D

D

L

A

-

L

-

I

-

D

-

A

-

K

-

D

-

L

-

V

-

G

F

F

A

A

E

R

F

V

D

G

I

V

H

N

I

V

T

E

A

F

E

D

H

A

Y

Y

E

-

P

-

V

-

S

-

H

-

D

-

L

-

L

V

S

A

I

Q

T

G

G

A

E

D

L

P

K

A

D

E

V

A

K

R

T

I

V

A

Y

T

S

A

H

H

P

P

Q

H

A

G

I

L

A

A

Q

Q

C

C

K

K

N

R

W

F

L

I

K

A

K

E

H

H

L

-

P

-

H

-

A

-

T

R

V

L

M

S

E

T

T

Q

T

P

S

A

T

T

S

S

N

N

A

A

L

-

M

-

A

A

S

C

K

R

D

D

E

L

Q

I

I

P

A

G

A

E

I

I

A

A

S

F

G

G

K

P

A

A

-

I

-

C

A

G

H

E

F

L

Y

Y

T

D

L

I

L

T

P

R

V

I

A

G

S

T

K

A

I

I

Q

Q

D

D

R

Y

A

Q

G

G

N

A

I

A

T
|
T

R

D

F

F

L

L

V

V

I

L

G

S

G

P

N

R

-

A

-

E

-

V

-

A

-

R

-

L

-

A

H

K

P

P

E

R

R

A

T

E

G

A

N

N

-

V

-

E

-

Y

D

E

K

S

T

V

S

L

I

T

M

L

F

I

A

P

T

L

N

V

H

T

T
x
G

P

P

G

G

A

V

L
|
L

F

A

R

N

A

L

L

L

S

D

P

V

V

F

N

R

R

D

A

A

G

G

L

L

N

N

M

M

V

T

K

S

L

F

E

I

S

S

R

R

P

P

T

I

R

K

R

G

S

R

N

T

W

G

S

T

Y
|
Y

H

S

F
|
F

F

I

M

V

D

T

I

L

E

D

G

A

H

A

I

P

Q

W

D

E

E

E

I

R

V

F

F

R

Q

D

T

A

I

L

E

V

Q

E

I

I

R

A

S

E

Q

H

A

G

L

D

Y

W

L

A

K

K

V

T

L

L

G

A

S

V

Y

Y

P

P

active site: T177 (= T274)
binding 3-phenylpyruvic acid: G215 (≠ T301), L219 (= L305), Y248 (= Y334), F250 (= F336)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
29% identity, 32% coverage: 250:370/379 of query aligns to 58:186/194 of 7alzA

query
sites
7alzA

S

A

G

G

K

R

A

V

A

P

H

D

F

I

Y

H

T

A

L

L

P

P

V

E

A

A

S

G

K

L

I

F

Q

I

-

V

-

G

-

E

-

H

-

F

D

Q

R

R

A

V

G

H

N

N

I

T

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

-

R

-

P

G

A

N

T

H

L

P

P

E

P

R

P

T

G

G

P

N

G

D

F

K

M

T

T

S

T

I

L

M

L

F

F

A

R

T

V

N

N

H

N

T
x
Q

P

P

G

G

A

A

L
|
L

F

Y

R

K

A

A

L

L

S

G

P

G

V

L

N

A

R

T

A

A

G

G

L

V

N
|
N

M
|
M

V
x
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

T

L

R

E

R

G

S

S

N

F

W

S

S

A

Y
x
T

H

Q

F
|
F

F

L

M

M

D

D

I

V

E

E

G

G

H

H

I

P

Q

E

D

A

E

P

I

P

V

L

F

A

Q

R

T

A

I

L

E

D

Q

E

I

L

R

S

S

F

Q

F

A

S

L

E

Y

Q

L

Q

K

E

V

I

L

L

G

G

S

V

Y

Y

P

P

binding phenylalanine: Q117 (≠ T301), L121 (= L305), N134 (= N318), M135 (= M319), T136 (≠ V320), R137 (≠ K321), T150 (≠ Y334), F152 (= F336)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
35% identity, 22% coverage: 22:103/379 of query aligns to 4:85/352 of 5j6fA

query
sites
5j6fA

L

L

S

D

P

E

L

L

R

R

G

A

E

R

I

V

D

D

R

E

I

I

D

N

D

L

R

Q

I

L

L

L

E

K

L

L

I

I

N

N

Q

E

R
|
R

L

G

E

R

I

L

G

V

K

Q

K

E

V
x
I

G

G

E

K

I

I

K
|
K

K

E

Q

A

K

Q

G

G

S

T

Q

H

I

R

L
x
Y

D
|
D

R

P

S

V

R
|
R

E

E

R

R

M

K

V
x
M

I

L

E

D

R

L

L

I

S

S

R

E

T

H

N

N

P

D

G

G

P

P

A

F

D

E

A

T

S

S

L

T

I

L

Q

Q

Y

H

V

I

F

F

N

K

V

E

I

I

I

F

T

K

A

A

T

A

R

L

E

E

I

L

Q

Q

K

E

binding prephenic acid: R25 (= R43), I32 (≠ V50), K36 (= K54), Y44 (≠ L62), D45 (= D63), R48 (= R66), M52 (≠ V70)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
35% identity, 22% coverage: 20:103/379 of query aligns to 4:87/358 of P39912

query
sites
P39912

S

T

S

E

L

L

S

E

P

L

L

L

R

R

G

Q

E

K

I

A

D

D

R

E

I

L

D

N

D

L

R

Q

I

I

L

L

E

K

L

L

I

I

N

N

Q

E

R

R

L

G

E

N

I

V

G

V

K

K

K

E

V

I

G

G

E

K

I

A

K

K

K

E

Q

A

K

Q

G

G

S

V

Q

N

I

R

L

F

D

D

R

P

S

V

R

R

E

E

R

R

M

T

V

M

I

L

E

N

R

N

L

I

S

I

R

E

T

N

N

N

P

D

G

G

P

P

A

F

D

E

A

N

S

S

L

T

I

I

Q

Q

Y

H

V

I

F

F

N

K

V

E

I

I

I

F

T

K

A

A

T

G

R

L

E

E

I

L

Q

Q

K

E

Sites not aligning to the query:

2 modified: Phosphoserine

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
35% identity, 22% coverage: 20:103/379 of query aligns to 3:86/87 of 5gmuB

query
sites
5gmuB

S

T

S

E

L

L

S

E

P

L

L

L

R

R

G

Q

E

K

I

A

D

D

R

E

I

L

D

N

D

L

R

Q

I

I

L

L

E

K

L

L

I

I

N

N

Q

E

R
|
R

L

G

E

N

I

V

G

V

K

K

K

E

V

I

G

G

E

K

I

A

K

K

K

E

Q

A

K

Q

G

G

S

V

Q

N

I
x
R

L
x
F

D
|
D

R

P

S

V

R

R

E
|
E

R

R

M

T

V

M

I

L

E

N

R

N

L

I

S

I

R

E

T

N

N

N

P

D

G

G

P

P

A

F

D

E

A

N

S

S

L

T

I

I

Q

Q

Y

H

V

I

F

F

N

K

V

E

I

I

I
x
F

T
x
K

A

A

T

G

R
x
L

E

E

I

L

Q
|
Q

K

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R43), R44 (≠ I61), F45 (≠ L62), D46 (= D63), E50 (= E67), F78 (≠ I95), K79 (≠ T96), L82 (≠ R99), Q85 (= Q102)

5ckxD Non-covalent complex of dahp synthase and chorismate mutase from mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine (see paper)
40% identity, 17% coverage: 22:86/379 of query aligns to 3:67/79 of 5ckxD

query
sites
5ckxD

L

I

S

D

P

T

L

L

R

R

G

E

E

E

I

I

D

D

R

R

I

L

D

D

D

A

R

E

I

I

L

L

E

A

L

L

I

V

N

K

Q

R

R
|
R

L

A

E

E

I

V

G
x
S

K

K

K

A

V
x
I

G

G

E

K

I

A

K
x
R

K

M

Q

A

K

S

G

G

S

G

Q

T

I

R

L

L

D

V

R

H

S

S

R
|
R

E
|
E

R

M

M

K

V

V

I

I

E

E

R

R

L

Y

S

S

R

E

T

L

N

G

P

P

G

D

P

G

A

K

D

D

A

L

S

A

L

I

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R24 (= R43), S28 (≠ G47), I31 (≠ V50), R35 (≠ K54), R47 (= R66), E48 (= E67)

Sites not aligning to the query:

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: 70

P9WIC1 Intracellular chorismate mutase; CM; EC 5.4.99.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 19% coverage: 16:86/379 of query aligns to 23:93/105 of P9WIC1

query
sites
P9WIC1

S

S

D

Q

D

P

L

V

S

P

S

E

L

I

S

D

P

T

L

L

R

R

G

E

E

E

I

I

D

D

R

R

I

L

D

D

D

A

R

E

I

I

L

L

E

A

L

L

I

V

N

K

Q

R

R

R

L

A

E

E

I

V

G

S

K

K

K

A

V

I

G

G

E

K

I

A

K
x
R

K

M

Q

A

K

S

G

G

S

G

Q

T

I

R

L

L

D

V

R

H

S

S

R

R

E

E

R

M

M

K

V

V

I

I

E

E

R

R

L

Y

S

S

R

E

T

L

N

G

P

P

G

D

P

G

A

K

D

D

A

L

S

A

L

I

R61 (≠ K54) Important for catalysis; mutation to K: It is catalytically catastrophic, but strongly activated by AroG.

Sites not aligning to the query:

101 Important for activation via AroG; G→A: The catalytic efficiency and the affinity are 5 and 3-fold lower than the wild-type. The activation by AroG is 10-fold lower than the wild-type.
102 Important for activation via AroG; R→A: The catalytic efficiency and the affinity are slightly modified. The activation by AroG is 2-fold lower than the wild-type.
103 Important for activation via AroG; L→A: The catalytic efficiency and the affinity are identical to the wild-type. The activation by AroG is 10-fold lower than the wild type.

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
32% identity, 21% coverage: 25:103/379 of query aligns to 2:75/345 of 3nvtA

query
sites
3nvtA

L

L

R

R

G

T

E

Q

I

V

D

D

R

Q

I

L

D

N

D

I

R

D

I

L

L

L

E

E

L

L

I

I

N

S

Q

K

R

R

L

A

E

N

I

L

G

V

K

Q

K

E

V

I

G

G

E

K

I

I

K

K

K

G

Q

R

K

-

G

-

S

-

Q

-

I

-

L

F

D

D

R

P

S

L

R

R

E

E

R

R

M

E

V

M

I

L

E

N

R

T

L

I

S

L

R

A

T

A

N

N

P

E

G

G

P

P

A

F

D

E

A

D

S

S

L

T

I

V

Q

Q

Y

K

V

L

F

F

N

K

V

E

I

I

I

F

T

K

A

A

T

G

R

L

E

E

I

L

Q

Q

K

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

Query Sequence

>WP_035240982.1 NCBI__GCF_000745975.1:WP_035240982.1
MTNREVREKDNQKQTSDDLSSLSPLRGEIDRIDDRILELINQRLEIGKKVGEIKKQKGSQ
ILDRSRERMVIERLSRTNPGPADASLIQYVFNVIITATREIQKPRTIGFLGPLASHSHIA
ALHYFRHCGEFVEQSGFFDIFNNIERKELHFGIVPVENSLEGAVNYTLDLFAEFDIHITA
EHYEPVSHDLLSITGELKDVKTVYSHPQAIAQCKNWLKKHLPHATVMETTSTSNAALMAS
KDEQIAAIASGKAAHFYTLLPVASKIQDRAGNITRFLVIGGNHPERTGNDKTSIMFATNH
TPGALFRALSPVNRAGLNMVKLESRPTRRSNWSYHFFMDIEGHIQDEIVFQTIEQIRSQA
LYLKVLGSYPIFSKPEILQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory