SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035241251.1 NCBI__GCF_000745975.1:WP_035241251.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6ih6A Phosphite dehydrogenase mutant i151r/p176r/m207a from ralstonia sp. 4506 in complex with non-natural cofactor nicotinamide cytosine dinucleotide
54% identity, 99% coverage: 3:329/330 of query aligns to 2:328/330 of 6ih6A

query
sites
6ih6A

K

K

P

P

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K

I

V

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L

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T

H

H

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W

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Y

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L

L

S

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E

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Y

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A

E

D

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V

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N

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K

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I

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E

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D

A

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D

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A

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M

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A

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F

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M

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D

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A

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L

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K

G

G

Y

Y

D

D

N

N

F

F

D

D

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V

D

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A

A

C

C

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T

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K

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G

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D

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A

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binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: T104 (= T105), R151 (≠ L152), G154 (= G155), A155 (≠ E156), V156 (= V157), D175 (= D176), A207 (= A208), V208 (≠ L209), P209 (= P210), T214 (= T215), A235 (≠ V236), C236 (≠ G237), R237 (= R238)

4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
48% identity, 99% coverage: 4:330/330 of query aligns to 3:328/329 of 4e5kA

query
sites
4e5kA

P

P

K

K

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L

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T

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H

W

R

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H

E

E

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A

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P

Y

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C

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E

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M

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M

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Y

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D

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N

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x
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F

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N

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T

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I

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L

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D

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K

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C

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K

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Y

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V

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x
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D

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A

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D

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W

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R

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A

L

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R

H

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N

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T

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L

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F

L

-

T

T

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H
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H

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S

S

A

A

V

V

E

R

S

A

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V

R

R

K

L

N

E

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C

A

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N

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A

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A

G

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K

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L

active site: L100 (= L101), R237 (= R238), D261 (= D262), E266 (= E267), H292 (= H294)
binding nicotinamide-adenine-dinucleotide: K76 (= K77), G77 (= G78), L100 (= L101), T104 (= T105), G152 (= G153), G154 (= G155), A155 (≠ E156), I156 (≠ V157), H174 (≠ Y175), E175 (≠ D176), A176 (≠ T177), A207 (= A208), L208 (= L209), P209 (= P210), P235 (≠ V236), C236 (≠ G237), R237 (= R238), D261 (= D262), H292 (= H294), G294 (= G296)
binding sulfite ion: M53 (= M54), L75 (= L76), K76 (= K77), G77 (= G78), L100 (= L101), R237 (= R238), H292 (= H294)

4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
48% identity, 99% coverage: 4:330/330 of query aligns to 3:328/329 of 4e5mA

query
sites
4e5mA

P

P

K

K

I

L

L

V

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T

T

H

H

W

R

V

V

H

H

E

E

I

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Y

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A

E

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Y

H

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C

E

E

V

L

V

I

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T

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N

Q

Q

T

T

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D

Q

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S

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M

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K

T

K

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E

R

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L

A

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R

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C

E

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N

D

A

A

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A

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M

M

A

A

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F

M

M

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P

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K

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D

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F

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C

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V

I

G

G

A

C

A

A

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L

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K

G

G

Y

F

D

D

N

N

F

F

D

D

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V

D

D

A

A

C

C

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T

T

A

K

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G

G

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W

F

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I

F

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P

N

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H

L

L
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L

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T

A

A

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E

L

L

A

A

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I

G

G

H

L

L

A

I

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A

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A

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A

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F

F

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F

Y

Y

G

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C

A

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x
R

C

K

K

A

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L

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Q

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E

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L

Y

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A

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F

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I

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A

L
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L

P
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P

L

L

N

N

P

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D

T
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T

I

L

H

H

V

L

I

V

D

N

I

A

Q

E

A

L

L

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A

K

L

C

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K

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K

P

G

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Y

L

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N

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x
P

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x
C

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R

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G

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D

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K

A

A

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A

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R

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G

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L

A

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G

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Y

Y

A

A

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D

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V

F

F

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E
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E

D

D

W

W

A

A

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A

G

D

R

R

P

P

Q

Q

A

Q

I

I

P

D

A

P

E

A

L

L

T

L

D

A

R

H

L

P

D

N

T

T

T

L

F

F

L

-

T

T

P

P

H
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H

L

I

G
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G

S

S

A

A

V

V

E

R

S

A

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V

R

R

K

L

N

E

I

I

A

E

M

R

E

C

A

A

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Q

N

N

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I

V

L

E

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A

A

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L

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active site: L100 (= L101), R237 (= R238), D261 (= D262), E266 (= E267), H292 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (= K77), L100 (= L101), T104 (= T105), G154 (= G155), A155 (≠ E156), I156 (≠ V157), R176 (≠ T177), L208 (= L209), P209 (= P210), T214 (= T215), P235 (≠ V236), C236 (≠ G237), R237 (= R238), H292 (= H294), G294 (= G296)

4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
48% identity, 99% coverage: 4:330/330 of query aligns to 3:328/332 of 4e5pA

query
sites
4e5pA

P

P

K

K

I

L

L

V

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I

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T

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H

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R

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H

H

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N

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L

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E

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D

D

F

F

I

I

V

L

A

A

L
|
L

P
|
P

L

L

N

N

P

A

D

D

T
|
T

I

L

H

H

V

L

I

V

D

N

I

A

Q

E

A

L

L

L

D

A

K

L

C

V

K

R

K

P

G

G

V

A

Y

L

I

L

V

V

N

N

V
x
P

G
x
C

R
|
R

G

G

S

S

S

V

V

V

D

D

E

E

K

A

A

A

V

V

A

L

Q

A

G

A

L

L

E

E

K

R

G

G

Q

Q

L

L

A

G

G

G

Y

Y

A

A

D
|
D

V

V

F

F

E

E

F

M

E
|
E

D

D

W

W

A

A

R

R

T

A

G

D

R

R

P

P

Q

Q

A

Q

I

I

P

D

A

P

E

A

L

L

T

L

D

A

R

H

L

P

D

N

T

T

T

L

F

F

L

-

T

T

P

P

H
|
H

L

I

G

G

S

S

A

A

V

V

E

R

S

A

V

V

R

R

K

L

N

E

I

I

A

E

M

R

E

C

A

A

V

Q

N

N

I

I

V

L

E

Q

A

A

L

L

Q

A

G

G

K

E

R

R

P

P

H

I

G

N

A

A

I

V

N

N

S

R

I

L

active site: L100 (= L101), R237 (= R238), D261 (= D262), E266 (= E267), H292 (= H294)
binding nicotinamide-adenine-dinucleotide: K76 (= K77), L100 (= L101), T104 (= T105), G154 (= G155), A155 (≠ E156), I156 (≠ V157), A175 (≠ D176), R176 (≠ T177), L208 (= L209), P209 (= P210), T214 (= T215), P235 (≠ V236), C236 (≠ G237), R237 (= R238), H292 (= H294)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
33% identity, 99% coverage: 3:329/330 of query aligns to 2:323/334 of 5aovA

query
sites
5aovA

K

K

P

P

K

K

I

V

L

F

I

I

T

T

H

R

W

A

V

I

H

P

E

E

E

N

I

G

I

I

E

N

Y

M

L

L

S

E

E

E

Y

E

G

F

E

E

V

V

V

E

T

V

N

W

Q

E

T

E

R

E

Q

R

S

E

M

I

K

P

K

R

G

E

R

K

L

L

W

L

A

E

E

K

I

V

E

K

N

D

A

V

V

D

A

A

I

L

M

V

A

T

F
x
M

M
x
L

P

S

D

E

S

R

I

I

D

D

K

Q

E

E

F

V

L

F

S

E

H

N

C

A

N

P

K

R

L

L

K

R

I

I

V

V

G

A

A

N

A
x
Y

L
x
A

K
x
V

G
|
G

Y

Y

D

D

N

N

F

I

D

D

V

V

D

E

A

E

C

A

T

T

T

R

K

R

G

G

V

I

W

Y

F

V

S

T

I

N

V

T

P

P

N

D

H

V

L
|
L

T

T

T

N

P

A

T
|
T

A

A

E

D

L

H

A

A

V

F

G

A

H

L

L

L

I

L

A

A

I

T

G

A

R

R

N

H

M

V

Q

V

A

K

G

G

D

D

R

K

L

F

I

V

R

R

N

S

G

G

H

E

F

W

P

K

-

R

-

K

-

G

-

I

G

A

W

W

R

H

P

P

V

K

L

W

Y

F

-

L

G

G

R

Y

G

E

L

L

A

Y

G

G

S

K

T

T

I

I

G

G

I

I

L

V

G

G

M
x
F

G
|
G

E
x
R

V
x
I

G

G

R

Q

A

A

I

I

A

A

K

R

L

R

L

A

M

K

S

G

F

F

S

N

C

M

S

R

C

I

L

L

Y

Y

D
x
S

T
x
R

C

T

K

R

L

K

E

S

K

Q

S

A

R

-

E

E

Q

K

E

E

L

L

N

G

A

A

Y

E

S

Y

S

R

S

P

L

L

E

E

D

E

I

V

Y

L

R

K

K

E

S

S

D

D

F

F

I

V

V

I

V

L

A
|
A

L
x
V

P
|
P

L

L

N

T

P

K

D

E

T
|
T

I

M

H

Y

V

M

I

I

D

N

I

E

Q

E

A

R

L

L

D

K

K

L

C

M

K

K

K

P

G

T

V

A

Y

I

I

L

V

V

N

N

V
x
I

G
x
A

R
|
R

G

G

S

K

S

V

V

V

D

D

E

T

K

K

A

A

V

L

A

I

Q

K

G

A

L

L

E

K

K

E

G

G

Q

W

L

I

A

A

G

G

Y

A

A

G

A

L

D
|
D

V

V

F

F

E

E

F

E

E
|
E

D

P

W

Y

A

Y

R

N

T

-

G

-

R

-

P

-

Q

-

A

-

I

-

P

-

A

-

E

E

L

E

T

L

D

F

R

S

L

L

D

D

T

N

T

V

F

V

L

L

T

T

P

P

H
|
H

L

I

G
|
G

S

S

A

A

V

T

E

F

S

E

V

A

R

R

K

E

N

A

I

M

A

A

M

E

E

L

A

V

A

A

V

R

N

N

I

L

V

I

E

A

A

F

L

K

Q

R

G

G

K

E

R

I

P

P

H

P

G

T

A

L

I

V

N

N

S

K

active site: L100 (= L101), R241 (= R238), D265 (= D262), E270 (= E267), H288 (= H294)
binding glyoxylic acid: M52 (≠ F53), L53 (≠ M54), L53 (≠ M54), Y74 (≠ A75), A75 (≠ L76), V76 (≠ K77), G77 (= G78), R241 (= R238), H288 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ K77), T104 (= T105), F158 (≠ M154), G159 (= G155), R160 (≠ E156), I161 (≠ V157), S180 (≠ D176), R181 (≠ T177), A211 (= A208), V212 (≠ L209), P213 (= P210), T218 (= T215), I239 (≠ V236), A240 (≠ G237), R241 (= R238), H288 (= H294), G290 (= G296)

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
34% identity, 99% coverage: 3:328/330 of query aligns to 1:321/332 of 6biiA

query
sites
6biiA

K

K

P

P

K

K

I

V

L

L

I

I

T

T

H

R

W

A

V

I

H

P

E

E

E

N

I

G

I

I

E

E

Y

L

L

L

S

R

E

E

Y

H

G

F

E

E

V

V

V

E

T

V

N

W

Q

E

T

H

R

E

Q

H

S

E

M

I

K

P

K

R

G

E

R

V

L

L

W

L

A

E

E

K

I

V

E

K

N

D

A

V

V

D

A

A

I

L

M

V

A

T

F

M

M

L

P

S

D

E

S

K

I

I

D

D

K

R

E

E

F

V

L

F

S

D

H

A

C

A

N

P

K

R

L

L

K

R

I

I

V

V

G

A

A

N

A

Y

L

A

K
x
V

G

G

Y

Y

D

D

N

N

F

I

D

D

V

I

D

E

A

E

C

A

T

T

T

K

K

R

G

G

V

I

W

Y

F

V

S

T

I

N

V

T

P

P

N

D

H

V

L
|
L

T

T

T

D

P

A

T
|
T

A

A

E

D

L

L

A

A

V

W

G

A

H

L

L

L

I

L

A

A

I

A

G

A

R

R

N

H

M

V

Q

V

A

K

G

G

D

D

R

K

L

F

I

V

R

R

N

S

G

G

H

E

F

W

P

K

-

R

-

G

-

I

G

A

W

W

R

H

P

P

V

K

L

M

Y

F

-

L

G

G

R

Y

G

D

L

V

A

Y

G

G

S

K

T

T

I

I

G

G

I

I

L

V

G
|
G

M
x
F

G
|
G

E
x
R

V
x
I

G

G

R

Q

A

A

I

I

A

A

K

K

L

R

L

A

M

K

S

G

F

F

S

G

C

M

S

R

C

I

L

L

Y

Y

Y

T

D
x
A

T
x
R

C
x
S

K

R

L
x
K

E

P

K

E

S

A

R

-

E

E

Q

K

E

E

L

L

N

G

A

A

Y

E

S

F

S

K

S

P

L

L

E

E

D

E

I

L

Y

L

R

R

K

E

S

S

D

D

F

F

I

V

V

V

V

L

A

A

L
x
V

P
|
P

L

L

N

T

P

K

D
x
E

T
|
T

I

Y

H

H

V

M

I

I

D

N

I

E

Q

E

A

R

L

L

D

R

K

L

C

M

K

K

K

P

G

T

V

A

Y

V

I

L

V

V

N

N

V
|
V

G
x
A

R
|
R

G

G

S

K

S

V

V

V

D

D

E

T

K

K

A

A

V

L

A

I

Q

R

G

A

L

L

E

K

K

E

G

G

Q

W

L

I

A

A

G

A

Y

A

A

G

A

L

D
|
D

V

V

F

F

E

E

F

E

E
|
E

D

P

W

Y

A

Y

R

-

T

-

G

-

R

-

P

-

Q

-

A

-

I

-

P

-

A

D

E

E

L

E

T

L

D

F

R

A

L

L

D

D

T

N

T

V

F

V

L

L

T

T

P

P

H
|
H

L

I

G
|
G

S

S

A

A

V

T

E

F

S

G

V

A

R

R

K

E

N

G

I

M

A

A

M

E

E

L

A

V

A

A

V

K

N

N

I

L

V

I

E

A

A

F

L

K

Q

N

G

G

K

E

R

V

P

P

H

P

G

T

A

L

I

V

N

N

active site: L99 (= L101), R240 (= R238), D264 (= D262), E269 (= E267), H287 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ K77), T103 (= T105), G156 (= G153), F157 (≠ M154), G158 (= G155), R159 (≠ E156), I160 (≠ V157), A179 (≠ D176), R180 (≠ T177), S181 (≠ C178), K183 (≠ L180), V211 (≠ L209), P212 (= P210), E216 (≠ D214), T217 (= T215), V238 (= V236), A239 (≠ G237), R240 (= R238), D264 (= D262), H287 (= H294), G289 (= G296)

O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 99% coverage: 3:328/330 of query aligns to 2:322/334 of O58320

query
sites
O58320

K

K

P

P

K

K

I

V

L

F

I

I

T

T

H

R

W

E

V

I

H

P

E

E

E

V

I

G

I

I

E

K

Y

M

L

L

S

E

E

D

Y

E

G

F

E

E

V

V

V

E

T

V

N

W

Q

G

T

D

R

E

Q

K

S

E

M

I

K

P

K

R

G

E

R

I

L

L

W

L

A

K

E

K

I

V

E

K

N

E

A

V

V

D

A

A

I

L

M

V

A

T

F

M

M

L

P

S

D

E

S

R

I

I

D

D

K

K

E

E

F

V

L

F

S

E

H

N

C

A

N

P

K

K

L

L

K

R

I

I

V

V

G

A

A

N

A

Y

L

A

K

V

G

G

Y

Y

D

D

N

N

F

I

D

D

V

I

D

E

A

E

C

A

T

T

T

K

K

R

G

G

V

I

W

Y

F

V

S

T

I

N

V

T

P

P

N

D

H

V

L

L

T

T

T

D

P

A

T

T

A

A

E

D

L

L

A

A

V

F

G

A

H

L

L

L

I

L

A

A

I

T

G

A

R

R

N

H

M

V

Q

V

A

K

G

G

D

D

R

R

L

F

I

V

R

R

N

S

G

G

H

E

F

W

P

K

-

K

-

R

-

G

-

V

G

A

W

W

R

H

P

P

V

K

L

W

Y

F

-

L

G

G

R

Y

G

D

L

V

A

Y

G

G

S

K

T

T

I

I

G

G

I

I

L

I

G

G

M
x
L

G
|
G

E
x
R

V
x
I

G

G

R

Q

A

A

I

I

A

A

K

K

L

R

L

A

M

K

S

G

F

F

S

N

C

M

S

R

C

I

L

L

Y

Y

D
x
S

T
x
R

C
x
T

K

R

L

K

E

E

K

E

S

V

R

-

E

E

Q

R

E

E

L

L

N

N

A

A

Y

E

S

F

S

K

S

P

L

L

E

E

D

D

I

L

Y

L

R

R

K

E

S

S

D

D

F

F

I

V

V

V

V

L

A

A

L

V

P

P

L

L

N

T

P

R

D

E

T

T

I

Y

H

H

V

L

I

I

D

N

I

E

Q

E

A

R

L

L

D

K

K

L

C

M

K

K

G

T

V

A

Y

I

I

L

V

I

N

N

V
x
I

G
x
A

R
|
R

G

G

S

K

S

V

V

V

D

D

E

T

K

N

A

A

V

L

A

V

Q

K

G

A

L

L

E

K

K

E

G

G

Q

W

L

I

A

A

G

G

Y

A

A

G

A

L

D

D

V

V

F

F

E

E

F

E

E

E

D

P

W

Y

A

Y

R

N

T

-

G

-

R

-

P

-

Q

-

A

-

I

-

P

-

A

-

E

E

L

E

T

L

D

F

R

K

L

L

D

D

T

N

T

V

F

V

L

L

T

T

P

P

H
|
H

L
x
I

G
|
G

S

S

A

A

V

S

E

F

S

G

V

A

R

R

K

E

N

G

I

M

A

A

M

E

E

L

A

V

A

A

V

K

N

N

I

L

V

I

E

A

A

F

L

K

Q

R

G

G

K

E

R

I

P

P

H

P

G

T

A

L

I

V

N

N

LGRI 158:161 (≠ MGEV 154:157) binding NADP(+)
SRT 180:182 (≠ DTC 176:178) binding NADP(+)
IAR 239:241 (≠ VGR 236:238) binding NADP(+)
HIG 288:290 (≠ HLG 294:296) binding NADP(+)

2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 99% coverage: 3:328/330 of query aligns to 2:322/333 of 2dbqA

query
sites
2dbqA

K

K

P

P

K

K

I

V

L

F

I

I

T

T

H

R

W

E

V

I

H

P

E

E

E

V

I

G

I

I

E

K

Y

M

L

L

S

E

E

D

Y

E

G

F

E

E

V

V

V

E

T

V

N

W

Q

G

T

D

R

E

Q

K

S

E

M

I

K

P

K

R

G

E

R

I

L

L

W

L

A

K

E

K

I

V

E

K

N

E

A

V

V

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A

A

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D

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K

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E

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C

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x
V

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G

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Y

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D

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x
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R

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E

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G

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A

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A

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N

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N

active site: L100 (= L101), R241 (= R238), D265 (= D262), E270 (= E267), H288 (= H294)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ K77), T104 (= T105), L158 (≠ M154), G159 (= G155), R160 (≠ E156), I161 (≠ V157), S180 (≠ D176), R181 (≠ T177), T182 (≠ C178), A211 (= A208), V212 (≠ L209), P213 (= P210), T218 (= T215), I239 (≠ V236), A240 (≠ G237), R241 (= R238), D265 (= D262), H288 (= H294), G290 (= G296)

O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
30% identity, 98% coverage: 5:329/330 of query aligns to 8:318/533 of O43175

query
sites
O43175

K

K

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G

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x
T

G

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A

A

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x
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A

T78 (≠ K77) binding NAD(+)
R135 (= R136) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
RI 155:156 (≠ EV 156:157) binding NAD(+)
D175 (= D176) binding NAD(+)
T207 (≠ L209) binding NAD(+)
CAR 234:236 (≠ VGR 236:238) binding NAD(+)
D260 (= D262) binding NAD(+)
V261 (= V263) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
HLGA 283:286 (≠ HLGS 294:297) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987

6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
30% identity, 96% coverage: 5:320/330 of query aligns to 4:305/305 of 6plfA

query
sites
6plfA

K

K

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L

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C

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K

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N

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A

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A

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A

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G

binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: Y170 (= Y175), D171 (= D176), P172 (≠ T177), L189 (= L195), H202 (≠ A208), T203 (≠ L209), P204 (= P210), L212 (≠ V218)

7dkmA Phgdh covalently linked to oridonin (see paper)
30% identity, 95% coverage: 5:319/330 of query aligns to 4:304/306 of 7dkmA

query
sites
7dkmA

K

K

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A

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G

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x
T

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A

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T

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x
A

A

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x
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C

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K

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G

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C

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x
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R

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G

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I

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D

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K

G

A

A

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A

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G

A

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G

Q

Q

L

C

A

A

G

G

Y

A

A

A

A

L

D

D

V

V

F

F

E

T

F

E

E

E

D

-

W

-

A

-

R

-

T

P

G

P

R

R

P

D

Q

R

A

A

I

-

P

-

A

-

E

-

L

L

T

V

D

D

R

H

L

-

D

E

T

N

T

V

F

I

L

S

T

C

P

P

H
|
H

L

L

G
|
G

S

A

A

S

V

T

E

K

S

E

V

A

R

Q

K

S

N

R

I

C

A

G

M
x
E

E

E

A

I

A

A

V

V

N

Q

I

F

V

V

E

D

A

M

L

V

Q

K

binding nicotinamide-adenine-dinucleotide: T74 (≠ K77), A102 (≠ T105), G148 (= G153), R151 (≠ E156), I152 (≠ V157), Y170 (= Y175), D171 (= D176), P172 (≠ T177), I173 (≠ C178), H202 (≠ A208), T203 (≠ L209), P204 (= P210), T209 (= T215), C230 (≠ V236), A231 (≠ G237), R232 (= R238), H279 (= H294), G281 (= G296)
binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ E15), K17 (≠ E18), I18 (≠ Y19), E293 (≠ M308)

6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
30% identity, 95% coverage: 5:319/330 of query aligns to 3:303/303 of 6plgA

query
sites
6plgA

K

K

I

V

L

L

I

I

T

S

H

D

W

S

V

L

H

D

E

P

E

C

I

C

I

R

E

K

Y

I

L

L

S

Q

E

D

Y

G

G

G

E

L

V

Q

V

V

T

V

N

E

Q

-

T

-

R

K

Q

Q

S

N

M

L

K

S

K

K

G

E

R

E

L

L

W

I

A

A

E

E

I

L

E

Q

N

D

A

C

V

E

A

G

I

L

M

I

A

V

F

R

M

S

P

A

D

T

S

K

I

V

D

T

K

A

E

D

F

V

L

I

S

N

H

A

C

A

N

E

K

K

L

L

K

Q

I

V

V

V

G

G

A

R

A

A

L

G

K

T

G

G

Y

V

D

D

N

N

F

V

D

D

V

L

D

E

A

A

C

A

T

T

T

R

K

K

G

G

V

I

W

L

F

V

S

M

I

N

V

T

P

P

N

N

H

G

L

N

T

S

T

L

P

S

T

A

A

A

E

E

L

L

A

T

V

C

G

G

H

M

L

I

I

M

A

C

I

L

G

A

R

R

N

Q

M

I

Q

P

A

Q

G

A

D

T

R

A

L

S

I

M

R

K

N

D

G

G

H

K

F

W

P

E

G

-

W

-

R

R

P

K

V

K

L

F

Y

M

G

G

R

T

G

E

L

L

A

N

G

G

S

K

T

T

I

L

G

G

I

I

L

L

G

G

M

L

G
|
G

E
x
R

V

I

G

G

R

R

A

E

I

V

A

A

K

T

L

R

L

M

M

Q

S

S

F

F

S

G

C

M

S

K

C

T

L

I

Y

G

Y

Y

D
|
D

T
x
P

C
x
I

K

-

L
x
I

E

S

K

P

S

E

R

V

E

S

Q

A

E

S

L

F

N

G

A

V

Y

Q

S

Q

S

L

S

P

L

L

E

E

D

E

I

I

Y

W

R

P

K

L

S

C

D

D

F

F

I

I

V

T

V

V

A
x
H

L
x
T

P

P

L

L

N
x
L

P

P

D

S

T

T

I

T

H

G

V

L

I

L

D

N

I

D

Q

N

A

T

L

F

D

A

K

Q

C

C

K

K

G

G

V

V

Y

R

I

V

V

V

N

N

V

C

G

A

R

R

G

G

S

G

S

I

V

V

D

D

E

E

K

G

A

A

V

L

A

L

Q

R

G

A

L

L

E

Q

K

S

G

G

Q

Q

L

C

A

A

G

G

Y

A

A

A

A

L

D

D

V

V

F

F

E

T

F

E

E

E

D

-

W

-

A

-

R

-

T

P

G

P

R

R

P

D

Q

R

A

A

I

-

P

-

A

-

E

-

L

L

T

V

D

D

R

H

L

-

D

E

T

N

T

V

F

I

L

S

T

C

P

P

H

H

L

L

G

G

S

A

A

S

V

T

E

K

S

E

V

A

R

Q

K

S

N

R

I

C

A

G

M

E

E

E

A

I

A

A

V

V

N

Q

I

F

V

V

E

D

A

M

L

V

Q

K

binding (2S)-(4-{3-[(4,5-dichloro-1-methyl-1H-indole-2-carbonyl)amino]oxetan-3-yl}phenyl)(pyridin-3-yl)acetic acid: G149 (= G155), R150 (≠ E156), D170 (= D176), P171 (≠ T177), I172 (≠ C178), I173 (≠ L180), H201 (≠ A208), T202 (≠ L209), L205 (≠ N212)

6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
30% identity, 95% coverage: 5:316/330 of query aligns to 2:299/299 of 6cwaA

query
sites
6cwaA

K

K

I

V

L

L

I

I

T

S

H

D

W

S

V

L

H

D

E

P

E

C

I

C

I

R

E

K

Y

I

L

L

S

Q

E

D

Y

G

G

G

E

L

V

Q

V

V

T

V

N

E

Q

-

T

-

R

K

Q

Q

S

N

M

L

K

S

K

K

G

E

R

E

L

L

W

I

A

A

E

E

I

L

E

Q

N

D

A

C

V

E

A

G

I

L

M

I

A

V

F

R

M

S

P

A

D

T

S

K

I

V

D

T

K

A

E

D

F

V

L

I

S

N

H

A

C

A

N

E

K

K

L

L

K

Q

I

V

V

V

G

G

A

R

A

A

L

G

K

T

G

G

Y

V

D

D

N

N

F

V

D

D

V

L

D

E

A

A

C

A

T

T

T

R

K

K

G

G

V

I

W

L

F

V

S

M

I

N

V

T

P

P

N

N

H

G

L
x
N

T

S

T

L

P

S

T
x
A

A

A

E

E

L

L

A

T

V

C

G

G

H

M

L

I

I

M

A

C

I

L

G

A

R

R

N

Q

M

I

Q

P

A

Q

G

A

D

T

R

A

L

S

I

M

R

K

N

D

G

G

H

K

F

W

P

E

G

-

W

-

R

R

P

K

V

K

L

F

Y

M

G

G

R

T

G

E

L

L

A

N

G

G

S

K

T

T

I

L

G

G

I

I

L

L

G

G

M

L

G

G

E
x
R

V
x
I

G

G

R

R

A

E

I

V

A

A

K

T

L

R

L

M

M

Q

S

S

F

F

S

G

C

M

S

K

C

T

L

I

Y

G

Y
|
Y

D
|
D

T
x
P

C
x
I

K

-

L

I

E

S

K

P

S

E

R

V

E

S

Q

A

E

S

L

F

N

G

A

V

Y

Q

S

Q

S

L

S

P

L

L

E

E

D

E

I

I

Y

W

R

P

K

L

S

C

D

D

F

F

I

I

V

T

V

V

A
x
H

L
x
T

P
|
P

L

L

N

L

P

P

D

S

T
|
T

I

T

H

G

V

L

I

L

D

N

I

D

Q

N

A

T

L

F

D

A

K

Q

C

C

K

K

G

G

V

V

Y

R

I

V

V

V

N

N

V
x
C

G
x
A

R
|
R

G

G

S

G

S

I

V

V

D

D

E

E

K

G

A

A

V

L

A

L

Q

R

G

A

L

L

E

Q

K

S

G

G

Q

Q

L

C

A

A

G

G

Y

A

A

A

A

L

D

D

V

V

F

F

E

T

F

E

E

E

D

-

W

-

A

-

R

-

T

P

G

P

R

R

P

D

Q

R

A

A

I

-

P

-

A

-

E

-

L

L

T

V

D

D

R

H

L

-

D

E

T

N

T

V

F

I

L

S

T

C

P

P

H
|
H

L

L

G
|
G

S

A

A

S

V

T

E

K

S

E

V

A

R

Q

K

S

N

R

I

C

A

G

M

E

E

E

A

I

A

A

V

V

N

Q

I

F

V

V

E

D

binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ L101), A100 (≠ T105), R149 (≠ E156), I150 (≠ V157), Y168 (= Y175), D169 (= D176), P170 (≠ T177), I171 (≠ C178), H200 (≠ A208), T201 (≠ L209), P202 (= P210), T207 (= T215), C228 (≠ V236), A229 (≠ G237), R230 (= R238), H277 (= H294), G279 (= G296)

7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
30% identity, 95% coverage: 5:316/330 of query aligns to 3:300/302 of 7ewhA

query
sites
7ewhA

K

K

I

V

L

L

I

I

T

S

H

D

W

S

V

L

H

D

E

P

E

C

I

C

I

R

E

K

Y

I

L

L

S

Q

E

D

Y

G

G

G

E

L

V

Q

V

V

T

V

N

E

Q

-

T

-

R

K

Q

Q

S

N

M

L

K

S