SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035702077.1 NCBI__GCF_000691145.1:WP_035702077.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

P54582 Glycine betaine transporter BetP; Glycine betaine permease from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see 6 papers)
43% identity, 99% coverage: 5:509/510 of query aligns to 56:571/595 of P54582

query
sites
P54582

S

A

S

S

V

L

F

N

W

W

I

S

V

V

I

I

A

V

-

P

-

A

-

L

-

V

I

I

T

V

F

L

I

A

A

T

V

V

L

V

W

W

G

G

A

I

L

G

S

F

P

K

D

D

T

S

L

F

Q

T

N

N

V

F

T

A

D

S

Q

S

I

A

Q

L

T

S

Y

A

I

V

T

V

N

D

D

N

F

L

G

G

W
|
W

Y

A

Y

F

L

I

L

L

V

F

V

G

S

T

L

V

L

F

V

V

G

F

F

F

C

I

L

V

F

V

F

I

I

A

F

A

S

S

P

K

I

F

G

G

K

T

I

I

T

R

L

L

G

G

K

R

P

I

G

D

E

E

E

A

P

P

E
|
E

F

F

G

R

L

T

F

V

S

S

W

W

F

I

A

S

M

M

L

M

F

F

S

A

A

A

G
|
G

M
|
M

G
|
G

I
|
I

G
|
G

L

L

V

M

F
|
F

Y

Y

G

G

A

T

E

E

P

P

I

L

S

T

H

F

F

Y

A

R

I

N

Q

G

S

V

P

P

T

G

G

H

E

D

T

E

E

H

N

N

A

V

Q

G

A

V

F

-

R

-

D

-

S

A

L

M

R

S

Y

T

T

T

F

M

F

F

H

H

W
|
W

G

T

L

L

H

H

A

P

W
|
W

A

A

I

I

Y
|
Y

A

A

I

I

V

V

A

G

L

L

C

A

I

I

A

A

Y

Y

F

S

K

T

F

F

R
|
R

K

V

D

G

A

R

P

K

G

Q

L

L

I

L

S

S

T

A

L

F

Y

V

P

P

V

L

F

I

G

G

N

E

R

K

-

G

I

A

H

E

G

G

W

W

I

L

G

G

K

K

T

L

I

I

D

D

C

I

I

L

A

A

V

I

F

I

A

A

T

T

V

V

V

F

G

G

V

T

A

A

T

C

S
|
S

L

L

G

G

L

L

G

G

A

A

A

L

Q

Q

I

I

N

G

G

A

G

G

L

L

N

S

Y

A

-

A

-

N

L

I

F

I

G

E

I

D

P

P

N

S

N

D

F

W

L

-

T

T

Q

I

L

V

I

G

I

I

I

V

A

S

I

V

V

L

T

T

V

L

L

A

F

F

L

I

L

F

S

S

A

A

W

I

S

S

G

G

I

V

G

G

K

K

G
|
G

I

I

K

Q

Y

Y

L

L

S

S

N

N

S

A

N
|
N

M

M

I

V

F

L

A

A

L

L

M

A

I

I

F

F

L

V

L

F

I

V

V

V

G

G

P

P

T

T

V

V

Y

S

I

I

L

L

N

N

S

L

F

L

T

P

D

G

S

S

I

I

G

G

Q

N

Y

Y

L

L

S

S

N

N

I

F

V

F

Q

Q

M

M

S

A

F

G

R

R

L
x
T

S

A

P

M

N

S

-

A

D

D

P

G

E

T

K

A

R

G

E

E

W
|
W

I

L

N

G

G

S

W
|
W

T

T

I

I

F
|
F

Y

Y

W
|
W

A

A

W
|
W

I
|
I

S
|
S

W
|
W

S

S

P

P

F
|
F

V

V

G

G

I

M

F
|
F

I

L

A

A

R
|
R

V

I

S

S

R

R

G

G

R
|
R

T

S

I

I

R

R

E

E

F

F

L

I

I

L

G

G

V

V

L

L

V

L

A

V

P

P

S

A

I

G

L

V

V

S

F

T

L

V

W

W

F

F

S

S

I

I

F

F

G

G

V

G

S

T

A

A

M

I

D

V

L

F

Q

E

Q

Q

N

N

N

G

I

E

V

S

D

I

V

W

A

G

N

D

M

G

S

A

T

A

E

E

T

E

M

Q

L

L

F

F

G

G

V

L

L

L

N

H

E

A

Y

L

P

P

L

G

A

G

M

Q

I

I

T

M

S

G

I

I

L

I

A

A

L

M

I

I

L

L

I

L

A

G

V

T

F

F

I

I

T

T

S

S

A

A

D

D

S

S

A

A

T

S

F

T

V

V

L

M

G

G

M

T

Q

M

T

S

T

Q

Y

H

G

G

S

Q

L

L

N

E

P

A

A

N

N

K

S

W

V

V

K

T

I

A

S

A

W

W

G

G

I

V

I

A

Q

T

S

A

A

A

V

I

A

G

A

L

V

T

L

L

F

L

S

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

N

N

T

V

A

T

K

I

L

V

A

A

L

T

P

P

F

F

S

L

V

F

V

V

I

V

I

I

L

G

M

L

I

M

V

F

S

A

L

L

Y

V

K

K

S

D

L

L

N

S

E

N

E

D

-

V

-

I

R

Y

R

L

A

E

I

Y

K

R

Q

E

A

Q

N

Q

K

R

Y

F

N

N

K

A

-

R

-

L

P

A

R

R

S

E

P

R

R

R

V

V

K

H

K

N

W101 (= W46) mutation to A: Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-351.
E135 (= E80) mutation to A: Strongly decreased betaine transport.
G149 (= G94) mutation to A: Decreases betaine transport. No effect on activation by increased osmolality.
M150 (= M95) mutation to F: No effect on activation by increased osmolality; when associated with A-152.
G151 (= G96) mutation to A: Nearly abolishes betaine transport.
I152 (= I97) mutation to A: No effect on activation by increased osmolality; when associated with F-150.
IG 152:153 (= IG 97:98) binding glycine betaine
G153 (= G98) mutation to A: Decreases betaine transport and alters activation at higher osmolality.; mutation to D: Changes substrate specificity, giving rise to proton-coupled choline transport. Decreases sodium-dependent betaine transport.
F156 (= F101) mutation to A: Decreases betaine transport, but has no major effect on affinity for glycine betaine.
W189 (= W137) mutation to C: Mildly decreased betaine transport.
W194 (= W142) mutation to L: Strongly decreased betaine transport.
Y197 (= Y145) mutation to L: Nearly abolishes betaine transport.
R210 (= R158) mutation to A: Nearly abolishes betaine transport.
S253 (= S200) binding glycine betaine
G301 (= G247) mutation to L: Strongly decreased betaine transport.
N309 (= N255) mutation to A: Decreases affinity for sodium ions.
T351 (≠ L297) mutation to A: Mainly trimeric, but shows reduced activity at high osmolalities. Mainly monomeric, shows a decrease in activity and cannot be activated in response to increased osmolality; when associated with A-101.
W362 (= W307) mutation to C: Strongly decreased betaine transport.
W366 (= W311) mutation to C: No effect on betaine transport.
F369 (= F314) mutation to G: Decreases affinity for glycine betaine. Decreases betaine transport.
W371 (= W316) mutation to L: No effect on betaine transport.
W373 (= W318) mutation to A: Strongly decreases affinity for glycine betaine and betaine transport.
WWISW 373:377 (= WWISW 318:322) binding glycine betaine
W374 (= W319) mutation to A: Strongly decreases betaine transport, but has no major effect on affinity for glycine betaine.; mutation to L: No effect on betaine transport.
W377 (= W322) mutation to A: Abolishes betaine transport.; mutation to L: Nearly abolishes betaine transport.
F380 (= F325) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
F384 (= F329) mutation to A: Decreases betaine transport, but has no effect on affinity for glycine betaine.
R387 (= R332) mutation to A: Mildly decreased betaine transport.
R392 (= R337) mutation to K: Moderately decreased betaine transport.

4llhA Substrate bound outward-open state of the symporter betp (see paper)
43% identity, 98% coverage: 12:509/510 of query aligns to 11:512/524 of 4llhA

query
sites
4llhA

I

L

A

V

I

I

T

V

F

L

I

A

A

T

V

V

L

V

W

W

G

G

A

I

L

G

S

F

P

K

D

D

T

S

L

F

Q

T

N

N

V

F

T

A

D

S

Q

S

I

A

Q

L

T

S

Y

A

I

V

T

V

N

D

D

N

F

L

G

G

W

W

Y

A

Y

F

L

I

L

L

V

F

V

G

S

T

L

V

L

F

V

V

G

F

F

F

C

I

L

V

F

V

F

I

I

A

F

A

S

S

P

K

I

F

G

G

K

T

I

I

T

R

L

L

G

G

K

R

P

I

G

D

E

E

E

A

P

P

E

E

F

F

G

R

L

T

F

V

S

S

W

W

F

I

A

S

M

M

L

M

F

F

S
x
A

A

A

G

G

M
|
M

G
|
G

I

I

G
x
D

L

L

V

M

F

F

Y

Y

G

G

A

T

E

E

P

P

I

L

S

T

H

F

F

Y

A

R

I

N

Q

G

S

V

P

P

T

G

G

H

E

D

T

E

E

H

N

N

A

V

Q

G

A

V

F

-

R

-

D

-

S

A

L

M

R

S

Y

T

T

T

F

M

F

F

H

H

W

W

G

T

L

L

H

H

A

P

W

W

A

A

I

I

Y

Y

A

A

I

I

V

V

A

G

L

L

C

A

I

I

A

A

Y

Y

F

S

K

T

F

F

R

R

K

V

D

G

A

R

P

K

G

Q

L

L

I

L

S

S

T

A

L

F

Y

V

P

P

V

L

F

I

G

G

N

E

R

K

-

G

I

A

H

E

G

G

W

W

I

L

G

G

K

K

T

L

I

I

D

D

C

I

I

L

A

A

V

I

F

I

A

A

T

T

V

V

V

F

G

G

V

T

A

A

T

C

S

S

L

L

G

G

L

L

G

G

A

A

A

L

Q

Q

I

I

N

G

G

A

G

G

L

L

N

S

Y

A

L

A

F

E

G

D

I

-

P

P

N

S

N

D

F

W

L

-

T

T

Q

I

L

V

I

G

I

I

I

V

A

S

I

V

V

L

T

T

V

L

L

A

F

F

L

I

L

F

S

S

A

A

W

I

S

S

G

G

I

V

G

G

K

K

G

G

I

I

K

Q

Y

Y

L

L

S

S

N

N

S

A

N

N

M

M

I

V

F

L

A

A

L

L

M

A

I

I

F

F

L

V

L

F

I

V

V

V

G

G

P

P

T

T

V

V

Y

S

I

I

L

L

N

N

S

L

F

L

T

P

D

G

S

S

I

I

G

G

Q

N

Y

Y

L

L

S

S

N

N

I

F

V

F

Q

Q

M

M

S

A

F

G

R

R

L

T

S

A

P

M

N

S

-

A

D

D

P

G

E

T

K

A

R

G

E

E

W

W

I

L

N

G

G

S

W

W

T

T

I

I

F

F

Y

Y

W

W

A

A

W
|
W

I

I

S

S

W
|
W

S

S

P

P

F

F

V

V

G

G

I

M

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

S

I

I

R

R

E

E

F

F

L

I

I

L

G

G

V

V

L

L

V

L

A

V

P

P

S

A

I

G

L

V

V

S

F

T

L

V

W

W

F

F

S

S

I

I

F

F

G

G

V

G

S

T

A

A

M

I

D

V

L

F

Q

E

Q

Q

N

N

N

G

I

E

V

S

D

I

V

W

A

G

N

D

M

G

S

A

T

A

E

E

T

E

M

Q

L

L

F

F

G

G

V

L

L

L

N

H

E

A

Y

L

P

P

L

G

A

G

M

Q

I

I

T

M

S

G

I

I

L

I

A

A

L

M

I

I

L

L

I

L

A

G

V

T

F
|
F

F

F

I

I

T
|
T

S
|
S

A

A

D

D

S

S

A

A

T

S

F

T

V

V

L

M

G

G

M

T

Q

M

T

S

T

Q

Y

H

G

G

S

Q

L

L

N

E

P

A

A

N

N

K

S

W

V

V

K

T

I

A

S

A

W

W

G

G

I

V

I

A

Q

T

S

A

A

A

V

I

A

G

A

L

V

T

L

L

F

L

S

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

N

N

T

V

A

T

K

I

L

V

A

A

L

T

P

P

F

F

S

L

V

F

V

V

I

V

I

I

L

G

M

L

I

M

V

F

S

A

L

L

Y

V

K

K

S

D

L

L

N

S

E

N

E

D

-

V

-

I

R

Y

R

L

A

E

I

Y

K
x
R

Q

E

A

Q

N

Q

K
x
R

Y

F

N

N

K

A

-

R

-

L

P

A

R

R

S

E

P

R

R

R

V

V

K

H

K

N

binding 2-(trimethyl-lambda~5~-arsanyl)ethanol: M94 (= M95), G95 (= G96), D97 (≠ G98), W314 (= W318), W315 (= W319), W318 (= W322)
binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: R495 (≠ K494), R499 (≠ K498)
binding sodium ion: A91 (≠ S92), M94 (= M95), G95 (= G96), F405 (= F409), T408 (= T412), S409 (= S413)

3p03C Crystal structure of betp-g153d with choline bound (see paper)
43% identity, 98% coverage: 12:509/510 of query aligns to 11:508/508 of 3p03C

query
sites
3p03C

I

L

A

V

I

I

T

V

F

L

I

A

A

T

V

V

L

V

W

W

G

G

A

I

L

G

S

F

P

K

D

D

T

S

L

F

Q

T

N

N

V

F

T

A

D

S

Q

S

I

A

Q

L

T

S

Y

A

I

V

T

V

N

D

D

N

F

L

G

G

W

W

Y

A

Y

F

L

I

L

L

V

F

V

G

S

T

L

V

L

F

V

V

G

F

F

F

C

I

L

V

F

V

F

I

I

A

F

A

S

S

P

K

I

F

G

G

K

T

I

I

T

R

L

L

G

G

K

R

P

I

G

D

E

E

E

A

P

P

E

E

F

F

G

R

L

T

F

V

S

S

W

W

F

I

A

S

M

M

L

M

F

F

S

A

A
|
A

G

G

M
|
M

G

G

I

I

G
x
D

L

L

V

M

F

F

Y

Y

G

G

A

T

E

E

P

P

I

L

S

T

H

F

F

Y

A

R

I

N

Q

G

S

V

P

P

T

G

G

H

E

D

T

E

E

H

N

N

A

V

Q

G

A

V

F

-

R

-

D

-

S

A

L

M

R

S

Y

T

T

T

F

M

F

F

H

H

W

W

G

T

L

L

H

H

A

P

W

W

A

A

I

I

Y

Y

A

A

I

I

V

V

A

G

L

L

C

A

I

I

A

A

Y

Y

F

S

K

T

F

F

R

R

K

V

D

G

A

R

P

K

G

Q

L

L

I

L

S

S

T

A

L

F

Y

V

P

P

V

L

F

I

G

G

N

E

R

K

-

G

I

A

H

E

G

G

W

W

I

L

G

G

K

K

T

L

I

I

D

D

C

I

I

L

A

A

V

I

F

I

A

A

T

T

V

V

V

F

G

G

V

T

A

A

T

C

S

S

L

L

G

G

L

L

G

G

A

A

A

L

Q

Q

I

I

N

G

G

A

G

G

L

L

N

S

Y

-

L

-

F

-

G

-

I

-

P

A

N

A

N

N

F

I

L

I

T

D

Q

W

L

T

I

I

-

V

-

G

I

I

I

V

A

S

I

V

V

L

T

T

V

L

L

A

F

F

L

I

L

F

S

S

A

A

W

I

S

S

G

G

I

V

G

G

K

K

G

G

I

I

K

Q

Y

Y

L

L

S

S

N

N

S

A

N

N

M

M

I

V

F

L

A

A

L

L

M

A

I

I

F

F

L

V

L

F

I

V

V

V

G

G

P

P

T

T

V

V

Y

S

I

I

L

L

N

N

S

L

F

L

T

P

D

G

S

S

I

I

G

G

Q

N

Y

Y

L

L

S

S

N

N

I

F

V

F

Q

Q

M

M

S

A

F

G

R

R

L

T

S

A

P

M

N

S

-

A

D

D

P

G

E

T

K

A

R

G

E

E

W

W

I

L

N

G

G

S

W

W

T

T

I

I

F

F

Y

Y

W

W

A

A

W

W

I

I

S

S

W

W

S

S

P

P

F
|
F

V

V

G

G

I

M

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

S

I

I

R

R

E

E

F

F

L

I

I

L

G

G

V

V

L

L

V

L

A

V

P

P

S

A

I

G

L

V

V

S

F

T

L

V

W

W

F

F

S

S

I

I

F

F

G

G

V

G

S

T

A

A

M

I

D

V

L

F

Q

E

Q

Q

N

N

N

G

I

E

V

S

D

I

V

W

A

G

N

D

M

G

S

A

T

A

E

E

T

E

M

Q

L

L

F

F

G

G

V

L

L

L

N

H

E

A

Y

L

P

P

L

G

A

G

M

Q

I

I

T

M

S

G

I

I

L

I

A

A

L

M

I

I

L

L

I

L

A

G

V

T

F

F

I

I

T

T

S

S

A

A

D

D

S

S

A

A

T

S

F

T

V

V

L

M

G

G

M

T

Q

M

T

S

T

Q

Y

H

G

G

S

Q

L

L

N

E

P

A

A

N

N

K

S

W

V

V

K

T

I

A

S

A

W

W

G

G

I

V

I

A

Q

T

S

A

A

A

V

I

A

G

A

L

V

T

L

L

F

L

S

S

G

G

-

D

-

N

-

A

L

L

Q

S

A

N

L

L

Q

Q

N

N

T

V

A

T

K

I

L

V

A

A

L

T

P

P

F

F

S

L

V

F

V

V

I

V

I

I

L

G

M

L

I

M

V

F

S

A

L

L

Y

V

K

K

S

D

L

L

N

S

E

N

E

D

-

V

-

I

R

Y

R

L

A

E

I

Y

K

R

Q

E

A

Q

N

Q

K

R

Y

F

N

N

K

A

P

-

R

R

S

L

P

A

R

R

V

E

K

R

binding choline ion: A92 (= A93), M94 (= M95), D97 (≠ G98), F320 (= F325)

8ybqA Choline transporter bett - cht bound (see paper)
40% identity, 90% coverage: 37:493/510 of query aligns to 39:501/502 of 8ybqA

query
sites
8ybqA

Q

Q

T

N

Y

W

I

A

T

A

N

N

D

T

F

V

G

G

W

W

Y

Y

L

M

V

V

V

M

S

T

L

L

L

Y

V

L

G

V

F

F

C

V

L

V

F

V

F

T

I

A

F

L

S

S

P

G

I

F

G

G

K

K

I

I

T

K

L

L

G

G

K

A

P

D

G

H

E

D

E

E

P

P

E

E

F

F

G

S

L

Y

F

L

S

S

W

W

F

A

A

G

M

M

L

L

F

F

S

A

A

A

G
|
G

M

I

G

S

I

I

G

T

L

L

V

F

F

F

Y

F

G

C

A

V

A

S

E

E

P

P

I

L

S

T

H

H

F

L

A

-

I

L

Q

Q

S

P

P

P

T

Q

G

G

E

E

T

G

E

G

N

T

A

A

Q

E

A

A

F

A

R

R

D

Q

S

G

L

M

R

Q

Y

L

T

L

F

F

F

L

H

H

W

W

G

G

L

L

H

H

A

G

W

W

A

G

I

V

Y

F

A

A

I

F

V

V

A

G

L

M

C

A

I

L

A

A

Y

Y

F

F

K

A

F

Y

R

R

K

H

D

N

A

L

P

P

G

L

L

A

I

L

S

R

S

S

T

A

L

L

Y

Y

P

P

V

L

F

I

G

G

N

K

R

R

I

I

H

N

G

G

W

P

I

I

G

G

K

Y

T

A

I

V

D

D

C

G

I

F

A

G

V

I

F

I

A

A

T

T

V

I

V

F

G

G

V

L

A

G

T

A

S

D

L

M

G

G

L

F

G

G

A

V

A

L

Q

H

I

L

N

N

G

S

G

G

L

L

N

D

Y

Y

L

L

F

F

G

G

I

V

P

P

N

H

N

T

F

Q

L

W

T

I

Q

Q

L

V

I

G

I

L

I

I

A

T

I

L

V

M

T

M

V

G

L

A

F

A

L

I

L

L

S

V

A

A

W

I

S

A

G

G

I

V

G

D

K

K

G

G

I

V

K

R

Y

V

L

M

S

S

N

D

S

I

N

N

M

M

I

L

F

L

A

A

A

C

L

A

L

L

M

L

I

L

F

F

L

V

L

L

I

F

V

A

G

G

P

P

T

T

V

Q

Y

H

I

L

L

L

N

N

S

T

F

L

T

V

D

Q

S

N

I

I

G

G

Q

D

Y

Y

L

L

S

G

N

A

I

L

V

P

Q

S

M

K

S

S

F

F

R

D

L

V

-

Y

S

A

P

Y

N

N

D

K

P

P

E

S

K

-

R

-

E

D

W

W

I

L

N

G

G

G

W

W

T

T

I

V

F

F

Y

Y

W

W

A

A

W
|
W

I

I

S

A

W

W

S

A

P

P

F

F

V

V

G

G

I

L

F

F

I

I

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

I

R

R

E

E

F

F

L

V

I

F

G

G

V

V

L

L

V

L

A

I

P

P

S

L

I

G

L

F

V

T

F

L

L

A

W

W

F

M

S

S

I

I

F

F

G

G

V

N

S

S

A

A

M

I

D

D

L

Q

Q

V

Q

L

N

N

N

H

I

G

V

M

D

A

V

A

A

L

N

G

M

Q

S

S

T

A

-

I

-

D

-

P

E

S

T

M

M

T

L

L

F

Y

G

L

V

L

L

L

N

E

E

T

Y

Y

P

P

L

W

A

S

M

K

I

T

T

V

S

I

I

A

L

V

A

T

L

V

I

F

L

I

I

S

A

F

V

V

F

F

I

V

T

T

S

S

A

A

D

D

S

S

A

G

T

T

F

V

V

V

L

L

G

S

M

T

Q

L

T

S

T

A

Y

K

G

G

S

G

L

-

N

N

P

P

A

D

N

E

S

D

-

G

-

P

-

K

-

W

V

L

K

R

I

V

S

F

W

W

G

G

I

V

I

A

Q

T

S

A

A

L

V

I

A

T

A

S

V

G

L

L

F

F

S

S

G

G

G

S

L

I

Q

D

A

A

L

L

Q

K

N

S

T

A

A

V

K

V

L

L

A

T

A

S

L

L

P

P

F

F

S

S

V

L

V

I

I

L

L

L

M

M

I

M

V

W

S

G

L

L

Y

H

K

K

S

A

-

F

L

V

N

M

E

E

E

S

R

Q

R

R

A

Q

I

I

binding choline ion: G96 (= G94), W318 (= W318), W319 (= W319)

B4EY22 L-carnitine/gamma-butyrobetaine antiporter from Proteus mirabilis (strain HI4320) (see 2 papers)
30% identity, 95% coverage: 27:509/510 of query aligns to 34:511/514 of B4EY22

query
sites
B4EY22

D

D

T

A

L

S

Q

N

N

E

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

Y

A

Y

F

L

E

L

W

V

Y

V

M

S

V

L

I

L

M

V

F

G

G

F

G

C

W

L

F

F

W

F

L

I

V

F

F

S

G

P

R

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

D

P

-

G

-

E

E

E

K

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E
|
E

P

-

I

I

S

Y

H

Y

F

Y

A

I

I

S

Q

S

S

P

P

P

T

F

G

G

-

M

E

E

T

G

E

Y

N

S

A

A

Q

P

A

A

F

K

R

E

D

I

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W

W

G

G

L

P

H

L

A

P

W

W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

T

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

V

H

N

G

G

W

L

I

F

G

G

K

T

T

V

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

I

N

Q

Y

Y

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

S

I

C

V

W

T

I

V

L

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

F

G

G

I

L

G

Q

K

K

G

G

I

V

K

K

Y

I

L

A

S

S

N

D

S

V

N
x
R

M

T

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

G

P

G

T

A

V

S

Y

F

I

I

L

V

N

N

S

Y

F

F

T

T

D

D

S

S

I

V

G

G

Q

T

Y

L

L

L

S

M

N

Y

I

M

V

P

Q

R

M

M

S

L

F

F

R

Y

L

T

S

D

P

P

N

I

D

G

P

-

E

-

K

K

R

G

E

G

W

F

I

P

N

Q

G

A

W
|
W

T

T

I

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V
x
M

G

S

I

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

L

G

G

V

M

L

V

V

S

A

G

P

L

S

T

I

A

L

G

V

T

F

W

L

L

W

I

F

W

S

T

I

I

F

L

G

G

V

G

S

N

A

T

M

L

D

Q

L

L

Q

I

Q

D

N

Q

N

N

I

I

V

L

D

N

V

I

A

P

N

Q

M

L

S

I

T

D

E

Q

T

Y

M

G

L

V

F

P

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

I

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

Y

R

G

S

-

M

-

K

-

E

S

G

L

A

N

E

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

V

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

I

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

-

K

-

S

-

L

-

N

-

E

-

R

-

A

-

I

I

K

K

Q

D

A

A

N

K

K

V

Y

H

N

W

K

K

P

D

R

C

S

S

P

P

R

Y

V

T

K

Q

K

K

E111 (= E106) mutation to A: Abolishes transport activity.
R262 (≠ N255) mutation R->A,E: Strong decrease in L-carnitine transport. Mutant is Na(+)-dependent for substrate binding and transport.
W316 (= W311) mutation to A: 2.5-fold decrease in Vmax.
M331 (≠ V326) mutation to V: 10-fold decrease in Vmax.

P31553 L-carnitine/gamma-butyrobetaine antiporter from Escherichia coli (strain K12) (see 3 papers)
30% identity, 91% coverage: 27:491/510 of query aligns to 34:503/504 of P31553

query
sites
P31553

D

D

T

A

L

A

Q

N

N

V

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

-

A

Y

F

Y

E

L

W

L

Y

V

M

V

V

S

V

L

M

L

L

V

F

G

G

F

W

C

-

L

F

F

W

F

L

I

V

F

F

S

G

P

P

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

N

P

-

G

-

E

E

E

P

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E

E

P

-

I

I

S

Y

H
x
Y

F

Y

A

I

I

S

Q

T

S

P

P

P

T

F

G

G

E

L

T

E

E

P

N

N

A

S

Q

T

A

G

F

A

R

K

D

E

-

L

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

L

P

H

L

A

P

W

W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

V

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

A

H

K

G

G

W

L

I

F

G

G

K

T

T

I

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

M

N

Q

Y

W

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

T

I

C

V

W

T

I

V

I

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

C

G

G

I

L

G

Q

K

K

G

G

I

V

K

R

Y

I

L

A

S

S

N

D

S

V

N

R

M

S

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

S

P

G

T

A

V

S

Y

F

I

I

L

M

N

N

S

Y

F

F

T

T

D
|
D

S

S

I

V

G

G

Q

M

Y

L

L

L

S
x
M

N

Y

I

L

V

P

Q
x
R

M

M

S

L

F

F

R

Y

L
x
T

S

D

P

P

N

I

D

-

P

-

E

A

K

K

R

G

E

G

W

F

I

P

N

Q

G
|
G

W
|
W

T

T

I

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S
x
A

P
x
I

F
x
Q

V
x
M

G

S

I

I

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

F

G

G

V

M

L

V

V

L

A

G

P

L

S

T

I

A

L

S

V

T

F

W

L

I

W

L

F

W

S

T

I

V

F

L

G

G

V

S

S

N

A

T

M

L

D

L

L

L

Q

I

Q

D

N

K

N

N

I

I

V

I

D

N

V

I

A

P

N

N

M

L

S

I

T

E

E

Q

T

Y

M

G

L

V

F

A

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

V

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

-

R

-

E

-

V

Y

R

G

D

S

G

L

E

N

E

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

I

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

V

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

I

K

K

S

D

L

A

N

K

E

Q

E

N

R

W

R

K

Y114 (≠ H110) binding 4-(trimethylamino)butanoate; mutation to L: Small decrease in transport activity.
W142 (= W137) binding (R)-carnitine
D288 (= D281) mutation to A: Retains 70% of transport activity. Forms mostly monomers.; mutation to R: Abolishes transport activity. Forms mostly monomers.; mutation to W: Retains 4% of transport activity. Forms mostly monomers.
M295 (≠ S288) mutation to E: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers.
R299 (≠ Q292) mutation to A: Does not affect transport activity. Forms mostly monomers. Can also form small amounts of homodimers and homotrimers. Shows a high tendency to aggregate.
T304 (≠ L297) mutation to A: Does not affect transport activity. Forms mostly monomers. Shows a high tendency to aggregate.
GW 315:316 (= GW 310:311) binding 4-(trimethylamino)butanoate
W316 (= W311) mutation to L: Decrease in transport activity.
W323 (= W318) binding 4-(trimethylamino)butanoate; mutation to L: Abolishes transport activity.
WW 323:324 (= WW 318:319) binding (R)-carnitine
W324 (= W319) mutation to L: Abolishes transport activity.
Y327 (≠ W322) mutation to L: Strong decrease in transport activity.
YAIQ 327:330 (≠ WSPF 322:325) binding (R)-carnitine
Q330 (≠ F325) mutation to L: Decrease in transport activity.
M331 (≠ V326) binding 4-(trimethylamino)butanoate

3hfxA Crystal structure of carnitine transporter (see paper)
30% identity, 91% coverage: 27:491/510 of query aligns to 23:492/493 of 3hfxA

query
sites
3hfxA

D

D

T

A

L

A

Q

N

N

V

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

-

A

Y

F

Y

E

L

W

L

Y

V

M

V

V

S

V

L

M

L

L

V

F

G

G

F

W

C

-

L

F

F

W

F

L

I

V

F

F

S

G

P

P

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

N

P

-

G

-

E

E

E

P

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E

E

P

-

I

I

S

Y

H
x
Y

F

Y

A

I

I

S

Q

T

S

P

P

P

T

F

G

G

E

L

T

E

E

P

N

N

A

S

Q

T

A

G

F

A

R

K

D

E

-

L

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

L

P

H

L

A

P

W

W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

V

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

A

H

K

G

G

W

L

I

F

G

G

K

T

T

I

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

M

N

Q

Y

W

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

T

I

C

V

W

T

I

V

I

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

C

G

G

I

L

G

Q

K

K

G

G

I

V

K

R

Y

I

L

A

S

S

N

D

S

V

N

R

M

S

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

S

P

G

T

A

V

S

Y

F

I

I

L

M

N

N

S

Y

F

F

T

T

D

D

S

S

I

V

G

G

Q

M

Y

L

L

L

S

M

N

Y

I

L

V

P

Q

R

M

M

S

L

F

F

R

Y

L

T

S

D

P

P

N

I

D

-

P

-

E

A

K

K

R

G

E

G

W

F

I

P

N

Q

G

G

W
|
W

T

T

I

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W
x
Y

S

A

P

I

F
x
Q

V

M

G

S

I

I

F
|
F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

F

G

G

V

M

L

V

V

L

A

G

P

L

S

T

I

A

L

S

V

T

F

W

L

I

W

L

F

W

S

T

I

V

F

L

G

G

V

S

S

N

A

T

M

L

D

L

L

L

Q

I

Q

D

N

K

N

N

I

I

V

I

D

N

V

I

A

P

N

N

M

L

S

I

T

E

E

Q

T

Y

M

G

L

V

F

A

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

V

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

-

R

-

E

-

V

Y

R

G

D

S

G

L

E

N

E

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

I

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

V

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

I

K

K

S

D

L

A

N

K

E

Q

E

N

R

W

R

K

binding carnitine: Y103 (≠ H110), W131 (= W137), W305 (= W311), W312 (= W318), W313 (= W319), Y316 (≠ W322), Y316 (≠ W322), Q319 (≠ F325), F323 (= F329)

2wswA Crystal structure of carnitine transporter from proteus mirabilis (see paper)
30% identity, 90% coverage: 27:485/510 of query aligns to 39:502/508 of 2wswA

query
sites
2wswA

D

D

T

A

L

S

Q

N

N

E

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

Y

A

Y

F

L

E

L

W

V

Y

V

M

S

V

L

I

L

M

V

F

G

G

F

G

C

W

L
x
F

F

W

F

L

I

V

F
|
F

S

G

P
x
R

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

D

P

-

G

-

E

E

E

K

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E

E

P

-

I

I

S

Y

H

Y

F

Y

A

I

I

S

Q

S

S

P

P

P

T

F

G

G

-

M

E

E

T

G

E

Y

N

S

A

A

Q

P

A

A

F

K

R

E

D

I

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W

W

G

G

L

P

H

L

A

P

W

W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

V

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

V

H

N

G

G

W

L

I

F

G

G

K

T

T

V

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

I

N

Q

Y

Y

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

S

I

C

V

W

T

I

V

L

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

F

G

G

I

L

G

Q

K

K

G

G

I

V

K

K

Y

I

L

A

S

S

N

D

S

V

N

R

M

T

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

G

P

G

T

A

V

S

Y

F

I

I

L

V

N

N

S

Y

F

F

T

T

D

D

S

S

I

V

G

G

Q

T

Y

L

L

L

S

M

N

Y

I

M

V

P

Q

R

M

M

S

L

F

F

R

Y

L

T

S

D

P

P

N

I

D

G

P

-

E

-

K

K

R

G

E

G

W

F

I

P

N

Q

G

A

W

W

T

T

I

V

F

F

Y

Y

W

W

A

A

W

W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

I

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

L

G

G

V

M

L

V

V

S

A

G

P

L

S

T

I

A

L

G

V

T

F

W

L

L

W

I

F

W

S

T

I

I

F

L

G

G

V

G

S

N

A

T

M

L

D

Q

L

L

Q

I

Q

D

N

Q

N

N

I

I

V

L

D

N

V

I

A

P

N

Q

M

L

S

I

T

D

E

Q

T

Y

M

G

L

V

F

P

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

I

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

Y

R

G

S

-

M

-

K

-

E

S

G

L

A

N

D

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

V

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

I

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

I

K

K

S

D

binding 5-cyclohexyl-1-pentyl-beta-d-maltoside: F72 (≠ L60), F76 (= F64), R78 (≠ P66)

4m8jA Crystal structure of cait r262e bound to gamma-butyrobetaine (see paper)
30% identity, 90% coverage: 27:485/510 of query aligns to 26:489/495 of 4m8jA

query
sites
4m8jA

D

D

T

A

L

S

Q

N

N

E

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

Y

A

Y

F

L

E

L

W

V

Y

V

M

S

V

L

I

L

M

V

F

G

G

F

G

C

W

L

F

F

W

F

L

I

V

F

F

S

G

P

R

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

D

P

-

G

-

E

E

E

K

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E

E

P

-

I

I

S

Y

H

Y

F

Y

A

I

I

S

Q

S

S

P

P

P

T

F

G

G

-

M

E

E

T

G

E

Y

N

S

A

A

Q

P

A

A

F

K

R

E

D

I

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W

W

G

G

L

P

H

L

A

P

W
|
W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

V

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

V

H

N

G

G

W

L

I

F

G

G

K

T

T

V

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

L

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

I

N

Q

Y

Y

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

S

I

C

V

W

T

I

V

L

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

F

G

G

I

L

G

Q

K

K

G

G

I

V

K

K

Y

I

L

A

S

S

N

D

S

V

N

E

M

T

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

G

P

G

T

A

V

S

Y

F

I

I

L

V

N

N

S

Y

F

F

T

T

D

D

S

S

I

V

G

G

Q

T

Y

L

L

L

S

M

N

Y

I

M

V

P

Q

R

M

M

S

L

F

F

R

Y

L

T

S

D

P

P

N

I

D

G

P

-

E

-

K

K

R

G

E

G

W

F

I

P

N

Q

G

A

W

W

T

T

I

V

F

F

Y

Y

W

W

A

A

W
|
W

I

V

S

I

W

Y

S

A

P

I

F

Q

V

M

G

S

I

I

F

F

I

L

A

A

R

R

V

I

S

S

R

K

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

L

G

G

V

M

L

V

V

S

A

G

P

L

S

T

I

A

L

G

V

T

F

W

L

L

W

I

F

W

S

T

I

I

F

L

G

G

V

G

S

N

A

T

M

L

D

Q

L

L

Q

I

Q

D

N

Q

N

N

I

I

V

L

D

N

V

I

A

P

N

Q

M

L

S

I

T

D

E

Q

T

Y

M

G

L

V

F

P

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

I

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

Y

R

G

S

-

M

-

K

-

E

S

G

L

A

N

D

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

V

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

I

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q

Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

I

K

K

S

D

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W139 (= W142), W315 (= W318), W316 (= W319)

2wsxA Crystal structure of carnitine transporter from escherichia coli (see paper)
29% identity, 91% coverage: 27:491/510 of query aligns to 27:496/496 of 2wsxA

query
sites
2wsxA

D

D

T

A

L

A

Q

N

N

V

V

V

T

I

D

N

Q

A

I

V

Q

F

T

S

Y

Y

I

V

T

T

N

N

D

V

F

W

G

G

W

W

-

A

Y

F

Y

E

L

W

L

Y

V

M

V

V

S

V

L

M

L

L

V

F

G

G

F

W

C

-

L

F

F

W

F

L

I

V

F

F

S

G

P

P

I

Y

G

A

K

K

I

K

T

R

L

L

G

G

K

E

P

-

G

-

E

E

E

P

P

P

E

E

F

F

G

S

L

T

F

A

S

S

W

W

F

I

A

F

M

M

L

M

F

F

S

A

A

S

G

C

M

T

G

S

I

A

G

A

L

V

V

L

F

F

Y

W

G

G

A

S

A

I

E

E

P

-

I

I

S

Y

H

Y

F

Y

A

I

I

S

Q

T

S

P

P

P

T

F

G

G

E

L

T

E

E

P

N

N

A

S

Q

T

A

G

F

A

R

K

D

E

-

L

S

G

L

L

R

A

Y

Y

T

S

F

L

F

F

H

H

W
|
W

G

G

L

P

H

L

A

P

W

W

A

A

I

T

Y

Y

A

S

I

F

V

L

A

S

L

V

C

A

I

F

A

A

Y

Y

F

F

K

F

F

F

-

V

R

R

K

K

D

M

A

E

P

V

G

I

L

R

I

P

S

S

T

T

L

L

Y

V

P

P

V

L

F

V

G

G

N

E

R

K

-

H

I

A

H

K

G

G

W

L

I

F

G

G

K

T

T

I

I

V

D

D

C

N

I

F

A

Y

V

L

F

V

A

A

T

L

V

I

V

F

G

A

V

M

A

G

T

T

S

S

L

L

G

G

L

L

G

A

A

T

A

P

Q

L

I

V

N

T

G

E

G

C

L

M

N

Q

Y

W

L

L

F

F

G

G

I

I

P

P

N

H

N

T

F

L

L

Q

T

L

Q

D

L

A

I

I

A

T

I

C

V

W

T

I

V

I

L

L

F

N

L

A

L

I

S

C

A

V

W

A

S

C

G

G

I

L

G

Q

K

K

G

A

I

V

K

R

Y

I

L

A

S

S

N

D

S

V

N

R

M

S

I

Y

F

L

A

S

A

F

L

L

L

M

M

L

I

G

F

W

L

V

L

F

I

I

V

V

G

S

P

G

T

A

V

S

Y

F

I

I

L

M

N

N

S

Y

F

F

T

T

D

D

S

S

I

V

G

G

Q

M

Y

L

L

L

S

M

N

Y

I

L

V

P

Q

R

M

M

S

L

F

F

R

Y

L

T

S

D

P

P

N

I

D

-

P

-

E

A

K

K

R

G

E
x
G

W

F

I

P

N

Q

G
|
G

W

W

T

T

I

V

F

F

Y

Y

W

W

A

A

W
|
W

W

W

I

V

S

I

W
x
Y

S

A

P

I

F

Q

V
x
M

G

S

I

I

F

F

I

L

A

A

R

R

V

I

S

S

R

R

G

G

R

R

T

T

I

V

R

R

E

E

F

L

L

C

I

F

G

G

V

M

L

V

V

L

A

G

P

L

S

T

I

A

L

S

V

T

F

W

L

I

W

L

F

W

S

T

I

V

F

L

G

G

V

S

S

N

A

T

M

L

D

L

L

L

Q

I

Q

D

N

K

N

N

I

I

V

L

D

N

V

I

A

P

N

N

M

L

S

I

T

E

E

Q

T

Y

M

G

L

V

F

A

G

R

V

A

L

I

N

I

E

E

-

T

-

W

-

A

Y

L

P

P

L

L

A

S

M

T

I

A

T

T

S

M

I

W

L

G

A

F

L

F

I

I

L

L

I

C

A

F

V

I

F

A

F

T

I

V

T

T

S

L

A

V

D

N

S

A

A

C

T

S

F

Y

V

T

L

L

G

A

M

M

Q

S

T

T

T

C

-

R

-

E

-

V

Y

R

G

D

S

G

L

E

N

D

P

P

A

P

N

L

S

L

V

V

K

R

I

I

S

G

W

W

G

S

I

I

I

L

Q

V

S

G

A

I

V

I

A

G

A

I

V

V

L

L

F

A

S

L

G

G

L

L

Q

K

A

P

L

I

Q
|
Q

N

T

T

A

A

I

K

I

L

A

A

G

L

C

P

P

F

L

S

F

V

F

V

V

I

N

I

I

L

M

M

V

I

T

V

L

S

S

L

F

Y

I

K

K

S

D

L

A

N

K

E

Q

E

N

R

W

R

K

binding 3-carboxy-n,n,n-trimethylpropan-1-aminium: W135 (= W137), G304 (≠ E306), G308 (= G310), W316 (= W318), Y320 (≠ W322), M324 (≠ V326), Q466 (= Q461)

Query Sequence

>WP_035702077.1 NCBI__GCF_000691145.1:WP_035702077.1
MKNVSSVFWIVIAITFIAVLWGALSPDTLQNVTDQIQTYITNDFGWYYLLVVSLLVGFCL
FFIFSPIGKITLGKPGEEPEFGLFSWFAMLFSAGMGIGLVFYGAAEPISHFAIQSPTGET
ENAQAFRDSLRYTFFHWGLHAWAIYAIVALCIAYFKFRKDAPGLISSTLYPVFGNRIHGW
IGKTIDCIAVFATVVGVATSLGLGAAQINGGLNYLFGIPNNFLTQLIIIAIVTVLFLLSA
WSGIGKGIKYLSNSNMIFAALLMIFLLIVGPTVYILNSFTDSIGQYLSNIVQMSFRLSPN
DPEKREWINGWTIFYWAWWISWSPFVGIFIARVSRGRTIREFLIGVLVAPSILVFLWFSI
FGVSAMDLQQNNIVDVANMSTETMLFGVLNEYPLAMITSILALILIAVFFITSADSATFV
LGMQTTYGSLNPANSVKISWGIIQSAVAAVLLFSGGLQALQNTAKLAALPFSVVIILMIV
SLYKSLNEERRAIKQANKYNKPRSPRVKKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory