SitesBLAST
Comparing WP_035702262.1 NCBI__GCF_000691145.1:WP_035702262.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8cqxA Ribokinase from t.Sp mutant a92g
43% identity, 98% coverage: 4:289/292 of query aligns to 2:295/300 of 8cqxA
- binding adenosine-5'-diphosphate: N179 (= N183), T217 (= T210), G219 (= G212), A220 (≠ K213), G222 (= G215), F250 (= F244), N272 (= N266), G275 (≠ A269), A276 (≠ S270), T279 (≠ V273)
- binding magnesium ion: D242 (= D236), T244 (= T238), A278 (≠ S272), S287 (≠ G281)
6znxC Ribokinase from thermus species
41% identity, 98% coverage: 4:289/292 of query aligns to 2:260/265 of 6znxC
4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
39% identity, 99% coverage: 1:289/292 of query aligns to 1:299/306 of 4xckA
- active site: A249 (≠ G239), A250 (= A240), G251 (= G241), D252 (= D242)
- binding adenosine-5'-diphosphate: T220 (= T210), G222 (= G212), S223 (≠ K213), V242 (= V232), T247 (= T237), A250 (= A240), F254 (= F244), H276 (≠ N266), A279 (= A269), V283 (= V273)
- binding alpha-D-ribofuranose: N11 (≠ S11), D13 (= D13), G39 (= G39), K40 (= K40), N43 (= N43), A95 (= A95), I107 (= I106), I109 (≠ V108), E140 (= E139), T248 (= T238), D252 (= D242)
P0DX97 Deoxyribokinase; dRK; ATP:2-deoxy-D-ribose 5-phosphotransferase; EC 2.7.1.229 from Salmonella typhi (see paper)
35% identity, 98% coverage: 4:288/292 of query aligns to 3:298/306 of P0DX97
- M10 (≠ S11) Important for substrate specificity; mutation to N: 2.5-fold decrease in Vmax for deoxyribokinase activity and 70-fold increase in KM for deoxyribose. 2-fold increase in KM for ribose.
1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
39% identity, 98% coverage: 4:289/292 of query aligns to 4:299/305 of 1rk2A
- active site: A249 (≠ G239), A250 (= A240), G251 (= G241), D252 (= D242)
- binding adenosine-5'-diphosphate: T220 (= T210), G222 (= G212), S223 (≠ K213), A250 (= A240), G251 (= G241), H276 (≠ N266), A279 (= A269)
- binding tetrafluoroaluminate ion: G213 (≠ P204), R215 (≠ K206)
- binding magnesium ion: D246 (= D236), A282 (≠ S272), R285 (≠ K275), S291 (≠ G281)
- binding alpha-D-ribofuranose: N11 (≠ S11), D13 (= D13), G38 (= G38), G39 (= G39), K40 (= K40), N43 (= N43), E140 (= E139), D252 (= D242)
1gqtB Activation of ribokinase by monovalent cations (see paper)
39% identity, 98% coverage: 4:289/292 of query aligns to 6:301/307 of 1gqtB
- active site: A251 (≠ G239), A252 (= A240), G253 (= G241), D254 (= D242)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T210), G224 (= G212), S225 (≠ K213), A252 (= A240), G253 (= G241), H278 (≠ N266), A281 (= A269)
- binding cesium ion: D248 (= D236), I250 (≠ T238), A284 (≠ S272), R287 (≠ K275), S293 (≠ G281)
- binding alpha-D-ribofuranose: N13 (≠ S11), D15 (= D13), G41 (= G39), N45 (= N43), E142 (= E139), D254 (= D242)
P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 98% coverage: 4:289/292 of query aligns to 7:302/309 of P0A9J6
A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 98% coverage: 4:288/292 of query aligns to 71:371/379 of A1A6H3
Sites not aligning to the query:
- 1:74 mutation Missing: Loss of ribokinase activity.
6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
36% identity, 98% coverage: 4:288/292 of query aligns to 5:305/313 of 6ilsB
- binding adenosine-5'-triphosphate: N189 (= N183), K225 (≠ T210), G227 (= G212), I246 (≠ V227), A248 (≠ G229), A257 (= A240), G258 (= G241), F261 (= F244), A286 (= A269), S287 (= S270)
- binding alpha-D-ribofuranose: N12 (≠ S11), D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), E144 (= E139), D259 (= D242)
6wjzA Crystal structure of human ribokinase in complex with ampcp
36% identity, 99% coverage: 1:288/292 of query aligns to 2:304/315 of 6wjzA
- binding phosphomethylphosphonic acid adenosyl ester: N186 (= N183), T222 (= T210), G224 (= G212), A225 (≠ K213), G227 (= G215), T243 (≠ G229), V246 (= V232), A254 (= A240), G255 (= G241), N282 (= N266), A285 (= A269), A286 (≠ S270), V289 (= V273)
5c3yA Structure of human ribokinase crystallized with amppnp
36% identity, 99% coverage: 1:288/292 of query aligns to 1:303/306 of 5c3yA
- active site: G252 (= G239), A253 (= A240), G254 (= G241), D255 (= D242)
- binding amp phosphoramidate: T221 (= T210), G223 (= G212), V245 (= V232), T250 (= T237), G254 (= G241), N281 (= N266), A284 (= A269), A285 (≠ S270)
6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
36% identity, 99% coverage: 1:288/292 of query aligns to 2:304/311 of 6wk0B
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T210), G224 (= G212), A225 (≠ K213), G227 (= G215), T243 (≠ G229), V246 (= V232), A254 (= A240), G255 (= G241), N282 (= N266), A285 (= A269), A286 (≠ S270), V289 (= V273)
- binding alpha-D-ribofuranose: D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), A96 (= A95), E141 (= E139), D256 (= D242)
5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
36% identity, 99% coverage: 1:288/292 of query aligns to 2:304/317 of 5c41A
- active site: G253 (= G239), A254 (= A240), G255 (= G241), D256 (= D242)
- binding phosphomethylphosphonic acid adenylate ester: N186 (= N183), T222 (= T210), G224 (= G212), A225 (≠ K213), G227 (= G215), V246 (= V232), G255 (= G241), N282 (= N266), A285 (= A269), A286 (≠ S270)
2fv7A Crystal structure of human ribokinase
36% identity, 99% coverage: 1:288/292 of query aligns to 1:303/308 of 2fv7A
- active site: G252 (= G239), A253 (= A240), G254 (= G241), D255 (= D242)
- binding adenosine-5'-diphosphate: N185 (= N183), T221 (= T210), G223 (= G212), G226 (= G215), T242 (≠ G229), V245 (= V232), A253 (= A240), G254 (= G241), N281 (= N266), A284 (= A269), A285 (≠ S270), V288 (= V273)
6a8cA Ribokinase from leishmania donovani with adp (see paper)
37% identity, 98% coverage: 4:288/292 of query aligns to 16:322/327 of 6a8cA