SitesBLAST

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
26% identity, 72% coverage: 4:390/539 of query aligns to 8:384/469 of P46349

query
sites
P46349

L

L

H

K

R

K

R

E

M

L

G

K

T

T

F

R

A

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M

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G
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G

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G

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G
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G

P

P

A

G

A

A

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Y

L

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E

L

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I

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L

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-

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M

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-

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N

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I

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A

L

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L

L

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G

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H

A

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A

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P

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E

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N

F

L

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E

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L

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A

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A

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A

A

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A

A

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D

L

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M

-

I

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-

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W

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A

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N

-

I

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D

A

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A

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M

N

N

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F

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L

L

I

-

L

-

Y

-

A

-

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A

A

F

V

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L

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C

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L

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G
|
G

I

L

T

Y

Y

-

T

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A

-

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T

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S

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R

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M

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Y

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L

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P

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-

Y

K

G

G

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V

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Q

A

A

M

I

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A

N
x
G

L

T

G

F

V
x
F

S

S

F

Y

I

I

-

A

-

V

-

M

-

N

-

Y

-

F

-

S

-

P

-

D

-

T

-

V

F

F

L

L

F

F

L

L

F

V

R

N

G

S

W

S

G

G

V

A

L

I

A

A

E

-

I

-

S

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V

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A

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-

L

L

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Y

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K

G33 (= G29) mutation to D: Lack of activity.
G42 (= G38) mutation to S: Lack of activity.
G301 (= G308) mutation to V: Lack of activity.
G338 (≠ N349) mutation to E: Lack of activity.
F341 (≠ V352) mutation to S: Lack of activity.

Sites not aligning to the query:

414 G→R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
25% identity, 64% coverage: 4:347/539 of query aligns to 13:350/489 of P25737

query
sites
P25737

L

L

H

R

R

R

R

E

M

L

G

K

T

A

F

R

A

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L

L

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M

M

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I

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A

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I

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L

L

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G

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G

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A

A

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I

Q

A

A

G

G

P

P

A

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-

G

A

A

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L

L

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Y

L

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L

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M

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L

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L

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E

L

L

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A

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G

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M

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Y

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Y

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S

Y

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G

E

S

E

F

G

I

F

G

G

F

F

I

A

A

L

A

G

W

W

A

N

N
x
Y

W

W

I

Y

A

N

I
x
W

V

A

S

V

V

T

I

I

P

A

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V

E

D

A

L

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A

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A

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Q

L

Y

V

M

M

S

S

S

W

W

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P

-

W

-

E

-

W

-

A

-

K

-

W

-

T

-

S

-

H

-

L

-

V

-

T

-

K

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G

-

V

F

L

P

T

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P

G

G

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M

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W

A

S

S

A

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L

L

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I

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F

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L

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N

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Y

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E

A

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K

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E

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L

L

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A

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P

A

Y

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T

D

D

P

P

S

S

M

L

I

L

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R

N

N

G
x
D

W

V

G

K

H
x
D

L

I

N

S

F

V

N

-

S

S

P

P

F

F

A

T

D

L

L

V

A

F

I

Q

A

H

L

A

G

G

I

L

N

L

W

S

L

A

V

A

L

A

I

V

L

M

Y

N

A

A

D

V

A

I

F

L

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S

A

P

V

S

L

G

S

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A

G

G

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N

T

S

Y

G

T

M

A

Y

T

A

T

S

S

T

R

R

M

M

I

L

Y

Y

G

T

M

L

E

A

K

C

N

D

K

G

Y

K

M

A

P

P

S

R

V

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L

F

G

A

R

K

L

L

H

S

P

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Y

G

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N

A

A

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L

F

Y

Y102 (≠ N92) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (≠ I96) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K168) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F214) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (≠ Q220) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E228) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ G270) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (≠ H273) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
25% identity, 72% coverage: 3:389/539 of query aligns to 27:431/629 of P30825

query
sites
P30825

H

R

L

L

H

S

R

R

R

C

M

L

G

N

T

T

F

F

A

D

L

L

M

V

M

A

V

L

G

G

L

V

G

G

S

S

I

T

I

L

G

G

S

A

G

G

-

V

W

Y

L

V

F

L

G

A

A

G

W

A

R

V

A

A

A

R

Q

E

I

N

A

A

G

G

P

P

A

A

A

I

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L

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S

S

W

F

L

L

I

I

G

A

M

A

V

L

V

A

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L

V

F

L

I

A

A

G

L

L

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C

Y

Y

S

G

E

E

L

F

G

G

S

A

M

R

F

V

P

P

E

K

A

T

G

G

G

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M

A

V

Y

K

L

Y

Y

T

S

Q

Y

Y

V

S

T

H

V

G

G

S

E

F

L

I

W

G

A

F

F

I

I

A

T

A

G

W

W

A

N

-

L

-

I

-

L

N

S

W

Y

I

I

A

I

I

G

V

T

S

S

V

S

I

V

P

A

V

R

E

A

A

W

V

S

A

A

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F

Q

D

Y

E

M

L

S

I

S

G

W

R

P

P

-

I

W

G

E

E

W

F

A

S

K

R

W

T

T

H

S

M

H

T

L

L

V

N

T

A

K

P

G

G

V

V

L

L

T

A

T

E

E

N

G

P

L

D

M

I

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F

A

A

S

V

V

I

L

I

V

L

I

I

Y

L

F

T

L

G

L

L

N

L

Y

T

W

L

T

G

V

V

G

K

L

E

F

S

S

A

K

M

A

V

N

N

T

K

F

I

I

F

T

T

I

C

F

I

K

N

-

V

-

L

-

V

-

L

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G

-

F

I

I

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M

I

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P

S

G

G

L

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K

G

G

A

S

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F

N

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G

Q

F

L

H

T

S

E

E

E

N

D

F

F

-

G

-
x
N

T

T

S

S

G

G

T

R

-

L

-

C

-

L

-

N

-

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

-

V

-

G

S

G

I

F

A

M

P

P

N

F

G

G

W

F

A

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S

G

V

V

L

L

T

S

A

G

V

A

A

A

T

T

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C

G

-

I

-

V

F

F

Y

A

A

F

F

N

V

G

G

F

F

Q

D

S

C

P

I

I

A

N

T

M

T

A

G

G

E

E

E

A

V

K

K

N

N

P

P

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R

K

S

A

I

I

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P

I

V

A

G

I

I

V

V

G

A

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S

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L

L

L

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A

C

T

F

V

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I

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Y

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L

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Q

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A

A

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Y

I

F

G

G

A

V

V

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D

A

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A

S

L

M

T

I

L

V

M

N

M

G

P

W

Y

G

F

H

C

L

L

N

D

F

N

N

N

S

S

P

P

F

L

A

P

D

D

L

A

A

F

I

K

A

H

L

V

G

G

I

W

N

E

W

G

L

A

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K

L

Y

I

A

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Y

A

A

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D

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A

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F

L

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A

P

L

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S

G

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T

G

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F

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P

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M

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P

R

R

M

V

I

I

Y

Y

G

A

M

M

E

A

K

E

N

D

K

G

Y

L

M

L

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F

S

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F

L

L

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A

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N

L

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N

P

D

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Y

T

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P

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P

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A

A

M

T

F

L

F

A

N

S

L

G

G

A

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V

S

A

F

A

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V

F

M

L

A

F

F

L

L

F

F

R

D

G

-

W

L

G

K

V

D

L

L

A

V

E

D

I

L

I

M

S

S

V

I

A

G

T

T

L

L

I

L

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A

Y

Y

L

S

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L

G

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P

A

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A

T

C

V

V

M

L

T

V

L

L

R

R

N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
25% identity, 63% coverage: 4:342/539 of query aligns to 7:333/467 of Q88CZ8

query
sites
Q88CZ8

L

L

H

K

R

R

R

G

M

L

G

S

T

A

F

R

A

H

L

I

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R

M

F

V

M

G

A

L

L

G

G

S

S

I

A

I

I

G

G

S
x
T

G

G

W

L

L

F

F

Y

G

G

A

S

W

A

R

S

A

A

A

I

Q

Q

I

M

A

A

G

G

P

P

A

A

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I

L

L

L

S

A

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Y

L

L

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I

G

G

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A

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F

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I

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W

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x
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H

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K

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M

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P

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N

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A

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S

-

G

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I

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F
|
F

A

A

F

F

N

G

G

G

F

I

Q
x
E

S

I

P

I

I

G

N

V

M

T

A

A

G

G

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E

A

A

K

K

N

D

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P

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L

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Y

Y

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V

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L

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G

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A

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M

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W

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H

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N

G

F

S

N

Q

-

G

S

S

P

P

F

F

A

V

D

Q

L

I

A

F

I

S

A

N

L

L

G

G

I

I

N

G

W

S

L

A

V

A

L

A

I

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L

L

Y

N

A

V

D

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A

V

F

I

V

S

S

A

P

A

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I

G

S

T

A

G

I

I

N

T

S

Y

D

T

I

A

F

T

G

T

A

S

G

R

R

M

M

I

M

Y

Y

G

G

M

L

E

A

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Q

N

Q

K

G

Y

H

M

A

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P

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V

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L

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R

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L

L

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I

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Y

H

G

G

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V

P

P

T27 (≠ S24) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ A95) mutation to A: Retains 80% of wild-type activity.
K156 (= K168) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F214) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (≠ Q220) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

Q7YQK4 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; L-type amino acid transporter 1; LAT1; Solute carrier family 7 member 5 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
24% identity, 83% coverage: 4:450/539 of query aligns to 42:480/503 of Q7YQK4

query
sites
Q7YQK4

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

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M

V

M

A

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F

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A

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A

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A

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A

A

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G

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A

P

-

G

-

L

A

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I

L

L

V

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W

W

L

A

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G
x
C

M

G

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V

V

F

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I

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A

A

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x
C

Y

Y

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A

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E

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L

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K

A

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K

A

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Y

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Y

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G

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S

F

L

I

P

G

A

F

F

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A

K

A

L

W

W

A

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N

-

W

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I

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A

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P

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-

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Q

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C

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A

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x
C

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L
x
C

L

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V

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A

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N

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x
C

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|
W

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A

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F

A

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F

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-

W

-

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C

-

V

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A

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G

A

P

I

V

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T

G

V

M

M

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T

W

L

L

R

R

K

Y

T

K

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

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F

F

F

V

I

F

L

A

A

S
x
C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T
x
C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

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Y

W

Q

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A

N

K

K

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P

K

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W

C88 (≠ G48) mutation to S: No significant effect on inhibition by HgCl(2). Decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-183.
C98 (≠ S58) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Slightly less decreased KM and Vmax for Phe; when associated with S-183.
C160 (≠ K128) mutation to S: No change to KM or Vmax for Phe.
C172 (≠ S140) mutation to S: No change to KM or Vmax for Phe.
C174 (≠ L142) mutation to S: No change to KM or Vmax for Phe.
C183 (≠ Y151) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-88. Slightly less decreased KM and Vmax for Phe; when associated with S-98.
G219 (vs. gap) mutation to D: Decreased KM and Vmax for Trp. Increased KM and Vmax for Phe; when associated with L-234.
W234 (= W200) mutation to L: Decreased KM and Vmax for Trp. Increased KM but decreased Vmax for Phe. Increased KM and Vmax for Phe; when associated with D-219.
C331 (≠ A300) mutation to S: No significant effect on inhibition by HgCl(2). Increased KM and Vmax for Phe.
C377 (≠ L350) mutation to S: No significant effect on inhibition by HgCl(2).
C403 (vs. gap) mutation to S: No significant effect on inhibition by HgCl(2).
C439 (≠ S416) mutation to S: Prevents insertion into the plasma membrane and possibly protein folding.
C454 (≠ T427) mutation to S: No significant effect on inhibition by HgCl(2). Slightly increased KM but slightly decreased Vmax for Phe.

Sites not aligning to the query:

492 C→S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe.

O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
25% identity, 83% coverage: 2:447/539 of query aligns to 6:435/438 of O34739

query
sites
O34739

N

N

H

G

L

L

H

K

R

K

R

E

M

I

G

G

T

L

-

L

F

F

A

A

L

L

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M

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-

L

I

G

G

S

T

I

I

G

G

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S

G

G

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F

F

M

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-

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G

A

A

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A

A

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Q

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A

D

G

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A

M

A

A

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L

F

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A

W

W

L

L

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L

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G

M

G

V

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V

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I

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L

L

F

A

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A

G

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Y

I

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A

E

E

L

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P

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A

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G

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Y

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H

Y

G

G

S

E

F

F

I

W

G

G

F

F

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A

-

W

-

A
x
C

N

G

W

W

I

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A

Q

I

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I

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I

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Y

I

G

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P

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A

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A

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G

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M

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S

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W

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A

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G

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W

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G

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S

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G

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S

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K

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G

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A

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L

G

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L

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x
C

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V

A

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N

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Y

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I

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C

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F

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K

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N

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x
C

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N

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F

L

M

S

P

I

I

I

L

A

A

P

I

L

A

G

G

F

S

V

F

F

F

A

V

S

L

L

G

T

S

L

T

Y

L

W

I

A

T

R

D

W

T

P

M

L

S

T
x
C

G

G

Q

L

V

S

L

I

F

L

I

I

I

G

L

L

I

A

G

G

L

L

P

P

I

V

Y

Y

F

Y

Y

G

Y

M

Q

K

A

K

K

R

C94 (≠ A91) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
C141 (≠ M137) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
C168 (≠ K168) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
C291 (≠ S303) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
C415 (≠ T427) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
25% identity, 76% coverage: 2:411/539 of query aligns to 9:405/457 of P15993

query
sites
P15993

N

E

H

Q

L

L

H

K

R

R

R

G

M

L

G

K

T

N

F

R

A

H

L

I

M

Q

M

L

V

I

G

A

L

L

G

G

S

G

I

A

I

I

G

G

S

T

G

G

W

L

L

F

F

L

G

G

A

S

W

A

R

S

A

V

A

I

Q

Q

I

S

A

A

G

G

P

P

A

G

A

I

I

I

L

L

S

G

W

Y

L

A

I

I

G

A

M

G

V

F

V

I

I

A

L

F

F

L

I

I

A

M

L

R

S

Q

Y

L

S

G

E

E

L

M

G

V

S

V

M

E

F

E

P

P

E

V

A

A

G

G

G

S

M

F

V

S

K

H

Y

F

T

A

Q

Y

Y

K

S

Y

H

W

G

G

S

S

F

F

I

A

G

G

F

F

I

A

A

S

A

G

W

W

A

N

N

Y

W

W

I

V

A

L

I
x
Y

V

V

S

L

V

V

I

A

P

M

V

A

E

E

A

L

V

T

A

A

S

V

V

G

Q

K

Y

Y

M

I

S

Q

S

F

W

W

P

Y

W

P

E

E

W

I

A

P

K

T

W

W

T

V

S

S

H

-

L

-

V

-

T

-

K

-

G

-

V

-

L

-

T

-

E

-

G

-

L

-

M

-

V

-

A

A

S

A

V

V

L

F

V

V

I

V

Y

I

F

N

L

A

L

I

N

N

Y

L

W

T

T

N

V

V

G

K

L

V

F

F

S

G

K

E

A

M

N

E

T

F

F

W

I

F

T

A

I

I

F

I

K

K

I

V

V

I

-

A

I

V

P

V

G

A

L

M

T

I

I

I

-

F

-

G

G

G

A

W

L

L

F

F

V

S

G

G

F

N

H

G

S

G

E

P

N

Q

F

A

T

T

S

V

G

S

T

N

-

L

-

W

-

D

-

Q

-

G

S

G

I

F

A

L

P

P

N

H

G

G

W

F

A

T

S

G

V

L

L

V

T

M

A

M

V

M

A

A

T

I

S

-

G

-

I

I

V

M

F

F

A

S

F

F

N

G

G

G

F

L

Q

E

S

L

P

V

I

G

N

I

M

T

A

A

G

A

E

E

A

A

K

D

N

N

P

P

G

E

R

Q

S

S

I

I

P

P

I

K

A

A

I

-

V

-

G

-

S

-

I

-

L

-

I

T

A

N

T

Q

V

V

I

I

Y

Y

V

R

L

I

L

L

Q

-

I

I

A

F

F

Y

I

I

G

G

A

S

V

L

D

A

P

V

S

L

M

L

I

S

V

L

N

M

G

P

W

W

G

T

H

R

L

V

N

T

F

A

N

D

-

T

S

S

P

P

F

F

A

V

D

L

L

I

A

F

I

H

A

E

L

L

G

G

I

-

N

-

W

-

L

-

V

-

L

-

I

-

L

-

Y

-

A

-

D

D

A

T

F

F

V

V

S

A

P

N

S

A

G

L

T

N

G

I

I

V

T

V

Y

L

T

T

A

A

-

A

-

L

-

S

-

V

-

Y

-

N

-

S

-

C

-

V

-

Y

T

C

T

N

S

S

R

R

M

M

I

L

Y

F

G

G

M

L

E

A

K

Q

N

Q

-

G

-

N

-

A

-

P

K

K

Y

A

M

L

P

A

S

S

V

V

L

D

G

K

R

R

L

G

H

V

P

P

I

V

Y

N

G

T

V

I

P

L

R

V

P

S

A

A

M

L

F

V

F

T

N

A

L

L

G

C

V

V

S

L

F

I

I

N

F

Y

L

L

F

A

L

P

F

E

R

S

G

A

W

F

G

G

V

L

L

L

A

M

E

A

I

L

I

V

S

V

V

S

A

A

T

L

L

V

I

I

S

N

Y

W

L

A

T

M

G

I

P

S

V

L

T

A

V

H

M

M

T

K

L

F

R

R

K

R

T

A

G

K

T

Q

D

E

L

Q

Y

G

R

V

P

V

L

T

R

R

I

F

K

P

G

A

L

L

S

-

I

-

I

L

A

Y

P

P

L

L

G

G

Y103 (≠ I96) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
23% identity, 74% coverage: 4:404/539 of query aligns to 19:408/458 of P24207

query
sites
P24207

L

L

H

H

R

R

R

G

M

L

G

H

T

N

F
x
R

A

H

L

I

M

Q

M

L

V

I

G

A

L

L

G

G

S

G

I

A

I

I

G

G

S

T

G

G

W

L

L

F

F

L

G

G

A

I

W

G

R

P

A

A

A

I

Q

Q

I

M

A

A

G

G

P
|
P

A

A

A

V

I

L

L

L

S

G

W

Y

L

G

I

V

G

A

M

G

V

I

I

A

L

F

F

L

I

I

A

M

L

R

S

Q

Y

L

S

G

E

E

L

M

G

V

S

V

M

E

F

E

P

P

E

V

A

S

G

G

G

S

M
x
F

V

A

K

H

Y
x
F

T

A

Q
x
Y

Y

K

S
x
Y

H
x
W

G

G

S

P

F
|
F

I

A

G

G

F
|
F

I

L

A

S

A

G

W
|
W

A

N

N
x
Y

W
|
W

I

V

A

M

I
x
F

V

V

S

L

V

V

I

G

P

M

V

A

E
|
E

A

L

V

T

A

A

S

A

V

G

Q

I

Y

Y

M

M

S

Q

S

Y

W

W

P

F

W

P

E

D

W

V

A

P

K

T

W

W

T

-

S

-

H

-

L

-

V

-

T

-

K

-

G

-

V

-

L

-

T

-

E

-

G

-

L

-

M

I

V

W

A

A

S

A

V

A

L

F

V

I

I

I

Y

I

F

N

L

A

L

V

N

N

Y

L

W

V

T

N

V

V

G

R

L

L

F

Y

S

G

K

E

A

T

N

E

T

F

F

W

I

F

T

A

I

L

F

I

K
|
K

I

V

V

L

I

A

P

I

G

I

L

G

T

M

I

I

G

G

A

F

-

G

-

L

-

W

L

L

F

F

V

S

G

G

F

H

H

G

S

G

E

E

-

K

-

A

-

S

-

I

-

D

N

N

F

L

T

W

S

R

G

Y

T

G

S

G

I

F

A

F

P

A

N

T

G

G

W

W

A

N

S

G

V

L

L

I

T

L

A

S

V

L

A

A

T

V

S

-

G

-

I

I

V

M

F

F

A

S

F

F

N

G

G

G

F

L

Q
x
E

S

L

P

I

I

G

N

I

M

T

A

A

G

A

E

E

A

A

K

R

N

D

P

P

G

E

R

K

S

S

I

I

P

P

I

K

A

A

I

-

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-

G

-

S

-

I

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L

-

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A

N

T

Q

V

V

I

V

Y

Y

V
x
R

L

I

L

L

Q

-

I

L

A

F

F

Y

I

I

G

G

A

S

V

L

D

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V

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L

M

L

I

A

V

L

N

Y

G

P

W

W

G

V

H

E

L

V

N

K

F

S

N

N

-

S

S

S

P

P

F

F

A

V

D

M

L

I

-

F

-

H

-

N

-

L

-

D

-

S

-

N

A

V

I

V

A

A

L

S

G

A

I

L

N

N

W

F

L

V

V

I

L

L

I

V

L

-

Y

-

A

-

D

-

A

A

F

S

V

L

S

S

P

V

S

Y

G

N

T

S

G

G

I

V

T

Y

Y

-

T

-

A

-

T

S

T

N

S

S

R

R

M

M

I

L

Y

F

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G

M

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K

G

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M

A

P

P

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V

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L

L

G

T

R

R

L

V

H

S

-

R

-

G

-

V

P
|
P

I

I

Y

N

G

S

V

L

P

M

R

L

P

S

A

G

M

A

F

I

F

T

N

S

L

L

G

V

V

V

S

L

F

I

I

N

F

Y

L

L

F

L

L

P

F

Q

R

K

G

A

W

F

G

G

V

L

L

L

A

M

E

A

I

L

I

V

S

V

V

A

A

T

T

L

L

L

I

L

S

N

Y

W

L

I

T

M

G

I

P

C

V

L

T

A

V

H

M

L

T

R

L

F

R

R

K

A

T

A

G

M

T

R

D

R

L

Q

Y

G

R

R

P

E

L

T

R

Q

I

F

K

K

G

A

L

L

R26 (≠ F11) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P39) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (≠ M72) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ Y75) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ Q77) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ S79) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ H80) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (= F83) mutation to L: No effect on phenylalanine transport activity.
F101 (= F86) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W90) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (≠ N92) mutation to L: No effect on phenylalanine transport activity.
W108 (= W93) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ I96) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E103) mutation E->G,L,V,N: Loss of activity.
K168 (= K168) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (≠ Q220) mutation E->A,Q,K,R,W: Loss of activity.
R252 (≠ V252) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P337) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 55% coverage: 44:342/539 of query aligns to 58:340/461 of P76037

query
sites
P76037

S

S

W

Y

L

L

I

L

G

A

M

L

V

A

V

G

I

V

L

L

F

F

I

T

A

A

L

I

S

S

Y

Y

S

G

E

K

L

L

G

V

S

R

M

Q

F

F

P

P

E

E

A

A

G

G

G

S

M

A

V

Y

K

T

Y

Y

T

A

Q

Q

Y

K

S

S

H

I

G

N

S

P

F

H

I

V

G

G

F

F

I

M

A

V

A

G

W

W

A

S

N

S

-

L

-

D

-
x
Y

-

L

W

F

I

L

A

P

I

M

V

I

S

N

V

V

I

L

P

L

V

A

E

K

A

I

V

Y

A

L

S

S

V

A

Q

L

Y

F

M

P

S

E

S

V

W

P

P

P

W

W

E

V

W

W

A

-

K

-

W

-

T

-

S

-

H

-

L

-

V

-

T

-

K

-

G

-

V

V

L

V

T

T

T

F

E

V

G

A

L

I

M

L

V

T

A

A

S

A

V

N

L

L

L

K

V

S

I

V

Y

N

F

L

L

V

L

A

N

N

Y

F

W

N

T

T

V

L

G

F

L

V

F

L

S

V

K

Q

A

I

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S

T

I

F

M

I

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V

I

F

F

I

K

F

I

L

V

V

I

V

P

Q

G

G

L

L

T

H

I

K

G

G

A

E

L

G

L

V

F

G

V

T

G

V

F

W

H

S

S

L

E

Q

N

P

F

F

T

I

S

S

G

E

T

N

S

-

I

-

A

-

P

-

N

-

G

-

W

-

A

A

S

H

V

L

L

I

T

P

A

I

V

I

A

T

T

G

S

A

G

T

I

I

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-

C

F

F

A

S

F

F

N

L

G

G

F

F

Q

D

S

A

P

V

I

T

N

T

M

L

A

S

G

E

E

E

A

T

K

P

N

D

P

A

G

A

R

R

S

V

I

I

P

P

I

K

A

A

I

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V

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L

S

T

I

A

L

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I

Y

A

G

T

G

V

V

I

I

Y

F

V

I

L

A

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S

I

F

A

-

F

F

I

M

G

Q

A

L

V

F

D

F

P

P

S

D

M

I

I

S

V

-

N

-

G

-

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-

G

-

H

-

L

-

N

R

F

F

N

K

S

D

P

P

F

D

A

A

-

A

-

L

-

P

D

E

L

I

A

A

I

L

A

Y

L

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G

G

I

G

N

K

W

L

L

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L

S

I

I

L

F

Y

L

A

C

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T

A

T

F

F

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V

S

N

P

T

S

L

G

A

T

S

G

G

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L

T

A

Y

S

T

H

A

A

T

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T

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S

S

R

R

M

L

I

L

Y

Y

G

V

M

M

E

G

K

R

N

D

K

N

Y

V

M

F

P

P

S

E

-

R

V

V

L

F

G

G

R

Y

L

V

H

H

P

P

I

K

Y

W

G

R

V

T

P

P

Y110 (vs. gap) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

8j8mB Overall structure of the lat1-4f2hc bound with tryptophan
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/463 of 8j8mB

query
sites
8j8mB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S

T

I
|
I

I

I

G
|
G

S
|
S

G

G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V

I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M

I

S

V

S

A

W

L

P

V

W

F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W

Y

T

S

V

V

G

-

L

-

F

-

S

-

K

K

A

A

N

A

T

T

F

R

I

V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F
|
F

A

A

F

Y

N

G

G

G

F

W

Q

N

S

Y

P

L

I

N

N

F

M

V

A

T

G

E

E

E

A

M

K

I

N

N

P

P

G

Y

R

R

S

N

I

L

P

P

I

L

A

A

I

I

V

I

G

I

S

S

I

L

L

P

I

I

A

V

T

T

V

L

I

V

Y

Y

V

V

L

L

L

T

Q

N

I

L

A

A

F

Y

I

F

G

T

A

T

V

L

D

S

P

T

S

E

M

Q

I

M

V

L

N

S

G

S

W

E

G

A

-

V

H

A

L

V

N

D

F

F

N

G

S

N

P

-

F

-

A

-

D

-

L

-

A

-

I

Y

A

H

L

L

G

G

I

V

-

M

N

S

W

W

L

I

V

I

L

P

I

V

L

F

Y

V

A

G

-

L

D

S

A

C

F

F

V

G

S

S

P

V

S

N

G

G

T

S

G

-

I

-

T

-

Y

-

T

L

A

F

T

T

T

S

S

S

R

R

M

L

I

F

Y

F

G

V

M

G

E

S

K

R

N

E

K

G

Y

H

M

L

P

P

S

S

V

I

L

L

G

S

R

M

L

I

H

H

P

P

I

Q

Y

L

G

L

V

T

P

P

R

V

P

P

A

S

M

L

F

V

F

F

N

T

L

C

G

V

V

M

S

T

F

L

I

L

F

Y

L

A

F

F

L

-

F

-

R

-

G

-

W

-

G

-

V

-

L

S

A

K

E

D

I

I

I

F

S

S

V

V

A

I

T

N

L

F

I
x
F

S

S

Y

F

L

F

T

N

G

W

P

L

V

C

T

V

V

A

M

L

T

A

-

I

-

G

-

M

-

I

-

W

L

L

R

R

K

H

T

R

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

G

F

F

F

V

I

F

L

A

A

S

C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T

C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

F

-

G

-

V

-

W

Y

W

Q

K

A

N

K

K

A

P

K

K

W

W

binding tryptophan: I20 (= I21), G22 (= G23), S23 (= S24), F209 (= F214), F357 (≠ I376)

8j8lB Overall structure of the lat1-4f2hc bound with l-dopa
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/463 of 8j8lB

query
sites
8j8lB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S

T

I
|
I

I

I

G
|
G

S
|
S

G
|
G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V

I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M

I

S

V

S

A

W

L

P

V

W

F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W

Y

T

S

V

V

G

-

L

-

F

-

S

-

K

K

A

A

N

A

T

T

F

R

I

V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F
|
F

A

A

F

Y

N
x
G

G
|
G

F

W

Q

N

S

Y

P

L

I

N

N

F

M

V

A

T

G

E

E

E

A

M

K

I

N

N

P

P

G

Y

R

R

S

N

I

L

P

P

I

L

A

A

I

I

V

I

G

I

S

S

I

L

L

P

I

I

A

V

T

T

V

L

I

V

Y

Y

V

V

L

L

L

T

Q

N

I

L

A

A

F

Y

I

F

G

T

A

T

V

L

D

S

P

T

S

E

M

Q

I

M

V

L

N

S

G

S

W

E

G

A

-

V

H

A

L

V

N

D

F

F

N

G

S

N

P

-

F

-

A

-

D

-

L

-

A

-

I

Y

A

H

L

L

G

G

I

V

-

M

N

S

W

W

L

I

V

I

L

P

I

V

L

F

Y

V

A

G

-

L

D

S

A

C

F

F

V

G

S

S

P

V

S

N

G

G

T

S

G

-

I

-

T

-

Y

-

T

L

A

F

T

T

T

S

S

S

R

R

M

L

I

F

Y

F

G

V

M

G

E

S

K

R

N

E

K

G

Y

H

M

L

P

P

S

S

V

I

L

L

G

S

R

M

L

I

H

H

P

P

I

Q

Y

L

G

L

V

T

P

P

R

V

P

P

A

S

M

L

F

V

F

F

N

T

L

C

G

V

V

M

S

T

F

L

I

L

F

Y

L

A

F

F

L

-

F

-

R

-

G

-

W

-

G

-

V

-

L

S

A

K

E

D

I

I

I

F

S

S

V

V

A

I

T

N

L

F

I
x
F

S

S

Y

F

L

F

T

N

G

W

P

L

V

C

T

V

V

A

M

L

T

A

-

I

-

G

-

M

-

I

-

W

L

L

R

R

K

H

T

R

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

G

F

F

F

V

I

F

L

A

A

S

C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T

C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

F

-

G

-

V

-

W

Y

W

Q

K

A

N

K

K

A

P

K

K

W

W

binding 3,4-dihydroxyphenylalanine: I20 (= I21), G22 (= G23), S23 (= S24), G24 (= G25), F209 (= F214), G212 (≠ N217), G213 (= G218), F357 (≠ I376)

8x0wB Overall structure of the lat1-4f2hc bound with leu
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/464 of 8x0wB

query
sites
8x0wB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S
x
T

I
|
I

I

I

G
|
G

S
|
S

G

G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V

I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M

I

S

V

S

A

W

L

P

V

W

F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W

Y

T

S

V

V

G

-

L

-

F

-

S

-

K

K

A

A

N

A

T

T

F

R

I

V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F

F

A

A

F

Y

N
x
G

G

G

F

W

Q

N

S

Y

P

L

I

N

N

F

M

V

A

T

G

E

E

E

A

M

K

I

N

N

P

P

G

Y

R

R

S

N

I

L

P

P

I

L

A

A

I

I

V

I

G

I

S

S

I

L

L

P

I

I

A

V

T

T

V

L

I

V

Y

Y

V

V

L

L

L

T

Q

N

I

L

A

A

F

Y

I

F

G

T

A

T

V

L

D

S

P

T

S

E

M

Q

I

M

V

L

N

S

G

S

W

E

G

A

-

V

H

A

L

V

N

D

F

F

N

G

S

N

P

-

F

-

A

-

D

-

L

-

A

-

I

Y

A

H

L

L

G

G

I

V

-

M

N

S

W

W

L

I

V

I

L

P

I

V

L

F

Y

V

A

G

-

L

D

S

A

C

F

F

V

G

S

S

P

V

S

N

G

G

T

S

G

-

I

-

T

-

Y

-

T

L

A

F

T

T

T

S

S

S

R

R

M

L

I

F

Y

F

G

V

M

G

E

S

K

R

N

E

K

G

Y

H

M

L

P

P

S

S

V

I

L

L

G

S

R

M

L

I

H

H

P

P

I

Q

Y

L

G

L

V

T

P

P

R

V

P

P

A

S

M

L

F

V

F

F

N

T

L

C

G

V

V

M

S

T

F

L

I

L

F

Y

L

A

F

F

L

-

F

-

R

-

G

-

W

-

G

-

V

-

L

S

A

K

E

D

I

I

I

F

S

S

V

V

A

I

T

N

L

F

I

F

S

S

Y

F

L

F

T

N

G

W

P

L

V

C

T

V

V

A

M

L

T

A

-

I

-

G

-

M

-

I

-

W

L

L

R

R

K

H

T

R

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

G

F

F

F

V

I

F

L

A

A

S

C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T

C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

F

-

G

-

V

-

W

Y

W

Q

K

A

N

K

K

A

P

K

K

W

W

binding leucine: T19 (≠ S20), I20 (= I21), G22 (= G23), S23 (= S24), G212 (≠ N217)

8idaB Overall structure of the lat1-4f2hc bound with tyrosine
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/464 of 8idaB

query
sites
8idaB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S

T

I
|
I

I

I

G
|
G

S

S

G
|
G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V

I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M

I

S

V

S

A

W

L

P

V

W

F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W

Y

T

S

V

V

G

-

L

-

F

-

S

-

K

K

A

A

N

A

T

T

F

R

I

V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F

F

A

A

F

Y

N
x
G

G

G

F

W

Q

N

S
x
Y

P

L

I

N

N

F

M

V

A

T

G

E

E

E

A

M

K

I

N

N

P

P

G

Y

R

R

S

N

I

L

P

P

I

L

A

A

I

I

V

I

G

I

S

S

I

L

L

P

I

I

A

V

T

T

V

L

I

V

Y

Y

V

V

L

L

L

T

Q

N

I

L

A

A

F

Y

I

F

G

T

A

T

V

L

D

S

P

T

S

E

M

Q

I

M

V

L

N

S

G

S

W

E

G

A

-

V

H

A

L

V

N

D

F

F

N

G

S

N

P

-

F

-

A

-

D

-

L

-

A

-

I

Y

A

H

L

L

G

G

I

V

-

M

N

S

W

W

L

I

V

I

L

P

I

V

L

F

Y

V

A

G

-

L

D

S

A

C

F

F

V

G

S

S

P

V

S

N

G

G

T

S

G

-

I

-

T

-

Y

-

T

L

A

F

T

T

T

S

S

S

R

R

M

L

I

F

Y

F

G

V

M

G

E

S

K

R

N

E

K

G

Y

H

M

L

P

P

S

S

V

I

L

L

G

S

R

M

L

I

H

H

P

P

I

Q

Y

L

G

L

V

T

P

P

R

V

P

P

A

S

M

L

F

V

F

F

N

T

L

C

G

V

V

M

S

T

F

L

I

L

F

Y

L

A

F

F

L

-

F

-

R

-

G

-

W

-

G

-

V

-

L

S

A

K

E

D

I

I

I

F

S

S

V

V

A

I

T

N

L

F

I

F

S

S

Y

F

L

F

T

N

G

W

P

L

V

C

T

V

V

A

M

L

T

A

-

I

-

G

-

M

-

I

-

W

L

L

R

R

K

H

T

R

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

G

F

F

F

V

I

F

L

A

A

S

C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T

C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

F

-

G

-

V

-

W

Y

W

Q

K

A

N

K

K

A

P

K

K

W

W

binding tyrosine: I20 (= I21), G22 (= G23), G24 (= G25), G212 (≠ N217), Y216 (≠ S221)

7dsqB Overall structure of the lat1-4f2hc bound with 3,5-diiodo-l-tyrosine (see paper)
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/464 of 7dsqB

query
sites
7dsqB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S
x
T

I
|
I

I

I

G
|
G

S

S

G

G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V

I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M

I

S

V

S

A

W

L

P

V

W

F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W

Y

T

S

V

V

G

-

L

-

F

-

S

-

K
|
K

A

A

N

A

T

T

F

R

I
x
V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F

F

A

A

F

Y

N
x
G

G
|
G

F

W

Q

N

S
x
Y

P

L

I

N

N

F

M

V

A

T

G

E

E

E

A

M

K

I

N

N

P

P

G

Y

R

R

S

N

I

L

P

P

I

L

A

A

I

I

V

I

G

I

S

S

I

L

L

P

I

I

A

V

T

T

V

L

I

V

Y

Y

V

V

L

L

L

T

Q

N

I

L

A

A

F

Y

I

F

G

T

A

T

V

L

D

S

P

T

S

E

M

Q

I

M

V

L

N

S

G

S

W

E

G

A

-

V

H

A

L

V

N

D

F

F

N

G

S

N

P

-

F

-

A

-

D

-

L

-

A

-

I

Y

A

H

L

L

G

G

I

V

-

M

N

S

W

W

L

I

V

I

L

P

I

V

L

F

Y

V

A

G

-

L

D

S

A

C

F

F

V

G

S

S

P

V

S

N

G

G

T

S

G

-

I

-

T

-

Y

-

T

L

A

F

T

T

T

S

S

S

R

R

M

L

I

F

Y

F

G

V

M

G

E

S

K

R

N

E

K

G

Y

H

M

L

P

P

S

S

V

I

L

L

G

S

R

M

L

I

H

H

P

P

I

Q

Y

L

G

L

V

T

P

P

R

V

P

P

A

S

M

L

F

V

F

F

N

T

L

C

G

V

V

M

S

T

F

L

I

L

F

Y

L

A

F

F

L

-

F

-

R

-

G

-

W

-

G

-

V

-

L

S

A

K

E

D

I

I

I

F

S

S

V

V

A

I

T

N

L

F

I
x
F

S

S

Y

F

L

F

T
x
N

G

W

P

L

V

C

T

V

V

A

M

L

T

A

-

I

-

G

-

M

-

I

-

W

L

L

R

R

K

H

T

R

G

K

T

P

D

E

L

L

Y

E

R

R

P

P

L

I

R

K

I

V

K

-

G

-

L

-

S

N

I

L

I

A

A

L

P

P

L

V

G

F

F

F

V

I

F

L

A

A

S

C

L

L

T

F

L

L

-

I

-

A

-

V

-

S

Y

F

W

W

A

K

R

T

W

P

P

V

L

E

T

C

G

G

Q

I

V

G

L

F

F

T

I

I

L

L

I

S

G

G

L

L

P

P

I

V

Y

Y

F

F

Y

F

-

G

-

V

-

W

Y

W

Q

K

A

N

K

K

A

P

K

K

W

W

binding 3,5-diiodotyrosine: T19 (≠ S20), I20 (= I21), G22 (= G23), G212 (≠ N217), G213 (= G218), Y216 (≠ S221), F357 (≠ I376), N361 (≠ T380)
binding cholesterol hemisuccinate: K148 (= K159), V153 (≠ I164)

7dsnB Overall structure of the lat1-4f2hc bound with jx-119 (see paper)
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/464 of 7dsnB

query
sites
7dsnB

L

L

H

Q

R

R

R

N

M

I

G

T

T

L

F

L

A

N

L

G

M

V

M

A

V

I

G

I

L

V

G

G

S
x
T

I
|
I

I

I

G
|
G

S
|
S

G
|
G

W

I

L

F

F

V

G

T

A

P

W

T

R

G

A

V

A

L

Q

K

I

E

A

A

G

G

P

S

A

P

-

G

-

L

A

A

I

L

L

V

S

V

W

W

L

A

I

A

G

C

M

G

V

V

V

F

I

S

L

I

F

V

I

G

A

A

L

L

S

C

Y

Y

S

A

E

E

L

L

G

G

S

T

M

T

F

I

P

S

E

K

A

S

G

G

M

D

V

Y

K

A

Y

Y

T

M

Q

L

Y

E

S

V

H

Y

G

G

S

S

F

L

I

P

G

A

F

F

I

L

A

K

A

L

W

-

A

-

N

-

W

W

I

I

A

E

I

L

V

L

S

I

V
x
I

I

R

P

P

V

-

E

-

A

-

V

-

A

-

S

S

V

S

Q

Q

Y

Y

M
x
I

S

V

S

A

W

L

P

V

W
x
F

E

A

W

T

A

Y

K

L

W

L

T

K

S

P

H

L

L

F

V

P

T

T

K

C

G

P

V

V

L

P

T

E

E

A

G

A

L

K

M

L

V

V

A

A

S

C

V

L

L

C

L

V

V

L

I

L

Y

L

F

T

L

A

L

V

N

N

Y

C

W
x
Y

T

S

V

V

G

-

L

-

F

-

S

-

K
|
K

A

A

N

A

T

T

F

R

I
x
V

T

Q

I

D

F

A

K

F

I

A

V

A

I

A

P

K

G

L

L

L

T

A

I

L

G

A

A

L

L

I

F

L

V

L

G

G

F

F

H

V

S

Q

E

I

N

G

F

K

T

G

S

D

G

V

T

S

S

N

I

L

A

D

P

P

N

N

G

-

W

F

A

S

S

F

V

E

L

G

T

T

A

K

V

L

A

D

T

V

S

G

G

N

I

I

V

V

-

L

-

A

-

L

-

Y

-

S

-

G

-

L

F
|
F

A
|
A

F

Y

N
x
G

G

G

F

W

Q

N

S

Y

P

L

I

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binding (2~{S})-2-azanyl-7-[[2-(1,3-benzoxazol-2-yl)phenyl]methoxy]-3,4-dihydro-1~{H}-naphthalene-2-carboxylic acid: T19 (≠ S20), I20 (= I21), G22 (= G23), S23 (= S24), G24 (= G25), I97 (≠ V99), I104 (≠ M111), F209 (= F214), A210 (= A215), G212 (≠ N217), I354 (≠ A373), N361 (≠ T380)
binding cholesterol hemisuccinate: F109 (≠ W116), Y145 (≠ W152), K148 (= K159), V153 (≠ I164), Q326 (≠ I338)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 457

7dslB Overall structure of the lat1-4f2hc bound with jx-078 (see paper)
24% identity, 83% coverage: 4:450/539 of query aligns to 3:441/464 of 7dslB

query
sites
7dslB

L

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binding (2~{S})-2-azanyl-7-[(2-phenylphenyl)methoxy]-3,4-dihydro-1~{H}-naphthalene-2-carboxylic acid: T19 (≠ S20), S23 (= S24), G24 (= G25), F209 (= F214), G212 (≠ N217), Y216 (≠ S221), I354 (≠ A373)

Query Sequence

>WP_035703024.1 NCBI__GCF_000691145.1:WP_035703024.1
MNHLHRRMGTFALMMVGLGSIIGSGWLFGAWRAAQIAGPAAILSWLIGMVVILFIALSYS
ELGSMFPEAGGMVKYTQYSHGSFIGFIAAWANWIAIVSVIPVEAVASVQYMSSWPWEWAK
WTSHLVTKGVLTTEGLMVASVLLVIYFLLNYWTVGLFSKANTFITIFKIVIPGLTIGALL
FVGFHSENFTSGTSIAPNGWASVLTAVATSGIVFAFNGFQSPINMAGEAKNPGRSIPIAI
VGSILIATVIYVLLQIAFIGAVDPSMIVNGWGHLNFNSPFADLAIALGINWLVLILYADA
FVSPSGTGITYTATTSRMIYGMEKNKYMPSVLGRLHPIYGVPRPAMFFNLGVSFIFLFLF
RGWGVLAEIISVATLISYLTGPVTVMTLRKTGTDLYRPLRIKGLSIIAPLGFVFASLTLY
WARWPLTGQVLFIILIGLPIYFYYQAKAKWKGFGRHFKAGAWMVVYLLVMMTISCLGSDK
FGGYNVIHYGWDMALIAVVALLFYMWALKSGFETEFLEDAKKINDELRAFQKEAASSKE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory