SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035703129.1 NCBI__GCF_000691145.1:WP_035703129.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HAC7 Succinyl-CoA:glutarate CoA-transferase; Dermal papilla-derived protein 13; Dicarboxyl-CoA:dicarboxylic acid coenzyme A transferase SUGCT; Succinate--hydroxymethylglutarate CoA-transferase; EC 2.8.3.-; EC 2.8.3.13 from Homo sapiens (Human) (see 3 papers)
33% identity, 100% coverage: 1:400/400 of query aligns to 35:432/438 of Q9HAC7

query
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Q9HAC7

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D205 (= D170) mutation to A: Loss of CoA transferase activity toward glutaryl-CoA and 3-hydroxy-3-methylglutarate substrates.
R329 (≠ W297) to W: in GA3; severely decreased protein stability and processing; dbSNP:rs137852860

9br7C Succinate--hydroxymethylglutarate CoA-transferase (see paper)
33% identity, 100% coverage: 2:400/400 of query aligns to 1:397/403 of 9br7C

query
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9br7C

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binding losartan carboxylic acid: V17 (≠ M18), L18 (≠ I19), W49 (≠ V50), Y60 (≠ R60), Y140 (≠ F140), D141 (≠ G141), V166 (≠ F166), D170 (= D170)

3ubmB Formyl-coa:oxalate coa-transferase from acetobacter aceti (see paper)
28% identity, 99% coverage: 6:399/400 of query aligns to 4:427/430 of 3ubmB

query
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3ubmB

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active site: Q17 (≠ I19), E140 (≠ G141), D182 (= D170), G261 (vs. gap), G262 (vs. gap)
binding coenzyme a: V16 (≠ M18), R38 (≠ I40), L72 (= L73), N73 (≠ D74), T74 (≠ L75), K75 (≠ H76), N96 (= N97), F97 (= F98), R98 (= R99), A101 (≠ T102), R104 (≠ K105), K125 (≠ S126), D182 (= D170), M213 (≠ L203)

P69902 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; Formyl-CoA transferase; EC 2.8.3.16 from Escherichia coli (strain K12) (see paper)
27% identity, 99% coverage: 4:400/400 of query aligns to 2:416/416 of P69902

query
sites
P69902

N

S

T

T

P

P

L

L

E

Q

G

G

I

I

K

K

I

V

L

L

D

D

V

F

S

T

T

G

M

V

I

Q

A

S

A

G

P

P

Y

S

G

C

A

T

V

Q

L

M

L

L

G

A

D

W

F

F

G

G

A

A

E

D

V

V

I

I

K

K

V

I

E

E

I
x
R

P

P

G

G

K

V

G

G

D

D

T

V

L

T

R

R

H

H

-

Q

V

L

G

R

P

D

F

I

A

P

D

D

G

I

E

D

P

A

L

L

R

Y

W

F

S

T

G

M

L

L

S

N

R

S

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

N

L

T

H

K

K

T

E

A

E

E

A

G

K

K

D

E

I

V

F

M

K

E

K

K

L

L

A

I

A

R

Q

E

V

A

D

D

V

I

I

L

I

V

E

E

N

N

F

F

R

H

P

P

G

G

T

A

L

I

E

D

K

H

W

M

D

G

V

F

G

T

Y

W

D

E

V

H

L

I

K

Q

E

E

I

I

N

N

P

P

R

R

L

L

I

I

M

F

I

G

R

S

V

I

S

K

G

G

Y

F

G

D

Q

E

T

C

G

S

P

P

F

Y

K

V

D

N

K

V

A

K

G

A

F

Y

G

E

T

N

P

V

A

A

T

Q

A

A

F

A

S

G

G

G

F

A

T

A

Y

S

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

L

S

V

P

S

S

A

F

A

S

A

L

L

T

G

D

D

Y

S

I

N

A

T

G

G

I

M

Y

H

V

L

A

L

F

I

A

G

T

L

V

L

T

A

A

A

M

L

Y

L

Y

H

R

R

D

E

T

-

H

-

S

K

E

T

G

G

T

R

G

G

Q

Q

M

R

V

V

D

T

L

M

A

S

L

M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

C

E

R

F

V

L

K

V

L

A

R

E

D

-

Q

-

R

Y

L

D

D

K

K

L

L

G

G

K

Y

V

L

R

E

E

E

-

Y

-

P

R

Q

S

Y

P

P

-

N

G

G

L

T

S

F

G

G

H

D

S

A

S

V

P

P

-

R

-

G

-

N

-

A

-

G

-

Q

-

P

-

G

-

W

-

I

-

L

-

K

A

C

G

K

T

G

Y

W

E

E

T

T

K

D

D

P

G

N

H

A

Y

Y

L

I

V

Y

L

F

V

-

T

T

S

I

T

Q

D

E

S

Q

T

N

F

W

N

E

R

N

L

T

A

C

E

K

A

A

M

I

N

G

R

K

L

P

D

E

L

W

L

I

E

T

N

D

E

P

K

A

F

Y

S

S

V

T

N

A

A

H

A

A

R

R

L

Q

K

P

H

H

N

I

D

F

E

D

M

I

D

F

A

A

I

E

V

I

S

E

Q

K

W

Y

I

T

A

V

S

T

K

I

T

D

R

K

D

H

D

E

V

A

L

V

D

A

I

Y

L

L

D

T

T

Q

H

F

G

D

V

I

P

P

V

C

S

A

P

P

I

V

L

L

S

S

I

M

R

K

D

E

I

I

F

S

E

L

H

D

P

P

Q

S

F

L

K

R

A

Q

R

S

E

G

N

S

I

V

V

V

E

E

V

V

H

E

H

Q

P

P

R

L

L

R

G

G

K

K

V

Y

K

L

V

T

P

V

G

G

I

C

I

P

P

M

K

K

F

F

E

S

K

A

T

F

P

T

G

P

R

D

I

I

R

K

H

-

I

A

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

A

E

A

I

V

L

L

T

Q

K

E

M

-

L

L

G

G

F

Y

T

S

E

D

E

E

C

I

K

A

Q

A

L

M

E

K

E

Q

K

N

G

H

V

A

I

I

R38 (≠ I40) binding CoA

1pt5A Crystal structure of gene yfdw of e. Coli (see paper)
27% identity, 99% coverage: 4:400/400 of query aligns to 1:415/415 of 1pt5A

query
sites
1pt5A

N

S

T

T

P

P

L

L

E

Q

G

G

I

I

K

K

I

V

L

L

D

D

V

F

S

T

T

G

M
x
V

I
x
Q

A
x
S

A

G

P

P

Y

S

G

C

A

T

V

Q

L

M

L

L

G

A

D

W

F

F

G

G

A

A

E

D

V

V

I

I

K

K

V

I

E

E

I
x
R

P

P

G

G

K

V

G

G

D

D

T

V

L

T

R

R

H

H

-

Q

V

L

G

R

P

D

F

I

A

P

D

D

G

I

E

D

P

A

L

L

R

Y

W

F

S

T

G

M

L

L

S

N

R

S

N

N

K

K

K

R

S

S

L

I

T

E

L
|
L

D
x
N

L
x
T

H
x
K

K

T

E

A

E

E

A

G

K

K

D

E

I

V

F

M

K

E

K

K

L

L

A

I

A

R

Q

E

V

A

D

D

V

I

I

L

I

V

E

E

N
|
N

F
|
F

R
x
H

P

P

G

G

T

A

L

I

E

D

K

H

W

M

D

G

V

F

G

T

Y

W

D

E

V

H

L

I

K

Q

E

E

I

I

N

N

P

P

R

R

L

L

I

I

M

F

I

G

R

S

V

I

S
x
K

G

G

Y

F

G

D

Q

E

T

C

G

S

P

P

F

Y

K

V

D

N

K

V

A
x
K

G
x
A

F
x
Y

G
x
E

T

N

P

V

A

A

T

Q

A

A

F

A

S

G

G

G

F

A

T

A

Y

S

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

L

S

V

P

S

S

A

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

T

G

G

I

M

Y

H

V

L

A

L

F

I

A

G

T

L

V

L

T

A

A

A

M

L

Y

L

Y

H

R

R

D

E

T

-

H

-

S

K

E

T

G

G

T

R

G

G

Q

Q

M

R

V

V

D

T

L

M

A

S

L
x
M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

C

E

R

F

V

L

K

V

L

A

R

E

D

-

Q

-

R

Y

L

D

D

K

K

L

L

G

G

K

Y

V

L

R

E

E

E

-

Y

-

P

R

Q

S

Y

P

P

-

N

G

G

L

T

S

F

G

G

H

D

S

A

S

V

P

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

I

-

L

-

K

A

C

G

K

T

G

Y

W

E

E

T

T

K

D

D

P

G

N

H

A

Y

Y

L

I

V

Y

L

F

V

-

T

T

S

I

T

Q

D

E

S

Q

T

N

F

W

N

E

R

N

L

T

A

C

E

K

A

A

M

I

N

G

R

K

L

P

D

E

L

W

L

I

E

T

N

D

E

P

K

A

F

Y

S

S

V

T

N

A

A

H

A

A

R

R

L

Q

K

P

H

H

N

I

D

F

E

D

M

I

D

F

A

A

I

E

V

I

S

E

Q

K

W

Y

I

T

A

V

S

T

K

I

T

D

R

K

D

H

D

E

V

A

L

V

D

A

I

Y

L

L

D

T

T

Q

H

F

G

D

V

I

P

P

V

C

S

A

P

P

I

V

L

L

S

S

I

M

R

K

D

E

I

I

F

S

E

L

H

D

P

P

Q

S

F

L

K

R

A

Q

R

S

E

G

N

S

I

V

V

V

E

E

V

V

H

E

H

Q

P

P

R

L

L

R

G

G

K

K

V

Y

K

L

V

T

P

V

G

G

I

C

I

P

P

M

K

K

F

F

E

S

K

A

T

F

P

T

G

P

R

D

I

I

R

K

H

-

I

A

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

A

E

A

I

V

L

L

T

Q

K

E

M

-

L

L

G

G

F

Y

T

S

E

D

E

E

C

I

K

A

Q

A

L

M

E

K

E

Q

K

N

G

H

V

A

I

I

active site: Q16 (≠ I19), E139 (≠ G141), D168 (= D170), G247 (vs. gap), G248 (vs. gap)
binding acetyl coenzyme *a: V15 (≠ M18), S17 (≠ A20), R37 (≠ I40), L71 (= L73), N72 (≠ D74), T73 (≠ L75), K74 (≠ H76), N95 (= N97), F96 (= F98), H97 (≠ R99), K124 (≠ S126), K136 (≠ A138), A137 (≠ G139), Y138 (≠ F140), E139 (≠ G141), D168 (= D170), M199 (≠ L203)

1q6yA Hypothetical protein yfdw from e. Coli bound to coenzyme a (see paper)
27% identity, 99% coverage: 4:400/400 of query aligns to 2:416/417 of 1q6yA

query
sites
1q6yA

N

S

T

T

P

P

L

L

E

Q

G

G

I

I

K

K

I

V

L

L

D

D

V

F

S

T

T

G

M
x
V

I
x
Q

A
x
S

A

G

P

P

Y

S

G

C

A

T

V

Q

L

M

L

L

G

A

D

W

F

F

G

G

A

A

E

D

V

V

I

I

K

K

V

I

E

E

I
x
R

P

P

G

G

K

V

G

G

D

D

T

V

L

T

R

R

H

H

-

Q

V

L

G

R

P

D

F

I

A

P

D

D

G

I

E

D

P

A

L

L

R

Y

W

F

S

T

G

M

L

L

S

N

R

S

N

N

K

K

K

R

S

S

L

I

T

E

L
|
L

D
x
N

L
x
T

H
x
K

K

T

E

A

E

E

A

G

K

K

D

E

I

V

F

M

K

E

K

K

L

L

A

I

A

R

Q

E

V

A

D

D

V

I

I

L

I

V

E

E

N
|
N

F
|
F

R
x
H

P

P

G

G

T

A

L

I

E

D

K

H

W
x
M

D

G

V

F

G

T

Y

W

D

E

V

H

L

I

K

Q

E

E

I

I

N

N

P

P

R

R

L

L

I

I

M

F

I

G

R

S

V
x
I

S

K

G

G

Y

F

G

D

Q

E

T

C

G

S

P

P

F

Y

K

V

D

N

K

V

A
x
K

G
x
A

F
x
Y

G
x
E

T

N

P

V

A

A

T

Q

A

A

F

A

S

G

G

G

F

A

T

A

Y

S

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

L

S

V

P

S

S

A

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

T

G

G

I

M

Y

H

V

L

A

L

F

I

A

G

T

L

V

L

T

A

A

A

M

L

Y

L

Y

H

R

R

D

E

T

-

H

-

S

K

E

T

G

G

T

R

G

G

Q

Q

M

R

V

V

D

T

L

M

A

S

L
x
M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

C

E

R

F

V

L

K

V

L

A

R

E

D

-

Q

-

R

Y

L

D

D

K

K

L

L

G

G

K

Y

V

L

R

E

E

E

-

Y

-

P

R

Q

S

Y

P

P

-

N

G

G

L

T

S

F

G

G

H

D

S

A

S

V

P

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

I

-

L

-

K

A

C

G

K

T

G

Y

W

E

E

T

T

K

D

D

P

G

N

H

A

Y

Y

L

I

V

Y

L

F

V

-

T

T

S

I

T

Q

D

E

S

Q

T

N

F

W

N

E

R

N

L

T

A

C

E

K

A

A

M

I

N

G

R

K

L

P

D

E

L

W

L

I

E

T

N

D

E

P

K

A

F

Y

S

S

V

T

N

A

A

H

A

A

R

R

L

Q

K

P

H

H

N

I

D

F

E

D

M

I

D

F

A

A

I

E

V

I

S

E

Q

K

W

Y

I

T

A

V

S

T

K

I

T

D

R

K

D

H

D

E

V

A

L

V

D

A

I

Y

L

L

D

T

T

Q

H

F

G

D

V

I

P

P

V

C

S

A

P

P

I

V

L

L

S

S

I

M

R

K

D

E

I

I

F

S

E

L

H

D

P

P

Q

S

F

L

K

R

A

Q

R

S

E

G

N

S

I

V

V

V

E

E

V

V

H

E

H

Q

P

P

R

L

L

R

G

G

K

K

V

Y

K

L

V

T

P

V

G

G

I

C

I

P

P

M

K

K

F

F

E

S

K

A

T

F

P

T

G

P

R

D

I

I

R

K

H

-

I

A

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

A

E

A

I

V

L

L

T

Q

K

E

M

-

L

L

G

G

F

Y

T

S

E

D

E

E

C

I

K

A

Q

A

L

M

E

K

E

Q

K

N

G

H

V

A

I

I

active site: Q17 (≠ I19), E140 (≠ G141), D169 (= D170), G248 (vs. gap), G249 (vs. gap)
binding coenzyme a: V16 (≠ M18), Q17 (≠ I19), S18 (≠ A20), R38 (≠ I40), L72 (= L73), N73 (≠ D74), T74 (≠ L75), K75 (≠ H76), N96 (= N97), F97 (= F98), H98 (≠ R99), M105 (≠ W106), I124 (≠ V125), K137 (≠ A138), A138 (≠ G139), Y139 (≠ F140), D169 (= D170), M200 (≠ L203)

1q7eA Crystal structure of yfdw protein from e. Coli (see paper)
27% identity, 99% coverage: 4:400/400 of query aligns to 2:409/410 of 1q7eA

query
sites
1q7eA

N

S

T

T

P

P

L

L

E

Q

G

G

I

I

K

K

I

V

L

L

D

D

V

F

S

T

T

G

M

V

I
x
Q

A

S

A

G

P

P

Y

S

G

C

A

T

V

Q

L

M

L

L

G

A

D

W

F

F

G

G

A

A

E

D

V

V

I

I

K

K

V

I

E

E

I

R

P

P

G

G

K

V

G

G

D

D

T

V

L

T

R

R

H

H

-

Q

V

L

G

R

P

D

F

I

A

P

D

D

G

I

E

D

P

A

L

L

R

Y

W

F

S

T

G

M

L

L

S

N

R

S

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

N

L

T

H

K

K

T

E

A

E

E

A

G

K

K

D

E

I

V

F

M

K

E

K

K

L

L

A

I

A

R

Q

E

V

A

D

D

V

I

I

L

I

V

E

E

N
|
N

F
|
F

R
x
H

P
|
P

G

F

T

T

L

W

E

E

K

H

W

-

D

-

V

-

G

-

Y

-

D

-

V

-

L

I

K

Q

E

E

I

I

N

N

P

P

R

R

L

L

I

I

M

F

I

G

R

S

V

I

S
x
K

G

G

Y

F

G

D

Q

E

T

C

G

S

P

P

F

Y

K

V

D

N

K

V

A
x
K

G
x
A

F

Y

G
x
E

T

N

P

V

A

A

T

Q

A

A

F

A

S

G

G

G

F

A

T

A

Y

S

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

L

S

V

P

S

S

A

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

T

G

G

I

M

Y

H

V

L

A

L

F

I

A

G

T

L

V

L

T

A

A

A

M

L

Y

L

Y

H

R

R

D

E

T

-

H

-

S

K

E

T

G

G

T

R

G

G

Q

Q

M

R

V

V

D

T

L

M

A

S

L

M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

C

E

R

F

V

L

K

V

L

A

R

E

D

-

Q

-

R

Y

L

D

D

K

K

L

L

G

G

K

Y

V

L

R

E

E

E

-

Y

-

P

R

Q

S

Y

P

P

-

N

G

G

L

T

S

F

G

G

H

D

S

A

S

V

P

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-
x
W

-

I

-

L

-

K

A

C

G

K

T

G

Y

W

E

E

T

T

K

D

D

P

G

N

H

A

Y

Y

L

I

V

Y

L

F

V

-

T

T

S

I

T

Q

D

E

S

Q

T

N

F

W

N

E

R

N

L

T

A

C

E

K

A

A

M

I

N

G

R

K

L

P

D

E

L

W

L

I

E

T

N

D

E

P

K

A

F

Y

S

S

V

T

N

A

A

H

A

A

R

R

L

Q

K

P

H

H

N

I

D
x
F

E

D

M

I

D

F

A
|
A

I

E

V

I

S
x
E

Q

K

W

Y

I

T

A

V

S

T

K

I

T

D

R

K

D

H

D

E

V

A

L

V

D

A

I

Y

L

L

D

T

T

Q

H

F

G

D

V

I

P

P

V

C

S

A

P

P

I

V

L

L

S

S

I

M

R

K

D

E

I

I

F

S

E

L

H

D

P

P

Q

S

F

L

K

R

A

Q

R

S

E

G

N

S

I

V

V

V

E

E

V

V

H

E

H

Q

P

P

R

L

L

R

G

G

K

K

V

Y

K

L

V

T

P

V

G

G

I

C

I

P

P

M

K

K

F

F

E

S

K

A

T

F

P

T

G

P

R

D

I

I

R

K

H

-

I

A

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

A

E

A

I

V

L

L

T

Q

K

E

M

-

L

L

G

G

F

Y

T

S

E

D

E

E

C

I

K

A

Q

A

L

M

E

K

E

Q

K

N

G

H

V

A

I

I

active site: Q17 (≠ I19), E133 (≠ G141), D162 (= D170), G241 (vs. gap), G242 (vs. gap)
binding methionine: N96 (= N97), F97 (= F98), H98 (≠ R99), P99 (= P100), K118 (≠ S126), K130 (≠ A138), A131 (≠ G139), W246 (vs. gap), F299 (≠ D288), A303 (= A292), E306 (≠ S295)

Q9UHK6 Alpha-methylacyl-CoA racemase; 2-methylacyl-CoA racemase; EC 5.1.99.4 from Homo sapiens (Human) (see 5 papers)
27% identity, 99% coverage: 7:400/400 of query aligns to 3:373/382 of Q9UHK6

query
sites
Q9UHK6

L

L

E

Q

G

G

I

I

K

S

I

V

L
x
V

D

E

V

L

S

S

T

G

M

L

I

A

A

P

A

G

P

P

Y

F

G

C

A

A

V

M

L

V

L

L

G

A

D

D

F

F

G

G

A

A

E

R

V

V

I

V

K

R

V

V

E

D

I

R

P

P

G

G

K

S

G

R

D

Y

T

D

L

V

R

S

H

R

V

L

G

G

P

-

F

-

A

-

D

-

G

-

E

-

P

-

L

-

R

-

W

-

S

-

G

-

L

-

S

-

R

R

N

G

K

K

K

R

S
|
S

L

L

T

V

L

L

D

D

L

L

H

K

K

Q

E

P

E

R

A

G

K

A

D

A

I

V

F

L

K

R

L

L

A

C

A

K

Q

R

V

S

D

D

V

V

I

L

E

E

N

P

F

F

R

R

P

R

G

G

T

V

L

M

E

E

K

K

W

L

D

Q

V

L

G

G

Y

P

D

E

V

I

L

L

K

Q

E

R

I

E

N

N

P

P

R

R

L

L

I

I

M

Y

I

A

R

R

V
x
L

S

S

G

G

Y

F

G

G

Q

Q

T

S

G

G

P

S

F

F

K

C

D

R

K

L

A

A

G

G

F

H

G

D

T

I

P

N

A

Y

T

L

A

A

F

L

S

S

G

G

F

V

T

L

Y

S

I

K

Q

I

G

G

F

R

T

S

D

G

R

E

H

N

P

P

V

Y

S

A

P

P

S

L

F

N

S

L

L

L

T

A

D

D

Y

F

I

A

-

G

A

G

G

G

I

L

Y

M

V

C

A

A

F

L

A

G

T

I

V

I

T

M

A

A

M

L

Y

F

Y

-

R

-

D

D

T

R

H

T

S

R

E

T

G
|
G

T

K

G

G

Q

Q

M

V

V

I

D

D

L

A

A

N

L

M

Y

V

E

E

S

G

V

T

F

A

R

Y

M

L

L

S

E

S

F

F

L

L

V

W

-

K

A

T

E

Q

Y

K

D
x
L

K

S

L

L

G

W

K

E

V

A

R

P

E

R

R

G

S

Q

P

N

G

M

L

L

S

D

G

G

H

G

S

A

S

P

P

F

A

Y

G

T

T

T

Y

Y

E

R

T

T

K

A

D

D

G

G

H

E

Y

F

L

M

V

A

L

V

V

G

T

A

S

I

T

E

D

P

S

Q

T

F

F

Y

N

E

R

L

L

L

A

I

E

K

A

G

M

L

N

G

R

-

L

-

D

-

L

-

L

L

E

K

N

S

E

D

K

E

F

L

S

P

V

-

N

N

A

Q

A

M

R

S

L
x
M

K

D

H

D

N

W

D

P

E

E

M

M

D

K

A

K

I

K

V

F

S

A

Q

D

W

V

I

F

A

A

S
x
E

K

K

T

T

R

K

D

A

D

E

V

W

L

C

D

Q

I

I

L

F

D

D

T

G

H

T

G

D

V

A

P

C

V

V

S

T

P

P

I

V

L

L

S

T

I

F

R

E

D

E

I

V

F

V

E

H

H

H

P

D

Q

H

F

N

K

K

A

E

R

R

E

G

N

S

I

F

V

I

E

T

V

S

H

E

P

Q

R

D

L

V

G

S

K

P

V

R

K

P

V

A

P

P

G

L

I

L

-

L

-

N

-

T

-

P

-

A

I

I

P

P

K

S

F

F

E

K

K

R

T

D

P

P

G

F

R

-

I

-

R

-

H

-

I

-

A

-

P

-

D

-

L

I

G

G

E

E

H

H

N

T

Y

E

E

E

I

I

L

L

T

E

K

E

M

-

L

F

G

G

F

F

T

S

E

R

E

E

C

I

K

Y

Q

Q

L

L

E

N

E

S

K

D

G

K

V

I

I

I

V9 (≠ L13) to M: in dbSNP:rs3195676
S52 (= S70) to P: in AMACRD and CBAS4; inactive enzyme; dbSNP:rs121917814
L107 (≠ V125) to P: in CBAS4; inactive enzyme; dbSNP:rs121917816
G175 (= G194) to D: in dbSNP:rs10941112
L201 (≠ D219) to S: in dbSNP:rs2287939
M261 (≠ L284) to T: in dbSNP:rs3195678
E277 (≠ S300) to K: in dbSNP:rs2278008

Sites not aligning to the query:

380:382 Microbody targeting signal

1xvtA Crystal structure of native caib in complex with coenzyme a (see paper)
28% identity, 100% coverage: 1:399/400 of query aligns to 3:397/402 of 1xvtA

query
sites
1xvtA

M

M

N

P

K

K

N

F

T

G

P

P

L

L

E

A

G

G

I

L

K

R

I

V

L

V

D

F

V

S

S

G

T

I

M

E

I
|
I

A
|
A

A

G

P

P

Y

F

G

A

A

G

V

Q

L

M

L

F

G

A

D

E

F

W

G

G

A

A

E

E

V

V

I

I

K

W

V

I

E

E

I
x
N

P

V

G

A

K

W

G

A

D

D

T

T

L

I

R

R

H

-

V

V

G

Q

P

P

F

-

A

-

D

-

G

-

E

-

P

-

L

-

R

N

W

Y

S

P

G

Q

L

L

S

S

R

R

-

R

N

N

K

L

K

H

S

A

L

L

T

S

L
|
L

D
x
N

L

I

H
x
F

K

K

E

D

E

E

A

G

K

R

D

E

I

A

F

F

K

L

K

K

L

L

A

M

A

E

Q

T

V

T

D

D

V

I

I

F

I

I

E

E

N

A

F
x
S

R
x
K

P

G

G

P

T

A

L

F

E

A

K
x
R

W
x
R

D

G

V

I

G

T

Y

D

D

E

V

V

L

L

K

W

E

Q

I

H

N

N

P

P

R

K

L

L

I

V

M

I

I

A

R

H

V

L

S

S

G

G

Y

F

G

G

Q

Q

T

Y

G

G

P

T

-

E

F

Y

K

T

D

N

K

L

A
x
P

G
x
A

F

Y

G
x
N

T

T

P

I

A

A

T

Q

A

A

F

F

S

S

G

G

F

Y

T

L

Y

I

I

Q

Q

N

G

G

F

D

T

V

D

D

R

Q

H

-

P

P

V

M

S

-

P

P

S

A

F

F

S

P

L

Y

T

T

-

A

D
|
D

Y

Y

I

F

A

S

G

G

I

L

Y

T

V

A

A

T

F

T

A

A

T

A

V

L

T

A

A

A

M

L

Y

H

-

K

Y

V

R

R

D

E

T

T

H

-

S

-

E

-

G

G

T

K

G

G

Q

E

M

S

V

I

D

D

L

I

A

A

L
x
M

Y

Y

E

E

S

V

V

M

F

L

R

R

M

M

L

G

E

Q

F

Y

L

F

V

M

A

M

E

D

Y

Y

D

F

K

N

L

G

G

G

K

E

V

M

R

C

E

P

R

R

-

M

S

S

P

K

G
|
G

L
x
K

S

D

G

P

H

Y

S

Y

S

A

P

G

A

C

G

G

T

L

Y

Y

E

K

T

C

K

A

D

D

G

G

H

Y

Y

I

-

V

-

M

-

E

L

L

V

V

L

G

V

I

T

T

S

Q

T

I

D

E

S

E

T

C

F

F

N

K

R

D

-

I

-

G

L

L

A

A

E

H

A

L

M

L

N

G

R

T

L

P

D

E

L

I

L

P

E

E

N

G

E

T

K

Q

F

L

S

-

V

I

N

H

A

R

A

I

R

E

L

C

K

P

H

Y

N

G

D

P

E

L

M

V

D

E

A

E

I

K

V

L

S

D

Q

A

W

W

I

L

A

A

S

T

K

H

T

T

R

I

D

A

D

E

V

V

L

K

D

E

I

R

L

F

D

A

T

E

H

L

G

N

V

I

P

A

V

C

S

A

P

K

I

V

L

L

S

T

I

V

R

P

D

E

I

L

F

E

E

S

H

N

P

P

Q

Q

F

Y

K

V

A

A

R

R

E

E

N

S

I

I

V

T

E

Q

V

W

H

Q

H

T

P

M

R

D

L

G

G

R

K

T

V

C

K

K

V

G

P

P

G

N

I

I

I

M

P

P

K

K

F

F

E

K

K

N

T

N

P

P

G

G

R

Q

I

I

R

W

H

R

I

G

A

M

P

P

D

S

L

H

G

G

E

M

H

D

N

T

Y

A

E

A

I

I

L

L

T

-

K

K

M

N

L

I

G

G

F

Y

T

S

E

E

E

N

E

D

C

I

K

Q

Q

E

L

L

E

V

E

S

K

K

G

G

V

L

active site: I21 (= I19), N138 (≠ G141), D166 (= D170), G225 (= G230), K226 (≠ L231)
binding coenzyme a: I21 (= I19), A22 (= A20), N42 (≠ I40), L68 (= L73), N69 (≠ D74), F71 (≠ H76), S93 (≠ F98), K94 (≠ R99), R100 (≠ K105), R101 (≠ W106), P135 (≠ A138), A136 (≠ G139), D166 (= D170), M197 (≠ L203)

P31572 L-carnitine CoA-transferase; Crotonobetainyl-CoA:carnitine CoA-transferase; EC 2.8.3.21 from Escherichia coli (strain K12) (see paper)
28% identity, 100% coverage: 1:399/400 of query aligns to 6:400/405 of P31572

query
sites
P31572

M

M

N

P

K

K

N

F

T

G

P

P

L

L

E

A

G

G

I

L

K

R

I

V

L

V

D

F

V

S

S

G

T

I

M

E

I

I

A

A

A

G

P

P

Y

F

G

A

A

G

V

Q

L

M

L

F

G

A

D

E

F

W

G

G

A

A

E

E

V

V

I

I

K

W

V

I

E

E

I

N

P

V

G

A

K

W

G

A

D

D

T

T

L

I

R

R

H

-

V

V

G

Q

P

P

F

-

A

-

D

-

G

-

E

-

P

-

L

-

R

N

W

Y

S

P

G

Q

L

L

S

S

R

R

-

R

N

N

K

L

K

H

S

A

L

L

T

S

L

L

D

N

L

I

H

F

K

K

E

D

E

E

A

G

K

R

D

E

I

A

F

F

K

L

K

K

L

L

A

M

A

E

Q

T

V

T

D

D

V

I

I

F

I

I

E

E

N

A

F

S

R
x
K

P

G

G

P

T

A

L

F

E

A

K

R

W
x
R

D

G

V

I

G

T

Y

D

D

E

V

V

L

L

K

W

E

Q

I

H

N

N

P

P

R

K

L

L

I

V

M

I

I

A

R

H

V

L

S

S

G

G

Y

F

G

G

Q

Q

T

Y

G

G

P

T

-

E

F

Y

K

T

D

N

K

L

A

P

G

A

F

Y

G

N

T

T

P

I

A

A

T

Q

A

A

F

F

S

S

G

G

F

Y

T

L

Y

I

I

Q

Q

N

G

G

F

D

T

V

D

D

R

Q

H

-

P

P

V

M

S

-

P

P

S

A

F

F

S

P

L

Y

T

T

-

A

D

D

Y

Y

I

F

A

S

G

G

I

L

Y

T

V

A

A

T

F

T

A

A

T

A

V

L

T

A

A

A

M

L

Y

H

-

K

Y

V

R

R

D

E

T

T

H

-

S

-

E

-

G

G

T

K

G

G

Q

E

M

S

V

I

D

D

L

I

A

A

L

M

Y

Y

E

E

S

V

V

M

F

L

R

R

M

M

L

G

E

Q

F

Y

L

F

V

M

A

M

E

D

Y

Y

D

F

K

N

L

G

G

G

K

E

V

M

R

C

E

P

R

R

-

M

S

S

P

K

G

G

L

K

S

D

G

P

H

Y

S

Y

S

A

P

G

A

C

G

G

T

L

Y

Y

E

K

T

C

K

A

D

D

G

G

H

Y

Y

I

-

V

-

M

-

E

L

L

V

V

L

G

V

I

T

T

S

Q

T

I

D

E

S

E

T

C

F

F

N

K

R

D

-

I

-

G

L

L

A

A

E

H

A

L

M

L

N

G

R

T

L

P

D

E

L

I

L

P

E

E

N

G

E

T

K

Q

F

L

S

-

V

I

N

H

A

R

A

I

R

E

L

C

K

P

H

Y

N

G

D

P

E

L

M

V

D

E

A

E

I

K

V

L

S

D

Q

A

W

W

I

L

A

A

S

T

K

H

T

T

R

I

D

A

D

E

V

V

L

K

D

E

I

R

L

F

D

A

T

E

H

L

G

N

V

I

P

A

V

C

S

A

P

K

I

V

L

L

S

T

I

V

R

P

D

E

I

L

F

E

E

S

H

N

P

P

Q

Q

F

Y

K

V

A

A

R

R

E

E

N

S

I

I

V

T

E

Q

V

W

H

Q

H

T

P

M

R

D

L

G

G

R

K

T

V

C

K

K

V

G

P

P

G

N

I

I

I

M

P

P

K

K

F

F

E

K

K

N

T

N

P

P

G

G

R

Q

I

I

R

W

H

R

I

G

A

M

P

P

D

S

L

H

G

G

E

M

H

D

N

T

Y

A

E

A

I

I

L

L

T

-

K

K

M

N

L

I

G

G

F

Y

T

S

E

E

E

N

E

D

C

I

K

Q

Q

E

L

L

E

V

E

S

K

K

G

G

V

L

K97 (≠ R99) binding CoA
R104 (≠ W106) binding CoA

1xvvA Crystal structure of caib mutant d169a in complex with carnitinyl-coa (see paper)
28% identity, 100% coverage: 1:399/400 of query aligns to 3:397/402 of 1xvvA

query
sites
1xvvA

M

M

N

P

K

K

N

F

T

G

P

P

L

L

E

A

G

G

I

L

K

R

I

V

L

V

D

F

V

S

S

G

T
x
I

M
x
E

I
|
I

A
|
A

A

G

P

P

Y

F

G

A

A

G

V

Q

L

M

L

F

G

A

D

E

F

W

G

G

A

A

E

E

V

V

I

I

K

W

V

I

E

E

I

N

P

V

G

A

K

W

G

A

D

D

T

T

L

I

R

R

H

-

V

V

G

Q

P

P

F

-

A

-

D

-

G

-

E

-

P

-

L

-

R

N

W

Y

S

P

G

Q

L

L

S

S

R

R

-

R

N

N

K

L

K

H

S

A

L

L

T

S

L

L

D
x
N

L

I

H
x
F

K

K

E

D

E

E

A

G

K

R

D

E

I

A

F

F

K

L

K

K

L

L

A

M

A

E

Q

T

V

T

D

D

V

I

I

F

I

I

E

E

N
x
A

F
x
S

R
x
K

P

G

G

P

T

A

L

F

E

A

K
x
R

W
x
R

D

G

V

I

G

T

Y

D

D

E

V

V

L

L

K

W

E

Q

I

H

N

N

P

P

R

K

L

L

I

V

M

I

I

A

R

H

V

L

S

S

G

G

Y

F

G

G

Q

Q

T

Y

G

G

P

T

-

E

F

Y

K

T

D

N

K

L

A

P

G
x
A

F
x
Y

G
x
N

T

T

P

I

A

A

T

Q

A

A

F

F

S

S

G

G

F

Y

T

L

Y

I

I

Q

Q

N

G

G

F

D

T

V

D

D

R

Q

H

-

P

P

V

M

S

-

P

P

S

A

F

F

S

P

L
x
Y

T

T

-

A

D
x
A

Y

Y

I

F

A

S

G

G

I

L

Y

T

V

A

A

T

F

T

A

A

T

A

V

L

T

A

A

A

M

L

Y

H

-

K

Y

V

R

R

D

E

T

T

H

-

S

-

E

-

G

G

T

K

G

G

Q

E

M

S

V

I

D

D

L

I

A

A

L

M

Y

Y

E

E

S

V

V

M

F

L

R

R

M

M

L

G

E

Q

F

Y

L

F

V

M

A

M

E

D

Y

Y

D

F

K

N

L

G

G

G

K

E

V

M

R

C

E

P

R

R

-

M

S

S

P

K

G
|
G

L
x
K

S

D

G

P

H

Y

S

Y

S

A

P

G

A

C

G

G

T

L

Y

Y

E

K

T

C

K

A

D

D

G

G

H

Y

Y

I

-

V

-

M

-

E

L

L

V

V

L

G

V

I

T

T

S

Q

T

I

D

E

S

E

T

C

F

F

N

K

R

D

-

I

-

G

L

L

A

A

E

H

A

L

M

L

N

G

R

T

L

P

D

E

L

I

L

P

E

E

N

G

E

T

K

Q

F

L

S

-

V

I

N

H

A

R

A

I

R

E

L

C

K

P

H

Y

N

G

D

P

E

L

M

V

D

E

A

E

I

K

V

L

S

D

Q

A

W

W

I

L

A

A

S

T

K

H

T

T

R

I

D

A

D

E

V

V

L

K

D

E

I

R

L

F

D

A

T

E

H

L

G

N

V

I

P

A

V

C

S

A

P

K

I

V

L

L

S

T

I

V

R

P

D

E

I

L

F

E

E

S

H

N

P

P

Q

Q

F

Y

K

V

A

A

R

R

E

E

N

S

I

I

V

T

E

Q

V

W

H

Q

H

T

P

M

R

D

L

G

G

R

K

T

V

C

K

K

V

G

P

P

G

N

I

I

I

M

P

P

K

K

F

F

E

K

K

N

T

N

P

P

G

G

R

Q

I

I

R

W

H

R

I

G

A

M

P

P

D

S

L

H

G

G

E

M

H

D

N

T

Y

A

E

A

I

I

L

L

T

-

K

K

M

N

L

I

G

G

F

Y

T

S

E

E

E

N

E

D

C

I

K

Q

Q

E

L

L

E

V

E

S

K

K

G

G

V

L

active site: I21 (= I19), N138 (≠ G141), A166 (≠ D170), G225 (= G230), K226 (≠ L231)
binding l-carnitinyl-coa inner salt: I19 (≠ T17), E20 (≠ M18), I21 (= I19), A22 (= A20), N69 (≠ D74), F71 (≠ H76), A92 (≠ N97), S93 (≠ F98), K94 (≠ R99), R100 (≠ K105), R101 (≠ W106), A136 (≠ G139), Y137 (≠ F140), N138 (≠ G141), Y163 (≠ L168)

Q5U921 (R)-2-hydroxy-4-methylpentanoate CoA-transferase; 2-hydroxyisocaproate-CoA transferase; EC 2.8.3.24 from Clostridioides difficile (Peptoclostridium difficile) (see paper)
28% identity, 99% coverage: 7:400/400 of query aligns to 3:397/399 of Q5U921

query
sites
Q5U921

L

L

E

E

G

G

I

V

K

K

I

V

L

V

D

E

V

L

S

S

T

S

M

F

I

I

A

A

P

P

Y

C

G

C

A

A

V

K

L

M

L

L

G

G

D

D

F

W

G

G

A

A

E

E

V

V

I

I

K

K

V

I

E

E

I

-

P

P

G

I

K

E

G

G

D

D

T

G

L

I

R

R

H

V

V

M

G

G

-

G

-

T

-

F

-

K

-

S

P

P

F

A

A

S

D

D

G

D

E

E

P

N

L

P

R

M

W

F

S

E

G

L

L

E

S

N

R

G

N

N

K

K

S

G

L

V

T

S

L

I

D

N

L

V

H

K

K

S

E

K

E

E

A

G

K

V

D

E

I

I

F

L

K

H

K

K

L

L

A

L

A

S

Q

E

V

A

D

D

V

I

I

F

I

V

E

T

N

N

F

V

R

R

P

V

G

Q

T

A

L

L

E

E

K

K

W

M

D

G

V

I

G

A

Y

Y

D

D

V

Q

L

I

K

K

E

D

I

K

N

Y

P

P

R

G

L

L

I

I

M

F

I

S

R

Q

V

I

S

L

G

G

Y

Y

G

G

Q

E

T

K

G

G

P

P

F

L

K

K

D

D

K

K

A

P

G

G

F

F

G

D

-

Y

T

T

P

A

A

Y

T

F

A

A

F

R

S

G

G

G

F

V

T

S

Y

Q

I

S

Q

V

G

M

F

E

T

K

D

G

R

T

H

S

P

P

V

A

S

N

P

T

S

A

F

A

S

G

L

F

T

G

D
|
D

Y

H

I

Y

A

A

G

G

I

L

Y

A

V

L

A

A

F

A

A

G

T

S

V

L

T

A

A

A

M

L

Y

H

Y

K

R

K

D

-

T

-

H

A

S

Q

E

T

G

G

T

K

G

G

Q

E

M

R

V

V

D

T

L

V

A

S

L

L

Y

F

E

H

S

T

V

A

F

I

R

Y

M

G

L

M

E

G

F

T

L

M

V

I

-

T

A

A

E

Q

Y

Y

D

G

K

N

L

E

G

M

K

P

V

L

R

S

E

R

R

E

S

N

P

P

G

-

L

-

S

-

G

-

H

N

S

S

S

P

P

L

A

M

G

T

T

T

Y

Y

E

K

T

C

K

K

D

D

G

G

H

R

Y

W

L

I

V

Q

L

L

-

A

V

L

T

I

S

Q

T

Y

D

N

S

K

T

W

F

L

N

G

R

K

L

F

A

C

E

K

A

V

M

I

N

N

R

R

L

E

D

Y

L

I

L

L

E

E

N

D

E

D

K

R

F

Y

S

N

V

N

N

I

A

D

A

S

R

M

L

V

K

N

H

H

N

V

D

E

E

D

M

L

D

V

A

K

I

I

V

V

S

G

Q

E

W

A

I

M

A

L

S

E

K

K

T

T

R

L

D

D

D

E

V

W

L

S

D

A

I

L

L

L

D

E

T

E

H

A

G

D

V

L

P

P

V

F

S

E

P

K

I

I

L

Q

S

S

I

C

R

E

D

D

I

L

F

L

E

D

H

D

P

E

Q

Q

F

A

K

W

A

A

R

N

E

D

N

F

I

L

V

F

E

K

V

K

H

T

H

Y

P

D

R

S

L

-

G

G

K

N

V

T

K

G

V

V

P

L

G

V

I

N

I

T

P

P

K

V

F

M

E

F

K

R

T

N

P

E

G

G

R

I

I

K

R

E

H

Y

I

T

-

P

A

A

P

P

D

K

L

V

G

G

E

Q

H

H

N

T

Y

V

E

E

I

V

L

L

T

-

K

K

M

S

L

L

G

G

F

Y

T

D

E

E

E

K

C

I

K

N

Q

N

L

F

E

K

E

D

K

S

G

K

V

V

I

V

D171 (= D170) mutation D->A,N: Loss of activity.

5yx6A Crystal structure of rv3272 from m. Tuberculosis orthorhombic form (see paper)
29% identity, 95% coverage: 2:380/400 of query aligns to 1:360/360 of 5yx6A

query
sites
5yx6A

N

N

K

P

N

A

T

K

P

P

L

L

E

D

G

G

I

F

K

R

I

V

L

L

D

D

V

F

S

T

T

Q

M

N

I
x
V

A

A

A

G

P

P

Y

L

G

A

A

G

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Q

L

V

L

L

G

V

D

D

F

L

G

G

A

A

E

E

V

V

I

I

K

K

V

V

E

E

I

A

P

P

G

G

K

-

G

G

D

E

T

A

L

A

R

R

H

Q

V

I

G

T

P

S

F

V

A

L

D

P

G

G

E

R

P

P

L

P

R

L

W

A

S

T

G

Y

L

F

-

L

-

P

-

N

S

N

R

R

N

G

K

K

S

S

L

V

T

T

L

V

D

D

L

L

H

T

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T

E

E

E

Q

A

A

K

K

D

Q

I

Q

F

M

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L

K

R

L

L

A

A

A

D

Q

T

V

A

D

D

V

V

I

V

I

L

E

E

N
x
A

F

F

R

R

P

P

G

G

T

T

L

M

E

E

K

K

W

L

D

G

V

L

G

G

Y

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D

D

V

D

L

L

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N

N

P

P

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N

L

L

I

I

M

Y

I

A

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R

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L

S

T

G

A

Y

Y

G

G

Q

G

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N

G

G

P

P

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H

K

G

D

S

K

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A
x
P

G

G

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I

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x
D

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L

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A

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A

A

A

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A

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G

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M

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I

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G

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F

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T

P

D

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F

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D
|
D

Y

N

I

A

A

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G

G

I

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Y

V

V

L

A

A

F

Q

A

A

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V

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A

A

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Y

A

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x
T

S

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A

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N

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A

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D

L

L

L

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N

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F

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A

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A

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R

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N

N

Y

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A

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L

W

A

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K

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A

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T

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V

W

L

V

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I

L

L

L

D

Q

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A

H

N

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G

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M

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A

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C

P

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A

L

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S

T

I

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R

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D

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I

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F

V

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D

H

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P

P

Q

L

F

F

K

A

A

E

R

N

E

D

N

L

I

T

V

L

E

E

V

V

H

-

P

-

R

-

L

-

G

G

K

T

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I

K

T

V

V

P

I

G

R

I

T

I

P

P

A

K

R

F

Y

E

A

K

S

T

F

P

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G

A

R

V

I

V

R

T

H

D

I

P

A

P

P

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A

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G

E

E

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N

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Y

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L

L

active site: V18 (≠ I19), D142 (≠ G141), D171 (= D170), T221 (≠ S235)
binding glycerol: A98 (≠ N97), P139 (≠ A138)

O06543 Alpha-methylacyl-CoA racemase; AMACR; MtMCR; EC 5.1.99.4 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
27% identity, 89% coverage: 6:361/400 of query aligns to 4:337/360 of O06543

query
sites
O06543

P

P

L

L

E

S

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G

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L

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R

I

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L

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D

E

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A

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A

A

A

V

M

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I

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G

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D

F

L

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G

A

A

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D

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V

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I
x
R

P

P

G

S

K

S

G

V

D

D

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G

L

I

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S

H

R

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-

G

-

P

-

F

-

A

-

D

-

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-

E

-

P

-

L

-

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-

W

-

S

D

G

A

L

M

S

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R
|
R

N

N

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x
I

L

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T

T

L
x
A

D
|
D

L
|
L

H
x
K

K

S

E

D

E

Q

A

G

K

L

D

E

I

L

F

A

K

L

K

K

L

L

A

I

A

A

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K

V

A

D

D

V

V

I

L

I

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E
|
E

N
x
G

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x
Y

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|
R

P

P

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x
R

W

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G

V

L

G

G

Y

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D

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E

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C

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A

E

K

I

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N

N

P

D

R

R

L

L

I

I

M

Y

I

A

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x
M

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G

G

Y

W

G

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Q

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T

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G

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P

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S

D

Q

K

Q

A

A

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|
G

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x
H

G
x
D

T
x
I

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x
N

A
x
Y

T

I

A

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F

L

S

N

G

G

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I

A

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G

D

D

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R

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P

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S

P

P

P

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N

S

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D

Y

F

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A

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-

S

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Q

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D

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A

A

A

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Y

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x
D

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-

Y

A

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Y

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A

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D

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G

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R

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-

V

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L

A

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x
E

S

P

T

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F

F

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Y

R

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N

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L

D

D

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S

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-

N

D

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R

A

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W

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P

H

-

N

-

D

-

E

E

M

L

D

R

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A

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A

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R

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x
C

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P

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L

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D

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I

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H

E

N

H

E

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P

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x
H

F

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K

I

A

E

R

R

E

N

N

T

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F

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Y

E

E

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A

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P

-

R

-

L

-

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T

R38 (≠ I40) binding substrate
R52 (= R66) mutation to A: 15.7% of wild-type activity.
I56 (≠ S70) mutation to P: 28.8% of wild-type activity.
ADLK 59:62 (≠ LDLH 73:76) binding substrate
E82 (= E96) mutation to A: 12.5% of wild-type activity.
GYR 83:85 (≠ NFR 97:99) binding substrate
R91 (≠ K105) binding substrate; mutation to A: 19.9% of wild-type activity.
M111 (≠ V125) mutation to P: 5.2% of wild-type activity.
GHDINY 125:130 (≠ GFGTPA 139:144) binding substrate
H126 (≠ F140) mutation to A: 4.5% of wild-type activity.
D156 (= D170) mutation to A: 17.6 of wild-type activity.
D190 (≠ E205) mutation to A: 3.3% of wild-type activity.
E241 (≠ D256) mutation to A: 2.1% of wild-type activity.
C297 (≠ P316) mutation to A: 6.2% of wild-type activity.
H312 (≠ Q331) mutation to A: 10.1% of wild-type activity.

2vjoA Formyl-coa transferase mutant variant q17a with aspartyl-coa thioester intermediates and oxalate (see paper)
24% identity, 99% coverage: 6:400/400 of query aligns to 3:427/427 of 2vjoA

query
sites
2vjoA

P

P

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L

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D

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G

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x
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x
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A
|
A

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A

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C

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F

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G

G

A

A

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N

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K

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E

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x
R

P

R

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G

K

S

G

G

D

D

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M

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T

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R

H

-

V

-

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G

P

W

F

L

A

Q

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D

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K

-

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-

N

-

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L

R

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M

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C

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N

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K

K

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S

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E

L
|
L

D

D

L
x
M

H

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K

T

E

P

E

E

A

G

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K

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I

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M

A

I

A

K

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K

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A

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D

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E

N
|
N

F
|
F

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x
G

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G

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A

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x
R

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x
M

D

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V

S

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G

G

Y

Y

G

A

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T

G

G

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P

A

F

N

K

E

D

H

K

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A
x
K

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x
V

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x
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G
x
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A

A

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A

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A

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T

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A

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D
|
D

Y

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N

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Y

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-

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Q

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A

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A

A

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x
M

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A

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M

L

L

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F

I

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L

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Y

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D

Q

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-

K

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-

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E

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E

A

-

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D

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L

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N

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-

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x
G

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G

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x
Q

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A

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M

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N

D

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N

F

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R

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G

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N

V

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P

V

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G

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A

I

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F

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K

G

T

F

P

Q

G

P

R

E

I

I

R

T

H

R

I

-

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

D

E

E

I

V

L

L

T

-

K

K

M

E

L

L

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G

F

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D

E

D

E

A

E

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Q

E

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A

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Q

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V

active site: A16 (≠ I19), E139 (≠ G141), D168 (= D170), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ T17), A16 (≠ I19), A17 (= A20), R37 (≠ I40), L71 (= L73), M73 (≠ L75), N95 (= N97), F96 (= F98), G97 (≠ R99), R103 (≠ K105), M104 (≠ W106), K136 (≠ A138), V137 (≠ G139), Y138 (≠ F140), D168 (= D170), M199 (≠ L203)
binding oxalate ion: G257 (vs. gap), G259 (vs. gap), Q261 (vs. gap)

O06644 Formyl-CoA:oxalate CoA-transferase; FCOCT; Formyl-coenzyme A transferase; EC 2.8.3.16 from Oxalobacter formigenes (see 4 papers)
24% identity, 99% coverage: 6:400/400 of query aligns to 4:428/428 of O06644

query
sites
O06644

P

P

L

L

E

D

G

G

I

I

K

N

I

V

L

L

D

D

V

F

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T

T

H

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x
Q

A

A

A

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P

Y

A

G

C

A

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M

G

G

D

F

F

L

G

G

A

A

E

N

V

V

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I

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K

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I

E

E

I
x
R

P

R

G

G

K

S

G

G

D

D

T

M

L

T

R

R

H

-

V

-

G

G

P
x
W

F

L

A

Q

D

D

G

K

-

P

-

N

-

V

E

D

P

S

L

L

R

Y

W

F

S

T

G

M

L

F

S

N

R

C

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

D

L

M

H

K

K

T

E

P

E

E

A

G

K

K

D

E

I

L

F

L

K

E

K

Q

L

M

A

I

A

K

Q

K

V

A

D

D

V

V

I

M

I

V

E

E

N

N

F

F

R

G

P

P

G

G

T

A

L

L

E

D

K
x
R

W

M

D

G

V

F

G

T

Y

W

D

E

V

Y

L

I

K

Q

E

E

I

L

N

N

P

P

R

R

L

V

I

I

M

L

I

A

R

S

V

V

S

K

G

G

Y

Y

G

A

Q

E

T

G

G

H

P

A

F

N

K

E

D

H

K

L

A

K

G

V

F

Y

G

E

T

N

P

V

A

A

T

Q

A

C

F

S

S

G

G

G

F

A

T

A

Y

A

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

T

S

V

P

S

S

G

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

S

G

G

I

M

Y

H

V

L

A

M

F

I

A

G

T

I

V

L

T

A

A

A

M

L

Y

E

Y

M

R

R

D

-

T

-

H

H

S

K

E

T

G

G

T

R

G

G

Q

Q

M

K

V

V

D

A

L

V

A

A

L

M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

V

E

R

F

I

L

K

V

L

A

R

E

D

Y

Q

D

Q

K

R

L

L

G

-

K

-

V

-

R

-

E

E

R

R

S

T

P

G

G

I

L

L

S

A

G

E

H

Y

S

P

S

Q

P

A

A

Q

G

P

T

N

Y

F

E

A

-

F

T

D

K

R

D

D

G

G

H

N

Y

P

L

L

-

S

-

F

-

D

-

N

-

I

-

T

-

S

-

V

-

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

-

G

-

W

-

E

-

T

-

D

-

A

-

D

-

S

-

Y

V

V

L

Y

V

F

T

T

S

I

T

A

D

A

S

N

T

M

F

W

N

P

R

Q

L

I

A

C

E

D

A

M

M

I

N

D

R

K

L

P

D

E

L

W

L

K

E

D

N

D

E

P

K

A

F

Y

S

N

V

T

N

F

A

E

A

G

R

R

L

V

K

D

H

K

N

L

D

M

E

D

M

I

D

F

A

S

I

F

V

I

S

E

Q

T

W

K

I

F

A

A

S

D

K

K

T

D

R

K

D

F

D

E

V

V

L

T

D

E

I

W

L

A

D

A

T

Q

H

Y

G

G

V

I

P

P

V

C

S

G

P

P

I

V

L

M

S

S

I

M

R

K

D

E

I

L

F

A

E

H

H

D

P

P

Q

S

F

L

K

Q

A

K

R

V

E

G

N

T

I

V

V

V

E

E

V

V

H

V

H

D

P

E

R

I

L

R

G

G

K

N

V

H

K

L

V

T

P

V

G

G

I

A

I

P

P

F

K

K

F

F

E

S

K

G

T

F

P

Q

G

P

R

E

I

I

R

T

H

R

I

-

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

D

E

E

I

V

L

L

T

-

K

K

M

E

L

L

G

G

F

L

T

D

E

D

E

A

E

K

C

I

K

K

Q

E

L

L

E

H

E

A

K

K

G

Q

V

V

I

V

Q17 (≠ I19) mutation to A: 45-fold decrease of the catalytic effiency.
R38 (≠ I40) binding CoA
W48 (≠ P52) mutation to F: Little change in the affinity binding and catalytic efficiency, and it does not display major structural changes.; mutation to P: Little change in the affinity binding and catalytic efficiency. It exhibits substrate inhibition with oxalate. It does not display major structural changes.
R104 (≠ K105) binding CoA
D169 (= D170) active site, Nucleophile; mutation to A: Loss of CoA-transferase activity.; mutation to E: Loss of CoA-transferase activity.; mutation to S: Loss of CoA-transferase activity.
G259 (vs. gap) mutation to A: 2.5-fold decrease of the catalytic effiency.
G260 (vs. gap) mutation to A: 25-fold decrease of the catalytic effiency. Reduction of the affinity binding for both formyl-CoA and oxalate.

1p5rA Formyl-coa transferase in complex with coenzyme a (see paper)
24% identity, 99% coverage: 6:400/400 of query aligns to 3:427/427 of 1p5rA

query
sites
1p5rA

P

P

L

L

E

D

G

G

I

I

K

N

I

V

L

L

D

D

V

F

S

T

T
x
H

M
x
V

I
x
Q

A
|
A

A

G

P

P

Y

A

G

C

A

T

V

Q

L

M

G

G

D

F

F

L

G

G

A

A

E

N

V

V

I

I

K

K

V

I

E

E

I
x
R

P

R

G

G

K

S

G

G

D

D

T

M

L

T

R

R

H

-

V

-

G

G

P

W

F

L

A

Q

D

D

G

K

-

P

-

N

-

V

E

D

P

S

L

L

R

Y

W

F

S

T

G

M

L

F

S

N

R

C

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

D

L
x
M

H
x
K

K

T

E

P

E

E

A

G

K

K

D

E

I

L

F

L

K

E

K

Q

L

M

A

I

A

K

Q

K

V

A

D

D

V

V

I

M

I

V

E

E

N
|
N

F
|
F

R

G

P

P

G

G

T
x
A

L

L

E

D

K
x
R

W

M

D

G

V

F

G

T

Y

W

D

E

V

Y

L

I

K

Q

E

E

I

L

N

N

P

P

R

R

L

V

I

I

M

L

I

A

R

S

V

V

S

K

G

G

Y

Y

G

A

Q

E

T

G

G

H

P

A

F

N

K

E

D

H

K

L

A
x
K

G
x
V

F

Y

G
x
E

T

N

P

V

A

A

T

Q

A

C

F

S

S

G

G

G

F

A

T

A

Y

A

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

T

S

V

P

S

S

G

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

S

G

G

I

M

Y

H

V

L

A

M

F

I

A

G

T

I

V

L

T

A

A

A

M

L

Y

E

Y

M

R

R

D

-

T

-

H

H

S

K

E

T

G

G

T

R

G

G

Q

Q

M

K

V

V

D

A

L

V

A

A

L
x
M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

V

E

R

F

I

L

K

V

L

A

R

E

D

Y

Q

D

Q

K

R

L

L

G

-

K

-

V

-

R

-

E

E

R

R

S

T

P

G

G

I

L

L

S

A

G

E

H

Y

S

P

S

Q

P

A

A

Q

G

P

T

N

Y

F

E

A

-

F

T

D

K

R

D

D

G

G

H

N

Y

P

L

L

-

S

-

F

-

D

-

N

-

I

-

T

-

S

-

V

-

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

-

G

-

W

-

E

-

T

-

D

-

A

-

D

-

S

-

Y

V

V

L

Y

V

F

T

T

S

I

T

A

D

A

S

N

T

M

F

W

N

P

R

Q

L

I

A

C

E

D

A

M

M

I

N

D

R

K

L

P

D

E

L

W

L

K

E

D

N

D

E

P

K

A

F

Y

S

N

V

T

N

F

A

E

A

G

R

R

L

V

K

D

H

K

N

L

D

M

E

D

M

I

D

F

A

S

I

F

V

I

S

E

Q

T

W

K

I

F

A

A

S

D

K

K

T

D

R

K

D

F

D

E

V

V

L

T

D

E

I

W

L

A

D

A

T

Q

H

Y

G

G

V

I

P

P

V

C

S

G

P

P

I

V

L

M

S

S

I

M

R

K

D

E

I

L

F

A

E

H

H

D

P

P

Q

S

F

L

K

Q

A

K

R

V

E

G

N

T

I

V

V

V

E

E

V

V

H

V

H

D

P

E

R

I

L

R

G

G

K

N

V

H

K

L

V

T

P

V

G

G

I

A

I

P

P

F

K

K

F

F

E

S

K

G

T

F

P

Q

G

P

R

E

I

I

R

T

H

R

I

-

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

D

E

E

I

V

L

L

T

-

K

K

M

E

L

L

G

G

F

L

T

D

E

D

E

A

E

K

C

I

K

K

Q

E

L

L

E

H

E

A

K

K

G

Q

V

V

I

V

active site: Q16 (≠ I19), E139 (≠ G141), D168 (= D170), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ T17), V15 (≠ M18), Q16 (≠ I19), A17 (= A20), R37 (≠ I40), M73 (≠ L75), K74 (≠ H76), N95 (= N97), F96 (= F98), A100 (≠ T102), R103 (≠ K105), K136 (≠ A138), V137 (≠ G139), D168 (= D170), M199 (≠ L203)

2vjkA Formyl-coa transferase with aspartyl-coa thioester intermediate derived from oxalyl-coa (see paper)
24% identity, 99% coverage: 6:400/400 of query aligns to 3:427/427 of 2vjkA

query
sites
2vjkA

P

P

L

L

E

D

G

G

I

I

K

N

I

V

L

L

D

D

V

F

S

T

T
x
H

M

V

I
x
Q

A
|
A

A

G

P

P

Y

A

G

C

A

T

V

Q

L

M

G

G

D

F

F

L

G

G

A

A

E

N

V

V

I

I

K

K

V

I

E

E

I
x
R

P

R

G

G

K

S

G

G

D

D

T

M

L

T

R

R

H

-

V

-

G

G

P

W

F

L

A

Q

D

D

G

K

-

P

-

N

-

V

E

D

P

S

L

L

R

Y

W

F

S

T

G

M

L

F

S

N

R

C

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

D

L
x
M

H
x
K

K

T

E

P

E

E

A

G

K

K

D

E

I

L

F

L

K

E

K

Q

L

M

A

I

A

K

Q

K

V

A

D

D

V

V

I

M

I

V

E

E

N
|
N

F
|
F

R
x
G

P

P

G

G

T

A

L

L

E

D

K
x
R

W
x
M

D

G

V

F

G

T

Y

W

D

E

V

Y

L

I

K

Q

E

E

I

L

N

N

P

P

R

R

L

V

I

I

M

L

I

A

R

S

V

V

S

K

G

G

Y

Y

G

A

Q

E

T

G

G

H

P

A

F

N

K

E

D

H

K

L

A
x
K

G
x
V

F
x
Y

G
x
E

T

N

P

V

A

A

T

Q

A

C

F

S

S

G

G

G

F

A

T

A

Y

A

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

T

S

V

P

S

S

G

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

S

G

G

I

M

Y

H

V

L

A

M

F

I

A

G

T

I

V

L

T

A

A

A

M

L

Y

E

Y

I

R

R

D

-

T

-

H

H

S

K

E

T

G

G

T

R

G

G

Q

Q

M

K

V

V

D

A

L

V

A

A

L
x
M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

V

E

R

F

I

L

K

V

L

A

R

E

D

Y

Q

D

Q

K

R

L

L

G

-

K

-

V

-

R

-

E

E

R

R

S

T

P

G

G

I

L

L

S

A

G

E

H

Y

S

P

S

Q

P

A

A

Q

G

P

T

N

Y

F

E

A

-

F

T

D

K

R

D

D

G

G

H

N

Y

P

L

L

-

S

-

F

-

D

-

N

-

I

-

T

-

S

-

V

-

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

-

G

-

W

-

E

-

T

-

D

-

A

-

D

-

S

-

Y

V

V

L

Y

V

F

T

T

S

I

T

A

D

A

S

N

T

M

F

W

N

P

R

Q

L

I

A

C

E
x
D

A

M

M

I

N
x
D

R

K

L

P

D

E

L

W

L

K

E

D

N

D

E

P

K

A

F

Y

S

N

V

T

N

F

A

E

A

G

R

R

L

V

K

D

H

K

N

L

D

M

E

D

M

I

D

F

A

S

I

F

V

I

S

E

Q

T

W

K

I

F

A

A

S

D

K

K

T

D

R

K

D

F

D

E

V

V

L

T

D

E

I

W

L

A

D

A

T

Q

H

Y

G

G

V

I

P

P

V

C

S

G

P

P

I

V

L

M

S

S

I

M

R

K

D

E

I

L

F

A

E

H

H

D

P

P

Q

S

F

L

K

Q

A

K

R

V

E

G

N

T

I

V

V

V

E

E

V

V

H

V

H

D

P

E

R

I

L

R

G

G

K

N

V

H

K

L

V

T

P

V

G

G

I

A

I

P

P

F

K

K

F

F

E

S

K

G

T

F

P

Q

G

P

R

E

I

I

R

T

H

R

I

-

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

D

E

E

I

V

L

L

T

-

K

K

M

E

L

L

G

G

F

L

T

D

E

D

E

A

E

K

C

I

K

K

Q

E

L

L

E

H

E

A

K

K

G

Q

V

V

I

V

active site: Q16 (≠ I19), E139 (≠ G141), D168 (= D170), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ T17), Q16 (≠ I19), A17 (= A20), R37 (≠ I40), M73 (≠ L75), K74 (≠ H76), N95 (= N97), F96 (= F98), G97 (≠ R99), R103 (≠ K105), M104 (≠ W106), K136 (≠ A138), V137 (≠ G139), Y138 (≠ F140), D168 (= D170), M199 (≠ L203)
binding magnesium ion: D293 (≠ E264), D296 (≠ N267)

1t4cA Formyl-coa transferase in complex with oxalyl-coa (see paper)
24% identity, 99% coverage: 6:400/400 of query aligns to 3:427/427 of 1t4cA

query
sites
1t4cA

P

P

L

L

E

D

G

G

I

I

K

N

I

V

L

L

D

D

V

F

S

T

T
x
H

M
x
V

I
x
Q

A

A

A

G

P

P

Y

A

G

C

A

T

V

Q

L

M

G

G

D

F

F

L

G

G

A

A

E

N

V

V

I

I

K

K

V

I

E

E

I
x
R

P

R

G

G

K

S

G

G

D

D

T

M

L

T

R

R

H

-

V

-

G

G

P

W

F

L

A

Q

D

D

G

K

-

P

-

N

-

V

E

D

P

S

L

L

R

Y

W

F

S

T

G

M

L

F

S

N

R

C

N

N

K

K

K

R

S

S

L

I

T

E

L

L

D

D

L
x
M

H

K

K

T

E

P

E

E

A

G

K

K

D

E

I

L

F

L

K

E

K

Q

L

M

A

I

A

K

Q

K

V

A

D

D

V

V

I

M

I

V

E

E

N
|
N

F
|
F

R

G

P

P

G

G

T

A

L

L

E

D

K
x
R

W
x
M

D

G

V

F

G

T

Y

W

D

E

V

Y

L

I

K

Q

E

E

I

L

N

N

P

P

R

R

L

V

I

I

M

L

I

A

R

S

V

V

S

K

G

G

Y

Y

G

A

Q

E

T

G

G

H

P

A

F

N

K

E

D

H

K

L

A

K

G
x
V

F
x
Y

G
x
E

T

N

P

V

A

A

T

Q

A

C

F

S

S

G

G

G

F

A

T

A

Y

A

I

T

Q

T

G

G

F

F

T

W

D

D

R

G

H

P

P

P

V

T

S

V

P

S

S

G

F

A

S

A

L

L

T

G

D
|
D

Y

S

I

N

A

S

G

G

I

M

Y

H

V

L

A

M

F

I

A

G

T

I

V

L

T

A

A

A

M

L

Y

E

Y

I

R

R

D

-

T

-

H

H

S

K

E

T

G

G

T

R

G

G

Q

Q

M

K

V

V

D

A

L

V

A

A

L
x
M

Y

Q

E

D

S

A

V

V

F

L

R

N

M

L

L

V

E

R

F

I

L

K

V

L

A

R

E

D

Y

Q

D

Q

K

R

L

L

G

-

K

-

V

-

R

-

E

E

R

R

S

T

P

G

G

I

L

L

S

A

G

E

H

Y

S

P

S

Q

P

A

A

Q

G

P

T

N

Y

F

E

A

-

F

T

D

K

R

D

D

G

G

H

N

Y

P

L

L

-

S

-

F

-

D

-

N

-

I

-

T

-

S

-

V

-

P

-

R

-

G

-

N

-

A

-

G

-
x
G

-

Q

-

P

-

G

-

W

-

M

-

L

-

K

-

C

-

K

-

G

-

W

-

E

-

T

-

D

-

A

-

D

-

S

-

Y

V

V

L

Y

V

F

T

T

S

I

T

A

D

A

S

N

T

M

F

W

N

P

R

Q

L

I

A

C

E

D

A

M

M

I

N

D

R

K

L

P

D

E

L

W

L

K

E

D

N

D

E

P

K

A

F

Y

S

N

V

T

N

F

A

E

A

G

R

R

L

V

K

D

H

K

N

L

D

M

E

D

M

I

D

F

A

S

I

F

V

I

S

E

Q

T

W

K

I

F

A

A

S

D

K

K

T

D

R

K

D

F

D

E

V

V

L

T

D

E

I

W

L

A

D

A

T

Q

H

Y

G

G

V

I

P

P

V

C

S

G

P

P

I

V

L

M

S

S

I

M

R

K

D

E

I

L

F

A

E

H

H

D

P

P

Q

S

F

L

K

Q

A

K

R

V

E

G

N

T

I

V

V

V

E

E

V

V

H

V

H

D

P

E

R

I

L

R

G

G

K

N

V

H

K

L

V

T

P

V

G

G

I

A

I

P

P

F

K

K

F

F

E

S

K

G

T

F

P

Q

G

P

R

E

I

I

R

T

H

R

I

-

A

A

P

P

D

L

L

L

G

G

E

E

H

H

N

T

Y

D

E

E

I

V

L

L

T

-

K

K

M

E

L

L

G

G

F

L

T

D

E

D

E

A

E

K

C

I

K

K

Q

E

L

L

E

H

E

A

K

K

G

Q

V

V

I

V

active site: Q16 (≠ I19), E139 (≠ G141), D168 (= D170), G259 (vs. gap), G260 (vs. gap)
binding coenzyme a: H14 (≠ T17), V15 (≠ M18), Q16 (≠ I19), R37 (≠ I40), M73 (≠ L75), N95 (= N97), F96 (= F98), R103 (≠ K105), M104 (≠ W106), V137 (≠ G139), Y138 (≠ F140), D168 (= D170), M199 (≠ L203)
binding oxalic acid: G259 (vs. gap), G260 (vs. gap)

2gd6A The 1,1-proton transfer reaction mechanism by alpha-methylacyl-coa racemase is catalyzed by an aspartate/histidine pair and involves a smooth, methionine-rich surface for binding the fatty acyl moiety (see paper)
26% identity, 89% coverage: 6:361/400 of query aligns to 3:331/354 of 2gd6A

query
sites
2gd6A

P

P

L

L

E

S

G

G

I

L

K

R

I

V

L

V

D

E

V

L

S

A

T

G

M
x
I

I
x
G

A

P

A

G

P

P

Y

H

G

A

A

A

V

M

L

I

L

L

G

G

D

D

F

L

G

G

A

A

E

D

V

V

I

V

K

R

V

I

E

D

I

-

P

-

G

-

K

-

G

-

D

-

T

-

L

-

R

-

H

-

V

-

G

-

P

-

F

-

A

-

D

-

G

-

E
x
R

P

P

L

I

R

S

W

R

S

D

G

A

L

M

S

L

R

R

N

N

K

R

S

I

L

V

T

T

L
x
A

D
|
D

L
|
L

H
x
K

K

S

E

D

E

Q

A

G

K

L

D

E

I

L

F

A

K

L

K

K

L

L

A

I

A

A

Q

K

V

A

D

D

V

V

I

L

I

I

E

E

N
x
G

F
x
Y

R
|
R

P

P

G

G

T
x
V

L

T

E

E

K
x
R

W

L

D

G

V

L

G

G

Y

P

D

E

V

E

L

C

K

A

E

K

I

V

N

N

P

D

R

R

L

L

I

I

M

Y

I

A

R

R

V

M

S

T

G

G

Y

W

G

G

Q

Q

T

T

G

G

P

P

F

R

K

S

D

Q

K

Q

A

A

G
|
G

F
x
H

G
x
D

T

I

P

N

A
x
Y

T

I

A

S

F

L

S

N

G

G

F

I

T

L

Y

H

I

A

Q

I

G

G

F

R

T

G

D

D

R

E

H

R

P

P

V

V

S

P

P

P

S

L

F

N

S

L

L

V

T

G

D
|
D

Y

F

I

G

A

G

G

G

-

S

I

M

Y

F

V

L

A

L

F

V

A

G

T

I

V

L

T

A

A

A

M

L

Y

W

Y

E

R

R

D

-

T

-

H

Q

S

S

E

S

G

G

T

K

G

G

Q

Q

M

V

V

V

D

D

L

A

A

A

L
x
M

Y

V

E

D

S

G

V

S

F

S

R

V

M

L

L

I

E

Q

F

M

L

M

V

W

A

A

E

M

Y

R

D

A

K

T

L

G

G

M

K

W

V

T

R

D

E

T

R

R

S

G

P

A

G

N

L

M

S

L

G

D

H
x
G

S
x
G

S

A

P

P

-

Y

A

Y

G

D

T

T

Y

Y

E

E

T

C

K

A

D

D

G

G

H

R

Y

Y

L

-

V

V

L

A

V

V

T

G

S

A

T

I

D

E

S

P

T

Q

F

F

N

Y

R

A

L

A

A

M

E

L

A

A

M

G

N

L

R

G

L

L

D

D

L

A

E

E

N

L

E

P

K

P

F

Q

S

N

V

-

N

D

A

R

A

A

R

R

L

W

K

P

H

-

N

-

D

-

E

E

M

L

D

R

A

A

I

L

V

L

S

T

Q

E

W

A

I

F

A

A

S

S

K

H

T

D

R

R

D

D

D

H

V

W

L

G

D

A

I

V

L

F

D

A

T

N

H

S

G

D

V

A

P

C

V

V

S

T

P

P

I

V

L

L

S

A

I

F

R

G

D

E

I

V

F

H

E

N

H

E

P

P

Q

H

F

I

K

I

A

E

R

R

E

N

N

T

I

F

V

Y

E

E

V

A

H

N

H

G

P

-

R

-

L

-

G

G

K

W

V

Q

K

P

V

M

P

P

G

A

I

-

P

P

K

R

F

F

E

S

K

R

T

T

active site: G16 (≠ I19), D121 (≠ G141), D150 (= D170), G213 (≠ H234), G214 (≠ S235)
binding acetyl coenzyme *a: I15 (≠ M18), R37 (≠ E57), A53 (≠ L73), D54 (= D74), L55 (= L75), K56 (≠ H76), G77 (≠ N97), Y78 (≠ F98), R79 (= R99), V82 (≠ T102), R85 (≠ K105), G119 (= G139), H120 (≠ F140), Y124 (≠ A144), D150 (= D170), M182 (≠ L203)

Query Sequence

>WP_035703129.1 NCBI__GCF_000691145.1:WP_035703129.1
MNKNTPLEGIKILDVSTMIAAPYGAVLLGDFGAEVIKVEIPGKGDTLRHVGPFADGEPLR
WSGLSRNKKSLTLDLHKEEAKDIFKKLAAQVDVIIENFRPGTLEKWDVGYDVLKEINPRL
IMIRVSGYGQTGPFKDKAGFGTPATAFSGFTYIQGFTDRHPVSPSFSLTDYIAGIYVAFA
TVTAMYYRDTHSEGTGQMVDLALYESVFRMLEFLVAEYDKLGKVRERSPGLSGHSSPAGT
YETKDGHYLVLVTSTDSTFNRLAEAMNRLDLLENEKFSVNAARLKHNDEMDAIVSQWIAS
KTRDDVLDILDTHGVPVSPILSIRDIFEHPQFKARENIVEVHHPRLGKVKVPGIIPKFEK
TPGRIRHIAPDLGEHNYEILTKMLGFTEEECKQLEEKGVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory