SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_035704030.1 NCBI__GCF_000691145.1:WP_035704030.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
35% identity, 99% coverage: 4:444/445 of query aligns to 2:448/469 of P46349

query
sites
P46349

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G33 (= G35) mutation to D: Lack of activity.
G42 (= G44) mutation to S: Lack of activity.
G301 (= G300) mutation to V: Lack of activity.
G338 (≠ T337) mutation to E: Lack of activity.
F341 (= F340) mutation to S: Lack of activity.
G414 (= G404) mutation to R: Lack of activity.

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
37% identity, 93% coverage: 5:416/445 of query aligns to 2:418/467 of Q88CZ8

query
sites
Q88CZ8

Q

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T27 (= T30) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ M100) mutation to A: Retains 80% of wild-type activity.
K156 (= K158) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F209) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E215) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P24207 Phenylalanine-specific permease; Phenylalanine:H(+) symporter PheP from Escherichia coli (strain K12) (see 3 papers)
38% identity, 89% coverage: 2:398/445 of query aligns to 11:412/458 of P24207

query
sites
P24207

S

T

A

A

N

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N

E

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Q

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Q

R

E

E

P

T

Y

Q

F

F

K

K

V

A

W

L

L

L

F

Y

P

P

Y

F

R26 (= R17) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P45) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (≠ L77) mutation to L: No effect on phenylalanine transport activity.
F90 (≠ L80) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ Q82) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (≠ A84) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L85) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ R88) mutation to L: No effect on phenylalanine transport activity.
F101 (= F91) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W95) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y97) mutation to L: No effect on phenylalanine transport activity.
W108 (≠ C98) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W101) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E108) mutation E->G,L,V,N: Loss of activity.
K168 (= K158) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E215) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R241) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P330) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.

Sites not aligning to the query:

442 P→A: 46% of wild-type phenylalanine transport activity.; P→G: 52% of wild-type phenylalanine transport activity.; P→L: 43% of wild-type phenylalanine transport activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
34% identity, 94% coverage: 1:418/445 of query aligns to 2:424/457 of P15993

query
sites
P15993

M

M

S

E

A

G

N

Q

Q

Q

E

H

Q

G

H

E

E

Q

L

L

K

K

K

R

L

G

L

L

S

K

P

N

R

R

H

H

I

I

R

Q

M

L

I

I

A

A

M

L

G

G

V

A

I

I

G

G

T

T

G

G

I

L

F

F

K

L

G

G

S

S

A

A

D

S

T

V

I

I

G

Q

L

S

A

A

G

G

P

P

G

G

V

I

I

I

F

L

S

G

Y

Y

V

A

F

I

A

A

G

G

L

F

L

I

L

A

L

F

I

L

V

I

M

M

A

R

A

Q

L

L

A

G

E

E

M

M

A

V

I

V

V

E

Y

E

P

P

-

V

G

A

N

G

N

S

L

F

R

S

D

H

L

F

I

A

Q

Y

I

K

A

Y

L

W

G

G

Q

S

R

F

F

A

S

G

F

F

V

A

V

S

G

G

W

W

V

N

Y

Y

C

W

F

V

M

L

W
x
Y

L

V

T

L

V

V

C

A

V

M

I

A

E

E

I

L

I

T

A

A

A

V

G

G

S

K

L

Y

L

I

Q

Q

Y

F

W

W

M

Y

P

P

N

E

V

I

P

P

L

T

W

W

S

V

L

S

C

A

L

A

L

V

C

F

S

F

L

V

V

I

I

N

G

A

I

I

N

N

L

L

N

T

N

N

V

V

K

K

Y

V

F

F

G

G

E

E

I

M

E

E

F

F

W

W

F

F

A

A

G

I

M

I

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K

I

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F

I

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A

I

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V

A

F

M

I

I

L

F

G

G

A

G

A

W

L

L

F

F

G

S

I

G

I

N

P

G

S

G

E

P

Q

Q

S

A

T

T

-

V

P

S

V

N

L

L

T

W

N

D

Y

Q

Q

G

N

G

F

F

V

L

P

P

N

H

G

G

W

F

G

T

A

G

V

L

F

V

A

M

S

M

L

M

L

A

V

I

I

M

F

F

S

S

Y

F

G

G

S

L

E

E

L

L

I

V

G

G

L

I

T

T

I

A

T

A

E

E

T

A

K

D

D

N

A

P

E

E

K

Q

V

S

L

I

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P

G

K

V

A

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T

K

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Q

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V

M

I

W

Y

R

R

I

I

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L

I

F

F

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Y

T

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P

S

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L

A

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I

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C

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S

L

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M

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P

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W

N

T

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R

I

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A

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S

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P

F

F

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Q

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V

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F

S

H

A

E

A

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G

M

D

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G

F

A

V

S

A

H

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F

A

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N

F

I

V

V

L

V

I

L

T

T

A

A

V

A

L

L

S

S

A

V

A

Y

N

N

S

S

G

C

I

V

Y

Y

G

C

T

N

T

S

R

R

M

M

I

L

H

F

S

G

L

L

S

A

K

Q

S

Q

D

G

G

N

G

A

P

P

K

K

K

A

L

L

A

A

Q

S

V

V

N

D

K

K

R

R

G

G

V

V

P

P

I

V

L

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S

T

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I

W

L

V

V

T

S

V

A

L

L

F

V

L

T

L

A

I

L

G

C

T

V

F

L

F

I

A

N

Y

Y

L

L

Y

A

P

P

D

E

Q

S

I

A

F

F

S

G

Y

L

M

L

L

M

A

A

I

L

P

V

G

V

F

S

A

A

V

L

S

V

L

I

I

N

W

W

I

A

S

M

I

I

C

S

L

L

A

A

H

H

L

M

K

K

L

F

R

R

P

-

R

R

Y

A

P

K

Q

Q

E

E

-

Q

-

G

-

V

P

T

Y

R

F

F

K

P

V

A

W

L

L

L

F

Y

P

P

Y

L

F

G

T

N

L

W

F

I

A

C

G

L

L

L

V

F

L

M

S

A

I

A

S

V

F

L

V

V

L

I

F

M

L

L

F

M

R

T

P

P

Y103 (≠ W101) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
33% identity, 86% coverage: 2:385/445 of query aligns to 5:400/489 of P25737

query
sites
P25737

S

T

A

K

N

T

Q

T

E

E

Q

A

H

P

E

G

L

L

K

R

L

E

L

L

S

K

P

A

R

R

H

H

I

L

R

T

M

M

I

I

A

A

M

I

G

G

V

S

I

I

G

G

T

T

G

G

I

L

F

F

K

V

G

A

S

S

A

G

D

A

T

T

I

I

G

S

L

Q

A

A

G

G

P

P

G

G

-

G

V

A

I

L

F

L

S

S

Y

Y

V

M

F

L

A

I

G

G

L

L

L

M

L

V

L

Y

I

F

V

L

M

M

A

T

A

S

L

L

A

G

E

E

M

L

A

A

I

A

V

Y

Y

M

P

P

-

V

G

S

N

G

N

S

L

F

R

A

D

T

L

Y

I

G

Q

Q

I

N

A

Y

L

V

G

E

Q

E

R

G

F

F

S

G

F

F

V

A

V

L

G

G

W

W

V

N

Y
|
Y

C

W

F

Y

M

N

W
|
W

L

A

T

V

V

T

C

I

V

A

I

V

E

D

I

L

I

V

A

A

G

Q

S

L

L

V

L

M

Q

S

Y

W

W

W

M

F

P

P

N

D

V

T

P

P

L

G

W

W

S

I

L

W

C

S

L

A

L

L

C

F

S

L

L

G

L

V

V

I

I

F

G

L

I

L

N

N

L

Y

N

I

N

S

V

V

K

R

Y

G

F

F

G

G

E

E

I

A

E

E

F

Y

W

W

F

F

A

S

G

L

M

I

K
|
K

I

V

F

T

V

T

I

V

I

I

V

V

F

F

I

I

L

V

G

G

A

V

A

L

L

M

L

I

F

I

G

G

I

I

I

F

P

K

S

G

E

A

Q

Q

S

P

T

-

P

-

V

-

L

-

T

A

N

G

Y

W

Q

S

N

N

F

W

V

T

-

I

-

G

-

E

-

A

-

P

-

F

P

A

N

G

G

G

W

F

G

A

A

A

V

M

F

I

A

G

S

V

L

A

L

M

V

I

V

V

I

G

F
|
F

S

S

Y

F

G

Q

G

G

S

T

E
|
E

L

L

I

I

G

G

L

I

T

A

I

A

T

G

E
|
E

T

S

K

E

D

D

A

P

E

A

K

K

V

N

L

I

P

P

G

R

V

A

V

V

K

R

S

Q

M

V

M

F

W

W

R

R

I

I

I

L

L

L

F

F

Y

Y

T

V

L

F

P

A

I

I

L

L

I

I

C

S

G

L

L

I

I

I

P

P

W

Y

N

T

Q

D

-

P

-

S

-

L

-

R

-

N

-
x
D

-

V

I

K

D
|
D

P

I

N

S

N

V

S

S

P

P

F

F

V

T

Q

L

V

V

F

F

S

Q

A

H

A

A

G

G

M

L

Q

L

G

S

A

A

S

A

H

A

F

V

I

M

N

N

F

A

V

V

L

I

I

L

T

T

A

A

V

V

L

L

S

S

A

A

A

G

N

N

S

S

G

G

I

M

Y

Y

G

A

T

S

T

T

R

R

M

M

I

L

H

Y

S

T

L

L

S

A

K

C

S

D

D

G

G

K

G

A

P

P

K

R

K

I

L

F

A

A

Q

K

V

L

N

S

K

R

R

G

G

G

V

V

P

P

I

R

L

N

S

A

L

L

W

Y

V

A

T

T

V

T

L

V

F

I

L

A

L

G

I

L

G

-

T

C

F

F

F

L

A

T

Y

S

L

M

Y

F

P

G

D

N

Q

Q

-

T

I

V

F

Y

S

L

Y

W

M

L

L

L

A

N

I

T

P

S

G

G

F

M

A

T

V

G

S

F

L

I

I

A

W

W

I

L

S

G

I

I

C

A

L

I

A

S

H

H

L

Y

K

R

L

F

R

R

P

R

R

G

Y

Y

Y102 (= Y97) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W101) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K158) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F209) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E215) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E223) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (vs. gap) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (= D263) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
438 E→A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
443 D→A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
446 D→A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 91% coverage: 6:409/445 of query aligns to 80:508/590 of P04817

query
sites
P04817

E

Q

Q

N

H

A

E

E

L

V

K

K

K

R

L

E

L

L

S

K

P

Q

R

R

H

H

I

I

R

G

M

M

I

I

A

A

M

L

G

G

V

T

I

I

G

G

T

T

G

G

I

L

F

F

K

I

G

G

S

L

A

S

D

T

T
x
P

I

L

G

T

L

N

A

A

G

G

P

P

-

V

G

G

V

A

I

L

F

I

S

S

Y

Y

V

L

F

F

A

M

G

G

L

S

L

L

L

A

L

Y

I

S

V

V

M

T

A

Q

A

S

L

L

A

G

E

E

M

M

A

A

I

T

V

F

Y

I

P
|
P

-
x
V

G

T

N

S

N
x
S

L

F

R

T

D

V

L

F

I

S

Q

Q

I

R

A

F

L

L

G

S

Q

P

R

A

F

F

S

G

F

A

V

A

V

N

G

G

W

Y

V

M

Y
|
Y

C

W

F

F

M

S

W

W

L

A

T

I

V

T

C

F

V

A

I

L

E

E

I

L

I

S

A

V

G

G

S

Q

L

V

L

I

Q

Q

Y

F

W

W

M

T

P

Y

N

K

V

V

P

P

L

L

W

A

S

A

L

W

C

I

L

S

L

I

C

F

S

W

L

V

L

I

V

I

I

T

G

I

I

M

N

N

L

L

N

F

N

P

V

V

K

K

Y

Y

F

Y

G

G

E

E

I

F

E

E

F

F

W

W

F

V

A

A

G

S

M

I

K

K

I

V

F

L

V

A

I

I

V

G

F

F

I

L

I

I

L

Y

G

C

A

F

A

C

L

M

L

V

F

C

G

G

I

-

P

-

S

A

E

G

Q

V

S

T

T

G

P

P

V

V

L

G

T

F

N

R

Y

Y

Q

W

N

R

F

-

V

N

P

P

N

G

G

A

W

W

G

G

-

P

-

G

-

I

-

S

-

K

-

D

-

K

-

N

-

E

-

G

-

R

-

F

-

L

A

G

V

W

F

V

A

S

S

S

L

L

L

I

V

N

V

A

I

A

F

F

S

T

Y

F

G

Q

G

G

S

T

E

E

L

L

I

V

G

G

L

I

T

T

I

A

T
x
G

E

E

T

A

K

A

D

N

A
x
P

E

R

K

K

V

S

L

V

P

P

G

R

V

A

V

I

K

K

S

K

M

V

W

F

R

R

I

I

I

L

L

T

F

F

Y

Y

T

I

L

G

P

S

I

L

L

L

I

F

I

I

C

G

G

L

L

L

I

V

P

P

W

Y

N

N

Q

-

I

-

D

D

P

P

-

K

-

L

-

T

-

Q

-

S

-
x
T

-
x
S

-
x
Y

-

V

N

S

N

T

S

S

P

P

F

F

V

I

Q

I

V

A

F

I

S

E

A

N

A

S

G

G

M

T

Q

K

G

V

A

L

S

P

H

H

F

I

I

F

N

N

F

A

V

V

L

I

I

L

T

T

A

T

V

I

L

I

S

S

A

A

N

N

S

S

G

N

I

I

Y

Y

G

V

T

G

T

S

R

R

M

I

I

L

H

F

S

G

L

L

S

S

K

K

S

N

D

K

G

L

G

A

P

P

K

K

K

F

L

L

A

S

Q

R

V

T

N

T

K

K

R

G

G

G

V

V

P

P

I

Y

L

I

S

A

L

V

W

F

V

V

T

T

V

A

L

A

F

F

L

G

L

A

I

L

G

A

T

Y

F

M

F

E

A

T

Y

S

L

T

Y

G

P

G

D

D

Q

K

I

V

F

F

S

E

Y
x
W

M

L

L

L

A

N

I

I

P

T

G

G

F

V

A

A

V

G

S
x
F

L

F

I

A

W

W

I

L

S

F

I

I

C

S

L

I

A

S

H

H

-

I

-

R

-

F

-

M

-

Q

-

A

L

L

K

K

L

Y

R

R

P

G

R

I

Y

S

P

R

Q

D

E

E

P

L

Y

P

F

F

K

K

V

A

W

K

L

L

F

M

P

P

Y

G

F

L

T

A

L

Y

F

Y

A

A

G

A

L

T

V

F

L

M

S

T

I

I

P113 (≠ T39) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (= P73) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (vs. gap) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (≠ N76) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y97) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ T222) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (≠ A227) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ Y358) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ S368) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 86% coverage: 1:382/445 of query aligns to 136:534/663 of P48813

query
sites
P48813

M

Q

S

E

A

Q

N

E

Q

Q

E

K

Q

Q

H

E

E

N

L

L

K

K

L

S

L

I

S

K

P

P

R

R

H

H

I

T

R

V

M

M

I

M

A

S

M

L

G

G

G

T

V

G

I

I

G

G

T

T

G

G

I

L

F

L

K

V

G

G

S

N

A

S

D

K

T

V

I

L

G

N

L

N

A

A

G

G

P

P

G

G

-

G

V

L

I

I

F

I

S

G

Y

Y

V

A

F

I

A

M

G

G

L

S

L

C

L

V

L

Y

I

C

V

I

M

I

A

Q

A

A

L

C

A

G

E

E

M

L

A

A

I

V

V

I

Y

Y

P

S

-

D

-

L

-

I

-

G

G

G

N

F

N

N

L

T

R

Y

D

P

L

L

-

F

-

L

I

V

Q

D

I

P

A

A

L

L

G

G

Q

-

R

-

F

-

S

-

F

F

V

S

V

V

G

A

W

W

V

L

Y

F

C

C

F

L

M

Q

W

W

L

L

T

C

V

V

C

C

V

P

I

L

E

E

I

L

I

V

A

T

A

A

G

S

S

M

L

T

L

I

Q

K

Y

Y

W

W

M

T

P

T

N

S

V

V

P

N

L

P

W

D

S

V

L

F

C

V

L

V

L

I

C

F

S

Y

L

V

L

L

V

I

I

V

G

V

I

I

N

N

L

V

N

F

N

G

V

A

K

K

Y

G

F

Y

G

A

E

E

I

A

E

D

F

F

W

F

F

F

A

N

G

C

M

C

K

K

I

I

F

L

V

M

I

I

I

V

V

G

F

F

I

F

I

I

L

L

-

A

-

I

-

D

-

C

G

G

A

G

A

A

L

G

L

T

F

D

G

G

I

Y

I

I

P

G

S

S

E

K

-

Y

-

W

-

R

-

D

-

P

-

G

-

A

-

F

-

R

Q

G

S

D

T

T

P

P

V

I

L

-

T

-

N

-

Y

-

Q

Q

N

R

F

F

V

-

P

-

N

-

G

-

W

-

G

K

A

G

V

V

F

V

A

A

S

T

L

F

L

V

V

T

V

A

I

A

F

F

S

A

Y

F

G

G

G

M

S

S

E

E

L

Q

I

L

G

A

L

M

T

T

I

A

T

S

E

E

T

Q

K

S

D

N

A

P

E

R

K

K

V

A

L

I

P

P

G

S

V

A

K

K

S

K

M

M

M

I

W

Y

R

R

I

I

I

L

L

F

F

V

Y

F

T

L

L

A

P

S

I

L

L

T

I

L

I

V

C

G

G

F

L

L

I

V

P

P

W

Y

N

T

Q

S

I

D

D

Q

-

L

-

G

-

A

-

G

-

S

-

A

P

T

N

K

N

A

S

S

P

P

F

Y

V

V

Q

I

V

A

F

V

S

S

A

S

A

H

G

G

M

V

Q

R

G

V

A

V

S

P

H

H

F

F

I

I

N

N

F

A

V

V

L

I

I

L

T

L

A

S

V

V

L

L

S

S

A

V

A

A

N

N

S

G

G

A

I

F

Y

Y

G

T

T

S

R

R

M

I

I

L

H

M

S

S

L

L

S

A

K

K

S

Q

D

G

G

N

G

A

P

P

K

K

K

C

L

F

A

D

Q

Y

V

I

N

D

K

R

R

E

G

G

V

R

P

P

I

A

L

A

S

A

L

M

W

L

V

V

T

S

V

A

L

L

F

F

L

G

L

V

I

I

G

A

T

F

F

C

F

A

A

S

Y

S

L

K

Y

K

P

E

D

E

Q

D

I

V

F

F

S

T

Y

W

M

L

L

L

A

A

I

I

P

S

G

G

F

L

A

S

V

Q

S

L

L

F

I

T

W

W

I

I

S

T

I

I

C

C

L

L

A

S

H

H

L

I

K

R

L

F

R

R

Sites not aligning to the query:

132 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 87% coverage: 2:390/445 of query aligns to 72:474/587 of Q9URZ4

query
sites
Q9URZ4

S

A

A

T

N

G

Q

E

E

D

Q

G

H

T

E

A

L

L

K

K

K

R

L

S

L

L

S

K

P

S

R

R

H

H

I

M

R

Q

M

M

I

I

A

S

M

I

G

G

V

A

I

I

G

G

T

T

G

G

I

L

F

Y

K

V

G

G

S

S

A

G

D

S

T

S

I

L

G

A

L

D

A

G

G

G

P

P

G

A

-

S

V

V

I

I

F

I

S

N

Y

Y

V

S

F

L

A

I

G

G

L

I

L

M

L

F

I

F

V

I

M

V

A

Y

A

A

L

L

A

G

E

E

M

M

A

A

I

V

V

A

Y

Y

P

P

-

V

G

A

N

G

L

F

R

N

D

T

L

Y

I

A

Q

T

I

R

A

F

L

I

G

D

Q

P

R

A

F

W

S

G

F

F

V

A

V

V

G

S

W

W

V

N

Y

Y

C

F

F

I

M

N

W

Y

L

F

T

V

V

T

C

F

V

P

I

L

E

E

I

L

I

T

A

T

A

C

G

A

S

I

L

T

L

F

Q

R

Y

Y

W

W

M

T

P

D

-

I

N

N

V

S

P

A

L

A

W

W

S

I

L

S

C

I

L

F

L

L

C

-

S

-

L

V

V

I

I

I

G

I

I

V

N

N

L

L

N

F

N

G

V

V

K

R

Y

A

F

Y

G

G

E

E

I

V

E

E

F

F

W

I

F

L

A

S

G

T

M

V

K

K

I

V

F

V

V

A

I

T

I

F

V

G

F

F

I

I

L

L

G

A

A

I

L

I

L

N

F

C

G

G

I

G

I

V

P

P

S

T

E

D

Q

H

S

R

T

G

P

Y

V

I

L

G

T

G

N

S

Y

I

Q

I

N

K

F

H

V

K

P

P

-

F

-

R

N

H

G

G

W

F

G

K

A

G

V

F

F

C

A

S

S

V

L

F

L

T

V

T

V

A

I

A

F

F

S

S

Y

F

G

S

G

G

S

T

E

E

L

V

I

I

G

G

L

L

T

A

I

A

T

A

E

E

T

V

K

D

D

N

A

P

E

Q

K

K

V

A

L

L

P

P

G

H

V

A

V

V

K

K

S

Q

M

V

M

F

W

W

R

R

I

I

I

A

L

I

F

F

Y

Y

T

V

L

V

P

S

I

L

L

I

I

L

I

I

C

G

G

L

L

L

I

I

P

S

W

P

N

D

Q

-

I

-

D

D

P

P

N

N

-

L

-

M

-

G

-

N

-

G

-

S

-

T

-

S

N

V

S

S

P

P

F

F

V

V

Q

L

V

A

F

I

S

K

A

E

A

A

G

N

M

I

Q

K

G

G

A

L

S

P

H

S

F

V

I

F

N

N

F

A

V

V

L

I

I

I

T

I

A

S

V

V

L

V

S

S

A

V

A

T

N

N

S

S

G

S

I

T

Y

Y

G

T

T

A

T

G

R

R

M

T

I

L

H

H

S

G

L

M

S

A

K

N

S

L

D

K

G

Q

G

A

P

P

K

S

K

F

L

F

A

K

Q

Y

V

T

N

D

K

R

R

L

G

G

V

R

P

P

I

L

L

L

S

A

L

M

W

I

V

V

T

V

V

L

L

L

F

F

L

-

I

-

G

-

T

G

F

F

A

A

Y

Y

L

I

Y

N

P

E

-

A

-

D

-

K

-

N

-

G

-

N

-

D

-

V

-

S

D

D

Q

T

I

V

F

F

S

N

Y

W

M

L

L

L

A

A

I

L

P

S

G

G

F

L

A

S

V

N

S

F

L

F

I

T

W

W

I

G

S

S

I

I

C

C

L

L

A

C

H

H

L

I

K

I

L

F

R

R

P

L

R

A

Y

F

P

K

Q

K

E

Q

P

G

Y

H

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
30% identity, 84% coverage: 7:382/445 of query aligns to 83:475/602 of P19145

query
sites
P19145

Q

Q

H

T

E

P

L

L

K

K

K

H

L

H

L

L

S

K

P

N

R

R

H

H

I

L

R

Q

M

M

I

I

A

A

M

I

G

G

V

A

I

I

G

G

T

T

G

G

I

L

F

L

K

V

G

G

S

S

A

G

D

T

T

A

I

L

G

R

L

T

A

G

G

G

P

P

G

A

-

S

V

L

I

L

F

I

S

G

Y

W

V

G

F

S

A

T

G

G

L

T

L

M

L

I

L

Y

I

A

V

M

M

V

A

M

A

A

L

L

A

G

E

E

M

L

A

A

I

V

V

I

Y

F

P

P

-

I

G

S

N

G

L

F

R

T

D

T

L

Y

I

A

Q

T

I

R

A

F

L

I

G

D

Q

E

R

S

F

F

S

G

F

Y

V

A

V

N

G

N

W

F

V

N

Y

Y

C

M

F

L

M

Q

W

W

L

L

T

V

V

V

C

L

V

P

I

L

E

E

I

I

I

V

A

S

A

A

G

S

S

I

L

T

L

V

Q

N

Y

F

W

W

M

G

P

T

N

D

V

-

P

P

L

K

W

Y

S

R

-

D

-

G

L

F

C

V

L

A

L

L

C

F

S

W

L

L

L

A

V

I

I

V

G

I

I

I

N

N

L

M

N

F

N

G

V

V

K

K

Y

G

F

Y

G

G

E

E

I

A

E

E

F

F

W

V

F

F

A

S

G

F

M

I

K

K

I

V

F

I

V

T

I

V

V

G

F

F

I

I

L

L

G

G

A

-

L

I

F

L

G

N

I

C

I

G

P

G

S

G

E

P

Q

T

S

G

T

G

P

Y

V

I

L

G

T

G

N

K

Y

Y

Q

W

N

H

-

D

-

P

-

G

-

A

F

F

V

A

P

G

N

D

G

T

W

P

G

G

A

A

V

K

F

F

A

K

S

G

L

V

L

C

V

S

V

V

I

F

-

V

-

T

-

A

F

F

S

S

Y

F

G
x
A

G

G

S

S

E

E

L

L

I

V

G

G

L

L

T

A

I

A

T

S

E

E

T

S

K

V

D

E

A

P

E

R

K

K

V

S

L

V

P

P

G

K

V

A

K

K

S

Q

M

V

M

F

W

W

R

R

I

I

I

T

L

L

F

F

Y

Y

T

I

L

L

P

S

I

L

L

L

I

M

I

I

C

G

G

L

L

L

I

V

P

P

W

Y

N

N

Q

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

I

V

D

D

P

A

N

A

S

S

P

P

F

F

V

V

Q

I

V

A

F

I

S

K

A

T

A

H

G

G

M

I

Q

K

G

G

A

L

S

P

H

S

F

V

I

V

N

N

F

V

V

V

L

I

I

L

T

I

A

A

V

V

L

L

S

S

A

V

A

G

N

N

S

S

G

A

I

I

Y

Y

G

A

T

C

T

S

R

R

M

T

I

M

H

V

S

A

L

L

S

A

K

E

S

Q

D

R

G

F

G

L

P

P

K

E

K

I

L

F

A

S

Q

Y

V

V

N

D

K

R

R

K

G

G

V

R

P

P

I

L

L

V

S

G

L

I

W

A

V

V

T

T

V

S

L

A

F

F

L

G

L

L

I

I

G

A

T

F

F

V

F

A

A

A

Y

S

L

K

Y

K

P

E

D

G

Q

E

I

V

F

F

S

N

Y

W

M

L

L

L

A

A

I

L

P

S

G

G

F

L

A

S

V

S

S

L

L

F

I

T

W

W

I

G

S

G

I

I

C

C

L

I

A

C

H

H

L

I

K

R

L

F

R

R

A297 (≠ G212) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
23% identity, 94% coverage: 2:419/445 of query aligns to 269:774/852 of Q03770

query
sites
Q03770

S

S

A

R

N

A

Q

T

E

R

Q

K

H

Y

E

H

L

I

K

Q

K

R

L

K

L

L

S

K

P

V

R

R

H

H

I

I

R

Q

M

M

I

L

A

S

M

I

G

G

G

A

V

C

I

F

G

S

T

V

G

G

I

L

F

F

K

L

G

T

S

S

A

G

D

K

T

A

I

F

G

S

L

I

A

A

G

G

P

P

-

F

G

G

V

T

I

L

F

L

S

G

Y

F

V

G

F

L

A

T

G

G

L

S

L

I

L

L

I

A

V

T

M

M

A

L

A

S

L

F

A

T

E

E

M

L

A

S

I

T

V

L

Y

I

P

P

-

V

G

S

N

S

N

G

L

F

R

S

D

G

L

L

I

A

Q

S

I

R

A

F

L

V

G

E

Q

D

R

A

F

F

S

G

F

F

V

A

V

L

G

G

W

W

V

T

Y

Y

C

-

F

-

M

-

W

W

L

I

T

S

V

-

C

C

V

M

I

L

E

A

I

L

I

P

A

A

A

Q

G

V

S

S

L

S

Q
x
T

Y

F

W

Y

M

L

P

S

-

Y

-

N

N

N

V

V

P

N

L

I

W

S

S

K

-

G

-

V

-

T

-

A

-

G

L

F

C

I

L

T

L

L

C

F

S

S

L

A

L

F

V

S

I

I

G

V

I

V

N

N

L

L

N

L

N

D

V

V

K

S

Y

I

F

M

G

G

E

E

I

I

E

-

F

V

W

Y

F

V

A

A

G

G

M

I

K

S

-

K

-

V

-

I

-

A

I

I

F

L

V

M

I

V

I

F

V

T

F

M

I

I

L

L

G

N

A

A

-

G

-

H

-

G

-

N

-

D

-

I

-

H

-

E

A

G

L

V

L

G

F

F

G

R

I

Y

I

W

P

D

S

S

E

S

Q

K

S

S

T

V

P

R

V

N

L

L

T

T

N

-

Y

Y

Q

G

N

L

F

Y

V

R

P

P

N

T

-

F

-

D

-

L

-

A

-

D

-

A

G

G

W

E

G

G

-

S

-

K

-

G

-

I

-

S

-

G

-

P

-

K

-

G

-

R

-

F

-

L

A

A

V

T

F

A

A

S

S

V

L

M

L

L

V

I

V

S

I

T

F

F

S

A

Y

F

G

S

G

G

S

V

E

E

L

M

I

T

G

F

L

L

T

A

I

S

T

G

E

E

T

A

K

I

D

N

A

P

E

R

K

K

V

T

L

I

P

P

G

S

V

A

V

T

K

K

S

R

M

T

M

F

W

S

R

I

I

V

I

L

L

I

F

S

Y

Y

T

V

L

F

P

L

I

I

L

F

I

S

I

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

-

A

-

I

-

K

-

G

-

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

-

T

-

N

C

C

-

R

G

G

L

G

I

I

P

D

W

Y

N

R

Q

Q

I

I

D

S

P

V

N

G

-

T

-

G

-

Y

N

S

S

S

P

P

F

W

V

V

Q

V

V

A

F

L

S

Q

A

N

A

F

G

G

M

L

Q

C

G

T

A

F

S

A

H

S

F

A

I

F

N

N

F

A

V

I

L

L

I

I

T

F

A

F

V

T

L

A

S

T

A

A

A

G

N

I

S

S

G

S

I

L

Y

F

G

S

T

C

T

S

R

R

M

T

I

L

H

Y

S

A

L

M

S

S

K

V

S

Q

D

R

G

K

G

A

P

P

K

P

K

V

L

F

A

E

Q

I

V

C

N

S

K

K

R

R

G

G

V

V

P

P

I

Y

L

V

S

S

L

V

W

I

V

F

T

S

V

S

L

L

F

F

L

S

L

V

I

I

G

-

T

-

F

-

A

A

Y

Y

L

I

Y

A

P

V

D

D

Q

Q

I

T

-

A

-

I

-

E

-

N

F

F

S

D

Y

V

M

L

L

A

A

N

I

V

P

S

G

S

F

A

A

S

V

T

S

S

L

I

I

I

W

W

I

M

S

G

I

L

C

N

L

L

A

S

H

F

L

L

K

R

-

F

-

Y

-

A

L

L

R

K

P

Q

R

R

-

K

-

D

-

I

-

S

-

R

-

N

-

D

-

S

Y

Y

P

P

Q

Y

E

K

P

S

Y

P

F

F

K

Q

V

-

W

-

L

-

F

-

P

P

Y

Y

F

L

T

A

L

I

F

Y

A

-

G

G

L

L

V

V

L

G

S

C

I

S

S

L

F

F

V

V

L

I

F

F

L

M

F

G

R

Y

P

P

E

N

T382 (≠ Q117) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

9h76A Bacterial lat transporter basc in complex with l-ala and nb53 (see paper)
23% identity, 90% coverage: 22:420/445 of query aligns to 10:407/430 of 9h76A

query
sites
9h76A

I

V

A

V

M

V

G

G

G

T

V

V

I

I

G
|
G

T
x
A

G
|
G

I

I

F

F

K

F

G

K

S

P

A

T

D

A

T

V

I

Y

G

G

L

A

A

A

G

G

-

A

P

P

G

G

V

L

-

G

I

L

F

L

S

A

Y

W

V

F

F

V

A

A

G

G

L

I

L

T

L

I

I

A

V

G

M

G

A

L

A

T

L

V

A

A

E

E

M

I

A

G

I

T

V

I

Y

Y

P

P

G

Q

N

T

N

G

-

G

L

M

R

M

D

I

L

Y

I

L

Q

E

I

K

A

V

L

Y

G

G

Q

R

R

W

F

L

S

G

F

F

V

L

V

V

G

G

W

W

V

A

Y

Q

C

M

F

V

M

I

W

Y

L

Y

-

P

-

A

T

N

V

I

C

A

V

A

I

L

E

A

I

I

A

F

A

A

G

T

S

Q

L

F

L

V

Q

N

-

L

Y

F

W

A

M

L

P

S

N

D

V

S

P

T

L

I

W

V

S

P

L

T

C

A

L

I

L

L

C

T

S

S

L

I

L

F

V

L

I

M

G

G

I

V

N

N

L

F

N

L

N

G

V

T

K

K

Y

Y

F

S

G

G

E

W

I

I

E

Q

F

T

W

L

F

A

A

T

G

I

M

L

K

K

I

L

F

I

V

P

I

L

I

V

V

V

F

I

I

I

I

V

L

-

G

-

A

A

A

G

L

L

F

Y

-

P

-

G

G

G

I

V

I

I

P

R

-

L

-

V

-

P

-

F

S

S

E

V

Q

E

S

T

T

H

P

P

V

V

L

L

T

T

N

S

Y

F

Q

G

N

S

F

-

V

-

P

-

N

-

G

-

W

-

G

-

A

-

V

-

F

-

A

-

S

A

L

L

L

I

V

A

V

T

I

L

F

F

S

A

Y

Y

G
x
D

G

G

S

W

E

I

L

N

I

V

G

G

L

T

T

L

I

A

T

G

E

E

T

M

K

K

D

N

A

P

E

G

K

K

V

M

L

L

P

P

G

K

V

V

V

I

K

I

S

G

M

G

M

L

W

S

R

I

I

V

I

M

L

A

F

V

Y

Y

T

L

L

L

P

T

I

N

L

I

I

A

I

Y

C

L

G

F

L

V

I

L

P

D

W

S

N

S

Q

Q

I

L

D

A

P

G

N

T

N

D

S

T

P

P

F

A

V

A

Q

L

V

V

F

A

S

S

A

H

A

L

G

F

M

E

Q

G

G

I

A

G

S

S

H

K

F

L

I

V

N

T

F

I

V

G

L

I

I

L

T

I

A

S

V

V

L

F

S

G

A

G

A

I

N

N

S

G

G

Y

I

I

Y

I

G

S

T

G

T

L

R

R

M

V

I

P

H

Y

S

A

L

L

S

A

K

T

S

Q

D

K

G

M

G

L

P

P

K

F

K

S

-

D

-

W

L

F

A

A

Q

R

V

I

N

N

-

P

K

K

R

T

G

N

V

L

P

P

I

I

-

N

-

G

-

L

L

V

S

M

L

L

W

G

V

I

T

A

V

I

L

V

F

M

L

I

L

L

I

T

G

G

T

Q

F

F

F

-

A

-

Y

-

L

-

Y

-

P

-

D

N

Q

Q

I

L

F

T

S

D

Y

L

M

I

L

V

A

F

I

V

P

I

G

W

F

F

A

F

V

I

S

T

L

L

I

T

W

F

I

I

S

A

I

V

C

I

L

I

A

L

H

R

L

-

K

K

L

T

R

Q

P

P

R

D

Y

I

P

-

Q

E

E

R

P

P

Y

Y

F

-

K

R

V

V

W

P

L

F

F

Y

P

P

Y

V

F

I

T

P

L

L

F

I

A

A

G

I

L

I

-

G

V

G

L

L

S

Y

I

I

S

I

F

F

V

N

L

T

F

L

L

I

F

V

R

Q

P

P

E

K

N

N

binding alanine: G17 (= G29), A18 (≠ T30), G19 (= G31), D200 (≠ G212)

6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
23% identity, 90% coverage: 22:420/445 of query aligns to 12:409/433 of 6f2wA

query
sites
6f2wA

I

V

A

V

M

V

G

G

G

T

V
|
V

I

I

G
|
G

T
x
A

G
|
G

I

I

F

F

K

F

G

K

S

P

A

T

D

A

T

V

I

Y

G

G

L

A

A

A

G

G

-

A

P

P

G

G

V

L

-

G

I

L

F

L

S

A

Y

W

V

F

F

V

A

A

G

G

L

I

L

T

L

I

I

A

V

G

M

G

A

L

A

T

L

V

A

A

E

E

M

I

A

G

I

T

V

I

Y

Y

P

P

G

Q

N

T

N

G

-

G

L

M

R

M

D

I

L

Y

I

L

Q

E

I

K

A

V

L

Y

G

G

Q

R

R

W

F

L

S

G

F

F

V

L

V

V

G

G

W

W

V

A

Y

Q

C

M

F

V

M

I

W

Y

L

Y

-

P

-

A

T

N

V

I

C

A

V

A

I

L

E

A

I

I

A

F

A

A

G

T

S

Q

L

F

L

V

Q

N

-

L

Y

F

W

A

M

L

P

S

N

D

V

S

P

T

L

I

W

V

S

P

L

T

C

A

L

I

L

L

C

T

S

S

L

I

L

F

V

L

I

M

G

G

I

V

N

N

L

F

N

L

N

G

V

T

K

K

Y

Y

F

S

G

G

E

W

I

I

E

Q

F

T

W

L

F

A

A

T

G

I

M

L

K

K

I

L

F

I

V

P

I

L

I

V

V

V

F

I

I

I

I

V

L

-

G

-

A

A

A

G

L

L

F

Y

-

P

-

G

G

G

I

V

I

I

P

R

-

L

-

V

-

P

-

F

S

S

E

V

Q

E

S

T

T

H

P

P

V

V

L

L

T

T

N

S

Y

F

Q

G

N

S

F

-

V

-

P

-

N

-

G

-

W

-

G

-

A

-

V

-

F

-

A

-

S

A

L

L

L

I

V

A

V

T

I

L

F
|
F

S
x
A

Y

Y

G
x
D

G

G

S

W

E

I

L

N

I

V

G

G

L

T

T

L

I

A

T

G

E

E

T

M

K

K

D

N

A

P

E

G

K

K

V

M

L

L

P

P

G

K

V

V

V

I

K

I

S

G

M

G

M

L

W

S

R

I

I

V

I

M

L

A

F

V

Y

Y

T

L

L

L

P

T

I

N

L

I

I

A

I

Y

C

L

G

F

L

V

I

L

P

D

W

S

N

S

Q

Q

I

L

D

A

P

G

N

T

N

D

S

T

P

P

F

A

V

A

Q

L

V

V

F

A

S

S

A

H

A

L

G

F

M

E

Q

G

G

I

A

G

S

S

H

K

F

L

I

V

N

T

F

I

V

G

L

I

I

L

T

I

A

S

V

V

L

F

S

G

A

G

A

I

N

N

S

G

G

Y

I

I

Y

I

G

S

T

G

T

L

R

R

M

V

I

P

H

Y

S

A

L

L

S

A

K

T

S

Q

D

K

G

M

G

L

P

P

K

F

K

S

-

D

-

W

L

F

A

A

Q

R

V

I

N

N

-

P

K

K

R

T

G

N

V

L

P

P

I

I

-

N

-

G

-

L

L

V

S

M

L

L

W

G

V

I

T

A

V

I

L

V

F

M

L

I

L

L

I

T

G

G

T

Q

F

F

F

-

A

-

Y

-

L

-

Y

-

P

-

D

N

Q

Q

I

L

F

T

S

D

Y

L

M

I

L

V

A

F

I

V

P

I

G

W

F

F

A

F

V

I

S

T

L

L

I

T

W

F

I

I

S

A

I

V

C

I

L

I

A

L

H

R

L

-

K

K

L

T

R

Q

P

P

R

D

Y

I

P

-

Q

E

E

R

P

P

Y

Y

F

-

K

R

V

V

W

P

L

F

F

Y

P

P

Y

V

F

I

T

P

L

L

F

I

A

A

G

I

L

I

-

G

V

G

L

L

S

Y

I

I

S

I

F

F

V

N

L

T

F

L

L

I

F

V

R

Q

P

P

E

K

N

N

binding alpha-aminoisobutyric acid: V17 (= V27), G19 (= G29), A20 (≠ T30), G21 (= G31), F199 (= F209), A200 (≠ S210), D202 (≠ G212)

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
24% identity, 34% coverage: 188:337/445 of query aligns to 207:356/458 of 6f34A

query
sites
6f34A

N

N

Y

W

Q

T

N

P

F

F

V

M

P

P

N

Y

G

G

W

F

G

S

A

G

V

V

F

A

A

T

S

G

L

A

V

T

V

V

I

F

F
|
F

S
x
A

Y

Y

G
x
I

G

G

S

F

E

D

L

A

I

V

G

S

L

T

T

A

I

A

T

E

E

E

T

V

K

R

D

N

A

P

E

Q

K

R

V

D

L

M

P

P

-

I

G

G

V

I

K

V

S

S

M

L

W

V

R

C

I

T

I

L

L

L

F

Y

Y

I

T

A

L

V

P

S

I

-

L

L

I

V

I

L

C

T

G

G

L

I

I

V

P

P

W

Y

N

E

Q

Q

I

L

D

N

P

V

N

K

N

N

S

-

P

P

F

V

V

A

Q

F

V

A

F

L

S

N

A

Y

A

I

G

H

M
x
Q

Q

D

G

W

A
x
V

S

A

H

G

F

F

I

I

N

S

F

L

V

G

L

A

I

I

T

A

A

G

V

I

L

T

S

T

A
x
V

A

L

N

L

S

V

G

S

I

M

Y

Y

G

G

T

Q

T

T

R

R

M

L

I

F

H

Y

S

A

L

I

S

S

K

R

S

D

D

G

G

L

P

P

K

K

K

V

L

F

A

A

Q

R

V

I

N

S

-

P

K

T

R

R

G

Q

V

V

P

P

I

Y

L

V

S

N

L

T

W

W

V

L

T

T

binding arginine: F228 (= F209), A229 (≠ S210), I231 (≠ G212), V314 (≠ A296)
binding : Q296 (≠ M278), V299 (≠ A281)

Sites not aligning to the query:

binding arginine: 40, 42, 43, 44, 115, 116, 119
binding cholesterol: 201, 202
binding : 28, 30, 31, 34, 178, 179, 186, 187, 190, 194

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
24% identity, 34% coverage: 188:337/445 of query aligns to 205:354/456 of 5oqtA

query
sites
5oqtA

N

N

Y

W

Q

T

N

P

F

F

V

M

P

P

N

Y

G

G

W

F

G

S

A

G

V

V

F

A

A

T

S

G

L

A

V

T

V

V

I

F

F
|
F

S
x
A

Y

Y

G
x
I

G

G

S

F

E

D

L

A

I

V

G

S

L

T

T

A

I

A

T

E

E

E

T

V

K

R

D

N

A

P

E

Q

K

R

V

D

L

M

P

P

-

I

G

G

V

I

K

V

S

S

M

L

W

V

R

C

I

T

I

L

L

L

F

Y

Y

I

T

A

L

V

P

S

I

-

L

L

I

V

I

L

C

T

G

G

L

I

I

V

P

P

W

Y

N

E

Q

Q

I

L

D

N

P

V

N

K

N

N

S

-

P

P

F

V

V

A

Q

F

V

A

F

L

S

N

A

Y

A

I

G

H

M
x
Q

Q

D

G

W

A
x
V

S

A

H

G

F

F

I

I

N

S

F

L

V

G

L

A

I

I

T

A

A

G

V

I

L

T

S

T

A

V

A

L

N

L

S

V

G

M

I

M

Y

Y

G

G

T

Q

T

T

R

R

M

L

I

F

H

Y

S

A

L

I

S

S

K

R

S

D

D

G

G

L

P

P

K

K

K

V

L

F

A

A

Q

R

V

I

N

S

-

P

K

T

R

R

G

Q

V

V

P

P

I

Y

L

V

S

N

L

T

W

W

V

L

T

T

binding alanine: F226 (= F209), A227 (≠ S210), I229 (≠ G212)
binding : Q294 (≠ M278), V297 (≠ A281)

Sites not aligning to the query:

binding alanine: 38, 40, 41, 42
binding : 24, 26, 28, 29, 32, 176, 177, 184, 188, 192

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
22% identity, 53% coverage: 1:235/445 of query aligns to 9:242/461 of P76037

query
sites
P76037

M

I

S

A

A

A

N

Q

Q

P

E

G

Q

K

H

T

E

R

L

L

K

R

K

K

L

S

L

L

S

K

P

L

R

W

H

Q

I

V

R

V

M

M

I

M

A

G

M

L

G

A

G

Y

V

L

I

T

G

P

T

M

G

T

I

V

F

F

K

D

G

T

S

F

A

G

D

I

T

V

I

S

G

G

L

I

A

S

G

D

P

G

G

H

V

V

I

P

F

A

S

S

Y

Y

V

L

F

L

A

A

G

L

L

A

L

G

L

V

L

L

I

F

V

T

M

A

A

I

A

S

L

Y

A

G

E

K

M

L

A

V

I

R

V

Q

Y

F

P

P

-

E

G

A

N

G

N

S

L

A

R

Y

D

T

L

Y

I

A

Q

Q

I

K

A

S

L

I

G

N

Q

P

R

H

F

V

S

G

F

F

V

M

V

V

G

G

W

W

V

S

Y

S

C

L

F

L

M

D

W
x
Y

L

L

T

F

V

L

C

P

V

M

I

I

E

N

I

V

I

L

A

L

A

A

G

K

S

I

L

Y

L

L

Q

S

Y

A

W

L

M

F

P

P

N

E

V

V

P

P

L

P

W

W

S

V

L

W

C

V

L

V

L

T

C

F

S

V

L

A

L

I

V

L

I

T

G

A

I

A

N

N

L

L

N

K

N

S

V

V

K

N

Y

L

F

V

G

A

E

N

I

F

E

N

F

T

W

L

F

F

A

V

G

L

M

V

K

Q

I

I

F

S

V

I

I

M

I

V

V

V

F

F

I

I

I

F

L

L

G

V

A

V

A

Q

L

G

L

L

F

H

-

K

-

G

-

E

-

G

-

V

G

G

I

T

I

V

P

W

S

S

E

L

Q

Q

S

-

T

-

P

P

V

F

L

I

T

S

N

E

Y

N

Q

A

N

H

F

L

V

I

P

P

N

I

G

I

W

T

G

G

A

A

V

T

F

-

A

-

S

-

L

-

V

I

V

V

I

C

F

F

S

S

Y

F

G

L

G

G

S

F

E

D

L

A

I

V

G

T

L

T

T

L

I

S

T

E

E

E

T

T

K

P

D

D

A

A

E

A

K

R

V

V

L

I

P

P

G

K

V

A

V

I

Y110 (≠ W101) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

Query Sequence

>WP_035704030.1 NCBI__GCF_000691145.1:WP_035704030.1
MSANQEQHELKKLLSPRHIRMIAMGGVIGTGIFKGSADTIGLAGPGVIFSYVFAGLLLLI
VMAALAEMAIVYPGNNLRDLIQIALGQRFSFVVGWVYCFMWLTVCVIEIIAAGSLLQYWM
PNVPLWSLCLLCSLLVIGINLNNVKYFGEIEFWFAGMKIFVIIVFIILGAALLFGIIPSE
QSTPVLTNYQNFVPNGWGAVFASLLVVIFSYGGSELIGLTITETKDAEKVLPGVVKSMMW
RIILFYTLPILIICGLIPWNQIDPNNSPFVQVFSAAGMQGASHFINFVLITAVLSAANSG
IYGTTRMIHSLSKSDGGPKKLAQVNKRGVPILSLWVTVLFLLIGTFFAYLYPDQIFSYML
AIPGFAVSLIWISICLAHLKLRPRYPQEPYFKVWLFPYFTLFAGLVLSISFVLFLFRPEN
LPSSIISIGFLAAAIIASFLMKKKA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory