SitesBLAST
Comparing WP_035704417.1 NCBI__GCF_000691145.1:WP_035704417.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
47% identity, 95% coverage: 14:260/260 of query aligns to 15:261/261 of 5jbxB
- active site: A67 (= A66), R72 (= R71), L84 (≠ V83), R88 (≠ Q87), G112 (= G111), E115 (= E114), T134 (≠ P133), E135 (= E134), I140 (= I139), P142 (= P141), G143 (= G142), A228 (≠ T227), L238 (≠ I237)
- binding coenzyme a: S24 (≠ A23), R25 (≠ Q24), R26 (≠ A25), A28 (= A27), A65 (= A64), D68 (= D67), L69 (= L68), K70 (= K69), L110 (= L109), G111 (= G110), T134 (≠ P133), E135 (= E134), L138 (= L137), R168 (≠ S167)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
45% identity, 94% coverage: 17:260/260 of query aligns to 17:259/259 of 5zaiC
- active site: A65 (= A66), F70 (≠ R71), S82 (≠ V83), R86 (≠ Q87), G110 (= G111), E113 (= E114), P132 (= P133), E133 (= E134), I138 (= I139), P140 (= P141), G141 (= G142), A226 (≠ T227), F236 (≠ I237)
- binding coenzyme a: K24 (≠ Q24), L25 (≠ A25), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (≠ L68), P132 (= P133), R166 (≠ S167), F248 (= F249), K251 (= K252)
Q13825 Methylglutaconyl-CoA hydratase, mitochondrial; 3-MG-CoA hydratase; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydratase; Itaconyl-CoA hydratase; EC 4.2.1.18; EC 4.2.1.56 from Homo sapiens (Human) (see 4 papers)
44% identity, 98% coverage: 6:260/260 of query aligns to 81:339/339 of Q13825
- K105 (≠ A30) mutation to N: Abolishes RNA-binding; when associated with E-109 and Q-113.
- 105:119 (vs. 30:44, 7% identical) RNA-binding
- K109 (≠ E34) mutation to E: Abolishes RNA-binding; when associated with N-105 and Q-113.
- K113 (≠ Q38) mutation to Q: Abolishes RNA-binding; when associated with N-105 and E-109.
- A240 (≠ G165) to V: in MGCA1; decreased methylglutaconyl-CoA hydratase activity; dbSNP:rs769894315
Sites not aligning to the query:
- 1:67 modified: transit peptide, Mitochondrion
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
40% identity, 95% coverage: 10:257/260 of query aligns to 9:253/256 of 3h81A
- active site: A64 (= A66), M69 (≠ R71), T79 (≠ E81), F83 (≠ Q87), G107 (= G111), E110 (= E114), P129 (= P133), E130 (= E134), V135 (≠ I139), P137 (= P141), G138 (= G142), L223 (≠ T227), F233 (≠ I237)
- binding calcium ion: F233 (≠ I237), Q238 (≠ R242)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
40% identity, 95% coverage: 10:257/260 of query aligns to 10:254/255 of 3q0jC
- active site: A65 (= A66), M70 (≠ R71), T80 (≠ E81), F84 (≠ Q87), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ T227), F234 (≠ I237)
- binding acetoacetyl-coenzyme a: Q23 (≠ A23), A24 (≠ Q24), L25 (≠ A25), A27 (= A27), A63 (= A64), G64 (= G65), A65 (= A66), D66 (= D67), I67 (≠ L68), K68 (= K69), M70 (≠ R71), F84 (≠ Q87), G107 (= G110), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), P138 (= P141), G139 (= G142), M140 (≠ A143)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
40% identity, 95% coverage: 10:257/260 of query aligns to 10:254/255 of 3q0gC
- active site: A65 (= A66), M70 (≠ R71), T80 (≠ E81), F84 (≠ Q87), G108 (= G111), E111 (= E114), P130 (= P133), E131 (= E134), V136 (≠ I139), P138 (= P141), G139 (= G142), L224 (≠ T227), F234 (≠ I237)
- binding coenzyme a: L25 (≠ A25), A63 (= A64), I67 (≠ L68), K68 (= K69), Y104 (≠ H107), P130 (= P133), E131 (= E134), L134 (= L137)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
37% identity, 96% coverage: 10:258/260 of query aligns to 13:256/258 of 1mj3A
- active site: A68 (= A66), M73 (≠ R71), S83 (≠ E81), L85 (≠ V83), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ T227), F235 (≠ I237)
- binding hexanoyl-coenzyme a: K26 (≠ A23), A27 (≠ Q24), L28 (≠ A25), A30 (= A27), A66 (= A64), G67 (= G65), A68 (= A66), D69 (= D67), I70 (≠ L68), G109 (= G111), P131 (= P133), E132 (= E134), L135 (= L137), G140 (= G142)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
37% identity, 96% coverage: 10:258/260 of query aligns to 13:258/260 of 1dubA
- active site: A68 (= A66), M73 (≠ R71), S83 (≠ E81), L87 (≠ T85), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ T227), F237 (≠ I237)
- binding acetoacetyl-coenzyme a: K26 (≠ A23), A27 (≠ Q24), L28 (≠ A25), A30 (= A27), A66 (= A64), A68 (= A66), D69 (= D67), I70 (≠ L68), Y107 (≠ H107), G110 (= G110), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), L137 (= L137), G142 (= G142), F233 (≠ Y233), F249 (= F249)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
37% identity, 96% coverage: 10:258/260 of query aligns to 11:256/258 of 1ey3A
- active site: A66 (= A66), M71 (≠ R71), S81 (≠ E81), L85 (≠ T85), G109 (= G111), E112 (= E114), P131 (= P133), E132 (= E134), T137 (≠ I139), P139 (= P141), G140 (= G142), K225 (≠ T227), F235 (≠ I237)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ A23), L26 (≠ A25), A28 (= A27), A64 (= A64), G65 (= G65), A66 (= A66), D67 (= D67), I68 (≠ L68), L85 (≠ T85), W88 (≠ F90), G109 (= G111), P131 (= P133), L135 (= L137), G140 (= G142)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
37% identity, 96% coverage: 10:258/260 of query aligns to 43:288/290 of P14604
- E144 (= E114) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E134) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
37% identity, 96% coverage: 10:258/260 of query aligns to 12:252/254 of 2dubA
- active site: A67 (= A66), M72 (≠ R71), S82 (≠ Q88), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), T133 (≠ I139), P135 (= P141), G136 (= G142), K221 (≠ T227), F231 (≠ I237)
- binding octanoyl-coenzyme a: K25 (≠ A23), A26 (≠ Q24), L27 (≠ A25), A29 (= A27), A65 (= A64), A67 (= A66), D68 (= D67), I69 (≠ L68), K70 (= K69), G105 (= G111), E108 (= E114), P127 (= P133), E128 (= E134), G136 (= G142), A137 (= A143)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
39% identity, 95% coverage: 10:257/260 of query aligns to 9:249/250 of 3q0gD
- active site: A64 (= A66), M69 (≠ Q77), T75 (≠ V83), F79 (≠ Q87), G103 (= G111), E106 (= E114), P125 (= P133), E126 (= E134), V131 (≠ I139), P133 (= P141), G134 (= G142), L219 (≠ T227), F229 (≠ I237)
- binding Butyryl Coenzyme A: F225 (≠ Y233), F241 (= F249)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
35% identity, 96% coverage: 10:258/260 of query aligns to 13:258/260 of 2hw5C
- active site: A68 (= A66), M73 (≠ R71), S83 (≠ E81), L87 (≠ T85), G111 (= G111), E114 (= E114), P133 (= P133), E134 (= E134), T139 (≠ I139), P141 (= P141), G142 (= G142), K227 (≠ T227), F237 (≠ I237)
- binding crotonyl coenzyme a: K26 (≠ A23), A27 (≠ Q24), L28 (≠ A25), A30 (= A27), K62 (= K60), I70 (≠ L68), F109 (≠ L109)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
33% identity, 97% coverage: 10:260/260 of query aligns to 6:245/245 of 6slaAAA
- active site: Q61 (≠ A66), L68 (= L73), N72 (≠ Q77), A96 (≠ G111), S99 (≠ E114), A118 (≠ P133), F119 (≠ E134), L124 (≠ I139), P126 (= P141), N127 (≠ G142), A212 (≠ T227), G222 (≠ I237)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ A25), A59 (= A64), Q61 (≠ A66), D62 (= D67), L63 (= L68), L68 (= L73), Y71 (≠ E76), A94 (≠ L109), G95 (= G110), A96 (≠ G111), F119 (≠ E134), I122 (≠ L137), L124 (≠ I139), N127 (≠ G142), F234 (= F249), K237 (= K252)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
33% identity, 97% coverage: 10:260/260 of query aligns to 9:257/257 of 6slbAAA
- active site: Q64 (≠ A66), F69 (≠ L73), L80 (= L84), N84 (≠ Q87), A108 (≠ G111), S111 (≠ E114), A130 (≠ P133), F131 (≠ E134), L136 (≠ I139), P138 (= P141), D139 (≠ G142), A224 (≠ T227), G234 (≠ I237)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K60), A62 (= A64), Q64 (≠ A66), D65 (= D67), L66 (= L68), Y76 (≠ K80), A108 (≠ G111), F131 (≠ E134), D139 (≠ G142)
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
40% identity, 76% coverage: 2:198/260 of query aligns to 1:201/707 of 6yswA
- active site: A66 (= A66), I71 (vs. gap), A84 (≠ V83), Q88 (= Q87), G112 (= G111), E115 (= E114), P136 (= P133), E137 (= E134), G145 (= G142)
- binding coenzyme a: E23 (≠ Q24), M25 (vs. gap), A66 (= A66), D67 (= D67), I68 (≠ L68), P136 (= P133), E137 (= E134), L140 (= L137)
Sites not aligning to the query:
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
31% identity, 97% coverage: 8:258/260 of query aligns to 15:266/270 of Q4WF54