SitesBLAST
Comparing WP_035704441.1 NCBI__GCF_000691145.1:WP_035704441.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 6 hits to proteins with known functional sites (download)
5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
23% identity, 54% coverage: 195:433/446 of query aligns to 2:264/291 of 5odqB
- binding Non-cubane [4Fe-4S]-cluster: G8 (= G201), C9 (= C202), C41 (= C234), C42 (= C235), C78 (≠ A271), G80 (= G273), C81 (= C274), G152 (≠ S332), C153 (= C333), H154 (= H334), C193 (= C366), C194 (= C367), C231 (≠ N404), F233 (≠ G406), C234 (= C407)
- binding 2-bromanylethanesulfonic acid: G46 (= G240), G80 (= G273), H154 (= H334), G197 (≠ A370), R201 (≠ N374), F233 (≠ G406), L236 (= L409)
5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
23% identity, 54% coverage: 195:433/446 of query aligns to 2:264/291 of 5odhH
- binding Non-cubane [4Fe-4S]-cluster: G8 (= G201), C9 (= C202), C41 (= C234), C42 (= C235), C78 (≠ A271), G80 (= G273), C81 (= C274), C153 (= C333), H154 (= H334), C193 (= C366), C194 (= C367), C231 (≠ N404), F233 (≠ G406), C234 (= C407)
- binding 1-thioethanesulfonic acid: A44 (= A237), P45 (≠ H239), G46 (= G240), G80 (= G273), H154 (= H334), G197 (≠ A370), G198 (= G371)
- binding Coenzyme B: R201 (≠ N374), F233 (≠ G406), R240 (≠ A413)
5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
23% identity, 54% coverage: 195:433/446 of query aligns to 2:264/291 of 5odhB
- binding Non-cubane [4Fe-4S]-cluster: G8 (= G201), C9 (= C202), I10 (≠ L203), C41 (= C234), C42 (= C235), C78 (≠ A271), G80 (= G273), C81 (= C274), G152 (≠ S332), C153 (= C333), H154 (= H334), C193 (= C366), C194 (= C367), C231 (≠ N404), F233 (≠ G406), C234 (= C407)
- binding 1-thioethanesulfonic acid: P45 (≠ H239), G46 (= G240), G80 (= G273), F233 (≠ G406)
5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
23% identity, 54% coverage: 195:433/446 of query aligns to 2:264/291 of 5odcB
- binding Non-cubane [4Fe-4S]-cluster: G8 (= G201), C9 (= C202), I10 (≠ L203), C41 (= C234), C42 (= C235), C78 (≠ A271), N79 (≠ G272), G80 (= G273), C81 (= C274), G152 (≠ S332), C153 (= C333), C193 (= C366), C194 (= C367), G197 (≠ A370), C231 (≠ N404), F233 (≠ G406), C234 (= C407)
P21912 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; Malate dehydrogenase [quinone] iron-sulfur subunit; EC 1.3.5.1; EC 1.1.5.- from Homo sapiens (Human) (see 10 papers)
24% identity, 26% coverage: 1:115/446 of query aligns to 157:280/280 of P21912
- S163 (≠ Q7) to P: found in a patient with a Cowden-like phenotype; uncertain significance; patient cells have increased manganese superoxide dismutase expression and increased levels of reactive oxygen species; 2.7-fold increase in AKT expression and a 1.7-fold increase in MAPK expression; dbSNP:rs33927012
- C186 (= C26) binding [4Fe-4S] cluster
- C189 (= C29) binding [4Fe-4S] cluster
- C192 (= C32) binding [4Fe-4S] cluster
- C196 (= C36) binding [3Fe-4S] cluster
- W201 (≠ E41) binding a ubiquinone
- C243 (= C78) binding [3Fe-4S] cluster
- C249 (= C84) binding [3Fe-4S] cluster
- C253 (= C88) binding [4Fe-4S] cluster
- L257 (≠ V92) to V: in MC2DN4; decreased succinate dehydrogenase (ubiquinone) activity; decreased protein levels in homozygous patient cells; dbSNP:rs761350633
Sites not aligning to the query:
- 3 A → G: found in a patient with a Cowden-like phenotype; uncertain significance; patient cells have increased manganese superoxide dismutase expression and normal levels of reactive oxygen species; 1.2-fold increase in AKT expression and 1.3-fold change in MAPK expression in patient cells; dbSNP:rs11203289
- 40 natural variant: K -> E
- 48 D → V: in MC2DN4; decreased succinate dehydrogenase (ubiquinone) activity in homozygous patient cells; decreased protein levels in homozygous patient cells; dbSNP:rs202101384
- 93 binding [2Fe-2S] cluster
- 98 binding [2Fe-2S] cluster
- 100 S → F: in PPGL4; absence of expression in tumor cells indicating complete loss of SDHB function; dbSNP:rs121917755
- 101 binding [2Fe-2S] cluster
- 102 A → T: in MC2DN4; uncertain significance; dbSNP:rs777578399
- 113 binding [2Fe-2S] cluster
- 146:218 Interaction with SDHAF1
Q007T0 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; Iron-sulfur subunit of complex II; Ip; Malate dehydrogenase [quinone] iron-sulfur subunit; EC 1.3.5.1; EC 1.1.5.- from Sus scrofa (Pig) (see paper)
24% identity, 25% coverage: 1:111/446 of query aligns to 157:276/280 of Q007T0
- C186 (= C26) binding [4Fe-4S] cluster
- C189 (= C29) binding [4Fe-4S] cluster
- C192 (= C32) binding [4Fe-4S] cluster
- C196 (= C36) binding [3Fe-4S] cluster
- W201 (≠ E41) binding a ubiquinone
- C243 (= C78) binding [3Fe-4S] cluster
- C249 (= C84) binding [3Fe-4S] cluster
- C253 (= C88) binding [4Fe-4S] cluster
Sites not aligning to the query:
- 93 binding [2Fe-2S] cluster
- 98 binding [2Fe-2S] cluster
- 101 binding [2Fe-2S] cluster
- 113 binding [2Fe-2S] cluster
Query Sequence
>WP_035704441.1 NCBI__GCF_000691145.1:WP_035704441.1
MSSSSKQKEIQQQFQEQMDEKELLNCMRCGFCLPSCPTYIESGQQETHSPRGRIALMKAV
RDGVIEPDEDVEHSLNLCLGCRACEPVCPSGVKYGQLLEDARDIIQQHKKQSFPVKLMRR
VVFKGLFPSQSRMRQAASFLRFYQTSGLQTAARKTRILKVLPSHLQLMEQALPKVPPQKK
KRLNEYKAIGQAKKRVAFFTGCLMDTVFSNTNKATIHLLQLAGCDVIVPPIQTCCGALHG
HSGEKDQAKQLAKRNIEAFEETDADAIIMNAGGCGAFLSDYGHLLQDDPDFQKRSKAFSQ
KITDISSILVELEFHCRVPLALPAQVITYQDSCHLRNGMNVQQAPRVLMKAIQGVTFKEM
KDAGRCCGSAGIYNILQPDMSMQILDHKMTEASNTAAAAIVTSNPGCQLQMAAGVKKAGH
MPSMRAIHLADFLLEAVQYGKKASSS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory