SitesBLAST

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
25% identity, 45% coverage: 25:256/510 of query aligns to 56:269/444 of Q8NLB7

query
sites
Q8NLB7

Y

Y

D
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D

F

L

Y

I

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V

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Y

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N

N

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A

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R

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L

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D

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I
x
R

I

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G

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R

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K

Y

A

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Y

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S

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V

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K

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A

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H

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D

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R

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E

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N

A

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K

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D

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Y

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R

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A

A

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G

-

A

A

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M

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G

L

V

L

V

A

W

W

Y

Y

D57 (= D26) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (≠ I78) mutation to A: Loss of transport activity.

Sites not aligning to the query:

54 D→A: Loss of transport activity.; D→E: Retains 50% of its transport activity.
309 W→V: Loss of transport activity.
312 D→A: Loss of transport activity.
313 R→A: Loss of transport activity.
317 mutation I->H,Y: Loss of transport activity.
386 R→A: Loss of transport activity.

P36035 Carboxylic acid transporter protein homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 30% coverage: 45:196/510 of query aligns to 171:305/616 of P36035

query
sites
P36035

D

D

N

R

P

P

T

T

A

K

A

D

F

I

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-

A

-

T

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G

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L

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C

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W

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S

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L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
9 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
338 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

P25297 Inorganic phosphate transporter PHO84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 6 papers)
24% identity, 40% coverage: 11:213/510 of query aligns to 64:276/587 of P25297

query
sites
P25297

K

K

V

T

I

I

S

S

A

I

S

A

S

G

M

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G

G

T

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M

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S

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Y

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D

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F

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A

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S

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L

G

A

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M

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M

S

S

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Y

K

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F

Y

F

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P

H

A

G

D

S

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M

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P

T

G

A

P

A

S

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T

A

L

T

L

L

K

A

V

T

S

F

T

A

S

V

V

G

G

F

T

V

V

V

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R

G

P

Q

F

F

G

G

A

-

L

-

F

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F

F

G

G

R

T

L

L

G

A

D

D

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I

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G

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K

Y

R

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Y

M

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L

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M

G

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G

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A
x
C

T

T

F

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L

I

Q

G

T

C

T

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P

A

S

H

Y

S

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P

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A

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I

G

N

F
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F

M

V

A

A

P

-

L

-

L

V

V

L

L

T

I

F

L

Y

R
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R

M

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L

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M

G
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G

L

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A
x
G

L

I

G
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G

G

G

E
x
D

Y
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Y

G

P

G

L

A

S

T

I

Y

I

V

T

A

S

E

E

H

F

A

A

P

T

V

T

G

K

Q

W

R

R

G

G

Y

A

W

I

-

M

-

G

-

A

-

V

-

F

-

A

-

N

T

Q

S

A

W

W

I

G

Q

Q

T

I

T

S

A

-

T

-

V

-

G

G

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G

F

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I

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S

A

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M

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I

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A

A

A

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K

N

G

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P

-

E

-

E

E

F

Y

D

A

N

N

W

S

G

G

-

A

-

E

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x
C

-

D

-

A

-

R

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x
C

-

Q

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K

-

A

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x
C

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D

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Q

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M

W

W

R

R

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L

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G

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G

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G

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S
x
C

Y

L

L

Y

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F

R

R

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M

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D

P

E

E

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S

P

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F

Y

C145 (≠ A95) mutation to S: No significant effect on transport activity; when associated with S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
F160 (= F110) mutation to C: Reduces phosphate binding, transport and signaling activities.
R168 (= R120) mutation to A: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; mutation to C: Abolishes transport and signaling activities.; mutation to E: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. No significant effects on growth rates.; mutation to Q: Reduces transport activity. Moderately reduces affinity for inorganic phosphate. Moderately reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
G172 (= G124) mutation to C: Abolishes transport and signaling activities.
G174 (≠ A126) mutation to C: Abolishes transport and signaling activities.
G176 (= G128) mutation to C: Abolishes transport and signaling activities.
D178 (≠ E130) mutation D->E,N: Reduces transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on signaling activities. No significant effects on growth rates.
Y179 (= Y131) mutation to A: Significantly reduces transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Slightly reduces signaling activity.; mutation to G: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Reduces growth rates under low phosphate conditions. Abolishes signaling activity.; mutation Y->S,F: No significant effects on transport activity. No significant effects on growth rates under low phosphate conditions. Does not affect patterns of secreted phosphatase activities under low and high phosphate conditions. No significant effects on signaling activity.
C237 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C241 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-245, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C245 (vs. gap) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-263, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.
C263 (≠ S200) mutation to S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-335, S-399, S-434, S-455, S-474, S-510 and S-519.

Sites not aligning to the query:

6 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
298 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); K→A: No significant effect on membrane localization under low phosphate conditions and internalization after phosphate addition. Strongly affects vacuolar sorting of the protein after internalization.
304:327 mutation Missing: Increases transport activity. Has no significant effect on membrane localization under low phosphate conditions. Results in severely delayed internalization after phosphate addition.
335 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-399, S-434, S-455, S-474, S-510 and S-519.
358 D→E: Abolishes transport activity. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. Moderately decreases signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.; D→N: Significantly reduces transport activity. No significant effects on affinity for inorganic phosphate. Causes pronounced increase in secreted phosphatase activity under high phosphate conditions. No significant effects on signaling activities. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
392 V→C: Reduces transport and signaling activities.
399 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-434, S-455, S-474, S-510 and S-519.
408 V→C: Reduces signaling activity and enhances transport activity.
434 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-455, S-474, S-510 and S-519.
455 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-474, S-510 and S-519.
473 E→K: No significant effects on transport activity. No significant effects on affinity for inorganic phosphate. No significant effects on growth rates.; E→Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.
474 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-510 and S-519.
492 mutation K->A,Q: No significant effects on transport activity. Reduces affinity for inorganic phosphate. No significant effects on growth rates.; K→E: Reduces transport activity. Reduces affinity for inorganic phosphate. Significantly reduces growth rates under low phosphate conditions. No significant effects on growth rates under high phosphate conditions.
510 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-519.
519 C→S: No significant effect on transport activity; when associated with S-145, S-237, S-241, S-245, S-263, S-335, S-399, S-434, S-455, S-474 and S-510.

O42885 Putative inorganic phosphate transporter C8E4.01c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
27% identity, 30% coverage: 67:221/510 of query aligns to 108:273/572 of O42885

query
sites
O42885

F

F

G

G

A

Q

L

L

F

L

F

F

G

G

R

F

L

M

G

G

D

D

I

F

G

G

R

R

K

K

Y

F

T

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F

Y

M

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K

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A

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Y

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A

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E

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K

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R

R

R

G

G

Y

T

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S

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I

I

-

F

-

A

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F

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Q

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T

F

A

G

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-

A

G

G

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A

F

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L

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G

A

C

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N

H

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L

L

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N

P

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E

E

E

G

F

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Y

N

H

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K

-

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G

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W

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F

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S

F

Y

A

E

K

N

A

S

K

K

-

A

-

L

-

N

-

S

S

A

E

E

G

G

K

K

Sites not aligning to the query:

12 modified: Phosphoserine
14 modified: Phosphoserine
292 modified: Phosphoserine
296 modified: Phosphoserine

Q09852 Putative inorganic phosphate transporter C23D3.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 31% coverage: 67:225/510 of query aligns to 103:268/559 of Q09852

query
sites
Q09852

F

F

G

G

A

Q

L

I

F

L

F

F

G

G

R

F

L

M

G

G

D

D

I

F

G

G

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R

K

K

Y

F

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Y

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A

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E

H

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A

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G

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S

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Q

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G

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G

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G

A

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G

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A

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L

L

A

A

T

C

-

F

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E

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K

-

P

-

L

-

N

-

Q

R

R

N

G

V

E

L

Y

T

T

P

K

E

L

E

E

F

G

D

V

N

-

W

-

G

-

W

W

R

R

V

L

P

Q

F

M

W

G

V

L

S

A

I

L

V

V

M

P

V

A

G

L

I

L

S

V

Y

L

L

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R

K

L

N

T

M

M

D

K

E

E

S

S

P

K

V

S

F

Y

A

E

K

Q

A

S

K

K

S

A

E

L

G

N

K

K

T

Y

S

T

T

D

N

N

Sites not aligning to the query:

297 modified: Phosphoserine
299 modified: Phosphoserine

Query Sequence

>WP_035717838.1 NCBI__GCF_002017945.1:WP_035717838.1
MSNKSTKGIWKVISASSMGTMIEWYDFYIFGSLAVVISTKFFPADNPTAAFLATLATFAV
GFVVRPFGALFFGRLGDIIGRKYTFMVTLLLMGGATFLIGCIPSYETIGFMAPLLVLILR
MLQGLALGGEYGGAATYVAEHAPVGQRGYWTSWIQTTATVGLFISLMVILATRNVLTPEE
FDNWGWRVPFWVSIVMVGISYLIRKNMDESPVFAKAKSEGKTSTNPLKESFGNRYNLKFV
LLALFGATMGQGVVWYTGQFYAMSFLKTVMTIDSSQVDQLLGIALILGTPFFIFFGWLSD
KLGRKYIMMGGMLIAILLYRPIYQAMFETTNIKNKTELVDQTTIVAETKTNKQALDSIYT
TTKVFEDGTKWVEIKTIPLENGQAKIVDGKPKADIKTTLTINNTDMWMLVFYIFVQVLFV
TMVYGPIAAFLVEMFPVKIRYTSMSLPYHVGNGIFGGLLPAISTYLVTNAKEAGDVNYYL
EGLWYPIIIASVCFVIGMIYLDNSKTNKHV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory