SitesBLAST

P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
34% identity, 79% coverage: 17:97/103 of query aligns to 8:89/168 of P9WKJ3

query
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P9WKJ3

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K44 (≠ S53) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

A0QUX7 Acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
33% identity, 79% coverage: 17:97/103 of query aligns to 10:91/170 of A0QUX7

query
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A0QUX7

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K46 (≠ S53) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 81% coverage: 16:98/103 of query aligns to 309:390/477 of Q9FFF4

query
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Q9FFF4

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Sites not aligning to the query:

88 G→D: Resistance to valine ihibition.
119 T→I: In vat1-1; resistance to valine ihibition.
453 R→Q: In vat1-3; resistance to valine ihibition.

Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 92% coverage: 4:98/103 of query aligns to 309:402/491 of Q93YZ7

query
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Q93YZ7

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D328 (≠ N23) binding
G331 (= G26) mutation to D: Decreased feedback inhibition by leucine and valine/isoleucine.
V332 (= V27) binding
L333 (≠ M28) binding
F340 (= F35) mutation to A: Decreased feedback inhibition by valine/isoleucine.
Y345 (= Y40) mutation to A: No effect.
N346 (= N41) binding ; mutation N->A,H: Decreased feedback inhibition by leucine and increased feedback inhibition by valine/isoleucine.
I347 (≠ V42) binding

Sites not aligning to the query:

95 binding
98 G→E: Increased feedback inhibition by valine/isoleucine.
99 binding
100 binding
107 F→A: Decreased feedback inhibition by leucine and valine/isoleucine.
112 Y→A: Increased feedback inhibition by leucine.
113 binding ; N→A: Decreased feedback inhibition by valine/isoleucine.
114 binding

6vz8G Arabidopsis thaliana acetohydroxyacid synthase complex with valine bound (see paper)
31% identity, 80% coverage: 17:98/103 of query aligns to 6:86/159 of 6vz8G

query
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6vz8G

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binding valine: D12 (≠ N23), G15 (= G26), V16 (= V27), L17 (≠ M28), N30 (= N41), I31 (≠ V42), V36 (≠ C47)

Query Sequence

>WP_035854753.1 NCBI__GCF_000519045.1:WP_035854753.1
MNALSQTERQTLSRAVLEIDVNNHAGVMSHVVGLFSRRAYNVEGILCLPLADSGQSRIWL
LVNEDQRLPQMIKQVEKLEDVAAIRRHNADHAVFQNLEAFFRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory