SitesBLAST
Comparing WP_035946450.1 NCBI__GCF_000768675.1:WP_035946450.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
30% identity, 88% coverage: 11:414/457 of query aligns to 5:421/425 of O59010
- S65 (≠ T65) mutation to V: Strongly decreased chloride conductance.
- R276 (≠ S274) mutation to S: Increased rate of aspartate transport; when associated with R-395.
- RSS 276:278 (≠ SSS 274:276) binding L-aspartate
- M311 (≠ L309) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
- T314 (= T312) binding L-aspartate
- V355 (= V353) binding L-aspartate
- D394 (≠ S387) binding L-aspartate
- M395 (≠ E388) mutation to R: Increased rate of aspartate transport; when associated with S-276.
- R397 (= R390) mutation to A: Strongly decreased affinity for aspartate.
- N401 (= N394) binding L-aspartate
- D405 (≠ N398) mutation to N: Strongly decreased affinity for aspartate.
2nwwA Crystal structure of gltph in complex with tboa (see paper)
30% identity, 84% coverage: 22:405/457 of query aligns to 7:403/407 of 2nwwA
6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
30% identity, 86% coverage: 22:412/457 of query aligns to 16:419/419 of 6x15A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: F46 (≠ L46), F46 (≠ L46), P75 (≠ A76), L91 (≠ T92), F95 (≠ V96), L130 (≠ T135), I133 (≠ F138), I159 (≠ L164), Y167 (vs. gap), K196 (≠ R193), G200 (≠ M197), I207 (= I204), F210 (= F207), L250 (= L247), I262 (≠ L260), M269 (≠ F267), T334 (≠ S332), V335 (≠ I333), G336 (= G334), T340 (≠ S338), L343 (= L341), M399 (≠ L392)
- binding aspartic acid: S277 (= S275), S278 (= S276), T314 (= T312), G354 (= G352), A358 (= A356), G359 (= G357), D394 (≠ S387), R397 (= R390), T398 (≠ A391)
- binding sodium ion: Y89 (≠ F90), T92 (≠ M93), S93 (= S94), G306 (= G304), T308 (≠ S306), N310 (= N308), N310 (= N308), M311 (≠ L309), D312 (= D310), S349 (= S347), I350 (≠ K348), T352 (≠ A350), N401 (= N394), V402 (≠ F395), D405 (≠ N398)
Sites not aligning to the query:
6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
30% identity, 84% coverage: 22:405/457 of query aligns to 13:409/413 of 6x14A
- binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: G66 (= G69), V83 (≠ L87), I157 (≠ L165), Y164 (vs. gap), K193 (≠ R193), T305 (≠ S306), I306 (≠ F307), I347 (≠ K348)
- binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ V22), M199 (= M199), S275 (= S276), T311 (= T312), G356 (= G357), L384 (≠ V380), D391 (≠ S387), R394 (= R390)
Sites not aligning to the query:
6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
30% identity, 85% coverage: 22:410/457 of query aligns to 8:409/409 of 6bavA
6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
30% identity, 84% coverage: 22:407/457 of query aligns to 8:406/408 of 6bauA
- binding cysteine: S270 (= S276), M303 (≠ L309), T306 (= T312), A345 (= A351), G346 (= G352), V347 (= V353), G351 (= G357), D386 (≠ S387), C389 (≠ R390), T390 (≠ A391), N393 (= N394)
6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
31% identity, 89% coverage: 11:416/457 of query aligns to 1:421/425 of 6zgbA
6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
31% identity, 89% coverage: 10:416/457 of query aligns to 2:423/426 of 6xwnB
5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
31% identity, 89% coverage: 10:416/457 of query aligns to 2:423/427 of 5e9sA
- binding aspartic acid: R274 (≠ S274), S275 (= S275), S276 (= S276), T313 (= T312), G353 (= G352), V354 (= V353), A357 (= A356), G358 (= G357), D394 (≠ S387), R397 (= R390), T398 (≠ A391)
- binding decyl-beta-d-maltopyranoside: L194 (≠ R193), G198 (≠ M197), Y202 (≠ A201)
- binding sodium ion: Y87 (≠ F90), T90 (≠ M93), S91 (= S94), S276 (= S276), G305 (= G304), A306 (≠ Y305), T307 (≠ S306), N309 (= N308), N309 (= N308), M310 (≠ L309), D311 (= D310), S348 (= S347), I349 (≠ K348), G350 (= G349), T351 (≠ A350), N401 (= N394), V402 (≠ F395), D405 (≠ N398)
6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
31% identity, 86% coverage: 26:416/457 of query aligns to 15:420/424 of 6zl4A
- binding decyl-beta-d-maltopyranoside: L191 (≠ R193), G195 (≠ M197), R282 (≠ I284)
- binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ S274), S272 (= S275), S273 (= S276), M307 (≠ L309), T310 (= T312), G353 (= G355), A354 (= A356), R394 (= R390), T395 (≠ A391)
Sites not aligning to the query:
6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
31% identity, 86% coverage: 26:416/457 of query aligns to 11:412/416 of 6r7rA