SitesBLAST
Comparing WP_036133695.1 NCBI__GCF_000768355.1:WP_036133695.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
31% identity, 37% coverage: 3:293/791 of query aligns to 23:311/314 of P00348
- GGGLMG 34:39 (≠ GAGVMG 14:19) binding NAD(+)
- D57 (= D37) binding NAD(+)
- E122 (= E97) binding NAD(+)
- K127 (= K102) binding NAD(+)
- S149 (= S124) binding NAD(+)
1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
31% identity, 37% coverage: 4:293/791 of query aligns to 1:288/291 of 1f0yA
- active site: S126 (= S124), H147 (= H147), E159 (= E159), N197 (= N198)
- binding acetoacetyl-coenzyme a: S50 (≠ V49), K57 (≠ L56), S126 (= S124), H147 (= H147), F149 (= F149), N150 (= N150), P151 (= P151), P153 (≠ R153), V154 (≠ Y154), N197 (= N198), P232 (= P233), M233 (≠ S235), L238 (≠ T240)
- binding nicotinamide-adenine-dinucleotide: G13 (= G16), L14 (≠ V17), M15 (= M18), D34 (= D37), Q35 (vs. gap), A96 (= A94), I97 (= I95), E99 (= E97), K104 (= K102), N124 (= N122), S126 (= S124), H147 (= H147), N150 (= N150), V242 (= V244), T246 (= T248), K282 (= K287)
1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
31% identity, 37% coverage: 4:293/791 of query aligns to 1:288/293 of 1f17A
- active site: S126 (= S124), H147 (= H147), E159 (= E159), N197 (= N198)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G16), L14 (≠ V17), M15 (= M18), D34 (= D37), Q35 (vs. gap), A96 (= A94), I97 (= I95), E99 (= E97), K104 (= K102), N124 (= N122), S126 (= S124), H147 (= H147)
1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
31% identity, 37% coverage: 4:293/791 of query aligns to 1:288/293 of 1f12A
- active site: S126 (= S124), H147 (= H147), E159 (= E159), N197 (= N198)
- binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ L56), S126 (= S124), H147 (= H147), F149 (= F149), N150 (= N150), M155 (= M155), N197 (= N198), L200 (≠ G201), P232 (= P233), M233 (≠ S235), L238 (≠ T240)
Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
30% identity, 37% coverage: 4:293/791 of query aligns to 24:311/314 of Q16836
- GGGLMG 34:39 (≠ GAGVMG 14:19) binding NAD(+)
- A40 (= A20) to T: in HADH deficiency; dbSNP:rs137853101
- D57 (= D37) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
- S73 (= S60) binding CoA
- K80 (= K67) binding CoA
- L86 (≠ I73) to P: in dbSNP:rs4956145
- E122 (= E97) binding NAD(+)
- K127 (= K102) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
- S149 (= S124) binding CoA; binding NAD(+)
- Q152 (≠ G127) to H: in dbSNP:rs1051519
- N173 (= N150) binding NAD(+)
- Y226 (≠ S204) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
- P258 (≠ T237) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
- K305 (= K287) binding NAD(+)
P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 36% coverage: 7:294/791 of query aligns to 5:285/286 of P9WNP7
- S122 (= S124) mutation to A: Loss of fatty acyl dehydrogenase activity.
4pzeA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with acetoacetyl-coa (see paper)
32% identity, 36% coverage: 7:293/791 of query aligns to 2:279/283 of 4pzeA
4pzdA Crystal structure of (s)-3-hydroxybutyryl-coa dehydrogenase paah1 in complex with NAD+ (see paper)
32% identity, 36% coverage: 7:293/791 of query aligns to 2:279/283 of 4pzdA
- active site: S118 (= S124), H139 (= H147), E151 (= E159), N189 (= N198)
- binding nicotinamide-adenine-dinucleotide: G11 (= G16), T12 (≠ V17), M13 (= M18), D32 (= D37), A88 (= A94), A89 (≠ I95), T90 (≠ A96), E91 (= E97), I99 (≠ L105), N116 (= N122), S118 (= S124), N142 (= N150)
4kuhA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with acetoacetyl-coa from clostridium butyricum
28% identity, 36% coverage: 7:293/791 of query aligns to 1:278/280 of 4kuhA
4kugA Crystal structure of 3-hydroxybutylryl-coa dehydrogenase with NAD from clostridium butyricum
28% identity, 36% coverage: 7:293/791 of query aligns to 1:278/282 of 4kugA
- active site: S117 (= S124), H138 (= H147), E150 (= E159), N188 (= N198)
- binding nicotinamide-adenine-dinucleotide: G10 (= G16), T11 (≠ V17), M12 (= M18), R30 (≠ F36), D31 (= D37), A87 (= A94), A88 (≠ I95), E90 (= E97), N115 (= N122), S117 (= S124), H138 (= H147)
6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
26% identity, 54% coverage: 7:431/791 of query aligns to 291:692/692 of 6iunB
- active site: S407 (= S124), H428 (= H147), E440 (= E159), N478 (= N198)
- binding nicotinamide-adenine-dinucleotide: G300 (= G16), T301 (≠ V17), M302 (= M18), E321 (≠ D37), T322 (≠ L38), Y365 (≠ L82), A377 (= A94), V378 (≠ I95), E380 (= E97), V384 (≠ W101), V388 (≠ L105), N405 (= N122), S407 (= S124)
Sites not aligning to the query:
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
31% identity, 36% coverage: 7:293/791 of query aligns to 361:637/763 of P40939
- E510 (= E159) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
Sites not aligning to the query:
- 282 V → D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- 305 I → N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- 342 L → P: in LCHAD deficiency; dbSNP:rs137852772
6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
27% identity, 36% coverage: 8:293/791 of query aligns to 1:277/281 of 6aa8E
- active site: S116 (= S124), H137 (= H147), E149 (= E159), N187 (= N198)
- binding nicotinamide-adenine-dinucleotide: I6 (≠ L13), G9 (= G16), T10 (≠ V17), M11 (= M18), R29 (≠ F36), D30 (= D37), I31 (≠ L38), A86 (= A94), A87 (≠ I95), E89 (= E97), K94 (= K102), N114 (= N122), S116 (= S124)
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 38% coverage: 88:388/791 of query aligns to 393:667/729 of P21177
- H450 (= H147) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
Sites not aligning to the query:
- 116 G→F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- 322 G→A: 10-fold increase in KM for NADH.
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
28% identity, 38% coverage: 88:388/791 of query aligns to 393:667/719 of 6tnmA