SitesBLAST
Comparing WP_036136362.1 NCBI__GCF_000768355.1:WP_036136362.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
51% identity, 99% coverage: 4:488/491 of query aligns to 2:458/458 of 6f34A
- binding arginine: I40 (= I50), G42 (= G52), T43 (≠ A53), G44 (= G54), E115 (= E125), Y116 (= Y126), A119 (= A129), F228 (= F257), A229 (= A258), I231 (= I260), V314 (= V344)
- binding cholesterol: W201 (≠ Q223), Y202 (= Y224)
- binding : G28 (≠ T38), F30 (≠ R40), D31 (≠ H41), M34 (= M44), A178 (= A200), R179 (≠ W201), A186 (= A208), I187 (= I209), A190 (≠ T212), L194 (≠ A216), Q296 (≠ L326), V299 (≠ L329)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
51% identity, 99% coverage: 5:488/491 of query aligns to 1:456/456 of 5oqtA
- binding alanine: I38 (= I50), G40 (= G52), T41 (≠ A53), G42 (= G54), F226 (= F257), A227 (= A258), I229 (= I260)
- binding : E24 (≠ V36), G26 (≠ T38), F28 (≠ R40), D29 (≠ H41), M32 (= M44), A176 (= A200), R177 (≠ W201), A184 (= A208), A188 (≠ T212), L192 (≠ A216), Q294 (≠ L326), V297 (≠ L329)
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
38% identity, 82% coverage: 28:431/491 of query aligns to 23:439/629 of P30825
- N226 (≠ G240) modified: carbohydrate, N-linked (GlcNAc...) asparagine
P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
25% identity, 83% coverage: 29:436/491 of query aligns to 14:396/461 of P76037
- Y110 (= Y126) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.
6f2wA Bacterial asc transporter crystal structure in open to in conformation (see paper)
25% identity, 88% coverage: 47:478/491 of query aligns to 14:425/433 of 6f2wA
P82251 b(0,+)-type amino acid transporter 1; b(0,+)AT1; Glycoprotein-associated amino acid transporter b0,+AT1; Solute carrier family 7 member 9 from Homo sapiens (Human) (see 11 papers)
22% identity, 93% coverage: 9:466/491 of query aligns to 8:438/487 of P82251
- V40 (= V47) to M: in CSNU; uncertain significance
- IIGSG 43:47 (≠ IIGAG 50:54) binding L-arginine
- I44 (= I51) to T: in CSNU; type I; dbSNP:rs121908485
- S51 (≠ M58) to F: in CSNU; uncertain significance
- P52 (≠ T59) to L: in CSNU; impairs protein stability and dimer formation; dbSNP:rs1198613438
- A70 (≠ V76) to V: in CSNU; partial loss of amino acid transport activity; dbSNP:rs769448665
- Y99 (= Y105) to H: in CSNU; uncertain significance
- G105 (= G111) to R: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908480
- W114 (= W120) to R: in CSNU; uncertain significance
- I120 (≠ E125) to L: in CSNU; uncertain significance
- T123 (≠ F128) to M: in CSNU; partial loss of amino acid transport activity; dbSNP:rs79987078
- V142 (≠ Y163) to A: no effect on amino acid transport activity; dbSNP:rs12150889
- C144 (≠ A165) modified: Interchain (with C-114 in SLC3A1)
- V170 (≠ Y193) to M: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs121908479
- A182 (≠ N203) to T: in CSNU; type III; partial loss of amino acid transport activity; dbSNP:rs79389353
- G195 (≠ F217) to R: in CSNU; type III; decreased amino acid transport activity; dbSNP:rs121908482
- L223 (≠ V254) to M: slightly decreased amino acid transport activity; dbSNP:rs1007160
- A224 (≠ V255) to V: in CSNU; non-classic type I; dbSNP:rs140873167
- N227 (vs. gap) to D: in CSNU; decreased amino acid transport activity
- W230 (vs. gap) to R: in CSNU; complete loss of amino acid transport activity; mutation to A: Abolishes amino acid transport activity.
- D233 (≠ I260) binding L-arginine; mutation to A: Complete loss of amino acid transport activity.
- W235 (≠ F262) mutation to A: Complete loss of amino acid transport activity.
- Q237 (≠ A264) mutation to A: Reduces amino acid transport activity.
- G259 (≠ S286) to R: in CSNU; type III; impairs protein stability and dimer formation; dbSNP:rs121908483
- P261 (≠ A288) to L: in CSNU; types I and III; dbSNP:rs121908486
- S286 (≠ A313) to F: in CSNU; uncertain significance; dbSNP:rs755135545
- C321 (≠ I345) mutation to S: Does not affect amino acid transport activity.
- A324 (≠ Q352) to E: in CSNU; uncertain significance
- V330 (≠ A358) to M: in CSNU; type III; dbSNP:rs201618022
- A331 (≠ M359) to V: in CSNU; non-classic type I; dbSNP:rs768466784
- R333 (≠ K361) to Q: in CSNU; decreased amino acid transport activity; dbSNP:rs769576205; to W: in CSNU; severe loss of amino acid transport activity; dbSNP:rs121908484
- A354 (≠ G382) to T: in CSNU; type III; severe loss of amino acid transport activity; dbSNP:rs939028046
- S379 (= S407) mutation to A: Markedly reduces amino acid transport activity.
- A382 (≠ T410) to T: in CSNU; severe loss of amino acid transport activity; dbSNP:rs774878350
- W383 (≠ L411) mutation to A: Complete loss of amino acid transport activity.
- Y386 (≠ F414) mutation to A: Loss of amino acid transport activity.
- K401 (≠ P429) to E: in CSNU; uncertain significance; dbSNP:rs760264924
- L426 (≠ W456) to P: in CSNU; uncertain significance
Sites not aligning to the query:
- 482 P → L: in CSNU; severe loss of amino acid transport activity; no effect on localization to the apical membrane; dbSNP:rs146815072; mutation P->A,G,S,V: No effect on amino acid transport activity.; mutation P->F,I,M,W: Decreased amino acid transport activity.
6li9B Heteromeric amino acid transporter b0,+at-rbat complex bound with arginine (see paper)
22% identity, 86% coverage: 47:466/491 of query aligns to 11:409/458 of 6li9B
O34739 Serine/threonine exchanger SteT from Bacillus subtilis (strain 168) (see paper)
24% identity, 85% coverage: 24:438/491 of query aligns to 5:387/438 of O34739
- C94 (≠ I118) mutation to S: Retains 25% of the transport activity; when associated with S-141; S-168; S-291 and S-415.
- C141 (≠ A186) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-168; S-291 and S-415.
- C168 (≠ T212) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-291 and S-415.
- C291 (≠ V344) mutation to S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-415.
Sites not aligning to the query:
- 415 C→S: Retains 25% of the transport activity; when associated with S-94; S-141; S-168 and S-291.
6irtB Human lat1-4f2hc complex bound with bch (see paper)
27% identity, 50% coverage: 240:485/491 of query aligns to 185:431/457 of 6irtB
Sites not aligning to the query:
Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
27% identity, 50% coverage: 240:485/491 of query aligns to 235:481/507 of Q01650
- A246 (≠ G251) mutation to V: Nearly abolishes leucine transport activity.
- F252 (= F257) mutation to A: Nearly abolishes leucine transport activity.
- W257 (≠ F262) mutation to A: Nearly abolishes leucine transport activity.
- N258 (≠ D263) mutation to A: Decreased leucine transport activity.; mutation to D: Nearly abolishes leucine transport activity.
- Y259 (≠ A264) mutation to A: Strongly decreased leucine transport activity.
- E303 (≠ P308) mutation to K: Decreased leucine transport activity.
- P375 (≠ V381) mutation to L: Nearly abolishes leucine transport activity.
Sites not aligning to the query:
- 117 Y→A: Strongly decreased leucine transport activity.
- 164 modified: Interchain (with C-210 in SLC3A2)
- 223 D → V: in dbSNP:rs17853937
- 230 N → K: in dbSNP:rs1060250
- 483:507 mutation Missing: Nearly abolishes leucine transport activity.
8xpuB Overall structure of the lat1-4f2hc bound with jph203 (see paper)
27% identity, 50% coverage: 240:485/491 of query aligns to 192:438/464 of 8xpuB
Sites not aligning to the query:
8idaB Overall structure of the lat1-4f2hc bound with tyrosine
27% identity, 50% coverage: 240:485/491 of query aligns to 192:438/464 of 8idaB