SitesBLAST
Comparing WP_036136365.1 NCBI__GCF_000768355.1:WP_036136365.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
48% identity, 97% coverage: 15:473/474 of query aligns to 13:456/456 of 5oqtA
- binding alanine: I38 (≠ V40), G40 (= G42), T41 (≠ G43), G42 (= G44), F226 (= F242), A227 (= A243), I229 (= I245)
- binding : E24 (≠ T26), G26 (= G28), F28 (≠ W30), D29 (≠ G31), M32 (≠ A34), A176 (≠ S185), R177 (≠ G186), A184 (≠ L193), A188 (≠ G197), L192 (= L201), Q294 (≠ L311), V297 (≠ L314)
6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
48% identity, 97% coverage: 15:473/474 of query aligns to 15:458/458 of 6f34A
- binding arginine: I40 (≠ V40), G42 (= G42), T43 (≠ G43), G44 (= G44), E115 (= E115), Y116 (= Y116), A119 (≠ S119), F228 (= F242), A229 (= A243), I231 (= I245), V314 (= V329)
- binding cholesterol: W201 (≠ Q208), Y202 (= Y209)
- binding : G28 (= G28), F30 (≠ W30), D31 (≠ G31), M34 (≠ A34), A178 (≠ S185), R179 (≠ G186), A186 (≠ L193), I187 (≠ L194), A190 (≠ G197), L194 (= L201), Q296 (≠ L311), V299 (≠ L314)
P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
32% identity, 88% coverage: 3:418/474 of query aligns to 11:438/629 of P30825
- N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9UHI5 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; hLAT2; Solute carrier family 7 member 8 from Homo sapiens (Human) (see 3 papers)
27% identity, 88% coverage: 9:423/474 of query aligns to 14:423/535 of Q9UHI5
- I53 (≠ V40) binding L-leucine
- Y93 (= Y79) mutation to A: Nearly complete reduction of glycine, L-alanine, and L-glutamine uptake. Minimal effect on the transport of L-isoleucine, L-histidine and L-tryptophan.
- N134 (≠ S119) Important for substrate specificity; binding L-tryptophan; mutation to Q: Reduces L-leucine uptake activity. Abolishes L-tryptophan uptake.; mutation to S: The substrate specificity changed dramatically reducing L-glutamine, glycine and L-alanine uptake activity thus mimicking the selectivity of SLC7A5.
- C154 (≠ L155) modified: Interchain (with C-210 in SLC3A2)
- W174 (= W175) mutation to A: Does not affect protein expression, plasma membrane localization, or L-alanine uptake.
- F243 (= F242) mutation to A: Abolishes leucine and tryptophan transport activities.
- G246 (≠ I245) Important for substrate specificity; binding L-leucine; mutation to S: Strong decrease in the uptake of large substrates L-tryptophan, L-glutamine, and L-histidine but increases the uptake of small neutral amino acids glycine and L-alanine.
- V302 (≠ P300) to I: found in a patient with age-related hearing loss; does not affect L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs142951280
- N395 (≠ T395) binding L-tryptophan; mutation to Q: Strongly reduces L-leucine uptake activity. Strongly reduces L-tryptophan uptake activity.
- Y396 (≠ L396) mutation to A: Strongly reduces L-leucine uptake activity.
- T402 (≠ V402) to M: found in a patient with age-related hearing loss; strongly decreased L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs758342760
- R418 (= R418) to C: found in a patient with age-related hearing loss; decreases L-alanine transport activity. Decreases L-tyrosine transport activity; dbSNP:rs146946494
Sites not aligning to the query:
- 460 V → E: found in a patient with age-related hearing loss; strongly decreases L-alanine transport activity. Decreases L-tyrosine transport activity. Decreases cell membrane localization
Q9QXW9 Large neutral amino acids transporter small subunit 2; L-type amino acid transporter 2; mLAT2; Solute carrier family 7 member 8 from Mus musculus (Mouse) (see paper)
25% identity, 88% coverage: 7:422/474 of query aligns to 12:421/531 of Q9QXW9
- Y130 (= Y116) mutation to A: Increases T2 import. Increases T3 and enables T4 import. Does not affect L-leucine and L-phenylalanine uptake.
- N133 (≠ S119) mutation to S: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake. Increases the export of both L-leucine and L-phenylalanine.
- F242 (= F242) mutation to W: Increases T2 import. Does not affect T3 import. Does not affect L-leucine and L-phenylalanine uptake.
Q7YQK4 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; L-type amino acid transporter 1; LAT1; Solute carrier family 7 member 5 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
26% identity, 89% coverage: 3:423/474 of query aligns to 24:428/503 of Q7YQK4
- C88 (= C71) mutation to S: No significant effect on inhibition by HgCl(2). Decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-183.
- C98 (≠ A78) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Slightly less decreased KM and Vmax for Phe; when associated with S-183.
- C160 (≠ A145) mutation to S: No change to KM or Vmax for Phe.
- C172 (≠ A166) mutation to S: No change to KM or Vmax for Phe.
- C174 (≠ A168) mutation to S: No change to KM or Vmax for Phe.
- C183 (= C177) mutation to S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe. Similar affect on KM and Vmax for Phe; when associated with S-88. Slightly less decreased KM and Vmax for Phe; when associated with S-98.
- G219 (vs. gap) mutation to D: Decreased KM and Vmax for Trp. Increased KM and Vmax for Phe; when associated with L-234.
- W234 (≠ R235) mutation to L: Decreased KM and Vmax for Trp. Increased KM but decreased Vmax for Phe. Increased KM and Vmax for Phe; when associated with D-219.
- C331 (≠ S328) mutation to S: No significant effect on inhibition by HgCl(2). Increased KM and Vmax for Phe.
- C377 (≠ I373) mutation to S: No significant effect on inhibition by HgCl(2).
- C403 (≠ A398) mutation to S: No significant effect on inhibition by HgCl(2).
Sites not aligning to the query:
- 439 C→S: Prevents insertion into the plasma membrane and possibly protein folding.
- 454 C→S: No significant effect on inhibition by HgCl(2). Slightly increased KM but slightly decreased Vmax for Phe.
- 492 C→S: No significant effect on inhibition by HgCl(2). Slightly decreased KM and Vmax for Phe.
7cmiB The lat2-4f2hc complex in complex with leucine (see paper)
26% identity, 82% coverage: 37:423/474 of query aligns to 10:383/458 of 7cmiB
7cmhB The lat2-4f2hc complex in complex with tryptophan (see paper)
26% identity, 82% coverage: 37:423/474 of query aligns to 10:383/458 of 7cmhB
7b00A Human lat2-4f2hc complex in the apo-state (see paper)
26% identity, 82% coverage: 37:423/474 of query aligns to 10:383/457 of 7b00A
Sites not aligning to the query:
Q01650 Large neutral amino acids transporter small subunit 1; 4F2 light chain; 4F2 LC; 4F2LC; CD98 light chain; Integral membrane protein E16; E16; L-type amino acid transporter 1; hLAT1; Solute carrier family 7 member 5; y+ system cationic amino acid transporter from Homo sapiens (Human) (see 3 papers)
26% identity, 87% coverage: 12:423/474 of query aligns to 35:432/507 of Q01650
- Y117 (= Y93) mutation to A: Strongly decreased leucine transport activity.
- C164 (≠ A145) modified: Interchain (with C-210 in SLC3A2)
- D223 (= D211) to V: in dbSNP:rs17853937
- N230 (≠ K227) to K: in dbSNP:rs1060250
- A246 (vs. gap) mutation to V: Nearly abolishes leucine transport activity.
- F252 (= F242) mutation to A: Nearly abolishes leucine transport activity.
- W257 (≠ F247) mutation to A: Nearly abolishes leucine transport activity.
- N258 (≠ E248) mutation to A: Decreased leucine transport activity.; mutation to D: Nearly abolishes leucine transport activity.
- Y259 (≠ A249) mutation to A: Strongly decreased leucine transport activity.
- E303 (≠ D298) mutation to K: Decreased leucine transport activity.
- P375 (≠ I366) mutation to L: Nearly abolishes leucine transport activity.
Sites not aligning to the query:
- 483:507 mutation Missing: Nearly abolishes leucine transport activity.
P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
23% identity, 85% coverage: 6:407/474 of query aligns to 1:382/461 of P76037