SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_036139934.1 NCBI__GCF_000768355.1:WP_036139934.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
37% identity, 93% coverage: 1:230/248 of query aligns to 4:230/285 of 4yerA

query
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4yerA

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binding adenosine-5'-diphosphate: F14 (= F11), F17 (≠ V18), N39 (= N40), G40 (= G41), G42 (= G43), K43 (= K44), T44 (= T45), T45 (= T46), T135 (≠ G136), F136 (= F137), S137 (= S138)

Q8R420 Phospholipid-transporting ATPase ABCA3; ATP-binding cassette sub-family A member 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Mus musculus (Mouse) (see paper)
34% identity, 90% coverage: 7:228/248 of query aligns to 535:755/1704 of Q8R420

query
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Q8R420

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Sites not aligning to the query:

292 E→V: Knockin new born mice are healthy and survive into adulthood without overt signs of respiratory distress. Knockin mice show a severe lung phenotype that begins with alveolar inflammatory cell infiltration at the early stage of the mouse life followed by aberrant lung remodeling with characteristics of diffuse parenchymal lung disease (DPLD)- and emphysema-like alveolar disruption in older mice.

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
35% identity, 90% coverage: 7:228/248 of query aligns to 535:755/1704 of Q99758

query
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Q99758

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N568 (= N40) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (= L51) to P: in SMDP3; uncertain significance
R605 (= R77) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (≠ N165) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

7w02A Cryo-em structure of atp-bound abca3 (see paper)
34% identity, 90% coverage: 7:228/248 of query aligns to 500:720/1566 of 7w02A

query
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7w02A

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binding adenosine-5'-triphosphate: F504 (= F11), N533 (= N40), G534 (= G41), G536 (= G43), K537 (= K44), T538 (= T45), T539 (= T46), Q578 (≠ D85), L630 (≠ F137), S631 (= S138)
binding magnesium ion: T538 (= T45), Q578 (≠ D85)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1277, 1305, 1306, 1307, 1308, 1309, 1310, 1344, 1395, 1398
binding magnesium ion: 1310, 1344

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
33% identity, 92% coverage: 1:229/248 of query aligns to 2049:2278/2435 of Q9BZC7

query
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Q9BZC7

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D

E

M

L

G

T

D

K

I

Y

L

A

D

D

R

K

Q

P

T

A

E

G

G

T

F

Y

S

S

Q

G

G

G

Q

N

R

K

T

R

K

K

T

L

A

S

I

T

A

A

R

I

A

A

L

L

V

I

H

G

D

Y

P

P

K

A

N

F

V

I

I

F

L

L

D

D

E

E

P

P

T

T

N

T

G

G

L

M

D

D

V

P

M

K

T

A

T

R

R

R

A

F

M

L

R

W

G

N

F

L

L

I

Q

L

Q

D

L

L

K

I

A

K

E

T

G

G

R

R

C

S

V

V

I

V

F

L

S

T

S

S

H

H

I

S

M

M

Q

E

E

E

V

C

A

E

A

A

L

L

C

C

D

T

R

R

I

L

V

A

I

I

I

M

A

V

K

N

G

G

R

R

V

L

V

R

A

C

A

L

G

G

T

S

A

I

D

Q

E

H

L

L

R

K

A

N

K

R

F

F

G

G

E

D

Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

Q8N139 ATP-binding cassette sub-family A member 6; EC 7.6.2.- from Homo sapiens (Human) (see 2 papers)
32% identity, 90% coverage: 6:228/248 of query aligns to 482:705/1617 of Q8N139

query
sites
Q8N139

D

N

L

V

H

K

K

K

A

E

F

Y

K

K

T

G

K

K

T

S

G

G

T

K

V

V

Q

E

A

A

V

L

E

K

G

G

V

L

S

L

F

F

E

D

A

I

R

Y

D

E

G

G

Q

Q

I

I

T

T

G

A

L

I

L

L

G

G

P

H

N

S

G

G

A

A

G

G

K

K

T

S

T

L

L

L

R

N

M

I

L

L

Y

N

T

G

L

L

M

S

T

V

P

P

D

T

R

E

G

G

N

S

V

V

T

T

V

I

-

Y

-

N

D

K

G

N

I

L

D

S

A

E

A

M

L

Q

D

D

P

L

A

E

V

I

R

R

R

K

A

I

L

T

G

G

V

V

L

C

P

P

D

Q

A

F

R

N

G

V

V

Q

Y

F

K

D

R

I

L

L

T

T

A

V

R

K

E

E

N

N

I

L

A

S

Y

L

F

F

G

A

E

K

L

I

H

K

G

G

L

I

S

H

A

L

T

K

D

E

I

V

A

E

R

Q

R

E

T

V

E

Q

V

R

L

I

A

L

K

L

A

E

L

L

D

D

M

M

G

Q

D

N

I

I

L

Q

D

D

R

N

Q

L

T

A

E

K

G

H

F

L

S

S

Q

E

G

G

Q

Q

R

K

T

R

K

K

T

L

A

T

I

F

A

G

R

I

A

T

L

I

V

L

H

G

D

D

P

P

K

Q

N

I

V

L

I

L

L

L

D

D

E

E

P

P

T

T

N

T

G

G

L

L

D

D

V

P

M

F

T

S

T

R

R

D

A

Q

M

V

R

W

G

S

F

L

L

L

Q

R

Q

E

L

R

K

R

A

A

E

D

G

-

R

H

C

V

V

I

I

L

F

F

S

S

S

T

H

Q

I

S

M

M

Q

D

E

E

V

A

A

D

A

I

L

L

C

A

D

D

R

R

I

K

V

V

I

I

I

M

A

S

K

N

G

G

R

R

V

L

V

K

A

C

A

A

G

G

T

S

A

S

D

M

E

F

L

L

R

K

A

R

K

R

F

W

G

G

Sites not aligning to the query:

282 V → I: in dbSNP:rs4968839
875 M → I: in dbSNP:rs7212506
940 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1322 N → S: in dbSNP:rs2302134

F1MWM0 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM protein; RmP; Retinal-specific ATP-binding cassette transporter; EC 7.6.2.1 from Bos taurus (Bovine) (see 2 papers)
35% identity, 90% coverage: 7:229/248 of query aligns to 1938:2161/2281 of F1MWM0

query
sites
F1MWM0

L

L

H

N

K

E

A

L

F

T

K

K

T

V

K

Y

T

S

G

G

T

T

V

S

Q

S

-

P

A

A

V

V

E

D

G

R

V

L

S

C

F

V

E

G

A

V

R

R

D

P

G

G

Q

E

I

C

T

F

G

G

L

L

G

G

P

V

N

N

G

G

A

A

G

G

K

K

T

T

L

F

R

K

M

M

L

L

Y

T

T

G

L

D

M

T

T

A

P

V

D

T

R

S

G

G

N

D

V

A

T

T

V

V

D

A

G

G

I

K

D

S

A

I

A

L

L

T

D

N

P

I

A

S

A

D

V

V

R

H

R

Q

A

S

L

M

G

G

V

Y

L

C

P

P

D

Q

A

F

R

D

G

A

V

I

Y

D

K

D

R

L

L

L

T

T

A

G

R

R

E

E

N

H

I

L

A

Y

Y

L

F

Y

G

A

E

R

L

L

H

R

G

G

L

V

S

P

A

A

T

E

D

E

I

I

A

E

R

R

R

V

T

T

E

N

V

W

L

S

A

I

K

Q

A

S

L

L

D

G

M

L

G

S

D

L

I

Y

L

A

D

D

R

R

Q

L

T

A

E

G

G

T

F

Y

S

S

Q

G

G

G

Q

N

R

K

T

R

K

K

T

L

A

S

I

T

A

A

R

I

A

A

L

L

V

I

H

G

D

C

P

P

K

P

N

L

V

V

I

L

L

L

D

D

E

E

P

P

T

T

N

T

G

G

L

M

D

D

V

P

M

Q

T

A

T

R

R

R

A

M

M

L

R

W

G

N

F

T

L

I

Q

M

Q

G

L

I

K

I

A

R

E

E

G

G

R

R

C

A

V

V

I

V

F

L

S

T

S

S

H

H

I

S

M

M

Q

E

E

E

V

C

A

E

A

A

L

L

C

C

D

T

R

R

I

L

V

A

I

I

I

M

A

V

K

K

G

G

R

A

V

F

V

Q

A

C

A

L

G

G

T

T

A

I

D

Q

E

H

L

L

R

K

A

S

K

K

F

F

G

G

E

D

Sites not aligning to the query:

415 modified: carbohydrate, N-linked (Hex...) asparagine
504 modified: carbohydrate, N-linked (Hex...) asparagine
901 modified: Phosphothreonine; T→A: Decreases expression level. Affects subcellular location.
1185 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1309 Cleavage; by trypsin
1313 modified: Phosphothreonine; T→A: Does not affect subcellular location. Does not affect expression level. Does not affect ATPase activity. Reduces the stimulating effect of all-trans-retinal on ATP hydrolysis.
1317 modified: Phosphoserine; S→A: Does not affect subcellular location. Does not affect expression level. Affects both the basal and stimulated ATPase activity.
1319 modified: Phosphoserine
1455 modified: carbohydrate, N-linked (Hex...) asparagine
1527 modified: carbohydrate, N-linked (Hex...) asparagine
1660 modified: carbohydrate, N-linked (Hex...) asparagine

8f5bA Human abca4 structure in complex with amp-pnp
37% identity, 84% coverage: 20:228/248 of query aligns to 737:944/1924 of 8f5bA

query
sites
8f5bA

A

A

V

V

E

D

G

R

V

L

S

N

F

I

E

T

A

F

R

Y

D

E

G

N

Q

Q

I

I

T

T

G

A

L

F

L

L

G

G

P

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

T
|
T

T

T

L

L

R

S

M

I

L

L

Y

T

T

G

L

L

M

L

T

P

P

P

D

T

R

S

G

G

N

T

V

V

T

L

V

V

D

G

G

G

I

R

D

D

A

I

A

E

L

T

D

S

P

L

A

D

A

A

V

V

R

R

R

Q

A

S

L

L

G

G

V

M

L

C

P

P

D
x
Q

A

H

R

N

G

I

V

L

Y

F

K

H

R

H

L

L

T

T

A

V

R

A

E

E

N

H

I

M

A

L

Y

F

F

Y

G

A

E

Q

L

L

H

K

G

G

L

K

S

S

A

Q

T

E

D

E

I

A

A

Q

R

L

R

E

T

M

E

E

V

A

L

M

A

L

K

E

A

D

L

T

D

G

M

L

G

H

D

H

I

K

L

R

D

N

R

E

Q

E

T

A

E

Q

G
x
D

F

L

S

S

Q

G

G

G

Q

M

R

Q

T

R

K

K

T

L

A

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

D

D

P

A

K

K

N

V

V

V

I

I

L

L

D

D

E

E

P

P

T

T

N

S

G

G

L

V

D

D

V

P

M

Y

T

S

T

R

R

R

A

S

M

I

R

W

G

D

F

L

L

L

Q

L

Q

K

L

Y

K

R

A

S

E

-

G

G

R

R

C

T

V

I

I

I

F

M

S

S

S

T

H

H

I

H

M

M

Q

D

E

E

V

A

A

D

A

L

L

L

C

G

D

D

R

R

I

I

V

A

I

I

A

A

K

Q

G

G

R

R

V

L

V

Y

A

C

A

S

G

G

T

T

A

P

D

L

E

F

L

L

R

K

A

N

K

C

F

F

G

G

binding phosphoaminophosphonic acid-adenylate ester: N757 (= N40), G758 (= G41), G760 (= G43), K761 (= K44), T762 (= T45), T763 (= T46), Q802 (≠ D85), D853 (≠ G136)
binding magnesium ion: T762 (= T45), Q802 (≠ D85)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 1652, 1653, 1654, 1655, 1656, 1657, 1697, 1748, 1750
binding magnesium ion: 1657, 1697

7e7oA Cryo-em structure of human abca4 in nrpe-bound state (see paper)
37% identity, 84% coverage: 20:228/248 of query aligns to 824:1031/2003 of 7e7oA

query
sites
7e7oA

A

A

V

V

E

D

G

R

V

L

S

N

F

I

E

T

A

F

R

Y

D

E

G

N

Q

Q

I

I

T

T

G

A

L

F

L

L

G

G

P

H

N

N

G

G

A

A

G

G

K

K

T

T

L

L

R

S

M

I

L

L

Y

T

T

G

L

L

M

L

T

P

P

P

D

T

R

S

G

G

N

T

V

V

T

L

V

V

D

G

G

G

I

R

D

D

A

I

A

E

L

T

D

S

P

L

A

D

A

A

V

V

R

R

R

Q

A

S

L

L

G

G

V

M

L

C

P

P

D

Q

A

H

R

N

G

I

V

L

Y

F

K

H

R

H

L

L

T

T

A

V

R

A

E

E

N

H

I

M

A

L

Y

F

F

Y

G

A

E

Q

L

L

H

K

G

G

L

K

S

S

A

Q

T

E

D

E

I

A

A

Q

R

L

R

E

T

M

E

E

V

A

L

M

A

L

K

E

A

D

L

T

D

G

M

L

G

H

D

H

I

K

L

R

D

N

R

E

Q

E

T

A

E

Q

G

D

F

L

S

S

Q

G

G

G

Q

M

R

Q

T

R

K

K

T

L

A

S

I

V

A

A

R

I

A

A

L

F

V

V

H

G

D

D

P

A

K

K

N

V

V

V

I

I

L

L

D

D

E

E

P

P

T

T

N

S

G

G

L

V

D

D

V

P

M

Y

T

S

T

R

R

R

A

S

M

I

R

W

G

D

F

L

L

L

Q

L

Q

K

L

Y

K

R

A

S

E

-

G

G

R

R

C

T

V

I

I

I

F

M

S

S

S

T

H

H

I

H

M

M

Q

D

E

E

V

A

A

D

A

L

L

L

C

G

D

D

R

R

I

I

V

A

I

I

A

A

K

Q

G

G

R

R

V

L

V

Y

A

C

A

S

G

G

T

T

A

P

D

L

E

F

L

L

R

K

A

N

K

C

F

F

G

G

Sites not aligning to the query:

binding [(2S)-3-[2-[(E)-[(2E,4E,6E,8E)-3,7-dimethyl-9-(2,6,6-trimethylcyclohexen-1-yl)nona-2,4,6,8-tetraenylidene]amino]ethoxy-oxidanyl-phosphoryl]oxy-2-[(Z)-octadec-9-enoyl]oxy-propyl] (Z)-octadec-9-enoate: 285, 290, 526, 527, 589, 592, 1454

P78363 Retinal-specific phospholipid-transporting ATPase ABCA4; ATP-binding cassette sub-family A member 4; RIM ABC transporter; RIM proteinv; RmP; Retinal-specific ATP-binding cassette transporter; Stargardt disease protein; EC 7.6.2.1 from Homo sapiens (Human) (see 43 papers)
37% identity, 84% coverage: 20:228/248 of query aligns to 945:1152/2273 of P78363

query
sites
P78363

A
|
A

V
|
V

E
x
D

G
x
R

V
x
L

S
x
N

F
x
I

E
x
T

A
x
F

R
x
Y

D
x
E

G
x
N

Q
|
Q

I
|
I

T
|
T

G
x
A

L
x
F

L
|
L

G
|
G

P
x
H

N
|
N

G
|
G

A
|
A

G
|
G

K
|
K

T
|
T

L
|
L

R
x
S

M
x
I

L
|
L

Y
x
T

T
x
G

L
|
L

M
x
L

T
x
P

P
|
P

D
x
T

R
x
S

G
|
G

N
x
T

V
|
V

T
x
L

V
|
V

D
x
G

G
|
G

I
x
R

D
|
D

A
x
I

A
x
E

L
x
T

D
x
S

P
x
L

A
x
D

A
|
A

V
|
V

R
|
R

R
x
Q

A
x
S

L
|
L

G
|
G

V
x
M

L
x
C

P
|
P

D
x
Q

A
x
H

R
x
N

G
x
I

V
x
L

Y
x
F

K
x
H

R
x
H

L
|
L

T
|
T

A
x
V

R
x
A

E
|
E

N
x
H

I
x
M

A
x
L

Y
x
F

F
x
Y

G
x
A

E
x
Q

L
|
L

H
x
K

G
|
G

L
x
K

S
|
S

A
x
Q

T
x
E

D
x
E

I
x
A

A
x
Q

R
x
L

R
x
E

T
x
M

E
|
E

V
x
A

L
x
M

A
x
L

K
x
E

A
x
D

L
x
T