SitesBLAST
Comparing WP_036376281.1 NCBI__GCF_000015305.1:WP_036376281.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4p4gA Crystal structure of mycobacterium tuberculosis shikimate dehydrogenase
69% identity, 97% coverage: 5:267/270 of query aligns to 1:261/265 of 4p4gA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S18), S16 (= S20), T61 (= T65), K65 (= K69), N86 (= N90), D101 (= D105), Y211 (= Y217), L236 (= L242), Q239 (= Q245)
7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
32% identity, 97% coverage: 6:267/270 of query aligns to 403:679/682 of 7tbvA
Sites not aligning to the query:
6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
32% identity, 97% coverage: 5:267/270 of query aligns to 1264:1548/1555 of 6hqvA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S1277 (= S18), S1279 (= S20), T1323 (= T65), K1327 (= K69), N1348 (= N90), D1368 (= D105), Q1526 (= Q245)
Sites not aligning to the query:
- active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
- binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
- binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
- binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 874, 923, 924, 979
- binding zinc ion: 187, 264, 280
3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
25% identity, 96% coverage: 7:264/270 of query aligns to 2:249/258 of 3dooA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S18), S15 (= S20), N58 (≠ S63), T60 (= T65), K64 (= K69), N85 (= N90), D100 (= D105), F227 (≠ L242), Q230 (= Q245)
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
26% identity, 96% coverage: 7:264/270 of query aligns to 2:258/269 of Q5HNV1
- SLS 13:15 (≠ SRS 18:20) binding shikimate
- T60 (= T65) binding shikimate
- N85 (= N90) binding shikimate
- D100 (= D105) binding shikimate
- Y211 (= Y217) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q245) binding shikimate
P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
29% identity, 97% coverage: 5:266/270 of query aligns to 1289:1578/1583 of P07547
Sites not aligning to the query:
- 44:46 binding NAD(+)
- 81:84 binding NAD(+)
- 114:116 binding NAD(+)
- 119 binding NAD(+)
- 139:140 binding NAD(+)
- 161 binding NAD(+)
- 179:182 binding NAD(+)
- 190 binding NAD(+)
- 194 binding Zn(2+)
- 271 binding Zn(2+)
- 287 binding Zn(2+)
2o7sA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
31% identity, 93% coverage: 10:261/270 of query aligns to 239:482/500 of 2o7sA
- binding 3-dehydroshikimate: I239 (≠ V10), S247 (= S18), S249 (= S20), T292 (= T65), K296 (= K69), N317 (= N90), D334 (= D105), Y438 (= Y217), Q466 (= Q245), Q470 (= Q249)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I293 (≠ M66), P294 (= P67), K296 (= K69), D334 (= D105), G354 (= G128), G355 (= G129), A356 (≠ T130), N374 (≠ A150), R375 (= R151), T376 (≠ H152), R379 (≠ K155), T409 (≠ V184), S410 (≠ A185), M411 (vs. gap), A436 (= A215), M462 (= M241), F463 (≠ L242)
Sites not aligning to the query:
3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
28% identity, 96% coverage: 10:267/270 of query aligns to 18:290/291 of 3tozA
- binding nicotinamide-adenine-dinucleotide: G137 (= G126), A138 (≠ S127), G139 (= G128), G140 (= G129), A141 (≠ T130), N161 (≠ A150), R162 (= R151), D164 (≠ A153), F166 (vs. gap), T210 (vs. gap), G211 (vs. gap), V212 (vs. gap), M214 (vs. gap), F217 (≠ V194), V238 (≠ A215), Y240 (= Y217), G261 (= G238), M264 (= M241), M265 (≠ L242)
Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
28% identity, 96% coverage: 10:267/270 of query aligns to 18:290/291 of Q8Y9N5
- SLS 26:28 (≠ SRS 18:20) binding shikimate
- NRKD 161:164 (≠ ARHA 150:153) binding NAD(+)
- M214 (vs. gap) binding NADP(+)
3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
28% identity, 96% coverage: 10:267/270 of query aligns to 15:287/288 of 3tnlA
- binding nicotinamide-adenine-dinucleotide: M71 (= M66), G134 (= G126), A135 (≠ S127), G136 (= G128), G137 (= G129), A138 (≠ T130), N158 (≠ A150), R159 (= R151), D161 (≠ A153), F163 (vs. gap), T207 (vs. gap), V209 (vs. gap), M211 (vs. gap), F214 (≠ V194), V235 (≠ A215), Y237 (= Y217), M261 (= M241), M262 (≠ L242)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S18), S25 (= S20), N68 (≠ S63), S70 (≠ T65), K74 (= K69), N95 (= N90), D110 (= D105), Q265 (= Q245)
2gptA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate (see paper)
31% identity, 93% coverage: 10:261/270 of query aligns to 239:480/498 of 2gptA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: I239 (≠ V10), S247 (= S18), S249 (= S20), T292 (= T65), K296 (= K69), N317 (= N90), D334 (= D105), Y436 (= Y217), Q464 (= Q245), Q468 (= Q249)
Sites not aligning to the query:
2o7qA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
29% identity, 93% coverage: 10:261/270 of query aligns to 239:483/501 of 2o7qA
Sites not aligning to the query:
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
28% identity, 99% coverage: 1:267/270 of query aligns to 7:281/287 of 1nvtB
- active site: K75 (= K69), D111 (= D105)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ M66), G135 (≠ S127), G137 (= G129), G138 (≠ T130), A139 (= A131), N157 (≠ A150), R158 (= R151), T159 (≠ H152), K162 (= K155), A200 (≠ T193), T201 (≠ V194), P202 (= P195), I203 (≠ A196), M205 (≠ A198), L229 (≠ A215), Y231 (= Y217), M255 (= M241), L256 (= L242)
- binding zinc ion: E22 (≠ A16), H23 (= H17)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
28% identity, 99% coverage: 1:267/270 of query aligns to 7:281/287 of 1nvtA
- active site: K75 (= K69), D111 (= D105)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (≠ S127), A139 (= A131), N157 (≠ A150), R158 (= R151), T159 (≠ H152), K162 (= K155), A200 (≠ T193), T201 (≠ V194), P202 (= P195), I203 (≠ A196), M205 (≠ A198), L229 (≠ A215), Y231 (= Y217), G252 (= G238), M255 (= M241), L256 (= L242)
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
28% identity, 99% coverage: 1:267/270 of query aligns to 2:276/282 of Q58484
1o9bA Quinate/shikimate dehydrogenase ydib complexed with nadh (see paper)
28% identity, 96% coverage: 10:267/270 of query aligns to 6:278/280 of 1o9bA
- binding 1,4-dihydronicotinamide adenine dinucleotide: A126 (≠ S127), G127 (= G128), G128 (= G129), A129 (≠ T130), R150 (= R151), F154 (≠ K155), K199 (≠ P195), V200 (≠ A196), M202 (≠ A198),