SitesBLAST
Comparing WP_036832369.1 NCBI__GCF_000775615.1:WP_036832369.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 74% coverage: 65:297/316 of query aligns to 61:295/308 of 5mhaB
- binding 2-Ketohexanoic acid: R66 (≠ S70), R226 (= R228), H274 (= H276), Y282 (= Y284)
- binding (2R)-2-hydroxyhexanoic acid: R66 (≠ S70), A67 (= A71), G68 (= G72), H91 (= H95), Y282 (= Y284)
- binding magnesium ion: F212 (= F214), E213 (= E215), M215 (= M217), D243 (≠ Q245)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ K92), L143 (≠ A145), G144 (= G146), T145 (≠ A147), L146 (≠ I148), R165 (≠ S167), R166 (≠ Y168), S167 (= S169), P180 (≠ Q182), T197 (≠ L199), P198 (= P200), T203 (= T205), V224 (≠ M226), A225 (≠ G227), R226 (= R228), H274 (= H276), S276 (= S278)
5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
30% identity, 74% coverage: 65:297/316 of query aligns to 61:295/308 of 5mhaA
- binding 2-Ketohexanoic acid: R66 (≠ S70), A67 (= A71), G68 (= G72), H91 (= H95), R226 (= R228), H274 (= H276), Y282 (= Y284)
- binding magnesium ion: T132 (≠ E134), A134 (≠ T136)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ K92), L143 (≠ A145), G144 (= G146), T145 (≠ A147), L146 (≠ I148), R165 (≠ S167), R166 (≠ Y168), S167 (= S169), P180 (≠ Q182), T197 (≠ L199), P198 (= P200), T203 (= T205), V224 (≠ M226), A225 (≠ G227), R226 (= R228), H274 (= H276), S276 (= S278)
5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
30% identity, 74% coverage: 65:297/316 of query aligns to 61:295/308 of 5mh5A
- binding 2-Ketohexanoic acid: R66 (≠ S70), A67 (= A71), G68 (= G72), H91 (= H95), R226 (= R228), H274 (= H276), Y282 (= Y284)
- binding magnesium ion: T132 (≠ E134), A134 (≠ T136), F212 (= F214), E213 (= E215), M215 (= M217), D243 (≠ Q245)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A67 (= A71), G68 (= G72), T88 (≠ K92), G142 (= G144), L143 (≠ A145), G144 (= G146), T145 (≠ A147), L146 (≠ I148), R165 (≠ S167), R166 (≠ Y168), S167 (= S169), T197 (≠ L199), P198 (= P200), T203 (= T205), V224 (≠ M226), A225 (≠ G227), R226 (= R228), H274 (= H276), S276 (= S278)
5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
30% identity, 74% coverage: 65:297/316 of query aligns to 59:293/306 of 5mh6A
- binding 2-Ketohexanoic acid: R64 (≠ S70), A65 (= A71), G66 (= G72), H89 (= H95), R224 (= R228), H272 (= H276), Y280 (= Y284)
- binding magnesium ion: T130 (≠ E134), A132 (≠ T136), F210 (= F214), E211 (= E215), M213 (= M217), G225 (= G229), P226 (≠ D230), V228 (= V232), E230 (≠ N234), D241 (≠ Q245), S251 (≠ E255)
- binding nicotinamide-adenine-dinucleotide: A65 (= A71), G66 (= G72), T86 (≠ K92), H89 (= H95), G142 (= G146), T143 (≠ A147), L144 (≠ I148), R164 (≠ Y168), P196 (= P200), T201 (= T205), V222 (≠ M226), A223 (≠ G227), R224 (= R228), H272 (= H276), S274 (= S278)
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
32% identity, 74% coverage: 44:277/316 of query aligns to 53:290/324 of 2gcgA
- active site: L103 (≠ H95), R241 (= R228), D265 (= D252), E270 (= E257), H289 (= H276)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (= L46), S78 (= S70), V79 (≠ A71), G80 (= G72), R241 (= R228), H289 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (≠ A71), T107 (≠ M99), G156 (= G144), G158 (= G146), I160 (= I148), G180 (= G170), R181 (≠ K171), R184 (≠ H174), C212 (≠ L199), S213 (≠ P200), T218 (= T205), I239 (≠ M226), R241 (= R228), D265 (= D252), H289 (= H276)
Sites not aligning to the query:
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
32% identity, 74% coverage: 44:277/316 of query aligns to 57:294/328 of Q9UBQ7
- VG 83:84 (≠ AG 71:72) binding substrate
- GRI 162:164 (≠ GAI 146:148) binding NADP(+)
- RQPR 185:188 (≠ KEKH 171:174) binding NADP(+)
- S217 (≠ P200) binding NADP(+)
- I243 (≠ M226) binding NADP(+)
- R245 (= R228) binding substrate
- D269 (= D252) binding substrate
Sites not aligning to the query:
- 293:296 binding substrate
- 295 binding NADP(+)
4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
29% identity, 84% coverage: 51:316/316 of query aligns to 55:316/316 of 4zqbB
- active site: L99 (≠ H95), R231 (= R228), E260 (= E257), H279 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (≠ T89), M103 (= M99), G147 (= G144), L148 (≠ A145), G149 (= G146), E150 (≠ A147), L151 (≠ I148), W169 (≠ I166), S170 (= S167), R171 (≠ Y168), S172 (= S169), K174 (= K171), L202 (= L199), P203 (= P200), F229 (≠ M226), R231 (= R228), H279 (= H276), S281 (= S278), A282 (≠ G279), Y316 (= Y316)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
28% identity, 79% coverage: 50:300/316 of query aligns to 55:312/334 of 5aovA
- active site: L100 (≠ H95), R241 (= R228), D265 (= D252), E270 (= E257), H288 (= H276)
- binding glyoxylic acid: Y74 (≠ L69), A75 (≠ S70), V76 (≠ A71), G77 (= G72), R241 (= R228), H288 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A71), T104 (≠ M99), F158 (≠ A145), G159 (= G146), R160 (≠ A147), I161 (= I148), S180 (= S167), R181 (≠ Y168), A211 (≠ V198), V212 (≠ L199), P213 (= P200), T218 (= T205), I239 (≠ M226), A240 (≠ G227), R241 (= R228), H288 (= H276), G290 (≠ S278)
Sites not aligning to the query:
4z0pA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
33% identity, 58% coverage: 99:281/316 of query aligns to 99:284/316 of 4z0pA
- active site: R231 (= R228), G250 (= G247), D255 (= D252), E260 (= E257), H279 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: M99 (= M99), M144 (≠ A145), G145 (= G146), V146 (≠ A147), L147 (≠ I148), W165 (≠ I166), S166 (= S167), R167 (≠ Y168), S168 (= S169), R170 (≠ H174), L197 (= L199), P198 (= P200), A229 (≠ M226), G230 (= G227), R231 (= R228), H279 (= H276), A281 (≠ S278), A282 (≠ G279)
- binding oxalic acid: H114 (≠ E114), R115 (≠ K115), R231 (= R228), H279 (= H276)
Sites not aligning to the query:
4weqA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADP and sulfate (see paper)
33% identity, 58% coverage: 99:281/316 of query aligns to 99:284/316 of 4weqA
- active site: R231 (= R228), G250 (= G247), D255 (= D252), E260 (= E257), H279 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (= M99), M144 (≠ A145), G145 (= G146), V146 (≠ A147), L147 (≠ I148), W165 (≠ I166), S166 (= S167), R167 (≠ Y168), S168 (= S169), R170 (≠ H174), L197 (= L199), P198 (= P200), A229 (≠ M226), G230 (= G227), R231 (= R228), D255 (= D252), H279 (= H276), A281 (≠ S278)
Sites not aligning to the query:
5tsdA Crystal structure of NADPH-dependent 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH and oxalate
29% identity, 67% coverage: 71:283/316 of query aligns to 80:286/316 of 5tsdA
- active site: E260 (= E257), H279 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (≠ A80), M99 (= M99), G143 (= G144), L144 (≠ A145), G145 (= G146), I146 (≠ A147), L147 (≠ I148), W165 (≠ I166), S166 (= S167), R167 (≠ Y168), T168 (≠ S169), K170 (= K171), L197 (= L199), P198 (= P200), A229 (≠ M226), G230 (= G227), R231 (= R228), D255 (= D252), H279 (= H276)
- binding oxalic acid: R231 (= R228), H279 (= H276)
Sites not aligning to the query:
5bqfA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADP, hepes and l(+)-tartaric acid
29% identity, 67% coverage: 71:283/316 of query aligns to 81:287/317 of 5bqfA
- active site: E261 (= E257), H280 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R90 (≠ A80), M100 (= M99), G144 (= G144), L145 (≠ A145), G146 (= G146), I147 (≠ A147), L148 (≠ I148), W166 (≠ I166), S167 (= S167), R168 (≠ Y168), T169 (≠ S169), L198 (= L199), P199 (= P200), A230 (≠ M226), G231 (= G227), R232 (= R228), H280 (= H276), A283 (≠ G279)
Sites not aligning to the query:
4xcvA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH
29% identity, 67% coverage: 71:283/316 of query aligns to 81:287/317 of 4xcvA
- active site: E261 (= E257), H280 (= H276)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R90 (≠ A80), M100 (= M99), G144 (= G144), L145 (≠ A145), G146 (= G146), I147 (≠ A147), L148 (≠ I148), W166 (≠ I166), S167 (= S167), R168 (≠ Y168), T169 (≠ S169), K171 (= K171), L198 (= L199), P199 (= P200), A230 (≠ M226), G231 (= G227), R232 (= R228), H280 (= H276), A283 (≠ G279)
Sites not aligning to the query:
7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
28% identity, 69% coverage: 99:316/316 of query aligns to 100:313/313 of 7jqhA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: M100 (= M99), A145 (= A145), G146 (= G146), V147 (≠ A147), L148 (≠ I148), W166 (≠ I166), S167 (= S167), R168 (≠ Y168), T169 (≠ S169), K171 (= K171), L199 (= L199), P200 (= P200), L226 (≠ M226), A227 (≠ G227), R228 (= R228), D252 (= D252), H276 (= H276), A279 (≠ G279), Y313 (= Y316)
Sites not aligning to the query:
7jqiA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with alpha-ketoglutarate and NADP+
28% identity, 69% coverage: 99:316/316 of query aligns to 99:312/312 of 7jqiA
- binding 2-oxoglutaric acid: R227 (= R228), H275 (= H276)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (= M99), A144 (= A145), G145 (= G146), V146 (≠ A147), L147 (≠ I148), W165 (≠ I166), S166 (= S167), R167 (≠ Y168), T168 (≠ S169), K170 (= K171), L198 (= L199), P199 (= P200), L225 (≠ M226), R227 (= R228), H275 (= H276), A278 (≠ G279), Y312 (= Y316)
Sites not aligning to the query:
6p35A 2.5 angstrom structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with 2-keto arginine and NADP
28% identity, 69% coverage: 99:316/316 of query aligns to 99:312/312 of 6p35A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: M99 (= M99), A144 (= A145), G145 (= G146), V146 (≠ A147), L147 (≠ I148), W165 (≠ I166), S166 (= S167), R167 (≠ Y168), T168 (≠ S169), K170 (= K171), L198 (= L199), P199 (= P200), L225 (≠ M226), R227 (= R228), H275 (= H276), A277 (≠ S278), A278 (≠ G279), Y312 (= Y316)
- binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: R227 (= R228), H275 (= H276), T280 (≠ S281)
Sites not aligning to the query:
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
30% identity, 73% coverage: 57:288/316 of query aligns to 58:290/304 of 1wwkA