SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_036833137.1 NCBI__GCF_000775615.1:WP_036833137.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

1sazA Membership in the askha superfamily: enzymological properties and crystal structure of butyrate kinase 2 from thermotoga maritima (see paper)
45% identity, 97% coverage: 4:357/365 of query aligns to 3:357/375 of 1sazA

query
sites
1sazA

R

R

V

I

L

L

A

T

I

I

N

N

P

P

G

G

A

S

T

T

S

S

T

T

K

K

V

L

A

S

C

I

Y

F

E

E

N

D

D

E

E

R

L

M

T

V

F

K

K

M

K

Q

E

N

F

F

T

S

Y

H

H

S

Y

P

E

D

T

E

I

L

S

G

Q

R

Y

F

D

Q

S

K

I

I

M

L

Q

D

Q

Q

Y

L

H

E

L

F

R

R

Y

E

Q

K

D

I

I

A

A

R

T

Q

F

F

L

V

Q

E

E

E

M

T

D

G

M

Y

K

S

I

L

G

S

S

S

F

F

D

S

A

A

V

F

V

V

G

S

R

R

G

G

L

L

P

D

P

P

V

I

K

P

A

G

G

G

A

V

Y

Y

R

L

V

V

N

D

E

G

A

L

M

M

L

I

D

K

C

T

L

L

E

K

H

S

R

G

P

K

V

N

L

G

Q

E

H

H

A

A

S

S

N

N

L

L

G

G

A

A

S

I

L

-

A

-

K

-

G

-

I

I

A

A

E

H

V

R

F

F

G

S

T

S

E

E

D

T

-

G

C

V

Q

P

A

A

F

Y

I

V

Y

V

D

D

P

P

V

V

T

V

V

V

D

D

Q

E

M

M

E

E

D

D

V

V

A

A

R

R

I

V

S

S

G

G

S

H

A

P

S

N

I

Y

E

Q

R

R

K

K

S

S

I

I

G

F

H
|
H

V

A

L

L

N

N

M

Q

R

K

A

T

V

V

S

A

M

K

K

E

I

V

A

A

D

-

E

R

Q

M

L

M

G

N

K

K

P

R

Y

Y

E

E

S

M

R

N

L

L

V

V

A

A

H

H

I

M

G
|
G

S

I

S

S

A

I

S

A

A

A

H

H

R

R

N

K

G

G

R

R

I

V

I

I

D

D

L

V

V

N

S

N

-

A

-

L

D

D

D

G

E

D

G

G

L

P

F

F

S

T

T

P

E

E

R
|
R

S

S

G

G

G

T

L

L

P

P

L

L

K

T

E

Q

I

L

I

V

P

D

M

L

C

C

F

F

-

S

-

G

Q

K

R

F

T

T

Q

Y

K

E

E

E

M

M

T

K

E

K

L

R

T

I

R

V

K

G

K

N

G

G

L

L

V

V

S

A

Y

Y

F

L

G

G

T

T

N
x
S

D
|
D

A
|
A

R
|
R

I

E

V

V

E

V

E

R

K

R

A

I

K

K

N

Q

G

G

D

D

E

E

Q

W

A

A

Q

K

L

R

I

V

L

Y

R

R

A

A

M

M

A

A

Y

Y

Q

Q

I

I

A

A

K

K

T

W

I

I

G

G

E

K

L

M

A

A

T

A

V

V

L

L

C

K

G

G

K

E

V

V

D

D

A

F

I

I

I

V

L

L

T

T

G

G

G
|
G

L
|
L

A

A

Y
x
H

-

E

S

K

E

E

Y

F

V

L

M

V

N

P

M

W

V

I

K

T

E

K

R

R

V

V

S

S

F

F

L

I

A

A

P

P

V

V

H

L

I

V

V

F

P

P

G

G

E

S

E

N

E

E

L

E

L

K

A

A

L

L

A

A

K

L

G

S

A

A

R

L

R

R

V

V

L

L

T

R

G

G

Q

E

E

E

H

K

A

P

H

K

T

N

F

Y

I

S

E

E

active site: R214 (= R217)
binding phosphomethylphosphonic acid adenylate ester: H154 (= H158), G184 (= G189), G185 (= G190), G186 (= G191), S254 (≠ N255), D255 (= D256), A256 (= A257), R257 (= R258), G304 (= G305), L305 (= L306), H307 (≠ Y308)

1tuuA Acetate kinase crystallized with atpgs (see paper)
35% identity, 37% coverage: 174:307/365 of query aligns to 195:333/399 of 1tuuA

query
sites
1tuuA

L

L

G

G

K

K

P

P

Y

A

E

E

S

T

R

K

L

I

V

I

V

T

A

C

H

H

I

L

G
|
G

G

N

G
|
G

S

S

A

I

S

T

A

A

H

V

R

E

N

G

G

G

R

K

I

S

I

V

D

E

L

T

-

S

-

M

-

G

V

F

S

T

D

P

D

L

E

E

G

G

L

L

F

A

S

M

T

G

E

T

R
|
R

S

C

G

G

G

S

L

I

P

D

L

-

K

P

E

A

I

I

I

V

P

P

M

F

C

L

F

M

Q

E

R

K

-

E

-

G

-

L

T

T

Q

T

K

R

E

E

M

I

T

D

E

T

L

L

T

M

R

N

K

K

G

S

G

G

L

V

V

L

S

G

Y

V

F

S

G

G

-

L

T

S

N

N

D
|
D

A
x
F

R
|
R

I

D

V

L

E

D

E

E

K

A

A

A

K

S

N

K

G

G

D

N

E

R

Q

K

A

A

Q

E

L

L

I

A

L

L

R

E

A

I

M

F

A

A

Y

Y

Q

K

I

V

A

K

K

K

T

F

I

I

G

G

E

E

L

Y

A

S

T

A

V

V

L

L

C

N

G

G

K

-

V

A

D

D

A

A

I

V

L

F

T

T

G

A

G
|
G

L
x
I

A

G

active site: R241 (= R217)
binding adenosine-5'-diphosphate: G210 (= G189), D283 (= D256), F284 (≠ A257), R285 (= R258), G331 (= G305), I332 (≠ L306)
binding sulfate ion: G212 (= G191)

Sites not aligning to the query:

active site: 7, 91, 180, 384
binding adenosine-5'-diphosphate: 14, 335
binding sulfate ion: 91, 180

P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
35% identity, 37% coverage: 174:307/365 of query aligns to 195:333/408 of P38502

query
sites
P38502

L

L

G

G

K

K

P

P

Y

A

E

E

S

T

R

K

L

I

V

I

V

T

A

C

H

H

I

L

G

G

G
x
N

G

G

S

S

A

I

S

T

A

A

H

V

R

E

N

G

G

G

R

K

I

S

I

V

D

E

L

T

-

S

-

M

-

G

V

F

S

T

D
x
P

D

L

E

E

G

G

L

L

F

A

S

M

T

G

E

T

R
|
R

S

C

G

G

G

S

L

I

P

D

L

-

K

P

E

A

I

I

I

V

P

P

M

F

C

L

F

M

Q

E

R

K

-

E

-

G

-

L

T

T

Q

T

K

R

E

E

M

I

T

D

E

T

L

L

T

M

R

N

K

K

G

S

G

G

L

V

V

L

S

G

Y

V

F

S

G

G

-

L

T

S

N

N

D

D

A

F

R

R

I

D

V

L

E

D

E

E

K

A

A

A

K

S

N

K

G

G

D

N

E

R

Q

K

A

A

Q

E

L

L

I

A

L

L

R

E

A

I

M

F

A

A

Y

Y

Q

K

I

V

A

K

K

K

T

F

I

I

G

G

E

E

L

Y

A

S

T

A

V

V

L

L

C

N

G

G

K

-

V

A

D

D

A

A

I

V

L

F

T

T

G

A

G

G

L

I

A

G

N211 (≠ G190) mutation to A: Slightly reduced enzyme activity.
P232 (≠ D208) mutation to A: Decreases affinity for acetate.
R241 (= R217) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.

Sites not aligning to the query:

7 N→A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; N→D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
10 mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
12 S→A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; S→T: Decreases catalytic activity. Strongly decreases affinity for acetate.
14 K→A: Strongly decreases enzyme activity.; K→R: Reduces enzyme activity.
91 mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
93 V→A: Decreases affinity for acetate.
122 L→A: Decreases affinity for acetate.
148 active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
179 F→A: Decreases affinity for acetate.
384 E→A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.

1tuuB Acetate kinase crystallized with atpgs (see paper)
35% identity, 37% coverage: 174:307/365 of query aligns to 195:333/398 of 1tuuB

query
sites
1tuuB

L

L

G

G

K

K

P

P

Y

A

E

E

S

T

R

K

L

I

V

I

V

T

A

C

H

H

I

L

G

G

G

N

G
|
G

S

S

A

I

S

T

A

A

H

V

R

E

N

G

G

G

R

K

I

S

I

V

D

E

L

T

-

S

-

M

-

G

V

F

S

T

D

P

D

L

E

E

G

G

L

L

F

A

S

M

T

G

E

T

R
|
R

S

C

G

G

G

S

L

I

P

D

L

-

K

P

E

A

I

I

I

V

P

P

M

F

C

L

F

M

Q

E

R

K

-

E

-

G

-

L

T

T

Q

T

K

R

E

E

M

I

T

D

E

T

L

L

T

M

R

N

K

K

G

S

G

G

L

V

V

L

S

G

Y

V

F

S

G

G

-

L

T

S

N

N

D
|
D

A

F

R
|
R

I

D

V

L

E

D

E

E

K

A

A

A

K

S

N

K

G

G

D

N

E

R

Q

K

A

A

Q

E

L

L

I

A

L

L

R

E

A

I

M

F

A

A

Y

Y

Q

K

I

V

A

K

K

K

T

F

I

I

G

G

E

E

L

Y

A

S

T

A

V

V

L

L

C

N

G

G

K

-

V

A

D

D

A

A

I

V

L

F

T

T

G

A

G
|
G

L
x
I

A

G

active site: R241 (= R217)
binding adenosine monophosphate: D283 (= D256), R285 (= R258), G331 (= G305), I332 (≠ L306)
binding trihydrogen thiodiphosphate: G212 (= G191), R241 (= R217)

Sites not aligning to the query:

active site: 7, 91, 180, 384
binding adenosine monophosphate: 335, 336
binding trihydrogen thiodiphosphate: 180

4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwsA

query
sites
4fwsA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E
x
T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K
|
K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding cytidine-5'-triphosphate: G202 (= G189), N203 (≠ G190), G204 (= G191), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)
binding 1,2-ethanediol: N203 (≠ G190), T232 (≠ E216), R233 (= R217), K262 (= K244)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 21, 24, 115

4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwrA

query
sites
4fwrA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G

G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding cytidine-5'-monophosphate: G202 (= G189), N203 (≠ G190), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378

4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwqA

query
sites
4fwqA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G

G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E
|
E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding guanosine-5'-triphosphate: H172 (= H158), N203 (≠ G190), G204 (= G191), D275 (= D256), L276 (≠ A257), R277 (= R258), E280 (= E261), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378

4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwpA

query
sites
4fwpA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G

G

G
x
N

G

G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K
|
K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E
|
E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S
|
S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding 1,2-ethanediol: K262 (= K244)
binding guanosine-5'-diphosphate: N203 (≠ G190), D275 (= D256), L276 (≠ A257), R277 (= R258), E280 (= E261), G323 (= G305), I324 (≠ L306), N327 (≠ Y308), S328 (= S309)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 11, 115

4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwoA

query
sites
4fwoA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G

G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E
|
E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding guanosine-5'-monophosphate: G202 (= G189), N203 (≠ G190), D275 (= D256), L276 (≠ A257), R277 (= R258), E280 (= E261), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 100, 104

4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwnA

query
sites
4fwnA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H
|
H

I

L

G

G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding adenosine-5'-tetraphosphate: H172 (= H158), H200 (= H187), N203 (≠ G190), G204 (= G191), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378

4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwmA

query
sites
4fwmA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H
|
H

I

L

G

G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding adenosine-5'-triphosphate: H172 (= H158), H200 (= H187), N203 (≠ G190), G204 (= G191), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)
binding 1,2-ethanediol: H172 (= H158), R233 (= R217)

Sites not aligning to the query:

active site: 8, 83, 378

4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 4fwkA

query
sites
4fwkA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G

G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding adenosine monophosphate: G202 (= G189), N203 (≠ G190), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378
binding 1,2-ethanediol: 103, 104, 107

2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 2e1zA

query
sites
2e1zA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D
x
P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding bis(adenosine)-5'-tetraphosphate: G202 (= G189), N203 (≠ G190), G204 (= G191), P224 (≠ D208), R233 (= R217), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378
binding bis(adenosine)-5'-tetraphosphate: 8, 83, 115

1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 1x3nA

query
sites
1x3nA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G
|
G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G

G

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G189), N203 (≠ G190), G204 (= G191), D275 (= D256), L276 (≠ A257), R277 (= R258), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378

1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
32% identity, 45% coverage: 158:323/365 of query aligns to 172:342/394 of 1x3mA

query
sites
1x3mA

H
|
H

V

G

L

T

N

S

M

H

R

R

A

Y

V

V

S

S

M

R

K

R

I

-

A

A

D

Y

E

E

Q

L

L

L

G

D

K

L

P

D

Y

E

E

K

E

D

S

S

R

G

L

L

V

I

V

V

A

A

H

H

I

L

G
|
G

G
x
N

G

G

S

A

S

S

A

I

S

C

A

A

H

V

R

R

N

N

G

G

R

Q

I

S

I

V

D

D

L

T

-

S

-

M

-

G

V

M

S

T

D

P

D

L

E

E

G

G

L

L

F

M

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

V

P

D

L

F

K

G

E

A

I

M

I

A

P

W

M

I

C

A

F

-

Q

K

R

E

T

T

Q

G

K

Q

E

T

M

L

T

S

E

D

L

L

T

E

R

R

-

V

-

N

K

K

K

E

G

S

G

G

L

L

V

L

S

G

Y

I

F

S

G

G

-

L

T

S

N

S

D
|
D

A
x
L

R
|
R

I

V

V

L

E

E

E

K

K

A

A

W

K

H

N

E

G

G

D

H

E

E

Q

R

A

A

Q

R

L

L

I

A

L

I

R

K

A

T

M

F

A

V

Y

H

Q

R

I

I

A

A

K

R

T

H

I

I

G

A

E

G

L

H

A

A

T

A

V

S

L

L

C

H

G

-

K

R

V

L

D

D

A

G

I

I

L

F

T

T

G
|
G

L
x
I

A

G

-

E

Y
x
N

S

S

E

V

Y

L

V

I

M

R

N

Q

M

L

V

V

K

I

E

E

R

H

V

L

S

G

F

V

L

L

active site: H172 (= H158), R233 (= R217)
binding adenosine-5'-diphosphate: G202 (= G189), N203 (≠ G190), D275 (= D256), L276 (≠ A257), R277 (= R258), G322 (= G304), G323 (= G305), I324 (≠ L306), N327 (≠ Y308)

Sites not aligning to the query:

active site: 8, 83, 378

7fj9A Kpacka (pduw) with amppnp complex structure
31% identity, 37% coverage: 172:306/365 of query aligns to 191:330/395 of 7fj9A

query
sites
7fj9A

E

E

Q

K

L

L

G

G

K

V

P

P

Y

L

E

S

E

A

S

L

R

R

L

V

V

V

V

S

A

C

H

H

I

L

G
|
G

G
x
N

G
|
G

S

C

S

S

A

V

S

C

A

A

H

I

R

K

N

G

G

G

R

Q

I

S

I

V

D

N

L

T

-

S

-

M

-

G

V

F

S

T

D

P

D

Q

E

S

G

G

L

V

F

M

S

M

T

G

E

T

R

R

S

S

G

G

G

D

L

I

P

D

L

-

K

P

E

S

I

I

I

L

P

P

M

W

C

L

F

V

Q

E

-

K

-

E

-

G

R

K

T

S

Q

A

K

Q

E

Q

M

L

T

S

E

Q

L

L

T

L

R

N

K

N

K

E

G

S

G

G

L

L

V

L

S

G

Y

V

F

S

G

G

-

V

T

S

N

S

D
|
D

A
x
Y

R
|
R

I

D

V

V

E

E

E

Q

K

A

A

A

K

D

N

A

G

G

D

N

E

E

Q

R

A

A

Q

A

L

L

I

A

L

L

R

S

A

L

M

F

A

A

Y

E

Q

R

I

I

A

R

K

A

T

T

I

I

G

G

E

S

L

Y

A

I

T

M

V

Q

L

M

C

-

G

G

K

G

V

L

D

D

A

A

I

L

I

I

L

F

T

T

G

G

G
|
G

L
x
I

binding phosphoaminophosphonic acid-adenylate ester: G208 (= G189), N209 (≠ G190), G210 (= G191), D281 (= D256), Y282 (≠ A257), R283 (= R258), G329 (= G305), I330 (≠ L306)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 178, 333

7fj8A Kpacka (pduw) with amp complex structure
31% identity, 37% coverage: 172:306/365 of query aligns to 191:330/395 of 7fj8A

query
sites
7fj8A

E

E

Q

K

L

L

G

G

K

V

P

P

Y

L

E

S

E

A

S

L

R

R

L

V

V

V

V

S

A

C

H

H

I

L

G
|
G

G
x
N

G

G

S

C

S

S

A

V

S

C

A

A

H

I

R

K

N

G

G

G

R

Q

I

S

I

V

D

N

L

T

-

S

-

M

-

G

V

F

S

T

D

P

D

Q

E

S

G

G

L

V

F

M

S

M

T

G

E

T

R

R

S

S

G

G

G

D

L

I

P

D

L

-

K

P

E

S

I

I

I

L

P

P

M

W

C

L

F

V

Q

E

-

K

-

E

-

G

R

K

T

S

Q

A

K

Q

E

Q

M

L

T

S

E

Q

L

L

T

L

R

N

K

N

K

E

G

S

G

G

L

L

V

L

S

G

Y

V

F

S

G

G

-

V

T

S

N

S

D
|
D

A
x
Y

R
|
R

I

D

V

V

E

E

E

Q

K

A

A

A

K

D

N

A

G

G

D

N

E

E

Q

R

A

A

Q

A

L

L

I

A

L

L

R

S

A

L

M

F

A

A

Y

E

Q

R

I

I

A

R

K

A

T

T

I

I

G

G

E

S

L

Y

A

I

T

M

V

Q

L

M

C

-

G

G

K

G

V

L

D

D

A

A

I

L

I

I

L

F

T

T

G

G

G
|
G

L
x
I

binding adenosine monophosphate: G208 (= G189), N209 (≠ G190), D281 (= D256), Y282 (≠ A257), R283 (= R258), G329 (= G305), I330 (≠ L306)

Sites not aligning to the query:

binding adenosine monophosphate: 333

4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
29% identity, 47% coverage: 174:345/365 of query aligns to 178:351/381 of 4iz9A

query
sites
4iz9A

L

L

G

D

K

R

P

P

Y

L

E

R

E

G

S

L

R

K

L

Q

V

I

V

V

A

L

H

H

I

L

G
|
G

G
x
N

G

G

S

C

S

S

A

A

S

S

A

A

H

I

R

A

N

G

G

T

R

R

I

P

I

L

D

D

L

T

-

S

-

M

-

G

V

L

S

T

D

P

D

L

E

E

G

G

L

L

F

V

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

I

P

D

L

P

K

S

E

V

I

V

I

S

P

Y

M

L

C

C

F

H

Q

T

R

A

T

G

Q

M

-

G

-

V

K

D

E

D

M

V

T

E

E

S

L

M

T

L

R

N

K

H

K

R

G

S

G

G

L

V

V

V

S

G

Y

L

F

S

G

G

T

V

N

R

D
|
D

A
x
F

R
|
R

I

R

V

L

E

R

E

E

K

L

A

I

K

E

N

S

G

G

D

D

E

G

Q

A

A

A

Q

Q

L

L

I

A

L

Y

R

S

A

V

M

F

A

T

Y

H

Q

R

I

L

A

R

K

K

T

Y

I

I

G

G

E

A

L

Y

A

L

T

A

V

V

L

L

C

-

G

G

K

H

V

T

D

D

A

V

I

I

I

S

L

F

T

T

G

A

G
|
G

L
x
I

A

G

Y

E

S
x
N

E
x
D

Y

A

V

A

M

V

N

R

M

-

V

-

K

R

E

D

R

A

V

V

S

S

F

G

L

M

A

E

P

E

V

L

H

G

I

I

V

V

P

L

G

D

E

E

E

R

L

N

L

L

A

P

L

G

A

A

K

K

G

G

A

A

R

R

R

Q

V

I

active site: R224 (= R217)
binding diphosphomethylphosphonic acid adenosyl ester: G193 (= G189), N194 (≠ G190), D265 (= D256), F266 (≠ A257), R267 (= R258), G313 (= G305), I314 (≠ L306), N317 (≠ S309), D318 (≠ E310)

Sites not aligning to the query:

active site: 10, 74, 163, 367
binding diphosphomethylphosphonic acid adenosyl ester: 17

4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
31% identity, 36% coverage: 174:306/365 of query aligns to 176:312/376 of 4ijnA

query
sites
4ijnA

L

L

G

D

K

R

P

P

Y

L

E

E

E

S

S

L

R

N

L

Q

V

I

V

V

A

L

H

H

I

L

G
|
G

G
x
N

G

G

S

A

S

S

A

A

S

S

A

A

H

V

R

A

N

G

G

G

R

K

I

A

I

V

D

D

L

T

-

S

-

M

-

G

V

L

S

T

D

P

D

M

E

E

G

G

L

L

F

V

S

M

T

G

E

T

R
|
R

S

S

G

G

G

D

L

I

P

D

L

P

K

G

E

V

I

I

I

-

P

-

M

M

C

Y

F

L

Q

W

R

R

T

T

Q

A

-

G

-

M

-

S

-

V

K

D

E

D

M

I

T

E

E

S

L

M

T

L

R

N

K

R

G

S

G

G

L

V

V

L

S

G

Y

L

F

G

G

G

T

A

N

S

D
|
D

A
x
F

R
|
R

I

K

V

L

E

R

E

E

K

L

A

I

K

E

N

S

G

G

D

D

E

E

Q

H

A

A

Q

K

L

L

I

A

L

Y

R

D

A

V

M

Y

A

I

Y

H

Q

R

I

L

A

R

K

K

T

Y

I

I

G

G

E

A

L

Y

A

M

T

A

V

V

L

L

C

-

G

G

K

R

V

T

D

D

A

V

I

I

I

S

L

F

T

T

G

A

G
|
G

L
x
V

active site: R222 (= R217)
binding adenosine monophosphate: G191 (= G189), N192 (≠ G190), D263 (= D256), F264 (≠ A257), R265 (= R258), G311 (= G305), V312 (≠ L306)

Sites not aligning to the query:

active site: 8, 72, 161, 365
binding adenosine monophosphate: 315, 316

Query Sequence

>WP_036833137.1 NCBI__GCF_000775615.1:WP_036833137.1
MKHRVLAINPGATSTKVACYENDELTFKKEFTYHYETISQYDSIMQQYHLRYQDIATFLQ
EMDMKIGSFDAVVGRGGLLPPVKAGAYRVNEAMLDCLEHRPVLQHASNLGASLAKGIAEV
FGTEDCQAFIYDPVTVDQMEDVARISGSASIERKSIGHVLNMRAVSMKIADEQLGKPYEE
SRLVVAHIGGGSSASAHRNGRIIDLVSDDEGLFSTERSGGLPLKEIIPMCFQRTQKEMTE
LTRKKGGLVSYFGTNDARIVEEKAKNGDEQAQLILRAMAYQIAKTIGELATVLCGKVDAI
ILTGGLAYSEYVMNMVKERVSFLAPVHIVPGEEELLALAKGARRVLTGQEHAHTFIENEA
IVASK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory