SitesBLAST

R257 (= R261) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ I285) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ S340) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ V345) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (≠ F372) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

8j74A Human high-affinity choline transporter cht1 in the hc-3-bound outward-facing open conformation, dimeric state (see paper)
24% identity, 90% coverage: 7:434/478 of query aligns to 7:441/515 of 8j74A

query
sites
8j74A

I

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binding Lauryl Maltose Neopentyl Glycol: F203 (≠ T205), S206 (≠ A208), H207 (≠ G209), I213 (= I215), G214 (≠ D218), F215 (≠ L219), H219 (≠ N223), C326 (≠ L318), P327 (= P319)
binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W60 (≠ Y57), Y89 (= Y89), W139 (≠ I133), L245 (= L247), W252 (≠ H257), W404 (≠ G402), Y405 (≠ G403), S408 (≠ A406)

9bimA Cryo-em structure of human cht1 in the hc-3 bound outward-facing state
24% identity, 90% coverage: 7:434/478 of query aligns to 8:440/516 of 9bimA

query
sites
9bimA

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A

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x
Y

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x
S

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C

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K

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N

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L

binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W59 (≠ Y57), Y77 (≠ L75), Y88 (= Y89), W138 (≠ I133), W251 (≠ H257), Y404 (≠ G403), S407 (≠ A406)

8zqpA High affinity choline transporter 1
24% identity, 90% coverage: 7:434/478 of query aligns to 6:440/470 of 8zqpA

query
sites
8zqpA

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x
G

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Y

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-

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A

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F

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C

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A

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I

V

M

M

S

S

T

S

V

A

D

D

S

S

L

S

L

I

L

L

I

S

V

A

S

S

A

M

I

F

V

A

K

R

D

N

V

I

Y

Y

I

Q

N

L

Y

S

V

F

N

R

P

Q

S

N

A

A

T

S

N

D

E

K

K

E

I

I

K

-

L

-

S

-

F

-

G

-

V

-

T

V

A

W

T

V

L

M

G

R

I

I

L

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V

V

F

F

L

V

F

F

A

G

L

A

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A

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A

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M

-

A

L

L

I

L

W

T

L

K

N

T

L

V

Y

Y

A

G

F

L

G

W

G

Y

L

L

E

S

A

S

A

D

F

L

-

V

-

Y

-

I

-

V

I

I

W

F

P

P

V

Q

V

L

L

L

G

C

L

V

Y

L

W

F

S

V

K

K

G

G

-

T

N

N

Q

T

Y

Y

G

G

A

A

I

V

A

A

S

G

M

Y

V

V

T

S

G

G

I

L

binding 4-methoxy-3-[(1-methylpiperidin-4-yl)oxy]-N-{[3-(propan-2-yl)-1,2-oxazol-5-yl]methyl}benzamide: G63 (≠ S61), Y88 (= Y89)

Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 5 papers)
24% identity, 90% coverage: 7:434/478 of query aligns to 9:443/580 of Q9GZV3

query
sites
Q9GZV3

I

I

V

A

L

I

F

I

L

V

F

F

L

Y

C

L

I

L

I

I

F

L

A

L

V

V

G

G

F

I

W

W

A

A

-

A

-

W

-

R

S

T

K

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S

N

V

S

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G

T

S

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A

E

E

S

E

F

R

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S

Q

E

E

A

Y

I

F

I

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V

G

G

R

R

Q
x
D

L

I

G

G

G

L

F

L

I

V

L

G

A

G

M

F

T

T

M

M

T

T

A

A

T

T

Y

W

G

V

S

G

A
x
G

S

G

S

Y

F

I

I

N

G

G

G

T

P

A

G

E

L

A

A

V

Y

Y

N

V

Q

P

G

G

L

Y

G

G

W

-

V

-

L

-

L

L

A

A

M

W

S

A

Q

Q

V

A

V

P

T
x
I

G

G

Y

Y

F

S

V

L

L

S

M

L

V

I

L

L

G

G

K

G

R

L

F

F

-

F

A

A

I

K

I
x
P

A

M

R

R

K

S

Y

K

N

G

A
x
Y

I

V

T

T

L

M

I

L

D

D

F

P

L

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Q

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Q

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I

Y

Y

N

G

N

K

N

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K

M

W

G

-

G

-

L

-

F

-

I

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P

V

A

L

L

L

M

G

G

A

E

F

M

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F

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V

A

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S

A

A

M

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A

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A

A

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-

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-

A

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R

-

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-

I

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S

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T

H

S

I

A

S

L

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A

A

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A

I

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A

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Y
|
Y

V

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V

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I

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F

L

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Y

A

S

V

V

A

A

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T

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Q

G

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V

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C

M

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-

L

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I

W

L

I

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S

I

V

A

P

T

F

I

A

I

L

A

S

G

H

G

P

G

A

V

V

N

A

N

D

I

I

M

-

E

-

D

G

L

F

V

T

R

A

L

V

N

H

P

A

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Y

I

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K

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P

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W

G

L

A

G

N

T

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A

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Y

Y

-

S

-

W

V

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S

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F

F

W

L

I

L

L

L

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M

G

-

V

-

G

-

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G

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I

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P

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W

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A

V

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A

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Y

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S

K

S

A

A

M

T

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Y

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A

A

Q

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F

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L

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G

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M

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x
I

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A

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A

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A

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x
V

M

M

S

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S

V

A

D

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L

S

L

I

L

L

I

S

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A

S

S

A

M

I

F

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A

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x
R

D

N

V

I

Y

Y

I

Q

N

L

Y

S

V

F

N

R

P

Q

S

N

A

A

T

S

N

D

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K

K

E

I

I

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-

L

-

S

-

F

-

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-

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T

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G

R

I

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V

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F

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F

F

A

G

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A

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P

A

P

T

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A

L

M

-

A

L

L

I

L

W

T

L

K

N

T

L

V

Y

Y

A

G

F

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G

W

G

Y

L

L

E

S

A

S

A

D

F

L

-

V

-

Y

-

I

-

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I

I

W
x
F

P

P

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Q

V

L

L

L

G

C

L

V

Y

L

W

F

S

V

K

K

G

G

-

T

N

N

Q

T

Y

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G

G

A

A

I

V

A

A

S

G

M

Y

V

V

T

S

G

G

I

L

D48 (≠ Q43) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
G65 (≠ A60) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
I89 (≠ T87) to V: 40% reduction in choline transmembrane transporter activity; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Decreased choline transmembrane transporter activity, only 20% of wild-type choline uptake activity.
P105 (≠ I102) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
Y111 (≠ A108) to H: in CMS20; no effect on localization at plasma membrane
Y175 (= Y174) to C: in CMS20; uncertain significance; dbSNP:rs1331713195
I291 (≠ M289) to T: in CMS20; uncertain significance; dbSNP:rs375397889
V344 (≠ I334) to L: in CMS20; uncertain significance
R361 (≠ K351) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110
F418 (≠ W410) to V: in CMS20; uncertain significance

Sites not aligning to the query:

446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
451 E→Q: Decreased choline transmembrane transporter activity, only 5% of wild-type choline uptake activity.
527:532 Dileucine-like motif
530 I→A: No change in protein internalization. No change in choline transmembrane transporter activity.
531 L→A: Loss of protein internalization to vesicular structures in neurons. Increased choline transmembrane transporter activity.
531:532 LV→AA: Decreased protein internalization; when associated with V-538. Increased choline transmembrane transporter activity; when associated with V-538.
532 V→A: Decreased protein internalization. Increased choline transmembrane transporter activity.
538 K→V: Decreased protein internalization; when associated with 531-L-V-532. Increased choline transmembrane transporter activity; when associated with 531-L-V-532.

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
22% identity, 76% coverage: 2:365/478 of query aligns to 28:421/659 of Q9NY91

query
sites
Q9NY91

N

D

I

I

T

S

V

V

T

I

I

V

V

I

L

Y

F

F

L

L

F

-

L

-

C

-

I

V

F

M

A

A

V

V

G

G

F

L

W

W

A

A

S

M

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-

S

-

V

L

Q

K

T

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S

N

E

R

S

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G

E

G

Y

F

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F

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L

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A

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G

A

G

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F

W

I

P

L

M

A

G

M

A

T

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M

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T

F

A

A

T

S

Y

N

G

I

S

G

A

S

S

N

S

H

F

Y

I

V

G

G

G

L

P

A

G

G

L

T

A

G

Y

A

N

A

Q

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G

G

L

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G

A

W

T

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V

L

T

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F

A

E

M

W

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T

Q

S

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S

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V

T

-

G

-

Y

-

F

-

V

M

L

L

M

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L

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G

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K

W

R

I

F

F

A

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I

I

A

Y

R

I

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K

Y

S

N

G

A

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I

M

T

T

L

M

I

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D

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F

Y

L

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K

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N

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A

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A

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G

A

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F

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L

A

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G

L

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Y

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S

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A

A

L

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F

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L

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F

L

A

L

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A

A

M

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T

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A

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Y

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T

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T

G

G

F

L

K

A

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V

A

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D

D

T

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L

Q

Q

G

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V

I

M

M

F

L

F

I

G

G

T

S

L

F

I

I

L

L

L

M

I

G

A

F

T

A

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F

I

N

A

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G

V

G

G

V

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N

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N

S

I

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L

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V

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N

L

A

N

T

P

P

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S

M

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V

T

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P

-

Y

-

G

G

A

D

N

N

G

L

D

T

L

I

S

S

P

A

A

S

-

C

-

Y

-

T

-

P

Y

R

V

A

S

D

S

S

F

F

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H

I

I

L

F

V

R

G

D

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A

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-

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-

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-

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I

-

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-

W

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G

G

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F

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M

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I

V

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A

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L

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W

-

Y

-

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-

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N

-

Q

-

V

-

I

-

V

-

Q

R

R

A

C

M

L

S

C

Y

G

K

K

S

D

S

M

K

S

A

H

M

V

H

K

R

A

A

A

I

C

I

I

I

M

G

C

T

A

I

Y

V

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V

K

G

L

V

L

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P

M

M

L

F

N

L

M

M

H

V

L

M

V

P

G

G

V

M

F

I

A

S

K

R

-

I

-

L

-

Y

-

T

-

D

-

M

-

V

-

A

P

C

I

V

L

V

P

P

-

S

-

E

-

C

-

V

-

K

-

H

-

C

-

G

-

V

N

D

I

V

D

G

N

C

A

T

D

N

M

Y

V

A

M

Y

P

P

T

T

I

M

A

V

Q

L

H

E

V

L

L

M

P

P

P

Q

W

G

L

L

A

R

G

G

I

L

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M

L

L

A

S

A

V

P

M

M

L

A

A

A

S

I

L

M

M

S

S

T

S

V

L

D

T

S

S

L

I

L

F

L

N

I

S

V

A

S

S

S

T

A

L

I

F

V

T

K

I

D

D

V

L

Y

Y

I

T

N

K

Y

M

V

R

N

K

P

Q

S

A

A

S

T

E

N

K

E

E

Sites not aligning to the query:

457 E→Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

8hezA Structure of human sglt2-map17 complex with dapagliflozin (see paper)
23% identity, 77% coverage: 2:367/478 of query aligns to 2:402/582 of 8hezA

query
sites
8hezA

N

N

I

P

T

A

V

D

T

I

I

L

V

V

L

I

F

A

L

A

F

Y

L

F

C

L

I

L

I

V

F

I

A

G

V

V

G

G

F

L

W

W

A

S

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M

K

-

S

-

V

C

Q

R

T

T

S

N

E

R

S

G

F

T

L

V

Q

G

E

G

Y

Y

F

F

L

L

G

A

G

G

R

R

Q

S

L

M

G

V

G

W

F

W

I

P

L

V

A

G

M

A

T

S

M

L

T

F

A
|
A

T

S

Y
x
N

G
x
I

S
x
G

A

S

S
x
G

S
x
H

F

F

I

V

G
|
G

G
x
L

P

A

G

G

L
x
T

A

G

Y

A

N

A

Q

S

G

G

L

L

G

A

W

-

V

-

L

-

A

-

M

-

S

-

Q

-

V

-

V

V

T

A

G

G

Y
x
F

-
x
E

-

W

-

N

-

A

-

L

F

F

V

V

L

V

M

L

V

L

L

L

G

G

K

W

R

L

F

F

A

A

I

P

I

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A

Y

R

L

K

T

Y

A

N

G

A

V

I

I

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T

L

M

I

P

D

Q

F

Y

L

L

K

R

V

K

R

R

Y

F

N

G

N

R

K

R

W

I

V

R

V

L

L

Y

L

L

G

S

A

V

F

L

T

S

I

L

V

F

V

L

F

Y

L

I

F

F

S

T

A

K

M

I

A

S

A

V

Q

D

W

M

V

F

G

S

G

G

A

A

R

V

L

F

I

I

E

Q

S

Q

L

A

T

L

G

G

L

W

S

N

-

I

Y

Y

T

A

S

S

A

V

L

I

F

A

L

L

F

L

A

G

I

I

A

-

V

T

L

M

V

I

Y

Y

V

T

V

V

I

T

G

G

F

L

K

A

A

A

V

L

A

M

L

Y

T

T

D

D

T

T

V

V

Q

Q

G

T

V

F

V

V

M

I

F

L

F

G

G

G

T

A

L

C

I

I

L

L

L

M

I

G

A

Y

T

A

I

F

I

H

A

E

G

V

G

G

-

Y

-

S

-

G

-

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

-

S

-

L

-

T

-

V

-

S

-

E

-

D

-

P

-

A

V

V

N

G

N

N

I

I

M

S

E

S

D

F

L

C

V

Y

R

R

L

P

N

R

P

P

N

D

-

S

-

Y

M

H

I

L

T

L

P

R

Y

H

G

P

A

V

N

T

G

G

D

D

L

L

S

P

-

W

P

P

A

A

Y

L

V

L

S

L

S

G

F

L

W

T

I

I

L
x
V

V
x
S

G

G

V

W

G

Y

V
x
W

V

C

G

S

L

D

P

Q

H

V

V

I

T

V

V

Q

R

R

A

C

M

L

S

A

Y

G

K

K

S

S

S

L

K

T

A

H

M

I

H

K

R

A

A

G

I

C

I

I

I

L

G

C

T

G

I

Y

V

L

V
x
K

G

L

V

T

I

P

M

M

L

F

N

L

M

M

H

V

L

M

V

P

G

G

V

M

F

I

A

S

K

R

P

I

I

L

L

Y

P

P

N

-

I

-

D

D

N

E

A

V

D

A

M

C

V

V

M

V

P

P

T

E

I

V

A

C

Q

R

H

R

V

V

-

C

-

G

-

T

-

E

-

V

-

G

-

C

-

S

-

N

-

I

-

A

-

Y

-

P

-

R

-

L

-

V

-

K

-

L

L

M

P

P

P

N

W

G

L

L

A

R

G

G

I

L

V

M

L

L

A

A

A

V

P

M

M

L

A

A

A
|
A

I

L

M

M

S
|
S

T
x
S

V

L

D

A

S

S

L

I

L

F

L

N

I

S

V

S

S

S

S

T

A

L

I

F

V

T

K

M

D

D

V

I

Y

Y

I

T

N

R

Y

-

V

L

N

R

P

P

S

R

A

A

T

G

N

D

E

R

K

E

I

L

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y57), G59 (≠ S61), H60 (≠ S62), G63 (= G65), L64 (≠ G66), T67 (≠ L69), F78 (≠ Y89), E79 (vs. gap), V266 (≠ L247), S267 (≠ V248), W271 (≠ V252), K301 (≠ V282)
binding sodium ion: A53 (= A55), I56 (≠ G58), G57 (≠ S59), A369 (= A333), S372 (= S336), S373 (≠ T337)

Sites not aligning to the query:

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 433, 437
binding : 568, 571, 572, 575, 576, 579, 580

Q8N695 Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8 from Homo sapiens (Human) (see 3 papers)
24% identity, 76% coverage: 22:382/478 of query aligns to 34:396/610 of Q8N695

query
sites
Q8N695

W

F

A

A

S

G

K

G

S

G

V

Q

Q

Q

T

T

S

S

E

K

S

D

F

F

L

L

Q

-

E

-

Y

-

F

-

L

M

G

G

R

R

Q

R

L

M

G

T

G

A

F

V

I

P

L

V

A

A

M

L

T

S

M

L

T

T

A

A

T

S

Y

F

G

M

S

S

A

A

S

V

S

T

F

V

I

L

G

G

G

T

P

P

G

S

L

E

A

V

Y

Y

N

R

Q

F

G

G

L

A

G

I

W

F

V

S

L

I

L

F

A

A

M

F

S

-

Q

-

V

-

T

T

G

Y

Y

F

F

F

V

V

L

V

M

V

V

I

L

S

G

A

K

E

R

V

F

F

A

L

I

P

I

V

A

F

R

Y

K

K

Y

L

N

G

A

I

I

T

T

S

L

T

I

Y

D

E

F

Y

L

L

K

E

V

L

R

R

Y

F

N

N

N

-

K

K

W

C

V

V

V

R

L

L

L

C

G

G

A

T

-

V

-

L

F

F

T

I

I

V

V

Q

V

T

F

I

L

L

F

Y

S

T

A

G

M

I

A

V

A

I

Q

-

W

-

V

Y

G

A

G

P

A

A

R

L

L

A

I

L

E

N

S

Q

L

V

T

T

G

G

L

F

S

D

Y

L

T

W

S

G

A

A

L

V

F

V

L

A

F

T

A

G

I

V

A

V

V

C

L

T

V

F

Y

Y

V

C

V

T

I

L

G

G

F

L

K

K

A

A

V

V

A

I

L

W

T

T

D

D

T

V

V

F

Q

Q

G
x
V

V

G

V

I

M

M

F

V

F

A

G

G

T

F

L

A

I

S

L

V

L

I

I

I

A

Q

T

A

I

V

A

M

G

Q

G

G

V

I

N

S

N

T

I

I

M

L

E

N

D

D

L

A

V

Y

-

D

-

G

R

R

L

L

N

N

P

F

N

W

M

N

I

F

T

N

P

P

Y

-

G

-

A

-

N

-

G

-

D

-

L

-

S

N

P

P

A

L

Y

Q

V

R

S

H

S

T

F

F

W

W

-

T

I

I

L

I

V

I

G

G

-

G

-

T

-
x
F

-

T

-

W

V

T

G

S

V

I

V

Y

G

G

L

V

P

N

H

Q

V

S

T

Q

V

V

-

Q

R

R

A

Y

M

I

S

S

Y

C

K

K

S

S

S

R

K

F

A

Q

M

A

H

K

R

L

A

S

I

L

I

Y

I

I

G

N

T

L

I

V

V

G

V

L

G

W

V

A

I

I

M

L

L

T

N

C

M

S

H

V

L

F

V

C

G

G

V

L

F

A

A

L

K

Y

P

S

I

R

L

Y

P

H

N

D

I

C

D

D

-

P

-

W

-

T

-

A

-

K

-

V

-

S

N

A

A

P

D

D

M

Q

V

L

M

M

P

P

T

Y

I

L

A

V

Q

L

H

D

V

I

L

L

P

Q

-

D

-

Y

P

P

W

G

L

L

A

P

G

G

I

L

V

F

L

V

A

A

A

C

P

A

M

Y

A

S

A

G

I

T

M

L

S

S

T

T

V

V

D

S

S

S

L

S

L

I

L

N

I

A

V

L

S

A

A

V

I

T

V

V

K

E

D

D

V

L

Y

I

I

K

N

P

Y

Y

V

F

N

R

P

-

S

S

A

L

T

S

N

E

E

R

K

S

I

L

K

S

K

W

L

I

S

S

F

Q

G

G

V

M

T

S

A

V

T

V

L

Y

G

G

I

A

L

L

V

C

V193 (≠ G191) to I: in dbSNP:rs1709189
F251 (vs. gap) to V: in dbSNP:rs11834933

Sites not aligning to the query:

608 T→A: Loss of interaction with PDZK1.
608:610 PDZ-binding
610 L→A: Loss of interaction with PDZK1.

8hdhA Structure of human sglt2-map17 complex with canagliflozin (see paper)
23% identity, 77% coverage: 2:367/478 of query aligns to 2:402/586 of 8hdhA

query
sites
8hdhA

N

N

I

P

T

A

V

D

T

I

I

L

V

V

L

I

F

A

L

A

F

Y

L

F

C

L

I

L

I

V

F

I

A

G

V

V

G

G

F

L

W

W

A

S

S

M

K

-

S

-

V

C

Q

R

T

T

S

N

E

R

S

G

F

T

L

V

Q

G

E

G

Y

Y

F

F

L

L

G

A

G

G

R

R

Q

S

L

M

G

V

G

W

F

W

I

P

L

V

A

G

M

A

T

S

M

L

T

F

A
|
A

T
x
S

Y
x
N

G
x
I

S
x
G

A

S

S
x
G

S
x
H

F

F

I

V

G
|
G

G
x
L

P

A

G

G

L

T

A

G

Y

A

N

A

Q

S

G

G

L

L

G

A

W

-

V

-

L

-

A

-

M

-

S

-

Q

-

V

-

V

V

T

A

G

G

Y
x
F

-
x
E

-

W

-

N

-

A

-

L

F

F

V

V

L

V

M

L

V

L

L

L

G

G

K

W

R

L

F

F

A

A

I

P

I

V

A

Y

R

L

K

T

Y

A

N

G

A

V

I

I

T

T

L

M

I

P

D

Q

F

Y

L

L

K

R

V

K

R

R

Y

F

N

G

N

R

K

R

W

I

V

R

V

L

L

Y

L

L

G

S

A

V

F

L

T

S

I

L

V

F

V

L

F

Y

L

I

F

F

S

T

A

K

M

I

A

S

A

V

Q

D

W

M

V

F

G

S

G

G

A

A

R

V

L

F

I

I

E

Q

S

Q

L

A

T

L

G

G

L

W

S

N

-

I

Y

Y

T

A

S

S

A

V

L

I

F

A

L

L

F

L

A

G

I

I

A

-

V

T

L

M

V

I

Y

Y

V

T

V

V

I

T

G

G

F

L

K

A

A

A

V

L

A

M

L

Y

T

T

D

D

T

T

V

V

Q

Q

G

T

V

F

V

V

M

I

F

L

F

G

G

G

T

A

L

C

I

I

L

L

L

M

I

G

A

Y

T

A

I

F

I

H

A

E

G

V

G

G

-

Y

-

S

-

G

-

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

-

S

-

L

-

T

-

V

-

S

-

E

-

D

-

P

-

A

V

V

N

G

N

N

I

I

M

S

E

S

D

F

L

C

V

Y

R

R

L

P

N

R

P

P

N

D

-

S

-

Y

M

H

I

L

T

L

P

R

Y

H

G

P

A

V

N

T

G

G

D

D

L

L

S

P

-

W

P

P

A

A

Y

L

V

L

S

L

S

G

F

L

W

T

I

I

L

V

V
x
S

G

G

V

W

G

Y

V
x
W

V

C

G

S

L

D

P

Q

H

V

V

I

T

V

V

Q

R

R

A

C

M

L

S

A

Y

G

K

K

S

S

S

L

K

T

A

H

M

I

H

K

R

A

A

G

I

C

I

I

I

L

G

C

T

G

I

Y

V

L

V

K

G

L

V

T

I

P

M

M

L

F

N

L

M

M

H

V

L

M

V

P

G

G

V

M

F

I

A

S

K

R

P

I

I

L

L

Y

P

P

N

-

I

-

D

D

N

E

A

V

D

A

M

C

V

V

M

V

P

P

T

E

I

V

A

C

Q

R

H

R

V

V

-

C

-

G

-

T

-

E

-

V

-

G

-

C

-

S

-

N

-

I

-

A

-

Y

-

P

-

R

-

L

-

V

-

K

-

L

L

M

P

P

P

N

W

G

L

L

A

R

G

G

I

L

V

M

L

L

A

A

A

V

P

M

M

L

A

A

A
|
A

I

L

M

M

S
|
S

T
x
S

V

L

D

A

S

S

L

I

L

F

L

N

I

S

V

S

S

S

S

T

A

L

I

F

V

T

K

M

D

D

V

I

Y

Y

I

T

N

R

Y

-

V

L

N

R

P

P

S

R

A

A

T

G

N

D

E

R

K

E

I

L

binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y57), G59 (≠ S61), H60 (≠ S62), G63 (= G65), L64 (≠ G66), F78 (≠ Y89), E79 (vs. gap), S267 (≠ V248), W271 (≠ V252)
binding sodium ion: A53 (= A55), S54 (≠ T56), I56 (≠ G58), G57 (≠ S59), A369 (= A333), S372 (= S336), S373 (≠ T337)

Sites not aligning to the query:

8hb0A Structure of human sglt2-map17 complex with ta1887 (see paper)
23% identity, 77% coverage: 2:367/478 of query aligns to 2:402/586 of 8hb0A

query
sites
8hb0A

N

N

I

P

T

A

V

D

T

I

I

L

V

V

L

I

F

A

L

A

F

Y

L

F

C

L

I

L

I

V

F

I

A

G

V

V

G

G

F

L

W

W

A

S

S

M

K

-

S

-

V

C

Q

R

T

T

S

N

E

R

S

G

F

T

L

V

Q

G

E

G

Y

Y

F

F

L

L

G

A

G

G

R

R

Q

S

L

M

G

V

G

W

F

W

I

P

L

V

A

G

M

A

T

S

M

L

T

F

A
|
A

T

S

Y
x
N

G
x
I

S
x
G

A

S

S

G

S
x
H

F

F

I

V

G
|
G

G
x
L

P

A

G

G

L
x
T

A

G

Y

A

N

A

Q

S

G

G

L

L

G

A

W

-

V

-

L

-

A

-

M

-

S

-

Q

-

V

-

V
|
V

T

A

G

G

Y
x
F

-
x
E

-

W

-

N

-

A

-

L

F

F

V

V

L

V

M

L

V

L

L

L

G

G

K

W

R

L

F

F

A

A

I

P

I

V

A

Y

R

L

K

T

Y

A

N

G

A

V

I

I

T

T

L

M

I

P

D

Q

F

Y

L

L

K

R

V

K

R

R

Y

F

N

G

N

R

K

R

W

I

V

R

V

L

L

Y

L

L

G

S

A

V

F

L

T

S

I

L

V

F

V

L

F

Y

L

I

F

F

S

T

A

K

M

I

A

S

A
x
V

Q

D

W

M

V

F

G

S

G

G

A

A

R

V

L

F

I

I

E

Q

S

Q

L

A

T

L

G

G

L

W

S

N

-

I

Y

Y

T

A

S

S

A

V

L

I

F

A

L

L

F

L

A

G

I

I

A

-

V

T

L

M

V

I

Y

Y

V

T

V

V

I

T

G

G

F

L

K

A

A

A

V

L

A

M

L

Y

T

T

D

D

T

T

V

V

Q

Q

G

T

V

F

V

V

M

I

F

L

F

G

G

G

T

A

L

C

I

I

L

L

L

M

I

G

A

Y

T

A

I

F

I

H

A

E

G

V

G

G

-

Y

-

S

-

G

-

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

-

S

-

L

-

T

-

V

-

S

-

E

-

D

-

P

-

A

V

V

N

G

N

N

I

I

M

S

E

S

D

F

L

C

V

Y

R

R

L

P

N

R

P

P

N

D

-

S

-

Y

M

H

I

L

T

L

P

R

Y

H

G

P

A

V

N

T

G

G

D

D

L

L

S

P

-

W

P

P

A

A

Y

L

V

L

S

L

S

G

F

L

W

T

I

I

L
x
V

V
x
S

G

G

V

W

G

Y

V
x
W

V

C

G

S

L

D

P

Q

H

V

V

I

T

V

V

Q

R

R

A

C

M

L

S

A

Y

G

K

K

S

S

S

L

K

T

A

H

M

I

H

K

R

A

A

G

I

C

I

I

I

L

G

C

T

G

I

Y

V

L

V

K

G

L

V

T

I

P

M

M

L

F

N

L

M

M

H

V

L

M

V

P

G

G

V

M

F

I

A

S

K

R

P

I

I

L

L

Y

P

P

N

-

I

-

D

D

N

E

A

V

D

A

M

C

V

V

M

V

P

P

T

E

I

V

A

C

Q

R

H

R

V

V

-

C

-

G

-

T

-

E

-

V

-

G

-

C

-

S

-

N

-

I

-

A

-

Y

-

P

-

R

-

L

-

V

-

K

-

L

L

M

P

P

P

N

W

G

L

L

A

R

G

G

I

L

V

M

L

L

A

A

A

V

P

M

M

L

A

A

A
|
A

I

L

M

M

S
|
S

T
x
S

V

L

D

A

S

S

L

I

L

F

L

N

I

S

V

S

S

S

S

T

A

L

I

F

V

T

K

M

D

D

V

I

Y

Y

I

T

N

R

Y

-

V

L

N

R

P

P

S

R

A

A

T

G

N

D

E

R

K

E

I

L

binding (2R,3R,4S,5S,6R)-2-[3-[(4-cyclopropylphenyl)methyl]-4-fluoranyl-indol-1-yl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (≠ Y57), H60 (≠ S62), G63 (= G65), L64 (≠ G66), T67 (≠ L69), V75 (= V86), F78 (≠ Y89), E79 (vs. gap), V137 (≠ A143), V266 (≠ L247), S267 (≠ V248), W271 (≠ V252)
binding sodium ion: A53 (= A55), I56 (≠ G58), G57 (≠ S59), A369 (= A333), S372 (= S336), S373 (≠ T337)

Sites not aligning to the query:

7vsiA Structure of human sglt2-map17 complex bound with empagliflozin (see paper)
23% identity, 77% coverage: 2:367/478 of query aligns to 2:402/586 of 7vsiA

query
sites
7vsiA

N

N

I

P

T

A

V

D

T

I

I

L

V

V

L

I

F

A

L

A

F

Y

L

F

C

L

I

L

I

V

F

I

A

G

V

V

G

G

F

L

W

W

A

S

S

M

K

-

S

-

V

C

Q

R

T

T

S

N

E

R

S

G

F

T

L

V

Q

G

E

G

Y

Y

F

F

L

L

G

A

G

G

R

R

Q

S

L

M

G

V

G

W

F

W

I

P

L

V

A

G

M

A

T

S

M

L

T

F

A

A

T

S

Y
x
N

G

I

S

G

A

S

S

G

S
x
H

F

F

I

V

G
|
G

G
x
L

P

A

G

G

L

T

A

G

Y

A

N

A

Q

S

G

G

L

L

G

A

W

-

V

-

L

-

A

-

M

-

S

-

Q

-

V

-

V
|
V

T

A

G

G

Y
x
F

-
x
E

-

W

-

N

-

A

-

L

F

F

V

V

L

V

M

L

V

L

L

L

G

G

K

W

R

L

F

F

A

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I

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A

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R

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Y

A

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K

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L

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F

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F

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A

K

M

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A

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G

G

A

A

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V

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F

I

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Q

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Q

L

A

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L

G

G

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N

-

I

Y

Y

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A

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S

A

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L

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F

A

L

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L

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A

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A

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G

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G

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Y

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F

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K

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Y

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A

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S

-

L

-

T

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V

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S

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D

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-

A

V

V

N

G

N

N

I

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N

D

-

S

-

Y

M

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A

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N

T

G

G

D

D

L

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-

W

P

P

A

A

Y

L

V

L

S

L

S

G

F

L

W

T

I

I

L
x
V

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x
S

G

G

V

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G
x
Y

V

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C

G

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G

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