SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8y5iA Cryo-em structure of e.Coli spermidine transporter potd-potabc in translocation intermidiate state (see paper)
43% identity, 68% coverage: 1:239/351 of query aligns to 2:235/358 of 8y5iA

query
sites
8y5iA

M

L

I

V

E

Q

F

L

K

A

N

G

V

I

S

R

K

K

S

C

Y
x
F

K

-

D

D

G

G

A
x
K

K

E

A

V

V

I

K

P

H

Q

I

L

S

D

F

L

T

T

V

I

E

N

G

G

E

E

L

F

L

L

T

T

L

L

I

L

G

G

P

P

S

S

G
|
G

C

C

G

G

K
|
K

T
|
T

T

V

L

L

K

R

M

L

I

I

N

A

R

G

L

L

I

E

E

T

P

V

T

D

E

S

G

G

H

R

I

I

Y

M

I

L

R

D

N

N

R

E

D

D

I

I

S

T

K

H

Y

V

K

P

M

A

N

-

E

E

L

N

R

R

W

Y

N

-

I

V

G

N

Y

T

V

V

L

F

Q

Q

Q

S

I

Y

A

A

L

L

F

F

P

P

H

H

M

M

T

T

I

V

E

F

E

E

N

N

I

V

A

A

I

F

V

G

P

L

E

R

M

M

K

Q

S

K

W

T

S

P

K

A

S

A

K

E

I

I

R

T

A

P

R

R

I

V

D

M

E

E

L

A

M

L

N

R

M

M

V

V

G

Q

L

L

D

E

P

-

V

-

T

T

Y

F

R

A

K

Q

R
|
R

K

K

P

P

S

H

E
x
Q

L

L

S
|
S

G

G

Q

Q

R

R

V

V

G

A

V

I

I

A

R

R

A

A

L

V

A

V

A

N

D

K

P

P

D

R

I

L

L

L

M

L

D

D

E

Q

P

S

F

L

S

S

A

A

L

L

D

D

P

Y

I

K

S

L

R

R

E

K

Q

Q

L

M

Q

Q

I

N

D

E

I

L

Q

K

H

A

L

L

Q

Q

K

R

E

K

I

L

K

G

K

I

T

T

I

F

V

V

F

F

V

V

T

T

H

H

D

D

I

Q

E

E

A

A

L

L

A

T

L

M

G

S

D

D

R

R

V

I

C

V

L

V

M

M

N

R

E

D

G

G

E

R

I

I

V

E

Q

Q

I

D

S

G

T

T

P

P

Q

R

E

E

I

I

I

Y

L

E

Q

E

P

P

E

K

N

N

A

L

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

E

binding adenosine-5'-triphosphate: F12 (≠ Y11), K15 (≠ A15), G38 (= G38), K41 (= K41), T42 (= T42), T43 (= T43), R128 (= R132), Q132 (≠ E136), S134 (= S138)

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
41% identity, 68% coverage: 2:238/351 of query aligns to 7:230/353 of 1vciA

query
sites
1vciA

I

V

E

K

F

L

K

E

N

N

V

L

S

T

K

K

S

R

Y
x
F

K

G

D

N

G

-

A
x
F

K

T

A

A

V

V

K

N

H

K

I

L

S

N

F

L

T

T

V

I

E

K

E

D

G

G

E

E

L

F

L

L

T

V

L

L

I

L

G

G

P

P

S
|
S

G
|
G

C

C

G
|
G

K
|
K

T
|
T

T

T

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

E

P

P

T

T

E

E

G

G

H

R

I

I

Y

Y

I

F

R

G

N

D

R

R

D

D

I

V

S

T

K

Y

Y

L

K

P

M

P

N

K

E

D

L

-

R

-

W

R

N

N

I

I

G

S

Y

M

V

V

L

F

Q

Q

Q

-

I

-

A

-

L

-

F

-

P

-

H

H

M

M

T

T

I

V

E

Y

E

E

N

N

I

I

A

A

I

F

V

-

P

-

E

P

M

L

K

K

S

K

W

F

S

P

K

K

S

D

K

E

I

I

R

D

A

K

R

R

I

V

D

R

E

W

L

A

M

A

N

E

M

L

V

L

G

Q

L

I

D

E

P

E

V

L

T

L

Y

-

R

-

K

N

R

R

K

Y

P

P

S

A

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

V

I

A

R

R

A

A

L

I

A

V

D

E

P

P

D

D

I

V

I

L

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

A

L

M

Q

R

I

A

D

E

I

I

Q

K

H

K

L

L

Q

Q

K

Q

E

K

I

L

K

K

K

V

T

T

I

T

V

I

F

Y

V

V

T

T

H

H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

M

G

G

D

D

R

R

V

I

C

A

L

V

M

M

N

N

E

R

G

G

E

Q

I

L

V

L

Q

Q

I

I

S

G

T

S

P

P

Q

T

E

E

I

V

I

Y

L

L

Q

R

P

P

E

N

N

S

A

V

F

F

V

V

K

A

N

T

F

F

V

I

G

G

binding adenosine-5'-triphosphate: F16 (≠ Y11), F19 (≠ A15), S41 (= S37), G42 (= G38), G44 (= G40), K45 (= K41), T46 (= T42), T47 (= T43)

8hprD Lpqy-sugabc in state 4 (see paper)
43% identity, 68% coverage: 2:238/351 of query aligns to 3:235/362 of 8hprD

query
sites
8hprD

I

I

E

V

F

L

K

D

N

R

V

V

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K

K

S

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Y
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Y

K

P

D

D

G

V

A

R

K

A

A

A

V

V

K

K

H

E

I

F

S

S

F

M

T

T

V

I

E

A

E

D

G

G

E

E

L

F

L

I

T

I

L

L

I

V

G

G

P

P

S
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S

G

G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

I

E

E

E

P

I

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G

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Y

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I

I

R

G

N

G

R

E

D

R

I

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S

N

K

E

Y

K

K

A

M

P

N

K

E

D

L

-

R

-

W

R

N

D

I

I

G

A

Y

M

V

V

L

F

Q
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Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

A

A

I

F

V

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L

E

T

M

L

K

A

S

K

W

V

S

P

K

K

S

A

K

E

I

I

R

A

A

A

R

K

I

V

D

E

E

E

L

T

M

A

N

K

M

I

V

L

G

D

L

L

D

S

P

E

V

L

T

L

Y

-

R

-

K

D

R
|
R

K

K

P

P

S

G

E
x
Q

L

L

S
|
S

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

S

P

P

D

K

I

A

I

F

L

L

M

M

D

D

E
x
Q

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

A

D

E

I

I

Q

S

H

R

L

L

Q

Q

K

D

E

R

I

L

K

G

K

T

T

T

I

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

T

E

E

A

A

L

M

A

T

L

L

G

G

D

D

R

R

V

V

C

V

L

V

M

M

N

L

E

A

G

G

E

E

I

V

V

Q

Q

Q

I

I

S

G

T

T

P

P

Q

D

E

E

I

L

I

Y

L

S

Q

S

P

P

E

A

N

N

A

L

F

F

V

V

K

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y11), S38 (= S37), C40 (= C39), G41 (= G40), K42 (= K41), S43 (≠ T42), T44 (= T43), Q82 (= Q83), R129 (= R132), Q133 (≠ E136), S135 (= S138), G136 (= G139), G137 (= G140), Q159 (≠ E162), H192 (= H195)
binding magnesium ion: S43 (≠ T42), Q82 (= Q83)

8hprC Lpqy-sugabc in state 4 (see paper)
43% identity, 68% coverage: 2:238/351 of query aligns to 3:235/363 of 8hprC

query
sites
8hprC

I

I

E

V

F

L

K

D

N

R

V

V

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K

K

S

S

Y
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Y

K

P

D

D

G

V

A

R

K

A

A

A

V

V

K

K

H

E

I

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S

S

F

M

T

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I

E

A

E

D

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G

E

E

L

F

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T

I

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L

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V

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G

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P

S
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S

G
|
G

C

C

G
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G

K
|
K

T
x
S

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

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E

E

E

P

I

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G

G

H

E

I

L

Y

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I

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G

N

G

R

E

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S

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K

E

Y

K

K

A

M

P

N

K

E

D

L

-

R

-

W

R

N

D

I

I

G

A

Y

M

V

V

L

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Q
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Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

A

A

I

F

V

P

P

L

E

T

M

L

K

A

S

K

W

V

S

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K

K

S

A

K

E

I

I

R

A

A

A

R

K

I

V

D

E

E

E

L

T

M

A

N

K

M

I

V

L

G

D

L

L

D

S

P

E

V

L

T

L

Y

-

R

-

K

D

R

R

K

K

P

P

S

G

E
x
Q

L

L

S

S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

S

P

P

D

K

I

A

I

F

L

L

M

M

D

D

E

Q

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

A

D

E

I

I

Q

S

H

R

L

L

Q

Q

K

D

E

R

I

L

K

G

K

T

T

T

I

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

T

E

E

A

A

L

M

A

T

L

L

G

G

D

D

R

R

V

V

C

V

L

V

M

M

N

L

E

A

G

G

E

E

I

V

V

Q

Q

Q

I

I

S

G

T

T

P

P

Q

D

E

E

I

L

I

Y

L

S

Q

S

P

P

E

A

N

N

A

L

F

F

V

V

K

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y11), S38 (= S37), G39 (= G38), G41 (= G40), K42 (= K41), S43 (≠ T42), Q82 (= Q83), Q133 (≠ E136), G136 (= G139), G137 (= G140), Q138 (= Q141), H192 (= H195)
binding magnesium ion: S43 (≠ T42), Q82 (= Q83)

P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 68% coverage: 2:238/351 of query aligns to 4:236/393 of P9WQI3

query
sites
P9WQI3

I

I

E

V

F

L

K

D

N

H

V

V

S

N

K

K

S

S

Y

Y

K

P

D

D

G

G

A

H

K

T

A

A

V

V

K

R

H

D

I

L

S

N

F

L

T

T

V

I

E

A

E

D

G

G

E

E

L

F

L

L

T

I

L

L

I

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

I

E

E

D

P

I

T

S

E

S

G

G

H

E

I

L

Y

R

I

I

R

A

N

G

R

E

D

R

I

V

S

N

K

E

Y

K

K

A

M

P

N

K

E

D

L

-

R

-

W

R

N

D

I

I

G

A

Y

M

V

V

L

F

Q

Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

A

A

I

F

V

P

P

L

E

T

M

L

K

A

S

K

W

M

S

R

K

K

S

A

K

D

I

I

R

A

A

Q

R

K

I

V

D

S

E

E

L

T

M

A

N

K

M

I

V

-

G

-

L

L

D

D

P

L

V

T

T

N

Y

L

R

L

K

D

R

R

K

K

P

P

S

S

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

H

P

P

D

K

I

A

I

F

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

G

D

E

I

I

Q

A

H

Q

L

L

Q

Q

K

R

E

R

I

L

K

G

K

T

T

T

I

T

V

V

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

T

E

E

A

A

L

M

A

T

L

L

G

G

D

D

R

R

V

V

C

V

L

V

M

M

N

Y

E

G

G

G

E

I

I

A

V

Q

Q

Q

I

I

S

G

T

T

P

P

Q

E

E

E

I

L

I

Y

L

E

Q

R

P

P

E

A

N

N

A

L

F

F

V

V

K

A

N

G

F

F

V

I

G

G

H193 (= H195) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.

1g291 Malk (see paper)
40% identity, 67% coverage: 17:252/351 of query aligns to 18:252/372 of 1g291

query
sites
1g291

A

A

V

V

K

R

H

E

I

M

S

S

F

L

T

E

V

V

E

K

E

D

G

G

E

E

L

F

L

M

T

I

L

L

I

L

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
|
T

T

T

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

E

P

P

T

S

E

R

G

G

H

Q

I

I

Y

Y

I

I

R

G

N

D

R

K

D

L

I

V

S

A

K
x
D

Y

P

K
x
E

M
x
K

N

G

E

I

L

F

-

V

-

P

-
x
K

R
x
D

W

R

N

D

I

I

G

A

Y

M

V

V

L

F

Q

Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

E

Y

E

D

N

N

I

I

A

A

I

F

V

P

P

L

E

K

M

L

K

R

S

K

W

V

S

P

K

R

S

Q

K

E

I

I

R

D

A

Q

R

R

I

V

D

R

E

E

L

V

M

A

N

E

M

L

V

L

G

G

L

L

D

T

P

E

V

L

T

L

Y

-

R

-

K

N

R

R

K

K

P

P

S

R

E

E

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

L

I

G

R

R

A

A

L

I

A

V

A

R

D

K

P

P

D

Q

I

V

I

F

L

L

M

M

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

R

L

M

Q

R

I

A

D

E

I

L

Q

K

H

K

L

L

Q

Q

K

R

E

Q

I

L

K

G

K

V

T

T

I

T

V

I

F

Y

V

V

T

T

H

H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

M

G

G

D

D

R

R

V

I

C

A

L

V

M

M

N

N

E

R

G

G

E

V

I

L

V

Q

Q

Q

I

V

S

G

T

S

P

P

Q

D

E

E

I

V

I

Y

L

D

Q

K

P

P

E

A

N

N

A

T

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

-

R

-

K

-

S

S

P

P

W

P

Q

M

T

N

A

F

V

L

D

D

V

A

M

I

V

V

binding magnesium ion: D69 (≠ K68), E71 (≠ K70), K72 (≠ M71), K79 (vs. gap), D80 (≠ R75)
binding pyrophosphate 2-: S38 (= S37), G39 (= G38), C40 (= C39), G41 (= G40), K42 (= K41), T43 (= T42), T44 (= T43)

Sites not aligning to the query:

binding magnesium ion: 292, 293, 359

8hplC Lpqy-sugabc in state 1 (see paper)
43% identity, 68% coverage: 2:238/351 of query aligns to 3:233/384 of 8hplC

query
sites
8hplC

I

I

E

V

F

L

K

D

N

R

V

V

S

T

K

K

S

S

Y
|
Y

K

P

D

R

G

A

A

A

K

-

A

-

V

V

K

K

H

E

I

F

S

S

F

M

T

T

V

I

E

A

E

D

G

G

E

E

L

F

L

I

T

I

L

L

I

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

T

L

L

K

N

M

M

I

I

N

A

R

G

L

L

I

E

E

E

P

I

T

T

E

S

G

G

H

E

I

L

Y

R

I

I

R

G

N

G

R

E

D

R

I

V

S

N

K

E

Y

K

K

A

M

P

N

K

E

D

L

-

R

-

W

R

N

D

I

I

G

A

Y

M

V

V

L

F

Q

Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

M

T

T

I

V

E

R

E

Q

N

N

I

I

A

A

I

F

V

P

P

L

E

T

M

L

K

A

S

K

W

V

S

P

K

K

S

A

K

E

I

I

R

A

A

A

R

K

I

V

D

E

E

E

L

T

M

A

N

K

M

I

V

-

G

-

L

L

D

D

P

L

V

S

T

E

Y

L

R

L

K

D

R

R

K

K

P

P

S

G

E

Q

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

M

I

G

R

R

A

A

L

I

A

V

A

R

D

S

P

P

D

K

I

A

I

F

L

L

M

M

D

D

E

Q

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

K

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

A

D

E

I

I

Q

S

H

R

L

L

Q

Q

K

D

E

R

I

L

K

G

K

T

T

T

I

T

V

V

F

Y

V

V

T

T

H

H

D

D

I

Q

E

T

E

E

A

A

L

M

A

T

L

L

G

G

D

D

R

R

V

V

C

V

L

V

M

M

N

L

E

A

G

G

E

E

I

V

V

Q

Q

Q

I

I

S

G

T

T

P

P

Q

D

E

E

I

L

I

Y

L

S

Q

S

P

P

E

A

N

N

A

L

F

F

V

V

K

A

N

G

F

F

V

I

G

G

binding adenosine-5'-triphosphate: Y12 (= Y11), G37 (= G38), C38 (= C39), G39 (= G40), K40 (= K41), S41 (≠ T42), T42 (= T43)

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 4:238/369 of P19566

query
sites
P19566

I

V

E

Q

F

L

K

R

N

N

V

V

S

T

K

K

S

A

Y

W

K

G

D

D

G

-

A

V

K

V

A

V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

D

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

-

N

-

R

-

D

-

I

-

S

G

K

E

Y

T

K

R

M

M

N

N

E

D

L

I

-

P

-

A

R

E

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q

Q

Q

S

I

Y

A

A

L
|
L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S

K

K

K

S

E

K

V

I

M

R

N

A

Q

R

R

I

V

D

N

E

Q

L

V

M

A

N

E

M

V

V

L

G

Q

L

L

D

A

P

H

V

L

T

L

Y

-

R

-

K

E

R

R

K

K

P

P

S

K

E

A

L

L

S

S

G

G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

R

I

V

I

F

L

L

M

L

D

D

E

E

P
|
P

F

L

S

S

A

N

L

L

D
|
D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H

H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A

R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

L86 (= L87) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P163) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D168) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 3:237/371 of 3puyA

query
sites
3puyA

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y
x
W

K

G

D

E

G

-

A

V

K

V

A

V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

G

N

E

R

K

D

R

I

M

S

N

K

D

Y

T

K

P

M

P

N

A

E

E

L

-

R

-

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q
|
Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S

K

K

K

S

E

K

V

I

I

R

N

A

Q

R

R

I

V

D

N

E

Q

L

V

M

A

N

E

M

V

V

L

G

Q

L

L

D

A

P

H

V

L

T

L

Y

-

R

-

K

D

R
|
R

K

K

P

P

S

K

E
x
A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

S

I

V

I

F

L

L

M

L

D

D

E
|
E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A

R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ Y11), S37 (= S37), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), Q81 (= Q83), R128 (= R132), A132 (≠ E136), S134 (= S138), G136 (= G140), Q137 (= Q141), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q83)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 3:237/371 of 3puxA

query
sites
3puxA

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y
x
W

K

G

D

E

G

-

A

V

K

V

A

V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

G

N

E

R

K

D

R

I

M

S

N

K

D

Y

T

K

P

M

P

N

A

E

E

L

-

R

-

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q
|
Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S

K

K

K

S

E

K

V

I

I

R

N

A

Q

R

R

I

V

D

N

E

Q

L

V

M

A

N

E

M

V

V

L

G

Q

L

L

D

A

P

H

V

L

T

L

Y

-

R

-

K

D

R
|
R

K

K

P

P

S

K

E

A

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

S

I

V

I

F

L

L

M

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A

R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

binding adenosine-5'-diphosphate: W12 (≠ Y11), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), S134 (= S138), Q137 (= Q141)
binding beryllium trifluoride ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q83), S134 (= S138), G136 (= G140), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q83)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 3:237/371 of 3puwA

query
sites
3puwA

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y
x
W

K

G

D

E

G

-

A

V

K

V

A
x
V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

G

N

E

R

K

D

R

I

M

S

N

K

D

Y

T

K

P

M

P

N

A

E

E

L

-

R

-

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q
|
Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S

K

K

K

S

E

K

V

I

I

R

N

A

Q

R

R

I

V

D

N

E

Q

L

V

M

A

N

E

M

V

V

L

G

Q

L

L

D

A

P

H

V

L

T

L

Y

-

R

-

K

D

R
|
R

K

K

P

P

S

K

E
x
A

L

L

S
|
S

G
|
G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

S

I

V

I

F

L

L

M

L

D

D

E
|
E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H
|
H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A

R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ E136), S134 (= S138), Q137 (= Q141)
binding tetrafluoroaluminate ion: S37 (= S37), G38 (= G38), K41 (= K41), Q81 (= Q83), S134 (= S138), G135 (= G139), G136 (= G140), E158 (= E162), H191 (= H195)
binding magnesium ion: S42 (≠ T42), Q81 (= Q83)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 3:237/371 of 3puvA

query
sites
3puvA

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y
x
W

K

G

D

E

G

-

A

V

K

V

A
x
V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S

S

G
|
G

C
|
C

G
|
G

K
|
K

T
x
S

T
|
T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

G

N

E

R

K

D

R

I

M

S

N

K

D

Y

T

K

P

M

P

N

A

E

E

L

-

R

-

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q
|
Q

Q

S

I

Y

A

A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S

K

K

K

S

E

K

V

I

I

R

N

A

Q

R

R

I

V

D

N

E

Q

L

V

M

A

N

E

M

V

V

L

G

Q

L

L

D

A

P

H

V

L

T

L

Y

-

R

-

K

D

R
|
R

K

K

P

P

S

K

E
x
A

L

L

S
|
S

G

G

Q
|
Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

S

I

V

I

F

L

L

M

L

D

D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H

H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A

R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

binding adenosine-5'-diphosphate: W12 (≠ Y11), V17 (≠ A17), G38 (= G38), C39 (= C39), G40 (= G40), K41 (= K41), S42 (≠ T42), T43 (= T43), R128 (= R132), A132 (≠ E136), S134 (= S138), Q137 (= Q141)
binding magnesium ion: S42 (≠ T42), Q81 (= Q83)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
35% identity, 68% coverage: 2:241/351 of query aligns to 4:238/371 of P68187

query
sites
P68187

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y

W

K

G

D

E

G

-

A

V

K

V

A

V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S

S

G

G

C

C

G

G

K

K

T

S

T

T

T

L

L

L

K

R

M

M

I

I

N

A

R

G

L

L

I

E

E

T

P

I

T

T

E

S

G

G

H

D

I

L

Y

F

I

I

R

G

N

E

R

K

D

R

I

M

S

N

K

D

Y

T

K

P

M

P

N

A

E

E

L

-

R

-

W

R

N

G

I

V

G

G

Y

M

V

V

L

F

Q

Q

Q

S

I

Y

A
|
A

L

L

F

Y

P

P

H

H

M

L

T

S

I

V

E

A

E

E

N

N

I

M

A

S

I

F

V

G

P

L

E

K

M

L

K

A

S

G

W

A

S
x
K

K

K

S

E

K

V

I

I

R

N

A

Q

R

R

I
x
V

D

N

E

Q

L
x
V

M

A

N
x
E

M

V

V

L

G

Q

L

L

D
x
A

P

H

V

L

T

L

Y

-

R

-

K

D

R

R

K

K

P

P

S

K

E

A

L

L

S

S

G

G

G
|
G

Q

Q

Q

R

Q

Q

R

R

V

V

G

A

V

I

I

G

R

R

A

T

L

L

A

V

A

A

D

E

P

P

D

S

I

V

I

F

L

L

M

L

D
|
D

E

E

P

P

F

L

S

S

A

N

L

L

D

D

P

A

I

A

S

L

R

R

E

V

Q

Q

L

M

Q

R

I

I

D

E

I

I

Q

S

H

R

L

L

Q

H

K

K

E

R

I

L

K

G

K

R

T

T

I

M

V

I

F

Y

V

V

T

T

H

H

D

D

I

Q

E

V

E

E

A

A

L

M

A

T

L

L

G

A

D

D

R

K

V

I

C

V

L

V

M

L

N

D

E

A

G

G

E

R

I

V

V

A

Q

Q

I

V

S

G

T

K

P

P

Q

L

E

E

I

L

I

Y

L

H

Q

Y

P

P

E

A

N

D

A
x
R

F

F

V

V

K

A

N

G

F

F

V

I

G

G

E

S

R

P

K

K

A85 (= A86) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ S107) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ I115) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L118) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (≠ N120) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ D125) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G140) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D161) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
R228 (≠ A231) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.

Sites not aligning to the query:

241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
35% identity, 68% coverage: 2:241/351 of query aligns to 1:235/367 of 1q12A

query
sites
1q12A

I

V

E

Q

F

L

K

Q

N

N

V

V

S

T

K

K

S

A

Y
x
W

K

G

D

E

G

-

A

V

K

V

A

V

V

S

K

K

H

D

I

I

S

N

F

L

T

D

V

I

E

H

E

E

G

G

E

E

L

F

L

V

T

V

L

F

I

V

G

G

P

P

S
|
S

G
|
G

C
|
C

G
|
G