SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_036833956.1 NCBI__GCF_000775615.1:WP_036833956.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
57% identity, 49% coverage: 143:276/276 of query aligns to 4:137/138 of 3oa4A

query
sites
3oa4A
K
 
K
L
 
L
A
 
D
H
|
H
I
 
I
G
 
G
I
 
I
A
 
A
V
 
V
N
 
T
S
 
S
I
 
I
E
 
K
Q
 
D
A
 
V
L
 
L
P
 
P
F
 
F
Y
 
Y
T
 
V
N
 
G
A
 
S
L
 
L
G
 
K
L
 
L
R
 
K
L
 
L
T
 
L
G
 
G
V
 
M
E
 
E
E
 
D
V
 
L
A
 
P
S
 
S
E
 
Q
Q
 
G
V
 
V
K
 
K
V
x
I
A
 
A
F
 
F
V
 
L
E
 
E
I
 
I
G
 
G
E
 
E
T
 
S
Q
 
K
I
 
I
E
|
E
L
 
L
L
 
L
E
 
E
P
 
P
T
 
L
D
 
S
D
 
E
N
 
E
S
 
S
P
 
P
I
 
I
A
 
A
K
 
K
F
 
F
M
 
I
E
 
Q
K
 
K
R
 
R
G
 
G
E
 
E
G
 
G
I
 
I
H
 
H
H
|
H
I
 
I
A
 
A
V
 
I
E
 
G
V
 
V
T
 
K
D
 
S
I
 
I
E
 
E
E
 
E
R
 
R
L
 
I
R
 
Q
H
 
E
Y
 
V
K
 
K
E
 
E
K
 
N
G
 
G
I
 
V
S
 
Q
L
 
M
I
 
I
H
 
N
D
 
D
T
 
E
P
 
P
K
 
V
L
 
P
G
 
G
A
 
A
H
 
R
Q
 
G
S
 
A
Q
 
Q
V
 
V
A
 
A
F
 
F
L
 
L
H
 
H
P
 
P
K
 
R
A
 
S
T
 
A
N
 
R
G
 
G
V
 
V
L
 
L
M
 
Y
E
|
E
L
 
F
C
 
C
Q
 
E
L
 
K
E
 
K
K
 
E
E
 
Q
E
 
A
E
 
E

I3VE74 2-hydroxyisobutanoyl-CoA mutase small subunit; 2-hydroxyisobutyryl-CoA mutase small subunit; HCM small subunit; EC 5.4.99.64 from Aquincola tertiaricarbonis (see paper)
52% identity, 43% coverage: 1:118/276 of query aligns to 1:118/136 of I3VE74

query
sites
I3VE74
M
 
M
E
 
D
H
 
Q
K
 
T
Q
 
P
I
 
I
R
 
R
V
 
V
L
 
L
I
 
L
A
 
A
K
 
K
P
 
V
G
 
G
L
 
L
D
 
D
G
 
G
H
|
H
D
 
D
R
 
R
G
 
G
A
 
V
L
 
K
V
 
V
I
 
V
A
 
A
Q
 
R
A
 
A
L
 
L
R
 
R
D
 
D
H
 
A
G
 
G
M
 
M
E
 
D
V
 
V
I
 
I
Y
 
Y
T
 
S
G
 
G
L
 
L
R
 
H
Q
 
R
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
V
V
 
V
Q
 
N
A
 
T
A
 
A
I
 
I
Q
 
Q
E
 
E
D
 
D
V
 
V
D
 
D
V
 
V
I
 
L
G
 
G
L
 
V
S
 
S
S
 
L
L
 
L
S
 
S
G
 
G
A
 
V
H
 
Q
N
 
L
S
 
T
L
 
V
F
 
F
P
 
P
K
 
K
V
 
I
I
 
F
E
 
K
E
 
L
L
 
L
K
 
D
K
 
E
H
 
R
N
 
G
A
 
A
E
 
G
D
 
D
I
 
L
P
 
I
V
 
V
I
 
I
G
 
A
G
 
G
G
 
G
V
 
V
I
 
M
P
 
P
Y
 
D
E
 
E
D
 
D
I
 
A
P
 
A
P
 
A
L
 
I
E
 
R
K
 
K
Q
 
L
G
 
G
I
 
V
H
 
R
K
 
E
I
 
V
F
 
L
T
 
L
P
 
Q
G
 
D
T
 
T
P
 
P

4r3uC Crystal structure of 2-hydroxyisobutyryl-coa mutase (see paper)
53% identity, 41% coverage: 6:118/276 of query aligns to 4:116/131 of 4r3uC

query
sites
4r3uC
I
 
I
R
 
R
V
 
V
L
 
L
I
 
L
A
 
A
K
|
K
P
 
V
G
 
G
L
 
L
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
V
L
 
K
V
 
V
I
x
V
A
 
A
Q
 
R
A
 
A
L
 
L
R
 
R
D
 
D
H
 
A
G
 
G
M
 
M
E
 
D
V
 
V
I
 
I
Y
 
Y
T
 
S
G
 
G
L
 
L
R
 
H
Q
 
R
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
V
V
 
V
Q
 
N
A
 
T
A
 
A
I
 
I
Q
 
Q
E
 
E
D
 
D
V
 
V
D
 
D
V
 
V
I
 
L
G
|
G
L
 
V
S
|
S
S
 
L
L
|
L
S
 
S
G
 
G
A
 
V
H
 
Q
N
 
L
S
 
T
L
 
V
F
 
F
P
 
P
K
 
K
V
 
I
I
 
F
E
 
K
E
 
L
L
 
L
K
 
D
K
 
E
H
 
R
N
 
G
A
 
A
E
 
G
D
 
D
I
 
L
P
 
I
V
 
V
I
|
I
G
x
A
G
|
G
G
|
G
V
 
V
I
 
M
P
 
P
Y
 
D
E
 
E
D
 
D
I
 
A
P
 
A
P
 
A
L
 
I
E
 
R
K
 
K
Q
 
L
G
 
G
I
 
V
H
 
R
K
 
E
I
 
V
F
x
L
T
x
L
P
x
Q
G
 
D
T
|
T
P
 
P

5reqA Methylmalonyl-coa mutase, y89f mutant, substrate complex (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 591:720/725 of 5reqA

query
sites
5reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
|
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

4reqA Methylmalonyl-coa mutase substrate complex (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 592:721/726 of 4reqA

query
sites
4reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
|
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

1e1cA Methylmalonyl-coa mutase h244a mutant (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 593:722/727 of 1e1cA

query
sites
1e1cA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
x
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
|
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
x
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

7reqA Methylmalonyl-coa mutase, 2-carboxypropyl-coa inhibitor complex (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 591:720/725 of 7reqA

query
sites
7reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

6reqA Methylmalonyl-coa mutase, 3-carboxypropyl-coa inhibitor complex (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 593:722/727 of 6reqA

query
sites
6reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
x
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
|
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

3reqA Methylmalonyl-coa mutase, substrate-free state (poor quality structure) (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 591:720/725 of 3reqA

query
sites
3reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
|
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
|
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

2reqA Methylmalonyl-coa mutase, non-productive coa complex, in open conformation representing substrate-free state (see paper)
43% identity, 47% coverage: 2:131/276 of query aligns to 591:720/725 of 2reqA

query
sites
2reqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
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D
G
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G
H
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H
D
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D
R
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R
G
|
G
A
 
Q
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
|
G
L
 
V
S
|
S
S
 
S
L
|
L
S
x
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
x
Y
T
|
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

P11653 Methylmalonyl-CoA mutase large subunit; MCM-alpha; EC 5.4.99.2 from Propionibacterium freudenreichii subsp. shermanii (see 4 papers)
43% identity, 47% coverage: 2:131/276 of query aligns to 594:723/728 of P11653

query
sites
P11653
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
L
A
 
A
K
 
K
P
 
M
G
 
G
L
 
Q
D
 
D
G
|
G
H
|
H
D
|
D
R
|
R
G
 
G
A
 
Q
L
 
K
V
 
V
I
 
I
A
 
A
Q
 
T
A
 
A
L
 
Y
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
T
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
V
Q
 
E
E
 
A
D
 
D
V
 
V
D
 
H
V
 
V
I
 
V
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
 
G
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
K
E
 
E
L
 
L
K
 
D
K
 
K
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
I
I
 
T
G
 
V
G
 
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
E
E
 
Q
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
R
K
 
K
Q
 
D
G
 
G
I
 
A
H
 
V
K
 
E
I
 
I
F
 
Y
T
 
T
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
P
E
 
E
T
 
S
A
 
A
L
 
I
Y
 
S
I
 
L
K
 
V
R
 
K
I
 
K
L
 
L
Q
 
R

Sites not aligning to the query:

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
41% identity, 49% coverage: 135:270/276 of query aligns to 39:175/176 of Q96PE7

query
sites
Q96PE7
T
 
T
S
 
G
S
 
S
L
 
V
T
 
W
P
 
N
P
 
L
E
 
G
K
 
R
L
 
L
A
 
N
H
|
H
I
 
V
G
 
A
I
 
I
A
 
A
V
 
V
N
 
P
S
 
D
I
 
L
E
 
E
Q
 
K
A
 
A
L
 
A
P
 
A
F
 
F
Y
 
Y
T
 
K
N
 
N
A
 
I
L
 
L
G
 
G
L
 
A
R
 
Q
L
 
V
T
 
S
G
 
E
V
 
A
E
 
V
E
 
P
V
 
L
A
 
P
S
 
E
E
 
H
Q
 
G
V
 
V
K
 
S
V
 
V
A
 
V
F
 
F
V
 
V
E
 
N
I
 
L
G
 
G
E
 
N
T
 
T
Q
 
K
I
 
M
E
 
E
L
 
L
L
 
L
E
 
H
P
 
P
T
 
L
D
 
G
D
x
R
N
 
D
S
 
S
P
 
P
I
 
I
A
 
A
K
 
G
F
 
F
M
 
L
E
 
Q
K
 
K
-
 
N
R
 
K
G
 
A
E
 
G
G
 
G
I
 
M
H
 
H
H
|
H
I
 
I
A
 
C
V
 
I
E
 
E
V
 
V
T
 
D
D
 
N
I
 
I
E
 
N
E
 
A
R
 
A
L
 
V
R
 
M
H
 
D
Y
 
L
K
 
K
E
 
K
K
 
K
G
 
K
I
 
I
S
 
R
L
 
S
I
 
L
H
 
S
D
 
E
T
 
E
P
 
V
K
 
K
L
 
I
G
 
G
A
 
A
H
 
H
Q
 
G
S
 
K
Q
 
P
V
 
V
A
 
I
F
 
F
L
 
L
H
 
H
P
 
P
K
 
K
A
 
D
T
 
C
N
 
G
G
 
G
V
 
V
L
 
L
M
 
V
E
|
E
L
 
L
C
 
E
Q
 
Q

P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
40% identity, 49% coverage: 2:136/276 of query aligns to 611:749/750 of P22033

query
sites
P22033
E
|
E
H
x
G
K
x
R
Q
x
R
I
x
P
R
|
R
V
x
L
L
|
L
I
x
V
A
|
A
K
|
K
P
x
M
G
|
G
L
x
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
|
A
L
x
K
V
|
V
I
|
I
A
|
A
Q
x
T
A
x
G
L
x
F
R
x
A
D
|
D
H
x
L
G
|
G
M
x
F
E
x
D
V
|
V
I
x
D
Y
x
I
T
x
G
G
x
P
L
|
L
R
x
F
Q
|
Q
S
x
T
P
|
P
Q
x
R
Q
x
E
I
x
V
V
x
A
Q
|
Q
A
x
Q
A
|
A
I
x
V
Q
x
D
E
x
A
D
|
D
V
|
V
D
x
H
V
x
A
I
x
V
G
|
G
L
x
I
S
|
S
S
x
T
L
|
L
S
x
A
G
x
A
A
x
G
H
|
H
N
x
K
S
x
T
L
|
L
F
x
V
P
|
P
K
x
E
V
x
L
I
|
I
E
x
K
E
|
E
L
|
L
K
x
N
K
x
S
H
x
L
N
x
G
A
x
R
E
x
P
D
|
D
I
|
I
P
x
L
V
|
V
I
x
M
G
x
C
G
|
G
G
|
G
V
|
V
I
|
I
P
|
P
Y
x
P
E
x
Q
D
|
D
I
x
Y
P
x
E
P
x
F
L
|
L
E
x
F
K
x
E
Q
x
V
G
|
G
I
x
V
H
x
S
K
x
N
I
x
V
F
|
F
T
x
G
P
|
P
G
|
G
T
|
T
P
x
R
T
x
I
E
x
P
E
x
K
T
x
A
A
|
A
L
x
V
Y
x
Q
-
x
V
-
x
L
-
x
D
-
x
D
I
|
I
K
x
E
R
x
K
I
x
C
L
|
L
Q
x
E
P
x
K
E
x
K
Q
|
Q
T
x
Q
S
|
S

Sites not aligning to the query:

2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
40% identity, 49% coverage: 2:136/276 of query aligns to 576:714/714 of 2xiqA

query
sites
2xiqA
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
L
L
 
L
I
 
V
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
A
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
G
L
 
F
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
I
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
R
Q
 
E
I
 
V
V
 
A
Q
 
Q
A
 
Q
A
 
A
I
 
V
Q
 
D
E
 
A
D
 
D
V
 
V
D
 
H
V
 
A
I
 
V
G
|
G
L
 
V
S
|
S
S
 
T
L
|
L
S
 
A
G
x
A
A
 
G
H
 
H
N
 
K
S
 
T
L
 
L
F
 
V
P
 
P
K
 
E
V
 
L
I
 
I
E
 
K
E
 
E
L
 
L
K
 
N
K
 
S
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
V
I
 
M
G
 
C
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
P
E
 
Q
D
 
D
I
 
Y
P
 
E
P
 
F
L
 
L
E
 
F
K
 
E
Q
 
V
G
 
G
I
 
V
H
 
S
K
 
N
I
 
V
F
|
F
T
x
G
P
 
P
G
 
G
T
|
T
P
 
R
T
 
I
E
 
P
E
 
K
T
 
A
A
 
A
L
 
V
Y
 
Q
-
 
V
-
 
L
-
 
D
-
 
D
I
 
I
K
 
E
R
 
K
I
 
C
L
 
L
Q
 
E
P
 
K
E
 
K
Q
 
Q
T
 
Q
S
 
S

Sites not aligning to the query:

8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
41% identity, 45% coverage: 2:126/276 of query aligns to 575:699/708 of 8dyjB

query
sites
8dyjB
E
 
E
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
L
L
 
L
I
 
V
A
 
A
K
 
K
P
 
M
G
 
G
L
 
Q
D
 
D
G
|
G
H
|
H
D
|
D
R
|
R
G
|
G
A
 
A
L
 
K
V
 
V
I
|
I
A
 
A
Q
 
T
A
 
G
L
 
F
R
 
A
D
 
D
H
 
L
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
I
T
 
G
G
 
P
L
 
L
R
 
F
Q
 
Q
S
 
T
P
 
P
Q
 
R
Q
 
E
I
 
V
V
 
A
Q
 
Q
A
 
Q
A
 
A
I
 
V
Q
 
D
E
 
A
D
 
D
V
 
V
D
 
H
V
 
A
I
 
V
G
 
G
L
 
V
S
|
S
S
 
T
L
|
L
S
 
A
G
x
A
A
 
G
H
 
H
N
 
K
S
 
T
L
 
L
F
 
V
P
 
P
K
 
E
V
 
L
I
 
I
E
 
K
E
 
E
L
 
L
K
 
N
K
 
S
H
 
L
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
L
V
 
V
I
 
M
G
 
C
G
|
G
G
|
G
V
|
V
I
 
I
P
 
P
Y
 
P
E
 
Q
D
 
D
I
 
Y
P
 
E
P
 
F
L
 
L
E
 
F
K
 
E
Q
 
V
G
 
G
I
 
V
H
 
S
K
 
N
I
 
V
F
|
F
T
x
G
P
 
P
G
 
G
T
|
T
P
 
R
T
 
I
E
 
P
E
 
K
T
 
A
A
 
A
L
 
V
Y
 
Q
I
 
V

Sites not aligning to the query:

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
42% identity, 46% coverage: 143:268/276 of query aligns to 11:135/138 of 6qh4C

query
sites
6qh4C
K
 
R
L
 
L
A
 
N
H
|
H
I
 
V
G
 
A
I
 
I
A
 
A
V
 
V
N
 
P
S
 
D
I
 
L
E
 
E
Q
 
K
A
 
A
L
 
A
P
 
A
F
 
F
Y
 
Y
T
 
K
N
 
N
A
 
I
L
 
L
G
 
G
L
 
A
R
 
Q
L
 
V
T
 
S
G
 
E
V
 
A
E
 
V
E
 
P
V
 
L
A
 
P
S
 
E
E
 
H
Q
 
G
V
 
V
K
 
S
V
|
V
A
 
V
F
 
F
V
 
V
E
 
N
I
 
L
G
 
G
E
 
N
T
 
T
Q
 
K
I
 
M
E
|
E
L
 
L
L
 
L
E
 
H
P
 
P
T
 
L
D
 
G
D
 
L
N
 
D
S
 
S
P
 
P
I
 
I
A
 
A
K
 
G
F
 
F
M
 
L
E
 
Q
K
 
K
-
 
N
R
 
K
G
 
A
E
 
G
G
 
G
I
 
M
H
 
H
H
|
H
I
 
I
A
 
C
V
 
I
E
 
E
V
 
V
T
 
D
D
 
N
I
 
I
E
 
N
E
 
A
R
 
A
L
 
V
R
 
M
H
 
D
Y
 
L
K
 
K
E
 
K
K
 
K
G
 
K
I
 
I
S
 
C
L
 
S
I
 
L
H
 
-
D
 
-
T
 
S
P
 
V
K
 
K
L
 
I
G
 
G
A
 
A
H
 
H
Q
 
G
S
 
K
Q
 
P
V
 
V
A
 
I
F
 
F
L
 
L
H
 
H
P
 
P
K
 
K
A
 
D
T
 
C
N
 
G
G
 
G
V
 
V
L
 
L
M
 
V
E
|
E
L
 
L

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
34% identity, 46% coverage: 143:268/276 of query aligns to 4:135/139 of 6wfhA

query
sites
6wfhA
K
 
R
L
 
I
A
 
D
H
|
H
I
 
I
G
 
G
I
 
I
A
 
A
V
 
C
N
 
H
S
 
D
I
 
L
E
 
D
Q
 
A
A
 
T
L
 
V
P
 
E
F
 
F
Y
 
Y
T
 
R
N
 
A
A
 
T
L
 
Y
G
 
G
L
 
F
R
 
E
L
 
V
T
 
F
G
 
H
V
 
T
E
 
E
E
 
V
V
 
N
A
 
E
S
 
E
E
x
Q
Q
 
G
V
 
V
K
 
R
V
x
E
A
 
A
F
 
M
V
 
L
E
 
K
I
 
I
G
 
N
E
 
D
T
 
T
Q
 
S
-
 
D
-
 
G
-
 
G
-
 
A
-
 
S
-
 
Y
I
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
T
 
T
D
 
R
D
 
E
N
 
D
S
 
S
P
x
A
I
 
V
A
 
G
K
|
K
F
x
W
M
 
L
E
 
A
K
 
K
R
 
N
G
 
G
E
 
E
G
 
G
I
 
V
H
|
H
H
|
H
I
 
I
A
 
A
V
 
F
E
 
G
V
 
T
T
 
A
D
 
D
I
 
V
E
 
D
E
 
A
R
 
D
L
 
A
R
 
A
H
 
D
Y
 
I
K
 
R
E
 
D
K
 
K
G
 
G
I
 
V
S
 
R
L
 
V
I
x
L
H
 
Y
D
 
D
T
 
E
P
 
P
K
 
R
L
 
R
G
|
G
A
x
S
H
 
M
Q
 
G
S
 
S
Q
 
R
V
 
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
A
 
D
T
 
C
N
 
H
G
 
G
V
 
V
L
|
L
M
 
T
E
|
E
L
 
L

6oxdA Structure of mycobacterium tuberculosis methylmalonyl-coa mutase with adenosyl cobalamin (see paper)
41% identity, 45% coverage: 2:124/276 of query aligns to 600:722/736 of 6oxdA

query
sites
6oxdA
E
 
D
H
 
G
K
 
R
Q
 
R
I
 
P
R
 
R
V
 
I
L
 
L
I
 
I
A
 
A
K
|
K
P
 
M
G
 
G
L
 
Q
D
|
D
G
|
G
H
|
H
D
|
D
R
|
R
G
 
G
A
 
Q
L
 
K
V
 
V
I
 
I
A
 
A
Q
 
T
A
 
A
L
 
F
R
 
A
D
 
D
H
 
I
G
 
G
M
 
F
E
 
D
V
 
V
I
 
D
Y
 
V
T
 
G
G
 
S
L
 
L
R
 
F
Q
 
S
S
 
T
P
 
P
Q
 
E
Q
 
E
I
 
V
V
 
A
Q
 
R
A
 
Q
A
 
A
I
 
A
Q
 
D
E
 
N
D
 
D
V
 
V
D
 
H
V
 
V
I
 
I
G
 
G
L
 
V
S
|
S
S
 
S
L
|
L
S
 
A
G
x
A
A
 
G
H
 
H
N
 
L
S
 
T
L
 
L
F
 
V
P
 
P
K
 
A
V
 
L
I
 
R
E
 
D
E
 
A
L
 
L
K
 
A
K
 
Q
H
 
V
N
 
G
A
 
R
E
 
P
D
 
D
I
 
I
P
 
M
V
 
I
I
 
V
G
 
V
G
|
G
G
|
G
V
 
V
I
 
I
P
 
P
Y
 
P
E
 
G
D
 
D
I
 
F
P
 
D
P
 
E
L
 
L
E
 
Y
K
 
A
Q
 
A
G
 
G
I
 
A
H
 
T
K
 
A
I
 
I
F
|
F
T
x
P
P
 
P
G
 
G
T
|
T
P
 
V
T
 
I
E
 
A
E
 
D
T
 
A
A
 
A
L
 
I

Sites not aligning to the query:

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
34% identity, 46% coverage: 143:268/276 of query aligns to 4:135/144 of 6xbqA

query
sites
6xbqA
K
 
R
L
 
I
A
 
D
H
|
H
I
 
I
G
 
G
I
 
I
A
 
A
V
 
C
N
 
H
S
 
D
I
 
L
E
 
D
Q
 
A
A
 
T
L
 
V
P
 
E
F
 
F
Y
 
Y
T
 
R
N
 
A
A
 
T
L
 
Y
G
 
G
L
 
F
R
 
E
L
 
V
T
 
F
G
 
H
V
 
T
E
 
E
E
 
V
V
 
N
A
 
E
S
 
E
E
x
Q
Q
 
G
V
 
V
K
 
R
V
x
E
A
 
A
F
 
M
V
 
L
E
 
K
I
 
I
G
 
N
E
 
D
T
 
T
Q
 
S
-
 
D
-
 
G
-
 
G
-
 
A
-
 
S
-
 
Y
I
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
T
 
T
D
 
R
D
 
E
N
 
D
S
 
S
P
x
A
I
 
V
A
 
G
K
|
K
F
x
W
M
 
L
E
 
A
K
 
K
R
 
N
G
 
G
E
 
E
G
 
G
I
 
V
H
|
H
H
|
H
I
 
I
A
 
A
V
 
F
E
 
G
V
 
T
T
 
A
D
 
D
I
 
V
E
 
D
E
 
A
R
 
D
L
 
A
R
 
A
H
 
D
Y
 
I
K
 
R
E
 
D
K
 
K
G
 
G
I
 
V
S
 
R
L
 
V
I
x
L
H
 
Y
D
 
D
T
 
E
P
 
P
K
 
R
L
 
R
G
 
G
A
 
S
H
 
M
Q
 
G
S
 
S
Q
 
R
V
 
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
|
K
A
 
D
T
 
C
N
 
H
G
 
G
V
 
V
L
|
L
M
 
T
E
|
E
L
 
L

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
34% identity, 46% coverage: 143:268/276 of query aligns to 4:135/144 of 6wfiA

query
sites
6wfiA
K
 
R
L
 
I
A
 
D
H
|
H
I
 
I
G
 
G
I
 
I
A
 
A
V
 
C
N
 
H
S
 
D
I
 
L
E
 
D
Q
 
A
A
 
T
L
 
V
P
 
E
F
 
F
Y
 
Y
T
 
R
N
 
A
A
 
T
L
 
Y
G
 
G
L
 
F
R
 
E
L
 
V
T
 
F
G
 
H
V
 
T
E
 
E
E
 
V
V
 
N
A
 
E
S
 
E
E
x
Q
Q
 
G
V
 
V
K
 
R
V
x
E
A
 
A
F
 
M
V
 
L
E
 
K
I
 
I
G
 
N
E
 
D
T
 
T
Q
 
S
-
 
D
-
 
G
-
 
G
-
 
A
-
 
S
-
 
Y
I
 
L
E
x
Q
L
 
L
L
 
L
E
 
E
P
 
P
T
 
T
D
 
R
D
 
E
N
 
D
S
 
S
P
x
A
I
 
V
A
 
G
K
|
K
F
x
W
M
 
L
E
 
A
K
 
K
R
 
N
G
 
G
E
 
E
G
 
G
I
 
V
H
|
H
H
|
H
I
 
I
A
 
A
V
 
F
E
 
G
V
 
T
T
 
A
D
 
D
I
 
V
E
 
D
E
 
A
R
 
D
L
 
A
R
 
A
H
 
D
Y
 
I
K
 
R
E
 
D
K
 
K
G
 
G
I
 
V
S
 
R
L
 
V
I
x
L
H
 
Y
D
 
D
T
 
E
P
 
P
K
 
R
L
 
R
G
|
G
A
x
S
H
 
M
Q
 
G
S
 
S
Q
 
R
V
x
I
A
 
T
F
|
F
L
 
L
H
 
H
P
|
P
K
 
K
A
 
D
T
 
C
N
 
H
G
 
G
V
 
V
L
|
L
M
 
T
E
|
E
L
 
L

Query Sequence

>WP_036833956.1 NCBI__GCF_000775615.1:WP_036833956.1
MEHKQIRVLIAKPGLDGHDRGALVIAQALRDHGMEVIYTGLRQSPQQIVQAAIQEDVDVI
GLSSLSGAHNSLFPKVIEELKKHNAEDIPVIGGGVIPYEDIPPLEKQGIHKIFTPGTPTE
ETALYIKRILQPEQTSSLTPPEKLAHIGIAVNSIEQALPFYTNALGLRLTGVEEVASEQV
KVAFVEIGETQIELLEPTDDNSPIAKFMEKRGEGIHHIAVEVTDIEERLRHYKEKGISLI
HDTPKLGAHQSQVAFLHPKATNGVLMELCQLEKEEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory