SitesBLAST

V59 (= V26) to L: in dbSNP:rs4008659
Y135 (≠ L102) Important for permeability to glycerol; mutation to A: Strongly decreased permeability to glycerol. Mildly decreased water permeability.
H165 (≠ I127) mutation to A: Decreased permeability to glycerol. Mildly decreased water permeability.
G264 (= G222) to V: affects water and glycerol transport; dbSNP:rs62542743

Sites not aligning to the query:

1:32 mutation Missing: Decreased interaction with PLIN1.
12 R → C: in dbSNP:rs139297434

8c9hA Aqp7_inhibitor (see paper)
36% identity, 83% coverage: 1:230/276 of query aligns to 9:247/253 of 8c9hA

query
sites
8c9hA

M

V

T

R

E

E

F

F

L

L

A

A

E

E

L

F

I

M

G

S

T

T

M

Y

I

V

L

M

I

M

I

V

F

F

G

G

G

L

G

G

V

S

V

V

G

A

G

H

V

M

V

V

L

L

K

N

K

K

S

K

K

Y

A

G

E

S

G

Y

T

L

G

G

W

-

V

-

L

-

I

V

T

N

L

L

G

G

W
x
F

G

G

L

F

A

G

V

V

T

T

M

M

G

G

V

V

Y

H

A

V

V

A

G

G

N

R

V

I

S

S

G

G

A
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A

H
|
H

I

M

N

N

P

A

A

A

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V

T

T

I

F

G

A

F

N

A

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M

A

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G

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W

S

R

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K

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P

T

V

F

Y

L

V

A

L

G

G

Q

Q

F

F

I

L

G

G

A

S

F

F

L

L

G

A

V

A

I

T

V

I

-

Y

-

S

-

L

F

F

L

Y

N

T

Y

A

L

I

P

L

H

H

W

F

N

S

A

G

T

G

E

Q

-

L

-

M

-

V

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T

D

G

K

P

G

V

A

A

K

T

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A

A

G

V

I

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F

S

A

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D

Y

-

L

P

P

A

D

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H

N

M

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P

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R

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F

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E

E

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M

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L

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x
Q

M

L

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C

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L
x
F

F

A

I

I

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A

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Q

E

E

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G

A

L

L

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P

-

G

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x
I

G

G

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I

L

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I

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V

A

I

I

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G

M

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S

L

L

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G

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M

A

N

T

T

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G

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Y

A

A

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I

N

N

P

P

A

S

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R

D

D

I

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G

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R

I

I

A

F

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F

L

-

P

-

I

I

P

A

G

G

K

W

G

G

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K

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Q

-

V

-

F

-

S

-

N

G

G

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E

N

N

W

W

G

-

Y

-

A

W

W

W

V

V

P

P

L

V

F

V

G

A

P

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L

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L

G

G

G

A

V

Y

Y

L

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G

A

G

L

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F

I

Y

Y

binding ethyl 4-[(4-pyrazol-1-ylphenyl)methylcarbamoylamino]benzoate: F49 (≠ W44), A66 (= A61), H67 (= H62), L157 (= L144), Q158 (≠ V145), F162 (≠ L149), I181 (≠ V165)

6n1gA Crystal structure of aquaglyceroporin aqp7 (see paper)
36% identity, 83% coverage: 1:230/276 of query aligns to 3:241/249 of 6n1gA

query
sites
6n1gA

M

V

T

R

E

E

F

F

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L

A

A

E

E

L

F

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M

G

S

T

T

M

Y

I

V

L

M

I

M

I

V

F

F

G

G

G

L

G

G

V

S

V

V

G

A

G

H

V

M

V

V

L

L

K

N

K

K

S

K

K

Y

A

G

E

S

G

Y

T

L

G

G

W

-

V

-

L

-

I

V

T

N

L

L

G

G

W

F

G

G

L

F

A

G

V

V

T

T

M

M

G

G

V

V

Y

H

A

V

V

A

G

G

N

R

V

I

S

S

G

G

A

A

H

H

I

M

N

N

P

A

A

A

V

V

T

T

I

F

G

A

F

N

A

C

M

A

I
x
L

G

G

E
x
R

F

V

P

P

W

W

S

R

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K

V

F

P

P

T

V

F

Y

L

V

A

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G

G

Q

Q

F

F

I

L

G

G

A

S

F

F

L

L

G

A

V

A

I

T

V

I

-

Y

-

S

-

L

F

F

L

Y

N

T

Y

A

L

I

P

L

H

H

W

F

N

S

A

G

T

G

E

Q

-

L

-

M

-

V

-

T

D

G

K

P

G

V

A

A

K

T

L

A

A

G

V

I

F

F

S

A

T

T

D

Y

-

L

P

P

A

D

I

H

N

M

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P

L

L

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R

N

G

F

F

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E

E

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M

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C

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F

A

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A

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Q

E

E

F

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P

G

A

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L

-

P

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G

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T

N

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P

A

F

L

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V

I

G

G

L

I

L

L

I

V

V

V

A

I

I

I

G

G

M

V

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S

L

L

G

G

G

M

A

N

T

T

G

G

Y

Y

A

A

I

I

N

N

P

P

A

S

R

R

D

D

I

L

G

P

P

P

R

R

I

I

A

F

H

T

M

F

L

-

P

-

I

I

P

A

G

G

K

W

G

G

-

K

-

Q

-

V

-

F

-

S

-

N

G

G

S

E

N

N

W

W

G

-

Y

-

A

W

W

W

V

V

P

P

L

V

F

V

G

A

P

P

I

L

V

L

G

G

G

A

V

Y

Y

L

G

G

A

G

L

I

F

I

Y

Y

binding glycerol: L73 (≠ I74), R75 (≠ E76)

Q96PS8 Aquaporin-10; AQP-10; Aquaglyceroporin-10; Small intestine aquaporin from Homo sapiens (Human) (see 2 papers)
34% identity, 83% coverage: 3:231/276 of query aligns to 23:261/301 of Q96PS8

query
sites
Q96PS8

E

Q

F

C

L

L

A

A

E
|
E

L

F

I

L

G

G

T

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M

F

I

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L

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M

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A

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V

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A

G

Q

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A

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V

L

-

K

T

K

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K

E

A

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E

K

G

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N

G

F

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F

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M

I

F

T

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L

A

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G

W

-

G

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L

A

A

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V

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M

I

G

A

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I

Y

Y

A

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V
x
G

G

G

N

N

V

V

S
|
S

G

G

A

A

H
|
H

I

L

N

N

P

P

A

A

V
x
F

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S

I

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A

F

M

A

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M

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x
R

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I

A

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A

A

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A

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Y

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Y

L

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P

-

H

H

W

D

N

A

A

L

T

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E

N

D

Y

K

T

G

G

A

G

K
x
N

L

L

A

T

V

V

-

T

-

G

-

P

-

K

-

E

-

T

-

A

-

S

-

I

F

F

S

A

T

T

D

Y

P

P

A

A

I

P

N

Y

S

L

P

S

L

L

S

N

N

N

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G

F

F

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E

Q

I

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G

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T

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G

M

M

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L

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M

V

G

G

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A

I

I

G

L

A

D

N

R

E

R

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N

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K

-

G

F

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Q

A

G

G

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L

N

E

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F

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I

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G

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M

L

L

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I

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L

A

A

I

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G

G

M

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S

L

M

G

G

G

A

A

N

T

C

G

G

Y

I

A

P

I

L

N

N

P

P

A

A

R

R

D

D

I

L

G

G

P

P

R

R

I

L

A

F

H

T

M

Y

L

V

L

A

-

G

-

W

-

G

P

P

I

E

P

V

G

F

K

S

G

A

G

G

S

N

N

G

W

W

G

W

Y

-

A

-

W

W

V

V

P

P

L

V

F

V

G

A

P

P

I

L

V

V

G

G

G

A

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Y

V

G

G

A

T

L

A

F

T

Y

Y

K

Q

E27 (= E7) mutation to Q: Abolishes permeability to glycerol.
G73 (≠ V55) mutation to A: Increased permeability to glycerol at acidic pH.; mutation to F: Abolishes permeability to glycerol.
S77 (= S59) mutation S->A,D: Nearly abolishes permeability to glycerol.
H80 (= H62) mutation to A: Abolishes permeability to glycerol.
F85 (≠ V67) mutation to A: Nearly abolishes permeability to glycerol.
R94 (≠ E76) mutation to A: Abolishes permeability to glycerol.
N133 (≠ K117) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Abolishes N-glycosylation.

6f7hC Crystal structure of human aqp10 (see paper)
34% identity, 83% coverage: 3:231/276 of query aligns to 8:246/253 of 6f7hC

query
sites
6f7hC

E

Q

F

C

L

L

A

A

E

E

L

F

I

L

G

G

T

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M

F

I

V

L

L

I

M

I

L

F

L

G

T

G

Q

G

G

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A

V

V

G

A

G

Q

V

A

V

V

L

-

K

T

K

S

S

G

K

E

A

T

E

K

G

G

T

N

G

F

W

F

V

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L

M

I

F

T

L

L

A

G

G

W

-

G

S

L

L

A

A

V

V

T

T

M

I

G

A

V
x
I

Y

Y

A

V

V

G

G

G

N

N

V

V

S

S

G

G

A

A

H

H

I

L

N

N

P

P

A

A

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F

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G

A

F

M

A

C

M

I

I

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R

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Y

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A

L

G

V

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Q

F

L

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L

G

S

A

A

F

F

L

C

G

A

G

S

V

G

I

A

V

T

F

Y

L

V

N

L

Y

Y

L

-

P

-

H

H

W

D

N

A

A

L

T

Q

E

N

D

Y

K

T

G

G

A

G

K

N

L

L

A

T

V

V

-

T

-

G

-

P

-

K

-

E

-

T

-

A

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S

-

I

F

F

S

A

T

T

D

Y

P

P

A

A

I

P

N

Y

S

L

P

S

L

L

S

N

N

N

-

G

F

F

V

L

S

D

E

Q

I

V

I

L

G

G

T

T

F

G

M

M

L

L

V

I

M

V

G

G

L

L

F

A

I

I

G

L

A

D

N

R

E

R

-

N

-

K

-

G

F

V

T

P

Q

A

G

G

L

L

N

E

P

P

F

V

I

V

V

V

G

G

L

M

L

L

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I

V

L

A

A

I

L

G

G

M

L

S

S

L

M

G

G

G

A

A

N

T

C

G

G

Y

I

A

P

I

L

N
|
N

P

P

A

A

R

R

D

D

I

L

G

G

P

P

R

R

I

L

A

F

H

T

M

Y

L

V

L

A

-

G

-

W

-

G

P

P

I

E

P

V

G

F

K

S

G

A

G

G

S

N

N

G

W

W

G

W

Y

-

A

-

W

W

V

V

P

P

L

V

F

V

G

A

P

P

I

L

V

V

G

G

G

A

V

T

Y

V

G

G

A

T

L

A

F

T

Y

Y

K

Q

binding glycerol: I55 (≠ V52), N199 (= N185)

I1CR68 Aquaporin-1 from Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) (Mucormycosis agent) (Rhizopus arrhizus var. delemar) (see paper)
33% identity, 82% coverage: 3:229/276 of query aligns to 60:295/306 of I1CR68

query
sites
I1CR68

E

E

F

F

L

L

A

A

E

E

L

F

I

I

G

G

T

T

M

V

I

I

L

L

I

V

I

L

F

L

G

T

G

C

G

G

V

F

V

C

G

A

G

E

V

Q

V

T

L

L

K

H

K

I

S

E

K

-

A

-

E

E

G

S

T

K

G

S

W

W

V

L

L

T

I

S

T

S

L

F

G

G

W

S

G

G

L

L

A

S

V

V

T

L

M

I

G

G

V

I

Y

C

A

V

V

S

G

G

N

H

V

V

S

S

G

G

A

A

H

H

I

L

N

N

P

P

A

A

V

V

T

T

I

I

G

A

F

F

A

C

M

I

I

F

G

S

E

G

F

F

P

P

W

I

S

R

Q

K

V

V

P

P

T

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F

Y

L

I

A

T

G

A

Q

Q

F

L

I

L

G

G

A

A

F

F

L

A

G

G

G

A

V

A

I

L

V

L

F

Y

L

I

N

I

Y

I

L

E

P

P

-

A

-

I

-

V

H

Q

W

F

N

D

A

G

T

G

E

Q

D

R

-

Y

-

I

-

L

-

G

K

E

G

K

A

S

K

T

L

A

A

G

V

I

F

F

S

G

T

T

D

Y

P

P

A

P

I

L

N

Y

S

V

P

G

L

I

-

G

S

S

N

A

F

I

V

A

S

S

E

E

I

I

I

M

G

G

T

T

F

A

M

M

L

L

V

L

M

L

G

V

L

I

L

M

F

V

I

T

G

G

A

H

-

P

-

N

N

N

E

L

F

P

T

Y

Q

K

G

S

L

A

N

Q

P

G

F

A

I

M

V

I

G

A

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L

L

G

I

I

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T

A

T

I

I

G

S

M

L

S

C

L

I

G

G

G

Y

A

T

T

S

G

G

Y

F

A

S

I

L

N

N

P

P

A

A

R

R

D

D

I

F

G

G

P

P

R

R

I

-

A

-

H

-

M

L

L

F

L

T

P

A

I

I

P

A

G

G

K

-

G

-

S

-

N

-

W

W

G

G

-

F

-

D

-

V

-

F

-

K

-

V

-

Y

-
x
H

-

Y

Y

Y

A

A

W

L

V

V

P

P

L

M

F

F

G

A

P

P

I

I

V

L

G

G

V

L

Y

V

G

G

A

L

L

M

F

L

H275 (vs. gap) mutation to A: Affects pH sensing; when associated with A-85.

P0AER0 Glycerol uptake facilitator protein; Aquaglyceroporin; Glycerol facilitator from Escherichia coli (strain K12) (see 3 papers)
38% identity, 84% coverage: 3:233/276 of query aligns to 10:254/281 of P0AER0

query
sites
P0AER0

E

Q

F

C

L

I

A

A

E

E

L

F

I

L

G

G

T

T

M

G

I

L

L

L

I

I

I

F

F

F

G

G

G

V

G

G

V

C

V

V

G

A

V

-

L

L

K

K

K

V

S

A

K

G

A

A

E

S

G

F

T

G

G

Q

W

W

V

E

L

-

I

I

T

S

L

V

G

I

W

W

G

G

L

L

A

G

V

V

T

A

M

M

G

A

V

I

Y

Y

A

L

V

T

G

A

N

G

V

V

S

S

G

G

A

A

H
|
H

I
x
L

N
|
N

P

P

A

A

V

V

T

T

I

I

G

A

F

L

A

W

M

L

I

F

G

A

E

C

F

F

P

D

W

K

S

R

Q

K

V

V

P

I

T

P

F

F

L

I

A

V

G

S

Q

Q

F

V

I

A

G

G

A

A

F

F

L

C

G

A

V

A

I

L

V

V

-

Y

-

G

-

L

F

Y

L

Y

N

N

Y

L

L

F

P

F

H

D

W

F

N

E

A

Q

T

T

E

H

D

H

-

I

-

V

K

R

G

G

A

S

K

V

L

E

A

S

V

V

-

D

-

L

-

A

-

G

-

T

F

F

S

S

T

T

D
x
Y

P

P

A

-

I

-

N

-

S

N

P

P

L

H

S

I

N

N

F

F

V

V

S

Q

-

A

-

F

-

A

-

V

E

E

I

M

I

V

G

I

T

T

F

A

M

I

L

L

V

-

M

M

G

G

L

L

L

I

F

L

I

A

-

L

-

T

-

D

G

D

A

G

N

N

E

G

F

V

T

P

Q

R

G

G

-

P

L

L

N

A

P

P

F

L

I

L

V

I

G

G

L

L

I

I

V

A

A

V

I

I

G

G

M

A

S

S

L

M

G

G

G

P

A

L

T

T

G
|
G

Y
x
F

A
|
A

I

M

N
|
N

P

P

A

A

R
|
R

D

D

I

F

G

G

P

P

R

K

I

V

A

F

H

A

M

W

L

L

L

A

-

G

-

W

-

G

P

N

I

V

P

A

G

F

K

T

G

G

S

R

N

D

W

I

G

P

Y

Y

A

F

W

L

V

V

P
|
P

L
|
L

F

F

G

G

P

P

I

I

V

V

G

G

G

A

V

I

Y

V

G

G

A

A

L

F

F

A

Y

Y

K

R

A

K

I

L

HLN 66:68 (≠ HIN 62:64) binding glycerol
Y138 (≠ D124) binding glycerol
GFA 199:201 (≠ GYA 181:183) binding glycerol
N203 (= N185) binding glycerol
R206 (= R188) binding glycerol
PL 236:237 (= PL 215:216) mutation to FW: No detectable water or glycerol permeability.

1fx8A Crystal structure of the e. Coli glycerol facilitator (glpf) with substrate glycerol (see paper)
38% identity, 84% coverage: 3:233/276 of query aligns to 5:249/254 of 1fx8A

query
sites
1fx8A

E

Q

F

C

L

I

A

A

E

E

L

F

I

L

G

G

T

T

M

G

I

L

L

L

I

I

I

F

F

F

G

G

G

V

G

G

V

C

V

V

G

A

V

-

L

L

K

K

K

V

S

A

K

G

A

A

E

S

G

F

T

G

G

Q

W

W

V

E

L

-

I

I

T

S

L

V

G

I

W
|
W

G

G

L

L

A

G

V

V

T

A

M

M

G

A

V

I

Y

Y

A

L

V

T

G

A

N

G

V

V

S

S

G

G

A

A

H

H

I

L

N
|
N

P

P

A

A

V

V

T

T

I

I

G

A

F

L

A

W

M

L

I

F

G

A

E

C

F

F

P

D

W

K

S

R

Q

K

V

V

P

I

T

P

F

F

L

I

A

V

G

S

Q

Q

F

V

I

A

G

G

A

A

F

F

L

C

G

A

V

A

I

L

V

V

-

Y

-

G

-

L

F

Y

L

Y

N

N

Y

L

L

F

P

F

H

D

W

F

N

E

A

Q

T

T

E

H

D

H

-

I

-

V

K

R

G

G

A

S

K

V

L

E

A

S

V

V

-

D

-

L

-

A

-

G

-

T

F

F

S

S

T

T

D

Y

P

P

A

-

I

-

N

-

S

N

P

P

L

H

S

I

N

N

F

F

V

V

S

Q

-

A

-

F

-

A

-

V

E

E

I

M

I

V

G

I

T

T

F

A

M

I

L

L

V

-

M

M

G

G

L

L

L

I

F

L

I

A

-

L

-

T

-

D

G

D

A

G

N

N

E

G

F

V

T

P

Q

R

G

G

-

P

L

L

N

A

P

P

F

L

I

L

V

I

G

G

L

L

I

I

V

A

A

V

I

I

G

G

M

A

S

S

L

M

G

G

G

P

A

L

T

T

G
|
G

Y
x
F

A
|
A

I

M

N
|
N

P

P

A

A

R
|
R

D

D

I

F

G

G

P

P

R

K

I

V

A

F

H

A

M

W

L

L

L

A

-

G

-

W

-

G

P

N

I

V

P

A

G

F

K

T

G

G

S

R

N

D

W

I

G

P

Y

Y

A

F

W

L

V

V

P

P

L

L

F

F

G

G

P

P

I

I

V

V

G

G

G

A

V

I

Y

V

G

G

A

A

L

F

F

A

Y

Y

K

R

A

K

I

L

binding glycerol: W43 (= W44), N63 (= N64), G194 (= G181), F195 (≠ Y182), A196 (= A183), N198 (= N185), R201 (= R188)

3c02A X-ray structure of the aquaglyceroporin from plasmodium falciparum (see paper)
32% identity, 84% coverage: 1:233/276 of query aligns to 3:234/242 of 3c02A

query
sites
3c02A

M

V

T

R

E

E

F

F

L

I

A

G

E

E

L

F

I

L

G

G

T

T

M

F

I

V

L

L

I

M

I

F

F

L

G

G

G

E

G

G

V

A

V

T

G

A

G

N

V

F

V

-

L

-

K

-

K

H

S

T

K

T

A

G

E

L

G

S

T

G

G

D

W

W

V

Y

L

K

I

L

T

C

L

L

G

G

W
|
W

G

G

L

L

A

A

V

V

T

F

M

F

G

G

V

I

Y

L

A

V

V

S

G

A

N

K

V

L

S

S

G

G

A

A

H

H

I

L

N

N

P

L

A

A

V

V

T

S

I

I

G

G

F

L

A

S

M

S

I

I

G

N

E

K

F

F

P

D

W

L

S

K

Q

K

V

I

P

P

T

V

F

Y

L

F

A

F

G

A

Q

Q

F

L

I

L

G

G

A

A

F

F

L

V

G

G

G

T

V

S

I

T

V

V

F

Y

L

G

N

L

Y

Y

L

-

P

-

H

H

W

G

N

F

A

I

T

S

E

N

D

S

K

K

G

I

A

P

K

Q

L

F

A

A

-

W

V

E

F

T

S

S

T

R

D

N

P

P

A

S

I

I

N

-

S

S

P

L

L

T

S

G

N

A

F

F

V

F

S

N

E

E

I

L

I

I

G

L

T

T

F

G

M

I

L

L

V

L

M

L

G

V

L

I

L

L

F

V

I

V

G

V

A

D

N

E

E

N

F

I

T

C

Q

G

G

K

L

F

N

H

P

I

F

L

-

K

-

L

-

S

I

V

V

V

G

G

L

L

L

I

I

I

V

L

A

C

I

I

G

G

M

I

S

T

L

F

G

G

A

N

T

T

G
|
G

Y
x
F

A

A

I

L

N

N

P

P

A

S

R
|
R

D

D

I

L

G

G

P

S

R

R

I

F

A

L

H

S

M

L

L

I

L

A

P

Y

I

G

P

K

G

D

K

T

G

F

G

T

S

K

N

D

W

N

G

F

Y

Y

A

F

W

W

V

V

P

P

L

L

F

V

G

A

P

P

I

C

V

V

G

G

G

S

V

V

Y

V

G

F

A

C

L

Q

F

F

Y

Y

K

D

A

K

I

V

binding glycerol: W43 (= W44), G182 (= G181), F183 (≠ Y182), R189 (= R188)

2evuA Crystal structure of aquaporin aqpm at 2.3a resolution (see paper)
34% identity, 83% coverage: 3:231/276 of query aligns to 7:241/245 of 2evuA

query
sites
2evuA

E

R

F

C

L

I

A

A

E

E

L

F

I

I

G

G

T

T

M

F

I

I

L

L

I

V

I

F

F

F

G

G

G

A

G

G

V

S

V

A

G

A

G

V

V

T

V

L

L

M

K

I

K

A

S

S

K

G

A

G

E

T

G

S

-

P

-

N

-

P

-

F

-

N

-
x
I

-

G

-

I

-

G

-

L

-

G

-

L

T

G

G

D

W
|
W

V

V

L

A

I

I

T

G

L

L

G

A

W

F

G

G

L

F

A

A

V
x
I

T

A

M

A

G

S

V

I

Y

Y

A

A

V

L

G

G

N

N

V

I

S

S

G

G

A

C

H

H

I
|
I

N
|
N

P

P

A

A

V

V

T

T

I

I

G

G

F

L

A

W

M

S

I

V

G

K

E

K

F

F

P

P

W

G

S

R

Q

E

V

V

P

V

T

P

F

Y

L

I

A

I

G

A

Q

Q

F

L

I

L

G

G

A

A

F

A

L

F

G

G

G

S

V

F

I

I

V

-

F

F

L

L

N

Q

Y

C

L

A

P

G

H

I

W

G

N

A

A

A

T

T

E

V

D

G

K

G

G

L

A
x
G

K
x
A

L

T

A
|
A

V
x
P

F
|
F

S

-

T

-

D

-

P

P

A

G

I

I

N

-

S

S

P

Y

L

W

S

Q

N

A

F

M

V

L

S

A

E

E

I

V

G

G

T

T

F

F

M

L

L

L

V

M

M

I

G

T

L

I

L

M

F

G

I

I

G

A

A

V

N

D

E

E

F

R

T

A

-

P

Q

K

G

G

L

F

N

A

P

G

F

I

I

I

V

I

G

G

L

L

L

T

I

V

V

A

A

G

I

I

G

I

M

T

S

T

L

L

G

G

G

N

A

I

T

S

G

G

Y

S

A

S

I

L

N

N

P

P

A

A

R

R

D

T

I

F

G

G

P

P

R

Y

I

L

A

N

H

D

M

M

L

I

L

F

P

-

I

-

P

-

G

-

K

A

G

G

G

T

S

D

N

L

W

W

G

N

Y

Y

A

Y

W

S

V

I

P

Y

L

V

F

I

G

G

P

P

I

I

V

V

G

G

G

A

V

V

Y

L

G

A

A

A

L

L

F

T

Y

Y

K

Q

binding glycerol: I44 (vs. gap), W55 (= W37), I66 (≠ V48), I81 (= I63), N82 (= N64), G133 (≠ A116), A134 (≠ K117), A136 (= A119), P137 (≠ V120), F138 (= F121)

P37451 Propanediol uptake facilitator PduF from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
33% identity, 82% coverage: 6:231/276 of query aligns to 11:250/264 of P37451

query
sites
P37451

A

A

E

E

L

F

I

L

G

G

T

T

M

G

I

L

L

F

I

L

I

F

F

F

G

G

G

I

G

G

V

C

V

L

G

S

G

A

V

-

L

L

K

K

K

V

S

A

K

G

A

A

E

S

G

L

T

G

G

L

W

W

V

E

L

I

I

C

T

I

L

I

G

-

W

W

G

G

L

L

A

G

V

I

T

S

M

L

G

A

V

V

Y

Y

A

L

V

T

G

A

N

G

V

I

S

S

G

G

A

G

H

H

I

L

N
|
N

P
|
P

A
|
A

V

V

T

T

I

I

G

A

F

L

A

W

M

L

I

F

G

A

E

C

F

F

P

P

W

K

S

Q

Q

K

V

V

P

L

T

P

F

Y

L

I

A

I

G

A

Q

Q

F

F

I

A

G

G

A

A

F

F

L

G

G

G

G

A

V

L

I

L

V

A

F

Y

L

V

N

L

Y

Y

L

S

P

S

H

L

W

F

N

T

A

E

T

F

E

E

D

T

-

A

-

H

-

M

-

V

-

R

-

G

-

S

-

V

K

E

G

S

A

L

K

Q

L

L

A

A

-

S

V

I

F

F

S

S

T

T

D

Y

P

P

-

A

A

A

I

L

N

N

S

V

P

W

L

Q

S

A

N

A

F

L

V

V

S

E

E

V

I

V

I

I

G

T

T

S

F

I

M

L

-

M

-

G

L

M

V

I

M

M

G

A

L

L

L

T

F

D

I

D

G

G

A

-

N

N

E

G

F

I

T

P

Q

K

G

G

-

P

L

L

N

A

P

P

F

L

I

L

V

I

G

G

L

I

L

L

I

V

V

A

A

V

I

I

G

G

M

A

S

S

L

T

G

G

G

P

A

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

I

F

G

G

P

P

R

K

I

L

A

F

H

T

M

W

L

L

L

A

-

G

-

W

-

G

P

N

I

M

P

A

G

M

K

S

G

G

S

R

N

E

W

I

G

P

Y

Y

A

F

W

I

V

V

P

P

L

I

F

V

G

A

P

P

I

V

V

I

G

G

G

A

V

C

Y

A

G

G

A

A

L

A

F

I

Y

Y

K

R

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 185:187) NPA 2

B1VB61 Propanediol uptake facilitator PduF from Citrobacter freundii (see paper)
30% identity, 83% coverage: 3:231/276 of query aligns to 8:250/269 of B1VB61

query
sites
B1VB61

E

Q

F

C

L

I

A

A

E

E

L

F

I

L

G

G

T

T

M

G

I

L

L

F

I

L

I

F

F

F

G

G

G

I

G

G

V

C

V

L

G

S

G

A

V

-

L

L

K

K

K

V

S

A

K

G

A

A

E

S

G

L

T

G

G

L

W

W

V

E

L

I

I

C

T

I

L

I

G

-

W

W

G

G

L

L

A

G

V

I

T

S

M

L

G

A

V

V

Y

Y

A

L

V

T

G

A

N

G

V

I

S

S

G

G

A

A

H

H

I

L

N
|
N

P
|
P

A
|
A

V

I

T

T

I

I

G

A

F

L

A

W

M

L

I

F

G

A

E

C

F

F

P

P

W

G

S

R

Q

K

V

V

P

L

T

P

F

Y

L

T

A

V

G

A

Q

Q

F

V

I

A

G

G

A

A

F

F

L

G

G

G

G

A

V

L

I

L

V

A

F

Y

L

L

N

L

Y

Y

L

G

P

S

H

L

W

F

N

T

A

E

T

Y

E

E

D

S

K

A

G

H

A

Q

K

M

L

V

-

R

-

G

-

S

-

L

-

E

-

S

-

L

-

H

-

L

-

A

A

S

V

I

F

F

S

S

T

T

D

Y

P

P

-

A

A

A

I

L

N

S

S

V

P

W

L

Q

S

A

N

A

F

L

V

V

S

E

E

V

I

V

I

I

G

T

T

S

F

I

M

L

-

M

-

G

L

M

V

I

M

M

G

A

L

L

L

T

F

D

I

D

G

G

A

N

N

G

E

V

F

P

T

K

Q

G

G

P

L

L

N

A

P

P

F

L

I

L

V

I

G

G

L

I

L

L

I

V

V

A

A

V

I

I

G

G

M

A

S

S

L

T

G

G

G

P

A

L

T

T

G

G

Y

F

A

A

I

M

N
|
N

P
|
P

A
|
A

R

R

D

D

I

F

G

G

P

P

R

K

I

L

A

F

H

A

M

W

L

M

L

A

-

G

-

W

-

G

P

D

I

V

P

A

G

M

K

T

G

G

S

R

N

D

W

I

G

P

Y

Y

A

F

W

I

V

V

P

P

L

I

F

V

G

A

P

P

I

I

V

I

G

G

G

A

V

C

Y

A

G

G

A

A

L

A

F

I

Y

Y

K

R

NPA 66:68 (= NPA 64:66) NPA 1
NPA 201:203 (= NPA 185:187) NPA 2

8jy8A Structure of tbaqp2 in complex with anti-trypanosomatid drug pentamidine (see paper)
32% identity, 83% coverage: 2:231/276 of query aligns to 2:237/242 of 8jy8A

query
sites
8jy8A

T

R

E

D

F

Y

L

V

A

A

E

E

L

F

I

L

G

G

T

N

M

F

I

V

L

L

I

I

I

Y

F

-

G

-

V

I

V

A

G

K

G

G

V

A

V

V

L

I

K

T

K

S

S

L

K

L

A

V

E

P

G

D

T

F

G

G

W

L

V

L

L

G

I

L

T

T

L

I

G

G

W

I

G

G

L

V

A

A

V

V

T

T

M

M

G

A

V

L

Y

Y

A

V

V

S

G

L

N

G

V

I

S

S

G

G

A

G

H

H

I

L

N

N

P

S

A

A

V

V

T

T

I

V

G

G

F

N

A

A

M

V

I

F

G

G

E

D

F

F

P

P

W

W

S

R

Q

K

V

V

P

P

T

G

F

Y

L

I

A

A

G

A

Q

Q

F

M

I

L

G

G

A

T

F

F

L

L

G

G

G

A

V

A

I

C

-

A

-

Y

-

G

V

V

F

F

L

A

N

D

Y

L

L

L

P

K

H

A

W

H

N

G

A

G

T

G

E

E

-

L

-

I

-

A

-

F

-

G

D

E

K

K

G

G

A

I

K

A

L

W

A

-

V

V

F

F

S

A

T

M

D

Y

P

P

A

A

I

E

-

G

N

N

S

G

P

I

L

F

S

Y

N

P

F

I

V

F

S

A

E

E

I

L

I

I

G

S

T

T

F

A

M

V

L

L

V

L

M

L

G

C

L

V

-

C

-

G

L

I

F

F

I

D

G

P

A

N

N

N

E

S

F

P

T

A

Q

K

G

G

L

Y

N

E

P

T

F

V

I

A

V

I

G

G

L

A

L

L

I
x
V

V

F

A

V

I

M

G

V

M

N

S

N

L

F

G

G

G

L

A

A

T

S

G

P

Y

L

A
|
A

I

M

N
|
N

P

P

A

S

R
x
L

D

D

I

F

G

G

P

P

R

R

I

V

A

F

H

G

M

A

L

I

L

L

P

-

I

-

P

-

G

-

K

L

G

G

S

E

N

V

W

F

G

S

Y

H

A

A

-

N

-

Y

-

F

W

W

V

V

P

P

L

L

F

V

G

V

P

P

I

F

V

F

G

G

G

A

V

I

Y

L

G

G

A

L

L

F

F

L

Y

Y

K

K

binding 1,5-bis(4-amidinophenoxy)pentane: V175 (≠ I169), A189 (= A183), N191 (= N185), L194 (≠ R188)

8jy6A Structure of tbaqp2 in complex with anti-trypanosomatid drug melarsoprol (see paper)
32% identity, 83% coverage: 2:231/276 of query aligns to 2:237/242 of 8jy6A

query
sites
8jy6A

T

R

E

D

F

Y

L

V

A

A

E

E

L

F

I

L

G

G

T

N

M

F

I

V

L

L

I

I

I

Y

F

-

G

-

V

I

V

A

G

K

G

G

V

A

V

V

L

I

K

T

K

S

S

L

K

L

A

V

E

P

G

D

T

F

G

G

W

L

V

L

L

G

I

L

T

T

L

I

G

G

W

I

G

G

L

V

A

A

V

V

T

T

M

M

G

A

V

L

Y

Y

A

V

V

S

G

L

N

G

V

I

S

S

G

G

A

G

H
|
H

I
x
L

N

N

P

S

A

A

V

V

T

T

I

V

G

G

F

N

A

A

M

V

I

F

G

G

E

D

F

F

P

P

W

W

S

R

Q

K

V

V

P

P

T

G

F

Y

L

I

A

A

G

A

Q

Q

F

M

I

L

G

G

A

T

F

F

L

L

G

G

G

A

V

A

I

C

-

A

-

Y

-

G

V

V

F

F

L

A

N

D

Y

L

L

L

P

K

H

A

W

H

N

G

A

G

T

G

E

E

-

L

-

I

-

A

-

F

-

G

D

E

K

K

G

G

A

I

K

A

L

W

A

-

V

V

F

F

S

A

T
x
M

D

Y

P

P

A

A

I

E

-

G

N

N

S

G

P

I

L

F

S

Y

N

P

F

I

V

F

S

A

E

E

I

L

I

I

G

S

T

T

F

A

M

V

L

L

V

L

M

L

G

C

L

V

-

C

-

G

L

I

F

F

I

D

G

P

A

N

N

N

E

S

F

P

T

A

Q

K

G

G

L

Y

N

E

P

T

F

V

I

A

V
x
I

G

G

L

A

L

L

I
x
V

V

F

A

V

I

M

G
x
V

M

N

S

N

L

F

G

G

G

L

A

A

T

S

G

P

Y

L

A
|
A

I

M

N
|
N

P

P

A

S

R
x
L

D

D

I

F

G

G

P

P

R

R

I

V

A

F

H

G

M

A

L

I

L

L

P

-

I

-

P

-

G

-

K

L

G

G

S

E

N

V

W

F

G

S

Y

H

A

A

-

N

-

Y

-

F

W

W

V

V

P

P

L

L

F

V

G

V

P

P

I

F

V

F

G

G

G

A

V

I

Y

L

G

G

A

L

L

F

F

L

Y

Y

K

K

binding [(4~{R})-2-[4-[[4,6-bis(azanyl)-1,3,5-triazin-2-yl]amino]phenyl]-1,3,2-dithiarsolan-4-yl]methanol: H58 (= H62), L59 (≠ I63), M126 (≠ T123), I171 (≠ V165), V175 (≠ I169), V179 (≠ G173), A189 (= A183), N191 (= N185), L194 (≠ R188)

Q6Z2T3 Aquaporin NIP2-1; Low silicon protein 1; NOD26-like intrinsic protein 2-1; OsNIP2;1; Silicon influx transporter LSI1 from Oryza sativa subsp. japonica (Rice) (see paper)
29% identity, 84% coverage: 1:233/276 of query aligns to 48:257/298 of Q6Z2T3

query
sites
Q6Z2T3

M

L

T

K

E

K

F

V

L

V

A

S

E

E

L

V

I

V

G

A

T

T

M

F

I

L

L

L

I

V

I

F

F

M

G

T

G

C

G

G

V

A

G

G

G

I

V

S

V

G

L

S

K

D

K

L

S

S

K

R

A

I

E

S

G

Q

T

L

G

G

W

Q

V

-

L

-

I

-

T

S

L

I

G

A

W

G

G

G

L

L

A

I

V

V

T

T

M

V

G

M

V

I

Y

Y

A

A

V

V

G

G

N

H

V

I

S

S

G

G

A

A

H

H

I

M

N

N

P

P

A

A

V

V

T

T

I

L

G

A

F

F

A

A

M

V

I

F

G

R

E

H

F

F

P

P

W

W

S

I

Q

Q

V

V

P

P

T

F

F

Y

L

W

A

A

G
x
A

Q

Q

F

F

I

T

G

G

A

A

F

I

L

C

G

A

G

S

V

F

I

V

V

L

F

K

L

A

N

V

Y

I

L

H

P

P

H

-

W

-

N

-

A

-

T

-

E

-

D

-

K

-

G

-

A

-

K

-

L

V

A

D

V

V

F

I

S

G

T

T

D

T

P

T

A

P

I

V

N

G

S

P

P

H

L

W

S

H

N

S

F

L

V

V

S

V

E

E

I

V

I

I

G

V

T

T

F

F

M

N

L

M

V

M

M

F

G

V

L

T

L

L

F

A

I

V

G

A

A

T

N

D

E

T

F

R

T

A

Q

V

G

G

-

E

L

L

N

A

P

G

F

L

I

A

V

V

G

G

L

S

L

A

I

V

V

C

A

I

I

T

G

S

M

I

S

F

L

A

G

G

G

A

A

I

T

S

G

G

Y

G

A

S

I

M

N

N

P

P

A

A

R

R

D

T

I

L

G

G

P

P

R

A

I

L

A

A

H

-

M

-

L

-

P

-

I

-

P

-

G

-

K

-

G

-

G

S

S

N

N

K

W

F

G

D

Y

G

A

L

W

W

V

I

P

Y

L

F

F

L

G

G

P

P

I

V

V

M

G

G

G

T

V

L

Y

S

G

G

A

A

L

W

F

T

Y

Y

K

T

A

F

I

I

A132 (≠ G88) mutation to T: In lsi; impairs silicon uptake. Grain discoloration. Reduces grain yield 10-fold.

P08995 Nodulin-26; N-26 from Glycine max (Soybean) (Glycine hispida) (see paper)
29% identity, 84% coverage: 1:233/276 of query aligns to 37:247/271 of P08995

query
sites
P08995

M

L

T

Q

E

K

F

L

L

V

A

A

E

E

L

A

I

V

G

G

T

T

M

Y

I

F

L

L

I

I

I

F

F

A

G

G

G

-

V

-

V

C

G

A

G

S

V

L

V

V

L

V

K

N

K

E

S

N

K

Y

A

Y

E

N

G

M

T

I

G

T

W

F

V

P

L

G

I

I

T

A

L

I

G

V

W

W

G

G

L

L

A

V

V

L

T

T

M

V

G

L

V

V

Y

Y

A

T

V

V

G

G

N

H

V

I

S

S

G

G

A

G

H

H

I

F

N

N

P

P

A

A

V

V

T

T

I

I

G

A

F

F

A

A

M

S

I

T

G

R

E

R

F

F

P

P

W

L

S

I

Q

Q

V

V

P

P

T

A

F

Y

L

V

A

V

G

A

Q

Q

F

L

I

L

G

G

A

S

F

I

L

L

G

A

G

S

-

G

-

T

-

L

V

R

I

L

V

L

F

F

L

M

N

G

Y

N

L

H

P

D

H

Q

W

F

N

S

A

G

T

T

E

V

D

P

K

N

G

G

A

T

K

N

L

L

A

Q

V

A

F

-

S

-

T

-

D

-

P

-

A

-

I

-

N

-

S

-

P

-

L

-

S

-

N

-

F

F

V

V

S

F

E

E

I

F

I

I

G

M

T

T

F

F

M

F

L

L

V

M

M

F

G

V

L

I

L

C

F

G

I

V

G

A

A

T

N

D

E

N

F

R

T

A

Q

V

G

G

L

-

N

-

P

-

F

-

I

-

V

-

G

E

L

F

L

A

I

G

V

I

A

A

I

I

G

G

M

S

S

T

L

L

-

L

-

N

-

V

-

I

-

G

G

G

G

P

A

V

T

T

G

G

Y

A

A

S

I

M

N

N

P

P

A

A

R

R

D

S

I

L

G

G

P

P

R

A

I

F

A

V

H

H

M

-

L

-

P

-

I

-

P

-

G

-

K

-

G

-

S

G

N

E

W

Y

G

E

Y

G

A

I

W

W

V

I

P

Y

L

L

F

L

G

A

P

P

I

V

V

V

G

G

G

A

V

I

Y

A

G

G

A

A

L

W

F

V

Y

Y

K

N

A

I

I

V

Sites not aligning to the query:

262 modified: Phosphoserine; by CPK

Q9SAI4 Aquaporin NIP6-1; NOD26-like intrinsic protein 6-1; AtNIP6;1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
31% identity, 82% coverage: 6:230/276 of query aligns to 84:285/305 of Q9SAI4

query
sites
Q9SAI4

A

A

E

E

L

F

I

V

G

G

T

T

M

L

I

I

L

L

I

I

I

-

F

F

G

A

G

G

G

T

V

A

V

T

G

A

G

I

V

V

V

N

L

Q

K

K

K

T

S

D

K

G

A

A

E

E

G

T

T

-

G

-

W

L

V

I

L

G

I

C

T

A

L

A

G

S

W
x
A

G

G

L

L

A

A

V

V

T

M

M

I

G

V

V

I

Y

L

A

S

V

T

G

G

N

H

V

I

S

S

G

G

A

A

H

H

I

L

N

N

P

P

A

A

V

V

T

T

I

I

G

A

F

F

A

A

M

A

I

L

G

K

E

H

F

F

P

P

W

W

S

K

Q

H

V

V

P

P

T

V

F

Y

L

I

A

G

G

A

Q

Q

F

V

I

M

G

A

A

S

F

V

L

S

G

A

V

F

I

A

V

L

F

K

L

A

N

V

Y

F

L

E

P

P

H

-

W

-

N

-

A

-

T

T

E

M

D

S

K

G

G

G

A

V

K

T

L

V

A

P

V

T

F

V

S

G

T

L

D

S

P

Q

A

A

I

-

N

-

S

-

P

-

L

-

S

-

N

-

F

F

V

A

S

L

E

E

I

F

I

I

G

I

T

S

F

F

M

N

L

L

V

M

M

F

G

V

L

V

L

T

F

A

I

V

G

A

A

T

N

D

E

T

F

R

T

A

Q

V

G

G

-

E

L

L

N

A

P

G

F

I

I

A

V

V

G

G

L

A

L

T

I

V

V

M

A

L

I

N

G

I

M

L

S

I

L

A

G

G

G

P

A

A

T

T

G

S

Y

A

A

S

I

M

N

N

P

P

A
x
V

R

R

D

T

I

L

G

G

P

P

R

A

I

I

A

A

H

-

M

-

L

-

P

-

I

-

P

-

G

-

K

-

G

-

G

A

S

N

N

N

W

Y

G

R

Y

A

A

I

W

W

V

V

P

Y

L

L

F

T

G

A

P

P

I

I

V

L

G

G

G

A

V

L

Y

I

G

G

A

A

L

G

F

T

Y

Y

A119 (≠ W44) mutation to W: 6-fold increase in water transport activity, but impaired in urea transport.
V252 (≠ A187) mutation to A: No effect.

P47863 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Rattus norvegicus (Rat) (see 4 papers)
28% identity, 72% coverage: 38:237/276 of query aligns to 71:255/323 of P47863

query
sites
P47863

V

V

L

L

I

I

T

S

L

L

G

C

W

F

G

G

L

L

A

S

V

I

T

A

M

T

G

M

V

V

Y

Q

A

C

V

F

G

G

N

H

V

I

S

S

G

G

A

G

H

H

I

I

N

N

P

P

A

A

V

V

T

T

I

V

G

A

F

M

A

V

M

C

I

T

G

R

E

K

F

I

P

S

W

I

S

A

Q

K

V

S

P

V

T

F

F

Y

L

I

A

T

G

A

Q

Q

F

C

I

L

G

G

A

A

F

I

L

I

G

G

G

A

V

G

I

I

V

L

F

Y

L

L

N

V

Y

T

L

P

P

P

-

S

-

V

-

G

-

L

-

G

-

V

-

T

-

V

H

H

W

G

N

N

A

L

T

T

E

A

D

G

K

H

G

G

A

-

K

-

L

-

A

-

V

-

F

-

S

-

T

-

D

-

P

-

A

-

I

-

N

-

S

-

P

-

L

-

S

-

N

-

F

L

V

L

S

V

E

E

I

L

I

I

G

I

T

T

F

F

M

Q

L

L

V

V

M

F

G

T

L

I

L

F

F

A

I

S

G

C

A

D

N
x
S

E

K

F

R

T

T

Q

D

G

-

L

-

N

-

P

-

F

-

I

V

V

T

G

G

L

S

L

V

I

A

V

L

A

A

I

I

G

G

M

F

S

S

L

V

G

A

-

I

-

G

-
x
H

-

L

-

F

-

A

-

I

G

N

A

Y

T

T

G

G

Y

A

A

S

I

M

N

N

P

P

A

A

R

R

D

S

I

F

G

G

P

P

R

A

I

V

A

-

H

-

M

-

L

-

P

-

I

I

P

M

G

G

K

-

G

-

S

-

N

N

W

W

G

E

Y

N

A

H

W

W

V

I

P

Y

L

W

F

V

G

G

P

P

I

I

V

I

G

G

G

A

V

V

Y

L

G

A

A

G

L

A

F

L

Y

Y

K

E

A

Y

I

V

F

F

T

C

G

P

E

D

S180 (≠ N154) modified: Phosphoserine; by PKC; mutation to A: Decreases internalization from the cell membrane in response to PKC activation.
H201 (vs. gap) mutation to P: Partial loss of transport activity.

Sites not aligning to the query:

13 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-17.
17 modified: S-palmitoyl cysteine; C→A: Reduced palmitoylation. Loss of palmitoylation; when associated with A-13.
285 modified: Phosphoserine

P55088 Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4 from Mus musculus (Mouse) (see 2 papers)
27% identity, 83% coverage: 6:235/276 of query aligns to 40:253/323 of P55088

query
sites
P55088

A

A

E

E

L

F

I

L

G

A

T

T

M

L

I

I

L

F

I

V

I

L

F

L

G

G

G

V

G

G

V

S

V

T

-

I

-

N

-

W

G

G

V

-

L

-

K

-

K

S

S

E

K

N

A

P

E

L

G

P

T

V

G

D

W

M

V

V

L

L

I

I

T

S

L

L

G

C

W

F

G

G

L

L

A

S

V

I

T

A

M

T

G

M

V

V

Y

Q

A

C

V

F

G

G

N

H

V

I

S

S

G

G

A

G

H

H

I

I

N

N

P

P

A

A

V

V

T

T

I

V

G

A

F

M

A

V

M

C

I

T

G

R

E

K

F

I

P
x
S

W

I

S

A

Q

K

V

S

P

V

T

F

F

Y

L

I

A

I

G

A

Q

Q

F

C

I

L

G

G

A

A

F

I

L

I

G

G

G

A

V

G

I

I

V

L

F

Y

L

L

N

V

Y

T

L

P

P

P

-

S

-

V

-

G

-

L

-

G

-

V

-

T

-

V

H

H

W

G

N

N

A

L

T

T

E

A

D

G

K

H

G

G

A

-

K

-

L

-

A

-

V

-

F

-

S

-

T

-

D

-

P

-

A

-

I

-

N

-

S

-

P

-

L

-

S

-

N

-

F

L

V

L

S

V

E

E

I

L

I

I

G

I

T

T

F

F

M

Q

L

L

V

V

M

F

G

T

L

I

L

F

F

A

I

S

G

C

A

D

N

S

E

K

F

R

T

T

Q

D

G

-

L

-

N

-

P

-

F

-

I

V

V

T

G

G

L

S

L

I

I

A

V

L

A

A

I

I

G

G

M

F

S

S

L

V

G

A

-

I

-

G

-

H

-

L

-

F

-

A

-

I

G

N

A

Y

T

T

G

G

Y

A

A

S

I

M

N

N

P

P

A

A

R

R

D

S

I

F

G

G

P

P

R

A

I

V

A

-

H

-

M

-

L

-

P

-

I

I

P

M

G

G

K

-

G

-

S

-

N

N

W

W

G

A

Y

N

A

H

W

W

V

I

P

Y

L

W

F

V

G

G

P

P

I

I

V

M

G

G

G

A

V

V

Y

L

G

A

A

G

L

A

F

L

Y

Y

K

E

A

Y

I

V

F

F

T

C

S111 (≠ P78) modified: Phosphoserine; by PKG; mutation to A: Loss of phosphorylation by PKG (in vitro). No effect on location at cell membrane.

Sites not aligning to the query:

4 natural variant: G -> R

P41181 Aquaporin-2; AQP-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct from Homo sapiens (Human) (see 12 papers)
30% identity, 83% coverage: 6:234/276 of query aligns to 15:223/271 of P41181

query
sites
P41181

A

A

E

E

L

F

I

L

G

A

T

T

M

L

I

L

L

F

I

V

I

F

F

F

G

G

G

L

G

G

V

S

V

A

G

-

V

-

L

L

K

N

K

W

S

P

K

Q

A

A

E

L

G

P

T

S

G

-

W

V

V

L

L

Q

I

I

T

A

L

M

G

A

W

F

G

G

L

L

A

G

V

I

T

G

M

T

G

L

V

V

Y

Q

A

A

V

L

G

G

N

H

V

I

S

S

G
|
G

A

A

H

H

I

I

N

N

P

P

A

A

V

V

T

T

I

V

G

A

F

C

A

L

M

V

I
x
G

G
x
C

E

H

F

V

P

S

W

V

S

L

Q

R

V

A

P

A

T

F

F

Y

L

V

A

A

G

A

Q

Q

F

L

I

L

G

G

A

A

F

V

L

A

G

G

G

A

V

A

I

L

V

L

F

-

L

-

N

-

Y

-

L

-

P

-

H

H

W

E

N

I

A

T

T
&