SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_036835323.1 NCBI__GCF_000775615.1:WP_036835323.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P70994 2-hydroxymuconate tautomerase; (2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Bacillus subtilis (strain 168) (see paper)
80% identity, 98% coverage: 1:60/61 of query aligns to 1:60/62 of P70994

query
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P70994

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P2 (= P2) active site, Proton acceptor; via imino nitrogen; mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.
R12 (= R12) mutation to A: Absence of tautomerase activity using 2-hydroxymuconate. For 2-hydroxy-2,4-pentadienoate and phenylenolpyruvate, a strong decrease of catalytic efficiency and a 2-fold decrease of affinity is observed.

2opaA Ywhb binary complex with 2-fluoro-p-hydroxycinnamate
80% identity, 97% coverage: 2:60/61 of query aligns to 1:59/61 of 2opaA

query
sites
2opaA

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active site: V39 (= V40), Y50 (= Y51)
binding 2-fluoro-3-(4-hydroxyphenyl)-2e-propeneoate: M7 (= M8), R11 (= R12), Y50 (= Y51)

Sites not aligning to the query:

active site: 61

Q01468 2-hydroxymuconate tautomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
43% identity, 92% coverage: 1:56/61 of query aligns to 1:56/63 of Q01468

query
sites
Q01468

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M1 (= M1) modified: Initiator methionine, Removed
P2 (= P2) active site, Proton acceptor; via imino nitrogen

5tigA Crystal structure of 4-oxalocrotonate tautomerase inactivated by brhpd (see paper)
42% identity, 90% coverage: 2:56/61 of query aligns to 1:55/62 of 5tigA

query
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5tigA

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active site: P1 (= P2), R39 (≠ V40), F50 (≠ Y51)
binding (3E)-5-hydroxy-2-oxopent-3-enoic acid: F50 (≠ Y51)

Sites not aligning to the query:

active site: 61
binding (3E)-5-hydroxy-2-oxopent-3-enoic acid: 61

1bjpA Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2- oxo-3-pentynoate at 2.4 angstroms resolution (see paper)
42% identity, 90% coverage: 2:56/61 of query aligns to 1:55/62 of 1bjpA

query
sites
1bjpA

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active site: P1 (= P2), R11 (= R12), R39 (≠ V40), F50 (≠ Y51)
binding 2-oxo-3-pentenoic acid: P1 (= P2), I2 (≠ Y3), F50 (≠ Y51)

Sites not aligning to the query:

binding 2-oxo-3-pentenoic acid: 61

6ghwC Substituting the prolines of 4-oxalocrotonate tautomerase with non- canonical analogue (2s)-3,4-dehydroproline (see paper)
42% identity, 90% coverage: 2:56/61 of query aligns to 1:55/57 of 6ghwC

query
sites
6ghwC

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active site: P1 (= P2), R39 (≠ V40), F50 (≠ Y51)
binding calcium ion: R29 (≠ E30), D32 (≠ G33)

6bgnA Crystal structure of 4-oxalocrotonate tautomerase after incubation with 5-fluoro-2-hydroxy-2,4-pentadienoate (see paper)
41% identity, 95% coverage: 2:59/61 of query aligns to 1:58/59 of 6bgnA

query
sites
6bgnA

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active site: P1 (= P2), R39 (≠ V40), F50 (≠ Y51)
binding 5-fluoranyl-2-oxidanylidene-pentanoic acid: P1 (= P2), S37 (≠ K38)

5cloM Crystal structure of a 4-oxalocrotonate tautomerase mutant in complex with nitrostyrene at 2.3 angstrom (see paper)
40% identity, 90% coverage: 2:56/61 of query aligns to 1:55/57 of 5cloM

query
sites
5cloM

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active site: P1 (= P2), R39 (≠ V40), A50 (≠ Y51)
binding trans beta nitrostyrene: P1 (= P2), S37 (≠ K38)

3ej3C Structural and mechanistic analysis of trans-3-chloroacrylic acid dehalogenase activity (see paper)
32% identity, 97% coverage: 2:60/61 of query aligns to 1:59/64 of 3ej3C

query
sites
3ej3C

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binding acetate ion: R8 (≠ L9), R11 (= R12)

Query Sequence

>WP_036835323.1 NCBI__GCF_000775615.1:WP_036835323.1
MPYVTVKMLEGRTDEQKKALVEKVTEAVSETSGAPKEKVVVFLEDMPKNHYAVGGKRLSD
Q

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory