SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_037374637.1 NCBI__GCF_000428045.1:WP_037374637.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

D2Z027 O-ureido-L-serine synthase; Cysteine synthase homolog DscD; O-acetylserine sulfhydrylase; EC 2.6.99.3; EC 2.5.1.47 from Streptomyces lavendulae (see paper)
51% identity, 99% coverage: 3:302/303 of query aligns to 4:303/324 of D2Z027

query
sites
D2Z027

Y

F

D

N

N

S

I

I

L

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

V

I

I

V

R

R

L

L

N

Q

R

R

F

M

-

A

P

P

D

E

T

H

L

-

A

-

A

T

E

S

L

V

W

Y

V

V

K

K

L

V

E

E

S

S

F

F

N

N

P

P

A

G

G

G

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

L

N

S

M

V

I

V

E

L

C

D

A

A

E

E

Q

A

R

K

G

G

E

L

L

L

K

K

P

P

G

G

D

D

T

T

I

I

I

V

E

E

P

C

T

T

S

S

G

G

N

N

T
x
V

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

F

V

V

F

A

V

V

M

M

A

G

E

D

D

T

M
x
Y

S

S

E

V

E

E

R

R

K

R

T

K

I

L

L

I

R

R

A

A

F

Y

G

G

K

K

L

L

V

V

L

L

T

F

P

P

A

G

E

H

K

L

G

G

T
x
S

K

K

G

G

A

G

I

N

E

L

E

I

A

A

K

D

K

E

L

L

A

A

A

E

K

K

N

Y

G

G

W

W

F

F

F

R

V

A

G

R

Q

Q

H

F

F

D

N

N

P

P

D

A

N

N

P

P

N

S

A

Y

H

H

K

R

-

E

G

T

T

T

A

A

D

S

E

E

I

I

W

L

A

A

D

D

F

F

-

A

G

G

T

K

R

R

L

L

D

D

A

H

I

F

V

V

C

T

T

G

T

F

G

G

T

T

G

T

G

G

T

T

I

L

S

T

G

G

I

V

G

G

K

Q

Y

M

M

L

R

R

R

V

H

A

N

R

P

P

E

E

I

V

Q

R

I

V

A

A

T

L

E

E

P

P

A

S

D

N

S

A

P

A

I

M

L

L

S

A

Q

R

G

G

I

E

A

W

C

S

K

P

H

H

K

Q

I

I

M

Q

G

G

T

L

A

A

P

P

G

N

F

F

I

V

P

P

D

G

I

V

L

L

D

D

T

R

K

S

I

V

Y

I

N

D

R

D

I

L

F

V

Q

T

I

M

T

D

T

E

D

V

E

T

A

A

Y

R

D

D

T

T

T

S

R

R

L

L

A

A

Q

A

H

E

E

E

G

G

I

I

F

F

A

A

G

G

I

I

S

S

S

A

G

G

A

A

A

T

V

V

A

A

G

T

M

A

I

L

K

S

A

I

A

A

R

-

E

E

E

H

A

A

F

P

R

E

G

G

K

T

I

V

I

L

L

L

A

A

M

M

L

L

P

P

D

D

T

T

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

F

L

L

W

F

K43 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Loss of catalytic activity, no longer binds N6-(pyridoxal phosphate)lysine.
V74 (≠ T74) mutation to T: KM for OAS is 61 mM, KM for H(2)S is unchanged.
Y97 (≠ M97) mutation to F: KM for OAS is unchanged, KM for H(2)S is 0.073 mM.; mutation to M: KM for OAS is 330 mM, KM for H(2)S is 0.084.
S121 (≠ T121) mutation to A: KM for OAS is 140 mM, KM for H(2)S is 0.095 mM.; mutation to M: KM for OAS is 44 mM, KM for H(2)S is 0.20 mM.

3x43A Crystal structure of o-ureido-l-serine synthase (see paper)
51% identity, 99% coverage: 3:302/303 of query aligns to 3:302/316 of 3x43A

query
sites
3x43A

Y

F

D

N

N

S

I

I

L

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

V

I

I

V

R

R

L

L

N

Q

R

R

F

M

-

A

P

P

D

E

T

H

L

T

A

S

A

-

E

-

L

V

W

Y

V

V

K

K

L

V

E

E

S

S

F

F

N

N

P

P

A

G

G

G

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

L

N

S

M

V

I

V

E

L

C

D

A

A

E

E

Q

A

R

K

G

G

E

L

L

L

K

K

P

P

G

G

D

D

T

T

I

I

I

V

E

E

P

C

T

T

S

S

G

G

N
|
N

T

V

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

F

V

V

F

A

V

V

M

M

A

G

E

D

D

T

M

Y

S

S

E

V

E

E

R

R

K

R

T

K

I

L

L

I

R

R

A

A

F

Y

G

G

K

K

L

L

V

V

L

L

T

F

P

P

A

G

E

H

K

L

G

G

T

S

K

K

G

G

A

G

I

N

E

L

E

I

A

A

K

D

K

E

L

L

A

A

A

E

K

K

N

Y

G

G

W

W

F

F

F

R

V

A

G

R

Q

Q

H

F

F

D

N

N

P

P

D

A

N

N

P

P

N

S

A

Y

H

H

K

R

-

E

G

T

T

T

A

A

D

S

E

E

I

I

W

L

A

A

D

D

F

F

-

A

G

G

T

K

R

R

L

L

D

D

A

H

I

F

V

V

C

T

T

G

T
x
F

G
|
G

T
|
T

G
x
T

G

G

T
|
T

I

L

S

T

G

G

I

V

G

G

K

Q

Y

M

M

L

R

R

R

V

H

A

N

R

P

P

E

E

I

V

Q

R

I

V

A

A

T

L

E

E

P

P

A

S

D

N

S

A

P

A

I

M

L

L

S

A

Q

R

G

G

I

E

A

W

C

S

K

P

H

H

K

Q

I

I

M

Q

G
|
G

T

L

A

A

P

P

G

N

F

F

I

V

P

P

D

G

I

V

L

L

D

D

T

R

K

S

I

V

Y

I

N

D

R

D

I

L

F

V

Q

T

I

M

T

D

T

E

D

V

E

T

A

A

Y

R

D

D

T

T

T

S

R

R

L

L

A

A

Q

A

H

E

E

E

G

G

I

I

F

F

A

A

G

G

I

I

S
|
S

S

A

G

G

A

A

A

T

V

V

A

A

G

T

M

A

I

L

K

S

A

I

A

A

R

-

E

E

E

H

A

A

F

P

R

E

G

G

K

T

I

V

I

L

L

L

A

A

M

M

L

L

P
|
P

D
|
D

T

T

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

F

L

L

W

F

active site: K42 (= K43), S264 (= S263)
binding pyridoxal-5'-phosphate: K42 (= K43), N72 (= N73), F175 (≠ T174), G176 (= G175), T177 (= T176), T178 (≠ G177), T180 (= T179), G220 (= G219), S264 (= S263), P290 (= P290), D291 (= D291)

3x44A Crystal structure of o-ureido-l-serine-bound k43a mutant of o-ureido- l-serine synthase (see paper)
50% identity, 99% coverage: 3:302/303 of query aligns to 3:302/321 of 3x44A

query
sites
3x44A

Y

F

D

N

N

S

I

I

L

L

Q

D

T

T

I

I

G

G

N

R

T

T

P

P

V

I

I

V

R

R

L

L

N

Q

R

R

F

M

-

A

P

P

D

E

T

H

L

T

A

S

A

-

E

-

L

V

W

Y

V

V

K

K

L

V

E

E

S

S

F

F

N

N

P

P

A

G

G

G

S

S

V

V

K
x
A

D

D

R

R

P

L

A

A

L

L

N

S

M

V

I

V

E

L

C

D

A

A

E

E

Q

A

R

K

G

G

E

L

L

L

K

K

P

P

G

G

D

D

T

T

I

I

I

V

E

E

P

C

T
|
T

S
|
S

G

G

N
|
N

T
x
V

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

F

V

V

F

A

V

V

M

M

A

G

E

D

D

T

M

Y

S

S

E

V

E

E

R

R

K

R

T

K

I

L

L

I

R

R

A

A

F

Y

G

G

K

K

L

L

V

V

L

L

T

F

P

P

A

G

E

H

K

L

G

G

T
x
S

K

K

G

G

A

G

I

N

E

L

E

I

A

A

K

D

K

E

L

L

A

A

A

E

K

K

N

Y

G

G

W

W

F

F

F

R

V

A

G

R

Q
|
Q

H

F

F

D

N

N

P

P

D

A

N

N

P

P

N

S

A

Y

H

H

K

R

-

E

G

T

T

T

A

A

D

S

E

E

I

I

W

L

A

A

D

D

F

F

-

A

G

G

T

K

R

R

L

L

D

D

A

H

I

F

V

V

C

T

T

G

T
x
F

G
|
G

T
|
T

G
x
T

G

G

T
|
T

I

L

S

T

G

G

I

V

G

G

K

Q

Y

M

M

L

R

R

R

V

H

A

N

R

P

P

E

E

I

V

Q

R

I

V

A

A

T

L

E

E

P

P

A

S

D

N

S

A

P

A

I

M

L

L

S

A

Q

R

G

G

I

E

A

W

C

S

K

P

H

H

K

Q

I

I

M

Q

G
|
G

T
x
L

A

A

P
|
P

G

N

F

F

I

V

P

P

D

G

I

V

L

L

D

D

T

R

K

S

I

V

Y

I

N

D

R

D

I

L

F

V

Q

T

I

M

T

D

T

E

D

V

E

T

A

A

Y

R

D

D

T

T

T

S

R

R

L

L

A

A

Q

A

H

E

E

E

G

G

I

I

F

F

A

A

G

G

I

I

S
|
S

S

A

G

G

A

A

A

T

V

V

A

A

G

T

M

A

I

L

K

S

A

I

A

A

R

-

E

E

E

H

A

A

F

P

R

E

G

G

K

T

I

V

I

L

L

L

A

A

M

M

L

L

P
|
P

D
|
D

T

T

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

F

L

L

W

F

active site: A42 (≠ K43), S264 (= S263)
binding (E)-O-(carbamoylamino)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: T69 (= T70), S70 (= S71), N72 (= N73), V73 (≠ T74), S120 (≠ T121), Q141 (= Q142), F175 (≠ T174), G176 (= G175), T177 (= T176), T178 (≠ G177), T180 (= T179), G220 (= G219), L221 (≠ T220), P223 (= P222), S264 (= S263), P290 (= P290), D291 (= D291)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 99% coverage: 4:303/303 of query aligns to 5:306/310 of P9WP55

query
sites
P9WP55

D

E

N

D

I

I

L

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

V

L

I

V

R

R

L

L

N

R

R

R

F

V

P

T

D

D

T

G

L

A

A

V

A

A

E

D

L

I

W

V

V

A

K

K

L

L

E

E

S

F

F

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

E

Q

C

A

A

A

E

E

Q

Q

R

A

G

G

E

L

L

I

K

K

P

P

G

D

D

T

T

I

I

I

I

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

C

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

C

V

V

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

L

E

E

R

R

K

R

T

M

I

L

L

L

R

R

A

A

F

Y

G

G

G

A

K

E

L

L

V

I

L

L

T

T

P

P

A

G

E

A

K

D

G

G

T

M

K

S

G

G

A

A

I

I

E

A

E

K

A

A

K

E

L

L

A

A

A

K

K

T

N

D

G

Q

W

R

F

Y

F

F

V

V

G

P

Q

Q

H

Q

F

F

-

E

N

N

P

P

D

A

N

N

P

P

N

A

A

I

H

H

K

R

-

V

G

T

T

T

A

A

D

E

E

E

I

V

W

W

A

R

D

D

F

T

G

D

T

G

R

K

L

V

D

D

A

I

I

V

V

V

C

A

T

G

T

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

T

G

G

I

V

G

A

K

Q

Y

V

M

I

R

K

R

E

H

R

N

K

P

P

E

S

I

A

Q

R

I

F

I

V

A

A

T

V

E

E

P

P

A

A

D

A

S

S

P

P

I

V

L

L

S

S

Q

G

G

G

I

Q

A

K

C

G

K

P

H

H

K

P

I

I

M

Q

G

G

T

I

A

G

P

A

G

G

F

F

I

V

P

P

D

P

I

V

L

L

D

D

T

Q

K

D

I

L

Y

V

N

D

R

E

I

I

F

I

Q

T

I

V

T

G

T

N

D

E

E

D

A

A

Y

L

D

N

T

V

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

T

A

V

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

R

E

P

A

E

F

N

R

A

G

G

K

K

I

L

I

I

L

V

A

V

M

V

L

L

P

P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

P

L

L

W

F

S

A

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 175:179) binding pyridoxal 5'-phosphate
S266 (= S263) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
46% identity, 99% coverage: 4:303/303 of query aligns to 5:306/306 of 2q3dA

query
sites
2q3dA

D

E

N

D

I

I

L

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

V

L

I

V

R

R

L

L

N

R

R

R

F

V

P

T

D

D

T

G

L

A

A

V

A

A

E

D

L

I

W

V

V

A

K

K

L

L

E

E

S

F

F

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

E

Q

C

A

A

A

E

E

Q

Q

R

A

G

G

E

L

L

I

K

K

P

P

G

D

D

T

T

I

I

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

C

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

C

V

V

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

L

E

E

R

R

K

R

T

M

I

L

L

L

R

R

A

A

F

Y

G

G

G

A

K

E

L

L

V

I

L

L

T

T

P

P

A

G

E

A

K

D

G

G

T

M

K

S

G

G

A

A

I

I

E

A

E

K

A

A

K

E

L

L

A

A

A

K

K

T

N

D

G

Q

W

R

F

Y

F

F

V

V

G

P

Q

Q

H
x
Q

F

F

-

E

N

N

P

P

D

A

N

N

P

P

N

A

A

I

H

H

K

R

-

V

G

T

T

T

A

A

D

E

E

E

I

V

W

W

A

R

D

D

F

T

G

D

T

G

R

K

L

V

D

D

A

I

I

V

V

V

C

A

T

G

T
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

I

V

G

A

K

Q

Y

V

M

I

R

K

R

E

H

R

N

K

P

P

E

S

I

A

Q

R

I

F

I

V

A

A

T

V

E

E

P

P

A

A

D

A

S

S

P

P

I

V

L

L

S

S

Q

G

G

G

I

Q

A

K

C

G

K

P

H

H

K

P

I

I

M

Q

G
|
G

T
x
I

A

G

P

A

G

G

F

F

I

V

P

P

D

P

I

V

L

L

D

D

T

Q

K

D

I

L

Y

V

N

D

R

E

I

I

F

I

Q

T

I

V

T

G

T

N

D

E

E

D

A

A

Y

L

D

N

T

V

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

T

A

V

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

R

E

P

A

E

F

N

R

A

G

G

K

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

P

L

L

W

F

S

A

active site: K44 (= K43), S266 (= S263), P293 (= P290)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (≠ H143), V177 (≠ T174), G178 (= G175), T179 (= T176), G180 (= G177), T182 (= T179), G222 (= G219), I223 (≠ T220), S266 (= S263), P293 (= P290), D294 (= D291)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
48% identity, 97% coverage: 8:302/303 of query aligns to 10:306/310 of 5xoqA

query
sites
5xoqA

Q

E

T

S

I

I

G

G

N

R

T

T

P

P

V

L

I

V

R

Q

L

I

N

N

R

R

F

L

P

T

D

A

T

G

L

L

A

S

E

R

L

V

W

L

V

A

K

K

L

I

E

E

S

G

F

R

N

N

P

P

A

A

G

Y

S

S

V

V

K

K

D

C

R

R

P

I

A

G

L

A

N

A

M

M

I

I

E

W

C

D

A

A

E

E

Q

Q

R

S

G

G

E

K

L

L

K

K

P

P

G

G

D

M

T

H

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

F

I

V

A

C

A

A

Q

A

K

R

G

G

Y

Y

P

K

S

L

V

T

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

I

E

E

R

R

K

R

T

M

I

M

L

L

R

K

A

S

F

F

G

G

G

A

K

D

L

L

V

V

L

L

T

T

P

P

A

G

E

A

K

D

G

G

T
x
M

K

K

G

G

A

A

I

I

E

S

E

K

A

A

K

E

L

L

A

A

K

Q

N

P

G

G

W

W

F

F

F

I

V

P

G

Q

Q
|
Q

H

F

F

K

N

N

P

P

D

A

N

N

P

P

N

A

A

I

H

H

-

V

K

K

G

T

T

T

A

G

D

P

E

E

I

I

W

W

A

N

D

D

F

T

G

E

T

G

R

Q

L

V

D

D

A

V

I

F

V

V

C

A

T

G

T

V

G

G

T

T

G

G

T

T

I

I

S

T

G

G

I

V

G

A

K

R

Y

F

M

L

R

K

R

H

H

E

N

K

P

K

E

H

-

P

I

V

Q

H

I

V

A

A

T

V

E

E

P

P

A

A

D

A

S

S

P

P

I

V

L

L

S

A

Q

G

G

G

I

P

A

A

C

G

K
x
R

H
|
H

K

K

I

I

M
x
Q

G
|
G

T

I

A

G

P
x
A

G

G

F

F

I

V

P

P

D

D

I

T

L

F

D

D

T

R

K

S

I

V

Y

V

N

D

R

E

I

I

F

L

Q

S

I

V

T

T

T

D

D

D

E

E

A

A

Y

I

D

E

T

T

T

A

R

R

R

K

L

L

A

A

Q

M

H

E

E

E

G

G

I

I

F

S

A

C

G

G

I

I

S

S

S

C

G

G

A

A

V

M

A

A

G

G

M

A

I

L

K

K

A

V

A

A

R

A

E

R

E

P

A

E

F

F

R

A

G

G

K

K

I

T

I

I

L

V

A

T

M

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

A

L

L

W

F

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (≠ T121), Q144 (= Q142), R218 (≠ K214), H219 (= H215), Q222 (≠ M218), G223 (= G219), A226 (≠ P222)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
46% identity, 97% coverage: 4:297/303 of query aligns to 5:300/300 of 3zeiA

query
sites
3zeiA

D

E

N

D

I

I

L

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

V

L

I

V

R

R

L

L

N

R

R

R

F

V

P

T

D

D

T

G

L

A

A

V

A

A

E

D

L

I

W

V

V

A

K

K

L

L

E

E

S

F

F

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

E

Q

C

A

A

A

E

E

Q

Q

R

A

G

G

E

L

L

I

K

K

P

P

G

D

D

T

T

I

I

I

I

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

C

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

C

V

V

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

L

E

E

R

R

K

R

T

M

I

L

L

L

R

R

A

A

F

Y

G

G

G

A

K

E

L

L

V

I

L

L

T

T

P

P

A

G

E

A

K

D

G

G

T

M

K

S

G

G

A

A

I
|
I

E

A

E

K

A

A

K

E

L

L

A

A

A

K

K

T

N

D

G

Q

W

R

F

Y

F

F

V

V

G

P

Q

Q

H
x
Q

F
|
F

-

E

N

N

P

P

D

A

N

N

P

P

N

A

A

I

H

H

K

R

-

V

G

T

T

T

A

A

D

E

E

E

I

V

W

W

A

R

D

D

F

T

G

D

T

G

R

K

L

V

D

D

A

I

I

V

V

V

C

A

T

G

T
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

I

V

G

A

K

Q

Y

V

M

I

R

K

R

E

H

R

N

K

P

P

E

S

I

A

Q

R

I

F

I

V

A

A

T

V

E

E

P

P

A

A

D

A

S

S

P

P

I

V

L

L

S

S

Q

G

G

G

I

Q

A
x
K

C

G

K

P

H

H

K

P

I

I

M

Q

G
|
G

T

I

A

G

P
x
A

G

G

F
|
F

I

V

P

P

D

P

I

V

L

L

D

D

T

Q

K

D

I

L

Y

V

N

D

R

E

I

I

F

I

Q

T

I

V

T

G

T

N

D

E

E

D

A

A

Y

L

D

N

T

V

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

T

A

V

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

R

E

P

A

E

F

N

R

A

G

G

K

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D
|
D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S263), P293 (= P290)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (= I125), Q144 (≠ H143), F145 (= F144), K215 (≠ A212), G222 (= G219), A225 (≠ P222), F227 (= F224)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (≠ T174), G178 (= G175), T179 (= T176), G180 (= G177), T182 (= T179), G222 (= G219), S266 (= S263), P293 (= P290), D294 (= D291)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
46% identity, 97% coverage: 4:297/303 of query aligns to 5:300/300 of 2q3cA

query
sites
2q3cA

D

E

N

D

I

I

L

T

Q

Q

T

L

I

I

G

G

N

R

T

T

P

P

V

L

I

V

R

R

L

L

N

R

R

R

F

V

P

T

D

D

T

G

L

A

A

V

A

A

E

D

L

I

W

V

V

A

K

K

L

L

E

E

S

F

F

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

N

A

M

M

I

L

E

Q

C

A

A

A

E

E

Q

Q

R

A

G

G

E

L

L

I

K

K

P

P

G

D

D

T

T

I

I

I

I

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

M

I

V

A

C

A

A

Q

A

K

R

G

G

Y

Y

P

R

S

C

V

V

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

L

E

E

R

R

K

R

T

M

I

L

L

L

R

R

A

A

F

Y

G

G

G

A

K

E

L

L

V

I

L

L

T

T

P

P

A

G

E

A

K

D

G

G

T
x
M

K

S

G

G

A

A

I

I

E

A

E

K

A

A

K

E

L

L

A

A

A

K

K

T

N

D

G

Q

W

R

F

Y

F

F

V

V

G

P

Q

Q

H
x
Q

F

F

-

E

N

N

P

P

D

A

N

N

P

P

N

A

A

I

H

H

K

R

-

V

G

T

T

T

A

A

D

E

E

E

I

V

W

W

A

R

D

D

F

T

G

D

T

G

R

K

L

V

D

D

A

I

I

V

V

V

C

A

T

G

T

V

G

G

T

T

G

G

T

T

I

I

S

T

G

G

I

V

G

A

K

Q

Y

V

M

I

R

K

R

E

H

R

N

K

P

P

E

S

I

A

Q

R

I

F

I

V

A

A

T

V

E

E

P

P

A

A

D

A

S

S

P

P

I

V

L

L

S

S

Q

G

G

G

I

Q

A
x
K

C

G

K

P

H

H

K

P

I

I

M

Q

G
|
G

T

I

A

G

P
x
A

G

G

F

F

I

V

P

P

D

P

I

V

L

L

D

D

T

Q

K

D

I

L

Y

V

N

D

R

E

I

I

F

I

Q

T

I

V

T

G

T

N

D

E

E

D

A

A

Y

L

D

N

T

V

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S
|
S

S

S

G

G

A

A

V

T

A

V

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

R

E

P

A

E

F

N

R

A

G

G

K

K

I

L

I

I

L

V

A

V

M

V

L

L

P
|
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K43), S266 (= S263), P293 (= P290)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (≠ T121), Q144 (≠ H143), K215 (≠ A212), G222 (= G219), A225 (≠ P222)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
47% identity, 98% coverage: 5:302/303 of query aligns to 6:306/309 of 7n2tA

query
sites
7n2tA

N

D

I

V

L

T

Q

E

T

L

I

I

G

G

N

N

T

T

P

P

V

L

I

V

R

Y

L

L

N

N

R

R

F

V

P

V

D

D

T

G

L

C

A

V

A

A

E

R

L

V

W

A

V

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

N

S

M

M

I

I

E

S

C

D

A

A

E

E

Q

N

R

K

G

G

E

L

L

I

K

T

P

P

G

G

D

E

T

S

I

V

-

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

F

I

I

A

A

Q

A

K

K

G

G

Y

Y

P

R

S

L

V

I

F

I

V

C

M

M

A

P

E

A

D

S

M

M

S

S

E

L

E

E

R

R

K

R

T

T

I

I

L

L

R

R

A

A

F

F

G

G

G

A

K

E

L

L

V

V

L

L

T

T

P

D

A

P

E

A

K

R

G

G

T

M

K

K

G

G

A

A

I

V

E

Q

E

K

A

A

K

E

L

I

A

K

A

A

K

K

N

T

G

P

W

N

F

S

F

Y

V

I

G

L

Q

Q

H

Q

F

F

-

E

N

N

P

P

D

A

N

N

P

P

N

K

A

I

H

H

-

Y

K

E

G

T

T

T

A

G

D

P

E

E

I

I

W

W

A

R

D

G

F

S

G

G

T

G

R

K

L

I

D

D

A

A

I

L

V

V

C

S

T

G

T

I

G

G

T

T

G

G

T

T

I

V

S

T

G

G

I

A

G

G

K

K

Y

Y

M

L

R

K

R

E

H

Q

N

N

P

P

E

N

I

I

Q

K

I

L

I

Y

A

G

T

V

E

E

P

P

A

V

D

E

S

S

P

A

I

I

L

L

S

S

Q

G

G

G

I

K

A

P

C

G

K

P

H

H

K

K

I

I

M

Q

G

G

T

I

A

G

P

A

G

G

F

F

I

I

P

P

D

G

I

V

L

L

D

D

T

V

K

N

I

L

Y

L

N

D

R

E

I

V

F

I

Q

Q

I

V

T

S

D

E

E

E

A

S

Y

I

D

E

T

T

T

A

R

K

R

L

L

L

A

A

Q

L

H

K

E

E

G

G

I

L

F

L

A

V

G

G

I

I

S

S

G

G

A

A

V

A

A

A

G

A

M

A

I

I

K
x
R

A

I

A

A

R

K

E
x
R

E

P

A

E

F

N

R

A

G

G

K

K

I

L

I

I

L

V

A

A

M

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

V

L

L

W

F

binding phosphate ion: R277 (≠ K273), R281 (≠ E277)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
46% identity, 99% coverage: 4:303/303 of query aligns to 7:313/323 of P0ABK5

query
sites
P0ABK5

D

D

N

N

I

S

L

L

Q

-

T

T

I

I

G

G

N

H

T

T

P

P

V

L

I

V

R

R

L

L

N

N

R

R

F

I

P

G

D

N

T

-

L

-

A

-

A

G

E

R

L

I

W

L

V

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K
|
K

D

C

R

R

P

I

A

G

L

A

N

N

M

M

I

I

E

W

C

D

A

A

E

E

Q

K

R

R

G

G

E

V

L

L

K

K

P

P

G

G

D

V

T

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

M

Y

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

K

S

L

V

T

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

I

E

E

R

R

K

R

T

K

I

L

L

L

R

K

A

A

F

L

G

G

G

A

K

N

L

L

V

V

L

L

T

T

P

E

A

G

E

A

K

K

G

G

T

M

K

K

G

G

A

A

I

I

E

Q

E

K

A

A

K

E

L

I

A

V

A

A

K

S

N

N

-

P

-

E

G

K

W

Y

F

L

V

L

G

Q

Q

Q

H

F

F

S

N

N

P

P

D

A

N

N

P

P

N

E

A

I

H

H

-

E

K

K

G

T

T

T

A

G

D

P

E

E

I

I

W

W

A

E

D

D

F

T

G

D

T

G

R

Q

L

V

D

D

A

V

I

F

V

I

C

A

T

G

T

V

G

G

T

T

G

G

T

T

I

L

S

T

G

G

I

V

G

S

K

R

Y

Y

M

I

R

K

-

G

-

T

R

K

H

G

N

K

P

T

E

D

I

L

Q

I

I

S

I

V

A

A

T

V

E

E

P

P

A

T

D

D

S

S

P

P

I

V

L

I

S

A

Q

Q

G

A

I

L

A

A

C

G

K

E

-

E

-

I

-

K

-

P

-

G

-

P

H

H

K

K

I

I

M

Q

G

G

T

I

A

G

P

A

G

G

F

F

I

I

P

P

D

A

I

N

L

L

D

D

T

L

K

K

I

L

Y

V

N

D

R

K

I

V

F

I

Q

G

I

I

T

T

T

N

D

E

E

E

A

A

Y

I

D

S

T

T

T

A

R

R

L

L

A

M

Q

E

H

E

E

E

G

G

I

I

F

L

A

A

G

G

I

I

S

S

G

G

A

A

V

V

A

A

G

A

M

A

I

L

K

K

A

L

A

Q

R

E

E

D

E

E

A

S

F

F

R

T

G

N

K

K

I

N

I

I

L

V

A

V

M

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

A

L

L

W

F

S

A

K42 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
45% identity, 99% coverage: 3:303/303 of query aligns to 5:313/323 of P0A1E3

query
sites
P0A1E3

Y

Y

D

E

N

D

I

N

L

S

Q

L

T

T

I

I

G

G

N

H

T

T

P

P

V

L

I

V

R

R

L

L

N

N

R

R

F

I

P

G

D

N

T

-

L

-

A

-

A

G

E

R

L

I

W

L

V

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K

K

D

C

R

R

P

I

A

G

L

A

N

N

M

M

I

I

E

W

C

D

A

A

E

E

Q

K

R

R

G

G

E

V

L

L

K

K

P

P

G

G

D

V

T

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

M

Y

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

K

S

L

V

T

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

I

E

E

R

R

K

R

T

K

I

L

L

L

R

K

A

A

F

L

G

G

G

A

K

N

L

L

V

V

L

L

T

T

P

E

A

G

E

A

K

K

G

G

T

M

K

K

G

G

A

A

I

I

E

Q

E

K

A

A

K

E

L

I

A

V

A

A

K

S

N

D

-

P

-

Q

G

K

W

Y

F

L

V

L

G

Q

Q

Q

H

F

F

S

N

N

P

P

D

A

N

N

P

P

N

E

A

I

H

H

-

E

K

K

G

T

T

T

A

G

D

P

E

E

I

I

W

W

A

E

D

D

F

T

G

D

T

G

R

Q

L

V

D

D

A

V

I

F

V

I

C

S

T

G

T

V

G

G

T

T

G

G

T

T

I

L

S

T

G

G

I

V

G

T

K

R

Y

Y

M

I

R

K

-

G

-

T

R

K

H

G

N

K

P

T

E

D

I

L

Q

I

I

T

I

V

A

A

T

V

E

E

P

P

A

T

D

D

S

S

P

P

I

V

L

I

S

A

Q

Q

G

A

I

L

A

A

C

G

K

E

-

E

-

I

-

K

-

P

-

G

-

P

H

H

K

K

I

I

M

Q

G

G

T

I

A

G

P

A

G

G

F

F

I

I

P

P

D

G

I

N

L

L

D

D

T

L

K

K

I

L

Y

I

N

D

R

K

I

V

F

V

Q

G

I

I

T

T

T

N

D

E

E

E

A

A

Y

I

D

S

T

T

T

A

R

R

L

L

A

M

Q

E

H

E

E

E

G

G

I

I

F

L

A

A

G

G

I

I

S
|
S

S

S

G

G

A

A

V

V

A

A

G

A

M

A

I

L

K

K

A

L

A

Q

R

E

E

D

E

E

A

S

F

F

R

T

G

N

K

K

I

N

I

I

L

V

A

V

M

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

A

L

L

W

F

S

A

N72 (= N73) binding pyridoxal 5'-phosphate
S273 (= S263) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
45% identity, 99% coverage: 3:303/303 of query aligns to 6:314/321 of 6z4nAAA

query
sites
6z4nAAA

Y

Y

D

E

N

D

I

N

L

S

Q

L

T

T

I

I

G

G

N

H

T

T

P

P

V

L

I

V

R

R

L

L

N

N

R

R

F

I

P

G

D

N

T

-

L

-

A

-

A

G

E

R

L

I

W

L

V

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K
|
K

D

C

R

R

P

I

A

G

L

A

N

N

M

M

I

I

E

W

C

D

A

A

E

E

Q

K

R

R

G

G

E

V

L

L

K

K

P

P

G

G

D

V

T

E

I

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

M

Y

I

V

A

A

Q

A

K

R

G

G

Y

Y

P

K

S

L

V

T

F

L

V

T

M

M

A

P

E

E

D

T

M

M

S

S

E

I

E

E

R

R

K

R

T

K

I

L

L

L

R

K

A

A

F

L

G

G

G

A

K

N

L

L

V

V

L

L

T

T

P

E

A

G

E

A

K

K

G

G

T

M

K

K

G

G

A

A

I

I

E

Q

E

K

A

A

K

E

L

I

A

V

A

A

K

S

N

D

-

P

-

Q

G

K

W

Y

F

L

V

L

G

Q

Q
|
Q

H
x
F

F

S

N

N

P

P

D

A

N

N

P

P

N

E

A

I

H

H

-

E

K

K

G

T

T

T

A

G

D

P

E

E

I

I

W

W

A

E

D

D

F

T

G

D

T

G

R

Q

L

V

D

D

A

V

I

F

V

I

C

S

T

G

T
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

S

T

G

G

I

V

G

T

K

R

Y

Y

M

I

R

K

-

G

-

T

R

K

H

G

N

K

P

T

E

D

I

L

Q

I

I

T

I

V

A

A

T

V

E

E

P

P

A

T

D

D

S

S

P

P

I

V

L

I

S

A

Q

Q

G

A

I

L

A

A

C

G

K

E

-

E

-

I

-

K

-

P

-

G

-

P

H

H

K

K

I

I

M
x
Q

G
|
G

T
x
I

A

G

P
x
A

G

G

F

F

I

I

P

P

D

G

I

N

L

L

D

D

T

L

K

K

I

L

Y

I

N

D

R

K

I

V

F

V

Q

G

I

I

T

T

T

N

D

E

E

E

A

A

Y

I

D

S

T

T

T

A

R

R

L

L

A

M

Q

E

H

E

E

E

G

G

I

I

F

L

A

A

G

G

I

I

S
|
S

S

S

G

G

A

A

V

V

A

A

G

A

M

A

I

L

K

K

A

L

A

Q

R

E

E

D

E

E

A

S

F

F

R

T

G

N

K

K

I

N

I

I

L

V

A

V

M

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

D

A

L

L

W

F

S

A

binding pyridoxal-5'-phosphate: K43 (= K43), N73 (= N73), V177 (≠ T174), G178 (= G175), T179 (= T176), G180 (= G177), T182 (= T179), G230 (= G219), S274 (= S263), P301 (= P290)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K43), T70 (= T70), G72 (= G72), N73 (= N73), T74 (= T74), Q144 (= Q142), F145 (≠ H143), Q229 (≠ M218), G230 (= G219), I231 (≠ T220), A233 (≠ P222)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
47% identity, 97% coverage: 9:303/303 of query aligns to 6:302/302 of 2efyA

query
sites
2efyA

T

A

I

I

G

G

N

K

T

T

P

P

V

V

I

V

R

R

L

L

N

A

R

K

F

V

P

V

D

E

T

P

L

D

A

M

A

A

E

E

L

V

W

W

V

V

K

K

L

L

E

E

S

G

F

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

E

K

C

D

A

A

E

E

Q

E

R

R

G

G

E

I

L

L

K

R

P

P

-

G

-

S

G

G

D

Q

T

V

I

I

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

I

I

A

A

Q

S

K

R

G

G

Y

Y

P

R

S

L

V

I

F

L

V

T

M

M

A

P

E

A

D

Q

M

M

S

S

E

E

R

R

K

K

T

R

I

V

L

L

R

K

A

A

F

F

G

G

G

A

K

E

L

L

V

V

L

L

T

T

P

D

A

P

E

E

K

R

G

R

T

M

K

L

G

A

A

A

I

R

E

E

A

A

K

L

K

R

L

L

A

K

A

E

K

E

N

L

G

G

W

A

F

F

F

M

V

P

G

D

Q
|
Q

H

F

F

K

N

N

P

P

D

A

N

N

P

V

N

R

A

A

H

H

-

Y

K

E

G

T

T

T

A

G

D

P

E

E

I

L

W

Y

A

E

D

A

F

L

G

E

T

G

R

R

L

I

D

D

A

A

I

F

V

V

C
x
Y

T

G

T

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

I

V

G

G

K

R

Y

Y

M

L

R

K

R

E

H

R

N

I

P

P

E

H

I

V

Q

K

I

V

I

I

A

A

T

V

E
|
E

P

P

A

A

D

R

S
|
S

P

N

I

V

L

L

S

S

Q

G

G

G

I

K

A

M

C

G

K

Q

H

H

K

G

I

F

M

Q

G
|
G

T
x
M

A

G

P
|
P

G

G

F

F

I

I

P

P

D

E

I

N

L

L

D

D

T

L

K

S

I

L

Y

L

N

D

R

G

I

V

F

I

Q

Q

I

V

T

W

T

E

D

E

E

D

A

A

Y

F

D

P

T

L

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

F

A

L

G

G

I

M

S
|
S

S

S

G

G

A

G

A

I

V

V

A

W

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

E

E

L

A

G

F

-

R

P

G

G

K

K

I

R

I

V

L

A

A

C

M

I

L

S

P
|
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

T

T

D

P

L

L

W

Y

S

A

active site: K40 (= K43), S70 (= S71), E200 (= E200), S204 (= S204), S263 (= S263)
binding 5-oxohexanoic acid: T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G175), G219 (= G219), M220 (≠ T220), P222 (= P222)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ C172), G175 (= G175), T176 (= T176), G177 (= G177), T179 (= T179), G219 (= G219), S263 (= S263), P289 (= P290), D290 (= D291)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
47% identity, 97% coverage: 9:303/303 of query aligns to 6:302/302 of 2ecqA

query
sites
2ecqA

T

A

I

I

G

G

N

K

T

T

P

P

V

V

I

V

R

R

L

L

N

A

R

K

F

V

P

V

D

E

T

P

L

D

A

M

A

A

E

E

L

V

W

W

V

V

K

K

L

L

E

E

S

G

F

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

E

K

C

D

A

A

E

E

Q

E

R

R

G

G

E

I

L

L

K

R

P

P

-

G

-

S

G

G

D

Q

T

V

I

I

I

V

E

E

P

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

I

I

A

A

Q

S

K

R

G

G

Y

Y

P

R

S

L

V

I

F

L

V

T

M

M

A

P

E

A

D

Q

M

M

S

S

E

E

R

R

K

K

T

R

I

V

L

L

R

K

A

A

F

F

G

G

G

A

K

E

L

L

V

V

L

L

T

T

P

D

A

P

E

E

K

R

G

R

T

M

K

L

G

A

A

A

I

R

E

E

A

A

K

L

K

R

L

L

A

K

A

E

K

E

N

L

G

G

W

A

F

F

F

M

V

P

G

D

Q
|
Q

H

F

F

K

N

N

P

P

D

A

N

N

P

V

N

R

A

A

H

H

-

Y

K

E

G

T

T

T

A

G

D

P

E

E

I

L

W

Y

A

E

D

A

F

L

G

E

T

G

R

R

L

I

D

D

A

A

I

F

V

V

C
x
Y

T
x
G

T

S

G
|
G

T
|
T

G

G

T
|
T

I

I

S

T

G

G

I

V

G

G

K

R

Y

Y

M

L

R

K

R

E

H

R

N

I

P

P

E

H

I

V

Q

K

I

V

I

I

A

A

T

V

E
|
E

P

P

A

A

D

R

S
|
S

P

N

I

V

L

L

S

S

Q

G

G

G

I

K

A

M

C

G

K

Q

H

H

K

G

I

F

M

Q

G
|
G

T

M

A

G

P

P

G

G

F

F

I

I

P

P

D

E

I

N

L

L

D

D

T

L

K

S

I

L

Y

L

N

D

R

G

I

V

F

I

Q

Q

I

V

T

W

T

E

D

E

E

D

A

A

Y

F

D

P

T

L

T

A

R

R

L

L

A

A

Q

R

H

E

E

E

G

G

I

L

F

F

A

L

G

G

I

M

S
|
S

S

S

G

G

A

G

A

I

V

V

A

W

G

A

M

A

I

L

K

Q

A

V

A

A

R

R

E

E

E

L

A

G

F

-

R

P

G

G

K

K

I

R

I

V

L

A

A

C

M

I

L

S

P
|
P

D

D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

T

T

D

P

L

L

W

Y

S

A

active site: K40 (= K43), S70 (= S71), E200 (= E200), S204 (= S204), S263 (= S263)
binding (3s)-3-hydroxybutanoic acid: K40 (= K43), G71 (= G72), T73 (= T74), Q141 (= Q142), G219 (= G219)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ C172), G173 (≠ T173), G175 (= G175), T176 (= T176), T179 (= T179), G219 (= G219), S263 (= S263), P289 (= P290)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
47% identity, 97% coverage: 9:303/303 of query aligns to 6:302/302 of 2ecoA

query
sites
2ecoA

T

A

I

I

G

G

N

K

T

T

P

P

V

V

I

V

R

R

L

L

N

A

R

K

F

V

P

V

D

E

T

P

L

D

A

M

A

A

E

E

L

V

W

W

V

V

K

K

L

L

E

E

S

G

F

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

N

Y

M

M

I

I

E

K

C

D

A

A

E

E

Q

E

R

R

G

G

E

I

L

L

K

R

P

P

-

G

-

S

G

G

D

Q

T

V

I

I

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

I

I

A

A

Q

S

K

R

G

G

Y

Y

P

R

S

L

V

I

F

L

V

T

M

M

A

P

E

A

D

Q

M

M

S

S

E

E

R

R

K

K

T

R

I

V

L

L

R

K

A

A

F

F

G

G

G

A

K

E

L

L

V

V

L

L

T

T

P

D

A

P

E

E

K

R

G

R

T

M

K

L

G

A

A

A

I

R

E

E

A

A

K

L

K

R

L

L

A

K

A

E

K

E

N

L

G

G

W

A

F

F

F

M

V

P

G

D

Q
|
Q

H

F

F

K

N

N

P

P

D

A

N

N

P

V

N

R

A

A

H

H

-

Y

K

E