SitesBLAST
Comparing WP_037568880.1 NCBI__GCF_000744815.1:WP_037568880.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
O87590 HTH-type transcriptional regulator CelR from Thermobifida fusca (Thermomonospora fusca) (see paper)
31% identity, 98% coverage: 3:329/334 of query aligns to 8:339/340 of O87590
- A55 (≠ S50) mutation to T: Decreases binding to DNA and forms a less stable complex with the celE promoter in the presence of cellobiose.
5yszA Transcriptional regulator celr-cellobiose complex (see paper)
30% identity, 98% coverage: 3:329/334 of query aligns to 3:326/327 of 5yszA
- binding beta-D-glucopyranose: E68 (vs. gap), F70 (≠ V76), Y71 (≠ I77), S119 (≠ D123), S119 (≠ D123), G140 (≠ I144), D155 (= D157), I156 (≠ L158), N158 (≠ F160), R192 (≠ T199), R192 (≠ T199), F217 (≠ G221), D271 (≠ C275), Q288 (≠ I292), M293 (= M297)
1rzrG Crystal structure of transcriptional regulator-phosphoprotein-DNA complex (see paper)
25% identity, 99% coverage: 2:330/334 of query aligns to 3:331/332 of 1rzrG
- binding : T4 (= T3), I5 (≠ L4), S15 (= S14), M16 (≠ A15), A17 (≠ S16), T18 (= T17), S20 (= S19), R21 (≠ Y20), N24 (≠ S23), N28 (≠ S27), V29 (≠ I28), K30 (≠ S29), Y46 (= Y45), P48 (= P47), N49 (= N48), A52 (= A51), R53 (= R52), L55 (= L54), A56 (= A55), A56 (= A55), S57 (= S56)
1rzrA Crystal structure of transcriptional regulator-phosphoprotein-DNA complex (see paper)
25% identity, 99% coverage: 2:330/334 of query aligns to 3:331/332 of 1rzrA
- binding magnesium ion: N101 (≠ G103), D103 (= D105)
- binding : I5 (≠ L4), V14 (= V13), S15 (= S14), M16 (≠ A15), T18 (= T17), S20 (= S19), R21 (≠ Y20), R21 (≠ Y20), N24 (≠ S23), N28 (≠ S27), V29 (≠ I28), K30 (≠ S29), T33 (= T32), Y46 (= Y45), P48 (= P47), N49 (= N48), A52 (= A51), R53 (= R52), L55 (= L54), A56 (= A55), A56 (= A55)
3oqoA Ccpa-hpr-ser46p-syn cre (see paper)
25% identity, 93% coverage: 2:313/334 of query aligns to 3:313/333 of 3oqoA
- binding : T4 (= T3), I5 (≠ L4), V14 (= V13), S15 (= S14), A17 (≠ S16), T18 (= T17), S20 (= S19), R21 (≠ Y20), N24 (≠ S23), N28 (≠ S27), V29 (≠ I28), K30 (≠ S29), T33 (= T32), Y46 (= Y45), N49 (= N48), A52 (= A51), R53 (= R52), L55 (= L54), A56 (= A55), K58 (≠ S57)
Sites not aligning to the query:
2jcgA Apo form of the catabolite control protein a (ccpa) from bacillus megaterium, with the DNA binding domain (see paper)
24% identity, 93% coverage: 2:310/334 of query aligns to 1:300/322 of 2jcgA
P0ACP7 HTH-type transcriptional repressor PurR; Pur regulon repressor; Purine nucleotide synthesis repressor from Escherichia coli (strain K12) (see 5 papers)
25% identity, 99% coverage: 1:329/334 of query aligns to 1:330/341 of P0ACP7
- M1 (= M1) modified: Initiator methionine, Removed
- K55 (≠ A55) mutation to A: Decrease in operator DNA affinity.
- Y73 (= Y73) binding hypoxanthine
- W147 (≠ L146) mutation to A: 14-fold increase in corepressor affinity. Large increase in repressor activity.; mutation to F: Large decrease in corepressor affinity and in repressor activity.; mutation to R: 8-fold increase in corepressor affinity. Large increase in repressor activity.
- R190 (= R191) binding hypoxanthine; mutation to A: Functional repressor. Corepressor specificity is expanded since it allows binding of adenine and 6-methylpurine.; mutation to Q: Functional repressor. Corepressor specificity is expanded since it allows binding of adenine.
- T192 (= T193) binding hypoxanthine
- F221 (= F223) binding hypoxanthine
- D275 (≠ C275) binding hypoxanthine
1wetA Structure of the purr-guanine-purf operator complex (see paper)
25% identity, 98% coverage: 3:329/334 of query aligns to 1:328/338 of 1wetA
- binding guanine: Y71 (= Y73), F72 (≠ I77), R188 (= R191), T190 (= T193), R194 (≠ T199), F219 (= F223), D273 (≠ C275)
- binding : S12 (= S14), T14 (≠ S16), T15 (= T17), R24 (= R26), V26 (≠ I28), A27 (≠ S29), T30 (= T32), L52 (= L54), K53 (≠ A55)
1jftA Purine repressor mutant-hypoxanthine-purf operator complex (see paper)
25% identity, 98% coverage: 3:329/334 of query aligns to 2:329/340 of 1jftA
- binding hypoxanthine: Y72 (= Y73), F73 (≠ I77), R189 (= R191), T191 (= T193), R195 (≠ T199), F220 (= F223), D274 (≠ C275)
- binding : S13 (= S14), T15 (≠ S16), T16 (= T17), R25 (= R26), V27 (≠ I28), A28 (≠ S29), T31 (= T32), L53 (= L54), K54 (≠ A55)
2pubA Crystal structure of the laci family member, purr, bound to dna: minor groove binding by alpha helices (see paper)
25% identity, 98% coverage: 3:329/334 of query aligns to 1:328/338 of 2pubA
- binding adenine: Y71 (= Y73), F72 (≠ I77), T190 (≠ S187), R194 (= R191), F219 (= F223), D273 (≠ C275)
- binding : S12 (= S14), T14 (≠ S16), T15 (= T17), R24 (= R26), V26 (≠ I28), A27 (≠ S29), T30 (= T32), L52 (= L54), K53 (≠ A55)
2puaA Crystal structure of the laci family member, purr, bound to dna: minor groove binding by alpha helices (see paper)
25% identity, 98% coverage: 3:329/334 of query aligns to 2:329/339 of 2puaA
- binding 6-methylpurine: Y72 (= Y73), F73 (≠ I77), T191 (≠ S187), R195 (= R191), F220 (= F223), D274 (≠ C275)
- binding : S13 (= S14), T15 (≠ S16), T16 (= T17), R25 (= R26), V27 (≠ I28), A28 (≠ S29), T31 (= T32), L53 (= L54), K54 (≠ A55)
P21867 HTH-type transcriptional regulator RafR; Raffinose operon repressor; raf repressor from Escherichia coli (see paper)
30% identity, 58% coverage: 2:195/334 of query aligns to 1:200/336 of P21867
- M1 (≠ V2) modified: Initiator methionine, Removed
- A50 (= A51) mutation to E: Loss of activity.
7ce1K Complex structure of transcription factor sghr with its cognate DNA (see paper)
23% identity, 93% coverage: 3:313/334 of query aligns to 4:314/333 of 7ce1K
- binding : L5 (= L4), G15 (≠ S14), T17 (≠ S16), T18 (= T17), K24 (≠ S23), D28 (≠ S27), I29 (= I28), G30 (≠ S29), T33 (= T32), Y46 (= Y45), P48 (= P47), N49 (= N48), G52 (≠ A51), V53 (≠ R52), L55 (= L54), R56 (≠ A55), R56 (≠ A55), R173 (≠ H170), E279 (≠ S278), N282 (≠ L281), W283 (≠ Q282)
Sites not aligning to the query:
1lbgA Lactose operon repressor bound to 21-base pair symmetric operator DNA, alpha carbons only (see paper)
24% identity, 99% coverage: 2:332/334 of query aligns to 4:331/357 of 1lbgA
2o20B Crystal structure of transcription regulator ccpa of lactococcus lactis (see paper)
25% identity, 75% coverage: 54:305/334 of query aligns to 1:248/275 of 2o20B
1efaA Crystal structure of the lac repressor dimer bound to operator and the anti-inducer onpf (see paper)
24% identity, 99% coverage: 2:330/334 of query aligns to 3:328/328 of 1efaA
- binding 2-nitrophenyl beta-D-fucopyranoside: A74 (≠ V76), D148 (≠ G145), F160 (≠ L158), S192 (≠ Q190), R196 (≠ G194), N245 (= N247), D273 (≠ C275), F292 (≠ A294)
- binding : L5 (= L4), Y6 (≠ A5), S15 (= S14), Y16 (≠ A15), Q17 (≠ S16), T18 (= T17), S20 (= S19), R21 (≠ Y20), N24 (≠ S23), Y46 (= Y45), N49 (= N48), A52 (= A51), Q53 (≠ R52), L55 (= L54), A56 (= A55), A56 (= A55), K58 (≠ S57)
3k4hA Crystal structure of putative transcriptional regulator laci from bacillus cereus subsp. Cytotoxis nvh 391-98
24% identity, 75% coverage: 75:323/334 of query aligns to 20:270/282 of 3k4hA
- binding alpha-D-glucopyranose: F21 (≠ V76), F21 (≠ V76), F22 (≠ I77), L70 (≠ M122), I92 (= I144), G93 (= G145), D106 (= D157), N107 (≠ L158), N109 (≠ F160), V139 (≠ Q190), R143 (≠ G194), F168 (vs. gap), D194 (≠ N247), N222 (≠ C275)
7ce1T Complex structure of transcription factor sghr with its cognate DNA (see paper)
26% identity, 63% coverage: 3:212/334 of query aligns to 4:204/292 of 7ce1T
- binding : T4 (= T3), L5 (= L4), G15 (≠ S14), T17 (≠ S16), T18 (= T17), K24 (≠ S23), D28 (≠ S27), I29 (= I28), G30 (≠ S29), T33 (= T32), P48 (= P47), N49 (= N48), V53 (≠ R52), L55 (= L54), R56 (≠ A55), R56 (≠ A55)
2pe5A Crystal structure of the lac repressor bound to onpg in repressed state (see paper)
24% identity, 99% coverage: 2:330/334 of query aligns to 3:328/328 of 2pe5A
- binding 2-nitrophenyl beta-D-galactopyranoside: L72 (≠ V74), A74 (≠ V76), P75 (≠ I77), F160 (≠ L158), R196 (≠ G194), W219 (≠ F223), N245 (= N247), D273 (≠ C275), Q290 (≠ I292), F292 (≠ A294)
- binding : T4 (= T3), L5 (= L4), S15 (= S14), Y16 (≠ A15), Q17 (≠ S16), Q17 (≠ S16), T18 (= T17), R21 (≠ Y20), S30 (= S29), T33 (= T32), Y46 (= Y45), P48 (= P47), N49 (= N48), A52 (= A51), Q53 (≠ R52), L55 (= L54), A56 (= A55)
7ce1S Complex structure of transcription factor sghr with its cognate DNA (see paper)
26% identity, 48% coverage: 3:162/334 of query aligns to 4:165/259 of 7ce1S
- binding : L5 (= L4), G15 (≠ S14), T17 (≠ S16), T17 (≠ S16), T18 (= T17), S20 (= S19), K24 (≠ S23), D28 (≠ S27), I29 (= I28), G30 (≠ S29), T33 (= T32), Y46 (= Y45), N49 (= N48), L55 (= L54), R56 (≠ A55), R56 (≠ A55)
Query Sequence
>WP_037568880.1 NCBI__GCF_000744815.1:WP_037568880.1
MVTLAEVAARAGVSASTVSYVISGKRSISQATQDRVLRAVTELGYHPNASARALASSRSN
VIALMVPLRSDMYVPVIMEIVMAIAATARAHGQDILLLTGGEGPDGVRRVAGTGLADAII
LMDVELDDKRIPVLREASMPAVLIGLPSDTADLTCIDLDFEATGAACADHLADLGHRDIA
LIGESRSVYQRRTGFAARTLEGFRQRARARGVRAVHRPCEGTFESTAGVLTRILEERPET
SGFVVQNEAAIRPLLTLLRQFGRVVPEDASVVAICPESVALQSSPQLTSVSIPAERMGRG
ALELVMAHLSGRPTAGTTLIAPELAVRESSLSAG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory